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Reports of Biochemistry & Molecular Biology

Fatemeh Keyfi, Abdolreza Varasteh
BACKGROUND: Urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. In the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. Gas chromatography is the most commonly-used technology to separate and identify these metabolites. In this report the analytical conditions for the determination of methylmalonic acid using a gas chromatography/flame ionization detector (GC-FID) are studied with the aim to establish a method to analyze organic acids in human urine...
April 2016: Reports of Biochemistry & Molecular Biology
Hamid Reza Kianifar, Alireza Pourreza, Farahzad Jabbari Azad, Hadis Yousefzadeh, Fatemeh Masomi
BACKGROUND: Diagnosis of food allergy is difficult in children. Food allergies are diagnosed using several methods that include medical histories, clinical examinations, skin prick and serum-specific immunoglobulin E (IgE) tests, radio-allergosorbent test (RAST), food challenge, and supervised elimination diets. In this study we evaluated allergies to cow's milk, egg, peanut, and fish in children with suspected food allergies with skin prick tests and serum and feces RAST. METHODS: Forty-one children with clinical symptoms of food allergies were enrolled in the study...
April 2016: Reports of Biochemistry & Molecular Biology
Bagher Moradi, Mojtaba Sankian, Yousef Amini, Zahra Meshkat
BACKGROUND: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. METHODS: A fusion DNA segment consisting of HspX, linker, PPE44, linker, and EsxV, after codon optimization, was designed...
April 2016: Reports of Biochemistry & Molecular Biology
Mahdi Zahedpanah, Mojgan Shaiegan, Seyed Hamidollah Ghaffari, Mohsen Nikbakht, Mahin Nikugoftar, Saeed Mohammadi
BACKGROUND: Interfering with cell proliferation and survival is a critical role for antineoplastic drugs leading to cell death through induction of apoptosis. Alternative treatments with herbal extracts offer insights into acute myeloid leukemia (AML) therapy. Parthenolide (PTL), an extract from feverfew, induces apoptosis in primary human leukemia stem cells (LSCs) and bulk leukemic cell populations. Osteopontin (OPN) preserves cell viability in response to anticancer agents and its receptors could be utilized for therapeutic targeting of cancer cells...
April 2016: Reports of Biochemistry & Molecular Biology
Masoume Vakili Azghandi, Mohammadreza Nasiri, Ali Shamsa, Mohsen Jalali, Mohammad Mahdi Shariati
BACKGROUND: The SRY gene (SRY) provides instructions for making a transcription factor called the sex-determining region Y protein. The sex-determining region Y protein causes a fetus to develop as a male. In this study, SRY of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences. METHODS: Nucleotide sequences of SRY were retrieved from the NCBI databank...
April 2016: Reports of Biochemistry & Molecular Biology
Mohammed Zaghlool Saeed Al-Khayyat, Ammar Ghanem Ameen Al-Dabbagh
BACKGROUND: LuxI is a component of the quorum sensing signaling pathway in Vibrio fischeriresponsible for the inducer synthesis that is essential for bioluminescence. METHODS: Homology modeling of LuxI was carried out using Phyre2 and refined with the GalaxyWEB server. Five models were generated and evaluated by ERRAT, ANOLEA, QMEAN6, and Procheck. RESULTS: Five refined models were generated by the GalaxyWEB server, with Model 4 having the greatest quality based on the QMEAN6 score of 0...
April 2016: Reports of Biochemistry & Molecular Biology
Mohammad Moshiri, Fatemeh Hamid, Leila Etemad
Seeds of the castor bean plant Ricinuscommunis L (CB) contain ricin toxin (RT), one of the most poisonous naturally-occurring substances known. Ricin toxin, a water-soluble glycoprotein that does not partition into the oil extract, is a ribosome-inactivating toxin composed of two chains, labeled A and B. Severity of the toxicity varies depending on the route of exposure to the toxin. Inhalational is the most toxic route, followed by oral ingestion. Orally-ingested RT accumulates in the liver and spleen but other cells are also affected...
April 2016: Reports of Biochemistry & Molecular Biology
Mehrdad Gholami, Alireza Salimi Chirani, Mona Moshiri, Mansour Sedighi, Abazar Pournajaf, Masoud Tohidfar, Gholamreza Irajian
BACKGROUND: Neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5-15% of adults. The aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type IV pilus biogenesis protein antigen (ACP-MIP-PilQ) from N. meningitidis serogroup B strain. METHODS: Biochemical properties and multiple alignments were predicted by appropriate web servers...
October 2015: Reports of Biochemistry & Molecular Biology
Mohammad Askari, Amin Reza Nikpoor, Fazel Gorjipour, Mohsen Mazidi, Mohammad Hosein Sanati, Hajar Aryan, Alireza Irani, Khalil Ghasemi Falavarjani, Hossein Nazari, Kazem Mousavizadeh
BACKGROUND: Half of the cases of vision loss in people under 60 years of age have been attributed to age-related macular degeneration (AMD). This is a multifactorial disease with late onset. It has been demonstrated that many different genetic loci are implicated in the risk of developing AMD in different populations. In the current study, we investigated the association of high-temperature ‎requirement A-1 (HTRA1) gene polymorphisms with the risk of developing AMD in the Iranian population...
October 2015: Reports of Biochemistry & Molecular Biology
Mansour Sedighi, Mohsen Moghoofei, Ebrahim Kouhsari, Abazar Pournajaf, Behzad Emadi, Masoud Tohidfar, Mehrdad Gholami
BACKGROUND: Sigma factors are proteins that regulate transcription in bacteria. Sigma factors can be activated in response to different environmental conditions. The rpoS (RNA polymerase, sigma S) gene encodes sigma-38 (σ38, or RpoS), a 37.8 kDa protein in Pseudomonas aeruginosa (P. aeruginosa) strains. RpoS is a central regulator of the general stress response and operates in both retroactive and proactive manners; not only does it allow the cell to survive environmental challenges; it also prepares the cell for subsequent stresses (cross-protection)...
October 2015: Reports of Biochemistry & Molecular Biology
Mozhgan Soltani, Kazem Parivar, Javad Baharara, Mohammad Amin Kerachian, Javad Asili
BACKGROUND: Marine natural products contain a wide range of bioactive compounds with therapeutic properties that have revealed crucial properties in the treatment of some diseases. Some of these compounds have recently received considerable attention for drug discovery. In this study we examined the anti-angiogenic effect of saponin isolated from Holothuria leucospilota (sea cucumber) through evaluation of vascular endothelial growth factor D (VEGF-D) and transforming growth factor-β (TGFβ) expression in a breast cancer cell line...
October 2015: Reports of Biochemistry & Molecular Biology
Malihe Moghadam, Ali Ganji, Abdolreza Varasteh, Reza Falak, Mojtaba Sankian
BACKGROUND: Recombinant proteins overexpressed in E. coli are usually deposited in inclusion bodies. Cysteines in the protein contribute to this process. Inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed in E. coli. Hence, aggregated proteins should be solubilized and allowed to refold to obtain native- or correctly- folded recombinant proteins. METHODS: Dilution method that allows refolding of recombinant proteins, especially at high protein concentrations, is to slowly add the soluble protein to refolding buffer...
October 2015: Reports of Biochemistry & Molecular Biology
Mohammadreza Nassiri, Hamid Ariannejad
BACKGROUND: Degradation of phytic acid to inorganic phosphate in domestic animals' diets requires thermostable phytase. Although Basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. METHODS: The phytase gene from Bacillus subtilis DR8886 was isolated from Dig Rostam hot mineral spring in Iran and cloned into pET21(+) and pET32(+)...
October 2015: Reports of Biochemistry & Molecular Biology
Mahdieh Soezi, Arash Memarnejadian, Saeed Aminzadeh, Rezvan Zabihollahi, Seyed Mehdi Sadat, Safieh Amini, Soheila Hekmat, Mohammad Reza Aghasadeghi
BACKGROUND: The rapid increase of HIV-1 strains resistant to current antiretroviral drugs is a challenge for successful AIDS therapy. This necessitates the development of novel drugs, and to this end, availability of screening systems for in vitro drug discovery is a priority. Herein, we report the modification of a previously developed system for increased sensitivity, ease of use, and cost-efficiency, based on the application of the EGFP marker. METHODS: A PCR-amplified gfp gene (gfp) was cloned into pmzNL4-3, the plasmid already designed to produce single-cycle replicable virions, in frame with the reverse-transcriptase gene to construct the pmzNL4-3/GFP plasmid...
October 2015: Reports of Biochemistry & Molecular Biology
Masoumeh Malek-Jafarian, Fatemeh-Sadat Hosseini, Abodol-Reza Ahmadi
BACKGROUND: One of the main causes of sexually transmitted diseases is group B β- hemolytic streptococci (GBS) multiplying in the genital tracts. Penicillin is the most common drug for the treatment of infections caused by these bacteria, but in patients suffering from Penicillin allergy, Erythromycin and Clindamycin are used as alternative therapeutic drugs against GBS. Recently, resistance to these drugs has been reported more often. In this study, efforts have been made to determine the prevalence and antibiotic resistance of GBS...
April 2015: Reports of Biochemistry & Molecular Biology
Mohammad Reza Khakzad, Farhad Salari, Maryam Javanbakht, Maryam Hojati, Abdolreza Varasteh, Mojtaba Sankian, Mojtaba Meshkat
BACKGROUND: Transforming growth factor-β1 (TGF-β1) has been found to play a crucial role in early central nervous system development. Several studies have illustrated decreased TGF-β1 levels in sera and brains of autistic children. Two point mutations in the TGF-β1 signal peptide at 869T/C and 915G/C have been reported to influence TGF-β1 expression. The aim of the present study was to investigate the correlation of TGF-β1 polymorphisms and their haplotypes with autism. METHODS: This study was performed on 39 autistic patients and 35 age- and sex-matched normal controls in an Iranian population, using the sequence specific primed-polymerase chain reaction (PCR-SSP) technique...
April 2015: Reports of Biochemistry & Molecular Biology
Mahnaz Mahmoudi Rad, Niki Mahmoudi Rad, Yasaman Mirdamadi
BACKGROUND: The multifunctional transforming growth factor beta (TGF-β) is a glycoprotein that exists in three isoforms. TGF-β3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key cells in the wound healing process. TGF-β3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process...
April 2015: Reports of Biochemistry & Molecular Biology
Javad Baharara, Elaheh Amini, Marzieh Mousavi
BACKGROUND: Anti-angiogenic therapy is a crucial step in cancer treatment. The discovery of new anti-angiogenic compounds from marine organisms has become an attractive concept in anti-cancer therapy. Because little data correlated to the pro- and anti-angiogenic efficacies of Ophiuroidea, which include brittle star, the current study was designed to explore the anti-angiogenic potential of brittle star methanol extract in vitro and in vivo. METHODS: The anti-proliferative effect of brittle star extract on A2780cp cells was examined by MTT assays, and transcriptional expression of VEGF and b-FGF was evaluated by RT-PCR...
April 2015: Reports of Biochemistry & Molecular Biology
Zahra Samadi Noshahr, Mohammad Reza Shahraki, Hassan Ahmadvand, Davood Nourabadi, Alireza Nakhaei
BACKGROUND: We investigated the effects of Withania somnifera root (WS) on insulin resistance, tumor necrosis factor α (TNF-α), and interleukin-6 (IL-6) in fructose-fed rats. METHODS: Forty-eight Wistar-Albino male rats were randomly divided into four groups (n=12); Group I as control, Group II as sham-treated with WS by 62.5mg/g per diet, Group III fructose-fed rats received 10%W/V fructose, and Group IV fructose- and WS-fed rats. After eight weeks blood samples were collected to measure glucose, insulin, IL-6, and TNF-α levels in sera...
April 2015: Reports of Biochemistry & Molecular Biology
Rohollah Taghadosi, Mohammad Reza Shakibaie, Shalaleh Masoumi
BACKGROUND: N-Acyl homoserine lactone (AHL) is found to be the main component of quorum sensing (QS) in Gram-negative bacteria and plays an important role in biofilm formation. Little information is available regarding the role of AHL in biofilm formation in Escherichia coli (E. coli). The purpose of this investigation was to biochemically detect and characterize AHL activity in biofilm-forming uropathogenic E. coli (UPEC) isolated from urine samples of the patients with urinary tract infections (UTIs) in Kerman, Iran...
April 2015: Reports of Biochemistry & Molecular Biology
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