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Italian Journal of Food Safety

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https://www.readbyqxmd.com/read/28713795/a-new-approach-to-predict-the-fish-fillet-shelf-life-in-presence-of-natural-preservative-agents
#1
Alessandro Giuffrida, Filippo Giarratana, Davide Valenti, Daniele Muscolino, Roberta Parisi, Alessio Parco, Stefania Marotta, Graziella Ziino, Antonio Panebianco
Three data sets concerning the behaviour of spoilage flora of fillets treated with natural preservative substances (NPS) were used to construct a new kind of mathematical predictive model. This model, unlike other ones, allows expressing the antibacterial effect of the NPS separately from the prediction of the growth rate. This approach, based on the introduction of a parameter into the predictive primary model, produced a good fitting of observed data and allowed characterising quantitatively the increase of shelf-life of fillets...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713794/correction-occurrence-of-harmful-algal-species-and-shellfish-toxicity-in-sardinia-italy
#2
Anna Maria Bazzoni, Alessandro Graziano Mudadu, Giuseppa Lorenzoni, Igor Arras, Antonella Lugliè, Barbara Vivaldi, Valentina Ciccotelli, Giovanna Sanna, Giuseppe Tedde, Salvatore Ledda, Enrico Alesso, Edoardo Marongiu, Sebastiano Virgilio
[This corrects the article on p. 6095 in vol. 5, PMID: 28058244.].
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713793/phenotypic-antimicrobial-resistance-profile-of-isolates-causing-clinical-mastitis-in-dairy-animals
#3
Carlotta Ceniti, Domenico Britti, Adriano Michele Luigi Santoro, Rosanna Musarella, Lucia Ciambrone, Francesco Casalinuovo, Nicola Costanzo
Mastitis is the most frequent and costly disease of lactating animals and is associated with a significant reduction in milk yield, increased cost and culling. Early and specific antibiotic based treatment reduces the severity of the disease. Over the years the extensive use of antimicrobials has led to increase antimicrobial resistance. The present study was designed to investigate the prevalence of microorganisms responsible for mastitis and their antimicrobial resistance pattern. A total of 282 milk samples were collected from different animal species (sheep, cows and goats) with clinical mastitis...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713792/shiga-toxin-producing-escherichia-coii-in-slaughtered-pigs-and-pork-products
#4
Lia Bardasi, Roberta Taddei, Ilaria Fiocchi, Maria Francesca Pelliconi, Mattia Ramini, Elena Toschi, Giuseppe Merialdi
During the years 2015-2016, 83 faecal samples were collected at slaughter from pigs reared in farms located in Central-Northern Italy. During the years 2014-2016 a total of 562 pork products [465 not-readyto-eat (NRTE) and 97 ready-to-eat (RTE) products] were collected from retail outlets, large retailers and processing plants. The samples were analysed according to ISO TS 13136:2012. Out of 83 swine faecal samples, 77 (92.8%) resulted stx-positive by real time polymerase chain reaction (PCR), 5 stx2+ and 1 stx1+ Shiga toxin-producing Escherichia coli (STEC) strains were isolated...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713791/detection-of-sulfites-in-fresh-meat-preparation-commercialised-at-retail-in-lazio-region
#5
Giuseppe Carrabs, Giorgio Smaldone, Leonardo Carosielli, Mariagrazia Girasole, Marco Iammarino, Eugenio Chiaravalle
In Europe sulfur dioxide (SO2) and sulfites in foods and beverages at concentrations of more than 10 mg kg(-1) or 10 mg L(-1) expressed as SO2 equivalents are subject to mandatory labelling. In fresh meats the addition of sulfites is not admitted because of their unlawful use to give the product a more attractive appearance. Aim of the study was to detect sulfites in meat product sampled in 19 commercial shops of Lazio Region. In n=12 samples, sulfites were present at different concentrations and no indication for them was reported on the label...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713790/preliminary-study-on-physicochemical-and-biochemical-stress-markers-at-poultry-slaughterhouse
#6
Serena Santonicola, Maria Francesca Peruzy, Mariagrazia Girasole, Nicoletta Murru, Maria Luisa Cortesi, Raffaelina Mercogliano
Pre-slaughter stress can result in variations in the glycogen storage and metabolic changes of muscle, responsible for quality poultry meat. Aim of this study was to investigate, as pre-slaughter stress markers and quality meat, physicochemical (pH), biochemical (muscle glycogen content), and chemical (super oxides free radicals) parameters. The carcass quality, as incidence of individual carcass defects, was also evaluated. Twenty broilers were processed with two different electrical stunning: high (250 Hz; 640 mA; 60V) (Lot C or control) and low (150 Hz; 360 mA; 60 V) (Lot A) frequency and intensity, using sinusoidal alternating current...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713789/screening-of-oxalate-degrading-lactic-acid-bacteria-of-food-origin
#7
Nicoletta Murru, Giuseppe Blaiotta, Maria Francesca Peruzy, Serena Santonicola, Raffaelina Mercogliano, Maria Aponte
A screening for oxalate degrading abilities was initially carried on within Lactic Acid Bacteria cultures of different food origin. Seventy-nine strains were drop-inoculated onto MRS agar plates containing calcium oxalate. By comparing colonies diameters, 31 strains were used to inoculate, in parallel, MRS and MRS modified by sodium oxalate addition. Differences in the strains' growth were assessed by colony forming unit counts. For two strains, the growth in oxalate enriched medium was significantly higher; while, for eleven strains an opposite behaviour was recorded...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713788/microorganisms-of-the-intestinal-microbiota-of-oncorhynchus-mykiss-produce-antagonistic-substances-against-bacteria-contaminating-food-and-causing-disease-in-humans
#8
Olga Lobos, Andrea Barrera, Carlos Padilla
The objective was to analyse the antibacterial ability of members of the intestinal microbiota of Oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. Bacterial colonies from an intestinal portion of the 20 specimens of O. mykiss were obtained in different culture media. Several of the colonies showed antibacterial action on different target strains. The bacterial species with the highest antagonistic capacity were Hafnia alvei and Lactococcus lactis and the more susceptible target strains were Aeromonas hydrophila, Listeria monocytogenes and Escherichia coli...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713787/consumers-preferences-for-a-local-food-product-the-case-of-a-new-carnaroli-rice-product-in-lombardy
#9
Giovanni Ferrazzi, Vera Ventura, Sabrina Ratti, Claudia Balzaretti
Italy, with a cultivated area of 218,000 ha, is a European leader of rice production. In particular Lombardy region accounts for 40% of total rice cultivation and the case study in object accounts for 3.2% of Lombardy total rice area (2773 ha). Starting from 2012, through a regional project titled Buono, Sano e Vicino (good, healthy and close), Riso e Rane rural district supported local rice farmers in developing innovation in rice production and promoting an alternative supply chain to increase farmers bargaining power and promote new market strategies...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713786/development-of-a-method-to-extract-and-amplify-the-complete-mitogenome-of-some-sparidae-species
#10
Celestina Mascolo, Marina Ceruso, Paolo Sordino, Giuseppe Palma, Aniello Anastasio, Tiziana Pepe
Previous studies showed that fish mitochondrial DNA (mtDNA) is set up by a closed circular molecule of 16-17 kilobases (kb), comprising 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), 13 protein-coding genes and 2 non-coding regions. The analysis of single mtDNA genes, such as Cytb, COI, 16S and 12S, or short segment of them, has been widely used against species substitution in both fresh and processed fish products. The analysis of the complete mitochondrial genome of fishery products allows to better study and characterise fish species...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713785/inactivation-of-heat-adapted-and-chlorine-adapted-listeria-monocytogenes-atcc-7644-on-tomatoes-using-sodium-dodecyl-sulphate-levulinic-acid-and-sodium-hypochlorite-solution
#11
Oluwatosin Ademola Ijabadeniyi, Elizabeth Mnyandu
The effectiveness of sodium dodecyl sulphate (SDS), sodium hypochlorite solution and levulinic acid in reducing the survival of heat adapted and chlorine adapted Listeria monocytogenes ATCC 7644 was evaluated. The results against heat adapted L. monocytognes revealed that sodium hypochlorite solution was the least effective, achieving log reduction of 2.75, 2.94 and 3.97 log colony forming unit (CFU)/mL for 1, 3 and 5 minutes, respectively. SDS was able to achieve 8 log reduction for both heat adapted and chlorine adapted bacteria...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462207/toxoplasma-gondii-and-pre-treatment-protocols-for-polymerase-chain-reaction-analysis-of-milk-samples-a-field-trial-in-sheep-from-southern-italy
#12
Alice Vismarra, Elena Barilli, Maura Miceli, Carlo Mangia, Cristina Bacci, Franco Brindani, Laura Kramer
Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii. Ingestion of raw milk has been suggested as a risk for transmission to humans. Here the authors evaluated pre-treatment protocols for DNA extraction on T. gondii tachyzoite-spiked sheep milk with the aim of identifying the method that resulted in the most rapid and reliable polymerase chain reaction (PCR) positivity. This protocol was then used to analyse milk samples from sheep of three different farms in Southern Italy, including real time PCR for DNA quantification and PCR-restriction fragment length polymorphism for genotyping...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462206/formulation-and-shelf-life-of-fish-burgers-served-to-preschool-children
#13
Giorgio Smaldone, Raffaele Marrone, Tiziana Zottola, Lucia Vollano, Giulio Grossi, Maria Luisa Cortesi
Consumer is very careful about healthiness; in this context nutritionists often highlight the importance of fish for human nutrition because of their protein and fatty acid composition. In order to stimulate utilisation and consumption of fish species by unusual target groups such as children, the aim of this research was to formulate and to evaluate shelf-life and nutritional values of fish preparations stored in modified atmosphere packaging (MAP). Fish species used for trail were Trachurus trachurus and Oncorhynchus mykiss fished and farmed in Basilicata region respectively...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462205/production-of-farmstead-lactose-free-pecorino-di-osilo-and-ricotta-cheeses-from-sheep-s-milk
#14
Luisa Pulinas, Carlo Spanu, Ilenia Idda, Ignazio Ibba, Gavino Nieddu, Salvatore Virdis, Christian Scarano, Francesca Piras, Nadia Spano, Gavino Sanna, Enrico Pietro Luigi De Santis
The present work was aimed to define and validate farmstead production of lactose-free Pecorino di Osilo cheese, fresh ricotta cheese, and salted and smoked ricotta cheese (Ricotta mustia). The enzymatic activity of the commercial preparation containing lactase (1.1 g/mL), preliminarily tested using a spectrophotometric titration, showed activity equal to 4950±40 neutral lactase unit/g. The amount of lactase required to obtain the lactose-free milk was then established in triplicate laboratory trials, by adding the enzyme at concentrations of 0...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462204/analysis-of-provincial-and-municipal-regulations-governing-crustacean-welfare-in-italy
#15
Gaetano Liuzzo, Roberto Rossi, Federica Giacometti, Giulia Mescolini, Silvia Piva, Andrea Serraino
The trade in live crustaceans implies keeping these animals alive after capture and/or farming until purchase by the final consumer. Regarding animal welfare, the European Union includes cephalopods in Directive 2010/63/EU on the protection of animals used for scientific purposes, but there are no further regulations on crustaceans in EU legislation. The present study analysed the provisions of Italian municipal regulations on animal welfare applicable to crustaceans. Only 62 of the 110 municipal websites of the provincial capitals reported a regulation safeguarding animal welfare...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462203/analysis-of-process-factors-of-dry-fermented-salami-to-control-listeria-monocytogenes
#16
Enrico Novelli, Lucia Dal Santo, Stefania Balzan, Barbara Cardazzo, Dino Spolaor, Angiolella Lombardi, Lisa Carraro, Luca Fasolato
Challenge tests are a clear opportunity for manufacturers interested in the evaluation of their management system with the aim to reduce the spread of foodborne pathogens. This is a main concern especially in ready-to-eat food in relation to the risk associated with Listeria monocytogenes. For small and medium-scale food industry the manufacturing practices and products formulation are characterised by a wider variability and poor repeatability. The use of ad hoc challenge test and the comparison among different processing systems are strongly required...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462202/genuine-and-natural-the-opinion-of-teen-consumers
#17
Stefania Balzan, Luca Fasolato, Barbara Cardazzo, Cristiana Penon, Enrico Novelli
Food packaging frequently reports the terms natural, 100% natural or similar. Often these indications induce consumers to purchase those products that are considered healthier and fresher. The overall goal of this study was to assess what teen consumers perceive to be genuine and natural foods. A questionnaire was distributed to the students of some high schools (lyceum, technical and professional institutes). It was completed by 349 females and 314 males, with an average age of 17.6 years. Respondents are quite interested in the information on recipes, diet, beauty and food safety; websites were important information retrieval tools...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462201/salting-by-vacuum-brine-impregnation-in-nitrite-free-lonza-effect-on-enterobacteriaceae
#18
Annalisa Serio, Clemencia Chaves-López, Chiara Rossi, Paola Pittia, Marco Dalla Rosa, Antonello Paparella
Lonza is a traditional Italian meat product made from whole pork muscles, which is typically cured by dry salting. In this work, we study the effects of vacuum brine impregnation (VBI) as an alternative salting method on the survival of Enterobacteriaceae, in presence and in absence of nitrites. In comparison with the traditional brining process, VBI contributed to reducing the Enterobacteriaceae population on product surface but induced contamination of the inner muscle tissues. Our results suggest that the species isolated became adapted to processing conditions, and salt tolerance was species- or strain-dependent...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299291/antibacterial-activity-of-metabolites-products-of-vibrio-alginolyticus-isolated-from-sponge-haliclona-sp-against-staphylococcus-aureus
#19
Happy Nursyam
The objective of this study was to investigate the antibacterial activity of primary and secondary metabolites from Vibrio alginoliticus isolated from sponge Haliclona sp. against Staphylococcus aureus. A descriptive method was used in this research. The antibacterial activity was analysed by paper disk method. The results showed that the primary metabolites produced by Vibrio alginoliticus that is in symbiosis with sponge Haliclona sp. were able to effectively inhibit Staphylococcus aureus growth with an inhibition zone diameter of 12...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299290/anti-listeria-activity-of-lactic-acid-bacteria-in-two-traditional-sicilian-cheeses
#20
Maria Luisa Scatassa, Raimondo Gaglio, Cinzia Cardamone, Giusi Macaluso, Luigi Arcuri, Massimo Todaro, Isabella Mancuso
Listeria monocytogenes is a pathogen frequently found in dairy products, and its growth is difficult to control. Bacteriocin-like inhibitory substances (BLIS), produced by lactic acid bacteria (LAB), having proven in vitro anti-Listeria activity, could provide an innovative approach to control L. monocytogenes; however, this application needs to be evaluated in vivo. In this study, twenty LAB strains isolated from different Sicilian dairy environments were tested for control of growth of L. monocytogenes in three different experimental trials...
January 24, 2017: Italian Journal of Food Safety
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