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Reports of Biochemistry & Molecular Biology

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https://www.readbyqxmd.com/read/29766006/interaction-between-air-pollutants-and-pollen-grains-the-role-on-the-rising-trend-in-allergy
#1
Farnaz Sedghy, Abdol-Reza Varasteh, Mojtaba Sankian, Malihe Moghadam
Asthma and allergic diseases cases have risen in recent decades. Plant pollen is considered as the main aeroallergen causing allergic reactions. According to available data, urban residents experience more respiratory allergies than rural residents mainly due to the interaction between chemical air pollutants and pollen grains. This interaction can occur through several mechanisms; chemical pollutants might facilitate pollen allergen release, act as adjuvants to stimulate IgE-mediated responses, modify allergenic potential, and enhance the expression of some allergens in pollen grains...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29766005/the-enhancement-of-the-pasteurella-s-bacterin-by-propolis-extracts
#2
Tarek Adnan Ahmad, Samar Saeed Rammah, Salah Ahmed Sheweita, Medhat Haroun, Laila Hamdy El-Sayed
Background: Pasteurella multocida continues to pose a danger to prone farm and wild animals all over the world. Chemotherapeutic treatments are progressively losing their effectiveness, last for long time, and cost a lot of money, as well as being toxic to human consumers. Therefore, clearing the way for immunization as a big-wheel alternative against the economic grain. Yet, the vaccines available in the market do not confer the necessary protection against the pathogen. The integration of the well adjuvanted killed vaccine with the attenuated vaccines proved to offer an effective protection to the host animals...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29766004/oral-administration-of-zataria-multiflora-extract-decreases-il-17-expression-in-perennial-allergic-rhinitis
#3
Nazila Ariaee, Jalal Ghorbani, Maryam Panahi, Mojgan Mohamadi, Javad Asili, Alireza Ranjbar, Reza Farid Hoseini, Farahzad Jabbari
Background: Rhinitis, which occurs most commonly as allergic rhinitis and affects 20% of the world's population, is a major health care burden causing significant morbidity. Considering the high prevalence of allergic rhinitis and anti-inflammatory effects of thyme, a favorite condiment, we performed a randomized clinical trial to determine whether thyme can relieve allergic rhinitis symptoms and affect the expression of TH17- and T-regulatory cell- (Treg) related cytokines IL-17, TGF-β, FOXP3, and IL-10...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29766003/the-status-of-nitric-oxide-and-its-backup-heme-oxygenase-1-in-thromboangiitis-obliterans
#4
Ali Aliee, Farnaz Zahedi Avval, Hossein Taheri, Saeedeh Mehraban Moghadam, Mohammad Soukhtanloo, Daryoush Hamidi Alamdari, Bahare Fazeli
Background: Until recently, a gene polymorphism in the promoter region of endothelial nitric oxide synthase has been suggested as a risk factor for thromboangiitis obliterans (TAO) development. The aim of this study was to compare the metabolites of nitric oxide (NO) and its backup, heme-oxygenase-1 (HMOX1), between TAO patients and those of a smoking control group matched by race, age, sex, and smoking habits. Methods: Twenty-four male Caucasian TAO patients and 20 male Caucasian controls enrolled in the study...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29766002/influence-of-vitamin-d-receptor-gene-polymorphisms-on-response-to-pegylated-interferon-in-chronic-hepatitis-b-egyptian-patients
#5
Gomaa Mostafa-Hedeab, Dina Sabry, Ghada Mostafa Abdelaziz, Manal Ewaiss, Nagla Adli, Wael Fathy
Background: We explored the effect of vitamin D receptor gene ( VDR ) polymorphisms in response to PEG-IFN treatment in Egyptian chronic hepatitis B (CHB) patients. Methods: Two hundred hepatitis B virus (HBV) patients (42.3±10.7 years) on PEG-IFN α-2a (180 µg /kg for 48 weeks) and one hundred control subjects (37.3 ±12 years) were enrolled in the study. Vitamin D levels and hepatitis B surface antigen (HBsAg) expression were assessed by ELISA. VDR polymorphisms FokI T>C (rs 10735810), BsmI A>G (rs 1544410), ApaI (rs7975253), and TaqI C>T (rs 731236), were genotyped using real-time PCR...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29766001/production-of-recombinant-streptavidin-and-optimization-of-refolding-conditions-for-recovery-of-biological-activity
#6
Raoufe Modanloo Jouybari, Abdorrahim Sadeghi, Behzad Khansarinejad, Shabnam Sadoogh Abbasian, Hamid Abtahi
Background: Streptavidin is a protein produced by Streptomyces avidinii with strong biotin-binding ability. The non-covalent, yet strong bond between these two molecules has made it a preferable option in biological detection systems. Due to its extensive use, considerable attention is focused on streptavidin production by recombinant methods. Methods: In this study, streptavidin was expressed in Escherichia coli (E. coli) BL21 (DE3) pLysS cells and purified by affinity chromatography...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29766000/vitamin-d3-induces-gene-expression-of-ox-ldl-scavenger-receptors-in-streptozotocin-induced-diabetic-rat-aortas-new-insight-into-the-role-of-vitamin-d-in-diabetic-atherosclerosis
#7
Shahab Alizadeh, Abbas Mirshafiey, Mahmoud Djalali, Ehsan Alvandi, Niyaz Mohammadzadeh Honarvar, Mohammad Hassan Javanbakht
Background: Several lines of evidence suggest that oxidized LDL (Ox-LDL) scavenger receptors play a crucial role in the genesis and progression of diabetic atherosclerosis. This study aimed to elucidate the effect of vitamin D3 on gene expression of lectin-like oxidized LDL receptor-1 (LOX-1), scavenger receptor-A (SR-A), Cluster of Differentiation 36 (CD36), and Cluster of Differentiation 68 (CD68) as the main Ox-LDL receptors in streptozotocin (STZ)-induced diabetic rat aortas. Methods: Eighteen Sprague-Dawley rats were randomly divided into three groups of six rats each...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765999/genetic-variability-of-antigen-b8-1-among-echinococcus-granulosus-isolates-from-human-cattle-and-sheep-in-fars-province-southern-iran
#8
Asieh Fatemi Esfedan, Bahador Sarkari, Fataneh Mikaeili
Background: Cystic echinococcosis (CE), known as hydatid cyst, is a zoonotic parasitic infection caused by the larval stage of Echinococcus granulosus (E. granulosus) . Antigen B, the major component of hydatid cyst fluid, is encoded by members of a multigene family. The present study aimed to evaluate the genetic diversity of the gene encoding antigen B8/1 ( EgAgB8/1 ) among the main intermediate hosts of E. granulosus . Methods: Twenty-eight hydatid cyst isolates (10 sheep, 9 human, and 9 cattle) were collected in Fars province, Iran...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765998/cloning-expression-purification-and-cd-analysis-of-recombinant-human-betatrophin
#9
Samaneh Gholami, Nematolah Gheibi, Reza Falak, Koorosh Goodarzvand Chegini
Background: Betatrophin is a member of the angiopoietin-like (ANGPTL) family that has been implicated in both triglyceride and glucose metabolism. The physiological functions and molecular targets of this protein remain largely unknown; hence, a purified available protein would aid study of the exact role of betatrophin in lipid or glucose metabolism. Methods: In this study, we cloned the full-length cDNA of betatrophin from a human liver cDNA library. Betatrophin was expressed in the pET-21b- E...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765997/effects-of-oral-probiotic-feeding-on-toll-like-receptor-gene-expression-of-the-chicken-s-cecal-tonsil
#10
Fatemeh Asgari, Reza Falak, Shahram Teimourian, Babak Pourakbari, Salimeh Ebrahimnezhad, Mehdi Shekarabi
Background: It was proposed that probiotics may influence immune system through direct or indirect exposure. Direct exposure is mostly mediated by surface receptors. Toll-like receptors (TLRs) are conserved molecular sensors which could be triggered via some pathogen associated structures, hence, modulate the immune responses. This study was conducted to elucidate the impact of lactobacillus acidophilus as a common probiotic on the expression level of TLRs in the chicken's cecal tonsil...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765996/an-in-vitro-study-on-curcumin-delivery-by-nano-micelles-for-esophageal-squamous-cell-carcinoma-kyse-30
#11
Sare Hosseini, Jamshidkhan Chamani, Hamidreza Rahimi, Navid Azmoodeh, Faezeh Ghasemi, Porya Hassan Abadi
Background: The incidence of esophageal squamous cell carcinoma (ESCC) is increasing, causing catastrophic health burdens on communities. Curcumin has shown promise as a therapeutic agent in the treatment of colon, colorectal, pancreatic, and esophageal cancers but it has very poor bioavailability. The application of nano-carriers as drug delivery systems increases curcumin's bioavailability. Cyclin D1 is overexpressed in ESCC and curcumin may change its expression. Methods: In this study, the effect of SinaCurcumin®, a novel nano-micelle product containing 80 mg curcumin, on the growth of KYSE-30 cells and expression of cyclin D1, was investigated...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765995/effects-of-oral-dosage-of-lead-acetate-ii-on-osteocalcin-gene-expression-in-rat-mesenchymal-stem-cells
#12
Hossein Rafiei, Mehrdad Shariati, Jina Khayatzadeh, Sima Afsharnezhad, Majid Farhoodi
Background: Lead (Pb) is a heavy metal that has devastating effects on many animal tissues. In this study we investigated the effects of orally-dosed lead acetate II on osteocalcin gene ( osteocalcin ) expression in mesenchymal stem cells grown in an osteogenic medium. Osteocalcin is an abundant bone matrix differentiation protein. Methods: Twelve male Wistar rats were divided into three groups of four rats each. Two groups were fed orally with 50 or 100 ppm of lead acetate II with libitum feed and water for two months...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765994/heterologous-expression-purification-and-characterization-of-the-hspx-ppe44-and-esxv-proteins-of-mycobacterium-tuberculosis
#13
Yousef Amini, Mohsen Tafaghodi, Saeid Amel Jamehdar, Zahra Meshkat, Bagher Moradi, Mojtaba Sankian
Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis , including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes were linked together by AEAAAKEAAAKA linkers and inserted into pET21b and pET32b vectors...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29765993/investigating-pik-3-r-3-and-atp-2-a-1-genes-expressions-in-ventilator-associated-pneumonia-patients-admitted-to-the-intensive-care-unit-of-masih-daneshvari-hospital-in-2016
#14
Hamidreza Jamaati, Naghmeh Bahrami, Mahya Daustany, Payam Tabarsi, Behrooz Farzanegan, Seyed Mohammadreza Hashemian, Abdolreza Mohamadnia
Background: Infectious diseases such as ventilator- associated pneumonia (VAP) are one of the serious problems in intensive care units (ICU) of hospitals. To date, there has been no appropriate clinical and diagnostic marker for early detection of this disease. In this study, expression of PIK3 R3 and ATp2 A1 genes in patients with VAP were assessed to be used as biomarkers to identify and confirm the disease. Methods: This study was conducted by using peripheral blood samples of 60 individuals, including 30 patients with VAP and 30 healthy volunteers...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29761109/mesenchymal-stem-cells-differentiate-to-endothelial-cells-using-recombinant-vascular-endothelial-growth-factor-a
#15
Mohsen Khaki, Ali Hatef Salmanian, Hamid Abtahi, Ali Ganji, Ghasem Mosayebi
Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21 pLysS competent cells with the pET32a expression vector. Recombinant VEGF-A protein expression was verified by SDS-PAGE and western blotting...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29090237/lack-of-an-association-between-a-functional-polymorphism-in-the-mdm2-promoter-and-breast-cancer-in-women-in-northeast-iran
#16
Zeinab Tavakkol Afshari, Amin Reza Nikpoor, Jalil Tavakkol Afshari, Rashin Ganjali, Parvaneh Sanglakh Ghoochan Atigh, Fatemeh Homaei Shandiz, Khadijeh Jamialahmadi
BACKGROUND: Breast cancer is one of the most common cancers among women worldwide. Tumor protein 53 (TP53) and its regulator, the mouse double murine 2 (MDM2) protein homologue, influence tumorigenesis through their key roles in cell division and response to DNA damage. The MDM2 SNP309T>G (rs2279744) polymorphism in the promoter region of the MDM2 can cause dysfunction and inactivation of TP53, which promotes tumor progression. The aim of this study was to investigate the possible association between this polymorphism and breast cancer in a northeastern Iranian population...
October 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29090236/expression-of-th1-and-th2-cytokine-and-associated-transcription-factors-in-peripheral-blood-mononuclear-cells-and-correlation-with-disease-severity
#17
Hadis Yousefzadeh, Farahzad Jabbari Azad, Maryam Rastin, Mahnaz Banihashemi, Mahmoud Mahmoudi
BACKGROUND: Psoriasis is a T cell-mediated autoimmune disease in patients with elevated levels of proinflammatory cytokines belonging mainly to the Th1 pathway. We investigated whether treatment of psoriasis patients with methotrexate (MTX), along with micronutrients, modulated mRNA expression of Th1 and Th2 components and whether expression of these components correlated with psoriasis severity. METHODS: Thirty plaque-type psoriasis patients with Psoriasis Area and Severity Index (PASI) scores greater than 10 were recruited; these were 15 non-micronutrients taking- (NMT) patients treated with MTX daily (0...
October 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29090235/construction-of-mtb72f-plasmid-as-a-dna-vaccine-candidate-for-mycobacterium-tuberculosis
#18
Maryam Sadat Nabavinia, Mohammad Ramezani, Aida Gholoobi, Mahboubeh Naderinasab, Zahra Meshkat
BACKGROUND: With one-third of the world's population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should stimulate cellular immunity. DNA vaccines are a new generation of vaccines with the potential to achieve this goal...
October 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29090234/altered-expression-of-cell-cycle-regulators-in-adult-t-cell-leukemia-lymphoma-patients
#19
Reza Torshizi, Ehsan Ghayour Karimani, Kobra Etminani, Mohammad Mehdi Akbarin, Khadijeh Jamialahmadi, Abbas Shirdel, Hossein Rahimi, Abolghasem Allahyari, Amin Golabpour, Houshang Rafatpanah
BACKGROUND: Adult T-cell leukemia/lymphoma (ATLL) is caused by human T-cell lymphotropic virus type-1 (HTLV-1). HTLV-1 oncogenes can induce malignancy through controlled gene expression of cell cycle checkpoints in the host cell. HTLV-I genes play a pivotal role in overriding cell cycle checkpoints and deregulate cellular division. In this study, we aimed to determine and compare the HTLV-1 proviral load and the gene expression levels of cyclin-dependent kinase-2 (CDK2), CDK4, p53, and retinoblastoma (Rb) in ATLL and carrier groups...
October 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29090233/common-variations-in-perilipin-rs1052700-and-fto-rs3751812-gene-variants-and-risk-for-obesity-and-type-2-diabetes
#20
Ramin Saravani, Hamid Reza Galavi, Nafiseh Noorzehi, Nasrin Ranjbar, Fatemeh Mollashahee-Kohkan
BACKGROUND: Perilipins are proteins that coat lipid globules in adipocytes, which are steroid-generating cells that play a central role in lipid storage and breakdown. The FTO gene is associated with type-2 diabetes (T2D) and increased fat mass. In this work the association of perilipin and FTO gene polymorphisms in T2D was investigated. METHODS: One hundred eighty-three Iranian men and women with T2D, and 174 healthy controls with no known metabolic diseases, participated in the study...
October 2017: Reports of Biochemistry & Molecular Biology
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