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ACS Biomaterials Science & Engineering

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https://www.readbyqxmd.com/read/29333491/multifunctional-bioreactor-system-for-human-intestine-tissues
#1
Wenda Zhou, Ying Chen, Terrence Roh, Yinan Lin, Shengjie Ling, Siwei Zhao, James D Lin, Noor Khalil, Dana M Cairns, Eleana Manousiouthakis, Megan Tse, David L Kaplan
The three-dimensional (3D) cultivation of intestinal cells and tissues in dynamic bioreactor systems to represent in vivo intestinal microenvironments is essential for developing regenerative medicine treatments for intestinal diseases. We have previously developed in vitro human intestinal tissue systems using a 3D porous silk scaffold system with intestinal architectures and topographical features for the adhesion, growth, and differentiation of intestinal cells under static culture conditions. In this study, we designed and fabricated a multifunctional bioreactor system that incorporates pre-epithelialized 3D silk scaffolds in a dynamic culture environment for in vitro engineering of human intestine tissues...
January 8, 2018: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29333490/inhibition-of-bacterial-adhesion-on-nanotextured-stainless-steel-316l-by-electrochemical-etching
#2
Yeongseon Jang, Won Tae Choi, Christopher T Johnson, Andrés J García, Preet M Singh, Victor Breedveld, Dennis W Hess, Julie A Champion
Bacterial adhesion to stainless steel 316L (SS316L), which is an alloy typically used in many medical devices and food processing equipment, can cause serious infections along with substantial healthcare costs. This work demonstrates that nanotextured SS316L surfaces produced by electrochemical etching effectively inhibit bacterial adhesion of both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, but exhibit cytocompatibility and no toxicity toward mammalian cells in vitro. Additionally, the electrochemical surface modification on SS316L results in formation of superior passive layer at the surface, improving corrosion resistance...
January 8, 2018: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29302619/protein-mimetic-and-anticancer-properties-of-monocyte-targeting-peptide-amphiphile-micelles
#3
Christopher Poon, Sampreeti Chowdhuri, Cheng-Hsiang Kuo, Yun Fang, Francis J Alenghat, Danielle Hyatt, Kian Kani, Mitchell E Gross, Eun Ji Chung
Monocyte chemoattractant protein-1 (MCP-1) stimulates the migration of monocytes to inflammatory sites, leading to the progression of many diseases. Recently, we described a monocyte-targeting peptide amphiphile micelle (MCP-1 PAM) incorporated with the chemokine receptor CCR2 binding motif of MCP-1, which has a high affinity for monocytes in atherosclerotic plaques. We further report here the biomimetic components of MCP-1 PAMs and the influence of the nanoparticle upon binding to monocytes. We report that MCP-1 PAMs have enhanced secondary structure compared to the MCP-1 peptide...
December 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29285521/enhanced-differentiation-of-human-preosteoblasts-on-electrospun-blend-fiber-mats-of-polydioxanone-and-anionic-sulfated-polysaccharides
#4
Nowsheen Goonoo, Archana Bhaw-Luximon, Ulrich Jonas, Dhanjay Jhurry, Holger Schönherr
The viability and differentiation of SaOS-2 preosteoblasts on fiber mats of blends comprising of the biodegradable poly(ester-ether) polydioxanone (PDX) and the sulfate-containing anionic polysaccharides kappa-carrageenan (KCG) and fucoidan (FUC) were investigated for a range of different blend compositions. The detailed analysis of the blend nanofiber properties revealed a different degree of miscibility of PDX and the polysaccharide leading to a different enrichment at the surface of the blend nanofibers, which were observed to be stable in phosphate buffer solution (PBS) for up to 5 weeks...
December 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29250595/comparison-of-nmda-and-ampa-channel-expression-and-function-between-embryonic-and-adult-neurons-utilizing-microelectrode-array-systems
#5
Darin Edwards, Frank Sommerhage, Bonnie Berry, Hanna Nummer, Martina Raquet, Brad Clymer, Maria Stancescu, James J Hickman
Microelectrode arrays (MEAs) are innovative tools used to perform electrophysiological experiments for the study of electrical activity and connectivity in populations of neurons from dissociated cultures. Reliance upon neurons derived from embryonic tissue is a common limitation of neuronal/MEA hybrid systems and perhaps of neuroscience research in general, and the use of adult neurons could model fully functional in vivo parameters more closely. Spontaneous network activity was concurrently recorded from both embryonic and adult rat neurons cultured on MEAs for up to 10 weeks in vitro to characterize the synaptic connections between cell types...
December 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29250594/biomaterial-property-effects-on-platelets-and-macrophages-an-in-vitro-study
#6
Kelly R Fernandes, Yang Zhang, Angela M P Magri, Ana C M Renno, Jeroen J J P van den Beucken
The purpose of this study was to evaluate the effects of surface properties of bone implants coated with hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) on platelets and macrophages upon implant installation and compare them to grit-blasted Ti and Thermanox used as a control. Surface properties were characterized using scanning electron microscopy, profilometry, crystallography, Fourier transform infrared spectroscopy, and coating stability. For platelets, platelet adherence and morphology were assessed...
December 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29250593/methods-to-assess-shear-thinning-hydrogels-for-application-as-injectable-biomaterials
#7
Minna H Chen, Leo L Wang, Jennifer J Chung, Young-Hun Kim, Pavan Atluri, Jason A Burdick
Injectable hydrogels have gained popularity as a vehicle for the delivery of cells, growth factors, and other molecules to localize and improve their retention at the injection site, as well as for the mechanical bulking of tissues. However, there are many factors, such as viscosity, storage and loss moduli, and injection force, to consider when evaluating hydrogels for such applications. There are now numerous tools that can be used to quantitatively assess these factors, including for shear-thinning hydrogels because their properties change under mechanical load...
December 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29276759/in-vivo-multiscale-and-spatially-dependent-biomechanics-reveals-differential-strain-transfer-hierarchy-in-skeletal-muscle
#8
Soham Ghosh, James G Cimino, Adrienne K Scott, Frederick W Damen, Evan H Phillips, Alexander I Veress, Corey P Neu, Craig J Goergen
Biological tissues have a complex hierarchical architecture that spans organ to subcellular scales and comprises interconnected biophysical and biochemical machinery. Mechanotransduction, gene regulation, gene protection, and structure-function relationships in tissues depend on how force and strain are modulated from macro to micro scales, and vice versa. Traditionally, computational and experimental techniques have been used in common model systems (e.g., embryos) and simple strain measures were applied. But the hierarchical transfer of mechanical parameters like strain in mammalian systems is largely unexplored in vivo...
November 13, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29250592/actin-cytoskeleton-mediated-constriction-of-membrane-organelles-via-endoplasmic-reticulum-scaffolding
#9
Carol Lynn Curchoe, Uri Manor
Intracellular organelles constantly undergo fission to facilitate turnover, transport, and functional changes. The cytoskeleton has long been understood to play a role in these events, and recent work strongly suggests that several conserved molecular players cooperate with the cytoskeleton to mediate the fission process. Membrane curvature-inducing, membrane scission proteins, and force-inducing cytoskeletal proteins all cooperate to drive the fission process. Recent work suggests that the endoplasmic reticulum serves as the linchpin that orchestrates and spatially organizes fission via these curvature-inducing, scission, and force-producing molecules...
November 13, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29152561/targeted-modulation-of-tropoelastin-structure-and-assembly
#10
Giselle C Yeo, Clair Baldock, Steven G Wise, Anthony S Weiss
Tropoelastin, as the monomer unit of elastin, assembles into elastic fibers that impart strength and resilience to elastic tissues. Tropoelastin is also widely used to manufacture versatile materials with specific mechanical and biological properties. The assembly of tropoelastin into elastic fibers or biomaterials is crucially influenced by key submolecular regions and specific residues within these domains. In this work, we identify the functional contributions of two rarely occurring negatively charged residues, glutamate 345 in domain 19 and glutamate 414 in domain 21, in jointly maintaining the native conformation of the tropoelastin hinge, bridge and foot regions...
November 13, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29152560/effects-of-inflammation-on-multiscale-biomechanical-properties-of-cartilaginous-cells-and-tissues
#11
REVIEW
Q T Nguyen, T D Jacobsen, N O Chahine
Cells within cartilaginous tissues are mechanosensitive and thus require mechanical loading for regulation of tissue homeostasis and metabolism. Mechanical loading plays critical roles in cell differentiation, proliferation, biosynthesis, and homeostasis. Inflammation is an important event occurring during multiple processes, such as aging, injury, and disease. Inflammation has significant effects on biological processes as well as mechanical function of cells and tissues. These effects are highly dependent on cell/tissue type, timing, and magnitude...
November 13, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29147681/crimped-nanofibrous-biomaterials-mimic-microstructure-and-mechanics-of-native-tissue-and-alter-strain-transfer-to-cells
#12
Spencer E Szczesny, Tristan P Driscoll, Hsiao-Yun Tseng, Pang-Ching Liu, Su-Jin Heo, Robert L Mauck, Pen-Hsiu G Chao
To fully recapitulate tissue microstructure and mechanics, fiber crimping must exist within biomaterials used for tendon/ligament engineering. Existing crimped nanofibrous scaffolds produced via electrospinning are dense materials that prevent cellular infiltration into the scaffold interior. In this study, we used a sacrificial fiber population to increase the scaffold porosity and evaluated the effect on fiber crimping. We found that increasing scaffold porosity increased fiber crimping and ensured that the fibers properly uncrimped as the scaffolds were stretched by minimizing fiber-fiber interactions...
November 13, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28944287/detection-of-pentosidine-cross-links-in-cell-secreted-decellularized-matrices-using-time-resolved-fluorescence-spectroscopy
#13
Debika Mitra, Hussain Fatakdawala, Michael Nguyen-Truong, Amy Creecy, Jeffry Nyman, Laura Marcu, J Kent Leach
Hyperglycemia-mediated, nonenzymatic collagen cross-links such as pentosidine (PENT) can have deleterious effects on cellular interactions with the extracellular matrix (ECM). Present techniques to quantify PENT are limited, motivating the need for improved methods to study the accumulation and contribution of PENT toward diabetic clinical challenges such as impaired bone healing. Current methods for studying PENT are destructive, laborious, and frequently employ oversimplified collagen films that lack the complexity of the native ECM...
September 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28920077/mimicking-the-bioactivity-of-fibroblast-growth-factor-2-using-supramolecular-nanoribbons
#14
Charles M Rubert Pérez, Zaida Álvarez, Feng Chen, Taner Aytun, Samuel I Stupp
Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease...
September 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29152562/fast-dissolving-dendrimer-nanofiber-mats-as-alternative-to-eye-drops-for-more-efficient-antiglaucoma-drug-delivery
#15
Michael G Lancina, Sudha Singh, Uday B Kompella, Shahid Husain, Hu Yang
Polyamidoamine (PAMAM) dendrimers have been investigated as a potential platform for a number of ocular drugs, but only in aqueous solution. In this work we have developed fast dissolving dendrimer-based nanofibers (DNF) as a topical delivery vehicle for the glaucoma drug brimonidine tartrate (BT). The safety and drug release kinetics of these nanofiber mats were evaluated in vitro and in vivo. DNF caused no toxicity at therapeutic levels in cultured cells or ocular irritation in animal tests using a normotensive rat model...
August 14, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29147682/synthesis-and-application-of-injectable-bioorthogonal-dendrimer-hydrogels-for-local-drug-delivery
#16
Leyuan Xu, Remy C Cooper, Juan Wang, W Andrew Yeudall, Hu Yang
We developed novel dendrimer hydrogels (DH)s on the basis of bioorthogonal chemistry, in which polyamidoamine (PAMAM) dendrimer generation 4.0 (G4) functionalized with strained alkyne dibenzocyclooctyne (DBCO) via PEG spacer (Mn = 2,000 g/mol) underwent strain-promoted azide-alkyne cycloaddition (SPAAC) with polyethylene glycol bisazide (PEG-BA) (Mn= 20,000 g/mol) to generate a dendrimer-PEG cross-linked network. This platform offers a high degree of functionality and modularity. A wide range of structural parameters including dendrimer generation, degree of PEGylation, loading density of clickable DBCO groups, PEG-BA chain length as well as the ratio of clickable dendrimer to PEG-BA and their concentrations can be readily manipulated to tune chemical and physical properties of DHs...
August 14, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28989958/engineering-xeno-free-microcarriers-with-recombinant-vitronectin-albumin-and-uv-irradiation-for-human-pluripotent-stem-cell-bioprocessing
#17
Yongjia Fan, Fan Zhang, Emmanuel S Tzanakakis
The development of platforms for the expansion and directed differentiation of human pluripotent stem cells (hPSCs) in large quantities under xeno-free conditions is a key step toward the realization of envisioned stem cell-based therapies. Microcarrier bioreactors afford great surface-to-volume ratio, scalability and customization with typical densities of 10(6)-10(7) cells/ml or higher. In this study, a simple and inexpensive method was established for generating microcarriers without animal-derived components...
August 14, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28966980/predicting-silk-fiber-mechanical-properties-through-multiscale-simulation-and-protein-design
#18
Nae-Gyune Rim, Erin G Roberts, Davoud Ebrahimi, Nina Dinjaski, Matthew M Jacobsen, Zaira Martín-Moldes, Markus J Buehler, David L Kaplan, Joyce Y Wong
Silk is a promising material for biomedical applications, and much research is focused on how application-specific, mechanical properties of silk can be designed synthetically through proper amino acid sequences and processing parameters. This protocol describes an iterative process between research disciplines that combines simulation, genetic synthesis, and fiber analysis to better design silk fibers with specific mechanical properties. Computational methods are used to assess the protein polymer structure as it forms an interconnected fiber network through shearing and how this process affects fiber mechanical properties...
August 14, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28824959/predehydration-and-ice-seeding-in-the-presence-of-trehalose-enable-cell-cryopreservation
#19
Haishui Huang, Gang Zhao, Yuntian Zhang, Jiangsheng Xu, Thomas L Toth, Xiaoming He
Conventional approaches for cell cryopreservation require the use of toxic membrane-penetrating cryoprotective agents (pCPA), which limits the clinical application of cryopreserved cells. Here, we show intentionally induced ice formation at a high subzero temperature (> -10 °C) during cryopreservation, which is often referred to as ice seeding, could result in significant cell injury in the absence of any pCPA. This issue can be mitigated by predehydrating cells using extracellular trehalose to their minimal volume with minimized osmotically active water before ice seeding...
August 14, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28824958/confined-sandwichlike-microenvironments-tune-myogenic-differentiation
#20
José Ballester-Beltrán, Sara Trujillo, Enateri V Alakpa, Vicente Compañ, Rafael Gavara, Dominic Meek, Christopher C West, Bruno Péault, Matthew J Dalby, Manuel Salmerón-Sánchez
Sandwichlike (SW) cultures are engineered as a multilayer technology to simultaneously stimulate dorsal and ventral cell receptors, seeking to mimic cell adhesion in three-dimensional (3D) environments in a reductionist manner. The effect of this environment on cell differentiation was investigated for several cell types cultured in standard growth media, which promotes proliferation on two-dimensional (2D) surfaces and avoids any preferential differentiation. First, murine C2C12 myoblasts showed specific myogenic differentiation...
August 14, 2017: ACS Biomaterials Science & Engineering
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