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https://www.readbyqxmd.com/read/28741788/molecular-analysis-of-single-circulating-tumour-cells-following-long-term-storage-of-clinical-samples
#1
Barbara Mesquita, Dominic G Rothwell, Deborah J Burt, Francesca Chemi, Fabiola Fernandez-Gutierrez, Daniel Slane-Tan, Jenny Antonello, Mathew Carter, Louise Carter, Marina Parry, Lynsey Franklin, Richard Marais, Fiona Blackhall, Caroline Dive, Ged Brady
The CellSearch® semi-automated CTC enrichment and staining system has been established as the "gold standard" for CTC enumeration with CellSearch® CTC counts recognized by the FDA as prognostic for a number of cancers. We and others have gone on to show that molecular analysis of CellSearch® CTCs isolated shortly after CellSearch® enrichment provides another valuable layer of information that has potential clinical utility including predicting response to treatment. Although CellSearch® CTCs can be readily isolated after enrichment, the process of analysing a single CellSearch® patient sample, which may contain many CTCs, is both time consuming and costly...
July 25, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28741083/construction-of-bioengineered-yeast-platform-for-direct-bioethanol-production-from-alginate-and-mannitol
#2
Toshiyuki Takagi, Yusuke Sasaki, Keisuke Motone, Toshiyuki Shibata, Reiji Tanaka, Hideo Miyake, Tetsushi Mori, Kouichi Kuroda, Mitsuyoshi Ueda
Brown macroalgae are a sustainable and promising source for bioethanol production because they are abundant in ocean ecosystems and contain negligible quantities of lignin. Brown macroalgae contain cellulose, hemicellulose, mannitol, laminarin, and alginate as major carbohydrates. Among these carbohydrates, brown macroalgae are characterized by high levels of alginate and mannitol. The direct bioconversion of alginate and mannitol into ethanol requires extensive bioengineering of assimilation processes in the standard industrial microbe Saccharomyces cerevisiae...
July 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28736156/improving-the-economics-of-nash-nafld-treatment-through-the-use-of-systems-biology
#3
REVIEW
Jim Bosley, Christofer Boren, Sunjae Lee, Morten Grøtli, Jens Nielsen, Mathias Uhlen, Jan Boren, Adil Mardinoglu
Nonalcoholic steatohepatitis (NASH) is a severe form of nonalcoholic fatty liver disease (NAFLD). We surveyed NASH therapies currently in development, and found a significant variety of targets and approaches. Evaluation and clinical testing of these targets is an expensive and time-consuming process. Systems biology approaches could enable the quantitative evaluation of the likely efficacy and safety of different targets. This motivated our review of recent systems biology studies that focus on the identification of targets and development of effective treatments for NASH...
July 20, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28735394/detection-of-differential-dna-methylation-under-stress-conditions-using-bisulfite-sequence-analysis
#4
Ibtisam Al Harrasi, Rashid Al-Yahyai, Mahmoud W Yaish
DNA methylation is the most important epigenetic change affecting gene expression in plants grown under normal as well as under stress conditions. Therefore, researchers study differential DNA methylation under distinct environmental conditions and their relationship with transcriptome abundance. Up to date, more than 25 methods and techniques are available to detect DNA methylation based on different principles. Bisulfite sequencing method is considered as a gold standard since it is able to distinguish 5-methylcytosine from cytosine using the bisulfite treatment...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28727022/triennial-growth-and-development-symposium-genetics-and-breeding-for-intramuscular-fat-and-oleic-acid-content-in-pigs
#5
J Estany, R Ros-Freixedes, M Tor, R N Pena
The intramuscular fat (IMF) and oleic acid (OL) content have been favorably related to pork quality and human health. This influences the purchasing behavior of consumers and, therefore, also shifts the attention of breeding companies toward whether these traits are included into the breeding goal of the lines producing for high-valued markets. Because IMF and OL are unfavorably associated with lean content, a key economic trait, the real challenge for the industry is not simply to increase IMF and OL, but rather to come up with the right trade-off between them and lean content...
May 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28716969/genetic-determinants-of-susceptibility-to-silver-nanoparticle-induced-acute-lung-inflammation-in-mice
#6
David K Scoville, Dianne Botta, Karen Galdanes, Stefanie C Schmuck, Collin C White, Patricia L Stapleton, Theo K Bammler, James W MacDonald, William A Altemeier, Michelle Hernandez, Steven R Kleeberger, Lung-Chi Chen, Terry Gordon, Terrance J Kavanagh
Silver nanoparticles (AgNPs) are employed in a variety of consumer products; however, in vivo rodent studies indicate that AgNPs can cause lung inflammation and toxicity in a strain- and particle type-dependent manner, but mechanisms of susceptibility remain unclear. The aim of this study was to assess the variation in AgNP-induced lung inflammation and toxicity across multiple inbred mouse strains and to use genome-wide association (GWA) mapping to identify potential candidate susceptibility genes. Mice received doses of 0...
July 17, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28716627/membrane-proteins-structures-a-review-on-computational-modeling-tools
#7
REVIEW
Jose G Almeida, Antonio J Preto, Panagiotis I Koukos, Alexandre M J J Bonvin, Irina S Moreira
BACKGROUND: Membrane proteins (MPs) play diverse and important functions in living organisms. They constitute 20% to 30% of the known bacterial, archaean and eukaryotic organisms' genomes. In humans, their importance is emphasized as they represent 50% of all known drug targets. Nevertheless, experimental determination of their three-dimensional (3D) structure has proven to be both time consuming and rather expensive, which has led to the development of computational algorithms to complement the available experimental methods and provide valuable insights...
July 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28713402/unmanned-aerial-vehicle-remote-sensing-for-field-based-crop-phenotyping-current-status-and-perspectives
#8
REVIEW
Guijun Yang, Jiangang Liu, Chunjiang Zhao, Zhenhong Li, Yanbo Huang, Haiyang Yu, Bo Xu, Xiaodong Yang, Dongmei Zhu, Xiaoyan Zhang, Ruyang Zhang, Haikuan Feng, Xiaoqing Zhao, Zhenhai Li, Heli Li, Hao Yang
Phenotyping plays an important role in crop science research; the accurate and rapid acquisition of phenotypic information of plants or cells in different environments is helpful for exploring the inheritance and expression patterns of the genome to determine the association of genomic and phenotypic information to increase the crop yield. Traditional methods for acquiring crop traits, such as plant height, leaf color, leaf area index (LAI), chlorophyll content, biomass and yield, rely on manual sampling, which is time-consuming and laborious...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28712292/crystal-structure-of-cocosin-a-potential-food-allergen-from-coconut-cocos-nucifera
#9
Tengchuan Jin, Cheng Wang, Caiying Zhang, Yang Wang, Yu-Wei Chen, Feng Guo, Andrew Howard, Min-Jie Cao, Tong-Jen Fu, Tara H McHugh, Yuzhu Zhang
Coconut (Cocos nucifera) is an important palm tree. Coconut fruit is widely consumed. The most abundant storage protein in coconut fruit is cocosin (a likely food allergen), which belongs to the 11S globulin family. Cocosin was crystallized near a century ago, but its structure remains unknown. By optimizing crystallization conditions and cryoprotectant solutions, we were able to obtain cocosin crystals that diffracted to 1.85 Å. The cocosin gene was cloned from genomic DNA isolated from dry coconut tissue...
July 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28711269/development-of-an-isothermal-amplification-based-assay-for-the-rapid-visual-detection-of-salmonella-bacteria
#10
Hai-Bin Liu, Yu-Xuan Zang, Xin-Jun Du, Ping Li, Shuo Wang
The efficient and timely detection of pathogens is a major concern worldwide. The aim of this study was to establish a rapid detection method for Salmonella bacteria in food samples to facilitate timely treatment. Widely used detection methods currently include culture-based methods and PCR-based methods. The former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. In this study, a sensitive and rapid approach for the visual and point-of-use detection of Salmonella bacteria based on recombinase polymerase amplification (RPA) and a lateral-flow (LF) nucleic acid strip was established...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28702933/characterization-of-simbu-serogroup-virus-infections-in-type-i-interferon-receptor-knock-out-mice
#11
Kerstin Tauscher, Kerstin Wernike, Melina Fischer, Anne Wegelt, Bernd Hoffmann, Jens Peter Teifke, Martin Beer
In late 2011, Schmallenberg virus (SBV), a novel, arthropod-borne, teratogenic orthobunyavirus, emerged near the German/Dutch border and thereafter spread rapidly throughout the continent thereby causing great economic losses in European livestock. SBV mainly infects ruminants and closely related viruses such as Sabo virus (SABOV), Simbu virus (SIMBUV) and Sathuperi virus (SATV) have been isolated from their insect-vectors or putative ruminant hosts. However, information about their pathogenesis and in vivo studies with SABOV, SIMBUV, and SATV are scarce...
July 12, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28696026/a-genome-wide-association-study-for-a-proxy-of-intermuscular-fat-level-in-the-italian-large-white-breed-identifies-genomic-regions-affecting-an-important-quality-parameter-for-dry-cured-hams
#12
L Fontanesi, G Schiavo, G Galimberti, S Bovo, V Russo, M Gallo, L Buttazzoni
Intermuscular fat content in protected designations of origin dry-cured hams is a very important meat quality trait that affects the acceptability of the product by the consumers. An excess in intermuscular fat (defined as the level of fat deposition between leg muscles) is a defect that depreciates the final product. In this study we carried out a genome-wide association study for visible intermuscular fat (VIF) of hams in the Italian Large White pig breed. This trait was evaluated on the exposed muscles of green legs in 1122 performance-tested gilts by trained personnel, according to a classification scale useful for routine and cheap evaluation...
August 2017: Animal Genetics
https://www.readbyqxmd.com/read/28694792/context-is-everything-harmonization-of-critical-food-microbiology-descriptors-and-metadata-for-improved-food-safety-and-surveillance
#13
Emma Griffiths, Damion Dooley, Morag Graham, Gary Van Domselaar, Fiona S L Brinkman, William W L Hsiao
Globalization of food networks increases opportunities for the spread of foodborne pathogens beyond borders and jurisdictions. High resolution whole-genome sequencing (WGS) subtyping of pathogens promises to vastly improve our ability to track and control foodborne disease, but to do so it must be combined with epidemiological, clinical, laboratory and other health care data (called "contextual data") to be meaningfully interpreted for regulatory and health interventions, outbreak investigation, and risk assessment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28694404/-the-role-of-omics-technology-in-the-pathophysiology-diagnosis-and-treatment-of-cardiovascular-diseases
#14
Ümit Yaşar Sinan, Serkan Unlu
Although evident improvement has occurred in the diagnosis and treatment of cardiovascular disease (CVD), it is still the most important cause of mortality worldwide. The majority of the CVDs are multifactorial and polygenic. Therefore, it is logical to use genomics, proteomics, lipidomics, and metabolomics together for diagnosis and effective treatment of CVD. "Genome" is the combination of the words "gene" and "chromosome," and includes all protein-coding genes and intergenic spaces (as well as intragenic regions, or introns, within genes) in an organism...
July 2017: Türk Kardiyoloji Derneği Arşivi: Türk Kardiyoloji Derneğinin Yayın Organıdır
https://www.readbyqxmd.com/read/28683768/extrapolative-microrna-precursor-based-ssr-mining-from-tea-est-database-in-respect-to-agronomic-traits
#15
Anjan Hazra, Nirjhar Dasgupta, Chandan Sengupta, Sauren Das
Tea (Camellia sinensis, (L.) Kuntze) is considered as most popular drink across the world and it is widely consumed beverage for its several health-benefit characteristics. These positive traits primarily rely on its regulatory networks of different metabolic pathways. Development of microsatellite markers from the conserved genomic regions are being worthwhile for reviewing the genetic diversity of closely related species or self-pollinated species. Although several SSR markers have been reported, in tea, the trait-specific Simple Sequence Repeat (SSR) markers, leading to be useful in marker assisted breeding technique, are yet to be identified...
July 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28674382/the-destiny-of-the-resistance-susceptibility-against-gcrv-is-controlled-by-epigenetic-mechanisms-in-cik-cells
#16
Xueying Shang, Chunrong Yang, Quanyuan Wan, Youliang Rao, Jianguo Su
Hemorrhagic disease caused by grass carp reovirus (GCRV) has severely threatened the grass carp (Ctenopharyngodon idella) cultivation industry. It is noteworthy that the resistance against GCRV infection was reported to be inheritable, and identified at both individual and cellular levels. Therefore, this work was inspired and dedicated to unravel the molecular mechanisms of fate decision post GCRV infection in related immune cells. Foremost, the resistant and susceptible CIK (C. idella kidney) monoclonal cells were established by single cell sorting, subculturing and infection screening successively...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28668351/utility-of-crispr-cas9-systems-in-hematology-research
#17
REVIEW
Daniel Lucas, Heather A O'Leary, Benjamin L Ebert, Chad A Cowan, Cedric S Tremblay
Since the end of the 20th century, novel approaches have emerged to manipulate experimental models of hematological disorders so that they more accurately mirror what is observed in the clinical setting. Despite these technological advances, the characterization of crucial genes for benign or malignant hematological disorders remains challenging, given the dynamic nature of the hematopoietic system and the genetic heterogeneity of these disorders. To overcome this limitation, genome-editing technologies have been developed to manipulate the genome specifically via deletion, insertion, or modification of targeted loci...
June 28, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/28668117/expression-and-structural-analyses-of-human-dna-polymerase-%C3%AE-polq
#18
Andrew W Malaby, Sara K Martin, Richard D Wood, Sylvie Doublié
DNA polymerase theta (pol θ) is an evolutionarily conserved protein encoded by the POLQ gene in mammalian genomes. Pol θ is the defining enzyme for a pathway of DSB repair termed "alternative end-joining" (altEJ) or "theta-mediated end-joining." This pathway contributes significantly to the radiation resistance of mammalian cells. It also modulates accuracy in repair of breaks that occur at stalled DNA replication forks, during diversification steps of the mammalian immune system, during repair of CRISPR-Cas9, and in many DNA integration events...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28667254/quantity-of-alcohol-drinking-positively-correlates-with-serum-levels-of-endotoxin-and-markers-of-monocyte-activation
#19
Suthat Liangpunsakul, Evelyn Toh, Ruth A Ross, Laura E Heathers, Kristina Chandler, AdePeju Oshodi, Breann McGee, Elizabeth Modlik, Tobyn Linton, Darrin Mangiacarne, Claudie Jimenez, X Charlie Dong, Li Wang, Wanzhu Tu, David E Nelson
It is unknown if LPS (lipopolysaccharides) and markers of immune activation, soluble CD14 (sCD14) and CD163 (sCD163) are associated with the quantity of alcohol consumption. 148 subjects were enrolled (97 excessive drinkers (ED) and 51 controls). Time Line Follow-Back questionnaire was used to quantify the amount of alcohol consumed. Serum LPS, sCD14, and sCD163 were measured. Peripheral blood mononuclear cells (PBMCs) were also isolated. Compared to controls, ED had higher total drinks in the past 30 days, higher levels of LPS, sCD14 and sCD163...
June 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28666889/efficient-genome-editing-by-crispr-cas9-with-a-trna-sgrna-fusion-in-the-methylotrophic-yeast-ogataea-polymorpha
#20
Minori Numamoto, Hiromi Maekawa, Yoshinobu Kaneko
The methylotrophic yeast Ogataea polymorpha (syn. Hansenula polymorpha) is an attractive industrial non-conventional yeast showing high thermo-tolerance (up to 50°C) and xylose assimilation. However, genetic manipulation of O. polymorpha is often laborious and time-consuming because it has lower homologous recombination efficiency relative to Saccharomyces cerevisiae. To overcome this disadvantage, we applied the CRISPR/Cas9 system as a powerful genome editing tool in O. polymorpha. In this system, both single guide RNA (sgRNA) and endonuclease Cas9 were expressed by a single autonomously-replicable plasmid and the sgRNA portion could be easily changed by using PCR and In-Fusion cloning techniques...
June 27, 2017: Journal of Bioscience and Bioengineering
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