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Veterinary World

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https://www.readbyqxmd.com/read/28717327/virulence-gene-profiles-of-arcobacter-species-isolated-from-animals-foods-of-animal-origin-and-humans-in-andhra-pradesh-india
#1
M Soma Sekhar, S R Tumati, B K Chinnam, V S Kothapalli, N Mohammad Sharif
AIM: This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin. MATERIALS AND METHODS: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349)...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717326/honey-can-repairing-damage-of-liver-tissue-due-to-protein-energy-malnutrition-through-induction-of-endogenous-stem-cells
#2
R Heru Prasetyo, Eka Pramyrtha Hestianah
AIM: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells. MATERIALS AND METHODS: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717325/tetracycline-resistance-phenotypes-and-genotypes-of-coagulase-negative-staphylococcal-isolates-from-bubaline-mastitis-in-egypt
#3
K A Abd El-Razik, A A Arafa, R H Hedia, E S Ibrahim
AIM: This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt. MATERIALS AND METHODS: A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotypic and molecular methods (polymerase chain reaction [PCR]). CNS isolates were tested for tetracycline resistance using routine methods and multiplex PCR targeting tetracycline (tet) resistance genes followed by sequencing of positive PCR products and phylogenetic analysis...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717324/investigation-of-avian-influenza-infection-in-wild-birds-in-ismailia-and-damietta-cities-egypt
#4
Hanaa Mohamed Fadel, Rabab Afifi
AIM: This study was carried out to monitor avian influenza (AI) infection in wild birds in Egypt. MATERIALS AND METHODS: A total of 135 wild birds were examined for the presence of H5, H7, and H9 hemagglutination inhibition antibodies. Organs and swab samples of 75 birds were screened by multiplex real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) to detect AI subtypes H5, H7, and H9 matrix genes. RESULTS: The highest seropositive result was recorded in cattle egrets (90...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717323/epidemiological-study-of-tick-infestation-in-buffalo-of-various-regions-of-district-khairpur-pakistan
#5
Farzana Abbasi, Imtiaz Hussain Raja Abbasi, Tahmeena Fakhur Nissa, Zohaib Ahmed Bhutto, Muhammed Asif Arain, Rab Nawaz Soomro, Farman Ali Siyal, Sarfraz Ali Fazlani
AIM: The aim of this study was to determine the epidemiological infestation and identification of Ixodidae and Argasidae ticks species in buffalo of different parts of district Khairpur, Pakistan. MATERIALS AND METHODS: A total of 720 Water buffaloes from three tehsils (subdivisions) were selected randomly and examined from organized and unorganized dairy farms for tick infestation in district Khairpur, Pakistan. This epidemiological survey was conducted during April to September 2015...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717322/th1-th2-balance-and-humoral-immune-response-to-potential-antigens-as-early-diagnostic-method-of-equine-strongylus-nematode-infection
#6
Faten A M Abo-Aziza, Seham H M Hendawy, Amira H El Namaky, Heba M Ashry
AIM: The aim of this study was to investigate the early diagnosis of strongyle infection based on early changes in Th1 and Th2 cytokines beside the diagnostic accuracy values and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting profiles using prepared strongyles antigens. MATERIALS AND METHODS: A total of 73 donkeys had a mean age of 4-32 years old were parasitologically examined for strongyle infection. The early changes in Th1 and Th2 cytokines were determined, and the diagnostic accuracy values and SDS-PAGE and western blotting profiles were performed using prepared strongyles antigens; crude somatic Strongylusvulgaris (CSS), excretory-secretory S...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717321/effect-of-different-concentrations-of-soybean-lecithin-and-virgin-coconut-oil-in-tris-based-extender-on-the-quality-of-chilled-and-frozen-thawed-bull-semen
#7
A A Tarig, H Wahid, Y Rosnina, N Yimer, Y M Goh, F H Baiee, A M Khumran, H Salman, M A Assi, M Ebrahimi
AIM: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS: A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%)...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717320/coxiellosis-in-domestic-livestock-of-puducherry-and-tamil-nadu-detection-of-coxiella-burnetii-dna-by-polymerase-chain-reaction-in-slaughtered-ruminants
#8
Jothimani Pradeep, Selvaraj Stephen, Pratheesh Pooja, Anbalagan Akshayavardhini, Balakrishnan Sangeetha, Prabakar Xavier Antony
BACKGROUND AND AIM: In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). In this preliminary study, we have proceeded to look for C. burnetii DNA in those antibody positive specimens employing an imported commercial C. burnetii polymerase chain reaction (PCR) kit...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717319/changes-in-histopathology-and-cytokeratin-ae1-ae3-expression-in-skin-graft-with-different-time-on-indonesian-local-cats
#9
Erwin, Etriwati, Gunanti, Ekowati Handharyani, Deni Noviana
AIM: A good skin graft histopathology is followed by formation of hair follicle, sweat gland, sebaceous gland, blood vessel, lightly dense connective tissue, epidermis, and dermis layer. This research aimed to observe histopathology feature and cytokeratin AE1/AE3 expression on cat skin post skin grafting within a different period of time. MATERIALS AND METHODS: Nine male Indonesian local cats aged 1-2 years old weighing 3-4 kg were separated into three groups. First surgery created defect wound of 2 cm × 2 cm in size to whole groups...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717318/hemogram-responses-in-goats-toward-challenged-with-corynebacterium-pseudotuberculosis-and-its-immunogen-mycolic-acids
#10
Mohammed Naji Odhah, Faez Firdaus Jesse Abdullah, Abd Wahid Haron, Mohd Azmi Mohd Lila, Mohd Zamri-Saad, Zaid Khuder, Idris Umar Hambali, Muhammed Umar, Wessam Monther Saleh
AIM: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. MATERIALS AND METHODS: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×10(9)) colony forming unit of active C...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717317/effect-of-season-on-physiological-biochemical-hormonal-and-oxidative-stress-parameters-of-indigenous-sheep
#11
Sawankumar D Rathwa, A A Vasava, M M Pathan, S P Madhira, Y G Patel, A M Pande
AIM: This study was conducted to evaluate the impact of summer and winter season on physiological, biochemical, hormonal, and antioxidant parameters in Indigenous sheep. MATERIALS AND METHODS: The research was carried out during summer and winter season. 8 adult apparently healthy female sheep (aged 2-4 years) of similar physiological status were selected. Daily ambient temperature and relative humidity were recorded to calculate the temperature-humidity index (THI)...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717316/induced-pluripotent-stem-cell-a-headway-in-reprogramming-with-promising-approach-in-regenerative-biology
#12
REVIEW
N Rawat, M K Singh
Since the embryonic stem cells have knocked the doorsteps, they have proved themselves in the field of science, research, and medicines, but the hovered restrictions confine their application in human welfare. Alternate approaches used to reprogram the cells to the pluripotent state were not up to par, but the innovation of induced pluripotent stem cells (iPSCs) paved a new hope for the researchers. Soon after the discovery, iPSCs technology is undergoing renaissance day by day, i.e., from the use of genetic material to recombinant proteins and now only chemicals are employed to convert somatic cells to iPSCs...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717315/seroprevalence-of-antibodies-to-astrovirus-in-chickens-in-grenada-west-indies
#13
Ravindra Nath Sharma, Romane Dufayet, Thomas Maufras, Kathryn O' Connell, Keshaw Tiwari
AIM: Chicken astroviruses (CAstV) are known to cause mild gastroenteritis, growth depression, and even mortality in poultry, especially in chickens, turkeys, and ducks. To the best our knowledge, there is no published information on CAstV in Grenada. This study was conducted to determine the prevalence of astrovirus in chickens in Grenada. MATERIALS AND METHODS: Blood samples from 366 indigenous chickens and 92 commercial chicken layers were collected from all parishes of the island and tested for antibodies against CAstV using commercial enzyme-linked immunosorbent assay...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717314/enhancing-the-growth-performance-of-replacement-female-breeder-goats-through-modification-of-feeding-program
#14
A A A Ghani, M S Shahudin, M Zamri-Saad, A B Zuki, H Wahid, A Kasim, M S Salisi, Hasliza Abu Hassim
AIM: The study was conducted at a smallholder goat farm located in Labu, Negeri Sembilan, Malaysia. The objective of this study was to evaluate the effect of proper feeding program on growth performances of replacement breeder goats. MATERIALS AND METHODS: A total of 30 healthy female boer cross goats at the age of 4 months old with average initial live body weight (BW) of 20.05±0.5 kg were used for on-farm feeding trial to evaluate the growth performance as preparation for breeding purposes...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717313/study-of-morphology-chemical-and-amino-acid-composition-of-red-deer-meat
#15
Eleonora Okuskhanova, Bahytkul Assenova, Maksim Rebezov, Kumarbek Amirkhanov, Zhanibek Yessimbekov, Farida Smolnikova, Almagul Nurgazezova, Gulnur Nurymkhan, Marilyne Stuart
AIM: The aim of this study was to evaluate red deer (maral) meat quality based on chemical composition, pH, water-binding capacity (WBC), and amino acid content. MATERIALS AND METHODS: Maral meat surface morphology measurements were obtained by scanning electron microscopy. Active acidity (pH) was determined by potentiometry. Samples were analyzed for WBC by exudation of moisture to a filter paper by the application of pressure. Chemical composition (moisture, protein, fat, and ash fractions) was obtained by drying at 150°C and by extraction, using ethylic ether, and ashing at 500-600°C...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717312/effect-of-high-and-low-roughage-total-mixed-ration-diets-on-rumen-metabolites-and-enzymatic-profiles-in-crossbred-cattle-and-buffaloes
#16
S K Sinha, V B Chaturvedi, Putan Singh, L C Chaudhary, Mayukh Ghosh, Swati Shivani
AIM: A comparative study was conducted on crossbred cattle and buffaloes to investigate the effect of feeding high and low roughage total mixed ration (TMR) diets on rumen metabolites and enzymatic profiles. MATERIALS AND METHODS: Three rumen-fistulated crossbred cattle and buffalo were randomly assigned as per 3×3 switch over design for 21-days. Three TMR diets consisting of concentrate mixture, wheat straw and green maize fodder in the ratios of (T1) 60:20:20, (T2) 40:30:30, and (T3) 20:40:40, respectively, were fed to the animals ad libitum...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717311/using-real-time-polymerase-chain-reaction-as-an-alternative-rapid-method-for-enumeration-of-colony-count-in-live-brucella-vaccines
#17
Waleed S Shell, Mahmoud L Sayed, A A Samy, Ghada Mohamed Al-Sadek, Gina Mohamed Mohamed Abd El-Hamid, Abdel Hakam M Ali
AIM: Brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. It has economic, public health, and bio-risk importance. Control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. There are three types of animal Brucella live vaccines - Brucella melitensis Rev-1 vaccine, Brucella abortus S19, and B. abortus RB51. Evaluation of these vaccines depends mainly on enumeration of Brucella viable count...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717310/cytological-endometritis-and-its-agreement-with-ultrasound-examination-in-postpartum-beef-cows
#18
N Salah, N Yimer
BACKGROUND: Endometritis, which is one of the most common diseases in dairy cows postpartum, causes severe economic losses, including increased open days, calving intervals, and numbers of services to achieve conception. AIM: This study aimed to evaluate the ultrasound method and its agreement with the endometrium cytology method, which is used to diagnose cytological endometritis in beef cows. Moreover, we determined which method has higher sensitivity and specificity at 4 and 5 weeks postpartum...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717309/molecular-characterization-of-antibiotic-resistant-staphylococcus-aureus-from-livestock-bovine-and-swine
#19
Asima Zehra, Randhir Singh, Simranpreet Kaur, J P S Gill
AIM: The aim of this study was to figure the prevalence, phenotypic and genotypic antibiotic resistance (AR) pattern of Staphylococcus aureus isolated from bovine and swine nares. MATERIALS AND METHODS: Colonies with typical morphology on Baird-Parker agar supplemented with egg-yolk tellurite emulsion were selected and biochemically/genotypically identified as S. aureus. These strains were further subjected to epsilometer test for their sensitivity to various clinically important antibiotics and antibiotic susceptibility testing for amoxicillin/clavulanic acid, and double-disk diffusion testing was performed by the standard disc diffusion method following CLSI guidelines...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717308/prevalence-of-methicillin-resistant-staphylococcus-aureus-skin-and-nasal-carriage-isolates-from-bovines-and-its-antibiogram
#20
Alok Kumar, Purushottam Kaushik, Anjay, Pankaj Kumar, Manoj Kumar
AIM: This study was conducted to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in cattle and buffalo and to study their antibiotic resistance pattern. MATERIALS AND METHODS: A total of 136 samples (skin and nasal swab) from cattle and buffalo were collected. MRSA was identified by conventional bacterial culture techniques which were further confirmed by amplification of S. aureus-specific 16S rRNA by polymerase chain reaction (PCR)...
June 2017: Veterinary World
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