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SLAS Technology

Brian O'Grady, Jason Wang, Shannon Faley, Daniel Balikov, Ethan Lippmann, Leon M Bellan
The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels...
May 1, 2018: SLAS Technology
Jie Wu, Meichen Pan, Huizhen Ouyang, Zhili Yang, Qiaoxin Zhang, Yingmu Cai
Arterial blood gas (ABG) analysis is important for acutely ill patients and should be performed by qualified laboratorians. The existing manual verifications are tedious, time-consuming, and prone to send wrong reports. Autoverification uses computer-based rules to verify clinical laboratory test results without manual review. To date, no data are available on the use of autoverification for ABG analysis. All autoverification rules were established according to AUTO10-A. Additionally, the rules were established using retrospective patient data, and then validated by actual clinical samples in a "live" environment before go-live...
May 1, 2018: SLAS Technology
David C Yeo, Chenjie Xu
A new study published in the journal Nature Biomedical Engineering1 documents a novel diagnostic technology that exploits topically applied nanotechnology to detect skin tissue biomarkers for diagnosis. This concept is demonstrated by noninvasively imaging connective tissue growth factor (CTGF) mRNA in abnormal scar cells, whole tissue, and animal models. In this commentary, we highlight the main findings and discuss their implications. Successful implementation in the clinic could give rise to self-applied, biopsy-free diagnostic technology and significantly reduce healthcare burden...
May 1, 2018: SLAS Technology
Pathan Fayaz Khan, S Sengottuvel, Rajesh Patel, K Gireesan, R Baskaran, Awadhesh Mani
Contact heat evoked potentials (CHEPs) are recorded from the brain by giving thermal stimulations through heating pads kept on the surface of the skin. CHEP signals have crucial diagnostic implications in human pain activation studies. This work proposes a novel design of a digital proportional integral (PI) controller based on Arduino microcontroller with a view to explore the suitability of an electric heating pad for use as a thermode in a custom-made, cost-effective CHEP stimulator. The purpose of PI controller is to set, regulate, and deliver desired temperatures on the surface of the heating pad in a user-defined pattern...
May 1, 2018: SLAS Technology
Mingyue Cui, Hao Chang, Yang Zhong, Min Wang, Tianze Wu, Xiao Hu, Zhichuan J Xu, Chenjie Xu
Pathogenic contamination and resistant bacterial infections remain critical concerns in both developed and developing countries. Rapid and sensitive detection of pathogens is still a key requirement for both environmental and clinical settings. This article introduces a simple, colorimetric, cost-effective, and high-throughput system based on a positively charged iron oxide/enzyme complex for the detection of both gram-positive and gram-negative bacteria in water between 103 and 108 cfu/mL. This study provides an effective strategy for the identification and purification of pathogen contamination in drinking water...
May 1, 2018: SLAS Technology
Brian Iglehart
Laboratory automation improves test reproducibility, which is vital to patient care in clinical laboratories. Many small and specialty laboratories are excluded from the benefits of automation due to low sample number, cost, space, and/or lack of automation expertise. The Minimum Viable Option (MVO) automation platform was developed to address these hurdles and fulfill an unmet need. Consumer 3D printing enabled rapid iterative prototyping to allow for a variety of instrumentation and assay setups and procedures...
May 1, 2018: SLAS Technology
Eric J Hill, Steve J Martin, Christopher M Weikart
The use of microplates (for bioassays, immunoassays, and general research) that are manufactured from plastic materials has proved problematic due to issues with accuracy, repeatability, and specificity of the results generated. The cause of these issues has been identified as leachables present in the plastic materials. This article presents an extractables study performed with available microplates manufactured with plastic. Common microplates from five different vendors were obtained, including plates from SiO2 Medical Products (SIO) containing a plasma treatment designed to produce an ultra-low protein-binding surface...
May 1, 2018: SLAS Technology
Antti-Juhana Mäki, Jarmo Verho, Joose Kreutzer, Tomi Ryynänen, Dhanesh Rajan, Mari Pekkanen-Mattila, Antti Ahola, Jari Hyttinen, Katriina Aalto-Setälä, Jukka Lekkala, Pasi Kallio
A physiologically relevant environment is essential for successful long-term cell culturing in vitro. Precise control of temperature, one of the most crucial environmental parameters in cell cultures, increases the fidelity and repeatability of the experiments. Unfortunately, direct temperature measurement can interfere with the cultures or prevent imaging of the cells. Furthermore, the assessment of dynamic temperature variations in the cell culture area is challenging with the methods traditionally used for measuring temperature in cell culture systems...
April 1, 2018: SLAS Technology
Mridu Sinha, Hannah Mack, Todd P Coleman, Stephanie I Fraley
DNA melting analysis provides a rapid method for genotyping a target amplicon directly after PCR amplification. To transform melt genotyping into a broad-based profiling approach for heterogeneous samples, we previously proposed the integration of universal PCR and melt analysis with digital PCR. Here, we advanced this concept by developing a high-resolution digital melt platform with precise thermal control to accomplish reliable, high-throughput heat ramping of microfluidic chip digital PCR reactions. Using synthetic DNA oligos with defined melting temperatures, we characterized sources of melting variability and minimized run-to-run variations...
April 1, 2018: SLAS Technology
Justin Shumate, Pierre Baillargeon, Timothy P Spicer, Louis Scampavia
Critical to maintaining quality control in high-throughput screening is the need for constant monitoring of liquid-dispensing fidelity. Traditional methods involve operator intervention with gravimetric analysis to monitor the gross accuracy of full plate dispenses, visual verification of contents, or dedicated weigh stations on screening platforms that introduce potential bottlenecks and increase the plate-processing cycle time. We present a unique solution using open-source hardware, software, and 3D printing to automate dispenser accuracy determination by providing real-time dispense weight measurements via a network-connected precision balance...
April 1, 2018: SLAS Technology
Corey E Reed, Jennifer Fournier, Nikolaos Vamvoukas, Stephan M Koza
N-Glycan analysis is routinely performed for biotherapeutic protein characterization. A recently introduced N-glycan analysis kit using RapiFluor-MS (RFMS) labeling provides time savings over reductive amination labeling methods while also providing enhanced fluorescence (FLR) and mass spectrometry (MS) responses. This article demonstrates the semiautomation of this kit using an Andrew Alliance pipetting robot that promises further gains in productivity. This robotic platform uses standard manual pipettors and an optically guided arm to facilitate the automation of manual procedures...
March 1, 2018: SLAS Technology
Jean Courtemanche, Samson King, David Bouck
The application of 3D printing and microcontrollers allows users to rapidly engineer novel hardware solutions useful in a laboratory environment. 3D printing is transformative as it enables the rapid fabrication of adapters, housings, jigs, and small structural elements. Microcontrollers allow for the creation of simple, inexpensive machines that receive input from one or more sensors to trigger a mechanical or electrical output. Bringing these technologies together, we have developed custom solutions that improve capabilities and reduce costs, errors, and human intervention...
March 1, 2018: SLAS Technology
Anand D Jeyasekharan
No abstract text is available yet for this article.
June 2018: SLAS Technology
Tal Murthy, David Yeo
No abstract text is available yet for this article.
June 2018: SLAS Technology
S Sengottuvel, Pathan Fayaz Khan, N Mariyappa, Rajesh Patel, S Saipriya, K Gireesan
Cutaneous measurements of electrogastrogram (EGG) signals are heavily contaminated by artifacts due to cardiac activity, breathing, motion artifacts, and electrode drifts whose effective elimination remains an open problem. A common methodology is proposed by combining independent component analysis (ICA) and ensemble empirical mode decomposition (EEMD) to denoise gastric slow-wave signals in multichannel EGG data. Sixteen electrodes are fixed over the upper abdomen to measure the EGG signals under three gastric conditions, namely, preprandial, postprandial immediately, and postprandial 2 h after food for three healthy subjects and a subject with a gastric disorder...
June 2018: SLAS Technology
David Yeo, Tal Murthy
No abstract text is available yet for this article.
April 2018: SLAS Technology
Junwei Lv, Alok Sharma, Ting Zhang, Yuchen Wu, Xianting Ding
Natural triterpenes represent a group of pharmacologically active and structurally diverse organic compounds. The focus on these phytochemicals has been enormous in the past few years, worldwide. Asiatic acid (AA), a naturally occurring pentacyclic triterpenoid, is found mainly in the traditional medicinal herb Centella asiatica. Triterpenoid saponins, which are the primary constituents of C. asiatica, are commonly believed to be responsible for their extensive therapeutic actions. Published research work has described the molecular mechanisms underlying the various biological activities of AA and its derivatives, which vary for each chronic disease...
April 2018: SLAS Technology
Benjamin Gille, Lieselot Dedeene, Erik Stoops, Leentje Demeyer, Cindy Francois, Stefanie Lefever, Maxim De Schaepdryver, Britta Brix, Rik Vandenberghe, Jos Tournoy, Hugo Vanderstichele, Koen Poesen
The lack of (inter-)laboratory standardization has hampered the application of universal cutoff values for Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers and their transfer to general clinical practice. The automation of the AD biomarker immunoassays is suggested to generate more robust results than using manual testing. Open-access platforms will facilitate the integration of automation for novel biomarkers, allowing the introduction of the protein profiling concept. A feasibility study was performed on an automated open-access platform of the commercial immunoassays for the 42-amino-acid isoform of amyloid-β (Aβ1-42 ), Aβ1-40 , and total tau in CSF...
April 2018: SLAS Technology
Yì Xiáng J Wáng, Min Deng, Jiang Lin, Anthony W L Kwok, Eric K W Liu, Weitian Chen
To understand women's and men's physiological ranges of liver T1rho relaxation time measured with a single breathhold black blood sequence, this healthy volunteer study was conducted in 62 women (mean age, 38.9 y; range, 18-75 y) and 34 men (mean age, 44.7 y; range, 24-80 y). Approval from the institutional ethics committee was obtained. Magnetic resonance imaging was performed with a 3.0T scanner with six spin-lock times of 0, 10, 20, 25, 35, and 50 ms and a single breathhold of 12 s per slice acquisition...
April 2018: SLAS Technology
Yaw-Jen Chang, Yi-Hua Fan, Shia-Chung Chen, Kuan-Hua Lee, Liao-Yong Lou
A blood-typing assay is a critical test to ensure the serological compatibility of a donor and an intended recipient prior to a blood transfusion. This article presents a lab-on-disc blood-typing system to conduct a total of eight assays for a patient, including forward-typing tests, reverse-typing tests, and irregular-antibody tests. These assays are carried out in a microfluidic disc simultaneously. A blood-typing apparatus was designed to automatically manipulate the disc. The blood type can be determined by integrating the results of red blood cell (RBC) agglutination in the microchannels...
April 2018: SLAS Technology
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