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Italian Journal of Food Safety

Fausto Scoppetta, Marco Sensi, Maria Pia Franciosini, Marinella Capuccella
Antibiotic use in food-producing animals has considerable impact on public health, especially with respect to the development and spread of antibiotic resistance. Pigs represent one of the main species in which antibiotics are frequently used for different purposes. Surveillance of antibiotic consumption and dose appropriateness, through novel approaches based on defined daily doses, is strongly needed to assess farms' antibiotic risk, in terms of spread of antibiotic resistance and possibile presence of residues in meat...
August 16, 2017: Italian Journal of Food Safety
Angela M I Montone, Alessandra De Felice, Roberta Brunetti, Domenico Mollica, Salvatore Capo, Federico Capuano, Achille Guarino, Donatella Nava
Fresh ricotta (whey cheese), owing to its technological characteristics and intrinsic parameters (pH and a(w)), is an excellent substrate for the growth of spoilage and pathogenic microorganisms. On 19 July 2010, Campania buffalo ricotta cheese was assigned to the register of Protected Designations of Origin (PDO) by the European Commission, with EC Reg. 634/2010. It is mainly produced in the provinces of Caserta and Salerno around the rivers Garigliano and Volturno, the plain of the river Sele and the area of Cilento...
August 16, 2017: Italian Journal of Food Safety
Edmondo Ceci, Patrizia Marchetti, Giorgio Samoilis, Stefano Sportelli, Rocco Roma, Roberta Barrasso, Giuseppina Tantillo, Giancarlo Bozzo
The plasmatic cortisol levels of 60 eight-month-old calves (Charolais breed) were measured as stress indicators resulting from two types of slaughter: traditional and religious rite. The plasmatic cortisol levels were evaluated during three different stages of their productive life: during growth, after transport and during slaughter. The thirty calves slaughtered after stunning showed plasmatic cortisol values of 4.85±3.2; 36.36±12.2 and 45.08±14.1 nmol/L, during growth, in the slaughterhouse stables and during exsanguination, respectively...
August 16, 2017: Italian Journal of Food Safety
Elisa Massella, Silvia Piva, Federica Giacometti, Gaetano Liuzzo, Angelo Vittorio Zambrini, Andrea Serraino
Bovine beta casein A1 is one of the most common variants in dairy cattle breeds; it is considered a risk factor in milk intolerance and in other important human diseases, because of the bioactive peptide beta casomorphin-7 (BCM7) produced by raw or processed A1-milk, but not by A2-milk, during digestion. The aim of this study was to perform a cheap and rapid method to investigate beta casein polymorphism in copious animals. The study included 2 dairy farms with a totally of 1230 cows. Beta casein genotypes were estimated evaluating Exon 7 region of bovine beta casein gene (CSN2) by sequences analysis...
August 16, 2017: Italian Journal of Food Safety
Maria Serena Altissimi, Rossana Roila, Raffaella Branciari, Dino Miraglia, David Ranucci, Marisa Framboas, Naceur Haouet
Acrylamide dietary intakes from selected street foods in youth population are estimated. The intake evaluation was carried out by combining levels of acrylamide in food, analytically determined by high performance liquid chromatography, with individual consumption data recorded using a questionnaire applied to a group of 200 students aged 19 to 30. The mean value of acrylamide exposure was recorded to be 0.452 µg/kg bw/day, while the average intakes at 50(th) and 95(th) percentile were 0.350 µg/kg bw/day and 1...
August 16, 2017: Italian Journal of Food Safety
Federica Palma, Frédérique Pasquali, Alex Lucchi, Alessandra De Cesare, Gerardo Manfreda
Listeria monocytogenes is a food-borne pathogen able to survive and grow in different environments including food processing plants where it can persist for month or years. In the present study the discriminatory power of Whole Genome Sequencing (WGS)-based analysis (cgMLST) was compared to that of molecular typing methods on 34 L. monocytogenes isolates collected over one year in the same rabbit meat processing plant and belonging to three genotypes (ST14, ST121, ST224). Each genotype included isolates indistinguishable by standard molecular typing methods...
August 16, 2017: Italian Journal of Food Safety
Mirjam Hochreutener, Claudio Zweifel, Sabrina Corti, Roger Stephan
To assess the antimicrobial effect of a commercial steam-vacuuming system newly implemented after slaughtering, 105 cattle carcasses were examined for total viable counts (TVC) at four different areas. Before steam vacuuming, mean TVC of the excision samples were comparable at the perineal area and brisket (3.0-3.1 log CFU cm(-2)) or the hind leg and shoulder (2.6-2.7 log CFU cm(-2)). Steam vacuuming reduced mean TVC by 0.9, 0.7, 0.6, and 0.4 log CFU cm(-2) at the perineal area, hind leg, shoulder, and brisket, respectively...
August 16, 2017: Italian Journal of Food Safety
Cesare Ciccarelli, Angela Marisa Semeraro, Melina Leinoudi, Vittoria Di Trani, Sandra Murru, Piero Capocasa, Elena Ciccarelli, Luca Sacchini
Consumption of bivalve shellfish harvested from water contaminated with sewage pollution presents a risk of human infections and targeting control measures require a good understanding of environmental factors influencing the transport and the fate of faecal contaminants within the hydrological catchments. Although there has been extensive development of regression models, the point of this paper, focused on the relationship between rainfall events and concentrations of Escherichia coli monitored in clams, was the use of a Bayesian approach, by the Bayes Factor...
August 16, 2017: Italian Journal of Food Safety
Koichi Murakami, Tamie Noda, Daisuke Onozuka, Hirokazu Kimura, Shuji Fujimoto
The diversity of pulsed-field profiles (PFPs) within non-typhoidal Salmonella subtypes influences epidemiological analyses of Salmonella outbreaks. Therefore, determining the PFP diversity of each Salmonella serovar is important when evaluating current circulating strains. This study examined the PFP diversity of three important public health Salmonella enterica subspecies enterica serovars, S. Enteritidis (n=177), S. Infantis (n=205), and S. Corvallis (n=90), using pulsed-field gel electrophoresis. Isolates were collected from several sources, primarily from chicken-derived samples, in the Kyushu-Okinawa region of Japan between 1989 and 2005...
August 16, 2017: Italian Journal of Food Safety
Patrizia Centorame, Anna Rita D'Angelo, Federica Di Simone, Romolo Salini, Alessandra Cornacchia, Raffaele Marrone, Aniello Anastasio, Francesco Pomilio
No abstract text is available yet for this article.
August 16, 2017: Italian Journal of Food Safety
Alessandro Giuffrida, Filippo Giarratana, Davide Valenti, Daniele Muscolino, Roberta Parisi, Alessio Parco, Stefania Marotta, Graziella Ziino, Antonio Panebianco
Three data sets concerning the behaviour of spoilage flora of fillets treated with natural preservative substances (NPS) were used to construct a new kind of mathematical predictive model. This model, unlike other ones, allows expressing the antibacterial effect of the NPS separately from the prediction of the growth rate. This approach, based on the introduction of a parameter into the predictive primary model, produced a good fitting of observed data and allowed characterising quantitatively the increase of shelf-life of fillets...
April 13, 2017: Italian Journal of Food Safety
Anna Maria Bazzoni, Alessandro Graziano Mudadu, Giuseppa Lorenzoni, Igor Arras, Antonella Lugliè, Barbara Vivaldi, Valentina Ciccotelli, Giovanna Sanna, Giuseppe Tedde, Salvatore Ledda, Enrico Alesso, Edoardo Marongiu, Sebastiano Virgilio
[This corrects the article on p. 6095 in vol. 5, PMID: 28058244.].
April 13, 2017: Italian Journal of Food Safety
Carlotta Ceniti, Domenico Britti, Adriano Michele Luigi Santoro, Rosanna Musarella, Lucia Ciambrone, Francesco Casalinuovo, Nicola Costanzo
Mastitis is the most frequent and costly disease of lactating animals and is associated with a significant reduction in milk yield, increased cost and culling. Early and specific antibiotic based treatment reduces the severity of the disease. Over the years the extensive use of antimicrobials has led to increase antimicrobial resistance. The present study was designed to investigate the prevalence of microorganisms responsible for mastitis and their antimicrobial resistance pattern. A total of 282 milk samples were collected from different animal species (sheep, cows and goats) with clinical mastitis...
April 13, 2017: Italian Journal of Food Safety
Lia Bardasi, Roberta Taddei, Ilaria Fiocchi, Maria Francesca Pelliconi, Mattia Ramini, Elena Toschi, Giuseppe Merialdi
During the years 2015-2016, 83 faecal samples were collected at slaughter from pigs reared in farms located in Central-Northern Italy. During the years 2014-2016 a total of 562 pork products [465 not-readyto-eat (NRTE) and 97 ready-to-eat (RTE) products] were collected from retail outlets, large retailers and processing plants. The samples were analysed according to ISO TS 13136:2012. Out of 83 swine faecal samples, 77 (92.8%) resulted stx-positive by real time polymerase chain reaction (PCR), 5 stx2+ and 1 stx1+ Shiga toxin-producing Escherichia coli (STEC) strains were isolated...
April 13, 2017: Italian Journal of Food Safety
Giuseppe Carrabs, Giorgio Smaldone, Leonardo Carosielli, Mariagrazia Girasole, Marco Iammarino, Eugenio Chiaravalle
In Europe sulfur dioxide (SO2) and sulfites in foods and beverages at concentrations of more than 10 mg kg(-1) or 10 mg L(-1) expressed as SO2 equivalents are subject to mandatory labelling. In fresh meats the addition of sulfites is not admitted because of their unlawful use to give the product a more attractive appearance. Aim of the study was to detect sulfites in meat product sampled in 19 commercial shops of Lazio Region. In n=12 samples, sulfites were present at different concentrations and no indication for them was reported on the label...
April 13, 2017: Italian Journal of Food Safety
Serena Santonicola, Maria Francesca Peruzy, Mariagrazia Girasole, Nicoletta Murru, Maria Luisa Cortesi, Raffaelina Mercogliano
Pre-slaughter stress can result in variations in the glycogen storage and metabolic changes of muscle, responsible for quality poultry meat. Aim of this study was to investigate, as pre-slaughter stress markers and quality meat, physicochemical (pH), biochemical (muscle glycogen content), and chemical (super oxides free radicals) parameters. The carcass quality, as incidence of individual carcass defects, was also evaluated. Twenty broilers were processed with two different electrical stunning: high (250 Hz; 640 mA; 60V) (Lot C or control) and low (150 Hz; 360 mA; 60 V) (Lot A) frequency and intensity, using sinusoidal alternating current...
April 13, 2017: Italian Journal of Food Safety
Nicoletta Murru, Giuseppe Blaiotta, Maria Francesca Peruzy, Serena Santonicola, Raffaelina Mercogliano, Maria Aponte
A screening for oxalate degrading abilities was initially carried on within Lactic Acid Bacteria cultures of different food origin. Seventy-nine strains were drop-inoculated onto MRS agar plates containing calcium oxalate. By comparing colonies diameters, 31 strains were used to inoculate, in parallel, MRS and MRS modified by sodium oxalate addition. Differences in the strains' growth were assessed by colony forming unit counts. For two strains, the growth in oxalate enriched medium was significantly higher; while, for eleven strains an opposite behaviour was recorded...
April 13, 2017: Italian Journal of Food Safety
Olga Lobos, Andrea Barrera, Carlos Padilla
The objective was to analyse the antibacterial ability of members of the intestinal microbiota of Oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. Bacterial colonies from an intestinal portion of the 20 specimens of O. mykiss were obtained in different culture media. Several of the colonies showed antibacterial action on different target strains. The bacterial species with the highest antagonistic capacity were Hafnia alvei and Lactococcus lactis and the more susceptible target strains were Aeromonas hydrophila, Listeria monocytogenes and Escherichia coli...
April 13, 2017: Italian Journal of Food Safety
Giovanni Ferrazzi, Vera Ventura, Sabrina Ratti, Claudia Balzaretti
Italy, with a cultivated area of 218,000 ha, is a European leader of rice production. In particular Lombardy region accounts for 40% of total rice cultivation and the case study in object accounts for 3.2% of Lombardy total rice area (2773 ha). Starting from 2012, through a regional project titled Buono, Sano e Vicino (good, healthy and close), Riso e Rane rural district supported local rice farmers in developing innovation in rice production and promoting an alternative supply chain to increase farmers bargaining power and promote new market strategies...
April 13, 2017: Italian Journal of Food Safety
Celestina Mascolo, Marina Ceruso, Paolo Sordino, Giuseppe Palma, Aniello Anastasio, Tiziana Pepe
Previous studies showed that fish mitochondrial DNA (mtDNA) is set up by a closed circular molecule of 16-17 kilobases (kb), comprising 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), 13 protein-coding genes and 2 non-coding regions. The analysis of single mtDNA genes, such as Cytb, COI, 16S and 12S, or short segment of them, has been widely used against species substitution in both fresh and processed fish products. The analysis of the complete mitochondrial genome of fishery products allows to better study and characterise fish species...
April 13, 2017: Italian Journal of Food Safety
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