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Veterinary World

Mamta Rani, Deepti Narang, Dinesh Kumar, Mudit Chandra, Sikh Tejinder Singh, G Filia
Background and Aim: Johne's disease is chronic granulomatous enteritis which affects ruminants. There are many diagnostic approaches for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) of which molecular detection methods using various elements are less time consuming and more accurate. The present study was conducted using ISMap 02 element for nested polymerase chain reaction (nPCR) based detection of MAP in fecal samples. The aim was to test the sensitivity and specificity of the ISMap 02 element and also to use this element for the detection of MAP in fecal samples of cattle and buffaloes...
March 2018: Veterinary World
Mohamed Nadir Meguini, Souad Righi, Fayçal Zeroual, Khelaf Saidani, Ahmed Benakhla
Background and Aim: Lice are permanent ectoparasites, extremely specific to their hosts. Their great importance in veterinary medicine remain significant, they can cause their direct pathogenic actions like irritability, dermatitis, anemia, decreased weight gain, and milk production. The purpose of this work was to made the first time an inventory of mammalian lice in North-eastern Algeria. Materials and Methods: Our survey of lice infestation was conducted on several animal species from five provinces of North-eastern Algeria...
March 2018: Veterinary World
Sreepriya Prakasan, Parmanand Prabhakar, Manjusha Lekshmi, Sanath Kumar, Binaya Bhusan Nayak
Background and Aim: Shiga toxin-producing Escherichia coli (STEC) are important pathogens of global significance. STEC are responsible for numerous food-borne outbreaks worldwide and their presence in food is a potential health hazard. The objective of the present study was to determine the incidence of STEC in fresh seafood in Mumbai, India, and to characterize STEC with respect to their virulence determinants. Materials and Methods: A total of 368 E. coli were isolated from 39 fresh seafood samples (18 finfish and 21 shellfish) using culture-based methods...
March 2018: Veterinary World
Merradi Manel, Heleili Nouzha, Mekari Rim, Mekkaoui Imane, Aouachria Sana, Oucheriah Yasmine, Ayachi Ammar
Aim: This work aimed to evaluate the biological activity of the leaf extracts of Juniperus thurifera L., which is an Algerian endemic tree that belongs to the family of Cupressaceae. Materials and Methods: The plant leaves were extracted in solvents of increasing polarity to obtain different extracts such as methanol, petroleum ether, chloroform, ethyl acetate, and aqueous extracts (MeE, PEE, ChlE, EtAE, and AqE). The antioxidant activity of four extracts (MeE, ChlE, EtAE, and AqE) was assessed by trapping test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical...
March 2018: Veterinary World
Ngadino, Setiawan, Koerniasari, Ernawati, S A Sudjarwo
Aim: The aim of this study was to evaluate the antimycobacterial activity of the Curcuma xanthorrhiza ethanolic extract in vitro . Materials and Methods: Ethanolic extract of C. xanthorrhiza was set by maceration method. The broth microdilution and disc diffusion method were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), respectively, of C. xanthorrhiza ethanol extract on strain Mycobacterium tuberculosis H37Rv...
March 2018: Veterinary World
Rabehi Sabrina, Hamdi Taha Mossadak, Mamache Bakir, Meghezzi Asma, Boushaba Khaoula
Aim: The aim of this study was to detect Brucella spp. DNA in milk samples collected from seronegative cows using the real-time polymerase chain reaction (PCR) assay for diagnosis of brucellosis in seronegative dairy cows to prevent transmission of disease to humans and to reduce economic losses in animal production. Materials and Methods: In this study, 65 milk samples were investigated for the detection of Brucella spp. The detection of the IS711 gene in all samples was done by real-time PCR assay by comparative cycle threshold method...
March 2018: Veterinary World
Abeer Mostafa Abdalhamed, Gamil Sayed Gamil Zeedan, Hala Abdoula Ahmed Abou Zeina
Aim: The present work aims to isolate and identify bacteria that cause mastitis in small ruminants and evaluates the antibacterial activity of some antibiotics, honey, essential oils, and plant extracts. Materials and Methods: A total of 289 milk samples were collected from udder secretions of sheep (n=189) and goat (n=100) from El-Fayoum, Beni-Suef, and Giza governorates. Screening subclinical mastitis (SCM) was done using California Mastitis Test (CMT); identification of the isolates was achieved using Gram's staining, hemolytic pattern, colony morphology, and biochemical tests using Analytical Profile Index...
March 2018: Veterinary World
L Babita Devi, Durlav Prasad Bora, S K Das, R K Sharma, S Mukherjee, R A Hazarika
Aim: The present study was conducted to detect and identify the virulence genes in Pasteurella multocida isolates of porcine origin from Assam. Materials and Methods: A total of 21 porcine P. multocida isolates were subjected to capsular typing and detection of virulence-associated genes ( pfhA, tbpA, hgbB, toxA, oma87, ompH , and nanB ) using various polymerase chain reaction (PCR) methods reported elsewhere. Further, pathogenicity of the porcine isolates of P...
March 2018: Veterinary World
Intan Nirwana, Devi Rianti, R Helal Soekartono, Rr Dwi Listyorini, Desi Putri Basuki
Background: Enterococcus faecalis is one of the bacteria that commonly found in root canal and pulp infection after root canal treatment. Sodium hypochlorite is the most widely used root canal irrigation, but it has toxic properties if exposed to periradicular tissues. It is necessary to develop an alternative for root canal irrigation. Fig leaf ( Ficus carica Linn.) extract contains active substances such as flavonoid, tannin, and terpenoid which have been known for their antibacterial potency...
March 2018: Veterinary World
Masoud Soosaraei, Mousa Motavalli Haghi, Fariborz Etemadifar, Mahdi Fakhar, Saeed Hosseini Teshnizi, Hajar Ziaei Hezarjaribi, Shabnam Asfaram
Aim: Theileriosis is a protozoal disease caused by Theileria spp. mostly in warm-blooded vertebrates worldwide. It is one of the common tick-borne diseases among domestic animals in tropical and sub-tropical regions, which have a variety of unlikable effects on health economy and animal welfare. In the present study, the prevalence of theileriosis among domestic farm animals in Iran was systematically evaluated. Methods: To identify the related papers, 10 English and Persian databases, including PubMed, Science Direct, Scopus, Web of Science, Medical Subject Headings, Google Scholar, Magiran, Barakatns (formerly Iranmedex), Elm net, and Scientific Information Database, were appraised for articles published throughout 1999-2017...
March 2018: Veterinary World
Diyana Tahir, M Shariff, Fadhil Syukri, F M Yusoff
Background and Aim: Brown-marbled grouper Epinephelus fuscoguttatus is a premium marine food fish with high demand in Asia. In fish, stress due to environmental changes such as fluctuations in the salinity can result in increased cortisol level. Stress in fish increases susceptibility to diseases ultimately resulting in death. Therefore, the aim of this study was to investigate the salinity tolerance of E. fuscoguttatus and their survival in lower salinities. Materials and Methods: In this study, grouper juveniles (92...
March 2018: Veterinary World
Wiwik Misaco Yuniarti, Hardany Primarizky, Bambang Sektiari Lukiswanto
Aim: This research aimed to evaluate the effects of pomegranate extract standardized to 40% ellagic acid on the incised wound in albino rats. Materials and Methods: Fifty albino rats were divided into 10 treatment groups. The five groups were sacrificed on the 8th day, while the others were sacrificed on the 15th day. Two groups of albino rats with incised wound were not treated at all (P0), the other two groups of albino rats with incised wound were treated with Betadine® (P1) ointment, and the rest of the groups were treated with pomegranate extract standardized to 40% ellagic acid with a concentration of 2...
March 2018: Veterinary World
Neeraj Shrivastava, Varsha Sharma, Arpita Shrivastav, Anju Nayak, Ajay Kumar Rai
Aim: The study aimed to investigate the Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus in bovine milk due to its public health significance. Materials and Methods: A total of 400 milk samples of bovines taken from different dairy farms and outlets of Jabalpur were screened for the S. aureus and methicillin-resistant S. aureus (MRSA). The strains were tested for the PVL gene and antimicrobial sensitivity toward 10 different classes of antimicrobial agents...
March 2018: Veterinary World
Fayez A Bakry, Marwa Th Atwa, Marwa M Attia
Aim: The present study aims to focus on the role of common marine snails ( Littorina littorea) as a vector for some trematode parasites. Materials and Methods: A total of 327 marine water L. littorea snails were collected during the summer of 2016 from a Qarun lake in the EL-Fayoum Governorate, Egypt. The snails were investigated for infection by trematode parthenitae through induction of cercarial shedding by exposure to light and crushing the snails. The species were stored in Search Laboratory of Zoology Department, Faculty of Science, Fayoum University...
March 2018: Veterinary World
Diah Savitri Ernawati, Ade Puspa
Aim: The aim of this study was to determine the effect of Apis mellifera propolis extract gel on vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) expression in the traumatic ulcers of rats afflicted with diabetes mellitus (DM). Materials and Methods: The study was conducted on 24 male Wistar rats ( Rattus norvegicus ) induced with DM by injecting 50 mg/kg of Streptozotocin, intraperitoneally, and a traumatic ulcer on their lower lip mucosa...
March 2018: Veterinary World
S S Patil, K P Suresh, S Saha, A Prajapati, D Hemadri, P Roy
Aim: The aim of the study was to determine the overall prevalence of classical swine fever (CSF) in pigs in India, through a systematic review and meta-analysis of published data. Materials and Methods: Consortium for e-Resources in Agriculture, India, Google Scholar, PubMed, annual reports of All India Coordinated Research Project on Animal Disease Monitoring and Surveillance, and All India Animal Disease database of NIVEDI (NADRES) were used for searching and retrieval of CSF prevalence data (seroprevalence, virus antigen, and virus nucleic acid detection) in India using a search strategy combining keywords and related database-specific subject terms from January 2011 to December 2015 in English only...
March 2018: Veterinary World
Keshaw Tiwari, Camille Coomansingh Springer, Alfred Chikweto, Josephine Tang, Yvette Sepulveda, Amanda Leigh Smith, Nia Rametta, Ravindra Nath Sharma
Aim: Giardia is a serious zoonotic parasite, which causes diarrheal disease in humans and animals including rodents. The purpose of this study was to estimate the prevalence of Giardia spp. in brown rats ( Rattus norvegicus ) in Grenada. Materials and Methods: Intestinal contents from 99 and serum samples from 169 brown rats ( R. norvegicus ) from Grenada were collected. These samples were examined for the Giardia coproantigens using Cryptosporidium / Giardia Quik Chek assay (Tech lab® Inc...
March 2018: Veterinary World
Moulay Abdelmonaim El Hidan, Oulaid Touloun, Abdellah Bouazza, Mehdi Ait Laaradia, Ali Boumezzough
Aim: The objective of this study was to establish environmental factors related to scorpion species occurrence and their current potential geographic distributions in Morocco, to produce a current envenomation risk map and also to assess the human population at risk of envenomation. Materials and Methods: In this study, 71 georeferenced points for all scorpion species and nine environmental indicators were used to generate species distribution models in Maxent (maximum entropy modeling of species geographic distributions) version 3...
March 2018: Veterinary World
M Khezri, M Moharrami, H Modirrousta, M Torkaman, B Rokhzad, H Khanbabaie
Aim: Paenibacillus larvae subsp. larvae is the etiological agent of American foulbrood (AFB), the most virulent bacterial disease of honey bee brood worldwide. In many countries, AFB is a notifiable disease since it is highly contagious, in most cases incurable, and able to kill affected colonies. The aim of this study was to determine the prevalence of P. larvae subsp . larvae in Kurdistan province apiaries by polymerase chain reaction (PCR) technique. Materials and Methods: A total of 100 samples were randomly purchased from apiaries in Kurdistan, Iran...
March 2018: Veterinary World
Vikash Kumar, Jancy Gupta
Aim: The aim of the study was to assess the antibiotic use in dairy animals and to trace its usage pattern among the small, medium, and large dairy farmers in Eastern Haryana region of India. Materials and Methods: Karnal and Kurukshetra districts from Eastern region of Haryana state were purposively selected, and four villages from each district were selected randomly. From each village, 21 farmers were selected using stratified random sampling by categorizing into small, medium, and large farmers constituting a total of 168 farmers as respondents...
March 2018: Veterinary World
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