Sylvain Engilberge, Nicolas Caramello, Sergei Bukhdruker, Martin Byrdin, Thierry Giraud, Philippe Jacquet, Damien Scortani, Rattana Biv, Hervé Gonzalez, Antonin Broquet, Peter van der Linden, Samuel L Rose, David Flot, Taras Balandin, Valentin Gordeliy, J Mia Lahey-Rudolph, Manfred Roessle, Daniele de Sanctis, Gordon A Leonard, Christoph Mueller-Dieckmann, Antoine Royant
The technique of time-resolved macromolecular crystallography (TR-MX) has recently been rejuvenated at synchrotrons, resulting in the design of dedicated beamlines. Using pump-probe schemes, this should make the mechanistic study of photoactive proteins and other suitable systems possible with time resolutions down to microseconds. In order to identify relevant time delays, time-resolved spectroscopic experiments directly performed on protein crystals are often desirable. To this end, an instrument has been built at the icOS Lab (in crystallo Optical Spectroscopy Laboratory) at the European Synchrotron Radiation Facility using reflective focusing objectives with a tuneable nanosecond laser as a pump and a microsecond xenon flash lamp as a probe, called the TR-icOS (time-resolved icOS) setup...
January 1, 2024: Acta Crystallographica. Section D, Structural Biology