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Bioresources and Bioprocessing

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https://www.readbyqxmd.com/read/28913159/enzymatic-characterization-of-a-recombinant-carbonyl-reductase-from-acetobacter-sp-cctcc-m209061
#1
Ping Wei, Yu-Han Cui, Min-Hua Zong, Pei Xu, Jian Zhou, Wen-Yong Lou
BACKGROUND: Acetobacter sp. CCTCC M209061 could catalyze carbonyl compounds to chiral alcohols following anti-Prelog rule with excellent enantioselectivity. Therefore, the enzymatic characterization of carbonyl reductase (CR) from Acetobacter sp. CCTCC M209061 needs to be investigated. RESULTS: A CR from Acetobacter sp. CCTCC M209061 (AcCR) was cloned and expressed in E. coli. AcCR was purified and characterized, finding that AcCR as a dual coenzyme-dependent short-chain dehydrogenase/reductase (SDR) was more preferred to NADH for biocatalytic reactions...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28890864/germ-soak-water-as-nutrient-source-to-improve-fermentation-of-corn-grits-from-modified-corn-dry-grind-process
#2
Ankita Juneja, Deepak Kumar, Vijay Singh
Corn fractionation in modified dry grind processes results in low fermentation efficiency of corn grits because of nutrient deficiency. This study investigated the use of nutrient-rich water from germ soaking to improve grits fermentation in the conventional dry grind and granular starch hydrolysis (GSH) processes. Comparison of germ soak water with the use of protease and external B-vitamin addition in improving grits fermentation was conducted. Use of water from optimum soaking conditions (12 h at 30 °C) resulted in complete fermentation with 29 and 8% higher final ethanol yields compared to that of control in conventional and GSH process, respectively...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28845382/enhancing-transglutaminase-production-of-streptomyces-mobaraensis-by-iterative-mutagenesis-breeding-with-atmospheric-and-room-temperature-plasma-artp
#3
Ying Jiang, Yue-Peng Shang, Hao Li, Chao Zhang, Jiang Pan, Yun-Peng Bai, Chun-Xiu Li, Jian-He Xu
OBJECTIVES: To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production. RESULTS: After eight rounds of iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, and Sm7-V12, were identified, which showed increased TGase production by 27, 24, 24, and 19%, respectively...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28824835/photocontrolled-reversible-self-assembly-of-dodecamer-nitrilase
#4
Qiao Yu, Yong Wang, Shengyun Zhao, Yuhong Ren
BACKGROUND: Naturally photoswitchable proteins act as a powerful tool for the spatial and temporal control of biological processes by inducing the formation of a photodimerizer. In this study, a method for the precise and reversible inducible self-assembly of dodecamer nitrilase in vivo (in Escherichia coli) and in vitro (in a cell-free solution) was developed by means of the photoswitch-improved light-inducible dimer (iLID) system which could induce protein-protein dimerization. RESULTS: Nitrilase was fused with the photoswitch protein AsLOV2-SsrA to achieve the photocontrolled self-assembly of dodecamer nitrilase...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28804701/application-of-methanol-and-sweet-potato-vine-hydrolysate-as-enhancers-of-citric-acid-production-by-aspergillus-niger
#5
Daobing Yu, Yanke Shi, Qun Wang, Xin Zhang, Yuhua Zhao
BACKGROUND: Agricultural waste is as an alternative low-cost carbon source or beneficial additives which catch most people's eyes. In addition, methanol and sweet potato vine hydrolysate (SVH) have been reported as the efficient enhancers of fermentation according to some reports. The objective of the present study was to confirm SVH as an efficient additive in CA production and explore the synergistic effects of methanol and SVH in fermentation reactions. RESULTS: The optimal fermentation conditions resulted in a maximum citric acid concentration of 3...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28794956/recent-progress-on-deep-eutectic-solvents-in-biocatalysis
#6
REVIEW
Pei Xu, Gao-Wei Zheng, Min-Hua Zong, Ning Li, Wen-Yong Lou
Deep eutectic solvents (DESs) are eutectic mixtures of salts and hydrogen bond donors with melting points low enough to be used as solvents. DESs have proved to be a good alternative to traditional organic solvents and ionic liquids (ILs) in many biocatalytic processes. Apart from the benign characteristics similar to those of ILs (e.g., low volatility, low inflammability and low melting point), DESs have their unique merits of easy preparation and low cost owing to their renewable and available raw materials...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28752021/mycoremediation-potential-of-pleurotus-species-for-heavy-metals-a-review
#7
REVIEW
Meena Kapahi, Sarita Sachdeva
Mycoremediation is one of the biotechniques that recruits fungi to remove toxic pollutants from environment in an efficient and economical manner. Mushrooms, macro-fungi, are among the nature's most important mycoremediators. Pleurotus species (also called oyster mushrooms) are considered to be the most popular and widely cultivated varieties worldwide and this might be attributed to their low production cost and higher yields. Apart from their nutritive and therapeutic properties, Pleurotus species have high biosorption potential due to their extensive biomass, i...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28748136/study-on-community-structure-of-microbial-consortium-for-the-degradation-of-viscose-fiber-wastewater
#8
Chao-Qun Ding, Kun-Rong Li, Yun-Xia Duan, Shi-Ru Jia, He-Xin Lv, He Bai, Cheng Zhong
BACKGROUND: Enrichment culture was applied to obtain microbial consortium from activated sludge samples collected from biodegradation system, a chemical fiber plant in Hebei Province, China. Bacterial composition and community dynamic variation were assessed employing denaturing gradient gel electrophoresis fingerprinting technology based on amplified 16S rRNA genes in the entire process of enrichment culture for viscose fiber wastewater. RESULTS: Four bacteria named as VF01, VF02, VF03, and VF04 were isolated from the microbial consortium adopting the spray-plate method...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28736695/comparative-evaluation-of-wastewater-treatment-microbial-fuel-cells-in-terms-of-organics-removal-waste-sludge-production-and-electricity-generation
#9
Yusuke Asai, Morio Miyahara, Atsushi Kouzuma, Kazuya Watanabe
Microbial fuel cells (MFCs) are devices that exploit living microbes for electricity generation coupled to organics degradation. MFCs are expected to be applied to energy-saving wastewater treatment (WWT) as alternatives to activated-sludge reactors (ASRs). Although extensive laboratory studies have been performed to develop technologies for WWT-MFCs, limited information is available for comparative evaluation of MFCs and ASRs in terms of organics removal and waste-sludge production. In the present study, laboratory WWT experiments were performed using cassette-electrode MFCs and ASRs that were continuously supplied either with artificial domestic wastewater (ADW) containing starch and peptone or with artificial industrial wastewater (AIW) containing methanol as the major organic matter...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28736694/biochemical-properties-of-a-new-thermo-and-solvent-stable-xylanase-recovered-using-three-phase-partitioning-from-the-extract-of-bacillus-oceanisediminis-strain-sj3
#10
Nawel Boucherba, Mohammed Gagaoua, Amel Bouanane-Darenfed, Cilia Bouiche, Khelifa Bouacem, Mohamed Yacine Kerbous, Yacine Maafa, Said Benallaoua
The present study investigates the production and partial biochemical characterization of an extracellular thermostable xylanase from the Bacillus oceanisediminis strain SJ3 newly recovered from Algerian soil using three phase partitioning (TPP). The maximum xylanase activity recorded after 2 days of incubation at 37 °C was 20.24 U/ml in the presence of oat spelt xylan. The results indicated that the enzyme recovered in the middle phase of TPP system using the optimum parameters were determined as 50% ammonium sulfate saturation with 1...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28725525/microbial-degradation-of-petrochemical-waste-polycyclic-aromatic-hydrocarbons
#11
M H Fulekar
BACKGROUND: Petrochemical industry is one of the fastest growing industries. This industry has immense importance in the growth of economy and manufacture of large varieties of chemicals. The petrochemical industry is a hazardous group of industry generating hazardous waste containing organic and inorganic compounds. In spite of the present treatment process, the hazardous waste compounds are found untreated to the acceptable level and found discharged at soil-water environment resulting into the persistent organic-inorganic pollutant into the environment...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28680802/lobster-processing-by-products-as-valuable-bioresource-of-marine-functional-ingredients-nutraceuticals-and-pharmaceuticals
#12
REVIEW
Trung T Nguyen, Andrew R Barber, Kendall Corbin, Wei Zhang
The worldwide annual production of lobster was 165,367 tons valued over $3.32 billion in 2004, but this figure rose up to 304,000 tons in 2012. Over half the volume of the worldwide lobster production has been processed to meet the rising global demand in diversified lobster products. Lobster processing generates a large amount of by-products (heads, shells, livers, and eggs) which account for 50-70% of the starting material. Continued production of these lobster processing by-products (LPBs) without corresponding process development for efficient utilization has led to disposal issues associated with costs and pollutions...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28680801/detoxification-of-parthenium-parthenium-hysterophorus-and-its-metamorphosis-into-an-organic-fertilizer-and-biopesticide
#13
Naseer Hussain, Tasneem Abbasi, Shahid Abbas Abbasi
BACKGROUND: Vermicompost of the toxic and allelopathic weed parthenium (Parthenium hysterophorus) was explored for its possible use as an organic fertilizer. Replicated plant growth trials were conducted using four levels of parthenium vermicompost (0, 2.5, 3.75, and 5 t/ha) to assess their effects on the germination, growth, and fruition of a typical food plant ladies finger (Abelmoschus esculentus). Additionally the role of vermicompost in reducing plant pests and disease was evaluated...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28680800/beechwood-carbohydrates-for-enzymatic-synthesis-of-sustainable-glycolipids
#14
Sascha Siebenhaller, Tatjana Hajek, Claudia Muhle-Goll, Miriam Himmelsbach, Burkhard Luy, Frank Kirschhöfer, Gerald Brenner-Weiß, Thomas Hahn, Susanne Zibek, Christoph Syldatk
Moving away from crude oil to renewable resources for the production of a wide range of compounds is a challenge for future generations. To overcome this, the use of lignocellulose as substrate can contribute to drastically reduce the consumption of crude oil. In this study, sugars from lignocellulose were used as a starting material for the enzymatic synthesis of surface-active sugar esters. The substrates were obtained by an acid-catalyzed, beechwood pretreatment process, which resulted in a fiber fraction that is subsequently hydrolyzed to obtain the monosaccharides...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28596935/green-synthesis-of-enzyme-metal-organic-framework-composites-with-high-stability-in-protein-denaturing-solvents
#15
Xiaoling Wu, Cheng Yang, Jun Ge
OBJECTIVES: Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study. RESULTS: Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28580232/mineralization-of-a-sulfonated-textile-dye-reactive-red-31-from-simulated-wastewater-using-pellets-of-aspergillus-bombycis
#16
Razia Khan, M H Fulekar
BACKGROUND: Reactive Red 31, applied extensively in the commercial textile industry, is a hazardous and persistent azo dye compound often present in dye manufacturing and textile industrial effluents. Aspergillus bombycis strain was isolated from dye contaminated zones of Gujarat Industrial Development Corporation, Vatva, Ahmedabad, India. The decolorization potential was monitored by the decrease in maximum absorption of the dye using UV-visible spectroscopy. Optimization of physicochemical conditions was carried out to achieve maximum decolorization of Reactive Red 31 by fungal pellets...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28546903/comprehensive-reconstruction-and-evaluation-of-pichia-pastoris-genome-scale-metabolic-model-that-accounts-for-1243-orfs
#17
Rui Ye, Mingzhi Huang, Hongzhong Lu, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang, Siliang Zhang
BACKGROUND: Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P. pastoris metabolism. METHODS: In this paper, we upgraded P. pastoris genome-scale metabolic model based on the combination of latest genome annotations and literatures. Then the performance of the new model was evaluated using the Cobra Toolbox v2...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28275533/biofibres-from-biofuel-industrial-byproduct-pongamia-pinnata-seed-hull
#18
Puttaswamy Manjula, Govindan Srinikethan, K Vidya Shetty
BACKGROUND: Biodiesel production using Pongamia pinnata (P. pinnata) seeds results in large amount of unused seed hull. These seed hulls serve as a potential source for cellulose fibres which can be exploited as reinforcement in composites. METHODS: These seed hulls were processed using chlorination and alkaline extraction process in order to isolate cellulose fibres. Scanning electron microscopy (SEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) analysis demonstrated the morphological changes in the fibre structure...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28261538/efficient-expression-of-sortase-a-from-staphylococcus-aureus-in-escherichia-coli-and-its-enzymatic-characterizations
#19
Zhimeng Wu, Haofei Hong, Xinrui Zhao, Xun Wang
BACKGROUND: Sortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (ranging from several milligrams to 76.9 mg/L at most). The present study aims to optimize fermentation conditions for improving SrtA expression in E. coli. RESULTS: Under the optimized media (0.48 g/L glycerol, 1.37 g/L tryptone, 0.51 g/L yeast extract, MOPS 0...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28251042/techno-economic-analysis-of-extraction-based-separation-systems-for-acetone-butanol-and-ethanol-recovery-and-purification
#20
Víctor Hugo Grisales Díaz, Gerard Olivar Tost
BACKGROUND: Dual extraction, high-temperature extraction, mixture extraction, and oleyl alcohol extraction have been proposed in the literature for acetone, butanol, and ethanol (ABE) production. However, energy and economic evaluation under similar assumptions of extraction-based separation systems are necessary. Hence, the new process proposed in this work, direct steam distillation (DSD), for regeneration of high-boiling extractants was compared with several extraction-based separation systems...
2017: Bioresources and Bioprocessing
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