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Italian Journal of Food Safety

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https://www.readbyqxmd.com/read/28462207/toxoplasma-gondii-and-pre-treatment-protocols-for-polymerase-chain-reaction-analysis-of-milk-samples-a-field-trial-in-sheep-from-southern-italy
#1
Alice Vismarra, Elena Barilli, Maura Miceli, Carlo Mangia, Cristina Bacci, Franco Brindani, Laura Kramer
Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii. Ingestion of raw milk has been suggested as a risk for transmission to humans. Here the authors evaluated pre-treatment protocols for DNA extraction on T. gondii tachyzoite-spiked sheep milk with the aim of identifying the method that resulted in the most rapid and reliable polymerase chain reaction (PCR) positivity. This protocol was then used to analyse milk samples from sheep of three different farms in Southern Italy, including real time PCR for DNA quantification and PCR-restriction fragment length polymorphism for genotyping...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462206/formulation-and-shelf-life-of-fish-burgers-served-to-preschool-children
#2
Giorgio Smaldone, Raffaele Marrone, Tiziana Zottola, Lucia Vollano, Giulio Grossi, Maria Luisa Cortesi
Consumer is very careful about healthiness; in this context nutritionists often highlight the importance of fish for human nutrition because of their protein and fatty acid composition. In order to stimulate utilisation and consumption of fish species by unusual target groups such as children, the aim of this research was to formulate and to evaluate shelf-life and nutritional values of fish preparations stored in modified atmosphere packaging (MAP). Fish species used for trail were Trachurus trachurus and Oncorhynchus mykiss fished and farmed in Basilicata region respectively...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462205/production-of-farmstead-lactose-free-pecorino-di-osilo-and-ricotta-cheeses-from-sheep-s-milk
#3
Luisa Pulinas, Carlo Spanu, Ilenia Idda, Ignazio Ibba, Gavino Nieddu, Salvatore Virdis, Christian Scarano, Francesca Piras, Nadia Spano, Gavino Sanna, Enrico Pietro Luigi De Santis
The present work was aimed to define and validate farmstead production of lactose-free Pecorino di Osilo cheese, fresh ricotta cheese, and salted and smoked ricotta cheese (Ricotta mustia). The enzymatic activity of the commercial preparation containing lactase (1.1 g/mL), preliminarily tested using a spectrophotometric titration, showed activity equal to 4950±40 neutral lactase unit/g. The amount of lactase required to obtain the lactose-free milk was then established in triplicate laboratory trials, by adding the enzyme at concentrations of 0...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462204/analysis-of-provincial-and-municipal-regulations-governing-crustacean-welfare-in-italy
#4
Gaetano Liuzzo, Roberto Rossi, Federica Giacometti, Giulia Mescolini, Silvia Piva, Andrea Serraino
The trade in live crustaceans implies keeping these animals alive after capture and/or farming until purchase by the final consumer. Regarding animal welfare, the European Union includes cephalopods in Directive 2010/63/EU on the protection of animals used for scientific purposes, but there are no further regulations on crustaceans in EU legislation. The present study analysed the provisions of Italian municipal regulations on animal welfare applicable to crustaceans. Only 62 of the 110 municipal websites of the provincial capitals reported a regulation safeguarding animal welfare...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462203/analysis-of-process-factors-of-dry-fermented-salami-to-control-listeria-monocytogenes
#5
Enrico Novelli, Lucia Dal Santo, Stefania Balzan, Barbara Cardazzo, Dino Spolaor, Angiolella Lombardi, Lisa Carraro, Luca Fasolato
Challenge tests are a clear opportunity for manufacturers interested in the evaluation of their management system with the aim to reduce the spread of foodborne pathogens. This is a main concern especially in ready-to-eat food in relation to the risk associated with Listeria monocytogenes. For small and medium-scale food industry the manufacturing practices and products formulation are characterised by a wider variability and poor repeatability. The use of ad hoc challenge test and the comparison among different processing systems are strongly required...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462202/genuine-and-natural-the-opinion-of-teen-consumers
#6
Stefania Balzan, Luca Fasolato, Barbara Cardazzo, Cristiana Penon, Enrico Novelli
Food packaging frequently reports the terms natural, 100% natural or similar. Often these indications induce consumers to purchase those products that are considered healthier and fresher. The overall goal of this study was to assess what teen consumers perceive to be genuine and natural foods. A questionnaire was distributed to the students of some high schools (lyceum, technical and professional institutes). It was completed by 349 females and 314 males, with an average age of 17.6 years. Respondents are quite interested in the information on recipes, diet, beauty and food safety; websites were important information retrieval tools...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28462201/salting-by-vacuum-brine-impregnation-in-nitrite-free-lonza-effect-on-enterobacteriaceae
#7
Annalisa Serio, Clemencia Chaves-López, Chiara Rossi, Paola Pittia, Marco Dalla Rosa, Antonello Paparella
Lonza is a traditional Italian meat product made from whole pork muscles, which is typically cured by dry salting. In this work, we study the effects of vacuum brine impregnation (VBI) as an alternative salting method on the survival of Enterobacteriaceae, in presence and in absence of nitrites. In comparison with the traditional brining process, VBI contributed to reducing the Enterobacteriaceae population on product surface but induced contamination of the inner muscle tissues. Our results suggest that the species isolated became adapted to processing conditions, and salt tolerance was species- or strain-dependent...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299291/antibacterial-activity-of-metabolites-products-of-vibrio-alginolyticus-isolated-from-sponge-haliclona-sp-against-staphylococcus-aureus
#8
Happy Nursyam
The objective of this study was to investigate the antibacterial activity of primary and secondary metabolites from Vibrio alginoliticus isolated from sponge Haliclona sp. against Staphylococcus aureus. A descriptive method was used in this research. The antibacterial activity was analysed by paper disk method. The results showed that the primary metabolites produced by Vibrio alginoliticus that is in symbiosis with sponge Haliclona sp. were able to effectively inhibit Staphylococcus aureus growth with an inhibition zone diameter of 12...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299290/anti-listeria-activity-of-lactic-acid-bacteria-in-two-traditional-sicilian-cheeses
#9
Maria Luisa Scatassa, Raimondo Gaglio, Cinzia Cardamone, Giusi Macaluso, Luigi Arcuri, Massimo Todaro, Isabella Mancuso
Listeria monocytogenes is a pathogen frequently found in dairy products, and its growth is difficult to control. Bacteriocin-like inhibitory substances (BLIS), produced by lactic acid bacteria (LAB), having proven in vitro anti-Listeria activity, could provide an innovative approach to control L. monocytogenes; however, this application needs to be evaluated in vivo. In this study, twenty LAB strains isolated from different Sicilian dairy environments were tested for control of growth of L. monocytogenes in three different experimental trials...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299289/occurrence-of-nematodes-of-the-genus-anisakis-in-mediterranean-and-atlantic-fish-marketed-in-sardinia
#10
Daniele Casti, Christian Scarano, Maria Cristina Piras, Paolo Merella, Sonia Muglia, Francesca Piras, Giovanni Garippa, Carlo Spanu, Enrico Pietro Luigi De Santis
Anisakiasis is a gastrointestinal fish-borne zoonosis caused by the ingestion of third stage larvae of the genus Anisakis. Between January and December 2013, 1112 specimens of four commercial fish species (Engraulis encrasicolus, Merluccius merluccius, Scomber colias and Trachurus mediterraneus) marketed in Sardinia (Italy) were examined for Anisakis sp. The overall prevalence of Anisakis spp larvae was 39.9%, all morphologically identified as Type I. Scomber colias showed the highest prevalence (100%), followed by M...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299288/food-safety-and-sustainable-nutrition-workshops-educational-experiences-for-primary-school-children-in-turin-italy
#11
Amaranta Traversa, Daniela Adriano, Alberto Bellio, Daniela Manila Bianchi, Silvia Gallina, Clara Ippolito, Angelo Romano, Paola Durelli, Andrea Pezzana, Lucia Decastelli
European control and prevention policies are focused to guarantee a high level of protection of consumers' health. Food-borne diseases as obesity, diabetes, food allergy, and food-borne outbreaks are increasing. To prevent food-borne diseases, it is fundamental to involve consumers, in particular children, in educational experiences aimed to learn the proper behaviours to be applied. In this context, we designed and performed 5 educational workshops about food safety, hidden allergens in food and nutrition aimed to involve children attending primary and summer school...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28299287/low-prevalence-of-salmonella-enterica-in-cull-dairy-cattle-at-slaughter-in-northern-italy
#12
Silvia Bonardi, Ilaria Bruini, Rossella Magnani, Nicolò Cannistrà, Franco Brindani
In order to evaluate Salmonella carrier status of cull dairy cattle at slaughter, 125 animals were randomly selected during the period February-May 2016. Dairy cows were reared in 89 farms located in two regions of Northern Italy (Lombardy and Emilia-Romagna regions), where bovine milk is primarily used for Parmigiano-Reggiano cheese and Grana Padano cheese production. Samples were collected by swabbing a 400-cm(2) area of the brisket hide and by rectoanal mucosal swabs. They were tested following the reference ISO 6579 method and the isolates were serotyped following the Kauffmann-White-Le Minor scheme and genotyped by XbaI PFGE...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28217561/characterisation-of-the-thermostable-protease-aprx-in-strains-of-pseudomonas-fluorescens-and-impact-on-the-shelf-life-of-dairy-products-preliminary-results
#13
Nadia Andrea Andreani, Lisa Carraro, Luca Fasolato, Stefania Balzan, Rosaria Lucchini, Enrico Novelli, Barbara Cardazzo
Bacterial proteases are involved in food spoilage and shelf-life reduction. Among the bacterial proteases, a predominant role in spoilage of dairy products seems to be played by the thermostable metallo-protease AprX, which is produced by various strains of Pseudomonas fluorescens. Differences in AprX enzyme activity among different strains were highlighted, but the most proteolytic strains were not identified. In this study, the presence of the aprX gene was evaluated in 69 strains isolated from food matrices and 18 reference strains belonging to the P...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28217560/core-shell-in-liquid-chromatography-application-for-determining-sulphonamides-in-feed-and-meat-using-conventional-chromatographic-systems
#14
Antonio Armentano, Simona Summa, Sonia Lo Magro, Pasquale D'Antini, Carmen Palermo, Marilena Muscarella
A C18 column packed with core-shell particles was used for the chromatographic separation of sulphonamides in feed and meat by a conventional high performance liquid chromatography system coupled with a diode array detector. Two analytical methods, already used in our laboratory, have been modified without any changes in the extraction and clean-up steps and in the liquid chromatography instrumentation. Chromatographic conditions applied on a traditional 5-µm column have been optimized on a column packed with 2...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058252/risk-assessment-in-the-recovery-of-food-for-social-solidarity-purposes-preliminary-data
#15
Vesna Milicevic, Giampaolo Colavita, Marta Castrica, Sabrina Ratti, Antonella Baldi, Claudia M Balzaretti
The most recent study, conducted by Politecnico of Milan, on food surplus management in Italy shows that in the Italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. During 2015, the charitable organizations (COs) belonging to the Italian Food Bank Network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. The main supplying sources of the Banco Alimentare Network are: food industries, organized large-scale retail trade and collective catering service...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058251/evaluation-of-the-antibacterial-activity-of-bergamot-essential-oils-on-different-listeria-monocytogenes-strains
#16
Stefania M Marotta, Filippo Giarratana, Alessio Parco, Domenico Neri, Graziella Ziino, Alessandro Giuffrida, Antonio Panebianco
Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight Listeria monocytogenes strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for L. monocytogenes strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058250/effect-of-a-phenolic-extract-from-olive-vegetation-water-on-fresh-salmon-steak-quality-during-storage
#17
Dino Miraglia, Sonia Esposto, Raffaella Branciari, Stefania Urbani, Maurizio Servili, Simona Perucci, David Ranucci
This study aimed to evaluate the antioxidant and antimicrobial effects of a phenolic extract from olive vegetation water on fresh salmon steaks stored at 4°C under modified atmosphere. Twenty-four salmon steaks were respectively immersed in solutions of the diluted phenolic extract at 1.5 g/L (A), 3 g/L (B), and water only as a control (CTR), packaged within a protective atmosphere (70% carbon dioxide, 25% nitrogen and 5% oxygen) and then stored at 4°C. After 2 h, and 3 and 6 days of storage, the fish samples were analysed for the total viable count, Enterobacteriaceae count, pH, colour (CIE L*a*b* colour system), phenolic composition, atocopherol content, antioxidant activity by 2,2- diphenyl- 1-picrylhydrazyl (DPPH˙) assay, and thiobarbituric reactive substances (TBARS)...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058249/microbiological-and-chemico-physical-shelf-life-and-panel-test-to-evaluate-acceptability-of-liver-mortadella
#18
Erica Tirloni, Simone Stella, Cristian Bernardi, Vittorio Maria Moretti, Carla Bersani, Patrizia Cattaneo
This study aimed to evaluate the shelf life of sliced cooked liver mortadella packaged in MAP (70-85% N2, 15-30% CO2) and stored in refrigeration (4°C) or slight thermal abuse (8°C) for up to 49 days (declared best before date 45 days). The proximate composition, aw nitrites and NaCl content were determined at T0. Weekly, samples were submitted to microbiological [total viable count (TVC), lactic acid bacteria (LAB), Enterobacteriaceae, Escherichia coli, Pseudomonas spp., coagulase positive staphylococci, sulphite reducing clostridia, yeasts and moulds, Listeria monocytogenes and Salmonella spp...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058248/first-multi-year-retrospective-study-on-vibrio-parhaemolyticus-and-vibrio-vulnificus-prevalence-in-ruditapes-philippinarum-harvested-in-sacca-di-goro-italy
#19
Patrizia Serratore, Fabio Ostanello, Pier Luca Passalacqua, Emanuele Zavatta, Giorgia Bignami, Andrea Serraino, Federica Giacometti
The present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of Vibrio spp., and the prevalence of V. parahaemolyticus and V. vulnificus in the Manila clam R. philippinarum harvested in Sacca di Goro, Emilia-Romagna Region, Northern Italy. On the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have been reconsidered for Vibrio spp...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058247/microbiological-contamination-in-three-large-scale-pig-slaughterhouses-in-northern-italy
#20
Pierluigi Di Ciccio, Maria Cristina Ossiprandi, Emanuela Zanardi, Sergio Ghidini, Giancarlo Belluzzi, Alberto Vergara, Adriana Ianieri
The aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaughterhouse (before and during slaughter) were sampled on each sampling day. A single pooled caecal sample was taken on each sampling day. A total of 90 carcasses, 108 environmental samples and 18 caecal samples were collected...
September 20, 2016: Italian Journal of Food Safety
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