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JBRA Assisted Reproduction

Eloísa T Massignam, Maitê Ferreira, Eduardo Sanguinet, Ágata Dupont, Fábio Klamt, Nilo Frantz, Adriana Bos-Mikich
OBJECTIVE: The present study analyzed the quality of bovine ovarian tissue after vitrification in a metal closed chamber, in terms of putative changes in tissue viability (lactate dehydrogenase -LDH- release), anti-oxidant defenses, and redox parameters caused by cryopreservation. METHODS: Small and large fragmented bovine ovarian tissue specimens were vitrified in a metal chamber. After rewarming, tissue samples were fixed or cultured for 48 hours. Glutathione (GSH), protein sulfhydryl content, Total Radical Trapping Antioxidant Potential (TRAP), and lactate dehydrogenase were analyzed immediately after rewarming and after tissue culture...
July 5, 2018: JBRA Assisted Reproduction
Maryam Eftekhar, Ramesh Baradaran Bagheri, Nosrat Neghab, Robabe Hosseinisadat
OBJECTIVE: This study aimed to evaluate the effect of three days of GnRH antagonist pretreatment on the pregnancy outcomes of women with polycystic ovarian syndrome (PCOS) on GnRH antagonist protocols for IVF/ICSI. METHODS: Fifty women with PCOS in the control group received conventional antagonist protocols, starting on day 2 of the cycle. In the pretreatment group (n=38), a GnRH antagonist was administered from day 2 of the menstrual cycle for three days. RESULTS: Controlled ovarian stimulation (COS) duration and gonadotropin dosages were similar in both groups...
July 3, 2018: JBRA Assisted Reproduction
Otoufe Sheikhi, Masoumeh Golsorkhtabaramiri, Sedighe Esmaeilzadeh, Treza Mahouti, Fateme Nadi Heidari
OBJECTIVE: This study set out to investigate the pregnancy outcome of natural cycle regimen versus other endometrial preparation protocols with vitrification thawed blastocyst transfer (VTBT) cycles. METHODS: This control trial study was carried out on 123 women undergoing VTBT. The women were randomly divided into three groups of endometrial preparation before VTBT; 1. Modified natural ovulation cycle with using HCG (n=32) 2. Mild hormonally stimulated cycle by low dose Clomiphene Citrate (n=30) and 3...
June 27, 2018: JBRA Assisted Reproduction
Juliano Augusto Brum Scheffer, Bruno Scheffer, Rafaela Scheffer, Fabio Florencio, Michael Grynberg, Daniel Mendez Lozano
OBJECTIVE: Ovarian reserve evaluation has been the focus of substantial clinical research for several years. This study aimed to examine the associations between markers of ovarian reserve and ovarian response. METHODS: This prospective study included 132 infertile women aged 24-48 years undergoing routine exploration during unstimulated cycles prior to the start of assisted reproductive technology (ART) treatments at our center from July 2015 to January 2017. Descriptive parameters and patient characteristics were reported as mean (SD) or median (range) values depending on the data distribution pattern...
June 27, 2018: JBRA Assisted Reproduction
Layasadat Khorsandi, Ali Akbar Oroojan
OBJECTIVE: To evaluate the hydroethanolic extract toxicity, obtained from Tropaeolum majus L. (TM) on mouse testicular tissue. METHOD: In this experimental study, we used 32 male NMRI mice. The experimental groups received 75, 375 and 750 mg/kg of TM extract, respectively. Twenty-four hours after the last experimental day, serum samples were collected for hormonal measurement. Then, the cauda of epididymis and testis were removed for sperm count and histopathological assessments...
June 27, 2018: JBRA Assisted Reproduction
Emma Adolfsson, Sandra Porath, Anna Nowosad Andershed
OBJECTIVE: To validate a morphokinetic implantation model developed for EmbryoScope on embryos with known outcome, compared to standard morphology in a retrospective single center study. METHODS: Morphokinetic annotation of 768 embryos with known outcome between 2013 -2015; corresponding to 116 D3 fresh embryos, 80 D6 frozen blastocysts, and 572 D5 blastocysts, fresh or frozen. The embryos were ranked by the KIDScore into five classes, KID1-5, and grouped into four classes based on standard morphology...
June 22, 2018: JBRA Assisted Reproduction
Parnaz Borjian Boroujeni, Marjan Sabbaghian, Mehdi Totonchi, Niloofar Sodeifi, Homa Sarkardeh, Azam Samadian, Mohammad Ali Sadighi-Gilani, Hamid Gourabi
OBJECTIVE: Spermatogenesis is a complex process controlled by a plethora of genes. Changes in expression and function of these genes may thus lead to spermatogenic deficiency and male infertility. TEX11, TEX12, TEX14 and TEX15 are germ cell-specific genes expressed in the testis. TEX11, involved in the initiation and maintenance of chromosome synapses in meiotic chromosomes, has been shown to be essential for meiosis and fertility in males. TEX14, a component of intercellular bridges in germ cells, is required for spermatogenesis and fertility...
June 22, 2018: JBRA Assisted Reproduction
Márcia M Carneiro, Ana M Cota, Maria C Amaral, Moisa L Pedrosa, Bruna O Martins, Marcelo H Furtado, Rivia M Lamaita, Marcia C F Ferreira
Breast cancer may affect young women who have not yet completed childbearing. Assisted reproductive technology (ART) provides alternatives for fertility preservation such as oocyte, embryo or ovarian tissue cryopreservation. We reviewed the published literature on fertility-preserving management in breast cancer, aiming at finding evidence to answer the following questions: (1) What are the fertility sparing options available?; (2) How do these women respond to IVF? and (3) Can pregnancy influence breast cancer recurrence? There is a paucity of publications describing clinical experience and outcome data which limits accessibility to fertility preservation in this setting...
June 22, 2018: JBRA Assisted Reproduction
Wei Zhang, Meimei Wang, Shuang Wang, Hongchu Bao, Qinglan Qu, Ning Zhang, Cuifang Hao
OBJECTIVE: To compare the clinical outcomes of follicular versus luteal phase ovarian stimulation in women with poor ovarian response (Bologna criteria) undergoing IVF. METHODS: This retrospective study investigated 446 patients submitted to 507 cycles in three groups. First, the two larger cohorts were examined: 154 patients treated with luteal phase ovarian stimulation (Group Lu); and 231 patients administered follicular phase ovarian stimulation (Group Fo). Then the clinical outcomes of 61 patients submitted to double ovarian stimulation were analyzed...
June 22, 2018: JBRA Assisted Reproduction
Emma Adolfsson, Anna Nowosad Andershed
OBJECTIVE: Our primary aim was to compare the morphology and morphokinetics on inter- and intra-observer agreement for blastocyst with known implantation outcome. Our secondary aim was to validate the morphokinetic parameters' ability to predict pregnancy using a previous published selection algorithm, and to compare this to standard morphology assessments. METHODS: Two embryologists made independent blinded annotations on two occasions using time-lapse images and morphology evaluations using the Gardner Schoolcraft criteria of 99 blastocysts with known implantation outcome...
June 18, 2018: JBRA Assisted Reproduction
Monise Santos, Ivan Henrique Yoshida, Caroline Zulim, Michelli Suemi Tanada, Emerson Barchi Cordts, Caio Parente Barbosa
This paper reports the case of a patient who sought assisted reproductive technology (ART) treatment and was referred to pre-implantation genetic diagnosis (PGD) on account of a chromosomal translocation presented with secondary infertility. The patient underwent a highly complex ART treatment and had 14 metaphase II oocytes collected on the day of follicular aspiration. The embryos were taken to extended culture and five were biopsied and vitrified. The embryo genetic report showed aneuploidy in four of the blastocysts, while the other resulted in 46, XX...
June 18, 2018: JBRA Assisted Reproduction
Matheus Roque, Marcello Valle, Marcos Sampaio, Selmo Geber
OBJECTIVE: To evaluate if there are differences in the risks of obstetric outcomes in IVF/ICSI singleton pregnancies when compared fresh to frozen-thawed embryo transfers (FET). METHODS: This was a systematic review and meta-analysis evaluating the obstetric outcomes in singleton pregnancies after FET and fresh embryo transfer. The outcomes included in this study were pregnancy-induced hypertension (PIH), pre-eclampsia, placenta previa, and placenta accreta. RESULTS: The search yielded 654 papers, 6 of which met the inclusion criteria and reported on obstetric outcomes...
May 21, 2018: JBRA Assisted Reproduction
Ibukun P Oyeyipo, Damilare H Adeyemi, Temilade R Abe
OBJECTIVES: This study aimed to determine the effect of intrauterine exposure to nicotine in the first fourteen days of gestation on the testicular function of male Wistar rats. METHODS: Pups of both control and nicotine-treated groups were selected and sacrificed on day 60 after birth. Birth weight, weight of reproductive organs, hormonal profile, and histopathology were determined in the first filial (F1) generation. RESULTS: Significant decreases in birth weight and litter size were found in the pups treated with nicotine when compared with the animals in the control group...
May 21, 2018: JBRA Assisted Reproduction
Saghar Salehpour, Sedighe Hosseini, Leila Nazari, Nasrin Saharkhiz, Shahrzad Zademodarres
INTRODUCTION: Polycystic ovary syndrome is one of the most common endocrinopathies in young women, and it affects 6% to 8% of women in reproductive age. Hyperandrogenism is the hallmark of polycystic ovary syndrome. The aim of the present study was to evaluate the effects of orlistat on weight loss and serum androgen levels among Iranian women with polycystic ovary syndrome. METHODS: The present study was carried out in the clinic of Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran...
May 14, 2018: JBRA Assisted Reproduction
Larissa Benvenutti, Rafael Alonso Salvador, David Til, Alfred Paul Senn, David Rivero Tames, Nicole Louise Lângaro Amaral, Vera Lúcia Lângaro Amaral
OBJECTIVE: To evaluate the efficiency of two vitrification protocols for rat immature testicular tissue and heterotopic transplantation. METHODS: Twenty-four pre-pubertal Wistar rats were divided into three groups (n=8). After orchiectomy, testicular fragments (3mm) from Groups 1 and 2 were vitrified with different cryoprotectant concentration solutions, using sterile inoculation loops as support. After warming up, the fragments were submitted to cell viability assessment by Trypan blue and histological evaluation...
April 25, 2018: JBRA Assisted Reproduction
Maria do Carmo Borges de Souza, Roberto de Azevedo Antunes, Ana Cristina Allemand Mancebo
No abstract text is available yet for this article.
June 1, 2018: JBRA Assisted Reproduction
Fernanda G E Raffo, Jorge Blaquier
OBJECTIVE: This report presents a summary of the outcomes achieved at ART center FERTILAB in Buenos Aires, Argentina, with transport IVF-ICSI for 25 years (1990-2014). METHODS: This report included all patients submitted to oocyte retrieval for IVF-ICSI whose oocytes were transported from sites of aspiration located 0.5-58 kilometers away from the central laboratory. The numeric data herein reported were taken from annual reports submitted by our institution (Fertilab) and, for purposes of comparison, by all Argentinian centers to the Latin American Registry of Assisted Reproduction (RLA) within the same time period...
June 1, 2018: JBRA Assisted Reproduction
Janisse Ferreri, Emma Gabriela Portillo, Joana Peñarrubia, Ester Vidal, Francisco Fábregues
OBJECTIVE: Pregnancy after an embryo transfer depends largely on embryo quality, endometrial receptivity, and the technique used in the embryo transfer. Embryo transfers have been reported as inevitably traumatic and difficult for 5-7% of patients in assisted reproduction treatment. In these cases, transmyometrial embryo transfer should be considered as a suitable method to overcome difficult embryo transfers. The aim of this study was to report our experience with this technique and analyze its causes, results and complications...
June 1, 2018: JBRA Assisted Reproduction
Rosina Ordoqui, Natalibeth Barrera, José María Montes, Mariel Canepa, Carla Bonelli, Carolina Surka, Andrea Torrens, Lidia Cantú, Stefan S du Plessis
OBJECTIVE: The purpose of this study was to investigate the status of homologous sperm banking in Uruguay. METHODS: A retrospective investigation was performed on data collected between 2013 and 2015. Reasons for sperm banking, patient age, pre-freeze and post-thaw semen parameters, and recovery rates were analyzed. RESULTS: 623 samples were cryobanked between 2013 and 2015. Only 324 samples were considered for analysis after selection based on inclusion criteria...
June 1, 2018: JBRA Assisted Reproduction
Ivan H Yoshida, Monise Santos, Caroline Z Berton, Caroline L Chiarella, Michelli S Tanada, Emerson BCordts, Waldemar P de Carvalho, Caio P Barbosa
OBJECTIVE: Euploid embryo transfers yield better implantation rates. In Brazil, morphological evaluation is performed to select the best embryos, since genetic analysis is still an expensive procedure. This study aimed to evaluate whether there is an association between trophectoderm morphology and ploidy status. METHODS: The study included 113 blastocysts formed in D5/D6 from 58 in vitro fertilization cycles held from January/2016 to May/2017. All patients with indication for PGD/PGS were included in the study...
June 1, 2018: JBRA Assisted Reproduction
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