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Reports of Biochemistry & Molecular Biology

Zeinab Tavakkol Afshari, Amin Reza Nikpoor, Jalil Tavakkol Afshari, Rashin Ganjali, Parvaneh Sanglakh Ghoochan Atigh, Fatemeh Homaei Shandiz, Khadijeh Jamialahmadi
BACKGROUND: Breast cancer is one of the most common cancers among women worldwide. Tumor protein 53 (TP53) and its regulator, the mouse double murine 2 (MDM2) protein homologue, influence tumorigenesis through their key roles in cell division and response to DNA damage. The MDM2 SNP309T>G (rs2279744) polymorphism in the promoter region of the MDM2 can cause dysfunction and inactivation of TP53, which promotes tumor progression. The aim of this study was to investigate the possible association between this polymorphism and breast cancer in a northeastern Iranian population...
October 2017: Reports of Biochemistry & Molecular Biology
Hadis Yousefzadeh, Farahzad Jabbari Azad, Maryam Rastin, Mahnaz Banihashemi, Mahmoud Mahmoudi
BACKGROUND: Psoriasis is a T cell-mediated autoimmune disease in patients with elevated levels of proinflammatory cytokines belonging mainly to the Th1 pathway. We investigated whether treatment of psoriasis patients with methotrexate (MTX), along with micronutrients, modulated mRNA expression of Th1 and Th2 components and whether expression of these components correlated with psoriasis severity. METHODS: Thirty plaque-type psoriasis patients with Psoriasis Area and Severity Index (PASI) scores greater than 10 were recruited; these were 15 non-micronutrients taking- (NMT) patients treated with MTX daily (0...
October 2017: Reports of Biochemistry & Molecular Biology
Maryam Sadat Nabavinia, Mohammad Ramezani, Aida Gholoobi, Mahboubeh Naderinasab, Zahra Meshkat
BACKGROUND: With one-third of the world's population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should stimulate cellular immunity. DNA vaccines are a new generation of vaccines with the potential to achieve this goal...
October 2017: Reports of Biochemistry & Molecular Biology
Reza Torshizi, Ehsan Ghayour Karimani, Kobra Etminani, Mohammad Mehdi Akbarin, Khadijeh Jamialahmadi, Abbas Shirdel, Hossein Rahimi, Abolghasem Allahyari, Amin Golabpour, Houshang Rafatpanah
BACKGROUND: Adult T-cell leukemia/lymphoma (ATLL) is caused by human T-cell lymphotropic virus type-1 (HTLV-1). HTLV-1 oncogenes can induce malignancy through controlled gene expression of cell cycle checkpoints in the host cell. HTLV-I genes play a pivotal role in overriding cell cycle checkpoints and deregulate cellular division. In this study, we aimed to determine and compare the HTLV-1 proviral load and the gene expression levels of cyclin-dependent kinase-2 (CDK2), CDK4, p53, and retinoblastoma (Rb) in ATLL and carrier groups...
October 2017: Reports of Biochemistry & Molecular Biology
Ramin Saravani, Hamid Reza Galavi, Nafiseh Noorzehi, Nasrin Ranjbar, Fatemeh Mollashahee-Kohkan
BACKGROUND: Perilipins are proteins that coat lipid globules in adipocytes, which are steroid-generating cells that play a central role in lipid storage and breakdown. The FTO gene is associated with type-2 diabetes (T2D) and increased fat mass. In this work the association of perilipin and FTO gene polymorphisms in T2D was investigated. METHODS: One hundred eighty-three Iranian men and women with T2D, and 174 healthy controls with no known metabolic diseases, participated in the study...
October 2017: Reports of Biochemistry & Molecular Biology
Mojgan Mohammadi, Mohammad Hossein Gozashti, Majid Aghadavood, Mohammad Reza Mehdizadeh, Mohammad Mahdi Hayatbakhsh
BACKGROUND: Several components of metabolic syndrome (MetS) facilitate its diagnosis, including abdominal obesity, hyperlipidemia, high blood pressure, and insulin resistance. The production of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) seem to be associated with MetS components. The aim of this study was to evaluate the correlation between IL-6 and TNF-α serum levels with MetS and its components. METHODS: This case-control study investigated 250 subjects, comprising 125 healthy controls from the Kerman Blood Transfusion Organization and 125 MetS patients...
October 2017: Reports of Biochemistry & Molecular Biology
Fatemeh Sadat Toghraie, Seyedeh Masoumeh Sharifzadeh, Amin Ramezani, Elham Mahmoudi Maymand, Mahsa Yazdanpanah-Samani, Abbas Ghaderi
BACKGROUND: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. METHODS: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-h IL -7 recombinant by electroporation...
October 2017: Reports of Biochemistry & Molecular Biology
Azadeh Shojaei, Reza Ebrahimzadeh-Vesal, Ali Ahani, Maryam Razzaghy-Azar, Golnaz Khakpour, Farideh Ghazi, Javad Tavakkoly-Bazzaz
BACKGROUND: Disorders of sex development (DSDs) belong to uncommon pathologies and result from abnormalities during gonadal determination and differentiation. Various gene mutations involved in gonadal determination and differentiation have been associated with gonadal dysgenesis. Despite advances in exploration of genes and mechanisms involved in sex disorders, most children with severe 46,XY DSDs have no definitive etiological diagnoses; therefore, the possibility that other genes or loci might play important roles in these disorders needs to be explored...
October 2017: Reports of Biochemistry & Molecular Biology
Samaneh Sepahi, Alireza Pasdar, Sina Gerayli, Sina Rostami, Aida Gholoobi, Zahra Meshkat
BACKGROUND: The prevalence of hepatitis C virus (HCV) infection is increasing worldwide. Cytotoxic Tlymphocyte-associated protein 4 ( CTLA-4 ) may play a role in the intensity of the disease. The aim of this study was to evaluate the association between genetic variants of the CTLA-4 and HCV infection. METHODS: Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was performed as the genotyping assay at four different positions (+49 A>G, -318 C>T, -1722 T>C, and - 1661 A>G)...
October 2017: Reports of Biochemistry & Molecular Biology
Fatemeh Keyfi, Zoltan Lukacs, Abdolreza Varasteh
BACKGROUND: Organic acids refer to a family of compounds that are intermediates in a variety of metabolic pathways. Many organic acids are present in urine from clinically normal individuals. Elevated levels of urine organic acids cause to the organic acidurias, disorders in which some metabolic pathways in organic acid metabolism are blocked. The present work identified major and minor urinary acidic metabolites in normal subjects, and their quantitative ranges in a pediatric population of Iran...
October 2017: Reports of Biochemistry & Molecular Biology
Kabbathi Raghunathachar Sahana, Prashant Akila, Vishwanath Prashant, Bellekere Sharath Chandra, Maduvanahalli Nataraj Suma
BACKGROUND: Determination of the impact of angiogenesis on tumor development and progression is essential. This study aimed to determine the serum levels of Vascular endothelial growth factor (VEGF) and Interleukin 6 (IL-6) in breast carcinoma, and to correlate them with tumor size, lymph node involvement, and cancer stage. METHODS: Under aseptic precautions 5 ml of venous blood was collected from 37 breast cancer patients and 20 healthy females after obtaining due consent and ethical committee clearance...
October 2017: Reports of Biochemistry & Molecular Biology
Chikkavadaragudi Ramachandra Shobha, Vishwanath Prashant, Prashant Akila, Rangaswamy Chandini, Maduvanahalli Nataraj Suma, Hattur Basavanagowdappa
BACKGROUND: Natural products have gained importance recently for the treatment of obesity and its complications, partly because of the side effects of modern drugs.Hence, we aimed to study and compare the effect of varying concentrations of Momordicacharantiaon adipogenesis and adipolysis using 3T3-L1 pre-adipocyte cell lines. METHODS: 3T3-L1 pre-adipocytes were procured from the National Center for Cell Sciences, Pune, and cultured in Dulbecco's Modified Eagle's Media (DMEM) supplemented with 20% fetal bovine serum (FBS) and 1 mM L-glutamine...
October 2017: Reports of Biochemistry & Molecular Biology
Farzad Khademi, Arshid Yousefi-Avarvand, Mohammad Derakhshan, Zahra Meshkat, Mohsen Tafaghodi, Kiarash Ghazvini, Ehsan Aryan, Mojtaba Sankian
BACKGROUND: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. METHODS: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested with the same enzymes and the construct was ligated into pET-21b (+). The accuracy of cloning was confirmed by colony PCR and sequencing...
October 2017: Reports of Biochemistry & Molecular Biology
Farideh Hosseinzadeh, Saeed Mohammadi, Foroogh Nejatollahi
BACKGROUND: Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) molecules are expressed on T-cells and inhibit their function by inhibiting activation of subsequent T-cell molecular pathways. Blocking of CTLA-4 inhibits the growth of malignant tumor cells. Anti-CTLA-4 monoclonal antibodies activate the immune system against cancer. Due to several advantages of single-chain antibodies (scFvs) compared to monoclonal antibodies in cancer immunotherapy, specific anti-CTLA-4 scFvs (single-chain variable fragment) were selected in this study...
October 2017: Reports of Biochemistry & Molecular Biology
Mostafa Cheraghi, Gholamreza Shahsavari, Ali Maleki, Hassan Ahmadvand
BACKGROUND: Dyslipidemia is considered an independent risk factor for coronary heart disease (CHD). In the present study, we examined lipid profiles and paraoxonase 1 (PON1) activity and atherogenic indexes status and the relationship of PON1 activity by high-density lipoprotein (HDL) and atherogenic indexes in CHD patients and healthy people. METHODS: The aim of the study was to compare PON1, lipid profiles, and atherogenic indexes in CHD patients and healthy people as controls...
October 2017: Reports of Biochemistry & Molecular Biology
Mahmoud Haghighat, Mozhgan Moghtaderi, Shirin Farjadian
BACKGROUND: Familial Mediterranean fever (FMF) is an autosomal recessive genetic disorder characterized by recurrent episodes of self-limited fever and serosal tissues inflammation. METHODS: To evaluate clinical symptoms and common genetic mutations in southwestern Iranian patients with FMF, 20 unrelated patients were enrolled in this study based on clinical criteria. A panel of 12 common MEFV gene mutations was tested. RESULTS: The most frequent clinical presentations of the patients were fever, colicky abdominal pain and arthritis...
April 2017: Reports of Biochemistry & Molecular Biology
Sofieh Mousavian-Roshanzamir, Ali Makhdoumi-Kakhki
This study was intended to investigate the effectiveness of static magnetic field on the growth of Escherichia coli (E. coli) provided from two sources, the urine samples of patients with urinary tract infections and the reference strain E. coli ATCC 25922. Bacterial samples in Nutrient Broth were subjected to a range of magnetic intensities (2, 4, 6, 9, 14, 16, 18, and 20 mT) at various exposure times (0, 15, 30, 45, 60, 75, and 90 min). The survival rate was measured in the presence and absence of the magnetic field over time...
April 2017: Reports of Biochemistry & Molecular Biology
Zeeba Kamaliyan, Sara Pouriamanesh, Mona Amin-Beidokhti, Amir Rezagholizadeh, Reza Mirfakhraie
BACKGROUND: The PIWI-interacting RNA (piRNA) pathway has an essential role in transposon silencing, meiosis progression, spermatogenesis, and germline maintenance. HIWI genes are critical for piRNA biogenesis and function. Therefore, polymorphisms in HIWI genes contribute to spermatogenesis defects and can be considered as risk factors for male infertility. The aim of the present study was to investigate the association between the HIWI2 gene rs508485 polymorphism and non-obstructive azoospermia...
April 2017: Reports of Biochemistry & Molecular Biology
Merat Mahmoodi, Maryam Ghodsi, Malihe Moghadam, Mojtaba Sankian
BACKGROUND: The expression of mouse tumor necrosis factor alpha (TNF-α) in Escherichia coli is a favorable way to get high yield of protein; however, the formation of cytoplasmic inclusion bodies, which is the consequence of insoluble accumulated proteins, is a major obstacle in this system. To overcome this obstacle, we used a pulsed dilution method to convert the product to its native conformation. METHODS: Reducing agent and guanidine hydrochloride were used to solubilize inclusion bodies formed after TNF-(α) expression...
April 2017: Reports of Biochemistry & Molecular Biology
Afshin Shirkani, Atena Mansouri, Mohammad Reza Abbaszadegan, Reza Faridhosseini, Farahzad Jabbari Azad, Mehran Gholamin
BACKGROUND: The development of allergic rhinitis (AR) is caused by the interaction between genetic predisposition and environmental factors. In this study, the association between GATA3 single nucleotide polymorphisms and AR in an Iranian population was identified. METHODS: This case-control study was performed on 86 patients with AR and 86 healthy subjects. This study aimed to evaluate a potential association between two GATA3 SNPs, rs1269486 and rs2229360, and AR...
April 2017: Reports of Biochemistry & Molecular Biology
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