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https://www.readbyqxmd.com/read/28921810/bones-morphogenic-protein-4-and-retinoic-acid-combined-treatment-comparative-analysis-for-in-vitro-differentiation-potential-of-murine-mesenchymal-stem-cells-derived-from-bone-marrow-and-adipose-tissue-into-germ-cells
#1
Maryam H Shirzeily, Neda Khanlarkhani, Fardin Amidi, Farshad H Shirzeily, Fereydoon S Aval, Aligholi Sobhani
Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to investigate the efficacy of a combined medium, retinoic acid (RA) together with Bone Morphogenic Protein-4 (BMP4), on differentiation of Bone Marrow Mesenchymal Stem Cells (BMMSCs) and adipose-derived mesenchymal stem cells (ADMSCs) into germ cells...
September 18, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28916299/non-canonical-wnt-mediated-neurogenic-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells
#2
Sujeong Jang, Hyong-Ho Cho, Jong-Seong Park, Han-Seong Jeong
Bone marrow-derived mesenchymal stem cells (BM-MSCs), which are characterized by multipotency and self-renewal, are responsible for tissue regeneration and repair. We have previously reported in adipose tissue-derived MSCs that only Wnt5a is enhanced at neurogenic differentiation, and the mechanism of differentiation is dependent on the Wnt5a/JNK pathway; however, the role of Wnt/MAPK pathway is yet to be investigated in neurogenic differentiation in BM-MSCs. We compared the transcriptional expression of Wnt in neurogenic induced-hBM-MSCs (NI-hBM-MSCs) with that in primary hBM-MSCs, using RT-PCR, qPCR, and western blotting...
September 12, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28910187/sternal-route-more-effective-than-tibial-route-for-intraosseous-amiodarone-administration-in-a-swine-model-of-ventricular-fibrillation
#3
James M Burgert, Andre Martinez, Mara O'Sullivan, Dawn Blouin, Audrey Long, Arthur D Johnson
OBJECTIVE: The pharmacokinetics of IO administered lipid soluble amiodarone during ventricular fibrillation (VF) with ongoing CPR are unknown. This study measured mean plasma concentration over 5 minutes, maximum plasma concentration (Cmax), and time to maximum concentration (Tmax) of amiodarone administered by the sternal IO (SIO), tibial IO (TIO), and IV routes in a swine model of VF with ongoing CPR. METHODS: Twenty-one Yorkshire-cross swine were randomly assigned to three groups: SIO, TIO, and IV...
September 14, 2017: Prehospital Emergency Care
https://www.readbyqxmd.com/read/28902422/transplantation-of-non-cultured-stromal-vascular-fraction-cells-of-adipose-tissue-ameliorates-osteonecrosis-of-the-jaw-like-lesions-in-mice
#4
Shinichiro Kuroshima, Muneteru Sasaki, Kazunori Nakajima, Saki Tamaki, Hiroki Hayano, Takashi Sawase
The precise pathoetiology and effective treatment strategies for bisphosphonate-related osteonecrosis of the jaw (BRONJ) remain unknown. Transplantation of non-cultured stromal vascular fraction (SVF) cells has been demonstrated to be a useful method for regenerative medicine in place of stem cell therapy. This study investigated the effects of non-cultured SVF transplantation on tooth extraction socket healing in mice. Both chemotherapeutic/bisphosphonate combination therapy for 7 weeks and tooth extraction of maxillary first molars at 3 weeks after drug administration were performed using female C57BL/6J mice...
September 13, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28894288/altered-adipose-derived-stem-cell-characteristics-in-macrodactyly
#5
Xi Yang, Yongkang Jiang, Gang Han, Yuan Shi, Shengbo Zhou, Feng Ni, Bin Wang
Macrodactyly is a congenital disease characterized by aggressive overgrowth of adipose tissue in digits or limbs frequently accompanied with hyperostosis and nerve enlargement; its pathological mechanism is poorly understood. Adipose-derived stem cells (ASCs) have been extensively studied in tissue engineering and regenerative medicine as an ideal alternative substitute for bone marrow-derived mesenchymal stem cells (BM-MSCs), but their pathological role is largely unknown. In this study, ASCs from macrodactyly adipose tissues (Mac-ASCs) were isolated and compared to ASCs derived from the normal abdominal subcutaneous adipose tissue (Sat-ASCs) for cell morphology, surface marker expression, proliferation rate, and tri-lineage differentiation potential...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28885289/mesenchymal-stem-cell-levels-of-human-spinal-tissues
#6
Liam Harris, C Thomas Vangsness
STUDY DESIGN: .: Systematic Review. OBJECTIVE: .: The aim of this study was to investigate, quantify, compare and compile the various mesenchymal stem cell tissue sources within human spinal tissues to act as a compendium for clinical and research application. SUMMARY OF BACKGROUND DATA: .: Recent years have seen a dramatic increase in academic and clinical understanding of human mesenchymal stem cells (MSCs). Previously limited to cells isolated from bone marrow, the past decade has illicited the characterization and isolation of human MSCs from adipose, bone marrow, synovium, muscle, periosteum, peripheral blood, umbilical cord, placenta and numerous other tissues...
September 6, 2017: Spine
https://www.readbyqxmd.com/read/28874054/critical-view-on-mesenchymal-stromal-cells-in-regenerative-medicine
#7
Agnieszka Langrzyk, Witold Norbert Nowak, Jacek Stepniewski, Agnieszka Jazwa, Urszula Florczyk, Alicja Jozkowicz, Jozef Dulak
The belief in the potency of stem cells has resulted in the medical applications of numerous cell types for organ repair, often with the low adherence to methodological stringency. Such uncritical enthusiasm is mainly presented in the approaches employing so-called mesenchymal stem cells (MSC), originally isolated from the bone marrow, then from other sources like adipose tissue or placenta, which are used for the treatment of numerous, unrelated condition. However, it should be stressed that such clinical applications of MSC were based on unsubstantiated belief that MSC can efficiently differentiate into multiple cell types, not only osteoblasts, chondrocytes and adipose cells...
September 6, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28872979/bone-marrow-adipocytes
#8
Mark C Horowitz, Ryan Berry, Brandon Holtrup, Zachary Sebo, Tracy Nelson, Jackie A Fretz, Dieter Lindskog, Jennifer L Kaplan, Gene Ables, Matthew S Rodeheffer, Clifford J Rosen
Adipocytes were identified in human bone marrow more than a century ago, yet until recently little has been known about their origin, development, function or interactions with other cells in the bone marrow. Little functional significance has been attributed to these cells, a paradigm that still persists today. However, we now know that marrow adipose tissue increases with age and in response to a variety of physiologic induction signals. Bone marrow adipocytes have recently been shown to influence other cell populations within the marrow and can affect whole body metabolism by the secretion of a defined set of adipokines...
August 24, 2017: Adipocyte
https://www.readbyqxmd.com/read/28871128/synergistic-effect-of-bdnf-and-fgf2-in-efficient-generation-of-functional-dopaminergic-neurons-from-human-mesenchymal-stem-cells
#9
Manisha Singh, Anupama Kakkar, Rinkey Sharma, O P Kharbanda, Nitika Monga, Manish Kumar, Shantanu Chowdhary, Balram Airan, Sujata Mohanty
To understand the process of neurogenesis, generation of functional dopaminergic (DAergic) neurons from human mesenchymal stem cells (hMSCs) is important. BDNF has been reported to be responsible for inducing neuronal maturation and functionality. Previously, we have reported the efficient generation of neurons from human bone marrow derived MSCs using FGF2 alone. We hypothesize that hMSCs from various tissues [(bone marrow (BM), adipose tissue (AD) and dental pulp (DP)], if treated with BDNF on 9(th) day of induction, alongwith FGF2 will generate functional DAergic neurons...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28868898/the-tumour-suppressor-cdkn2a-p16-ink4a-regulates-adipogenesis-and-bone-marrow-dependent-development-of-perivascular-adipose-tissue
#10
Kristiaan Wouters, Yann Deleye, Sarah A Hannou, Jonathan Vanhoutte, Xavier Maréchal, Augustin Coisne, Madjid Tagzirt, Bruno Derudas, Emmanuel Bouchaert, Christian Duhem, Emmanuelle Vallez, Casper G Schalkwijk, François Pattou, David Montaigne, Bart Staels, Réjane Paumelle
The genomic CDKN2A/B locus, encoding p16(INK4a) among others, is linked to an increased risk for cardiovascular disease and type 2 diabetes. Obesity is a risk factor for both cardiovascular disease and type 2 diabetes. p16(INK4a) is a cell cycle regulator and tumour suppressor. Whether it plays a role in adipose tissue formation is unknown. p16(INK4a) knock-down in 3T3/L1 preadipocytes or p16(INK4a) deficiency in mouse embryonic fibroblasts enhanced adipogenesis, suggesting a role for p16(INK4a) in adipose tissue formation...
September 1, 2017: Diabetes & Vascular Disease Research
https://www.readbyqxmd.com/read/28860152/proteasome-inhibitor-bortezomib-is-a-novel-therapeutic-agent-for-focal-radiation-induced-osteoporosis
#11
Abhishek Chandra, Luqiang Wang, Tiffany Young, Leilei Zhong, Wei-Ju Tseng, Michael A Levine, Keith Cengel, X Sherry Liu, Yejia Zhang, Robert J Pignolo, Ling Qin
Bone atrophy and its related fragility fractures are frequent, late side effects of radiotherapy in cancer survivors and have a detrimental impact on their quality of life. In another study, we showed that parathyroid hormone 1-34 and anti-sclerostin antibody attenuates radiation-induced bone damage by accelerating DNA repair in osteoblasts. DNA damage responses are partially regulated by the ubiquitin proteasome pathway. In the current study, we examined whether proteasome inhibitors have similar bone-protective effects against radiation damage...
August 31, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28855315/eplerenone-prevented-obesity-induced-inflammasome-activation-and-glucose-intolerance
#12
Tsutomu Wada, Akari Ishikawa, Eri Watanabe, Yuto Nakamura, Yusuke Aruga, Hayate Hasegawa, Yasuhiro Onogi, Hiroe Honda, Yoshinori Nagai, Kiyoshi Takatsu, Yoko Ishii, Masakiyo Sasahara, Daisuke Koya, Hiroshi Tsuneki, Toshiyasu Sasaoka
Obesity-associated activation of the renin-angiotensin-aldosterone system is implicated in the pathogenesis of insulin resistance; however, influences of mineralocorticoid receptor (MR) inhibition remain unclear. Therefore, we aimed to clarify the anti-inflammatory mechanisms of MR inhibition using eplerenone, a selective MR antagonist, in C57BL/6 mice fed a high-fat diet (HFD) for 12 weeks. Eplerenone prevented excessive body weight gain and fat accumulation, ameliorated glucose intolerance and insulin resistance, and enhanced energy metabolism...
August 30, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28852131/raman-spectroscopy-uncovers-biochemical-tissue-related-features-of-extracellular-vesicles-from-mesenchymal-stromal-cells
#13
Alice Gualerzi, Stefania Niada, Chiara Giannasi, Silvia Picciolini, Carlo Morasso, Renzo Vanna, Valeria Rossella, Massimo Masserini, Marzia Bedoni, Fabio Ciceri, Maria Ester Bernardo, Anna Teresa Brini, Furio Gramatica
Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28844130/proteomic-analysis-of-human-adipose-derived-stem-cells-during-small-molecule-chemical-stimulated-pre-neuronal-differentiation
#14
Jerran Santos, Bruce K Milthorpe, Benjamin R Herbert, Matthew P Padula
Background: Adipose derived stem cells (ADSCs) are acquired from abdominal liposuction yielding a thousand fold more stem cells per millilitre than those from bone marrow. A large research void exists as to whether ADSCs are capable of transdermal differentiation toward neuronal phenotypes. Previous studies have investigated the use of chemical cocktails with varying inconclusive results. Methods: Human ADSCs were treated with a chemical stimulant, beta-mercaptoethanol, to direct them toward a neuronal-like lineage within 24 hours...
August 31, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28843066/deriving-vascular-smooth-muscle-cells-from-mesenchymal-stromal-cells-evolving-differentiation-strategies-and-current-understanding-of-their-mechanisms
#15
REVIEW
Xiaoqing Zhang, Michelle P Bendeck, Craig A Simmons, J Paul Santerre
Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function. Regeneration of functional vascular smooth muscle tissue to repair vascular diseases is an area of intense research in tissue engineering and regenerative medicine. For functional vascular smooth muscle tissue regeneration to become a practical therapy over the next decade, the field will need to have access to VSMC sources that are effective, robust and safe. While pluripotent stem cells hold good future promise to this end, more immediate translation is expected to come from approaches that generate functional VSMCs from adult sources of multipotent adipose-derived and bone marrow-derived mesenchymal stromal cells (ASCs and BMSCs)...
August 15, 2017: Biomaterials
https://www.readbyqxmd.com/read/28841158/mesenchymal-stem-cell-secretome-toward-cell-free-therapeutic-strategies-in-regenerative-medicine
#16
REVIEW
Francisco J Vizoso, Noemi Eiro, Sandra Cid, Jose Schneider, Roman Perez-Fernandez
Earlier research primarily attributed the effects of mesenchymal stem cell (MSC) therapies to their capacity for local engrafting and differentiating into multiple tissue types. However, recent studies have revealed that implanted cells do not survive for long, and that the benefits of MSC therapy could be due to the vast array of bioactive factors they produce, which play an important role in the regulation of key biologic processes. Secretome derivatives, such as conditioned media or exosomes, may present considerable advantages over cells for manufacturing, storage, handling, product shelf life and their potential as a ready-to-go biologic product...
August 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28833807/the-wnt5a-receptor-ror2-is-a-predictive-cell-surface-marker-of-human-mesenchymal-stem-cells-with-an-enhanced-capacity-for-chondrogenic-differentiation
#17
Sally C Dickinson, Catherine A Sutton, Kyla Brady, Anna Salerno, Theoni Katopodi, Rhys L Williams, Christopher C West, Denis Evseenko, Ling Wu, Suzanna Pang, Roberta Ferro de Godoy, Allen E Goodship, Bruno Péault, Ashley W Blom, Wael Kafienah, Anthony P Hollander
Multipotent mesenchymal stem cells (MSCs) have enormous potential in tissue engineering and regenerative medicine. However until now their development for clinical use has been severely limited as they are a mixed population of cells with varying capacities for lineage differentiation and tissue formation. Here we identify ROR2 as a cell surface marker expressed by those MSCs with an enhanced capacity for cartilage formation. We generated clonal human MSC populations with varying capacities for chondrogenesis...
August 20, 2017: Stem Cells
https://www.readbyqxmd.com/read/28821996/mesenchymal-stromal-stem-cell-separation-methods-concise-review
#18
REVIEW
Andreas Nicodemou, Lubos Danisovic
Mesenchymal stem (stromal) cells (MSCs) possess unique biological characteristics such as plasticity, long term self-renewal, secretion of various bioactive molecules and ability of active migration to the diseased tissues that make them unique tool for regenerative medicine, nowadays. Until now MSCs were successfully derived from many tissue sources including bone marrow, umbilical cord, adipose tissue, dental pulp etc. The crucial step prior to their in vitro expansion, banking or potential clinical application is their separation...
August 18, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28821457/spontaneous-mutation-of-dock7-results-in-lower-trabecular-bone-mass-and-impaired-periosteal-expansion-in-aged-female-misty-mice
#19
Phuong T Le, Kathleen A Bishop, David E Maridas, Katherine J Motyl, Daniel J Brooks, Kenichi Nagano, Roland Baron, Mary L Bouxsein, Clifford J Rosen
Misty mice (m/m) have a loss of function mutation in Dock7 gene, a guanine nucleotide exchange factor, resulting in low bone mineral density, uncoupled bone remodeling and reduced bone formation. Dock7 has been identified as a modulator of osteoblast number and in vitro osteogenic differentiation in calvarial osteoblast culture. In addition, m/m exhibit reduced preformed brown adipose tissue innervation and temperature as well as compensatory increase in beige adipocyte markers. While the low bone mineral density phenotype is in part due to higher sympathetic nervous system (SNS) drive in young mice, it is unclear what effect aging would have in mice homozygous for the mutation in the Dock7 gene...
August 15, 2017: Bone
https://www.readbyqxmd.com/read/28818979/incidental-brown-adipose-tissue-in-bone-marrow-biopsy
#20
Jennifer Chapman, Francisco Vega
No abstract text is available yet for this article.
August 17, 2017: Blood
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