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Reports of Biochemistry & Molecular Biology

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https://www.readbyqxmd.com/read/28070537/molecular-analysis-and-expression-of-bap-gene-in-biofilm-forming-multi-drug-resistant-acinetobacter-baumannii
#1
Azizi Omid, Shahcheraghi Fereshteh, Salimizand Himen, Modarresi Farzan, Shakibaie Mohammad Reza, Mansouri Shahla, Ramazanzadeh Rashid, Badmasti Farzad, Nikbin Vajihe
BACKGROUND: Acinetobacter baumannii is commonly resistant to nearly all antibiotics due to presence of antibiotic resistance genes and biofilm formation. In this study we determined the presence of certain antibiotic-resistance genes associated with biofilm production and the influence of low iron concentration on expression of the biofilm-associated protein gene (bap) in development of biofilm among multi-drug-resistant A. baumannii (MDRAB). METHODS: Sixty-five MDRAB isolates from clinical samples were collected...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070536/establishment-a-cho-cell-line-expressing-human-cd52-molecule
#2
Tati Kadijeh, Yazdanpanah-Samani Mahsa, Ramezani Amin, Mahmoudi Maymand Elham, Ghaderi Abbas
BACKGROUND: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells .The aim of this study was to clone and express human CD52 gene in CHO cell line and studying its function in more details...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070535/association-of-fas-a-670g-polymorphism-and-risk-of-uterine-leiomyoma-in-a-southeast-iranian-population
#3
Mohammadpour-Gharehbagh Abbas, Salimi Saeedeh, Keshavarzi Farshid, Zakerian Sepideh, Sajadian Mojtaba, Mokhtari Mojgan
BACKGROUND: Uterine leiomyoma (UL) is a benign tumor of uterine smooth muscle that affects women in reproductive ages. FAS has an important role in initial stages of apoptosis. Previous studies have shown an association between the FAS gene and tumorigenesis. In the present study, we evaluated the relationship between FAS A-670G (rs 1800682) and UL risk. METHODS: The FAS gene polymorphism of 155 women with UL and 157 healthy controls was analyzed by the polymerase chain reaction restriction fragment length polymorphism method...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070534/design-and-construction-of-a-cloning-vector-containing-the-hspx-gene-of-mycobacterium-tuberculosis
#4
Yaghoubi Atieh, Aryan Ehsan, Zare Hosna, Alami Shadi, Teimourpour Roghayeh, Meshkat Zahra
BACKGROUND: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the Mycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX). METHODS: First, an hspX fragment was amplified by PCR and cloned into plasmid pcDNA3...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070533/quantification-of-pla-or-3-a-platanus-orientalis-allergen-grown-under-different-environmental-conditions-by-sandwich-elisa
#5
Sedghy Farnaz, Sankian Mojtaba, Moghadam Maliheh, Varasteh Abdol-Reza
BACKGROUND: Platanus species are widely cultured around the world and considered an important cause of allergic reactions. In the present study, we developed a sandwich ELISA to quantify Pla or 3 allergen in P. orientalis pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control. METHODS: Pollen samples were collected from three polluted and two unpolluted sites in Mashhad, northeast Iran. Recombinant Pla or 3 was expressed and used for polyclonal antibody production in rabbit...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070532/evaluation-of-scd-and-fasn-gene-expression-in-baluchi-iran-black-and-arman-sheep
#6
Salmani Izadi Mohammad, Naserian Abbas Ali, Nasiri Mohammad Reza, Majidzadeh Heravi Reza, Valizadeh Reza
BACKGROUND: With the increasing concern for health and nutrition, dietary fat has attracted considerable attention. The composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. The fatty acid synthase (FASN) and stearoyl-CoA desaturase (delta-9-desaturase) (SCD) genes affect fatty acid composition (1). The expression of SCD and FASN genes is related to an increase in conjugated linoleic acid (CLA) in dairy products, which benefits human health...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070531/are-serum-levels-of-f2-isoprostane-and-oxidized-ldl-related-to-vitamin-d-status-in-type-2-diabetic-patients-a-case-control-study
#7
Javanbakht Mohammad Hassan, Mohammady Hamed, Fooladsaz Koorosh, Razzaghi Maryam, Zarei Mahnaz, Djalali Mahmoud
BACKGROUND: Considerable evidence suggests that oxidative stress affects diabetes mellitus (DM) and contributes to its complications. Vitamin D has been shown to possess antioxidant properties. The aim of this study was to determine the association between serum levels of calcifediol (25-OH-D), an indicator of vitamin D status, and lipid profiles with oxidative stress in patients with type 2 diabetes mellitus (T2DM). METHODS: In this case-control study, 57 T2DM patients with low vitamin D status (< 30 ng/mL) and 48 T2DM patients with normal vitamin D status (> 30 ng/mL) were enrolled...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070530/trace-elements-status-in-sera-of-patients-with-allergic-asthma
#8
Ariaee Nazila, Farid Reza, Shabestari Fahimeh, Shabestari Mohamad, Jabbari Azad Farahzad
BACKGROUND: Asthma is a multifactorial disease and its severity varies with the inflammatory grade. There are conflicting reports about the roles of trace elements in asthma. This study examined the effects of zinc (Zn), copper (Cu), and selenium (Se) concentrations in sera of patients with allergic asthma attending Ghaem Hospital, Mashhad, Iran. METHODS: Forty-nine patients, aged 10 to 50 years, with asthma in moderate or severe stages, and 24 healthy controls, were enrolled in this study...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070529/low-prevalence-of-aeromonas-hydrophilain-infectious-diarrhea-samples-of-pediatric-patients-in-arak-iran
#9
Abbasi Elnaz, Khansari-Nejad Behzad, Abtahi Hamid, Akbari Majid, Ghaznavi-Rad Ehsanollah
BACKGROUND: Aeromonashydrophila (A. hydrophila) is an aquatic bacterium that can cause a spectrum of infectious diseases, including both gastrointestinal and extraintestinal infections. Due to the high rate of diarrheal infections in pediatric patients in central Iran, this study was designed to determine the frequency of A. hydrophila in diarrhea samples from children in this region. METHODS: In this descriptive cross-sectional study, diarrheal stool specimens were collected from 200 children admitted between February and October of 2015 to educational and medical centers affiliated with the Arak University of Medical Sciences...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070528/methylmalonic-acidemia-diagnosis-by-laboratory-methods
#10
Keyfi Fatemeh, Talebi Saeed, Varasteh Abdol-Reza
Methylmalonic acidemia (MMA) is usually caused by a deficiency of the enzyme methylmalonyl-CoA mutase (MCM), a defect in the transport or synthesis of its cofactor, adenosyl-cobalamin (cblA, cblB, cblC, cblF, cblD, and cblX), or deficiency of the enzyme methylmalonyl-CoA epimerase. A comprehensive diagnostic approach involves investigations of metabolites with tandem mass spectrometry, organic acid analysis with gas chromatography, enzymatic studies with fibroblast cell culture, and finally, mutation analysis...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536704/development-and-validation-of-a-gc-fid-method-for-diagnosis-of-methylmalonic-acidemia
#11
Fatemeh Keyfi, Abdolreza Varasteh
BACKGROUND: Urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. In the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. Gas chromatography is the most commonly-used technology to separate and identify these metabolites. In this report the analytical conditions for the determination of methylmalonic acid using a gas chromatography/flame ionization detector (GC-FID) are studied with the aim to establish a method to analyze organic acids in human urine...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536703/sensitivity-comparison-of-the-skin-prick-test-and-serum-and-fecal-radio-allergosorbent-test-rast-in-diagnosis-of-food-allergy-in-children
#12
Hamid Reza Kianifar, Alireza Pourreza, Farahzad Jabbari Azad, Hadis Yousefzadeh, Fatemeh Masomi
BACKGROUND: Diagnosis of food allergy is difficult in children. Food allergies are diagnosed using several methods that include medical histories, clinical examinations, skin prick and serum-specific immunoglobulin E (IgE) tests, radio-allergosorbent test (RAST), food challenge, and supervised elimination diets. In this study we evaluated allergies to cow's milk, egg, peanut, and fish in children with suspected food allergies with skin prick tests and serum and feces RAST. METHODS: Forty-one children with clinical symptoms of food allergies were enrolled in the study...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536702/construction-of-a-novel-dna-vaccine-candidate-encoding-an-hspx-ppe44-esxv-fusion-antigen-of-mycobacterium-tuberculosis
#13
Bagher Moradi, Mojtaba Sankian, Yousef Amini, Zahra Meshkat
BACKGROUND: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. METHODS: A fusion DNA segment consisting of HspX, linker, PPE44, linker, and EsxV, after codon optimization, was designed...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536701/parthenolide-induces-apoptosis-in-committed-progenitor-aml-cell-line-u937-via-reduction-in-osteopontin
#14
Mahdi Zahedpanah, Mojgan Shaiegan, Seyed Hamidollah Ghaffari, Mohsen Nikbakht, Mahin Nikugoftar, Saeed Mohammadi
BACKGROUND: Interfering with cell proliferation and survival is a critical role for antineoplastic drugs leading to cell death through induction of apoptosis. Alternative treatments with herbal extracts offer insights into acute myeloid leukemia (AML) therapy. Parthenolide (PTL), an extract from feverfew, induces apoptosis in primary human leukemia stem cells (LSCs) and bulk leukemic cell populations. Osteopontin (OPN) preserves cell viability in response to anticancer agents and its receptors could be utilized for therapeutic targeting of cancer cells...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536700/comparative-in-silico-study-of-sex-determining-region-y-sry-protein-sequences-involved-in-sex-determining
#15
Masoume Vakili Azghandi, Mohammadreza Nasiri, Ali Shamsa, Mohsen Jalali, Mohammad Mahdi Shariati
BACKGROUND: The SRY gene (SRY) provides instructions for making a transcription factor called the sex-determining region Y protein. The sex-determining region Y protein causes a fetus to develop as a male. In this study, SRY of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences. METHODS: Nucleotide sequences of SRY were retrieved from the NCBI databank...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536699/in-silico-prediction-and-docking-of-tertiary-structure-of-luxi-an-inducer-synthase-of-vibrio-fischeri
#16
Mohammed Zaghlool Saeed Al-Khayyat, Ammar Ghanem Ameen Al-Dabbagh
BACKGROUND: LuxI is a component of the quorum sensing signaling pathway in Vibrio fischeriresponsible for the inducer synthesis that is essential for bioluminescence. METHODS: Homology modeling of LuxI was carried out using Phyre2 and refined with the GalaxyWEB server. Five models were generated and evaluated by ERRAT, ANOLEA, QMEAN6, and Procheck. RESULTS: Five refined models were generated by the GalaxyWEB server, with Model 4 having the greatest quality based on the QMEAN6 score of 0...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27536698/ricin-toxicity-clinical-and-molecular-aspects
#17
Mohammad Moshiri, Fatemeh Hamid, Leila Etemad
Seeds of the castor bean plant Ricinuscommunis L (CB) contain ricin toxin (RT), one of the most poisonous naturally-occurring substances known. Ricin toxin, a water-soluble glycoprotein that does not partition into the oil extract, is a ribosome-inactivating toxin composed of two chains, labeled A and B. Severity of the toxicity varies depending on the route of exposure to the toxin. Inhalational is the most toxic route, followed by oral ingestion. Orally-ingested RT accumulates in the liver and spleen but other cells are also affected...
April 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/26989750/in-silico-analysis-and-modeling-of-acp-mip-pilq-chimeric-antigen-from-neisseria-meningitidis-serogroup-b
#18
Mehrdad Gholami, Alireza Salimi Chirani, Mona Moshiri, Mansour Sedighi, Abazar Pournajaf, Masoud Tohidfar, Gholamreza Irajian
BACKGROUND: Neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5-15% of adults. The aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type IV pilus biogenesis protein antigen (ACP-MIP-PilQ) from N. meningitidis serogroup B strain. METHODS: Biochemical properties and multiple alignments were predicted by appropriate web servers...
October 2015: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/26989749/association-of-htra1-gene-polymorphisms-with-the-risk-of-developing-amd-in-iranian-population
#19
Mohammad Askari, Amin Reza Nikpoor, Fazel Gorjipour, Mohsen Mazidi, Mohammad Hosein Sanati, Hajar Aryan, Alireza Irani, Khalil Ghasemi Falavarjani, Hossein Nazari, Kazem Mousavizadeh
BACKGROUND: Half of the cases of vision loss in people under 60 years of age have been attributed to age-related macular degeneration (AMD). This is a multifactorial disease with late onset. It has been demonstrated that many different genetic loci are implicated in the risk of developing AMD in different populations. In the current study, we investigated the association of high-temperature ‎requirement A-1 (HTRA1) gene polymorphisms with the risk of developing AMD in the Iranian population...
October 2015: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/26989748/in-silico-analysis-and-molecular-modeling-of-rna-polymerase-sigma-s-rpos-protein-in-pseudomonas-aeruginosa-pao1
#20
Mansour Sedighi, Mohsen Moghoofei, Ebrahim Kouhsari, Abazar Pournajaf, Behzad Emadi, Masoud Tohidfar, Mehrdad Gholami
BACKGROUND: Sigma factors are proteins that regulate transcription in bacteria. Sigma factors can be activated in response to different environmental conditions. The rpoS (RNA polymerase, sigma S) gene encodes sigma-38 (σ38, or RpoS), a 37.8 kDa protein in Pseudomonas aeruginosa (P. aeruginosa) strains. RpoS is a central regulator of the general stress response and operates in both retroactive and proactive manners; not only does it allow the cell to survive environmental challenges; it also prepares the cell for subsequent stresses (cross-protection)...
October 2015: Reports of Biochemistry & Molecular Biology
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