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Iranian Journal of Biotechnology

Lamia Medouni-Haroune, Farid Zaidi, Sonia Medouni-Adrar, Sevastianos Roussos, Samia Azzouz, Véronique Desseaux, Mouloud Kecha
BACKGROUND: Olive pomace, as the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production. OBJECTIVES: Actinobacteria isolates were separated from an Algerian soil under olive pomace cultivation and were evaluated for their lignocellulolytic enzymes production. MATERIALS AND METHODS: Isolates of Actinobacteria were separated from soils around oil mills using four isolation media, among them three were enriched by olive pomace...
March 2017: Iranian Journal of Biotechnology
Arezoo Tavakoli, Ainon Hamzah
BACKGROUND: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. OBJECTIVES: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized. MATERIALS AND METHODS: The xylC was amplified and cloned in E. coli. The recombinant plasmid pGEMT-xylC was digested by NdeI and HindIII to construct plasmid pET28b-xylC and transformed in E...
March 2017: Iranian Journal of Biotechnology
Bahareh Attaran, Tahereh Falsafi
BACKGROUND: A few reports confirm the ability of Helicobacter pylori to form biofilm. However, conclusive data do not exist concerning the factors that favor this ability. OBJECTIVES: Evaluation of the factors associated with the biofilm formation ability of H. pylori including bacterial, physical and chemical, and environmental factors was the research's aim. MATERIALS AND METHODS: H. pylori isolates from gastric biopsy specimens of patients infected chronically were screened for biofilm formation ability...
March 2017: Iranian Journal of Biotechnology
Kourosh Maboudi, Seyedeh Marzieh Hosseini, Mina Sepahi, Hashem Yaghoubi, Shahin Hadadian
BACKGROUND: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require specifi c antibodies. High cost, inappropriate shipping (cold chain failures), reduced sensitivity and thus poor detection performance are common pitfalls of using commercial kits for performing immunological tests...
March 2017: Iranian Journal of Biotechnology
Niloofar Zandvakili, Mohammadreza Zamani, Mostafa Motallebi, Zahra Moghaddassi Jahromi
BACKGROUND: Plants have various defense mechanisms such as production of antimicrobial peptides, particularly pathogenesis related proteins (PR proteins). PR10 family is an essential member of this group, with antifungal, antibacterial and antiviral activities. OBJECTIVE: The goal of this study is to assess the antifungal activity of maize PR10 against some of fungal phytopathogens. MATERIALS AND METHODS: Zea mays PR10 gene (TN-05-147) was cloned from genomic DNA and cDNA and overexpressed in Escherichia coli...
March 2017: Iranian Journal of Biotechnology
Habib Rezapoor, Mahnaz Aghdasi, Hamid Reza Sadeghipoor
BACKGROUND: TRR14 protein is a small protein, a member of a multigene family in Arabidopsis which was found as the fi rst protein during screening seedlings for their resistant to the trehalose sugar. OBJECTIVES: A number of TRR14-overexpressing plants were subjected to the characterization in the present research, among which, the associated morphological features and changes accompany growth pattern and photosynthesis related parameters. MATERIALS AND METHODS: TRR14 gene was isolated from Arabidopsis Thaliana and cloned into the pBin-35S vector...
March 2017: Iranian Journal of Biotechnology
Behzad Hajieghrari, Naser Farrokhi, Bahram Goliaei, Kaveh Kavousi
BACKGROUND: Micro RNAs (miRNAs) are a pivotal part of non-protein-coding endogenous small RNA molecules that regulate the genes involved in plant growth and development, and respond to biotic and abiotic environmental stresses posttranscriptionally. OBJECTIVE: In the present study, we report the results of a systemic search for identification of new miRNAs in B. rapa using homology-based ESTs (Expressed Sequence Tags) analysis and considering a series of fi ltration criteria...
March 2017: Iranian Journal of Biotechnology
Zahra Zamanzadeh, Mitra Ataei, Seyed Massood Nabavi, Ghasem Ahangari, Mehdi Sadeghi, Mohammad Hosein Sanati
BACKGROUND: Multiple sclerosis (MS) is the most common autoimmune disease of the central nervous system (CNS). The main cause of the MS is yet to be revealed, but the most probable theory is based on the molecular mimicry that concludes some infections in the activation of T cells against brain auto-antigens that initiate the disease cascade. OBJECTIVES: The Purpose of this research is the prediction of the auto-antigen potency of the myelin proteolipid protein (PLP) in multiple sclerosis...
March 2017: Iranian Journal of Biotechnology
Lin Hua, Hong Xia, Wei-Ying Zheng, Li An
BACKGROUND: Transforming growth factor (TGF)-β is over-expressed in a wide variety of cancers such as lung adenocarcinoma. TGF-β plays a major role in cancer progression through regulating cancer cell proliferation and remodeling of the tumor micro-environment. However, it is still a great challenge to explain the phenotypic effects caused by TGF-β stimulation and the effect of TGF-β stimulation on tumor micro-environment. OBJECTIVES: To address this issue, in the present study we used two time-course microarray data in human lung adenocarcinoma cells and applied bioinformatics methods to explore the gene regulation network responding to TGF-β stimulation in lung adenocarcinoma cells...
March 2017: Iranian Journal of Biotechnology
Seyyed Hossein Vafaei, Maryam Mahmoodi
Background: Cucurbit aphid-borne yellows virus (CABYV) is among the most important yellowing viruses on cucurbits in Iran. The presence of CABYV has been previously reported from the major cucurbit growing areas in Iran, however, there are few studies concerning the detection of the different strains of this virus in the grower's fields, and especially, there is no report of the weed host plants near crop fields. Objectives: This study was done in order to detect the new strains of the CABYV polerovirus in cucurbits and the weed plants in the Lorestan province, Iran, as an introductory investigation for initiating a program of the breeding for resistance...
2017: Iranian Journal of Biotechnology
Javad Karimi, Sasan Mohsenzadeh, Ali Niazi, Ali Moghadam
Background: The increasing use of nanoparticles (NPs) may have negative impacts on both organisms and the environment. Objectives: The differential expression of mitochondrial manganese superoxide dismutase ( MnSOD ) gene in wheat in response to silver nitrate nanoparticles (AgNPs) and AgNO3 was investigated. Materials and Methods: A quantitative Real-Time RT-PCR experiment was carried out with MnSOD gene using RNAs isolated from wheat shoots treated for 0, 2, 6, 12, and 24 h with 100 mg.L-1 of either AgNO 3 or AgNPs...
2017: Iranian Journal of Biotechnology
Ravi Theaj Prakash Upputuri, Abul Kalam Azad Mandal
Background: Green tea polyphenols (GTP) are known to have several health benefits. In spite of these benefits, its application as a therapeutic agent is limited due to some of its limitations such as stability, bioavailability, and biotransformation. To overcome these limitations, liposomal nanoparticles have been used as a carrier of the GTP. Objective: Encapsulation of GTP to the liposomal nanoparticles in order to achieve a sustained release of the GTP and to determine the drug release kinetics and the mechanism of the release...
2017: Iranian Journal of Biotechnology
Mojtaba Shakibaie, Mahboubeh Adeli-Sardou, Tayebe Mohammadi-Khorsand, Mahdie ZeydabadiNejad, Ehsan Amirafzali, Sahar Amirpour-Rostami, Atefeh Ameri, Hamid Forootanfar
Background: Recent theranostic (therapeutic or diagnostic) applications of tellurium nanoparticles have attracted a great interest for development of different methods for synthesis of this valuable nanostructure, especially via biological resources. Objectives: In the present study, the antimicrobial and antioxidant effects of the tellurium nanorods (Te NRs) biosynthesized by a bacterial strain Pseudomonas pseudoalcaligenes strain Te were evaluated. Materials and Methods: The antimicrobial effect of Te NRs and potassium tellurite against different bacterial and fungal pathogens was assessed by microdilution method...
2017: Iranian Journal of Biotechnology
Sajed Ali, Idrees Ahmad Nasir, Muhammad Rafiq, Shahid Javed Butt, Farooq Ihsan, Abdul Qayyum Rao, Tayyab Husnain
Background: Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives: pBINTRA6 vector was modifi ed by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1) and the two nonstructural proteins of TRV2(RNA2). Materials and Methods : SCMV construct was inoculated into 3-4 weeks Nicotiana benthamiana plant leaves either by using a needleless syringe or applying pricking with a toothpick...
2017: Iranian Journal of Biotechnology
Shakina Yesmin Simu, Sungeun Ahn, Veronica Castro-Aceituno, Deok-Chun Yang
Background: Obesity, a global health problem and a chronic disease, is associated with increased risk of developing type 2 diabetes and coronary heart diseases. A wide variety of natural remedies have been explored for their obesity treatment potential. Objective: The anti-adipogenic effect of ginsenoside Rg5:Rk1 (Rg5:Rk1) on 3T3-L1 mature adipocytes was investigated. Materials and Methods: To elucidate the anti-obesity effect of Rg5:Rk1, a mixture of protopanaxadiol type ginsenosides isolated from Panax ginseng Meyer in a 3T3-L1 adipocytes...
2017: Iranian Journal of Biotechnology
Farid Ebnerasuly, Zahra Hajebrahimi, Seyed Mehdi Tabaie, Mojtaba Darbouy
Background: Mesenchymal stem cells (MSCs) are multipotent cells able to differentiating into a variety of mesenchymal tissues including osteoblasts, adipocytes and several other tissues. Objectives: Differentiation of MSCs into fibroblast cells in vitro is an attractive strategy to achieve fibroblast cell and use them for purposes such as regeneration medicine. The goal of this study was investigate the simulated microgravity effect on differentiation of Adipose Derived Stem Cells (ADSCs) to fibroblasts. Materials and Methods: To fibroblast differentiation 100 ng...
2017: Iranian Journal of Biotechnology
Karuppasamy Geetha, Paramasamy Gunasekaran
Background: The need for more cost-effective compounds is imperative because the demand for prebiotic compounds is ever on the rise. Objective: The focus of this study is the purification of the endoxylanase from Bacillus pumilus B20 and its application in a cost-effective production of the prebiotic xylooligosaccharide (XOS) syrup having a high concentration of oligosaccharides. Materials and Methods: The extracellular endoxylanase was purified using ammonium sulphate fractionation, DEAE anion exchange, and Sephacryl gel filtration chromatography...
2017: Iranian Journal of Biotechnology
Tina Tavasoli, Sareh Arjmand, Seyed Omid Ranaei Siadat, Seyed Abbas Shojaosadati, Abbas Sahebghadam Lotfi
Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the potent inhibitor of a whole range of serine proteases and protects tissues against their destructive effects. The human plasma-derived AAT, which is currently used to augment the AAT level in patients, is limited due to high cost and source limitation. Recombinant production of AAT can be considered as a potential alternative. Objectives: This study aims to develop and optimize a new chemically defi ned medium based on an elemental analysis of the yeast Pichia pastoris for an effi cient culture of the recombinant yeast-producing secretory AAT...
2017: Iranian Journal of Biotechnology
Naeem Ali, Maira Anam, Sameen Yousaf, Sehrish Maleeha, Zain Bangash
Background: Different concentrations of the simple carbon substrates i.e. glucose, fructose, and sucrose were tested to enhance the performance of the mediator-less double chamber microbial fuel cell (MFC). Objectives: The power generation potential of the different electron donors was studied using a mesophilic Fe (III) reducer and non-fermentative bacteria Pseudomonas aeruginosa -isolated from municipal wastewater. Materials and Methods: A double chamber MFC was operated with three different electron donors including glucose, sucrose, and fructose...
2017: Iranian Journal of Biotechnology
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