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JBRA Assisted Reproduction

Joseph Babatunde Dare, Babajide Arogundade, Olakunle Oladipupo Awoniyi, Adebiyi Aderinola Adegoke, Damilare Adedayo Adekomi
OBJECTIVE: Dexamethasone is a widely used glucocorticoid, which has been prescribed increasingly in recent years. The effects of Dexamethasone on the ovary and uterus was investigated in present study. METHODS: Twenty (20) adult female Wistar rats, weighing 130-170 g were assigned to four (4) groups of five (5) animals each. The rats in the control group received saline, while the rats in the experimental group was subjected to oral treatment of dexamethasone of 12 mg/kg, 10 mg/kg, and 7 mg/kg doses daily for a period of 10 days, respectively...
September 3, 2018: JBRA Assisted Reproduction
(no author information available yet)
No abstract text is available yet for this article.
September 1, 2018: JBRA Assisted Reproduction
Layasadat Khorsandi, Ali Akbar Oroojan
OBJECTIVE: To evaluate the hydroethanolic extract toxicity, obtained from Tropaeolum majus L. (TM) on mouse testicular tissue. METHOD: In this experimental study, we used 32 male NMRI mice. The experimental groups received 75, 375 and 750 mg/kg of TM extract, respectively. Twenty-four hours after the last experimental day, serum samples were collected for hormonal measurement. Then, the cauda of epididymis and testis were removed for sperm count and histopathological assessments...
September 1, 2018: JBRA Assisted Reproduction
Olga Triantafyllidou, George Sigalos, Irida Oikonomou, Nikos Vlahos
Granulosa cell tumors (GCTs) account for less than 5% of all ovarian malignancies, occur in younger ages, are usually diagnosed in their early stages, and have a good prognosis. GCTs usually present with features of hyperestrogenism. This paper reports the unusual case of an adult with a GCT with manifestations including amenorrhea, mild hirsutism, infertility, clomiphene citrate (CC) resistance (CC), mildly elevated testosterone, high anti-Müllerian hormone (AMH) and normal estrogen levels. The patient was initially diagnosed with polycystic ovarian syndrome (PCOS), and after four attempts at ovarian stimulation she was diagnosed with CC resistance...
August 24, 2018: JBRA Assisted Reproduction
Natalí S Riva, Claudio Ruhlmann, Rocío S Iaizzo, Carla A Marcial López, Alejandro Gustavo Martínez
OBJECTIVE: Cryopreservation of human spermatozoa is fundamental in assisted reproductive technology. At present, slow freezing techniques are widely used for sperm cryopreservation. Recently, sperm vitrification has been proposed as an alternative to slow freezing. This study aimed to compare the efficiency of slow versus ultra-rapid freezing after thawing and to determine the level of DNA fragmentation in post-thaw normal human semen samples processed through each of the cryopreservation techniques...
August 22, 2018: JBRA Assisted Reproduction
Juan-Enrique Schwarze, Juan Pablo Ceroni, Carolina Ortega-Hrepich, Sonia Villa, Javier Crosby, Ricardo Pommer
The effects of acupuncture on IVF outcomes is still unknown. We carried out a systematic review and meta-analysis of RCT to determine whether acupuncture performed at the time of ET improves outcomes. We searched Medline and Embase from January 1990 to June 2017, for the following terms): (acupuncture; acupuncture therapy) and (reproductive techniques, assisted; in vitro fertilization; embryo transfer). We selected RCT that compared acupuncture with sham acupuncture or no treatment. We included only trials in which acupuncture involved the insertion of needles into traditional meridian points...
August 22, 2018: JBRA Assisted Reproduction
Juan Enrique Schwarze, Johana Canales, Javier Crosby, Carolina Ortega-Hrepich, Sonia Villa, Ricardo Pommer
The aim of this review is to determine if the use of DHEA increases the likelihood of success in patients with POR. We searched MEDLINE and EMBASE using the terms "DHEA and diminished ovarian reserve", "DHEA and poor response", "DHEA and premature ovarian aging". A fixed effects model was used and Peto's method to get the odds ratio (OR) with 95% confidence intervals (CI 95%). For quantitative variables, Cohen's method was used to present the standardized mean differences (SMD) with their corresponding confidence intervals...
August 20, 2018: JBRA Assisted Reproduction
Camila Nogueira, Ivan Henrique Yoshida, Fabia Lima Vilarino, Waldemar Pereira de Carvalho, Emerson Barch Cordts, Caio Parente Barbosa
OBJECTIVE: To evaluate the behavior of seminal parameters over the years - 2010 to May 2017. METHODS : A retrospective study, carried out from January 2010 to May 2017, covering men who underwent sperm examination. Seminal parameters (volume, sperm concentration, motility, morphology, age and duration of infertility in years) of 23,504 men were evaluated. The groups were compared to each other to check whether there were changes to the seminal parameters in that period...
August 17, 2018: JBRA Assisted Reproduction
Sunday A Adelakun, Victor O Ukwenya, Babatunde S Ogunlade, Julius A, Ayooluwa G
OBJECTIVE: To determine the impact of walnut oil on nitrite-induced testicular toxicity in Sprague-Dawley (SD) rats. Available evidence suggests that walnut oil contains high levels of important unsaturated fatty acids including alpha-linolenic acid (ALA) and omega-3; nitrite is a reproductive toxicant that causes the loss of germ cells in the seminiferous tubules and generates oxidative stress in the testes, thus reducing sperm counts and affecting sperm morphology. METHODS: This study included 24 male and 24 female adult SD rats...
August 14, 2018: JBRA Assisted Reproduction
Roberto Coco
The possibility of sequencing hundreds of genes simultaneously and performing molecular karyotyping thanks to the introduction of novel genetic tools has expanded the use of preconception screening for blastocyst recessive mutations and aneuploidies before embryo transfer, with the ultimate purpose of increasing the proportion of normal healthy newborns. Since medically-assisted reproduction procedures are increasingly required to be eugenic, and the aforementioned genetic tests cover only half of the potential genetic diseases occurring at birth, it seems reasonable to incorporate genetic counseling in the practice of assisted reproduction to avoid prosecution for malpractice...
August 14, 2018: JBRA Assisted Reproduction
Claudia G Petersen, Ana L Mauri, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Mariana Mattila, Vanessa Comar, Juliana Ricci, Felipe Dieamant, Joao Batista A Oliveira, Ricardo L R Baruffi, Jose G Franco
OBJECTIVE: This study aimed to evaluate the effects of male age on sperm DNA damage. METHODS: This cross-sectional study included semen samples collected from 2,178 men seen at an infertility clinic. For DNA integrity analysis, the proportions of spermatozoa showing DNA fragmentation (TUNEL assay), abnormal chromatin packaging/underprotamination (chromomycin A3 ), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin V) were recorded...
August 14, 2018: JBRA Assisted Reproduction
Chandra Kailasam, Heather Griffith, Paul Wilson, Uma Gordon
OBJECTIVE: Coasting is a well-known strategy to decrease severity of Ovarian Hyperstimulation Syndrome (OHSS). The purpose of this study is to assess the effect of Coasting on blastocyst development and subsequent clinical outcome following exclusive blastocyst transfer. METHODS: We conducted an observational cohort study of patients having blastocyst transfer following IVF/ICSI treatment. Patients undergoing IVF/ICSI cycles were included in the study. Patients at risk of OHSS were coasted...
August 14, 2018: JBRA Assisted Reproduction
Olugbemi Tope Olaniyan, Olufadekemi Tolulope Kunle-Alabi, Yinusa Raji
OBJECTIVES: Male infertility caused by exposure to heavy metals is a current global issue. Exposure to cadmium chloride (CdCl2 ) negatively affects the male reproductive system. Many infertile people, especially in developing countries, resort to folkloric treatment. Plukenetia conophora is used in Nigerian folk medicine to promote fertility. This study investigated the effects of Plukenetia conophora (PC) and 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-6-Methyl (DDMP) on Wistar rats with cadmium chloride-induced testicular damage...
August 14, 2018: JBRA Assisted Reproduction
Sunday Aderemi Adelakun, Babatunde Ogunlade, Toluwase Solomon Olawuyi, Julius Akomaye Aniah, Olusegun Dare Omotoso
OBJECTIVE: The present study focused on histomorphology, sperm quality, hormonal profile and hematological parameters in adult male Sprague-Dawley rats following the administration of aqueous crude extract of Solanum nigrum by gastric gavage. METHODS: Fourty healthy male adult (12-14 weeks old) Sprague-Dawley rats weighing 200-220g were randomly divided into four groups (A,B,C and D) of ten (n=10) rats each. Group A which served as control were given distilled water 2ml/kg b...
August 1, 2018: JBRA Assisted Reproduction
Sunday Adelakun, Olusegun Omotoso, Julius Aniah, Oyebowale Oyewo
OBJECTIVE: The current study focused on the pro-fertility potential of Senecio biafrae (Sb) extract and vitamin C in Male Sprague Dawley (SD) rats with tetracycline-induced infertility. METHODS: A total of 36 male and 36 female adult SD rats were used for this investigation. The male rats randomly assigned to Group A (controls) were given normal saline 2ml/kg. Rats in Groups B, C, D, E, and F were respectively administered [30 mg/kg of body weight (bwt) of tetracycline], [30 mg/kg bwt of tetracycline + 50 mg/kg of vitamin C], [30 mg/kg bwt of tetracycline + 500 mg/kg bwt of Sb], [30 mg/kg bwt of tetracycline + 50 mg/kg of vitamin C + 500 mg/kg bwt of Sb], and [30 mg/kg bwt of tetracycline reversal] daily for 28 days via gastric gavage...
August 1, 2018: JBRA Assisted Reproduction
(no author information available yet)
No abstract text is available yet for this article.
September 1, 2018: JBRA Assisted Reproduction
Edson Borges, Daniela Paes de Almeida Ferreira Braga, Amanda Souza Setti
No abstract text is available yet for this article.
September 1, 2018: JBRA Assisted Reproduction
Eloísa T Massignam, Maitê Ferreira, Eduardo Sanguinet, Ágata Dupont, Fábio Klamt, Nilo Frantz, Adriana Bos-Mikich
OBJECTIVE: The present study analyzed the quality of bovine ovarian tissue after vitrification in a metal closed chamber, in terms of putative changes in tissue viability (lactate dehydrogenase -LDH- release), anti-oxidant defenses, and redox parameters caused by cryopreservation. METHODS: Small and large fragmented bovine ovarian tissue specimens were vitrified in a metal chamber. After rewarming, tissue samples were fixed or cultured for 48 hours. Glutathione (GSH), protein sulfhydryl content, Total Radical Trapping Antioxidant Potential (TRAP), and lactate dehydrogenase were analyzed immediately after rewarming and after tissue culture...
September 1, 2018: JBRA Assisted Reproduction
Maryam Eftekhar, Ramesh Baradaran Bagheri, Nosrat Neghab, Robabe Hosseinisadat
OBJECTIVE: This study aimed to evaluate the effect of three days of GnRH antagonist pretreatment on the pregnancy outcomes of women with polycystic ovarian syndrome (PCOS) on GnRH antagonist protocols for IVF/ICSI. METHODS: Fifty women with PCOS in the control group received conventional antagonist protocols, starting on day 2 of the cycle. In the pretreatment group (n=38), a GnRH antagonist was administered from day 2 of the menstrual cycle for three days. RESULTS: Controlled ovarian stimulation (COS) duration and gonadotropin dosages were similar in both groups...
September 1, 2018: JBRA Assisted Reproduction
Otoufe Sheikhi, Masoumeh Golsorkhtabaramiri, Sedighe Esmaeilzadeh, Treza Mahouti, Fateme Nadi Heidari
OBJECTIVE: This study set out to investigate the pregnancy outcome of natural cycle regimen versus other endometrial preparation protocols with vitrification thawed blastocyst transfer (VTBT) cycles. METHODS: This control trial study was carried out on 123 women undergoing VTBT. The women were randomly divided into three groups of endometrial preparation before VTBT; 1. Modified natural ovulation cycle with using HCG (n=32) 2. Mild hormonally stimulated cycle by low dose Clomiphene Citrate (n=30) and 3...
September 1, 2018: JBRA Assisted Reproduction
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