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Molecular Biology Research Communications

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https://www.readbyqxmd.com/read/28097172/transcript-levels-of-phytoene-desaturase-gene-in-dunaliella-salina-teod-as-affected-by-pbs-nanoparticles-and-light-intensity
#1
Hajar Zamani, Ali Moradshahi
Phytoene synthase (Psy) and Phytoene desaturase (Pds) are the first two regulatory enzymes in the carotenoids biosynthetic pathway. The genes Psy and Pds are under transcriptional control in many photosynthetic organisms. In the present study, using quantitative real time- PCR (qRT-PCR), the effects of uncoated and gum-Arabic coated PbS nanoparticles (GA-coated PbS NPs) and light intensity on the mRNA levels of Pds were investigated. Relative to mRNA level of Pds at 100 µmol photon m-2 s-1 light intensity (control culture), 2...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097171/structural-insights-into-the-effects-of-charge-reversal-substitutions-at-the-surface-of-horseradish-peroxidase
#2
Leila Navapour, Navid Mogharrab
Horseradish peroxidase (HRP), has gained significant interests in biotechnology, especially in biosensor field and diagnostic test kits. Hence, its solvent-exposed lysine residues 174, 232, and 241 have been frequently modified with the aim of improving its stability and catalytic efficiency. In this computational study, we investigated the effects of Lys-to-Glu substitutions on HRP structure to model charge-reversal manipulations at the enzyme surface. Simulation results implied that upon these substitutions, the number of stable hydrogen bonds and α-helical content of HRP are increased and the proximal Ca2+ binding pocket becomes more integrated...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097170/microsatellite-ssr-amplification-by-pcr-usually-led-to-polymorphic-bands-evidence-which-shows-replication-slippage-occurs-in-extend-or-nascent-dna-strands
#3
Abasalt Hosseinzadeh-Colagar, Mohammad Javad Haghighatnia, Zahra Amiri, Maryam Mohadjerani, Majid Tafrihi
Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplification which are produced 'stutter products' differing in length from the main products. The purpose of this study is introducing a reliable method to realize SSRs replication slippage...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097169/molecular-characterization-of-argulus-bengalensis-and-argulus-siamensis-crustacea-argulidae-infecting-the-cultured-carps-in-west-bengal-india-using-18s-rrna-gene-sequences
#4
Avijit Patra, Anjan Mondal, Sayani Banerjee, Harresh Adikesavalu, Siddhartha Narayan Joardar, Thangapalam Jawahar Abraham
The present study characterized Argulus spp. infecting the cultured carps using 18S rRNA gene sequences, estimated the genetic similarity among Argulus spp. and established their phylogenetic relationship. Of the 320 fish samples screened, 34 fish (10.6%) had Argulus infection. The parasitic frequency index (PFI) was observed to be high (20%) in Hypophthalmichthys molitrix and Labeo bata. The frequency of infection was high in September (PFI: 17%) and October (PFI: 12.9%). The 18S rRNA sequences of five A. bengalensis (KF583878, KF192316, KM016968, KM016969, and KM016970) and one A...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#5
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097167/expression-purification-and-kinetic-characterization-of-recombinant-benzoate-dioxygenase-from-rhodococcus-ruber-ukmp-5m
#6
Arezoo Tavakoli, Ainon Hamzah, Amir Rabu
In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector. The recombinant plasmid pGEMT-benA was digested by double restriction enzymes BamHI and HindIII to construct plasmid pET28b-benA and was then ligated into Escherichia coli BL21 (DE3)...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097166/molecular-characterization-of-pasteurella-multocida-isolates-obtained-from-poultry-ruminant-cats-and-dogs-using-rapd-and-rep-pcr-analysis
#7
Hesamaddin Shirzad-Aski, Mohammad Tabatabaei
In the present study, Randomly Amplified Polymorphic DNA (RAPD) and Repetitive Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP- PCR) were used to characterize 131 isolates of Pasteurella multocida, originating from different healthy and diseased animal species obtained from several geographical regions of Iran. The RAPD and REP-PCR generated amplified products in the range of 300 to 3400 bp and 200 to 2850 bp, respectively. Among all of the P. multocida isolates, cluster analysis revealed that 63 clusters and nine untypable isolates and 81 clusters and six untypable isolates were produced with RAPD and REP-PCR methods, respectively...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097165/in-silico-comparison-of-iranian-hiv-1-envelop-glycoprotein-with-five-nearby-countries
#8
Maryam Ghafari, Mandana Behbahani, Hassan Mohabatkar
HIV-1 envelope (env) glycoprotein mediates an important role in entry of the virus into the susceptible target cells. As env glycoprotein of HIV-1 is highly variable in the different geographical regions, in the present study, different properties of this protein in Iran are compared with five nearby countries. The sequences of HIV-1 env glycoproteins of Iran, Afghanistan, Russia, Turkey, Pakistan and Saudi Arabia databases were collected from databases. Amino acid composition and physical and chemical properties of the proteins from these countries were studied using Protparam and COPid tools...
June 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097164/molecular-characterization-and-construction-of-an-infectious-clone-of-a-pepper-isolate-of-beet-curly-top-iran-virus
#9
Omid Eini, Ghazal Ebadzad Sahraei, Seyed Ali Akbar Behjatnia
Geminiviruses cause curly top disease, in dicotyledonous plants which constrains host crop production. Beet curly top Iran virus (BCTIV) is a widespread Becurtovirus (family Geminiviridae) in numerous areas within Iran. In this study, we isolated and analyzed a full-length genomic DNA of a new variant of BCTIV from pepper crops in the Kaftark region, east of Shiraz (proposed acronym: BCTIV-Kaf [IR: Kaf:2016:Pepper]). Infected pepper plants showed shortening of internodes, severe interveinal chlorosis, upward leaf rolling and leaf curling...
June 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097163/genetic-polymorphisms-in-the-promoter-region-of-catalase-gene-creates-new-potential-pax-6-and-stat4-response-elements
#10
Khyber Saify
Catalase (CAT, OMIM: 115500) is an endogenous antioxidant enzyme and genetic variations in the regulatory regions of the CAT gene may alter the CAT enzyme activity and subsequently may alter the risk of oxidative stress related disease. In this study, potential influence(s) of the A-21T (rs7943316) and C-262T (rs1001179) genetic polymorphisms in the CAT promoter region, using the ALGGEN-PROMO.v8.3 online software were analyzed. Our findings show that the A allele at the -21 position creates a new potential binding site for PAX-6 and the T allele at the -262 position changes the TFII-I binding site into STAT4 response element...
June 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097162/antioxidant-activity-of-polyphenolic-myricetin-in-vitro-cell-free-and-cell-based-systems
#11
Abolfazl Barzegar
Myricetin (Myc) is one of the most important flavonoids in diet due to its abundance in foods with the highest antioxidant activity. The antioxidant activity of Myc was studied in cell-free and cell-based systems to evaluate the ROS protection efficiency of Myc. The studies were based on the assessment of reducing power of Myc according to ferric ion reduction and intracellular ROS level measurement by assaying the cellular fluorescence intensity using dichlorodihydrofluorescein (DCF) probe as an indicator for ROS in cells...
June 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097161/effects-of-extremely-low-frequency-electromagnetic-field-on-expression-levels-of-some-antioxidant-genes-in-human-mcf-7-cells
#12
Hamideh Mahmoudinasab, Fatemeh Sanie-Jahromi, Mostafa Saadat
In the past three decades, study on the biological effects of extremely low-frequency electromagnetic fields (ELF-EMFs) has been of interest to scientists. Although the exact mechanism of its effect is not fully understood, free radical processes has been proposed as a possible mechanism. This study was designed to evaluate the effect of 50-Hz EMFs on the mRNA levels of seven antioxidant genes (CAT, SOD1, SOD2, GSTO1, GSTM3, MSGT1, and MSGT3) in human MCF-7 cells. The EMF exposure patterns were: 1) 5 min field-on/5 min filed-off, 2) 15 min field-on/15 min field-off, 3) 30 min field-on continuously...
June 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097160/contribution-to-the-molecular-systematics-of-the-genus-capoeta-from-the-south-caspian-sea-basin-using-mitochondrial-cytochrome-b-sequences-teleostei-cyprinidae
#13
Halimeh Zareian, Hamid Reza Esmaeili, Adeleh Heidari, Majid Reza Khoshkholgh, Hamed Mousavi-Sabet
Traditionally, Capoeta populations from the southern Caspian Sea basin have been considered as Capoeta capoeta gracilis. Study on the phylogenetic relationship of Capoeta species using mitochondrial cytochrome b gene sequences show that Capoeta population from the southern Caspian Sea basin is distinct species and receive well support (posterior probability of 100%). Based on the tree topologies obtained from Bayesian and Maximum Likelihood methods, three main groups for the studied Capoeta were detected: Clade I) Capoeta trutta group (the Mesopotamian Capoeta group) including closely related taxa (e...
June 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844021/no-association-between-gstm1-and-gstt1-genetic-polymorphisms-and-susceptibility-to-opium-sap%C3%A2-dependence
#14
Khyber Saify, Mohammad Rashid Khalighinasab, Mostafa Saadat
Glutathione S-transferases (GSTs; EC: 2.5.1.18) are a ubiquitous family of eukaryotic and prokaryotic phase II metabolic isozymes. Genes encoding GSTM1 (OMIM: 138350), and GSTT1 (OMIM: 600436) are members of class mu and theta, respectively. The most common polymorphism in the GSTM1 is a deletion of the whole GSTM1 gene with a lack of enzyme activity. A homozygous deletion in the GSTT1 has also been reported (null genotypes of GSTT1). The aim of the present study was to investigate the association between GSTM1 and GSTT1 polymorphisms and risk of dependency to opium sap...
March 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844020/epigenetics-in-diagnosis-of-colorectal-cancer
#15
REVIEW
Aga Syed Sameer, Saniya Nissar
Colorectal cancer (CRC) is a third most common epithelial carcinoma. CRC is known to develop from the early precancerous lesion to full blown malignancy via definite phases due to cumulative mutations and aberrant methylation of number of genes. The use of serum biomarkers that is non-invasive to discriminate cancer patients from healthy persons will prove to be an important tool to improve the early diagnosis of CRC. This will serve as the boon to the clinical management of the disease.
March 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844019/analysis-of-il-33-gene-polymorphism-rs11792633-c-t-and-risk-of-schizophrenia
#16
Dor Mohammad Kordi-Tamandani, Ahmad Reza Bahrami, Raziye Sabbaghi-Ghale-No, Hanieh Soleimani, Tayebe Baranzehi
Recently, inflammation has been found to be a significant factor in the development of Schizophrenia (SCZ). The aim of the present research was to investigate whether interleukin-33 (IL-33, OMIM: 608678) gene polymorphism (rs11792633, C/T) is associated with the development of SCZ or not. DNA was isolated from the serum of 70 patients with SCZ and 70 healthy controls. The PCR based method was used for detection of the IL-33 polymorphism. The CT (OR=0.05, 95% CI: 0.003-0.057, P<0.001) and TT (OR=0.12, 95% CI: 0...
March 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844018/design-simplified-cloning-and-in-silico-analysis-of-multisite-small-interfering-rna-targeting-cassettes
#17
Bahram Baghban-Kohnehrouz, Shahnoush Nayeri
Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning strategy to develop multisite small interfering RNA (siRNA) cassette from different genes by two cloning steps. In this method, effective siRNA sites in the target messenger RNAs (mRNAs) were determined using in silico analysis and consecutively arranged to reduce length of inverted repeats...
March 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844017/design-of-new-potent-htlv-1-protease-inhibitors-in-silico-study
#18
Mitra Kheirabadi, Javad Maleki, Safieh Soufian, Samaneh Hosseini
HTLV-1 and HIV-1 are two major causes for severe T-cell leukemia disease and acquired immune deficiency syndrome (AIDS). HTLV-1 protease, a member of aspartic acid protease family, plays important roles in maturation during virus replication cycle. The impairment of these proteases results in uninfectious HTLV-1virions.Similar to HIV-1protease deliberate mutations that confer drug resistance on HTLV-1 are frequently seen in this protease. Therefore, inhibition of HTLV-1 protease activity is expected to disrupt HTLV-1's ability to replicate and infect additional cells...
March 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844016/omentin-1-rs2274907-and-resistin-rs1862513-polymorphisms-influence-genetic-susceptibility-to-nonalcoholic-fatty-liver-disease
#19
Leila Kohan, Mehrnush Safarpur, Hamed Abdollahi
Nonalcoholic fatty liver disease (NAFLD) is an obesity-associated disease and dysregulation of adipokines has an important role in its development. Omentin-1 (ITLN1 protein) and resistin are two adipokine secreted from adipose tissue. Single nucleotide polymorphisms in the adipokine genes may affect expression and activity of the adipokine, and thus play a contributory role in NAFLD pathogenesis. The aim of the present study was to investigate the association between omentin-1 rs2274907 (326A/T) and resistin rs1862513 (-420 C/G) polymorphisms and risk of NAFLD in Iranian patients...
March 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/27844015/l-asparaginase-production-in-the-pseudomonas-pseudoalcaligenes-strain-jhs-71-isolated-from-jooshan-hot-spring
#20
Arastoo Badoei-Dalfard
L-asparaginase has lots of medical and industrial applications. Ever since L-asparaginase anti-tumor activity was first demonstrated, its production using microbial systems has attracted considerable attention owing to their cost-effective and eco-friendly nature. The aim of this study is to obtain L-asparaginase producing bacteria and determining the enzyme activity. Samples were picked up from Jooshan hot springs located in the Sirch, Kerman. The L-asparaginase producing bacteria were screened on the agar medium supplied with L-asparagine and phenol red indicator dye (pH-7...
March 2016: Molecular Biology Research Communications
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