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SLAS Technology

Duncan M Chadly, Andrew M Oleksijew, Kyle S Coots, Jose J Fernandez, Shun Kobayashi, John A Kessler, Akihiro J Matsuoka
Human pluripotent stem cells (hPSCs) are promising therapeutic tools for regenerative therapies and disease modeling. Differentiation of cultured hPSCs is influenced by both exogenous factors added to the cultures and endogenously secreted molecules. Optimization of protocols for the differentiation of hPSCs into different cell types is difficult because of the many variables that can influence cell fate. We present microfluidic devices designed to perform three- and four-factor, two-level full factorial experiments in parallel for investigating and directly optimizing hPSC differentiation...
July 1, 2018: SLAS Technology
Pathan Fayaz Khan, S Sengottuvel, Rajesh Patel, Awadhesh Mani, K Gireesan
Liquid helium (LHe) is used as a cryogen in a variety of applications involving superconductivity and is routinely monitored for conducting low-temperature experiments. Thermoacoustic oscillations, which are inevitably present inside closed LHe containers, are utilized for level detection by sensing the vibrations at the warm end of a thin capillary tube inserted into the Dewar. The position of the capillary tube at which a sudden change occurs in these oscillations is manually sensed to identify the liquid level...
July 1, 2018: SLAS Technology
Andreas Osterman, Patricia M Späth, Gundula Jäger, Stephan Böhm, Peter Osterman
The determination of pathogen-specific antibody indices (AIs) of CSF and serum is an essential cornerstone in assessing neurological diseases and demands reliable high precision. Various companies provide ELISA kits for the detection of respective antibody concentrations and base AI calculation on a single CSF/serum pair of optical densities (ODs), combined with selection rules. The remainder of OD measurements is not used. There is no averaging of measurement errors and result stabilization. OD data from Siemens Enzygnost ELISA measurements of 2012-2016 proficiency survey samples for measles/rubella/varicella zoster/herpes simplex virus (MRZH) reaction (INSTAND e...
June 1, 2018: SLAS Technology
Yong-Jun Ko, Joon-Ho Maeng, Seung Yong Hwang, Yoomin Ahn
Infertile couples needing assisted reproduction are increasing, so a fundamental understanding of motile sperm migration is required. This paper presents an advanced microfluidic device for sperm motion analysis utilizing chemotaxis and thermotaxis simultaneously for the first time. The proposed device is a transparent polydimethylsiloxane- and glass-based microfluidic chip system providing a low-cost, useful, and disposable platform for sperm analysis. The concentration gradient of the chemoattractant (acetylcholine) and the temperature difference are formed along the microchannel...
June 1, 2018: SLAS Technology
John Lalith Charles Richard, Pieter Johan Adam Eichhorn
Prior to the sequencing of the human genome, it was presumed that most of the DNA coded for proteins. However, with the advent of next-generation sequencing, it has now been recognized that most complex eukaryotic genomes are in fact transcribed into noncoding RNAs (ncRNAs), including a family of transcripts referred to as long noncoding RNAs (lncRNAs). LncRNAs have been implicated in many biological processes ranging from housekeeping functions such as transcription to more specialized functions such as dosage compensation or genomic imprinting, among others...
June 1, 2018: SLAS Technology
Francis R Cui, Blanche C Ip, Jeffrey R Morgan, Anubhav Tripathi
Technological advances in solid organ tissue engineering that rely on the assembly of small tissue-building parts require a novel transport method suited for soft, deformable, living objects of submillimeter- to centimeter-length scale. We describe a technology that utilizes membrane flow through a gripper to generate optimized pressure differentials across the top and bottom surfaces of microtissue so that the part may be gripped and lifted. The flow and geometry parameters are developed for automation by analyzing the fluid mechanics framework by which a gripper can lift tissue parts off solid and porous surfaces...
June 1, 2018: SLAS Technology
Sandra Laternser, Hansjoerg Keller, Olivier Leupin, Martin Rausch, Ursula Graf-Hausner, Markus Rimann
Two-dimensional (2D) cell cultures do not reflect the in vivo situation, and thus it is important to develop predictive three-dimensional (3D) in vitro models with enhanced reliability and robustness for drug screening applications. Treatments against muscle-related diseases are becoming more prominent due to the growth of the aging population worldwide. In this study, we describe a novel drug screening platform with automated production of 3D musculoskeletal-tendon-like tissues. With 3D bioprinting, alternating layers of photo-polymerized gelatin-methacryloyl-based bioink and cell suspension tissue models were produced in a dumbbell shape onto novel postholder cell culture inserts in 24-well plates...
June 1, 2018: SLAS Technology
Yangjie Wei, Nicholas R Larson, Siva K Angalakurthi, C Russell Middaugh
The goal of protein formulation development is to identify optimal conditions for long-term storage. Certain commercial conditions (e.g., high protein concentration or turbid adjuvanted samples) impart additional challenges to biophysical characterization. Formulation screening studies for such conditions are usually performed using a simplified format in which the target protein is studied at a low concentration in a clear solution. The failure of study conditions to model the actual formulation environment may cause a loss of ability to identify the optimal condition for target proteins in their final commercial formulations...
June 1, 2018: SLAS Technology
Yinshen Wee, Amanda N Moore, Shihai Jia, Jing Zhou, John S Colombo, Rena N D'Souza
Hydrogels are homogenous materials that are limited in their ability to form oriented multilayered architecture in three-dimensional (3D) tissue constructs. Current techniques have led to advancements in this area. Such techniques often require extra devices and/or involve complex processes that are inaccessible to many laboratories. Here is described a one-step methodology that permits reliable alignment of cells into multiple layers using a self-assembling multidomain peptide (MDP) hydrogels. We characterized the structural features, viability, and molecular properties of dental pulp cells fabricated with MDP and demonstrated that manipulation of the layering of cells in the scaffolds was achieved by decreasing the weight by volume percentage (w/v%) of MDP contained within the scaffold...
May 1, 2018: SLAS Technology
T Volden, J Goldowsky, N Schmid, V Revol
Precise metering in liquid dispensing applications often requires application-specific solutions due to incompatibilities of the sensor and actuator components with the dispensed liquids. Some reoccurring challenges are aggressive liquids that would damage the sensors or tubing, the need for sterile liquids while the pumps or sensors cannot be sterilized, or media that can clog the sensor channels. Two different dispensing systems are here presented where the dispensing flow rate or volume is indirectly measured through a coupled pressure change or airflow, thus avoiding contact between the sensor and liquid...
May 1, 2018: SLAS Technology
Brian O'Grady, Jason Wang, Shannon Faley, Daniel Balikov, Ethan Lippmann, Leon M Bellan
The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels...
May 1, 2018: SLAS Technology
Jie Wu, Meichen Pan, Huizhen Ouyang, Zhili Yang, Qiaoxin Zhang, Yingmu Cai
Arterial blood gas (ABG) analysis is important for acutely ill patients and should be performed by qualified laboratorians. The existing manual verifications are tedious, time-consuming, and prone to send wrong reports. Autoverification uses computer-based rules to verify clinical laboratory test results without manual review. To date, no data are available on the use of autoverification for ABG analysis. All autoverification rules were established according to AUTO10-A. Additionally, the rules were established using retrospective patient data, and then validated by actual clinical samples in a "live" environment before go-live...
May 1, 2018: SLAS Technology
David C Yeo, Chenjie Xu
A new study published in the journal Nature Biomedical Engineering1 documents a novel diagnostic technology that exploits topically applied nanotechnology to detect skin tissue biomarkers for diagnosis. This concept is demonstrated by noninvasively imaging connective tissue growth factor (CTGF) mRNA in abnormal scar cells, whole tissue, and animal models. In this commentary, we highlight the main findings and discuss their implications. Successful implementation in the clinic could give rise to self-applied, biopsy-free diagnostic technology and significantly reduce healthcare burden...
May 1, 2018: SLAS Technology
Pathan Fayaz Khan, S Sengottuvel, Rajesh Patel, K Gireesan, R Baskaran, Awadhesh Mani
Contact heat evoked potentials (CHEPs) are recorded from the brain by giving thermal stimulations through heating pads kept on the surface of the skin. CHEP signals have crucial diagnostic implications in human pain activation studies. This work proposes a novel design of a digital proportional integral (PI) controller based on Arduino microcontroller with a view to explore the suitability of an electric heating pad for use as a thermode in a custom-made, cost-effective CHEP stimulator. The purpose of PI controller is to set, regulate, and deliver desired temperatures on the surface of the heating pad in a user-defined pattern...
May 1, 2018: SLAS Technology
Mingyue Cui, Hao Chang, Yang Zhong, Min Wang, Tianze Wu, Xiao Hu, Zhichuan J Xu, Chenjie Xu
Pathogenic contamination and resistant bacterial infections remain critical concerns in both developed and developing countries. Rapid and sensitive detection of pathogens is still a key requirement for both environmental and clinical settings. This article introduces a simple, colorimetric, cost-effective, and high-throughput system based on a positively charged iron oxide/enzyme complex for the detection of both gram-positive and gram-negative bacteria in water between 103 and 108 cfu/mL. This study provides an effective strategy for the identification and purification of pathogen contamination in drinking water...
May 1, 2018: SLAS Technology
Brian Iglehart
Laboratory automation improves test reproducibility, which is vital to patient care in clinical laboratories. Many small and specialty laboratories are excluded from the benefits of automation due to low sample number, cost, space, and/or lack of automation expertise. The Minimum Viable Option (MVO) automation platform was developed to address these hurdles and fulfill an unmet need. Consumer 3D printing enabled rapid iterative prototyping to allow for a variety of instrumentation and assay setups and procedures...
May 1, 2018: SLAS Technology
Eric J Hill, Steve J Martin, Christopher M Weikart
The use of microplates (for bioassays, immunoassays, and general research) that are manufactured from plastic materials has proved problematic due to issues with accuracy, repeatability, and specificity of the results generated. The cause of these issues has been identified as leachables present in the plastic materials. This article presents an extractables study performed with available microplates manufactured with plastic. Common microplates from five different vendors were obtained, including plates from SiO2 Medical Products (SIO) containing a plasma treatment designed to produce an ultra-low protein-binding surface...
May 1, 2018: SLAS Technology
Anand D Jeyasekharan
No abstract text is available yet for this article.
June 2018: SLAS Technology
Tal Murthy, David Yeo
No abstract text is available yet for this article.
June 2018: SLAS Technology
S Sengottuvel, Pathan Fayaz Khan, N Mariyappa, Rajesh Patel, S Saipriya, K Gireesan
Cutaneous measurements of electrogastrogram (EGG) signals are heavily contaminated by artifacts due to cardiac activity, breathing, motion artifacts, and electrode drifts whose effective elimination remains an open problem. A common methodology is proposed by combining independent component analysis (ICA) and ensemble empirical mode decomposition (EEMD) to denoise gastric slow-wave signals in multichannel EGG data. Sixteen electrodes are fixed over the upper abdomen to measure the EGG signals under three gastric conditions, namely, preprandial, postprandial immediately, and postprandial 2 h after food for three healthy subjects and a subject with a gastric disorder...
June 2018: SLAS Technology
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