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Iranian Journal of Biotechnology

Lamia Medouni-Haroune, Farid Zaidi, Sonia Medouni-Adrar, Sevastianos Roussos, Samia Azzouz, Véronique Desseaux, Mouloud Kecha
BACKGROUND: Olive pomace, as the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production. OBJECTIVES: Actinobacteria isolates were separated from an Algerian soil under olive pomace cultivation and were evaluated for their lignocellulolytic enzymes production. MATERIALS AND METHODS: Isolates of Actinobacteria were separated from soils around oil mills using four isolation media, among them three were enriched by olive pomace...
March 2017: Iranian Journal of Biotechnology
Arezoo Tavakoli, Ainon Hamzah
BACKGROUND: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. OBJECTIVES: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized. MATERIALS AND METHODS: The xylC was amplified and cloned in E. coli. The recombinant plasmid pGEMT-xylC was digested by NdeI and HindIII to construct plasmid pET28b-xylC and transformed in E...
March 2017: Iranian Journal of Biotechnology
Bahareh Attaran, Tahereh Falsafi
BACKGROUND: A few reports confirm the ability of Helicobacter pylori to form biofilm. However, conclusive data do not exist concerning the factors that favor this ability. OBJECTIVES: Evaluation of the factors associated with the biofilm formation ability of H. pylori including bacterial, physical and chemical, and environmental factors was the research's aim. MATERIALS AND METHODS: H. pylori isolates from gastric biopsy specimens of patients infected chronically were screened for biofilm formation ability...
March 2017: Iranian Journal of Biotechnology
Kourosh Maboudi, Seyedeh Marzieh Hosseini, Mina Sepahi, Hashem Yaghoubi, Shahin Hadadian
BACKGROUND: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require specifi c antibodies. High cost, inappropriate shipping (cold chain failures), reduced sensitivity and thus poor detection performance are common pitfalls of using commercial kits for performing immunological tests...
March 2017: Iranian Journal of Biotechnology
Niloofar Zandvakili, Mohammadreza Zamani, Mostafa Motallebi, Zahra Moghaddassi Jahromi
BACKGROUND: Plants have various defense mechanisms such as production of antimicrobial peptides, particularly pathogenesis related proteins (PR proteins). PR10 family is an essential member of this group, with antifungal, antibacterial and antiviral activities. OBJECTIVE: The goal of this study is to assess the antifungal activity of maize PR10 against some of fungal phytopathogens. MATERIALS AND METHODS: Zea mays PR10 gene (TN-05-147) was cloned from genomic DNA and cDNA and overexpressed in Escherichia coli...
March 2017: Iranian Journal of Biotechnology
Habib Rezapoor, Mahnaz Aghdasi, Hamid Reza Sadeghipoor
BACKGROUND: TRR14 protein is a small protein, a member of a multigene family in Arabidopsis which was found as the fi rst protein during screening seedlings for their resistant to the trehalose sugar. OBJECTIVES: A number of TRR14-overexpressing plants were subjected to the characterization in the present research, among which, the associated morphological features and changes accompany growth pattern and photosynthesis related parameters. MATERIALS AND METHODS: TRR14 gene was isolated from Arabidopsis Thaliana and cloned into the pBin-35S vector...
March 2017: Iranian Journal of Biotechnology
Behzad Hajieghrari, Naser Farrokhi, Bahram Goliaei, Kaveh Kavousi
BACKGROUND: Micro RNAs (miRNAs) are a pivotal part of non-protein-coding endogenous small RNA molecules that regulate the genes involved in plant growth and development, and respond to biotic and abiotic environmental stresses posttranscriptionally. OBJECTIVE: In the present study, we report the results of a systemic search for identification of new miRNAs in B. rapa using homology-based ESTs (Expressed Sequence Tags) analysis and considering a series of fi ltration criteria...
March 2017: Iranian Journal of Biotechnology
Zahra Zamanzadeh, Mitra Ataei, Seyed Massood Nabavi, Ghasem Ahangari, Mehdi Sadeghi, Mohammad Hosein Sanati
BACKGROUND: Multiple sclerosis (MS) is the most common autoimmune disease of the central nervous system (CNS). The main cause of the MS is yet to be revealed, but the most probable theory is based on the molecular mimicry that concludes some infections in the activation of T cells against brain auto-antigens that initiate the disease cascade. OBJECTIVES: The Purpose of this research is the prediction of the auto-antigen potency of the myelin proteolipid protein (PLP) in multiple sclerosis...
March 2017: Iranian Journal of Biotechnology
Lin Hua, Hong Xia, Wei-Ying Zheng, Li An
BACKGROUND: Transforming growth factor (TGF)-β is over-expressed in a wide variety of cancers such as lung adenocarcinoma. TGF-β plays a major role in cancer progression through regulating cancer cell proliferation and remodeling of the tumor micro-environment. However, it is still a great challenge to explain the phenotypic effects caused by TGF-β stimulation and the effect of TGF-β stimulation on tumor micro-environment. OBJECTIVES: To address this issue, in the present study we used two time-course microarray data in human lung adenocarcinoma cells and applied bioinformatics methods to explore the gene regulation network responding to TGF-β stimulation in lung adenocarcinoma cells...
March 2017: Iranian Journal of Biotechnology
Salim Morammazi, Ali Akbar Masoudi, Rasoul Vaez Torshizi, Abbas Pakdel
BACKGROUND: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired. OBJECTIVES: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed high and low breeding values. MATERIALS AND METHODS: The breeding values of the animals were estimated first by applying multi-trait random regression model...
December 2016: Iranian Journal of Biotechnology
Haleh Salimi, Masoud Bahar, Aghafakhr Mirlohi, Majid Talebi
BACKGROUND: Potato has a narrow genetic base which is due to its development, as it takes its genetic root from a few genotypes originated from South America. OBJECTIVES: The objective of this study was to assess the genetic relationships among potato (Solanum tuberosum L.) genotypes originated from different geographical regions. MATERIALS AND METHODS: This study has rendered 25 useful SSRs and EST-SSRs that were located in pre-existing genetic maps, fingerprinted in a collection of the 47 potato genotypes from America, Europe and Iran...
December 2016: Iranian Journal of Biotechnology
Sakineh Abbasi, Naser Safaie, Masoud Shams-Bakhsh, Samira Shahbazi
BACKGROUND: Random induced mutation by gamma radiation is one of the genetic manipulation strategies to improve the antagonistic ability of biocontrol agents. OBJECTIVES: This study aimed to induce mutants with more sporulation, colonization rate leading to enhanced antagonistic ability (in vitro assay) comparing to wild type (WT) and the assessment of genetic differences (in situ evaluation) using molecular markers. The superior mutants could be appropriate biocontrol agents against soil borne fungal diseases...
December 2016: Iranian Journal of Biotechnology
Kamal Fouad Abdellatif, Ragaa Hamouda Abdelfattah, Mostafa Sayed Mostafa El-Ansary
BACKGROUND: Root-knot nematodes are known to cause significant damage to eggplants. New approaches by green silver nanoparticles (GSN) are used to control plant-parasitic nematode to avoid chemical nematicide hazards. OBJECTIVES: Analyses of the incorporation of different concentrations of nanoparticles on two different algae (Ulva lactuca and Turbinaria turbinata) were carried out. Furethermore, the effect of GSN on the eggplant DNA profile was studied using RAPD and EST molecular markers...
December 2016: Iranian Journal of Biotechnology
Warawan Eiamphungporn, Sakda Yainoy, Virapong Prachayasittikul
BACKGROUND: Human Cu/Zn superoxide dismutase (hSOD1) is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu(2+) insufficiency at protein active site, leading to low solubility and enzymatic activity. OBJECTIVES: The effect of co-expressed human copper chaperone (hCCS) to enhance the solubility and enzymatic activity of hSOD1 in E. coli was investigated in the presence and absence of Cu(2+)...
December 2016: Iranian Journal of Biotechnology
Yongmin Yan, Wenqian Jiang, Jingwen Liu, Wenrong Xu, Hui Qian
BACKGROUND: Phosphodiesterase 3A (PDE3A) and phosphodiesterase 3B (PDE3B) play a critical role in the regulation of intracellular level of adenosine 3´,5´-cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3´,5´-cyclic monophosphate (cyclic GMP, cGMP). Subsequently PDE3 inhibitors have shown to relax vascular and inhibit platelet aggregation in cardiovascular disease. OBJECTIVES: In this study, our aim was to establish a method of expression for the recombinant human PDE3A and PDE3B proteins in insect cells using baculovirus expression system in order to investigate the activity of the expressed PDE3A and PDE3B proteins...
December 2016: Iranian Journal of Biotechnology
Masoud Reza Seyfi Abad Shapouri, Pezhman Mahmoodi, Dariush Gharibi, Masoud Ghorbanpoor, Sara Yaghoubi, Elham Rezaei, Mohammad Rashno, Neda Mehravar
BACKGROUND: Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures. OBJECTIVES: Molecular cloning and expression of SPA followed by the purification and conjugation of the recombinant protein to peroxidase enzyme...
December 2016: Iranian Journal of Biotechnology
Nihan Özengin, Ayse Elmaci
BACKGROUND: There is growing interest in the natural and constructed wetlands for wastewater treatment. While nutrient removal in wetlands has been extensively investigated, information regarding the degradation of the pharmaceuticals and personal care products (PPCPs) has only recently been emerging. PPCPs are widely distributed in urban wastewaters and can be removed to some extent by the constructed wetlands. The medium-term (3-5 years) behavior of these systems regarding PPCP removal is still unknown...
December 2016: Iranian Journal of Biotechnology
Kamran Mousavi Hosseini, Mehran Ghasemzadeh
CONTEXT: The major motivation for the preparation of the plasma derived biological medicine was the treatment of casualties from the Second World War. Due to the high expenses for preparation of plasma derived products, achievement of self-sufficiency in human plasma biotechnological industry is an important goal for developing countries. EVIDENCE ACQUISITION: The complexity of the blood plasma was first revealed by the Nobel Prize laureate, Arne Tiselius and Theodor Svedberg, which resulted in the identification of thousands of plasma proteins...
December 2016: Iranian Journal of Biotechnology
Rashid Saif, Ali Raza Awan, Leslie Lyons, Barbara Gandolfi, Muhammad Tayyab, Masroor Ellahi Babar, Asim Khalid Mehmood, Zia Ullah, Muhammad Wasim
BACKGROUND: Molecular marker based cancer diagnosis gaining more attention in the current genomics era. So, Hspb1 and Tp53 gene characterization and their mRNA expression might be helpful in diagnosis and prognosis of cat mammary adenocarcinoma. It will also add information in comparative cancer genetics and genomics. OBJECTIVES: Eight tumors of Siamese cats were analyzed to ascertain germ-line and tissue-specific somatic DNA variations of Hspb1 and Tp53 genes along with the ectopic differential expression in tumorous and normal tissues were also analyzed...
September 2016: Iranian Journal of Biotechnology
Hamida Bouridane, Mohamed Sifour, Tayeb Idoui, Lejeune Annick, Philip Thonard
BACKGROUND: For biotechnological application, selected lactic acid bacteria strains belonging to the genera Lactobacillus (Lb) are proposed as an alternative to the antibiotics for the prevention and treatment of urogenital tract infections. OBJECTIVES: Isolating and selecting vaginal lactobacilli strains for probiotic use based on their technological and probiotic aptitudes. MATERIALS AND METHODS: The vaginal isolates were examined for their essential characteristics as the potential probiotic such as low pH tolerance, bile-salt and simulated human intestinal fluid (SIF) resistance, adhesion to the vaginal epithelial cells (VECs), aggregation and coaggregation, surface hydrophobicity, antimicrobial activity, acid production, antibiotic resistance, and resistance to spermicides...
September 2016: Iranian Journal of Biotechnology
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