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Cell Discovery

Hongduo Sun, Jiawei Wang, Zhaohui Gong, Jiaying Yao, Yuangao Wang, Jian Xu, Guo-Cheng Yuan, Yijing Zhang, Zhen Shao
No abstract text is available yet for this article.
2018: Cell Discovery
Xuhua Zhang, Linping Xu, Ruihua Fan, Quanli Gao, Yunfeng Song, Xiaodong Lyu, Jiangtao Ren, Yongping Song
Cpf1, an RNA-guided DNA endonuclease that belongs to a new class II CRISPR system, has recently been harnessed for genome editing. Herein, we report an RNase-resistant caged truncated pre-tRNA-like crRNA (catRNA) that confers precise and efficient gene editing with the Lachnospiraceae bacterium Cpf1 (LbCpf1) and enables the reprogramming of catalytically dead LbCpf1 (dCpf1) lacking DNA endonuclease activity into a transcriptional modulator. Specific gene knock-outs and knock-ins were increased 3.2-fold and 4...
2018: Cell Discovery
Svitlana Melnik, Dmytro Dvornikov, Karin Müller-Decker, Sofia Depner, Peter Stannek, Michael Meister, Arne Warth, Michael Thomas, Tomas Muley, Angela Risch, Christoph Plass, Ursula Klingmüller, Christof Niehrs, Andrey Glinka
Use of the diabetes type II drug Metformin is associated with a moderately lowered risk of cancer incidence in numerous tumor entities. Studying the molecular changes associated with the tumor-suppressive action of Metformin we found that the oncogene SOX4 , which is upregulated in solid tumors and associated with poor prognosis, was induced by Wnt/β-catenin signaling and blocked by Metformin. Wnt signaling inhibition by Metformin was surprisingly specific for cancer cells. Unraveling the underlying specificity, we identified Metformin and other Mitochondrial Complex I (MCI) inhibitors as inducers of intracellular acidification in cancer cells...
2018: Cell Discovery
Shujing Lai, Wentao Huang, Yang Xu, Mengmeng Jiang, Haide Chen, Chen Cheng, Yingru Lu, He Huang, Guoji Guo, Xiaoping Han
No abstract text is available yet for this article.
2018: Cell Discovery
Yuli Li, Ruijia Wang, Xiaogang Xun, Jing Wang, Lisui Bao, Ramesha Thimmappa, Jun Ding, Jingwei Jiang, Liheng Zhang, Tianqi Li, Jia Lv, Chuang Mu, Xiaoli Hu, Lingling Zhang, Jing Liu, Yuqiang Li, Lijie Yao, Wenqian Jiao, Yangfan Wang, Shanshan Lian, Zelong Zhao, Yaoyao Zhan, Xiaoting Huang, Huan Liao, Jia Wang, Hongzhen Sun, Xue Mi, Yu Xia, Qiang Xing, Wei Lu, Anne Osbourn, Zunchun Zhou, Yaqing Chang, Zhenmin Bao, Shi Wang
Echinoderms exhibit several fascinating evolutionary innovations that are rarely seen in the animal kingdom, but how these animals attained such features is not well understood. Here we report the sequencing and analysis of the genome and extensive transcriptomes of the sea cucumber Apostichopus japonicus , a species from a special echinoderm group with extraordinary potential for saponin synthesis, aestivation and organ regeneration. The sea cucumber does not possess a reorganized Hox cluster as previously assumed for all echinoderms, and the spatial expression of Hox7 and Hox11/13b potentially guides the embryo-to-larva axial transformation...
2018: Cell Discovery
Dang Vinh Do, Bernhard Strauss, Engin Cukuroglu, Iain Macaulay, Keng Boon Wee, Tim Xiaoming Hu, Ruiz De Los Mozos Igor, Caroline Lee, Andrew Harrison, Richard Butler, Sabine Dietmann, Ule Jernej, John Marioni, Christopher W J Smith, Jonathan Göke, M Azim Surani
The RNA-binding protein SRSF3 (also known as SRp20) has critical roles in the regulation of pre-mRNA splicing. Zygotic knockout of Srsf3 results in embryo arrest at the blastocyst stage. However, SRSF3 is also present in oocytes, suggesting that it might be critical as a maternally inherited factor. Here we identify SRSF3 as an essential regulator of alternative splicing and of transposable elements to maintain transcriptome integrity in mouse oocyte. Using 3D time-lapse confocal live imaging, we show that conditional deletion of Srsf3 in fully grown germinal vesicle oocytes substantially compromises the capacity of germinal vesicle breakdown (GVBD), and consequently entry into meiosis...
2018: Cell Discovery
Yuan Liu, Zhen Qi, Xintong Li, Yanan Du, Ye-Guang Chen
No abstract text is available yet for this article.
2018: Cell Discovery
Liang Zhang, Xiaoyi Li, Dekuan Li, Yuna Sun, Ying Li, Qin Luo, Zhibin Liu, Jianmei Wang, Xufeng Li, Hong Zhang, Zhiyong Lou, Yi Yang
The function of abscisic acid (ABA) is mediated by its receptors termed RCARs/PYR1/PYLs. Modulation of ABA signaling is vital for plant growth and development. The RCAR-PP2C-SnRK2 regulatory modules have been defined as the core components in ABA signaling. However, it is still not clear whether and how the ABA receptors could be modified at the initial post-translational stage to fine-tune ABA transduction pathway. Here we identify and characterize the putative receptor-like cytoplasmic kinase (RLCK) in Arabidopsis named CARK1, which interacts with RCAR3 (PYL8) and RCAR11 (PYR1) in the manner of phosphorylation...
2018: Cell Discovery
Chih-Chao Hsu, Dan Zhao, Jiejun Shi, Danni Peng, Haipeng Guan, Yuanyuan Li, Yaling Huang, Hong Wen, Wei Li, Haitao Li, Xiaobing Shi
The histone variant H2A.Z is essential for maintaining embryonic stem cell (ESC) identity in part by keeping developmental genes in a poised bivalent state. However, how H2A.Z is deposited into the bivalent domains remains unknown. In mammals, two chromatin remodeling complexes, Tip60/p400 and SRCAP, exchange the canonical histone H2A for H2A.Z in the chromatin. Here we show that Glioma Amplified Sequence 41 (Gas41), a shared subunit of the two H2A.Z-depositing complexes, functions as a reader of histone lysine acetylation and recruits Tip60/p400 and SRCAP to deposit H2A...
2018: Cell Discovery
Qijie Li, Liangbin Lin, Yanli Tong, Yantong Liu, Jun Mou, Xiaodong Wang, Xiuxuan Wang, Yanqiu Gong, Yi Zhao, Yi Liu, Bo Zhong, Lunzhi Dai, Yu-Quan Wei, Huiyuan Zhang, Hongbo Hu
[This corrects the article DOI: 10.1038/s41421-018-0010-9.].
2018: Cell Discovery
Shu Yang, Miao Xu, Emily M Lee, Kirill Gorshkov, Sergey A Shiryaev, Shihua He, Wei Sun, Yu-Shan Cheng, Xin Hu, Anil Mathew Tharappel, Billy Lu, Antonella Pinto, Chen Farhy, Chun-Teng Huang, Zirui Zhang, Wenjun Zhu, Yuying Wu, Yi Zhou, Guang Song, Heng Zhu, Khalida Shamim, Carles Martínez-Romero, Adolfo García-Sastre, Richard A Preston, Dushyantha T Jayaweera, Ruili Huang, Wenwei Huang, Menghang Xia, Anton Simeonov, Guoli Ming, Xiangguo Qiu, Alexey V Terskikh, Hengli Tang, Hongjun Song, Wei Zheng
The re-emergence of Zika virus (ZIKV) and Ebola virus (EBOV) poses serious and continued threats to the global public health. Effective therapeutics for these maladies is an unmet need. Here, we show that emetine, an anti-protozoal agent, potently inhibits ZIKV and EBOV infection with a low nanomolar half maximal inhibitory concentration (IC50 ) in vitro and potent activity in vivo. Two mechanisms of action for emetine are identified: the inhibition of ZIKV NS5 polymerase activity and disruption of lysosomal function...
2018: Cell Discovery
Demeng Sun, You Yu, Xiaobin Xue, Man Pan, Ming Wen, Siyu Li, Qian Qu, Xiaorun Li, Longhua Zhang, Xueming Li, Lei Liu, Maojun Yang, Changlin Tian
Acid-sensing ion channels (ASICs) are neuronal voltage-independent Na+ channels that are activated by extracellular acidification. ASICs play essential roles in a wide range of physiological processes, including sodium homeostasis, synaptic plasticity, neurodegeneration, and sensory transduction. Mambalgins, a family of three-finger toxins isolated from black mamba venom, specifically inhibit ASICs to exert strong analgesic effects in vivo, thus are thought to have potential therapeutic values against pain...
2018: Cell Discovery
Huawei Wang, John Lapek, Ken Fujimura, Jan Strnadel, Bei Liu, David J Gonzalez, Wei Zhang, Felicia Watson, Vicky Yu, Chao Liu, Carina Muccilo Melo, Yury I Miller, Kathryn C Elliott, David A Cheresh, Richard L Klemke
PEAK1 is a newly described tyrosine kinase and scaffold protein that transmits integrin-mediated extracellular matrix (ECM) signals to facilitate cell movement and growth. While aberrant expression of PEAK1 has been linked to cancer progression, its normal physiological role in vertebrate biology is not known. Here we provide evidence that PEAK1 plays a central role in orchestrating new vessel formation in vertebrates. Deletion of the PEAK1 gene in zebrafish, mice, and human endothelial cells (ECs) induced severe defects in new blood vessel formation due to deficiencies in EC proliferation, survival, and migration...
2018: Cell Discovery
Pengfei Kong, Maik J Lehmann, J Bernd Helms, Jos F Brouwers, Nishith Gupta
Successful inter-host transmission of most apicomplexan parasites requires the formation of infective sporozoites within the oocysts. Unlike all other infective stages that are strictly intracellular and depend on host resources, the sporozoite stage develops outside the host cells, but little is known about its self-governing metabolism. This study deployed Eimeria falciformis , a parasite infecting the mouse as its natural host, to investigate the process of phospholipid biogenesis in sporozoites. Lipidomic analyses demonstrated the occurrence of prototypical phospholipids along with abundant expression of at least two exclusive lipids, phosphatidylthreonine (PtdThr) and inositol phosphorylceramide with a phytosphingosine backbone, in sporozoites...
2018: Cell Discovery
Zhenglong Guo, Renwei Jing, Quan Rao, Ludi Zhang, Yimeng Gao, Fengyong Liu, Xin Wang, Lijian Hui, HaiFang Yin
Common marmoset ( Callithrix jacchus ) is emerging as a clinically relevant nonhuman primate model for various diseases, but is hindered by the availability of marmoset cell lines, which are critical for understanding the disease pathogenesis and drug/toxicological screening prior to animal testing. Here we describe the generation of immortalized marmoset hepatic progenitor cells (MHPCs) by lentivirus-mediated transfer of the simian virus 40 large T antigen gene in fetal liver polygonal cells. MHPCs proliferate indefinitely in vitro without chromosomal alteration and telomere shortening...
2018: Cell Discovery
Jingyi Yang, Yue Zhao, Peng Li, Yi Yang, Ejuan Zhang, Maohua Zhong, Yaoming Li, Dihan Zhou, Yuan Cao, Mengji Lu, Feng Shao, Huimin Yan
The NLR apoptosis inhibitory proteins (NAIPs) function as specific cytosolic receptors for bacterial ligands to form the NAIP-NLRC4 inflammasome for anti-bacterial defenses. In mice, NAIP5/6 and NAIP2 recognize bacteria flagellin and the rod protein of the type III secretion system (T3SS), respectively. However, molecular mechanism for specific ligand pattern-recognition by the NAIPs is largely unknown. Here, through extensive domain swapping and truncation analyses, three structural domains, the pre-BIR, BIR1, and HD1, in NAIP2 and NAIP5 are identified, that are important for specific recognition of their respective ligand(s)...
2018: Cell Discovery
Janet L Manias Rothberg, Harinad B Maganti, Hani Jrade, Christopher J Porter, Gareth A Palidwor, Christopher Cafariello, Hannah L Battaion, Safwat T Khan, Theodore J Perkins, Robert F Paulson, Caryn Y Ito, William L Stanford
Polycomb repressive complex 2 (PRC2) accessory proteins play substoichiometric, tissue-specific roles to recruit PRC2 to specific genomic loci or increase enzymatic activity, while PRC2 core proteins are required for complex stability and global levels of trimethylation of histone 3 at lysine 27 (H3K27me3). Here, we demonstrate a role for the classical PRC2 accessory protein Mtf2/Pcl2 in the hematopoietic system that is more akin to that of a core PRC2 protein. Mtf2 -/- erythroid progenitors demonstrate markedly decreased core PRC2 protein levels and a global loss of H3K27me3 at promoter-proximal regions...
2018: Cell Discovery
Shi-Yuan Li, Qiu-Xiang Cheng, Jing-Man Wang, Xiao-Yan Li, Zi-Long Zhang, Song Gao, Rui-Bing Cao, Guo-Ping Zhao, Jin Wang
No abstract text is available yet for this article.
2018: Cell Discovery
Yunlong Huang, Yuju Li, Hainan Zhang, Runze Zhao, Ran Jing, Yinghua Xu, Miao He, Justin Peer, Yeong C Kim, Jiangtao Luo, Zenghan Tong, Jialin Zheng
Zika virus (ZIKV) is a neurotrophic flavivirus that is capable of infecting humans, leading to brain abnormalities during fetal development. The ZIKV infectivity in neural target cells remains poorly understood. Here, we found that ZIKV specifically infected glial fibrillary acidic protein- and S100B-positive primary human astrocytes derived from fetal brains. In contrast, neuron-specific Class III β-tubulin (TuJ1)-positive neurons in the astrocyte cultures and SOX2-positive neural progenitor cells derived from the fetal brains were less susceptible to ZIKV infection compared with astrocytes...
2018: Cell Discovery
Guanqun Wang, Meijie Du, Jianbin Wang, Ting F Zhu
No abstract text is available yet for this article.
2018: Cell Discovery
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