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SLAS Technology

Aaron J Walck, Kristi R Harkins
For the purposes of high-throughput immunoassay screening, PerkinElmer's AlphaLISA technology offers many benefits over traditional enzyme-linked immunosorbant assay (ELISA). However, its 680 nm excitation wavelength coincides with a wavelength of peak photosynthetic pigment absorbance, hindering the technology's utility within the plant biotechnology industry. In assays containing photosynthetic matrices, it is proposed that excitation of chlorophyll leads to the production of singlet oxygen, which initiates a pigment-associated background signal, reducing assay sensitivity...
January 10, 2019: SLAS Technology
Wenjun Guo, Sunil Kumar, Frederik Görlitz, Edwin Garcia, Yuriy Alexandrov, Ian Munro, Douglas J Kelly, Sean Warren, Peter Thorpe, Christopher Dunsby, Paul French
We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore proteins (KPs) in live budding yeast cells. The low copy number of many KPs and their small spatial extent present significant challenges for the quantification of donor fluorescence lifetime in the presence of significant cellular autofluorescence and photobleaching. Automated FLIM data acquisition was controlled by µManager and incorporated wide-field time-gated imaging with optical sectioning to reduce background fluorescence...
January 10, 2019: SLAS Technology
Heidi Fleischer, Vinh Quang Do, Kerstin Thurow
The monitoring of chemical reactions is an important task in chemical engineering, especially in quality control, pharmaceutical and biological processes, or industrial production. The development of new reactions such as catalyst-based procedures requires detailed knowledge about process steps and reaction kinetics. For qualitative and quantitative analysis of reactants and resulting products, proprietary online measurement systems are used, which were designed for special applications. A mobile online reaction monitoring system was developed for a flexible coupling to different mass selective measurement systems for structural (ESI-MS) and elemental (ICP-MS) analysis to determine chemical precursors, reaction products, and internal standard compounds and their elemental composition at any stage of the reaction...
January 7, 2019: SLAS Technology
Christopher Halbur, Niharika Choudhury, Michael Chen, Jun Hyuk Kim, Eun Ji Chung
Ovarian cancer is the fifth-most lethal cancer among women due to a lack of early detection and late-stage treatment options, and it is responsible for more than 14,000 deaths each year in the United States. Recently, there have been advances in RNA interference therapy, specifically with small interfering RNA (siRNA), to reduce tumor burden for ovarian cancer via gene down-regulation. However, delivery of siRNA poses its own challenges, as siRNA is unstable in circulation, is unable to be effectively internalized by cells, and may cause toxicity in off-target sites...
January 7, 2019: SLAS Technology
Sharon W T Chew, Yongpeng Zeng, Mingyue Cui, Hao Chang, Mengjia Zheng, Shi Wei, Wenting Zhao, Chenjie Xu
This paper introduces a facile and scalable method to generate a layer of antibacterial coating on microneedles. The antibacterial coating (i.e., zinc oxide nanobushes) is generated on the surface of gold-coated polystyrene microneedles using the hydrothermal growth method. The antimicrobial property is examined using the agar diffusion test with both gram-positive and gram-negative bacteria.
November 30, 2018: SLAS Technology
Andreas Maurer, Gregory Bowden, Jonathan Cotton, Christoph Parl, Marcel A Krueger, Bernd J Pichler
Radiosynthesis of [1-11 C]acetate is well described in literature, but all syntheses either require adaptations in complex commercial synthesizers or rely on closed-source hardware and software control. Arduino microcontrollers are ideal for the compact, flexible, and inexpensive control of low-complexity hardware, making them particularly suited for radiochemistry where operation in space-limited shielded hot cells is mandatory. We established a [1-11 C]acetate radiosynthesis module for combination with a [11 C]MeI module available in almost every lab working with 11 C...
November 30, 2018: SLAS Technology
Trevin Kurtanich, Nicole Roos, Guanmeng Wang, Jesse Yang, Alan Wang, Eun Ji Chung
Pancreatic cancer is one of the most lethal forms of cancer and has proven to be difficult to treat through conventional methods, including surgery and chemotherapy. Gene therapy serves as a potential novel treatment to interfere with genes that make this cancer so aggressive, but free nucleic acids have low cell uptake due to their negative charge and are unstable in circulation. Nanoparticles can serve as an effective carrier for a wide variety of gene therapies for pancreatic cancer as they can improve the circulation time, decrease the recognition by the immune system, and be functionalized to target specific surface proteins...
November 5, 2018: SLAS Technology
Yaw-Jen Chang, Yu-Te Lin, Chuan-Chieh Liao
This paper presents a novel design of a capillary stop valve with a chamfered side that can be used as a flow regulator to hold an injected microfluid in the valve position in a capillary force-driven microfluidic device. Biochemical analysis can be conducted if the chamfer-type valves are placed at strategic positions according to the test protocol. Hence, the stored reagent can be dragged out of the valve for further reaction when the specimen passes through. However, countercurrent phenomena were observed in the commonly used T-type capillary stop valve (without the chamfered side)...
October 25, 2018: SLAS Technology
David Yeo, Tal Murthy
No abstract text is available yet for this article.
December 2018: SLAS Technology
Kelli M Wilson, Lesley A Mathews-Griner, Tara Williamson, Rajarshi Guha, Lu Chen, Paul Shinn, Crystal McKnight, Sam Michael, Carleen Klumpp-Thomas, Zev A Binder, Marc Ferrer, Gary L Gallia, Craig J Thomas, Gregory J Riggins
Glioblastoma (GBM) is a lethal brain cancer with a median survival time of approximately 15 months following treatment. Common in vitro GBM models for drug screening are adherent and do not recapitulate the features of human GBM in vivo. Here we report the genomic characterization of nine patient-derived, spheroid GBM cell lines that recapitulate human GBM characteristics in orthotopic xenograft models. Genomic sequencing revealed that the spheroid lines contain alterations in GBM driver genes such as PTEN, CDKN2A, and NF1...
October 5, 2018: SLAS Technology
Christian Lohasz, Nassim Rousset, Kasper Renggli, Andreas Hierlemann, Olivier Frey
Microphysiological systems hold the promise to increase the predictive and translational power of in vitro substance testing owing to their faithful recapitulation of human physiology. However, the implementation of academic developments in industrial settings remains challenging. We present an injection-molded microfluidic microtissue (MT) culture chip that features two channels with 10 MT compartments each and that was designed in compliance with microtiter plate standard formats. Polystyrene as a chip material enables reliable, large-scale production and precise control over experimental conditions due to low adsorption or absorption of small, hydrophobic molecules at or into the plastic material in comparison with predecessor chips made of polydimethylsiloxane...
October 5, 2018: SLAS Technology
Richard M Eglen, Terry Reisine
Human-induced pluripotent stem cells (HiPSCs), and new technologies to culture them into functional cell types and tissues, are now aiding drug discovery. Patient-derived HiPSCs can provide disease models that are more clinically relevant and so more predictive than the currently available animal-derived or tumor cell-derived cells. These cells, consequently, exhibit disease phenotypes close to the human pathology, particularly when cultured under conditions that allow them to recapitulate the tissue architecture in three-dimensional (3D) systems...
October 4, 2018: SLAS Technology
James M Gill
No abstract text is available yet for this article.
October 2018: SLAS Technology
Alok Tayi
Over the past decade, cloud software has transformed numerous industries-from finance to logistics, marketing to manufacturing. The simplified aggregation of data, enabled by cloud computing, empowers individuals to glean insights and make data-driven decisions rapidly. In science, however, such a transformation has yet to emerge. The domain lacks centralized, machine-readable repositories of scientific data; this absence inhibits analytics and expedient decision-making. Recently, the Internet of Things (IoT) has served as a catalyst for digitizing and automating science...
October 2018: SLAS Technology
Tal Murthy, David Yeo
No abstract text is available yet for this article.
October 2018: SLAS Technology
Shiqing Zhang, Haibin Wang
Tissue engineering has progressed tremendously over recent decades through the generation of functional tissue analogs. Traditional approaches based on seeding cells into scaffold are limited in their capacity to produce tissues with precise biomimetic properties. Three-dimensional (3D) bioprinting is one kind of fabrication technology used to precisely dispense cell-laden biomaterials for the construction of functional tissues or organs. In recent years, much research progress has been made in 3D bioprinting technology and its application in generating tissue analogs, including skin, heart valves, blood vessels, bone, and cardiac tissue...
September 26, 2018: SLAS Technology
Masturah Bte Mohd Abdul Rashid, Edward Kai-Hua Chow
Artificial intelligence holds great promise in transforming how drugs are designed and patients are treated. In a study recently published in Science Translational Medicine, a unique artificial intelligence platform makes efficient use of small experimental datasets to design new drug combinations as well as identify the best drug combinations for specific patient samples. This quadratic phenotypic optimization platform (QPOP) does not rely on previous assumptions of molecular mechanisms of disease, but rather uses system-specific experimental data to determine the best drug combinations for a specific disease model or a patient sample...
September 24, 2018: SLAS Technology
Atsushi Shibai, Saburo Tsuru, Tetsuya Yomo
Ultraviolet (UV) mutagenesis is a widely used technique to increase bacterial mutation rates in laboratory experiments. UV mutagenesis requires fine regulation of UV dose, because the number of dead cells increases exponentially as the dose increases. Ignoring this hazard can cause extinction of UV-exposed populations. Therefore, an automated system that cooperatively conducts both growth measurement and UV irradiation is needed for efficient UV mutagenesis experiments. To address this task, we constructed an automated UV irradiation device for microbial cell culture...
September 10, 2018: SLAS Technology
P D Rajakumar, G-O F Gowers, L Suckling, A Foster, T Ellis, R I Kitney, D W McClymont, P S Freemont
Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences...
August 30, 2018: SLAS Technology
Adam Snider, Michael Nilsson, Mark Dupal, Masoud Toloue, Anubhav Tripathi
Next-generation sequencing technology requires amplified, short DNA fragments with known end sequences. Samples must undergo processing steps, including extraction and purification of genomic DNA (gDNA), fragmentation, end repair, adapter ligation, and amplification, to prepare a sequencing library. The process of sample preparation requires careful control of temperature and buffer conditions, as well as the stringent removal of contaminants. As a result, library preparation methods are often plagued by sample loss, long protocol times, numerous manual steps, and high cost...
August 24, 2018: SLAS Technology
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