keyword
MENU ▼
Read by QxMD icon Read
search

Glutamine

keyword
https://www.readbyqxmd.com/read/29909456/construction-of-a-thermoresponsive-magnetic-porous-polymer-membrane-enzyme-reactor-for-glutaminase-kinetics-study
#1
Liping Zhao, Juan Qiao, Meyong Hee Moon, Li Qi
Fabrication of polymer membranes with nanopores and a confinement effect toward enzyme immobilization has been an enabling endeavor. In the work reported here, an enzyme reactor based on a thermoresponsive magnetic porous block copolymer membrane was designed and constructed. Reversible addition-fragmentation chain transfer polymerization was used to synthesize the block copolymer, poly(maleic anhydride-styrene-N-isopropylacrylamide), with poly(N-isopropylacrylamide) as the thermoresponsive moiety. The self-assembly property of the block copolymer was used for preparation of magnetic porous thin film matrices with iron oxide nanoparticles...
June 16, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29909287/prazosin-induced-lysosomal-tubulation-interferes-with-cytokinesis-and-the-endocytic-sorting-of-the-tumour-antigen-cd98hc
#2
Robert Fuchs, Anika Stracke, Viktoria Holzmann, Gerfried Luschin-Ebengreuth, Nathalie Meier-Allard, Nadine Ebner, Teresa Maria Lassacher, Markus Absenger-Novak, Eleonore Fröhlich, Matthias Schittmayer, Sara Cano Crespo, Manuel Palacin, Beate Rinner, Ruth Birner-Gruenberger
The quinazoline based drug prazosin (PRZ) is a potent inducer of apoptosis in human cancer cells. We recently reported that PRZ enters cells via endocytosis and induces tubulation of the endolysosomal system. In a proteomics approach aimed at identifying potential membrane proteins with binding affinity to quinazolines, we detected the oncoprotein CD98hc. We confirmed shuttling of CD98hc towards lysosomes and upregulation of CD98hc expression in PRZ treated cells. Gene knockout (KO) experiments revealed that endocytosis of PRZ still occurs in the absence of CD98hc - suggesting that PRZ does not enter the cell via CD98hc but misroutes the protein towards tubular lysosomes...
June 14, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29908254/the-neurotoxin-diethyl-dithiophosphate-impairs-glutamate-transport-in-cultured-bergmann-glia-cells
#3
Tatiana N Olivares-Bañuelos, Isabel Martínez-Hernández, Luisa C Hernández-Kelly, Donají Chi-Castañeda, Libia Vega, Arturo Ortega
Glutamate, the main excitatory neurotransmitter in the vertebrate Central Nervous System, is involved in almost every aspect of brain physiology, and its signaling properties are severely affected in most neurodegenerative diseases. This neurotransmitter has to be efficiently removed from the synaptic cleft in order to prevent an over-stimulation of glutamate receptors that leads to neuronal death. Specific sodium-dependent membrane transporters, highly enriched in glial cells, elicit the clearance of glutamate...
June 13, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29908151/vaccination-with-a-cd4-and-cd8-t-cell-epitopes-based-recombinant-chimeric-protein-derived-from-leishmania-infantum-proteins-confers-protective-immunity-against-visceral-leishmaniasis
#4
Daniel S Dias, Patrícia A F Ribeiro, Vívian T Martins, Daniela P Lage, Lourena E Costa, Miguel A Chávez-Fumagalli, Fernanda F Ramos, Thaís T O Santos, Fernanda Ludolf, Jamil S Oliveira, Tiago A O Mendes, Eduardo S Silva, Alexsandro S Galdino, Mariana C Duarte, Bruno M Roatt, Daniel Menezes-Souza, Antonio L Teixeira, Eduardo A F Coelho
Vaccination seems to be the best approach to control visceral leishmaniasis (VL). Resistance against infection is based on the development of a Th1 immune response characterized by the production of interferons-γ (IFN-γ), interleukin-12 (IL-12), granulocyte-macrophage-colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α), among others. A number of antigens have been tested as potential targets against the disease; few of them are able to stimulate human immune cells. In the present study, 1 prediction of MHC class I and II molecules-specific epitopes in the amino acid sequences of 3 Leishmania proteins: 1 hypothetical, prohibitin, and small glutamine-rich tetratricopeptide repeat-containing proteins, was performed using bioinformatics tools, and a T-cell epitopes-based recombinant chimeric protein was constructed, synthetized and purified to be evaluated in invitro and in vivo experiments...
May 22, 2018: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29907927/-1-h-mrs-spectroscopy-in-preclinical-autosomal-dominant-alzheimer-disease
#5
Elizabeth Joe, Luis D Medina, John M Ringman, Joseph O'Neill
1 H magnetic resonance spectroscopy (MRS) can reveal changes in brain biochemistry in vivo in humans and has been applied to late onset Alzheimer disease (AD). Carriers of mutations for autosomal dominant Alzheimer disease (ADAD) may show changes in levels of metabolites prior to the onset of clinical symptoms. Proton MR spectra were acquired at 1.5 T for 16 cognitively asymptomatic or mildly symptomatic mutation carriers (CDR < 1) and 11 non-carriers as part of a comprehensive cross-sectional study of preclinical ADAD...
June 16, 2018: Brain Imaging and Behavior
https://www.readbyqxmd.com/read/29907529/kinetic-analysis-on-precursors-for-iturin-a-production-from-bacillus-amyloliquefaciens-bpd1
#6
Jiun-Yan Wu, Jen-Hung Liao, Chwen-Jen Shieh, Feng-Chia Hsieh, Yung-Chuan Liu
In this study, the precursor effect for iturin A production was quantitatively analyzed. A strain identified as Bacillus amyloliquefaciens BPD1 (Ba-BPD1) was selected due to its ability to produce iturin A. The enhancement of iturin A production in a submerged culture was tested using various additives, including palmitic acid, oils, and complex amino acids. Among these, complex amino acids triggered the highest yield at 559 mg/L. The respective amino acids that contribute to the structure of iturin A were used as precursors...
June 12, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29905857/gfpt1-deficiency-in-muscle-leads-to-myasthenia-and-myopathy-in-mice
#7
Yasmin Issop, Denisa Hathazi, Muzamil Majid Khan, Rüdiger Rudolf, Joachim Weis, Sally Spendiff, Clarke R Slater, Andreas Roos, Hanns Lochmüller
Glutamine-fructose-6-phosphate transaminase 1 (GFPT1) is the rate-limiting enzyme in the hexosamine biosynthetic pathway which yields precursors required for protein and lipid glycosylation. Mutations in GFPT1 and other genes downstream of this pathway cause congenital myasthenic syndrome (CMS) characterised by fatigable muscle weakness due to impaired neurotransmission. The precise pathomechanisms at the neuromuscular junction (NMJ) due to a deficiency in GFPT1 is yet to be discovered. One of the challenges we face is the viability of Gfpt1 -/- knockout mice...
June 14, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29904952/baicalin-effects-on-rats-with-spinal-cord-injury-by-anti-inflammatory-and-regulating-the-serum-metabolic-disorder
#8
Shufeng Kang, Shizhao Liu, Hongzhu Li, Dapeng Wang, Xiangbei Qi
Baicalin had neuroprotective effects on inhibiting neuronal cell apoptosis induced by spinal cord ischemic injury. This study aimed to explore the protective effects of Baicalin on rats with spinal cord injury (SCI) and its mechanism of action. The recovery of spinal cord nerve function in rats was evaluated by the Basso, Beattie, and Bresnahan (BBB) score and the combine behavioral score (CBS). The expressions of cytokines tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 were detected by the enzyme-linked immunosorbent assay method...
June 15, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29904050/amino-acid-dependent-cmyc-expression-is-essential-for-nk-cell-metabolic-and-functional-responses-in-mice
#9
Róisín M Loftus, Nadine Assmann, Nidhi Kedia-Mehta, Katie L O'Brien, Arianne Garcia, Conor Gillespie, Jens L Hukelmann, Peter J Oefner, Angus I Lamond, Clair M Gardiner, Katja Dettmer, Doreen A Cantrell, Linda V Sinclair, David K Finlay
Natural killer (NK) cells are lymphocytes with important anti-tumour functions. Cytokine activation of NK cell glycolysis and oxidative phosphorylation (OXPHOS) are essential for robust NK cell responses. However, the mechanisms leading to this metabolic phenotype are unclear. Here we show that the transcription factor cMyc is essential for IL-2/IL-12-induced metabolic and functional responses in mice. cMyc protein levels are acutely regulated by amino acids; cMyc protein is lost rapidly when glutamine is withdrawn or when system L-amino acid transport is blocked...
June 14, 2018: Nature Communications
https://www.readbyqxmd.com/read/29899358/glutamine-89-is-a-key-residue-in-the-allosteric-modulation-of-human-serine-racemase-activity-by-atp
#10
Andrea V Canosa, Serena Faggiano, Marialaura Marchetti, Stefano Armao, Stefano Bettati, Stefano Bruno, Riccardo Percudani, Barbara Campanini, Andrea Mozzarelli
Serine racemase (SR) catalyses two reactions: the reversible racemisation of L-serine and the irreversible dehydration of L- and D-serine to pyruvate and ammonia. SRs are evolutionarily related to serine dehydratases (SDH) and degradative threonine deaminases (TdcB). Most SRs and TdcBs - but not SDHs - are regulated by nucleotides. SR binds ATP cooperatively and the nucleotide allosterically stimulates the serine dehydratase activity of the enzyme. A H-bond network comprising five residues (T52, N86, Q89, E283 and N316) and water molecules connects the active site with the ATP-binding site...
June 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29898596/metabolomics-reveals-how-cucumber-cucumis-sativus-reprograms-metabolites-to-cope-with-silver-ions-and-silver-nanoparticle-induced-oxidative-stress
#11
Huiling Zhang, Wenchao Du, Jose R Peralta-Videa, Jorge L Gardea-Torresdey, Jason C White, Arturo A Keller, Hongyan Guo, Rong Ji, Lijuan Zhao
Due to their well-known antifungal activity, the intentional use of Ag nanoparticle (NPs) as sustainable nano-fungicides is expected to increase in agriculture. However, the impacts of AgNPs on plants must be critically evaluated to guarantee their safe use in food production. In this study, 4-week-old cucumber (Cucumis sativus) plants received a foliar application of AgNPs (4 or 40 mg per plant) or Ag+ (0.04 or 0.4 mg per plant) for seven days. Gas chromatography-mass spectrometry (GC-MS) based non-target metabolomics enabled the identification and quantification of 268 metabolites in cucumber leaves...
June 14, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29896109/dan-lou-prescription-inhibits-foam-cell-formation-induced-by-ox-ldl-via-the-tlr4-nf-%C3%AE%C2%BAb-and-ppar%C3%AE-signaling-pathways
#12
Li-Na Gao, Xin Zhou, Yu-Ren Lu, Kefeng Li, Shan Gao, Chun-Quan Yu, Yuan-Lu Cui
Atherosclerosis is the major worldwide cause of mortality for patients with coronary heart disease. Many traditional Chinese medicine compound prescriptions for atherosclerosis treatment have been tried in patients. Dan-Lou prescription, which is improved from Gualou-Xiebai-Banxia decoction, has been used to treat chest discomfort (coronary atherosclerosis) for approximately 2,000 years in China. Although the anti-inflammatory activities of Dan-Lou prescription have been proposed previously, the mechanism remains to be explored...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29895729/sources-and-fates-of-carbamyl-phosphate-a-labile-energy-rich-molecule-with-multiple-facets
#13
REVIEW
Dashuang Shi, Ljubica Caldovic, Mendel Tuchman
Carbamyl phosphate (CP) is well-known as an essential intermediate of pyrimidine and arginine/urea biosynthesis. Chemically, CP can be easily synthesized from dihydrogen phosphate and cyanate. Enzymatically, CP can be synthesized using three different classes of enzymes: (1) ATP-grasp fold protein based carbamyl phosphate synthetase (CPS); (2) Amino-acid kinase fold carbamate kinase (CK)-like CPS (anabolic CK or aCK); and (3) Catabolic transcarbamylase. The first class of CPS can be further divided into three different types of CPS as CPS I, CPS II, and CPS III depending on the usage of ammonium or glutamine as its nitrogen source, and whether N -acetyl-glutamate is its essential co-factor...
June 12, 2018: Biology
https://www.readbyqxmd.com/read/29895661/l-glutamine-for-sickle-cell-anemia-more-questions-than-answers
#14
Charles T Quinn
In 2017, the Food and Drug Administration (FDA) approved two medications for sickle cell anemia (SCA): hydroxyurea for children (≥2 years of age) and L-glutamine for children and adults (≥5 years). The approval of hydroxyurea for children was long overdue, having been authorized by the FDA for adults in 1998 and by the European Medicines Agency for adults and children in 2007, but the approval of L-glutamine was a surprise to many in the field. There are few published studies of L-glutamine as a treatment for SCA, so all can be reviewed in this brief manuscript...
June 12, 2018: Blood
https://www.readbyqxmd.com/read/29893645/changes-in-brain-metabolite-concentrations-after-neonatal-hypoxic-ischemic-encephalopathy
#15
Jun Shibasaki, Noriko Aida, Naho Morisaki, Moyoko Tomiyasu, Yuri Nishi, Katsuaki Toyoshima
Purpose To investigate the time-course changes and predictive utility of brain metabolite concentrations in neonatal hypoxic-ischemic encephalopathy (HIE). Materials and Methods Sixty-eight neonates (age, 35-41 gestational weeks) with HIE were admitted to a neonatal intensive care unit between September 2009 and March 2016 and examined by using proton MR spectroscopy at 18-96 hours (n = 25) and 7-14 days (n = 64) after birth (35-43 postmenstrual weeks) to estimate metabolite concentrations in the deep gray matter...
June 12, 2018: Radiology
https://www.readbyqxmd.com/read/29892306/multiple-resistance-evolution-in-bipyridylium-resistant-epilobium-ciliatum-after-recurrent-selection
#16
Berhoz K Tahmasebi, Ricardo Alcántara-de la Cruz, Esteban Alcántara, Joel Torra, José A Domínguez-Valenzuela, Hugo E Cruz-Hipólito, Antonia M Rojano-Delgado, Rafael De Prado
The use of herbicides with different modes of action is the primary strategy used to control weeds possessing resistance to a single mechanism of action (MOA). However, this practice can lead to selection for generalist resistance mechanisms and may cause resistance to all MOAs. In this research, we characterized the resistance to diquat/paraquat (bipyridiliums) in an Epilobium ciliatum biotype (R1) collected in an olive orchard from Chile, where alternatives herbicides (2,4-D, glyphosate, glufosinate, flazasulfuron and pyraflufen-ethyl) with different MOAs were used, but they have also showed failure in controlling this species...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29892181/diagnostic-value-of-glypican3-heat-shock-protein-70-and-glutamine-synthetase-in-hepatocellular-carcinoma-arising-in-cirrhotic-and-non-cirrhotic-livers
#17
Preithy Uthamalingam, Ashim Das, Arunanshu Behra, Naveen Kalra, Yogesh Chawla
Background and objectives: Histopathological distinction of various nodular lesions in liver with sufficient sensitivity and specificity is a challenge even in an expert set up. The panel of immunohistochemical markers composed of glutamine synthetase (GS), Glypican3 (GPC3) and heat shock protein 70 (HSP70) was recommended by the International Consensus Group for Hepatocellular Neoplasia group for the differentiation of high grade dysplastic nodule and early hepatocellular carcinoma (HCC)...
June 2018: Journal of Clinical and Experimental Hepatology
https://www.readbyqxmd.com/read/29891741/heterologous-expression-of-key-c-and-n-metabolic-enzymes-improves-re-assimilation-of-photorespired-co2-and-nh3-and-growth
#18
Anish Kaachra, Surender Kumar Vats, Sanjay Kumar
We investigated the effect of heterologous expression of phosphoenolpyruvate carboxylase (ZmPepcase), aspartate aminotransferase (GmAspAT), and glutamine synthetase (NtGS) on carbon (C) and nitrogen (N) metabolism in Arabidopsis (Arabidopsis thaliana). These transgenes were expressed either separately or in different combinations. The highest gains in shoot dry weight were observed in transgenic lines co-expressing all three genes. Tracer experiments using NaH14CO3 suggested that the co-expression of ZmPepcase, GmAspAT,and NtGS resulted in a higher flux of assimilated CO2 towards sugars and amino acids...
June 11, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29891377/implication-of-fusarium-graminearum-primary-metabolism-in-its-resistance-to-benzimidazole-fungicides-as-revealed-by-1-h-nmr-metabolomics
#19
A Sevastos, I F Kalampokis, A Panagiotopoulou, M Pelecanou, K A Aliferis
Fungal metabolomics is a field of high potential but yet largely unexploited. Focusing on plant-pathogenic fungi, no metabolomics studies exist on their resistance to fungicides, which represents a major issue that the agrochemical and agricultural sectors are facing. Fungal infections cause quantitative, but also qualitative yield losses, especially in the case of mycotoxin-producing species. The aim of the study was to correlate metabolic changes in Fusarium graminearum strains' metabolomes with their carbendazim-resistant level and discover corresponding metabolites-biomarkers, with primary focus on its primary metabolism...
June 2018: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29890290/the-selective-glucocorticoid-receptor-modulator-cort-113176-reduces-neurodegeneration-and-neuroinflammation-in-wobbler-mice-spinal-cord
#20
Maria Meyer, Agustina Lara, Hazel Hunt, Joseph Belanoff, E Ronald de Kloet, Maria Claudia Gonzalez Deniselle, Alejandro F De Nicola
Wobbler mice are experimental models for amyotrophic lateral sclerosis. As such they show motoneuron degeneration, motor deficits, and astrogliosis and microgliosis of the spinal cord. Additionally, Wobbler mice show increased plasma, spinal cord and brain corticosterone levels and focal adrenocortical hyperplasia, suggesting a pathogenic role for glucocorticoids in this disorder. Considering this endocrine background, we examined whether the glucocorticoid receptor (GR) modulator CORT 113176 prevents spinal cord neuropathology of Wobblers...
June 8, 2018: Neuroscience
keyword
keyword
10008
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"