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Journal of Circulating Biomarkers

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https://www.readbyqxmd.com/read/29662552/strategies-for-enumeration-of-circulating-microvesicles-on-a-conventional-flow-cytometer-counting-beads-and-scatter-parameters
#1
Mohammad J Alkhatatbeh, Anoop K Enjeti, Sara Baqar, Elif I Ekinci, Dorothy Liu, Rick F Thorne, Lisa F Lincz
Enumeration of circulating microvesicles (MVs) by conventional flow cytometry is accomplished by the addition of a known amount of counting beads and calculated from the formula: MV/μl = (MV count/bead count) × final bead concentration. We sought to optimize each variable in the equation by determining the best parameters for detecting 'MV count' and examining the effects of different bead preparations and concentrations on the final calculation. Three commercially available bead preparations (TruCount, Flow-Count and CountBright) were tested, and MV detection on a BD FACSCanto was optimized for gating by either forward scatter (FSC) or side scatter (SSC); the results were compared by calculating different subsets of MV on a series of 74 typical patient plasma samples...
January 2018: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/29623111/case-report-whole-exome-sequencing-of-circulating-cell-free-tumor-dna-in-a-follicular-thyroid-carcinoma-patient-with-lung-and-bone-metastases
#2
Jianlu Song, Zhili Yang
Metastatic follicular thyroid carcinoma (FTC), unresectable or resistance to radioactive iodine, is associated with poor survival. It is believed that this kind of FTC is driven by mutated genes. However, what kind of changes of genome and underlying mechanisms are elusive. The aim of this article is to understand whether there are somatic mutations in circulating cell-free tumor DNA (cfDNA) in a FTC patient with lung and bone metastases. A 55-year-old woman was diagnosed with FTC with bone and lung metastases...
January 2018: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/29375722/correlations-of-gdf-15-with-sst2-mmps-and-worsening-functional-capacity-in-idiopathic-dilated-cardiomyopathy-can-we-gain-new-insights-into-the-pathophysiology
#3
Nandini Nair, Enrique Gongora
Growth and differentiation factor-15 (GDF-15) has been implicated in fibrosis, inflammation, and ventricular remodeling. The role of GDF-15 in the regulation of cardiac remodeling in idiopathic dilated cardiomyopathy (DCM) remains poorly defined. This study attempts to analyze the molecular interactions between GDF-15 and markers of fibrosis as well as its positive correlations with worsening functional capacity. The study population consisted of 24 DCM patients and 8 control subjects. All DCM patients had normal coronary angiographic studies...
January 2018: Journal of circulating biomarkers
https://www.readbyqxmd.com/read/29270236/-journal-of-circulating-biomarkers-looks-forward-after-being-indexed-in-pubmed-central
#4
EDITORIAL
Winston Patrick Kuo
No abstract text is available yet for this article.
January 2017: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/29081845/the-effects-of-aerobic-and-anaerobic-exercises-on-circulating-soluble-klotho-and-igf-i-in-young-and-elderly-adults-and-in-cad-patients
#5
REVIEW
Moran S Saghiv, D Ben Sira, E Goldhammer, M Sagiv
Different studies support the notion that chronic aerobic exercises training can influence the circulating levels of soluble-Klotho (s-Klotho) and insulin-like growth factor 1 (IGF-I). The effects of s-Klotho include improving the quality of life, alleviating the negative impact of age on the body's work capacity, and possibly increasing longevity. This review provides an overview of the latest findings in this field of research in humans. The different modes of dynamic exercise and their impact on circulating levels of s-Klotho and IGF-I in young adult athletes, untrained young adults, trained healthy older adults, untrained healthy older adults, and coronary artery disease (CAD) patients are reviewed and discussed...
January 2017: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936268/mildly-elevated-serum-total-bilirubin-is-negatively-associated-with-hemoglobin-a1c-independently-of-confounding-factors-among-community-dwelling-middle-aged-and-elderly-persons
#6
Ryuichi Kawamoto, Daisuke Ninomiya, Kensuke Senzaki, Teru Kumagi
Abnormally high glycated hemoglobin (Hb) (HbA1c) is significantly associated with oxidative stress and an increased risk of cardiovascular disease (CVD). Serum total bilirubin (T-B) may have a beneficial role in preventing oxidative changes and be a negative risk factor of CVD. Limited information is available on whether serum T-B is an independent confounding factor of HbA1c. The study subjects were 633 men aged 70 ± 9 (mean ± standard deviation (SD)) years and 878 women aged 70 ± 8 years who were enrolled consecutively from among patients aged ≥40 years through a community-based annual check-up process...
January 2017: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936267/roles-of-kallikrein-2-biomarkers-free-hk2-and-pro-hk2-for-predicting-prostate-cancer-progression-free-survival
#7
Anatilde Gonzalez Guerrico, David Hillman, Jeffery Karnes, Brian Davis, Steven Gaston, George Klee
Free human kallikrein 2 (free-hK2) and hK2 pro-form (pro-hK2) have been found to be increased in tumor tissues and serum from patients with prostate cancer. We established semiautomatic assays for free-hK2 and pro-hK2 using a research version of the Beckman Coulter ACCESS2 system. Serum samples from a cohort of 189 men undergoing radical prostatectomy for known high-risk disease were assayed for Prostate-Specific Antigen (PSA), free-PSA, free-hK2, and pro-hK2. Univariate Cox regression and multivariate models were used to predict both Gleason scores and progression-free survival (PFS)...
January 2017: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936266/a-novel-method-for-the-isolation-of-extracellular-vesicles-and-rna-from-urine
#8
Anna Markowska, R Scott Pendergrast, J Stephen Pendergrast, P Shannon Pendergrast
The discovery of urinary extracellular biomarkers has been impeded by the lack of efficient methods for the isolation of extracellular vesicles (EVs: exosomes and microvesicles) and extracellular nucleic acid (RNA and DNA) from urine. Ultracentrifugation (UC), considered the gold standard for vesicle isolation from many biofluids, is efficacious but laborious, and, like most commercially available methods, is unable to isolate enough material from small volumes for protein or RNA-based biomarker discovery. We have developed a novel precipitation method for the isolation of EVs and nucleic acids from urine...
January 2017: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936265/x-rays-and-metformin-cause-increased-urinary-excretion-of-cell-free-nuclear-and-mitochondrial-dna-in-aged-rats
#9
Azhub Gaziev, Serazhutdin Abdullaev, Gulchachak Minkabirova, Kristina Kamenskikh
Activation of cell death in mammals can be assessed by an increase of an amount of cell-free DNA (cf-DNA) in urine or plasma. We investigated the excretion of cf nuclear DNA (nDNA) and cf mitochondrial DNA (mtDNA) in the urine of rats 3 and 24 months in age after X-irradiation and metformin administration. Analyses showed that prior to treatment, the amount of cf-nDNA was 40% higher and cf-mtDNA was 50% higher in the urine of aged rats compared to that of young animals. At 12 h after irradiation, the content of cf-nDNA and cf-mtDNA in the urine of young rats was increased by 200% and 460%, respectively, relative to the control, whereas in the urine of aged rats, it was 250% and 720% higher...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936264/circulating-cell-free-dna-is-a-predictor-of-short-term-neurological-outcome-in-stroke-patients-treated-with-intravenous-thrombolysis
#10
Alejandro Bustamante, Fernando Mancha, Hada C Macher, Teresa García-Berrocoso, Dolors Giralt, Marc Ribó, Juan M Guerrero, Joan Montaner
Circulating cell-free DNA (cfDNA) has been described as a prognostic marker for several diseases. Its prognostic value for short-term outcome in stroke patients treated with intravenous thrombolysis remains unexplored. cfDNA was measured on admission in 54 tissue plasminogen activator (tPA)-treated patients and 15 healthy controls using a real-time quantitative polymerase chain reaction assay. Neurological outcome was assessed at 48 h. Predictors of neurological improvement were evaluated by logistic regression analysis, and the additional predictive value of cfDNA over clinical variables was determined by integrated discrimination improvement (IDI)...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936263/novel-blood-test-to-predict-neoplastic-activity-in-healthy-patients-and-metastatic-recurrence-after-primary-tumor-resection
#11
Mohamed Abdouh, Dana Hamam, Vincenzo Arena, Manuel Arena, Hussam Alamri, Goffredo Orazio Arena
We reported that single oncosuppressor-mutated (SOM) cells turn malignant when exposed to cancer patients' sera. We tested the possibility to incorporate this discovery into a biological platform able to detect cancer in healthy individuals and to predict metastases after tumor resection. Blood was drawn prior to tumor resection and within a year after surgery. Blood samples from healthy individuals or metastatic patients were used as negative and positive controls, respectively. Patients at risk for cancer were included in the screening cohort...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936262/microvesicles-shed-from-fibroblasts-act-as-metalloproteinase-carriers-in-a-3-d-collagen-matrix
#12
Valentina Laghezza Masci, Anna Rita Taddei, Gabriella Gambellini, Franco Giorgi, Anna Maria Fausto
This study shows that fibroblasts migrating into a collagen matrix release numerous microvesicles into the surrounding medium. By spreading in regions of the matrix far distant from cells of origin, microvesicles carry metalloproteinase 9 (MMP-9) to act upon the collagen fibrils. As a result, the collagen matrix is gradually transformed from a laminar to a fibrillar type of architecture. As shown by western blots and gelatin zymography, MMP-9 is secreted as a 92 kDa precursor and activated upon release of 82 kDa product into the culture medium...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936261/the-signature-of-circulating-microparticles-in-heart-failure-patients-with-metabolic-syndrome
#13
Alexander E Berezin, Alexander Kremzer, Tatyana Berezina, Yu Martovitskaya
The role of pattern of circulating endothelial cell-derived microparticles, platelet-derived microparticles (PMPs), and monocyte-derived microparticles (MMPs) in metabolic syndrome (MetS) patients with chronic heart failure (CHF) is not still understood. The aim of the study was to investigate a pattern of circulating microparticles (MPs) in MetS patients with CHF in relation to neurohumoral and inflammatory activation. The study retrospectively involved 101 patients with MetS and 35 healthy volunteers. Biomarkers were measured at baseline of the study...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936260/addition-of-thrombin-reduces-the-recovery-of-extracellular-vesicles-from-blood-plasma
#14
Anush Arakelyan, Wendy Fitzgerald, Murad Vagida, Elena Vasilieva, Leonid Margolis, Jean-Charles Grivel
Extracellular vesicles (EVs) are widely studied as a system of intercellular communication, as markers of various diseases, as well as a vehicle for delivery of various bioactive molecules to various cells. Investigation of EVs' structure and function requires their isolation and precise quantification. However, in the current literature, there are significant discrepancies in the estimated numbers of EVs in different body fluids. In part, this discrepancy is due to the difference in EVs isolation protocols used by different investigators...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936259/the-role-of-isolation-methods-on-a-nanoscale-surface-structure-and-its-effect-on-the-size-of-exosomes
#15
JungReem Woo, Shivani Sharma, James Gimzewski
Exosomes are ∼100 nanometre diameter vesicles secreted by mammalian cells. These emerging disease biomarkers carry nucleic acids, proteins and lipids specific to the parental cells that secrete them. Exosomes are typically isolated in bulk by ultracentrifugation, filtration or immunoaffinity precipitation for downstream proteomic, genomic, or lipidomic analysis. However, the structural properties and heterogeneity of isolated exosomes at the single vesicle level are not well characterized due to their small size...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936258/detection-and-characterization-of-circulating-tumour-cells-in-multiple-myeloma
#16
Liangxuan Zhang, Sharon Beasley, Natalie L Prigozhina, Renee Higgins, Shoji Ikeda, Florence Y Lee, Dena Marrinucci, Shidong Jia
Multiple myeloma (MM) remains an incurable disease despite recent therapeutic improvements. The ability to detect and characterize MM circulating tumour cells (CTCs) in peripheral blood provides an alternative to replace or augment invasive bone marrow (BM) biopsies with a simple blood draw, providing real-time, clinically relevant information leading to improved disease management and therapy selection. Here we have developed and qualified an enrichment-free, cell-based immunofluorescence MM CTC assay that utilizes an automated digital pathology algorithm to distinguish MM CTCs from white blood cells (WBCs) on the basis of CD138 and CD45 expression levels, as well as a number of morphological parameters...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936257/protein-detection-using-the-multiplexed-proximity-extension-assay-pea-from-plasma-and-vaginal-fluid-applied-to-the-indicating-fta-elute-micro-card%C3%A2
#17
Malin Berggrund, Daniel Ekman, Inger Gustavsson, Karin Sundfeldt, Matts Olovsson, Stefan Enroth, Ulf Gyllensten
The indicating FTA elute micro card™ has been developed to collect and stabilize the nucleic acid in biological samples and is widely used in human and veterinary medicine and other disciplines. This card is not recommended for protein analyses, since surface treatment may denature proteins. We studied the ability to analyse proteins in human plasma and vaginal fluid as applied to the indicating FTA elute micro card™ using the sensitive proximity extension assay (PEA). Among 92 proteins in the Proseek Multiplex Oncology Iv2 panel, 87 were above the limit of detection (LOD) in liquid plasma and 56 among 92 above LOD in plasma applied to FTA cards...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936256/elevated-adiponectin-antibody-levels-in-sera-of-patients-with-atherosclerosis-related-coronary-artery-disease-cerebral-infarction-and-diabetes-mellitus
#18
Takaki Hiwasa, Xiao-Meng Zhang, Risa Kimura, Mikiko Ohno, Po-Min Chen, Eiichiro Nishi, Koh Ono, Takeshi Kimura, Ikuo Kamitsukasa, Takeshi Wada, Akiyo Aotsuka, Seiichiro Mine, Hirotaka Takizawa, Koichi Kashiwado, Minoru Takemoto, Kazuki Kobayashi, Harukiyo Kawamura, Ryoichi Ishibashi, Koutaro Yokote, Rika Nakamura, Go Tomiyoshi, Natsuko Shinmen, Hideyuki Kuroda
Adiponectin secreted from the adipocytes plays pleiotropic, anti-atherosclerotic roles, such as enhancement of insulin secretion and an increase in energy expenditure. The measurement of levels of circulating adiponectin is useful to evaluate the progression of atherosclerosis-related diseases, such as coronary artery disease (CAD), cerebral infarction (CI) and diabetes mellitus (DM). We examined the serum antibody levels against recombinant adiponectin protein via the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method...
January 2016: Journal of circulating biomarkers
https://www.readbyqxmd.com/read/28936255/exosome-secretion-more-than-simple-waste-disposal-implications-for-physiology-diagnostics-and-therapeutics
#19
REVIEW
Sivappriyan Nagarajah
Less than 100 nm in size and spherical in form - exosomes - vesicles expelled and taken up by cells, have ignited a new-found fascination. One which is derived from the sheer variety of exosomal content, ranging from microRNAs to transcription factors, capable of affecting a multitude of processes and pathways simultaneously within a target cell. Initially dismissed in 1983 as a waste disposal mechanism, today they form an entire field of research, being documented thus far in invertebrates, mammals, pathogens and potentially some plants...
January 2016: Journal of Circulating Biomarkers
https://www.readbyqxmd.com/read/28936254/prognostication-in-different-heart-failure-phenotypes-the-role-of-circulating-biomarkers
#20
REVIEW
Alexander E Berezin
Heart failure (HF) is multifactorial syndrome with high cardiovascular (CV) morbidity and mortality rates associated with an increasing prevalence worldwide. Measuring plasma levels of circulating biomarkers, i.e., natriuretic peptides, cardiac-specific troponins, metabolomic intermediates, Galectin-3, ST2, cardiotrophin-1, soluble endoglin and growth differentiation factor 15, may assist in the prognostication of HF development. However, the role of biomarker models in the prediction of an early stage of HF with a preserved ejection fraction (HFpEF) and HF with a reduced ejection fraction (HFrEF) is not still understood...
January 2016: Journal of Circulating Biomarkers
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