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Reports of Biochemistry & Molecular Biology

Munesh Kumar, Rajesh Thakur
Background: Arsenic is a well-documented human carcinogen widely distributed in the environment. Chronic exposure of humans to inorganic arsenicals causes many adverse health effects. The present work was conducted to evaluate the protective effect of Syzygium cumini seed extract (SCE) on arsenic-induced genotoxicity and hepatotoxicity in Wistar albino rats. Methods: Rats were randomly divided into five groups of six animals each. Group 1 served as normal control, Group 2 received SCE, 200 mg/kg daily, and Group 3 received arsenic, 100 ppm in drinking water...
October 2018: Reports of Biochemistry & Molecular Biology
Naghmeh Ahmadiankia, Mehdi Bagheri, Mozhgan Fazli
Background: Triple-negative breast cancer (TNBC) is treated with highly aggressive non-targeted chemotherapies. Safer and more effective therapeutic approaches than those currently in use are needed. Natural pomegranate peel extract (PPE) has recently been found to inhibit breast cancer progression; however, its mechanisms of action remain unclear. We hypothesized that transcriptional changes in the genes encoding the adherence proteins of intercellular adhesion molecule-1 (ICAM-1) and vascular endothelial growth factor (VEGF), may explain, at least in part, the anti-metastatic properties of PPE...
October 2018: Reports of Biochemistry & Molecular Biology
Mehdi Koushki, Nasrin Amiri Dash Atan, Hossein Omidi-Ardali, Mostafa Rezaei Tavirani
Background: Pre-eclampsia (PE) is a pregnancy disorder characterized by hypertension and proteinuria. The evidence has suggested that microRNAs (miRs) are associated with pre-eclampsia pathogenesis; however, these results are inconsistent. The aim of this study was to assess the association between miR-210 expression and PE risk. Methods: Previous studies were selected using PubMed, Scopus, MEDLINE, EMBASE, Science Direct, Google Scholar, Directory of Open Access Journals (DOAJ), and Scientific Information Database (SID)...
October 2018: Reports of Biochemistry & Molecular Biology
Nasiri Sayed Mahdi, Farideh Azarbani, Afshin Pirnia, Abolfazl Abbaszadeh, Mohammadreza Gholami
Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. However, during this process, the stem cells often become damaged which degrades their value for experiments and treatments. Caffeic acid (CA) is an antioxidant that has been shown to increase the viability of cells under stress...
October 2018: Reports of Biochemistry & Molecular Biology
Rajappa Rashmi, Salai Bojan Magesh, Kunka Mohanram Ramkumar, Sarvajayakesavulu Suryanarayanan, Madhunapantula Venkata SubbaRao
Background: Naringenin is a bioactive flavonoid found in grapes and citrus fruits including tangelo, blood orange, lemons, and tangerines. The aims of this study were to investigate the ability of naringenin to scavenge free radicals and determine its ability to protect animals from streptozotocin (STZ) -induced liver damage. Methods: The free radical-scavenging activity of naringenin was evaluated by in vitro cell-free assay systems. In animals, the antioxidant potential of orally administered 50 and 100 mg/kg body weight naringenin for 45 days was assessed by measuring TBARS, lipid hydroperoxides, SOD, catalase, GST, GPx, and glutathione levels in liver homogenates prepared from animals injected intraperitoneally with multiple low dose streptozotocin at 50 mg/kg for five consecutive days...
October 2018: Reports of Biochemistry & Molecular Biology
Touraj Miandoabi, Fariborz Bahrami, Vahideh Moein Vaziri, Soheila Ajdary
Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Leishmania major (L. major) and PpSP42 from Phlebotomus papatasi (Ph. papatasi) . In future studies we will test this construct as a DNA vaccine against zoonotic CL...
October 2018: Reports of Biochemistry & Molecular Biology
Akramsadat Mortazavi, Ebrahim Nematipoor, Mahmoud Djalali, Seyed Ali Keshavarz, Simin Samavat, Mahnaz Zarei, Mohammad Hassan Javanbakht
Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Omega-3 fatty acids have been shown to have both anti-atherogenic and anti-inflammatory effects through inducing the expression and production of adipokines. Adipokines such as apelin, have been observed to play a protective role in the incidence and progression of CVD. The aim of this study was to assess the influence of omega-3 fatty acids supplementation on the serum apelin levels in patients with cardiovascular disease...
October 2018: Reports of Biochemistry & Molecular Biology
Magda Mohamad, Somaia Ebeid, Mohamed Shawky Khater, Mohamad Alsadany
Background: Chronic low-grade inflammation may be a cardinal pathophysiologic feature in the pathogenesis of frailty. Interferon-gamma (INF-γ) is an understudied proinflammatory cytokine in frailty that induces many inflammatory pathways including the guanosine triphosphate cyclohydrolase 1 (GTP-CH1) pathway. Our aim was to evaluate the GTP-CH1 pathway in Egyptian frail elderly subjects. Methods: INF-γ, neopterin, and nitric oxide (NO) levels were measured in 80 participants...
October 2018: Reports of Biochemistry & Molecular Biology
Bahareh Moazen, Amir Zarrinhaghighi, Foroogh Nejatollahi
Background: CD90, a membrane-associated glycoprotein is a marker used to identify mesenchymal stem cells (MSCs). Recent studies have introduced CD90, which induces tumorigenic activity, as a cancer stem cell (CSC) marker in various malignancies. Blocking CD90 activity with anti-CD90 monoclonal antibodies enhanced anti-tumor effects. To date, highly specific antibody single-chain variable fragments (scFvs) have been isolated against various targets and showed promising results in cancer immunotherapy...
October 2018: Reports of Biochemistry & Molecular Biology
Mehrdad Gholami, Alireza Salimi Chirani, Reza Falak, Mona Moshiri, Shabnam Razavi, Gholamreza Irajian
Background: Pseudomonas aeruginosa , an opportunistic pathogen, is a common cause of healthcare-associated infections in immunocompromised individuals. The rapid emergence of multidrug-resistant strains has made P. aeruginosa infections progressively difficult to treat. In this study we evaluated the effect of a chimeric protein containing a P. aeruginosa PilQ fragment and the PilA disulfide loop (PilA-DSL) on the humoral immune response in BALB/c mice. Methods: A chimeric gene encoding an immunogenic region of PilQ and the PilA-DSL was synthesized...
October 2018: Reports of Biochemistry & Molecular Biology
Elnaz Sheikhpour, Parisa Noorbakhsh, Elnaz Foroughi, Soudabeh Farahnak, Rezvan Nasiri, Hossein Neamatzadeh
Interleukin (IL)-10, a multifunctional immune-regulatory cytokine with both immunosuppressive and anti-angiogenic functions, is produced by immune cells including macrophages, T lymphocytes, and natural killer cells. Among other effects, IL-10 promotes tumor cell proliferation and metastasis via immunosuppression. Interleukin-10-mediated immunosuppression is aided by synthesis of tumor necrosis factor, IL-1, IL-12, and chemokines, and down regulation of the surface co-stimulatory molecules CD80 and CD86 on tumors...
October 2018: Reports of Biochemistry & Molecular Biology
Seyed Amir Jalali, Fatemeh Homaei Shandiz, Jalil Tavakol Afshari, Maryam Davarpanah Tanha Ghochan, Amin Reza Nikpoor, Mojgan Mohammadi
Background: The First apoptosis signal (FAS) and First apoptosis signal ligand (FASL) genes initiate the apoptosis pathway, playing a central role in the tumor growth and metastasis. Gene polymorphisms including -1377 G/A in the promoter region of FAS and -844 C/T in the promoter region of FASL have shown to change the transcription activities of these genes. Methods: In this study we evaluated association of these polymorphisms with risk of metastasis of breast cancer, in a population selected from Mashhad, Iran...
October 2018: Reports of Biochemistry & Molecular Biology
Sara Karimkhani, Vahid Chaleshi, Hedieh Balaii, Peyman Tarban, Mahyar Nourian, Shiva Irani, Shabnam Shahrokh, Hamid Asadzadeh Aghdaei, Amir Houshang Mohammad Alizadeh, Mohsen Norouzinia, Mohammad Reza Zali
Background: Inflammatory bowel diseases (IBDs), which include ulcerative colitis (UC) and Crohn's disease (CD), are inflammatory disorders that affect the gastrointestinal tract. A combination of inflammatory cytokines has an important role in IBD development. Genome-wide association studies have shown that polymorphisms in the interleukin-23R gene ( IL-23R ) increase susceptibility to IBD. The aim of this study was to investigate the IL-23R 3' UTR SNP to determine a potential association between genotype distribution and IBD...
October 2018: Reports of Biochemistry & Molecular Biology
Mana Shojapour, Ghasem Mosayebi, Reza Hajihossein, Farshid Noorbakhsh, Aram Mokarizadeh, Mohammad Hossein Ghahremani
Background: The purification of Schwann cells has proven to be a difficult process, with most methods requiring the use of special equipment. However, obtaining a sufficient number and high purity of Schwann cells is an integral aspect in their use for clinical application. Therefore, the aim of this study was to establish a simple and effective protocol for the isolation and purification of Schwann cells from the sciatic nerve of C57BL/6 mice. Furthermore, we aimed to provide a protocol for the isolation of exosomes from these cells...
October 2018: Reports of Biochemistry & Molecular Biology
Abbas Yousefinejad, Fereydoon Siassi, Mohammad Hassan Javanbakht, Hamed Mohammadi, Ehsan Ghaedi, Mahnaz Zarei, Ehsan Djalali, Mahmoud Djalali
Background: Nephrotic syndrome is a disorder caused by kidney damage that results in severe leakage of protein from blood into urine. Hyperlipidemia is one complication of nephrotic syndrome. L-carnitine and genistein can control cardiovascular diseases by causing changes in lipid metabolism and cytokine production. This study was designed to examine the effects of genistein and L-carnitine on serum lipid and cytokine profiles in experimental nephrotic syndrome. Methods: In this study, 50 male Sprague-Dawley rats were randomly divided into five groups of 10 animals each with similar mean body weights (300±50 g)...
October 2018: Reports of Biochemistry & Molecular Biology
Farnaz Sedghy, Abdol-Reza Varasteh, Mojtaba Sankian, Malihe Moghadam
Asthma and allergic diseases cases have risen in recent decades. Plant pollen is considered as the main aeroallergen causing allergic reactions. According to available data, urban residents experience more respiratory allergies than rural residents mainly due to the interaction between chemical air pollutants and pollen grains. This interaction can occur through several mechanisms; chemical pollutants might facilitate pollen allergen release, act as adjuvants to stimulate IgE-mediated responses, modify allergenic potential, and enhance the expression of some allergens in pollen grains...
April 2018: Reports of Biochemistry & Molecular Biology
Tarek Adnan Ahmad, Samar Saeed Rammah, Salah Ahmed Sheweita, Medhat Haroun, Laila Hamdy El-Sayed
Background: Pasteurella multocida continues to pose a danger to prone farm and wild animals all over the world. Chemotherapeutic treatments are progressively losing their effectiveness, last for long time, and cost a lot of money, as well as being toxic to human consumers. Therefore, clearing the way for immunization as a big-wheel alternative against the economic grain. Yet, the vaccines available in the market do not confer the necessary protection against the pathogen. The integration of the well adjuvanted killed vaccine with the attenuated vaccines proved to offer an effective protection to the host animals...
April 2018: Reports of Biochemistry & Molecular Biology
Nazila Ariaee, Jalal Ghorbani, Maryam Panahi, Mojgan Mohamadi, Javad Asili, Alireza Ranjbar, Reza Farid Hoseini, Farahzad Jabbari
Background: Rhinitis, which occurs most commonly as allergic rhinitis and affects 20% of the world's population, is a major health care burden causing significant morbidity. Considering the high prevalence of allergic rhinitis and anti-inflammatory effects of thyme, a favorite condiment, we performed a randomized clinical trial to determine whether thyme can relieve allergic rhinitis symptoms and affect the expression of TH17- and T-regulatory cell- (Treg) related cytokines IL-17, TGF-β, FOXP3, and IL-10...
April 2018: Reports of Biochemistry & Molecular Biology
Ali Aliee, Farnaz Zahedi Avval, Hossein Taheri, Saeedeh Mehraban Moghadam, Mohammad Soukhtanloo, Daryoush Hamidi Alamdari, Bahare Fazeli
Background: Until recently, a gene polymorphism in the promoter region of endothelial nitric oxide synthase has been suggested as a risk factor for thromboangiitis obliterans (TAO) development. The aim of this study was to compare the metabolites of nitric oxide (NO) and its backup, heme-oxygenase-1 (HMOX1), between TAO patients and those of a smoking control group matched by race, age, sex, and smoking habits. Methods: Twenty-four male Caucasian TAO patients and 20 male Caucasian controls enrolled in the study...
April 2018: Reports of Biochemistry & Molecular Biology
Gomaa Mostafa-Hedeab, Dina Sabry, Ghada Mostafa Abdelaziz, Manal Ewaiss, Nagla Adli, Wael Fathy
Background: We explored the effect of vitamin D receptor gene ( VDR ) polymorphisms in response to PEG-IFN treatment in Egyptian chronic hepatitis B (CHB) patients. Methods: Two hundred hepatitis B virus (HBV) patients (42.3±10.7 years) on PEG-IFN α-2a (180 µg /kg for 48 weeks) and one hundred control subjects (37.3 ±12 years) were enrolled in the study. Vitamin D levels and hepatitis B surface antigen (HBsAg) expression were assessed by ELISA. VDR polymorphisms FokI T>C (rs 10735810), BsmI A>G (rs 1544410), ApaI (rs7975253), and TaqI C>T (rs 731236), were genotyped using real-time PCR...
April 2018: Reports of Biochemistry & Molecular Biology
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