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Advances in Medical Mycology (Iran)

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https://www.readbyqxmd.com/read/29707677/patulin-contamination-in-apple-products-marketed-in-shiraz-southern-iran
#1
Ali Poostforoushfard, Ahmad R Pishgar, Enayat Berizi, Hasti Nouraei, Zahra Sobhani, Rohallah Mirzaie, Kamiar Zomorodian
Background and Purpose: Patulin is one of the important mycotoxins, produced by a wide range of molds, including Penicillium , Aspergillus, and Bysochlamys. Patulin is mainly found in the rotten parts of fruits and vegetables, such as apples, pears, peach, apricots, and grapes. Currently, the Codex Alimentarius and Food and Drug Administration have recommended a maximum level of 50 µg/L patulin for apple products. The purpose of this study was to investigate patulin contamination of apple juice and cans in 75 samples collected from 15 manufacturers in Shiraz, southern Iran...
December 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707676/mycological-aspects-of-onychomycosis-in-khuzestan-province-iran-a-shift-from-dermatophytes-towards-yeasts
#2
Mahnaz Fatahinia, Sima Jafarpour, Abdollah Rafiei, Simin Taghipour, Koichi Makimura, Ali Rezaei-Matehkolaei
Background and Purpose: Onychomycosis is fungal infection of the nails with an overall increasing incidence, worldwide. The epidemiological aspects of onychomycosis in Khuzestan, Iran, have not been established. In this study, we aimed to evaluate the clinical and mycological status of fungal nail infection in Khuzestan Province, Iran. Materials and Methods: The study population included 433 patients (143 males vs. 290 females). Nail samples underwent primary direct microscopy and culture...
December 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707675/identification-of-candida-species-isolated-from-vulvovaginitis-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#3
Majid Alizadeh, Anna Kolecka, Teun Boekhout, Hossein Zarrinfar, Mohamad A Ghanbari Nahzag, Parisa Badiee, Ali Rezaei-Matehkolaei, Abdolmajid Fata, Somayeh Dolatabadi, Mohammad J Najafzadeh
Background and Purpose: Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify Candida isolates by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) from women with vulvovaginitis that were referred to Ghaem Hospital, Mashhad, Iran. Materials and Methods: This study was conducted on 65 clinical samples isolated from women that were referred to Ghaem Hospital. All specimens were identified using phenotyping techniques, such as microscopy and culture on Sabouraud dextrose agar and corn meal agar...
December 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707674/microsatellite-genotyping-of-clinical-candida-parapsilosis-isolates
#4
Hamid Badali, Sassan Rezaie, Jacques F Meis, Setareh Agha Kuchak Afshari, Mona Modiri, Ferry Hagen, Maryam Moazeni, Rasoul Mohammadi, Sadegh Khodavaisy
Background and Purpose: Candida parapsilosis is a predominant species found in nosocomial infection, particularly in hospitalized patients. The molecular epidemiology of the clinical strains of this species has not been well studied. The present study was performed with the aim of investigating the microsatellite genotyping of Candida parapsilosis among the Iranian clinical isolates. Materials and Methods: This study was conducted on 81 independent clinical C. parapsilosis isolates that were genotyped by using a panel of six microsatellite markers...
December 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707673/phytochemical-analysis-and-docking-study-of-compounds-present-in-a-polyherbal-preparation-used-in-the-treatment-of-dermatophytosis
#5
Nagesh Simhadri Vsdna, Muthuchamy Muniappan, Iyanar Kannan, Subramanyam Viswanathan
Background and Purpose: Soleshine is a polyherbal preparation established in the market for the treatment of cracks and tinea pedis, which is applied externally. This preparation is composed of the extracts of indigenous plants, namely Azadirachta indica, Lawsonia alba, and Shorea robusta , mixed with castor oil and sesame oil. In the present study, an attempt was made to identify the constituents of soleshine and identify some potential drug-like molecules that can inhibit important drug targets of the dermatophytes using molecular docking method...
December 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707672/evaluation-of-esterase-and-hemolysin-activities-of-different-candida-species-isolated-from-vulvovaginitis-cases-in-lorestan-province-iran
#6
Maryam Noori, Mohammad Dakhili, Asghar Sepahvand, Nader Davari
Background and Purpose: Annually affecting millions of women, vulvovaginal candidiasis (VVC) is commonly described by signs and symptoms of vulvovaginal inflammation in the presence of  Candida  species. Today, the detection of the virulence factors plays a major role in the understanding of pathogenesis of candidiasis and helps produce new anticandidial drugs to improve its treatment efficiency. Herein, we aimed to evaluate the esterase and hemolysin activities of the vaginal isolates of Candida and their relationship with the presence of VVC...
December 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707671/otomycosis-in-damascus-syria-etiology-and-clinical-features
#7
Mohammad T Ismail, Abeer Al-Kafri, Mazen Ismail
Background and Purpose: Otomycosis is a fungal infection that frequently involves the external auditory canal. The epidemiologic data on the etiologic agents of otomycosis in Syria are very limited. In this study, we aimed to determine the fungal agents, gender distribution, and clinical presentation of otomycosis. Materials and Methods: Two hundred and ninety nine patients (153 [51.17%] male and 146 [48.83%] female) clinically prediagnosed as otomycosis were studied at Al-mouassat University Hospital and ENT Crescent Syrian Clinic...
September 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707670/morphological-changes-and-induction-of-antifungal-resistance-in-aspergillus-fumigatus-due-to-different-co2-levels
#8
Sima Darabian, Sayed Jamal Hashemi, Sadegh Khodavaisy, Somayeh Sharifynia, Mohammad Kord, Maryam Akbari Dana, Farzad Aala, Sassan Rezaie
Background and Purpose: Aspergillosis is one of the most common opportunistic fungal infections in immunocompromised and neutropenic patients. Aspergillus fumigatus ( A. fumigatus ) is the most common causative agent of this infection. Due to variable CO2 concentrations that pathogens are exposed to during the infection process and to understand the role of CO2 , we examined the effects of various CO2 concentrations as one of the environmental factors on morphological changes and induction of antifungal resistance in A...
September 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707669/effect-of-biogenic-selenium-nanoparticles-on-erg11-and-cdr1-gene-expression-in-both-fluconazole-resistant-and-susceptible-candida-albicans-isolates
#9
Nasrin Parsameher, Sassan Rezaei, Sadegh Khodavasiy, Samira Salari, Sanaz Hadizade, Mohammad Kord, Seyed Amin Ayatollahi Mousavi
Background and Purpose: Candida albicans is the most common Candida species (sp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interactions, the identification of new antifungal agents seems essential. In this study, we assessed the antifungal effects of biogenic selenium nanoparticles on C. albicans and determined the expression of ERG11 and CDR1 genes...
September 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707668/comparison-of-chromagar-polymerase-chain-reaction-restriction-fragment-length-polymorphism-and-polymerase-chain-reaction-fragment-size-for-the-identification-of-candida-species
#10
Zahra Jafari, Marjan Motamedi, Nilufar Jalalizand, Gholam R Shokoohi, Arezu Charsizadeh, Hossein Mirhendi
Background and Purpose: The epidemiological alteration in the distribution of Candida species, as well as the significantly increasing trend of either intrinsic or acquired resistance of some of these fungi highlights the need for a reliable method for the identification of the species. Polymerase chain reaction (PCR) is one of the methods facilitating the quick and precise identification of Candida species. The aim of this study was to compare the efficiency of CHROMagar, PCR-restriction fragment length polymorphism (PCR-RFLP), and PCR-fragment size polymorphism (PCR-FSP) assays in the identification of Candida species to determine the benefits and limitations of these methods...
September 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707667/identification-of-fungal-causative-agents-of-rhinosinusitis-from-mashhad-iran
#11
Mohammad J Najafzadeh, Karim Jalaeian Samani, Jos Houbraken, Majid Alizadeh, Abdolmajid Fata, Ali Naseri, Hossein Zarrinfar, Mehdi Bakhshaee
Background and Purpose: Rhinosinusitis is a common disorder, influencing approximately 20% of the population at some time of their lives. It was recognized and reported with expanding recurrence over the past two decades worldwide. Undoubtedly, correct diagnosis of fungi in patients with fungal rhinosinusitis affects the treatment planning and prognosis of the patients. Identification of the causative agents using the standard mycological procedures remains difficult and time-consuming...
September 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29707666/detection-of-ochratoxin-a-in-human-breast-milk-in-jiroft-city-south-of-iran
#12
Ali Kamali, Sareh Mehni, Mohadeseh Kamali, Mehdi Taheri Sarvtin
Background and Purpose: Breastfeeding plays an important role in the growth and development of infants. However, breast milk may be contaminated with various mycotoxins. Ochratoxin A is one of the most important mycotoxins with nephrotoxic, carcinogenic, teratogenic, genotoxic, and immunotoxic properties. Thus, we carried out this study to determine the concentration of ochratoxin A in human breast milk in Jiroft, Kerman Province, south of Iran. Materials and Methods: Eighty-four human breast milk samples were collected from mothers visiting the number one clinic in Jiroft city...
September 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354779/first-case-of-superficial-infection-due-to-naganishia-albida-formerly-cryptococcus-albidus-in-iran-a-review-of-the-literature
#13
S Aghaei Gharehbolagh, M Nasimi, S Agha Kuchak Afshari, Z Ghasemi, S Rezaie
Background and Purpose: Naganishia albida (formerly Cryptococcus albidus ) is a non-neoformans cryptococcal species rarely isolated as a human pathogen. Case report: Herein, we present the case of a 26-year-old Iranian man with a superficial cutaneous lesion in the axilla. The initial treatment for pityriasis versicolor by clotrimazole was unsuccessful. We performed skin sampling based on the standard protocol and conducted further investigations by the conventional laboratory tests and molecular analysis of the skin samples...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354778/the-efficacy-of-gaseous-ozone-against-different-forms-of-candida-albicans
#14
M Zargaran, M Fatahinia, A Zarei Mahmoudabadi
Background and Purpose: Ozone is an inorganic molecule with effective antimicrobial properties. Clinical treatment of ozonated water was used for the elimination of Candida albicans , Enterococcus faecalis , endotoxins, and biofilms from root canals. In addition, its therapeutic effects for tinea pedis, ulcers, and leishmaniasis were investigated. The purpose of the present study was to evaluate the fungicidal effects of ozone on different forms of C. albicans . In addition, antifungal susceptibility profile of strains was assessed before and after exposure to ozone...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354777/isolation-and-molecular-characterization-of-cryptococcus-species-isolated-from-pigeon-nests-and-eucalyptus-trees
#15
A Kamari, A Sepahvand, R Mohammadi
Background and Purpose: Cryptococcus species are pathogenic and non-pathogenic basidiomycete yeasts that are found widely in the environment. Based on phenotypic methods, this genus has many species; however, its taxonomy is presently being re-evaluated by modern techniques. The Cryptococcus species complex includes two sibling taxa of Cryptococcus neoformans and Cryptococcus gattii . We aimed to investigate the possible distribution of Cryptococcus species in pigeon nests and Eucalyptus trees in Ilam, Iran, using molecular techniques...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354776/isolation-of-keratinophilic-fungi-from-the-soil-of-greater-tunb-abu-musa-and-sirri-islands-in-the-persian-gulf-iran
#16
M Nosratabadi, P Kordbacheh, R Kachuei, M Safara, S Rezaie, Ma Afshari
Background and Purpose: Keratinophilic fungi are among the important groups of fungi living in the soil. This study aimed to isolate and identify keratinophilic fungi from the soil of three Iranian islands, namely Greater Tunb, Abu Musa, and Sirri, located in the Persian Gulf using morphological and molecular (polymerase chain reaction) methods. Materials and Methods: In this study, a total of 60 soil samples were collected from the three islands of Greater Tunb, Abu Musa, and Sirri...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354775/-in-vitro-antimicrobial-effect-of-different-root-canal-sealers-against-oral-pathogens
#17
A Monajemzadeh, S Ahmadi Asoor, S Aslani, B Sadeghi-Nejad
Background and Purpose: Root canal therapy is the primary method for the treatment of an infected pulp in modern dentistry. The main aim of endodontic treatment is the elimination of bacteria and their products from infected root canals. In this study, we attempted to investigate the antimicrobial activity of three root canal sealers against oral pathogens. Materials and Methods: The antimicrobial effectiveness of three endodontic sealers with different chemical compositions, namely resin (AH 26), zinc oxide eugenol (ZOE), and mineral trioxide aggregate (MTA), against Candida albican s, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans , and Lactobacillus casei was assayed by agar well diffusion method (AWDM)...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354774/-in-vitro-antifungal-activity-of-essential-oils-extracted-from-plants-against-fluconazole-susceptible-and-resistant-candida-albicans
#18
F Katiraee, S Ahmadi Afshar, S F Rahimi Pirmahalleh, H Shokri
Background and Purpose: Candida albicans is the most common cause of candidal infections. Various studies have shown drug resistance among C. albicans isolates; thus, it is necessary to discover replacement treatments for Candida infections. In this study, we aimed to compare the effects of different essential oils against azoles-resistant and azoles-susceptible isolates. Materials and Methods: Twenty fluconazole-resistant and 20 susceptible C. albicans isolates obtained from oral, vaginal, and cutaneous tissues of patients with candidiasis were evaluated...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29302628/intestinal-microsporidiosis-in-iran-infection-in-immune-compromised-and-immunocompetent-patients
#19
M Nooshadokht, I Sharifi, M A Mohammadi, M Pirestani, A Afgar, A Mahootchi, S Salari
Background and Purpose: Gastroenteritis and the clinical profile caused by Microsporidia , an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to the limitations of the microscopy-based methods, several DNA-based methods such as polymerase chain reaction (PCR) have recently been developed to enhance diagnosis sensitivity. Therefore, we sought to evaluate the rate of infection in immunocompromised patients as compared with immunocompetent patients in Kerman, Iran...
March 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29302627/in-vitro-antitumor-activity-of-patulin-on-cervical-and-colorectal-cancer-cell-lines
#20
M Abastabar, A Akbari, J Akhtari, M T Hedayati, T Shokohi, H Mehrad-Majd, H Ghalehnoei, S Ghasemi
Background and Purpose: Patulin is a mycotoxin produced by some molds, especially Aspergillus and Penicilium , and is responsible for mycotoxicosis in animals and humans.There is still not very detailed data about the anti-cancer potency of patulin, but some reports demonstrated that it induces cellular apoptosis and toxicity. Materials and Methods: To determine the efficacy of patulin as a therapeutic strategy for cervical and colorectal cancers, we investigated its effects on HeLa,SW-48, and MRC-5 cell lines...
March 2017: Advances in Medical Mycology (Iran)
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