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SLAS Technology

Atsushi Shibai, Saburo Tsuru, Tetsuya Yomo
Ultraviolet (UV) mutagenesis is a widely used technique to increase bacterial mutation rates in laboratory experiments. UV mutagenesis requires fine regulation of UV dose, because the number of dead cells increases exponentially as the dose increases. Ignoring this hazard can cause extinction of UV-exposed populations. Therefore, an automated system that cooperatively conducts both growth measurement and UV irradiation is needed for efficient UV mutagenesis experiments. To address this task, we constructed an automated UV irradiation device for microbial cell culture...
September 10, 2018: SLAS Technology
P D Rajakumar, G-O F Gowers, L Suckling, A Foster, T Ellis, R I Kitney, D W McClymont, P S Freemont
Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences...
August 30, 2018: SLAS Technology
Adam Snider, Michael Nilsson, Mark Dupal, Masoud Toloue, Anubhav Tripathi
Next-generation sequencing technology requires amplified, short DNA fragments with known end sequences. Samples must undergo processing steps, including extraction and purification of genomic DNA (gDNA), fragmentation, end repair, adapter ligation, and amplification, to prepare a sequencing library. The process of sample preparation requires careful control of temperature and buffer conditions, as well as the stringent removal of contaminants. As a result, library preparation methods are often plagued by sample loss, long protocol times, numerous manual steps, and high cost...
August 24, 2018: SLAS Technology
Kaylee R Mastrianni, William E Kemnitzer, Kevin W P Miller
Catecholamines are integral neurotransmitters in the central and peripheral nervous system. Clinically, catecholamine levels are determined to help diagnose disease and measure corresponding therapeutic effectiveness. However, manual extraction of catecholamines and their metabolites may be labor-intensive and user-variable and require a variety of peripheral laboratory devices, especially at low sample concentrations. Here, we propose a novel solid-phase extraction (SPE) method using patented dispersive pipette extraction (DPX) tip technology...
August 15, 2018: SLAS Technology
Jong-Won Lee, Kyoung-Bong Ha, Youn-Kyu Kim, Joo-Hee Lee, In-Ho Choi, Seul-Hyun Park
Life science research has been actively carried out in space for a long time using bioreactor equipment, in anticipation of manned space exploration and space tourism. Such studies have reported that the microgravity environment has a negative effect on the human body, including the musculoskeletal system, nervous system, and endocrine system. Bone loss and muscular atrophy are issues that need to be resolved before long-term exposure of the human body to a space environment. To address this problem, Y. K. Kim et al...
August 10, 2018: SLAS Technology
Martin Winter, Robert Ries, Carola Kleiner, Daniel Bischoff, Andreas H Luippold, Tom Bretschneider, Frank H B├╝ttner
Label-free, mass spectrometric (MS) deciphering of enzymatic reactions by direct analysis of substrate-to-product conversion provides the next step toward more physiological relevant assays within drug discovery campaigns. Reduced risk of suffering from compound interference combined with diminished necessity for tailored signal mediators emphasizes the valuable role of label-free readouts. However, MS-based detection has not hitherto met high-throughput screening (HTS) requirements because of the lack of HTS-compatible sample introduction...
August 3, 2018: SLAS Technology
Xue Li, Bo Chen, Shaoya Zhang, Xiuyuan Li, Junxia Chang, Yanyan Tang, Yong Wu, Xinxin Lu
Community-acquired pneumonia (CAP) is a common infectious disease linked to high rates of morbidity and mortality. Fast and accurate identification of the pathogens responsible for CAP will aid in diagnosis. We established a capillary electrophoresis-based multiplex PCR (CEMP) panel to enable the detection of viral and bacterial pathogens associated with CAP. The assay simultaneously detects and identifies the 13 common unculturable CAP viral and bacterial pathogens within 4 h. We evaluated the performance of a commercially available panel with 314 samples collected from CAP patients...
July 26, 2018: SLAS Technology
Ben Miles, Peter L Lee
A robotic cloud laboratory driven by a state-of-the-art unified laboratory operating system integrates automated hardware, humans, and sensors. This lab of the future system enables researchers to transparently and collaboratively create, optimize, and organize biological experiments to achieve more reproducible results, perform around-the-clock experimentation, and more efficiently navigate the vast parameter space of biology.
July 25, 2018: SLAS Technology
Jonas Austerjost, Marc Porr, Noah Riedel, Dominik Geier, Thomas Becker, Thomas Scheper, Daniel Marquard, Patrick Lindner, Sascha Beutel
The introduction of smart virtual assistants (VAs) and corresponding smart devices brought a new degree of freedom to our everyday lives. Voice-controlled and Internet-connected devices allow intuitive device controlling and monitoring from all around the globe and define a new era of human-machine interaction. Although VAs are especially successful in home automation, they also show great potential as artificial intelligence-driven laboratory assistants. Possible applications include stepwise reading of standard operating procedures (SOPs) and recipes, recitation of chemical substance or reaction parameters to a control, and readout of laboratory devices and sensors...
July 1, 2018: SLAS Technology
William Neil, Greg Zipp, Gregory Nemeth, Mark F Russo, David S Nirschl
We describe a custom Internet of Things (IoT) device used for tracking barcoded containers end to end in a high-throughput analysis and purification laboratory. Our IoT device fills an important gap that previously prevented us from fully tracking barcoded sample containers through manual steps in a multistep workflow, such as when samples are "parked" for temporary storage, or when using instrumentation not otherwise equipped with barcode scanners, a common occurrence found with specific centrifugal evaporation instruments...
July 1, 2018: SLAS Technology
Duncan M Chadly, Andrew M Oleksijew, Kyle S Coots, Jose J Fernandez, Shun Kobayashi, John A Kessler, Akihiro J Matsuoka
Human pluripotent stem cells (hPSCs) are promising therapeutic tools for regenerative therapies and disease modeling. Differentiation of cultured hPSCs is influenced by both exogenous factors added to the cultures and endogenously secreted molecules. Optimization of protocols for the differentiation of hPSCs into different cell types is difficult because of the many variables that can influence cell fate. We present microfluidic devices designed to perform three- and four-factor, two-level full factorial experiments in parallel for investigating and directly optimizing hPSC differentiation...
July 1, 2018: SLAS Technology
Pathan Fayaz Khan, S Sengottuvel, Rajesh Patel, Awadhesh Mani, K Gireesan
Liquid helium (LHe) is used as a cryogen in a variety of applications involving superconductivity and is routinely monitored for conducting low-temperature experiments. Thermoacoustic oscillations, which are inevitably present inside closed LHe containers, are utilized for level detection by sensing the vibrations at the warm end of a thin capillary tube inserted into the Dewar. The position of the capillary tube at which a sudden change occurs in these oscillations is manually sensed to identify the liquid level...
July 1, 2018: SLAS Technology
James M Gill
No abstract text is available yet for this article.
October 2018: SLAS Technology
Alok Tayi
Over the past decade, cloud software has transformed numerous industries-from finance to logistics, marketing to manufacturing. The simplified aggregation of data, enabled by cloud computing, empowers individuals to glean insights and make data-driven decisions rapidly. In science, however, such a transformation has yet to emerge. The domain lacks centralized, machine-readable repositories of scientific data; this absence inhibits analytics and expedient decision-making. Recently, the Internet of Things (IoT) has served as a catalyst for digitizing and automating science...
October 2018: SLAS Technology
Tal Murthy, David Yeo
No abstract text is available yet for this article.
October 2018: SLAS Technology
David Yeo, Tal Murthy
No abstract text is available yet for this article.
August 2018: SLAS Technology
C Surrette, B Scherer, A Corwin, G Grossmann, A M Kaushik, K Hsieh, P Zhang, J C Liao, P K Wong, T H Wang, C M Puleo
Recently advances in miniaturization and automation have been utilized to rapidly decrease the time to result for microbiology testing in the clinic. These advances have been made due to the limitations of conventional culture-based microbiology methods, including agar plate and microbroth dilution, which have long turnaround times and require physicians to treat patients empirically with antibiotics before test results are available. Currently, there exist similar limitations in pharmaceutical sterility and bioburden testing, where the long turnaround times associated with standard microbiology testing drive costly inefficiencies in workflows...
August 2018: SLAS Technology
C M Puleo, B Davis, R Smith
No abstract text is available yet for this article.
August 2018: SLAS Technology
David C Yeo, Chenjie Xu
A new study published in the journal Nature Biomedical Engineering1 documents a novel diagnostic technology that exploits topically applied nanotechnology to detect skin tissue biomarkers for diagnosis. This concept is demonstrated by noninvasively imaging connective tissue growth factor (CTGF) mRNA in abnormal scar cells, whole tissue, and animal models. In this commentary, we highlight the main findings and discuss their implications. Successful implementation in the clinic could give rise to self-applied, biopsy-free diagnostic technology and significantly reduce healthcare burden...
August 2018: SLAS Technology
Brian M Davis, Evelina R Loghin, Kenneth R Conway, Xiaohua Zhang
Pluripotent stem cell suspension aggregates have proven to be an efficient and phenotypically stable means for expansion and directed differentiation. Bioreactor systems with automation of perfusion, fluidization, and gas exchange are essential for scaling up pluripotent stem cell cultures. Since stem cell pluripotency and differentiation are affected by both chemical and physical signals, we investigated a low-shear method for the expansion of cells in a rocking-motion bioreactor. The rocking motion drives continual mixing and aeration, and the single-use disposable bioreactors avoid issues around contamination during seeding, medium exchange, passage, and cell harvest...
August 2018: SLAS Technology
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