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Iranian Journal of Veterinary Research

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https://www.readbyqxmd.com/read/27822254/relationship-between-white-spot-syndrome-virus-wssv-loads-and-characterizations-of-water-quality-in-litopenaeus-vannamei-culture-ponds-during-the-tropical-storm
#1
J S Zhang, Z J Li, G L Wen, Y L Wang, L Luo, H J Zhang, H B Dong
An in-situ experiment was conducted to investigate the effect of tropical storm on the white spot syndrome virus (WSSV) loads in Litopenaeus vannamei rearing ponds. White spot syndrome virus loads, heterotrophic bacteria, Vibrio and water quality (including temperature, dissolved oxygen (DO), salinity, pH, NH4-N, and NO2-N) were continually monitored through one tropical storm. The WSSV loads decreased when tropical storm made landfall, and substantially increased when typhoon passed. The variation of WSSV loads was correlated with DO, temperature, heterotrophic bacteria count, and ammonia-N concentrations...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822253/estrogen-receptor-gene-1-expression-in-caprine-and-its-effect-on-fertility
#2
S Saraswat, P K Rout, S D Kharche, A K Goel, S K Jindal, S Kumar
The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of ESR1 gene was analyzed by RT-PCR (Roche LC-480). Relative quantification by RT-PCR indicated that the ESR1 gene expression showed more fold in fertile bucks as compared to non-fertile...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822252/effects-of-hydro-alcoholic-extract-of-vitex-agnus-castus-fruit-on-kidney-of-d-galactose-induced-aging-model-in-female-mice
#3
A A Oroojan, A Ahangarpour, L Khorsandi, S A Najimi
The aim of the present study was to evaluate the effect of a hydro-alcoholic extract of Vitex agnus-castus (VAC) fruit on blood urea nitrogen (BUN), creatinine (Cr) and, kidney histology of a female mouse model of D-galactose induced aging. In this experimental study, 72 NMRI mice were divided into 6 groups: control, VAC, D-galactose, D-galactose+VAC, aging, and aging+VAC. D-galactose was injected for 45 days and, VAC extract administered in the last 7 days, twice a day. Serum BUN and Cr levels were not significantly changed in the D-galactose and natural aged animals in comparison to control group...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822251/immunostimulatory-effect-of-the-aqueous-leaf-extract-of-phyllanthus-niruri-on-the-specific-and-nonspecific-immune-responses-of-oreochromis-mossambicus-peters
#4
M Muthulakshmi, P A Subramani, R D Michael
Plant derived immunostimulants are a promising alternative to chemotherapeutics and also perhaps vaccines. In the present study, we examined the immunostimulating properties of aqueous leaf extract of Phyllanthus niruri, an Indian traditional medicinal herb, on neutrophil activation and antibody response of Oreochromis mossambicus (Peters). Serial ten-fold diluted doses of P. niruri ranging from 0.002 mg to 20 mg were administered to two groups of O. mossambicus (n=8). One group of fishes was administered with sheep red blood cells and the primary and secondary antibody responses were estimated using direct haemagglutination assay...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822250/mitochondrial-dna-sequence-based-phylogenetic-relationship-of-trichiurus-lepturus-perciformes-trichiuridae-from-the-persian-gulf
#5
S Tamadoni Jahromi, S A Mohd Noor, K Pirian, R Dehghani, M Nazemi, A Khazaali
In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship of Trichiurus lepturus in the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs to T. lepturus showing 42 divergence sites among the same reported partial sequences of 16S rRNA gene from the other area (West Atlantic and Indo-Pacific area). Phylogeny results showed that all 18 haplotypes of the species clustered into five clades with reasonably high bootstrap support of values (>64%)...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822249/the-influence-of-administration-of-different-doses-of-arginine-and-lysine-coupled-with-zinc-in-the-lactating-ewes-on-the-zinc-concentration-in-milk
#6
M Keshvari, H Khazali, H Rokni, A Hosseini
Zinc (Zn) plays an essential role in the human and animal body system. Zinc deficiency might cause many disorders, so it is important to provide a sufficient amount of this microelement in both animals and humans. One of the most important sources of Zn is milk. The purpose of this study was to determine whether Zn can pass through the mammary epithelial cell into milk by a co-transport system with amino acid in the lactating ewes. This experimental research included 54 lactating ewes collected from the Agriculture-Jahad Organization of Tehran Province, that were allocated into 9 groups as follows: groups which received inorganic form as Zn chloride (1, 2 and 4 mg/kg), and groups which received organic Zn with different doses of arginine or lysine (1, 2 and 4 mg/kg) in chelate form...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822248/proteomic-analysis-of-muscle-tissue-from-rainbow-trout-oncorhynchus-mykiss-fed-dietary-%C3%AE-glucan
#7
Gh Ghaedi, S Keyvanshokooh, H Mohammadi Azarm, M Akhlaghi
The aim of this study was to examine the changes in muscle proteome of the rainbow trout fed dietary β-glucan. The experimental diets contained 0 (control), 0.1% and 0.2% β-1,3/1,6 yeast glucan. First, feeding larvae were fed to apparent satiation nine times per day with their respective diets over two months. The percentage of body weight gain and feed efficiency of fish fed 0.2% diet was significantly higher than those of fish fed the control and 0.1% diets. Fish fed the control and 0.2% diets were subjected to proteomic analysis...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822247/pfge-genotyping-and-molecular-characterization-of-campylobacter-spp-isolated-from-chicken-meat
#8
B Bakhshi, M Kalantar, A Rastegar-Lari, F Fallah
A total of 70 samples were collected from chicken meat obtained from 10 markets in Tehran, Iran from which 39 Campylobacter coli were isolated. Among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (SXT) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). No resistance was to gentamycin was observed. None of the Campylobacter strains under study harbored integron, suggesting the involvement of other resistance mechanisms in emergence of multi drug resistance (MDR) phenotype among the isolates...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822246/evaluating-a-murine-model-of-endometritis-using-uterine-isolates-of-escherichia-coli-from-postpartum-buffalo
#9
S H Dar, S Qureshi, M Palanivelu, S Muthu, S Mehrotra, M H Jan, G R Chaudhary, H Kumar, R Saravanan, K Narayanan
Ascending infection of the uterus with Gram-negative bacteria is responsible for postpartum endometritis in cattle and buffalo and can adversely affect fertility. Development of a laboratory animal model for bovine endometritis would facilitate the understanding of the pathogenesis as it is difficult to conduct controlled experimentation in the native host. In the present study, 30 virgin Swiss Albino mice (5-8 weeks old) were used to evaluate the pathogenic potential of Escherichia coli, isolated from the normally calved postpartum buffalo to induce endometritis...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822245/effects-of-cysteamine-during-in-vitro-maturation-on-viability-and-meiotic-competence-of-vitrified-buffalo-oocytes
#10
K Gh M Mahmoud, M M M El-Sokary, M M M Kandiel, M E A Abou El-Roos, G M S Sosa
The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium with no cysteamine (control) or 50 μM cysteamine (treated). Oocytes were vitrified in vitrification solution 1 (VS1): 1...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822244/genomic-diversity-of-clostridium-perfringens-strains-isolated-from-food-and-human-sources
#11
A Afshari, A Jamshidi, J Razmyar, M Rad
Clostridium perfringens is a serious pathogen which causes enteric diseases in domestic animals and food poisoning in humans. Spores can survive cooking processes and play an important role in the possible onset of disease. In this study, RAPD-PCR and REP-PCR were used to examine the genetic diversity of 49 isolates of C. perfringens type A from three different sources. The results of RAPD-PCR revealed the most genetic diversity among poultry isolates, while human isolates showed the least genetic diversity...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822243/screening-the-partial-coding-region-of-metallothionein-isoform-2-gene-in-zebu-cattle
#12
P N Gholap, D S Kale, K Krishnamurthi, A R Sirothia, M D Kothekar
Metallothionein (MT) is important because it binds tightly to heavy metals to decrease their toxicity. DNA was isolated from 30 toxic metal exposed and 30 toxic metal unexposed Zebu cows. The amplified metallothionein isoform-2 (MT-2) PCR product (489 bp) was further used for PCR-RFLP and DNA sequencing. MT-2 TaqI PCR-RFLP revealed homozygous genotype (AA) except for the E23 animal (AB). The genotype frequency of AA and AB (E23) genotypes in the exposed groups was 0.967 and 0.033 respectively. DNA sequencing was carried out for the toxic metal exposed sample (E23) and the control group sample (C13)...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822242/an-outbreak-of-yolk-sac-infection-and-dead-in-shell-mortality-in-common-canary-serinus-canaria-caused-by-klebsiella-pneumoniae
#13
J Razmyar, A H Zamani
Yolk sac infection (YSI) and dead-in-shell mortality caused by Enterobacteriaceae in birds are not a rare phenomenon, however there are only a few reports indicating the association between these conditions and Klebsiella spp. among canary chicks (Serinus canaria). There have been reports of high mortality among 1-3 day old canary chicks in an indoor flock of canaries. In order to study the causative agent, yolk sac samples from dead-in-shell and day-old canary chicks were cultured. Klebsiella pneumonia was isolated and identified based on biochemical tests and using genus and species-specific multiplex PCR and later tested for their susceptibility to 13 antimicrobial agents...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822241/surgical-management-of-extensive-oral-tumors-by-bilateral-rostral-mandibulectomy-in-two-dogs
#14
M Shafiuzama, M S Sabarish Babu, N Krishnaveni, P Manoj Prabhakar, S Hemalatha, N J D Souza
Two dogs of different age groups were presented with the history of oral growth, bleeding, excessive salivation, bad breath, inappetance and dysphagia. Physical examination revealed cauliflower like reddish growth in the gingiva enclosing the mandibular incisiors and canine tooth. Haematology and serum biochemical profiles were within the normal range. Plain radiography revealed extensive growth involving the lower mandible and gingiva. Hence rostral mandibulectomy was performed to excise the tumor mass. The dogs recovered uneventfully after surgery with no difficulty in feeding liquid and semisolid diet...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822240/immunohistochemical-characterization-of-sebaceous-epithelioma-in-two-dogs
#15
J S Yoon, J Park
This report describes two cases of sebaceous epithelioma and its immunohistochemical characterization with CK 14, CK18, p63, Ki67 and Bcl-2 immunostaining. Case 1 was a 12-year-old, intact English Cocker spaniel female presenting with multiple skin nodules over one year. Case 2 was a 7-year-old, spayed poodle female with a five-month history of solitary mass. Hematoxylin and eosin (H&E) staining showed that the nodules in both cases were irregular lobules comprised of epithelial cells around well-differentiated sebocytes...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822239/first-report-of-miru-vntr-genotyping-of-mycobacterium-avium-subsp-paratuberculosis-isolates-from-egypt
#16
A Fawzy, A Fayed, H Youssef, A El-Sayed, M Zschöck
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) were carried out in different countries. Until now there is no published information about MIRU-VNTR genotyping of MAP isolates in Egypt...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822238/efficacy-of-repeated-doses-of-diminazene-aceturate-dinazene-%C3%A2-in-the-treatment-of-experimental-trypanosoma-brucei-infection-of-albino-rats
#17
I O Ezeh, E N Ugwu, O V Enemuo, C F Obi, C N Iheagwam, R C Ezeokonkwo, D N Onah
The efficacy of repeated doses of Dinazene(®) in Albino rats experimentally infected with Trypanosoma brucei (Gboko strain) was investigated. A total of 30 adult female Albino rats weighing 130-190 g were used for the study. They were assigned to six groups (groups A-F) of five rats each. Groups A-D were infected intraperitoneally with 1.0 × 10(6) trypanosomes in 400 µL of PBS diluted blood while groups E (uninfected treated) and F (uninfected untreated) served as controls. The rats in the groups A-D as well as those in group E were treated with 7...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822237/the-superiority-of-paracostal-endoscopic-assisted-gastropexy-over-open-incisional-and-belt-loop-gastropexy-in-dogs-a-comparison-of-three-prophylactic-techniques
#18
A Tavakoli, M Mahmoodifard, A H Razavifard
Prophylactic gastropexy is a procedure that prevents the occurrence of a life threatening condition known as gastric dilation and volvulus (GDV) in dogs. The objective of this study was to compare incisional, belt loop and minimally invasive endoscopically assisted gastropexy by evaluating different parameters such as surgical time, length of scar and score of pain in dogs. Twenty-one healthy, mixed-breed adult dogs weighting 14.3 ± 2.6 kg were randomly divided into three groups. Three gastropexy techniques applied in the following order: incisional (group I), belt loop (group B), and endoscopically assisted gastropexy (group E)...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822236/effect-of-substituting-increasing-levels-of-organic-zn-for-inorganic-zn-on-performance-hematological-and-serum-biochemical-constituents-antioxidant-status-and-immune-response-in-rat
#19
D Nagalakshmi, K Sridhar, P S Swain, A G Reddy
The effect of replacing dietary Zn supplemented from inorganic (ZnCO3) source with organic Zn (Zn methionine; Zn-met) was investigated in 72 rats (98.42 ± 1.483 g) by randomly allotting to 4 diets (6 replicates/diet, 3 rats/replicate). Basal diet was prepared with purified ingredients without Zn. The control diet (AIN-76A) contained 12 ppm of Zn from ZnCO3 (100-I). In the other diets ZnCO3 was replaced with Zn-met at the rates of 50 (50I:50O), 75 (25I:75O) or 100% (100-O). Weekly body weight and daily feed intake were recorded for 14 weeks...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27822235/development-of-anti-helicobacter-pylori-immunoglobulins-y-igys-in-quail
#20
S Najdi, G Nikbakht Brujeni, N Sheikhi, S Chakhkar
Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic bacterium that cause the stomach infection in more than 50% of human population worldwide. The aim of this study was to examine the possibility of anti-H. pylori immunoglobulins Y (IgYs) production in quails and evaluate the effect of the different methods of immunization on titers of IgY in egg yolks. Whole cell bacterial antigen was used for immunization of quails. Forty Japanese quails (Coturnixjaponica) were divided into four groups. The first group intramuscularly immunized with one dose of antigen (3 × 10(8) inactivated bacteria) whereas the second group injected with half dose...
2016: Iranian journal of veterinary research
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