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JBRA Assisted Reproduction

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https://www.readbyqxmd.com/read/29338139/occurrence-of-ovarian-follicular-dominance-during-stimulation-for-ivm-impacts-usable-blastocyst-yield
#1
Sergio Romero, Ricardo Pella, Francisco Escudero, Ygor Pérez, Mario García, Patricia Orihuela
OBJECTIVE: To evaluate the influence of ovarian follicular dominance on the outcome of oocyte in-vitro maturation. METHODS: This retrospective cohort study included 21 patients with polycystic ovaries or polycystic ovary syndrome (Rotterdam criteria, 2004) subjected to 24 in-vitro maturation (IVM) cycles between October 2015 and January 2017. Patients undergoing IVM received minimal gonadotropin stimulation starting on day 2 or 3 of the cycle; ovum pick-up typically occurred on days 6 to 8...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29338138/could-cryopreserved-human-semen-samples-be-stored-at-80%C3%A2-c
#2
Carlos R Vaz, Tamara Lamim, Rafael A Salvador, Anna P B Batschauer, Vera Lucia L Amaral, David Til
OBJECTIVE: To evaluate storage time effects in cryopreserved human semen samples, kept in the freezer at a controlled temperature of -80°C, on sperm viability after thawing. METHODS: We used 20 semen samples. Each sample was cryopreserved in 10 fingers, which were divided into five groups: one group was kept in cryogenic canisters throughout the experiment(control), and four groups were kept in a VIP Ultra Low MDF-U76V- PE freezer, with the temperature set at -80°C, for 24, 48, 72 and 96 hours, respectively...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29338137/morphological-embryo-selection-an-elective-single-embryo-transfer-proposal
#3
Francisco Parera Déniz, Carlos Encinas, Jorge La Fuente
OBJECTIVE: To describe a patient selection method for elective single embryo transfer (eSET), emphasizing inclusion criteria and results. METHODS: This retrospective study included all cases seen in a private clinic between June 2011 and December 2016, in La Paz, Bolivia (3600 meters above sea level). Elective single embryo transfer was the method of choice in 34 IVF/ICSI cycles, all in the blastocyst stage. Gardner's blastocyst classification criteria were used...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29338136/plasmatic-estradiol-concentration-in-the-mid-luteal-phase-is-a-good-prognostic-factor-for-clinical-and-ongoing-pregnancies-during-stimulated-cycles-of-in-vitro-fertilization
#4
Rodopiano S Florêncio, Melaynne S B Meira, Marcos V da Cunha, Mylena N C R Camarço, Eduardo C Castro, Marta C C F Finotti, Vinicius A de Oliveira
OBJECTIVE: To evaluate the predictive efficiency of serum estradiol (E2) concentration in the mid-luteal phase regarding chemical, clinical, and ongoing pregnancies, in patients subjected to IVF/ICSI with fresh embryo transfer. METHODS: One hundred and forty-three patients undergoing IVF/ICSI met all the inclusion criteria for the present study. Most of the patients used antagonists, final maturation was achieved with recombinant chorionic gonadotrophin (HCG), and embryo transfer took place on days 3 to 5, but mostly on day 4...
January 16, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29327861/prognostic-factors-in-intrauterine-insemination-cycles
#5
Fernanda Sicchieri, Aline Bomfim Silva, Ana Carolina Japur de Sá Rosa E Silva, Paula Andrea de Albuquerque Sales Navarro, Rui Alberto Ferriani, Rosana Maria Dos Reis
OBJECTIVE: This study aimed to evaluate the clinical pregnancy rate of intrauterine insemination cycles in relation to patient age, cause of infertility, ovulation induction method, number of mature follicles and sperm with progressive motility. METHODS: This retrospective observational study included 237 intrauterine insemination cycles performed from 2011 to 2015 at the Assisted Reproduction Service of the Hospital das Clínicas of the Ribeirão Preto Medical School, University of São Paulo...
January 12, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29318868/embryo-development-until-blastocyst-stage-with-and-without-renewal-of-single-medium-on-day-3
#6
Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales
OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3)...
January 10, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29303236/andro-ivf-a-novel-protocol-for-poor-responders-to-ivf-controlled-ovarian-stimulation
#7
Ludmila Bercaire, Sara Mb Nogueira, Priscila Cm Lima, Vanessa R Alves, Nilka Donadio, Artur Dzik, Mario Cavagna, Renato Fanchin
OBJECTIVE: This study aimed to assess a novel protocol designed to improve poor ovarian response through intra-ovarian androgenization. The endpoints were: number of oocytes and mature oocytes retrieved, fertilization, cancellation and pregnancy rates. METHODS: This prospective crossover study enrolled poor responders from previous ovarian stimulation cycles submitted to a novel protocol called ANDRO-IVF. The protocol included pretreatment with transdermal AndroGel(r) (Besins) 25 mg, oral letrozole 2...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29303234/platelet-rich-plasma-as-an-adjuvant-in-the-endometrial-preparation-of-patients-with-refractory-endometrium
#8
Aixa Molina, Jose Sánchez, William Sánchez, Vanessa Vielma
OBJECTIVE: To improve endometrial quality and implantation rates after the administration of platelet-rich plasma in patients with refractory endometrium. METHODS: 19 patients undergoing in vitro fertilization, aged between 33 and 45 years with a history of refractory endometrium, to whom platelet rich plasma was given by infusion with a catheter into the uterine cavity on the tenth day of the hormone replacement therapy, and then 72 hours after the first administration...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29303233/high-percentages-of-embryos-with-21-18-or-13-trisomy-are-related-to-advanced-paternal-age-in-donor-egg-cycles
#9
Javier García-Ferreyra, Roly Hilario, Julio Dueñas
OBJECTIVE: Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS: A total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos)...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29266898/preimplantation-genetic-diagnosis-for-a-patient-with-multiple-endocrine-neoplasia-type-1-case-report
#10
Aline Dt Lima, Vanessa R Alves, Andressa R Rocha, Ana C Martinhago, Ciro Martinhago, Nilka Donadio, Artur Dzik, Mario Cavagna, Luiz H Gebrim
Preimplantation genetic diagnosis was carried out for embryonic analysis in a patient with multiple endocrine neoplasia type 1 (MEN1). This is a rare autosomal-dominant cancer syndrome and the patients with MEN1 are characterized by the occurrence of tumors in multiple endocrine tissues, associated with germline and somatic inactivating mutations in the MEN1 gene. This case report documents a successful preimplantation genetic diagnosis (PGD) involving a couple at-risk for MEN1 syndrome, with a birth of a healthy infant...
December 21, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29266896/review-on-the-role-of-glutathione-on-oxidative-stress-and-infertility
#11
Oyewopo Adeoye, Johnson Olawumi, Adeleke Opeyemi, Oyewopo Christiania
Infertility is a global health problem and it is one of the most stressful conditions amongst married couples. Even though not lethal, it has been described as a radical life changing problem that carries with it significant psychological trauma. Infertility can be caused by various problems and sometimes it is not possible to establish a cause. Oxidative stress, which arises from an imbalance between reactive oxygen species (ROS) and protective antioxidants, influences the entire reproductive lifespan of men and women...
December 21, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29266893/is-the-risk-of-preeclampsia-higher-in-donor-oocyte-pregnancies-a-systematic-review-and-meta-analysis
#12
Juan Enrique Schwarze, Paula Borda, Pamela Vásquez, Carolina Ortega, Sonia Villa, Javier A Crosby, Ricardo Pommer
OBJECTIVE: Preeclampsia (PE) occurs in 4.6% of pregnancies worldwide. The social phenomenon of increasing maternal age has raised the demand for donor oocytes. Egg donation has allowed women with poor ovarian reserve, premature ovarian failure, genetic disorders or surgical menopause to get pregnant. Recipients provide a unique model of immune response because of the differences in the genetic makeup of mothers and fetuses. In PE, immune tolerance may be impaired as a result of having non-autologous eggs implanted...
December 21, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29257632/vaginismus-in-assisted-reproductive-technology-centers-an-invisible-population-in-need-of-care
#13
Maria do Carmo B de Souza, Marcia C G Gusmão, Roberto A Antunes, Marcelo M de Souza, Ana L S Rito, Paloma Lira, Ana C A Mancebo, Maria A Tamm, Tatiana R Panaino, Maria J Bahia
OBJECTIVE: Genital and sexual pain is still neglected. Consequences may be dramatic, since infertility and sexual dysfunction may be reciprocally linked. This is the first study to focus on the identification of cases of vaginismus in the ART scenario and on the introduction of intra-cycle interventions as part of a comprehensive, integrated and patient-centered perspective. METHODS: This observational prospective study looked into 425 IVF/ICSI cycles and 226 frozen embryo transfers carried out from January 1, 2015 to December 31, 2016, and found seven cases of vaginismus...
December 19, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29124919/location-and-expression-of-juno-in-mice-oocytes-during-maturation
#14
Banri Suzuki, Yukou Sugano, Jun Ito, Haruka Saito, Sueo Niimura, Hideaki Yamashiro
OBJECTIVE: Oocyte-sperm interaction is the essential step in fertilization. Juno, which has been known as Folate receptor 4, is the Izumo1 receptor expressed on the oocyte membrane. This study aims to investigate the location and expression of Juno in mice oocytes during maturation. METHODS: To confirm the stage at which Juno expression begins in the mice oocytes and its location pattern, we performed immunostaining methods. Next, we evaluated Juno mRNA expression by a half quantitative RT-PCR...
November 10, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29120571/a-healthy-hla-matched-baby-born-by-using-a-combination-of-acgh-and-karyomapping-the-first-latin-american-case
#15
Andrea Delgado, Guillermo Llerena, Rosmary Lopez, Jimmy Portella, Naomi Inoue, Luis Noriega-Hoces, Luis Guzman
PGD for HLA typing is a procedure that can be performed when an affected child requires a transplant to treat a non-hereditary disorder related to the hematopoietic and/or immune system. Hematopoietic stem cell transplantation from an HLA-identical donor provides the best treatment option. Three conventional ovarian stimulation procedures for IVF were performed in a couple with a 10-year-old child diagnosed with T-cell acute lymphoblastic leukemia of high risk. Trophectoderm biopsy and aCGH examination were performed on 15 blastocysts, three on the first IVF procedure, four on the second cycle, and eight on the third...
November 9, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29116706/effect-of-sperm-dna-fragmentation-on-embryo-development-clinical-and-biological-aspects
#16
Cristian Alvarez Sedó, Melina Bilinski, Daniela Lorenzi, Heydy Uriondo, Felicitas Noblía, Valeria Longobucco, Estefanía Ventimiglia Lagar, Florencia Nodar
OBJECTIVE: The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers. METHODS: Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41...
November 8, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29105483/fsh-dose-to-stimulate-different-patient-ages-when-less-is-more
#17
Edson Borges, Bianca F Zanetti, Amanda S Setti, Daniela Paf Braga, Rita de Cássia S Figueira, Assumpto Iaconelli
OBJECTIVE: To determine the effect of FSH doses on intracytoplasmic sperm injection (ICSI) outcomes according to the age of the patient. METHODS: Patients undergoing controlled ovarian stimulation (COS) for ICSI cycles in a university-affiliated in vitro fertilization center were split into age groups: ≤35 y.o. (n=1523); >35 and ≤38 y.o. (n=652); >38 and ≤40 y.o. (n=332); and >40 y.o. (n=370). The effect of FSH dose on COS, laboratorial and clinical outomes was determined by linear regression models...
November 6, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29099150/expanded-carrier-screening-in-gamete-donors-of-venezuela
#18
Maria Teresa Urbina, Isaac Benjamin, Randolfo Medina, José Jiménez, Laura Trías, Jorge Lerner
OBJECTIVE: To discuss the implications of expanded genetic carrier screening for preconception purposes based on our practice. METHODS: One hundred and forty-three potential gamete donors aged 20-32 years old (µ=24, 127 females and 16 males), signed informed consent forms and were selected according to the REDLARA guidelines. Blood or saliva samples were examined by one of these genetic carrier screening methods: Genzyme screening for Cystic Fibrosis (CF), Fragile X and Spinal Muscular Atrophy (SMA); Counsyl Universal panel or Recombine Carrier Map...
November 3, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29068182/obesity-and-reproduction-a-study-to-determine-how-effectively-medical-education-enhances-awareness-of-the-reproductive-risks-related-to-obesity
#19
Alice S Rhoton-Vlasak, Kay Roussos-Ross, Girard M Cua, Erica L Odera, Tracy A Irani, Terrie Vasilopoulos
OBJECTIVE: To explore awareness of the reproductive versus the medical risks of obesity in a medical and non-medical college educated population. METHODS: An exploratory prospective research design was used. A 26-question online survey was developed and offered to a sample of medical students/residents (n=325) and non-medical college students (n=102). The data were analyzed using Graph Pad software. RESULTS: 102 non-medical undergraduate students (28% male and 72% female) and 325 resident physicians and medical students (46% male, 47% female, 7% unspecified) responded...
October 25, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29068181/semen-parameters-in-men-with-varicocele-dna-fragmentation-chromatin-packaging-mitochondrial-membrane-potential-and-apoptosis
#20
Felipe Dieamant, Claudia G Petersen, Ana L Mauri, Vanessa Conmar, Mariana Mattila, Laura D Vagnini, Adriana Renzi, Bruna P Costa, Camila Zamara, João Batista A Oliveira, Ricardo L R Baruffi, José G Franco
OBJECTIVE: To evaluate varicocele effects on conventional semen parameters: sperm DNA fragmentation, chromatin packaging, mitochondrial membrane potential (MMP) and sperm apoptosis. METHODS: A cross-sectional study was carried out with semen samples from 2,399 men of couples who attended an infertility clinic. A total of 16.3% (391/2399) of the men were diagnosed with varicocele by a urologist. RESULTS: A regression analysis revealed that the percentages of sperm with DNA fragmentation, abnormal chromatin packaging, and abnormal MMP were significantly increased in individuals with varicocele, when compared to men without varicocele...
October 25, 2017: JBRA Assisted Reproduction
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