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EXCLI Journal

Aisha Siddiquah, Syed Salman Hashmi, Sadaf Mushtaq, Sullivan Renouard, Jean Philippe Blondeau, Rashda Abbasi, Christophe Hano, Bilal Haider Abbasi
Little is known about biogenically synthesized Zinc oxide nanoparticles (ZnONPs) from Isodon rugosus . Synthesis of metal oxide NPs from essential oil producing medicinal plants results in less harmful side effects to the human population as compared to chemically synthesized NPs. In this article, we report biogenic synthesis of ZnONPs from in vitro derived plantlets and thidiazuron (TDZ) induced callus culture of Isodon rugosus . Synthesized NPs were characterized using UV-spectra, XRD, FTIR, SEM and EDX. Furthermore, the NPs were evaluated for their potential cytotoxic (against HepG2 cell line) and antimicrobial (against drug resistant Staphylococcus epidermidis , Bacillus subtilis , Klebsiella pneumoniae and Pseudomonas aeruginosa ) activities...
2018: EXCLI Journal
Yasser A Ahmed, Safwat Ali, Ahmed Ghallab
Animal hair examination at a criminal scene may provide valuable information in forensic investigations. However, local reference databases for animal hair identification are rare. In the present study, we provide differential histological analysis of hair of some domestic animals in Upper Egypt. For this purpose, guard hair of large ruminants (buffalo, camel and cow), small ruminants (sheep and goat), equine (horse and donkey) and canine (dog and cat) were collected and comparative analysis was performed by light microscopy...
2018: EXCLI Journal
Fakhredin Saba, Masoud Soleimani, Saeid Abroun
Bone is one of the most common sites of complication in multiple myeloma (MM) progression and bone remodeling gets definitively perturbed during disease progression. Hypoxia and miR-210 play an important role in hematological malignancies. In an attempt to elucidate the specificity of the pathways of hypoxia and miR-210 in suppression of osteoblastic differentiation in MM patients, we examined the effect of miR-210 and hypoxia on expression of important cytokines and genes of myeloma cells. Differentiation of BM-MSCs towards osteoblastic cells in response to microvesicles (MVs) was also investigated...
2018: EXCLI Journal
Kannika Permpoonputtana, Patlada Tangweerasing, Sujira Mukda, Parichart Boontem, Chutikorn Nopparat, Piyarat Govitrapong
Aging is often accompanied by a decline in cognitive function in conjunction with a variety of neurobiological changes, including neuroinflammation. Melatonin is a key endogenous indoleamine secreted by the pineal gland that plays a crucial role in the regulation of circadian rhythms, is a potent free radical scavenger, has anti-inflammatory activity and serves numerous other functions. However, the role of melatonin in sterile inflammation in the brain has not been fully investigated. In the present study, we investigated the neuroinflammation status in aged mouse brains...
2018: EXCLI Journal
Maryam Yazdi, Hamidreza Roohafza, Awat Feizi, Nizal Sarafzadegan
Objectives : The current study aimed at evaluating the major domains of stressful life events and their association with psychological problems profile in a large sample of Iranian industrial manufacturing employees. Methods : In a cross-sectional study, 3,063 participants were randomly selected from 16,000 employees working in a big industrial company in Isfahan, Iran. Three common psychological problems i.e. depression, anxiety and psychological distress were evaluated using Persian validated version of Hospital Anxiety and Depression Scale (HADS) and 12-item General Health Questionnaire (GHQ-12), respectively...
2018: EXCLI Journal
Abdolkarim Moazeni-Roodi, Mohammad Hashemi
Many studies examined the association between miR-124-1 rs531564 polymorphism and the risk of some human cancers, but the findings remain controversial. This update meta-analysis aimed to validate the association between rs531564 polymorphism of miR-124-1 and cancer risk. Eligible studies including 6,502 cancer cases and 7,213 controls were documented by searching Web of Science, PubMed, Scopus, and Google scholar databases. Pooled odds ratios (ORs) with 95 % confidence intervals (CIs) were estimated to quantitatively evaluate the association between rs531564 variant and cancer risk...
2018: EXCLI Journal
Ramesh L Sawant, Jyoti B Wadekar, Santosh B Kharat, Hitakshi S Makasare
A series of thiazolidine derivatives were designed by docking into PPAR-γ active site. The structure of target was obtained from the protein data bank (PDB ID P37231). A library of 200 molecules was prepared on random basis. Molecular docking studies were performed using VLife MDS 4.3 software. After molecular docking studies, the 4-substituted-6-methyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carboxylic acid N-[4-(2,4-dioxo-thiazolidin-5-ylidenemethyl)-phenyl]-hydrazides ( 4a-4h ) were selected for synthesis...
2018: EXCLI Journal
Azadeh Mohammadi-Farsani, Mehryar Habibi-Roudkenar, Majid Golkar, Mohammad Ali Shokrgozar, Ali Jahanian-Najafabadi, Hossein KhanAhmad, Samira Valiyari, Saeid Bouzari
The NGR peptide is one of the well-known peptides for targeting tumor cells. It has the ability to target aminopeptidase N (CD13) on tumor cells or the tumor vascular endothelium. In this study, the NGR peptide was used for targeting A subunit of the Shiga toxin to cancer cells. The cytotoxic effect of the A-NGR fusion protein was assessed on HT1080, U937, HT29 cancer cells and MRC-5 normal cells. For this purpose, cells were treated with different concentrations of A-NGR (0.5-40 µg/ml). The evaluation of cell viability was achieved by MTT assay...
2018: EXCLI Journal
Navid Ghasemzadeh, Fatemeh Pourrajab, Ali Dehghani Firoozabadi, Seyedhossein Hekmatimoghaddam, Fatemeh Haghiralsadat
Human mesenchymal stem cells (hMSCs) have remarkable potential for use in regenerative medicine. However, one of the great challenges is preserving their potency for long time. This study investigated the effect of miRNA ectopic expression on their proliferation and also on the expression level of Parp1 as an epigenetic switch preserving pluripotency in hMSCs. A cationic liposome was prepared as an efficient carrier for miRNA delivery. The miRNA loading efficiency and physical stability of vesicles were measured, and their scanning electron microscopic shapes determined...
2018: EXCLI Journal
Mohd Noor Azreey Mukri, Wei-Yen Kong, Ruslinda Mustafar, Syahrul Sazliyana Shaharir, Shamsul Azhar Shah, Abdul Halim Abdul Gafor, Rozita Mohd, Rizna Abdul Cader, Lydia Kamaruzaman
Introduction: Hyperuricemia is associated with chronic kidney disease (CKD) progression and poor cardiovascular outcomes. We studied the effect of febuxostat on estimated glomerular filtration rate (eGFR), proteinuria and monitored the safety profile of the medication. Material and Methods: This is a prospective open-label, randomized study in CKD stage 3 and 4 patients with diabetic nephropathy and asymptomatic hyperuricemia. Patients were randomized into febuxostat 40 mg daily and no treatment group using block randomization method and were followed up for 6 months...
2018: EXCLI journal
Chadinee Thippakorn, Chartchalerm Isarankura-Na-Ayudhya, Supitcha Pannengpetch, Patcharee Isarankura-Na-Ayudhya, Nalini Schaduangrat, Chanin Nantasenamat, Virapong Prachayasittikul
Pseudomonas aeruginosa is known to produce multiple types of pigment which are involved in its pathogenicity and survival in certain environments. Herein, we reported the identification of P. aeruginosa dark-brown hyperpigmented (HP) strains which have been isolated from clinical samples. In order to study the role of these dark-brown containing secretions, alterations of metabolic processes and cellular responses under microenvironment of this bacterial pathogen, two-dimensional gel electrophoresis (2-DE) in conjunction with peptide mass fingerprinting (PMF) were performed...
2018: EXCLI Journal
Seung-Shick Shin, Myeong-Cheol Ko, Yu-Jin Park, Byungdoo Hwang, Sung Lyea Park, Wun-Jae Kim, Sung-Kwon Moon
Hydrangenol is a dihydroisocoumarin that is mainly obtained from Hydrangea macrophylla . Recently, hydrangenol has garnered attention since several studies have reported that it has anti-inflammatory, anti-allergic, anti-diabetic, and anti-malarial activities. However, there have been few studies on the effect of hydrangenol on oncogenesis. In this study, we evaluated the anti-cancer activity of hydrangenol against the EJ bladder cancer cell line. Hydrangenol significantly inhibited the proliferation of EJ cells in a dose-dependent manner with an IC50 of 100 µM...
2018: EXCLI Journal
Kashif Aziz, Amir Shahbaz, Muhammad Umair, Isaac Sachmechi
Avelumab is an anti-PD-L1 (programmed death-ligand 1) immune checkpoint inhibitor (ICIs) and the monoclonal antibody that constitutes a major development in the immunotherapy of cancer. In 2017, The European Medicine Agency (EMA) approved it as an orphan drug for treatment of gastric cancer. Avelumab has recently been approved in the United States, Europe and Japan for treatment of metastatic Merkel cell carcinoma (MCC). Avelumab inhibits the interaction of Programmed cell death protein 1 (PD-1) on immune cells with PD-L1 on tumor cells, thus banishing immunosuppressive signals and leading to enhanced immune cell activation...
2018: EXCLI Journal
Mohammad Zangooei, Mitra Nourbakhsh, Mohammad Hossein Ghahremani, Reza Meshkani, Azam Khedri, Amir Shadboorestan, Hajar Shokri Afra, Shiva Shahmohamadnejad, Hossein Mirmiranpour, Shahnaz Khaghani
Obesity is associated with higher postmenopausal breast cancer incidence. Visfatin level alteration is one of the mechanisms by which obesity promotes cancer. Ligand-independent activation of estrogen receptor alpha (ERα) is also associated with carcinogenesis. The activity of ERα is modulated through phosphorylation on multiple sites by a number of protein kinases. Here we investigated the effect of visfatin as a novel adipocytokine on the phosphorylation and activity of ERα in MCF-7 breast cancer cells...
2018: EXCLI Journal
Samaneh Nakhaee, Omid Mehrpour
No abstract text is available yet for this article.
2018: EXCLI Journal
Junli Wang, Wenjing Xiao, Wanyi Chen, Yonghe Hu
The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) have been recognized as inflammatory markers and used as prognostic makers in various cancers. The present study sought to investigate the prognostic role of NLR and PLR in Chinese patients with glioma. Clinical data, including NLR, PLR and overall survival (OS), were collected from 112 patients who underwent surgery to treat primary glioma. Kaplan-Meier survival analysis as well as uni- and multivariate Cox regression were performed to examine potential associations of preoperative NLR and PLR with OS...
2018: EXCLI Journal
Ahlem Soussi, Manel Gargouri, Amel Akrouti, Abdelfattah El Feki
Lead (Pb) intoxication remains a major health hazard causing various deleterious effects especially on renal and hematologic system. The current study elucidated the potential protective effect of JRVO against nephrotoxicity induced by lead. Male rats were randomly divided into three groups: group one (control) received ad libitum distilled water and 1 mL of saline solution (0.9 %) given by intra-peritoneal (i.p) injection, group two (Pb) was kept on tap distilled water and animals were i.p, injected daily with lead every two days from day five until day ten, namely the sacrifice day, and group three (Pb+J) was administered by intra-peritoneal injection of Pb with the same dose and same way with Group two, while JRVO extract was administered daily by gavage during ten days...
2018: EXCLI Journal
Mohammad Karimian, Younes Aftabi, Tahereh Mazoochi, Faezeh Babaei, Tahereh Khamechian, Hossein Boojari, Hossein Nikzad
Survivin is a member of the apoptosis inhibitor protein family and its polymorphisms may lead to susceptibility to cancer. The aim of this study was to investigate the possible association of c.-31G>C (rs9904341), c.454G>A (rs2071214), c.*148T>C (rs2239680) and c.*571T>C (rs1042489) polymorphisms of survivin gene with prostate cancer risk and provide some justification using in silico analysis. The 157 men with prostate cancer and 145 healthy controls were included in a case-control study. The studied polymorphisms were genotyped using PCR-RFLP method...
2018: EXCLI Journal
Vichanan Yamkamon, Benjarong Phakdee, Sakda Yainoy, Thummaruk Suksrichawalit, Tararat Tatanandana, Premsant Sangkum, Warawan Eiamphungporn
An enzyme-coupled colorimetric assay for quantification of urinary sarcosine was developed. The proposed method is a specific reaction based on hydrogen peroxide (H2 O2 ) formation via sarcosine oxidase (SOX). The liberated H2 O2 reacts with Amplex Red in the presence of horseradish peroxidase (HRP) to produce the red-fluorescent oxidation product, resorufin, which can be measured spectrophotometrically (OD570). The method was performed in the 96-well microtiter plate. Reaction conditions, such as pH and reaction time were optimized...
2018: EXCLI Journal
Somchai Boonpangrak, Tanawut Tantimongcolwat, Lertyot Treeratanapiboon, Pairoj Leelahakul, Virapong Prachayasittikul
This study aimed to investigate the influence of lifestyle behaviors on the vitamin C levels in the circulating blood of the Thai population in Bangkok Metropolitan. The participants (n=250) included community workers (i.e., construction and business office workers) from the Bangkok Metropolitan, and the participants were placed in various behavior and lifestyle groups (Group I: reference; Group II: alcohol drinkers; Group III: outdoor workers; Group IV: smokers; and Group V: combined). The results showed that the lowest and highest vitamin C intakes were 7 and 27 mg/day in Groups IV and III, respectively...
2018: EXCLI Journal
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