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Molecular Biology Research Communications

Mahsa Salehi, Mohammadreza Sharifi
Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell growth, proliferation, differentiation, apoptosis and migration are regulated by lncRNAs. In various cancers, aberrant expression of lncRNAs has been reported...
June 2018: Molecular Biology Research Communications
Roya Zanganeh, Rashid Jamei, Fatemeh Rahmani
Heavy metals pollution is one of the key environmental problems. In this research, the effect of seed priming with salicylic acid and sodium hydrosulfide was investigated on methionine and arginine amino acids contents and some compounds derived from their metabolism as well as ZmACS6 and ZmSAMD transcripts levels in maize plants under lead stress. For this purpose, maize seeds were soaked in salicylic acid (0.5 mM) and sodium hydrosulfide (0.5 mM) for 12 hours and then exposed to lead (2.5 mM) for 9 days. The results showed that lead stress reduced nitric oxide content and shoot ZmACS6 and ZmSAMD transcript levels while increased glycine betaine, methionine, arginine and proline amino acids contents as well as root ZmACS6 and ZmSAMD transcript levels...
June 2018: Molecular Biology Research Communications
Hussain Mubbashir, Shahzad Munir, Rahim Kashif, Haider Bashir Nawaz, Basit Abdul, Khattak Baharullah
Dengue is a vector-borne disease caused by dengue virus. According to the recent report of CDC that one-third population of the world are at high risk with Dengue fever. The prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. Aedes mosquitoes was reported as the main cause of transmission of dengue virus. So the current study was planned to characterize the virus in Aedes mosquitoes collected from different area of Pakistan. In current investigation, Aedes mosquitoes and larvae were trapped under conducive conditions which are counted as 495 Aedes mosquitoes and 260 Aedes larvae...
June 2018: Molecular Biology Research Communications
Sandeep Saini, Chander Jyoti-Thakur, Varinder Kumar, Akshay Suhag, Niharika Jakhar
Interleukin-4 (IL-4) is a multifunctional cytokine that plays a critical role in apoptosis, differentiation and proliferation. The intensity of IL4 response depends upon binding to its receptor, IL-4R. The therapeutic efficiency of interleukins can be increased by generating structural mutants having greater stability. In the present work, attempts were made to increase the stability of human IL-4 using in-silico site directed mutagenesis. Different orthologous sequences of IL4 from Pan troglodytes , Aotusnigriceps , Macacamulatta , Papiohamadryas , Chlorocebusaethiops , Vicugnapacos , Susscrofa and Homo sapiens were aligned using Clustal Omega that revealed the conserved and non-conserved positions...
June 2018: Molecular Biology Research Communications
Habib Ghaznavi, Khalil Mahmoodi, Mohammad Soleiman Soltanpour
Coronary artery disease (CAD) is a common health problem in Iranian population. ATP binding cassette transporter A1 (ABCA1) plays central role in the efflux of the cholesterol from peripheral tissues back to liver. Inactivation of ABCA1 by epigenetic change such as DNA methylation may contribute to the development of CAD. The present study investigated the association between promoter DNA methylation status of ABCA1 with the development and severity of CAD. Our study population consisted of 110 angiographically documented CAD patients and 110 controls...
June 2018: Molecular Biology Research Communications
Olukanni A Titilayo, Amoo O Samuel, Olukanni O David, Taiwo I Adewunmi
Genetically, every individual is unique; this may stem from inheritance, geographical locations, and/or environmental interactions. This study examined the possibility of developing a cheap and easy-to-use marker that can distinguish among the three ethnic groups in Nigeria using RAPD-PCR. Five RAPD primers, OPA1-3 and OPC1-2, were randomly selected and used to amplify DNA samples isolated from blood of eighteen human subjects representing the three major ethnic groups in Nigeria (six subjects each). Genomic DNAs were extracted using DNA isolation kit, RAPD-PCR amplification was performed and gel electrophoresis was done...
June 2018: Molecular Biology Research Communications
Aminallah Tahmasebi, Alireza Afsharifar, Ahmad Heydari, Mohammad Mehrabadi
The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysis of 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K coding regions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotypes were detected with haplotype diversity ranging from 0.8 to 1.0 and nucleotide diversity from 7.58 to 13.62. The positions 33 and 30 indicated the highly positive and negative selection (with the highest and the lowest dN-dS values), respectively...
March 2018: Molecular Biology Research Communications
Samira Ebrahimi, Hassan Mohabatkar
Streptococcal bacteria are among dangerous human pathogens with major prevalence worldwide. A good vaccine against streptococcal bacteria should have epitopes that confer protection from infection by different streptococcal bacteria types. we aimed was to recognize the most immunogenic and conserved epitopes of streptococcal bacteria, which could be a potential candidate for vaccine development. Nineteen different M proteins of different streptococcal bacteria were chosen and analyzed. Nine-mer epitopes able to simulate both cells mediate and humoral immunity were predicted...
March 2018: Molecular Biology Research Communications
Mohammadreza Sharifi, Mahdi Fasihi-Ramandi, Abdolkarim Sheikhi, Abbas Moridnia, Maryam Saneipour
MicroRNAs (miRNAs) involved in regulation of the genes. The CCAAT/enhancer-binding protein-α ( CEBPα ) is a crucial transcription factor for normal hematopoiesis and cell cycle that frequently disrupted in human acute myeloid leukemia (AML). The miR-182 up-regulation in several malignant diseases such as AML was reported, in the other hand bioinformatics analysis revealed CEBPα targeted by miR-182.miR-182-5p inhibition in human acute promyelocytic leukemia (APL) cell line was performed by using locked nucleic acid (LNA) and subsequently miR-182-5p and CEBPα expression, apoptosis, necrosis and cell proliferation were measured...
March 2018: Molecular Biology Research Communications
Abbas Seidi, Sina Mirzaahmadi, Khalil Mahmoodi, Mohammad Soleiman-Soltanpour
Coronary artery disease (CAD) is considered as a chronic inflammatory disease initiated from early childhood. Nuclear factor κB (NF κB) and κB1A (NF κB1A) are the key regulators of inflammatory responses. The NFKB1 -94ATTG ins/del and NFKB1A -826C/T polymorphisms may contribute to the development of CAD. The aim of the present study was to investigate the association of these polymorphisms with the risk of CAD. The study population included 120 patients with angiographically confirmed CAD and 100 matched controls...
March 2018: Molecular Biology Research Communications
Thoungamba Amom, Leimapokpam Tikendra, Hamidur Rahaman, Angamba Potshangbam, Potshangbam Nongdam
The classification of bamboos based on floral morphology and reproductive characters is very hard due to erratic flowering behavior and unusually long reproductive cycle. The application of reliable and effective DNA molecular markers is highly essential to address this problem. In the present investigation, inter-simple sequence repeats (ISSR) markers were employed to study phylogenetic relationship of 15 different bamboos of North-East India. The 10 ISSR primers generated 116 polymorphic loci or scorable bands with average of 11...
March 2018: Molecular Biology Research Communications
Mohammad Hashemi, Mansour Shakiba, Sara Sanaei, Ghazaleh Shahkar, Maryam Rezaei, Azizolla Mojahed, Gholamreza Bahari
Genetic factors are supposed to account for about 30-50% of the predisposition to cocaine and heroin addiction. This study aims at investigating the effect of rs2281285, rs2235749, rs910080 and 68bp VNTR polymorphisms of prodynorphin ( PDYN ) gene on heroin dependence risk in a sample of the southeast Iranian population. This case-control study was done on 216 heroin dependence subjects and 219 healthy subjects. Genomic DNA was extracted from peripheral blood cells using salting out method. Genotyping of PDYN polymorphisms were performed using polymerase chain reaction (PCR) or PCR-RFLP method...
March 2018: Molecular Biology Research Communications
Zahra Shakeran, Mehrnaz Keyhanfar, Mustafa Ghanadian
The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Datura metel was used as an advantageous method for production of scopolamine. The hairy roots are formed by Agrobacterium rhizogenes and have genetic stability, high growth rate and lateral branching...
December 2017: Molecular Biology Research Communications
Vida Eraghi, Abdollah Derakhshandeh, Arsalan Hosseini, Azar Motamedi-Boroojeni
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in ruminants and there has been a shift in the public health approach to MAP and human diseases like Crohn's disease. The prevention of infection by MAP in ruminants is thought to deter the high impact of economic losses in the level of dairy industry and possible spreading of this pathogen in dairy products. The present study was done to investigate the construction and expression of the soluble form of a novel fusion protein, consisting of Heparin-binding hemagglutinin (HBHA) and high antigenic region of Fibronectin Attachment Protein-P (FAP-P), in order to introduce as a Th1 inducer subunit vaccine against MAP...
December 2017: Molecular Biology Research Communications
Ali Safaie-Farahani, S Mohsen Taghavi
The transcript levels of six defense genes including pathogenesis-related gene 1 ( PR-1 ), pathogenesis-related gene 2 ( PR-2 ), pathogenesis-related gene 5 ( PR-5 ), lipoxygenase ( LOX ), phenylalanine ammonia-lyase ( PAL ) and catalase ( CAT ) were investigated in tomato plants inoculated with Xanthomonas axonopodis pv. phaseoli as a non-host pathogen and X. euvesicatoria as a host pathogen. Activation of all the genes was confirmed in both host and non-host treatments. Additionally, the results showed stronger expression of majority of the genes ( PR-1 , PR-2 , LOX , PAL and CAT ) in non-host treatment compared to host treatment at least at early hours after inoculation...
December 2017: Molecular Biology Research Communications
Sareh Yaripour, Hamid Reza Esmaeili, Ali Gholamhosseini, Mohammad Rezaei, Saber Sadeghi
Genetic structure of an endemic tooth-carp fish, Aphanius farsicus from four different water bodies in the Maharlu Lake basin was investigated by applying five microsatellite markers. All of the five examined microsatellite loci showed polymor-phism pattern. A total of four alleles were detected at five microsatellite loci, with an average of 2.8 to 3.5 alleles per locus. Average values of observed and expected heterozygosity were 0.95±0.09 and 0.64±0.02 respectively. None of the tests of linkage disequilibrium were significant between each pair of loci and no deviation from Hardy-Weinberg equilibrium were detected to test for heterozygote deficiency within populations...
December 2017: Molecular Biology Research Communications
María Inés Trucco, Claudio César Buratti
Taxonomically, Argentine mackerels were first considered as Scomber japonicus marplatensis and later as Scomber japonicus Houttuyn 1782, although, in the last years, different studies have suggested that South Atlantic mackerel species belongs to Scomber colias Gmelin 1789. These latter results, incorporated in the main fish databases (FishBase and Catalog of Fishes), promoted a phylogenetic study using cytochrome c oxidase I (COI) gene sequences taken from the Barcode of Life (FISH-BOL) database. Thus, 76 sequences of S...
September 2017: Molecular Biology Research Communications
Farveh Ehya, Hossein Abdul Tehrani, Masoud Garshasbi, Seyed Masood Nabavi
Many studies have investigated misregulation of miRNAs relevant to multiple sclerosis (MS) pathogenesis. Abnormal miRNAs can be used both as candidate biomarker for MS diagnosis and understanding the disease miRNA-mRNA regulatory network. In this comprehensive study, misregulated miRNAs related to MS were collected from existing literature, databases and via in silico prediction. A multi-staged data integration strategy (including the construction of miRNA-mRNA regulatory network and systematic data analysis) was conducted in order to investigate MS related miRNAs and their regulatory networks...
September 2017: Molecular Biology Research Communications
Mansour Shakiba, Mohammad Hashemi, Salah Mahdar, Zahra Rahbari, Gholamreza Bahari
The exact role of dopamine transporter gene (DAT1) in the pathogenesis of bipolar disorder type 1 (BD) is not understood. In the present study, we aimed to evaluate the possible association between 30, 40 and 63 bp variable number tandem repeat (VNTR) polymorphisms of DAT1 gene and the risk of type 1 (BD) in a sample of Iranian population. This case-control study was performed on 152 BD patients and 153 psychiatrically healthy subjects. Genotyping of the variant was done by polymerase chain reaction method...
September 2017: Molecular Biology Research Communications
Partha Sarathi Mohanty, Farah Naaz, Avi Kumar Bansal, Umesh Datta Gupta
The emergence of drug resistance in leprosy is a major hurdle in leprosy elimination programme. Although the problem of drug resistance is presently not acute, it is important that we collect data more systematically and monitor the trend carefully so that effective measures to combat this problem can be developed. The present study aimed at the explication of cross resistance of rifabutin and rifapentine to rifampicin which would be helpful to programme managers for implementing rifabutin or rifapentine in replace of rifampicin...
September 2017: Molecular Biology Research Communications
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