Mattias Van Heetvelde, Wouter Van Loocke, Wim Trypsteen, Annelot Baert, Katrien Vanderheyden, Brecht Crombez, Jo Vandesompele, Kim De Leeneer, Kathleen B M Claes
INTRODUCTION: For the relative quantification of isoform expression, RT-qPCR has been the gold standard for over a decade. More recently, digital PCR is becoming widely implemented, as it is promised to be more accurate, sensitive and less affected by inhibitors, without the need for standard curves. In this study we evaluated RT-qPCR versus RT-droplet digital PCR (ddPCR) for the relative quantification of isoforms in controls and carriers of the splice site mutation BRCA1 c.212+3A>G, associated with increased expression of several isoforms...
September 2017: Biomolecular Detection and Quantification