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Italian Journal of Food Safety

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https://www.readbyqxmd.com/read/29732334/high-microbial-loads-found-in-minimally-processed-sliced-mushrooms-from-italian-market
#1
Haiyang Jiang, Dino Miraglia, David Ranucci, Domizia Donnini, Rossana Roila, Raffaella Branciari, Cheng Li
There is an increased consumer interest in minimally processed vegetables that has led to the development of products, such as pre-cut sliced mushrooms. Few data are available on the hygienic condition and the presence of foodborne pathogens in such products. Therefore, the current study aimed to evaluate the safety and hygienic characteristics of both ready-to-eat and ready-to-cook, pre-cut sliced mushrooms obtained from a local Italian market. For the evaluation of the hygienic condition, the aerobic mesophilic bacteria, aerobic psychrotrophic bacteria and Escherichia coli enumerations were performed...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732333/quality-assessment-of-zeus-faber-peter-s-fish-ovaries-regularly-commercialized-for-human-consumption
#2
Filippo Giarratana, Graziella Ziino, Valerio D'Andrea, Antonio Panebianco, Alessandro Giuffrida
In the last few years, the consumption of fish eggs has increased rapidly, finding widespread use also in mass catering. This increase has involved also those of the Peter's fish ( Zeus faber ). Females of this species, by their reproductive characteristics, have highly developed gonads in different periods of the year, making the raw material easy to find. The aim of the present study was to perform a quality assessment of Zeus faber ovaries regularly commercialized for human consumption. A total number of 34 samples, divided in fresh (11) and frozen (23), were processed for microbiological characterization, parasitological and histological evaluations...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732332/case-report-of-a-pustular-dermatitis-outbreak-in-sheep-clinical-and-food-safety-considerations
#3
Mariana Roccaro, Silvia Piva, Alessandra Scagliarini, Federica Giacometti, Andrea Serraino, Giuseppe Merialdi, Matteo Frasnelli, Angelo Romano, Alberto Bellio, Lucia Decastelli, Angelo Peli
The objective of this report is to describe an outbreak of pustular dermatitis in a flock of about 200 sheep, its clinical evolution and food safety implications. The onset of the symptoms was sudden and the lesions spread very quickly from ewe to ewe, so that in about 3 days almost all of the lactating sheep were stricken. Pustules from 5 different animals, six milk samples, two cheese samples, teat cup samples from the milking machine and farmer's hands were analysed. A pure culture of Staphylococcus aureus , producing staphylococcal enterotoxin (SE) C, was isolated from pustules...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732331/contribution-of-natural-milk-culture-to-microbiota-safety-and-hygiene-of-raw-milk-cheese-produced-in-alpine-malga
#4
Rosaria Lucchini, Barbara Cardazzo, Lisa Carraro, Michele Negrinotti, Stefania Balzan, Enrico Novelli, Luca Fasolato, Franco Fasoli, Giovanni Farina
Processing of alpine milk in malga farms is carried out under conditions that can favor contamination by coliforms, coagulase-positive staphylococci, or pathogens such as Listeria monocytogenes . With the aim to improve the hygienic characteristics and safety of cheese produced in four malga farms the use of lyophilized Natural Milk Culture prepared with selected strains was tested.. Two cheesemaking tests were carried out in the same day always starting from the same milk: in the first case following the malga recipe that uses either Natural Whey Culture or without the addition of a starter, in the second one using a Natural Milk Culture...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732330/-praedicere-possumus-an-italian-web-based-application-for-predictive-microbiology-to-ensure-food-safety
#5
Pierluigi Polese, Manuela Del Torre, Mara Lucia Stecchini
The use of predictive modelling tools, which mainly describe the response of microorganisms to a particular set of environmental conditions, may contribute to a better understanding of microbial behaviour in foods. In this paper, a tertiary model, in the form of a readily available and userfriendly web-based application Praedicere Possumus (PP) is presented with research examples from our laboratories. Through the PP application, users have access to different modules, which apply a set of published models considered reliable for determining the compliance of a food product with EU safety criteria and for optimising processing throughout the identification of critical control points...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732329/-salmonella-brandenburg-in-the-pork-chain-in-italy-genetic-comparison-with-the-human-isolates
#6
Silvia Bonardi, Marina Morganti, Giovanni Pupillo, Franco Brindani
Salmonella Brandenburg ranked 16th among the serovars responsible for human infections in EU in 2015 and it was found to be associated with swine. In Emilia- Romagna and Lombardy regions of northern Italy, S. Brandenburg was isolated from mesenteric lymph nodes, fecal matter, carcasses and conveyor belts at pig slaughterhouses in 2014 and 2015. In the same area, S. Brandenburg was detected in pork salami in 2015. In the present study, 12 isolates of S. Brandenburg recovered from the pork food-chain were typed by Xba I PFGE and their three profiles were compared to all human S ...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732328/spectrophotometric-techniques-for-the-characterization-of-strains-involved-in-the-blue-pigmentation-of-food-preliminary-results
#7
Luca Fasolato, Nadia Andrea Andreani, Roberta De Nardi, Giulia Nalotto, Lorenzo Serva, Barbara Cardazzo, Stefania Balzan, Lisa Carraro, Federico Fontana, Enrico Novelli
Near infrared spectroscopy (NIRs) and ultraviolet visible spectroscopy (UV-vis) have been investigated as rapid techniques to characterize foodborne bacteria through the analysis of the spectra of whole cells or microbial suspensions. The use of spectra collected from broth cultures could be used as a fingerprint for strain classification using a combined polyphasic approach. The aim of this study was to evaluate the feasibility of NIRs and UV-vis for the characterization of blue strains belonging to the Pseudomonas fluorescens group...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732327/microbiological-profile-of-chicken-carcasses-a-comparative-analysis-using-shotgun-metagenomic-sequencing
#8
Alessandra De Cesare, Federica Palma, Alex Lucchi, Frederique Pasquali, Gerardo Manfreda
In the last few years metagenomic and 16S rRNA sequencing have completly changed the microbiological investigations of food products. In this preliminary study, the microbiological profile of chicken carcasses collected from animals fed with different diets were tested by using shotgun metagenomic sequencing. A total of 15 carcasses have been collected at the slaughetrhouse at the end of the refrigeration tunnel from chickens reared for 35 days and fed with a control diet (n=5), a diet supplemented with 1500 FTU/kg of commercial phytase (n=5) and a diet supplemented with 1500 FTU/kg of commercial phytase and 3g/kg of inositol (n=5)...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732326/information-management-and-ante-mortem-inspection-procedures-for-the-emerging-diseases-control-experiences-acquired-in-the-epidemiological-surveillance-of-bluetongue-and-lumpy-skin-disease
#9
Alessandra Corradini, Marcello Trevisani, Geremia Dosa, Anna Padovani
The spread of exotic, emerging and reemerging diseases, has become, in the last years, one of the most important threats to the animal productions and public health, representing a new challenge for the European Community. In a global-market framework, where trade and contacts between countries are simplified, effective and well-developed surveillance systems are necessary. Multiple factors are, in fact, associated with the emergence of new, known or exotic diseases in this new economic panorama and for these reasons controls on animal imports, traceability and timeliness detection of infected animals should be considered the basis of a sound surveillance...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732325/microbiological-evaluation-of-ready-to-eat-iceberg-lettuce-during-shelf-life-and-effectiveness-of-household-washing-methods
#10
Daniela Bencardino, Luca Agostino Vitali, Dezemona Petrelli
The aim of this study was to assess the microbiological quality of ready-to-eat (RTE) iceberg lettuce. Our investigation was based on the consumption tendency of university students considered a target market for this product. A total of 78 RTE samples were collected from chain supermarkets and analysed for the enumeration of aerobic mesophilic count (AMC), Escherichia coli and the detection of Salmonella spp. and Listeria monocytogenes . All samples were negative for the presence of pathogens. The mean value of AMC at the beginning, in the middle and after the expiration date was: 6...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732324/retrospective-study-on-the-hygienic-quality-of-fresh-ricotta-cheeses-produced-in-sicily-italy
#11
Maria Luisa Scatassa, Isabella Mancuso, Sonia Sciortino, Giusi Macaluso, Marisa Palmeri, Luigi Arcuri, Massimo Todaro, Cinzia Cardamone
The present work was carried out to investigate the microbiological profile of Sicilian ewes' ricotta cheeses during fifteen years of investigations (2002-2016). The samples were collected between those conferred to the Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi) Adelmo Mirri , Palermo (Italy), by the competent authority during official control, by food business operator in HACCP systems and in research projects. Enterobacteriaceae, Escherichia coli and coagulase - positive staphylococci (CPS) were found only in some samples...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732323/analysis-of-the-causes-of-the-seizure-and-destruction-of-carcasses-and-organs-in-a-slaughterhouse-in-central-italy-in-the-2010-2016-period
#12
Margherita Ceccarelli, Elisa Leprini, Paola Sechi, Maria Francesca Iulietto, Luca Grispoldi, Enzo Goretti, Beniamino Terzo Cenci-Goga
Meat safety and hygiene are fundamental issues for producers and endusers. To achieve these goals, the inspection of carcasses and organs at slaughter is critical. The results of post-mortem inspection are the basis for planning preventive measures against consumer risks and for limiting economic losses. In this retrospective study, the causes of the seizure and destruction of carcasses and organs were analysed at a slaughterhouse in central Italy from 2010 to 2016. 436,646 slaughtered animals were taken into consideration, for a total of 61,799 seizures (73...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732322/a-case-study-on-the-labeling-of-bottarga-produced-in-sardinia-from-ovaries-of-grey-mullets-mugil-cephalus-and-mugil-capurrii-caught-in-eastern-central-atlantic-coasts
#13
Pierluigi Piras, Francesco Sardu, Domenico Meloni, Maria Vittoria Riina, Chiara Beltramo, Pier Luigi Acutis
The aim of this case study is to show how traditional and molecular methods can be employed to identify the Mugilidae species currently used in Sardinia (Italy) to produce the traditional bottarga for the processing of their ovaries. A total of six specimens of Mugil cephalus (n=3) and Mugil capurrii (n=3) were subjected to external morphology and meristic measurements. Subsequently, tissue samples of white muscle and ovaries from three individuals per species were underwent PCR-sequencing assay of mitochondrial DNA cytochrome oxidase subunit I ( COI )...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29732321/characterization-of-non-typhoidal-salmonella-enterica-strains-of-human-origin-in-central-and-southern-italy
#14
Yolande T R Proroga, Federico Capuano, Rosanna Capparelli, Stefano Bilei, Mariano Bernardo, Maria Pia Cocco, Rosalba Campagnuolo, Vincenzo Pasquale
Non-typhoidal Salmonella enterica infection is a significant public health problem worldwide. The aim of this study was to characterize Salmonella enterica strains isolated from human specimens in central and southern Italy, for epidemiological studies. One hundred and fifty S. enterica strains were serotyped. Isolates were tested for their antimicrobial susceptibility, by disk diffusion method. The molecular characterizations, based on PCR, were carried out for the detection of invA gene and other virulence elements and phage marker genes...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29564242/analysis-of-a-poultry-slaughter-process-influence-of-process-stages-on-the-microbiological-contamination-of-broiler-carcasses
#15
Denise Althaus, Claudio Zweifel, Roger Stephan
In a large-scale Swiss poultry abattoir, a microbiological process analysis of broiler carcasses was performed. At each selected process stage (scalding, plucking, evisceration, washing, and chilling), 90 carcasses from 30 flocks were sampled and examined for Campylobacter , Salmonella , Escherichia coli , Enterobacteriaceae, and extended-spectrum b-lactamases-producing Enterobacteriaceae. With regard to Campylobacter counts on carcasses, plucking tended to slightly increase the results (on average by 0.4 log CFU/g), whereas mean counts from plucked and chilled carcasses were comparable (3...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29564241/yessotoxin-detection-in-bivalve-molluscs-a-case-study-from-coastal-mussel-farms-sardinia-italy
#16
Alessandro Graziano Mudadu, Giuseppa Lorenzoni, Anna Maria Bazzoni, Riccardo Bazzardi, Giuseppe Tedde, Igor Arras, Giovanna Sanna, Cinzia Santucciu, Edoardo Marongiu, Sebastiano Virgilio
This work reports the first communication relating to the presence of yessotoxins in Mytilus galloprovincialis from coastal mussel farms (Sardinia, western Mediterranean) detected during 2008 and 2013 through a monitoring programme. The paper emphasizes how the changes both in yessotoxin permitted limits and used methods, established by legislation, have influenced the interpretation of the obtained results. Consequently, the samples that resulted negative during 2008 would have been positive until August 2013 and negative from September 2013 up to now, and the samples that were positive in 2013 would have been positive in 2008 and negative nowadays, according to Regulation currently in force...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29564240/preliminary-study-on-the-inactivation-of-anisakid-larvae-in-baccal%C3%A3-prepared-according-to-traditional-methods
#17
Giorgio Smaldone, Raffaele Marrone, Giuseppe Palma, Paolo Sarnelli, Aniello Anastasio
The European Food Safety Authority stated that many traditional marinating and cold smoking methods are not sufficient to kill A. simplex and asked to evaluate alternative treatments for killing viable parasites in fishery . Baccalà is a well-liked traditional product. The aim of study was to evaluate the effectiveness of the salting process on the inactivation of nematodes of the genus Anisakis in naturally infected Baccalà fillets. N. 19 fillets, subjected to a dual salting process (brine and dry salting) were analyzed...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29564239/prevalence-and-characterisation-of-shigatoxigenic-escherichia-coli-isolated-from-beef-cattle-fed-with-prebiotics
#18
Luca Grispoldi, Filippo Bertero, Serena Franceschini, Francesco Mastrosimone, Paola Sechi, Maria Francesca Iulietto, Margherita Ceccarelli, Beniamino Terzo Cenci-Goga
Ten Holstein Friesian calves were divided into two groups of five: one group was given prebiotics in their food, while the other group served as the control group. Every two weeks from birth up to 18 months, samples of feces were taken from the rectal ampulla to determine the concentration of E. coli . At each sampling session, three aliquots per sample were collected. The arithmetic mean was calculated and all values (converted into logs) were analysed with GraphPad InStat for analysis of variance, followed by the Tukey-Kramer test...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29564238/antimicrobial-activity-of-four-essential-oils-against-pigmenting-pseudomonas-fluorescens-and-biofilmproducing-staphylococcus-aureus-of-dairy-origin
#19
Francesca Pedonese, Filippo Fratini, Luisa Pistelli, Federica Maria Porta, Pierluigi Di Ciccio, Roberto Fischetti, Barbara Turchi, Roberta Nuvoloni
Essential oils (EOs) are mixtures of secondary metabolites of plant origin with many useful properties, among which the antimicrobial activity is also of interest for the food industry. EOs can exert their antimicrobial potential both directly, in food products and active packaging, and indirectly, as sanitizing and anti-biofilm agents of food facility surfaces. Aim of this research was to evaluate the antimicrobial activity of four EOs (bergamot, cinnamon, manuka and thyme) against Pseudomonas fluorescens and Staphylococcus aureus isolated from milk and dairy products...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29564237/indicators-of-protein-spoilage-in-fresh-and-defrosted-crustaceans-and-cephalopods-stored-in-domestic-condition
#20
Serena Altissimi, Maria Lucia Mercuri, Marisa Framboas, Mauro Tommasino, Stefania Pelli, Ferdinando Benedetti, Sara Di Bella, Naceur Haouet
In relation to consumer demand, crustaceans and cephalopods are sold as both fresh and defrosted. It is well known that total volatile basic nitrogen (TVB-N) and volatile amine values, especially, biogenic amines and biogenic amine index, are expression of freshness of fish products, but there is a lack of knowledge of their acceptability limits, for crustaceans and cephalopods. In order to assess these limits, real-time shelf life tests were carried out, relating the results of TVB-N, biogenic amines and BAI to the sensory evaluation of crustaceans and cuttlefishes, both fresh and defrosted...
October 20, 2017: Italian Journal of Food Safety
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