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ACS Biomaterials Science & Engineering

E Huethorst, M Hortigon, V Zamora-Rodriguez, P M Reynolds, F Burton, G Smith, N Gadegaard
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) raise many possibilities for cardiac research but they exhibit an immature phenotype, which influences experimental outcomes. The aim of our research is to investigate the effects of a topographical gradient substrate on the morphology and function of commercially available hiPSC-CM. The lateral dimensions the microgrooves on the substrate varied from 8 to 100 μm space between the 8 μm grooves on one axis and from ∼5 nm to ∼1 μm in depth on the other axis...
December 12, 2016: ACS Biomaterials Science & Engineering
Tugba Ozdemir, Eric W Fowler, Shuang Liu, Daniel A Harrington, Robert L Witt, Mary C Farach-Carson, Swati Pradhan-Bhatt, Xinqiao Jia
Current treatments for chronic xerostomia, or "dry mouth", do not offer long-term therapeutic benefits for head and neck cancer survivors previously treated with curative radiation. Towards the goal of creating tissue-engineered constructs for the restoration of salivary gland functions, we developed new hyaluronic acid (HA)-based hydrogels using thiolated HA (HA-SH) and acrylated HA (HA-AES) with a significant molecular weight mismatch. Four hydrogel formulations with varying HA concentration, (1-2.4 wt%) and thiol/acrylate ratios (2/1 to 36/1) and elastic moduli (G': 35 to 1897 Pa, 2 h post-mixing) were investigated...
December 12, 2016: ACS Biomaterials Science & Engineering
Murat Guvendiren, Joseph Molde, Rosane M D Soares, Joachim Kohn
Three-dimensional (3D) printing is becoming an increasingly common technique to fabricate scaffolds and devices for tissue engineering applications. This is due to the potential of 3D printing to provide patient-specific designs, high structural complexity, rapid on-demand fabrication at a low-cost. One of the major bottlenecks that limits the widespread acceptance of 3D printing in biomanufacturing is the lack of diversity in "biomaterial inks". Printability of a biomaterial is determined by the printing technique...
October 10, 2016: ACS Biomaterials Science & Engineering
Ben P Hung, Bilal A Naved, Ethan L Nyberg, Miguel Dias, Christina A Holmes, Jennifer H Elisseeff, Amir H Dorafshar, Warren L Grayson
Tissue-engineered approaches to regenerate bone in the craniomaxillofacial region utilize biomaterial scaffolds to provide structural and biological cues to stem cells to stimulate osteogenic differentiation. Bioactive scaffolds are typically comprised of natural components but often lack the manufacturability of synthetic materials. To circumvent this trade-off, we 3D printed materials comprised of decellularized bone (DCB) matrix particles combined with polycaprolactone (PCL) to create novel hybrid DCB:PCL scaffolds for bone regeneration...
October 10, 2016: ACS Biomaterials Science & Engineering
Thomas J Hinton, Andrew Hudson, Kira Pusch, Andrew Lee, Adam W Feinberg
Polydimethylsiloxane (PDMS) elastomer is used in a wide range of biomaterial applications including microfluidics, cell culture substrates, flexible electronics, and medical devices. However, it has proved challenging to 3D print PDMS in complex structures due to its low elastic modulus and need for support during the printing process. Here we demonstrate the 3D printing of hydrophobic PDMS prepolymer resins within a hydrophilic Carbopol gel support via freeform reversible embedding (FRE). In the FRE printing process, the Carbopol support acts as a Bingham plastic that yields and fluidizes when the syringe tip of the 3D printer moves through it, but acts as a solid for the PDMS extruded within it...
October 10, 2016: ACS Biomaterials Science & Engineering
Hang Ren, Joseph L Bull, Mark E Meyerhoff
Nitric oxide (NO) releasing polymers are promising in improving the biocompatibility of medical devices. Polyurethanes are commonly used to prepare/fabricate many devices (e.g., catheters); however, the transport properties of NO within different polyurethanes are less studied, creating a gap in the rational design of new NO releasing devices involving polyurethane materials. Herein, we study the diffusion and partitioning of NO in different biomedical polyurethanes via the time-lag method. The diffusion of NO is positively correlated with the PDMS content within the polyurethanes, which can be rationalized by effective media theory considering various microphase morphologies...
September 12, 2016: ACS Biomaterials Science & Engineering
Woong Hee Lee, Hang Ren, Jianfeng Wu, Ondrej Novak, Richard B Brown, Chuanwu Xi, Mark E Meyerhoff
Herein, we report a novel design and the antimicrobial efficacy of a flexible nitric oxide (NO) releasing patch for potential wound healing applications. The compact sized polydimethylsiloxane (PDMS) planar patch generates NO via electrochemical reduction of nitrite ions mediated by a copper(II)-ligand catalyst using a portable power system and an internal gold coated stainless steel mesh working electrode. Patches are fabricated via soft lithography and 3-D printing. The devices can continuously release NO over 4 days and exhibit potent bactericidal effects on both Escherichia coli and Staphylococcus aureus...
September 12, 2016: ACS Biomaterials Science & Engineering
Eric Krueger, A Nicole Chang, Dale Brown, Josh Eixenberger, Raquel Brown, Sepideh Rastegar, Katie M Yocham, Kurtis D Cantley, David Estrada
This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on 3-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca(2+) fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 micrometers...
August 8, 2016: ACS Biomaterials Science & Engineering
Brittany N Eldridge, Brian W Bernish, Cale D Fahrenholtz, Ravi Singh
Glioblastoma multiforme (GBM) is the most common and most lethal primary brain tumor with a 5 year overall survival rate of approximately 5%. Currently, no therapy is curative and all have significant side effects. Focal thermal ablative therapies are being investigated as a new therapeutic approach. Such therapies can be enhanced using nanotechnology. Carbon nanotube mediated thermal therapy (CNMTT) uses lasers that emit near infrared radiation to excite carbon nanotubes (CNTs) localized to the tumor to generate heat needed for thermal ablation...
June 13, 2016: ACS Biomaterials Science & Engineering
Emily R Shearier, Patrick K Bowen, Weilue He, Adam Drelich, Jaroslaw Drelich, Jeremy Goldman, Feng Zhao
Zinc (Zn) and its alloys have recently been introduced as a new class of biodegradable metals with potential application in biodegradable vascular stents. Although an in vivo feasibility study pointed to outstanding biocompatibility of Zn-based implants in vascular environments, a thorough understanding of how Zn and Zn(2+) affect surrounding cells is lacking. In this comparative study, three vascular cell types-human endothelial cells (HAEC), human aortic smooth muscle cells (AoSMC), and human dermal fibroblasts (hDF)-were studied to advance the understanding of Zn/Zn(2+)-cell interactions...
April 11, 2016: ACS Biomaterials Science & Engineering
Christopher J Lowe, Ian M Reucroft, Matthew C Grota, David I Shreiber
Matrix and cellular alignment are critical factors in the native function of many tissues, including muscle, nerve, and ligaments. Collagen is frequently a component of these aligned tissues, and collagen biomaterials are widely used in tissue engineering applications. However, the generation of aligned collagen scaffolds that maintain the native architecture of collagen fibrils has not been straightforward, with many methods requiring specialized equipment or technical procedures, extensive incubation times, or denaturing of the collagen...
April 11, 2016: ACS Biomaterials Science & Engineering
John-Michael Williford, Maani M Archang, Il Minn, Yong Ren, Mark Wo, John Vandermark, Paul B Fisher, Martin G Pomper, Hai-Quan Mao
Nanoparticle-mediated gene delivery is a promising alternative to viral methods; however, its use in vivo, particularly following systemic injection, has suffered from poor delivery efficiency. Although PEGylation of nanoparticles has been successfully demonstrated as a strategy to enhance colloidal stability, its success in improving delivery efficiency has been limited, largely due to reduced cell binding and uptake, leading to poor transfection efficiency. Here we identified an optimized PEGylation scheme for DNA micellar nanoparticles that delivers balanced colloidal stability and transfection activity...
April 11, 2016: ACS Biomaterials Science & Engineering
Phin P Lee, Tejal A Desai
Current approaches to reducing restenosis do not balance the reduction of vascular smooth muscle cell proliferation with the increase in the healing of the endothelium. Building on our previous work, we present our study on the effects of Nitinol-based nanotubular coatings with different nanotube diameters on the reduction of restenosis. Here, we demonstrate that the nanotubular coatings reduced primary human aortic smooth muscle cell (HASMC) proliferation and increased the migration (by more than 4 times), collagen (by 2-3 times per cell) and elastin (by 5-8 times per cell) production of primary human aortic endothelial cells (HAEC)...
March 14, 2016: ACS Biomaterials Science & Engineering
Margaret Lashof-Sullivan, Mark Holland, Rebecca Groynom, Donald Campbell, Andrew Shoffstall, Erin Lavik
According to the CDC, the leading cause of death for both men and women between the ages of 5 and 44 is traumatic injury. Blood loss is the primary cause of death at acute time points post trauma. Early intervention is critical to save lives, and yet there are no treatments to stop internal bleeding that can be deployed in the field. In this work, we developed hemostatic nanoparticles that are stable at high temperatures (50 °C for 7 days) and are still effective at stopping bleeding and improving survival over the one hour time period in a rat liver injury model...
March 14, 2016: ACS Biomaterials Science & Engineering
Robert van Lith, Xuesong Wang, Guillermo Ameer
Intimal hyperplasia (IH) is a type of scarring that involves complex pathophysiological responses of the vasculature to injury, including overproliferation and migration of vascular smooth muscle cells (VSMCs), adventitial fibroblasts, and the activation of macrophages. The objective of this research was to develop a biodegradable polymer with intrinsic properties that would combat the cellular processes that contribute to IH. Citric acid, 1,8-octanediol, and all-trans retinoic acid (atRA) were incorporated into a polyester network via a condensation reaction to form the thermoset poly(1,8-octamethylene-citrate-co-retinate) (POCR)...
February 8, 2016: ACS Biomaterials Science & Engineering
Shaili Sharma, Nelda Vazquez-Portalatin, Sarah Calve, Alyssa Panitch
Aggrecan, the major proteoglycan in cartilage, serves to protect cartilage tissue from damage and degradation during the progression of osteoarthritis (OA). In cartilage extracellular matrix (ECM) aggrecan exists in an aggregate composed of several aggrecan molecules that bind to a single filament of hyaluronan. Each molecule of aggrecan is composed of a protein core and glycosaminoglycan sides chains, the latter of which provides cartilage with the ability to retain water and resist compressive loads. During the progression of OA, loss of aggrecan is considered to occur first, after which other cartilage matrix components become extremely susceptible to degradation...
February 8, 2016: ACS Biomaterials Science & Engineering
Namrata Raman, Karen Marchillo, Myung-Ryul Lee, Angélica de L Rodríguez López, David R Andes, Sean P Palecek, David M Lynn
Candida albicans is the most prevalent cause of hospital-acquired fungal infections and forms biofilms on indwelling medical devices that are notoriously difficult to treat or remove. We recently demonstrated that the colonization of C. albicans on the surfaces of catheter tube segments can be reduced in vitro by coating them with polyelectrolyte multilayers (PEMs) that release a potent antifungal β-peptide. Here, we report on the impact of polymer structure and film composition on both the inherent and β-peptide-mediated ability of PEM-coated catheters to prevent or reduce the formation of C...
January 11, 2016: ACS Biomaterials Science & Engineering
Zachary P Tolstyka, Haley Phillips, Mallory Cortez, Yaoying Wu, Nilesh Ingle, Jason B Bell, Perry B Hackett, Theresa M Reineke
The development and thorough characterization of nonviral delivery agents for nucleic acid and genome editing therapies are of high interest to the field of nanomedicine. Indeed, this vehicle class offers the ability to tune chemical architecture/biological activity and readily package nucleic acids of various sizes and morphologies for a variety of applications. Herein, we present the synthesis and characterization of a class of trehalose-based block copolycations designed to stabilize polyplex formulations for lyophilization and in vivo administration...
January 11, 2016: ACS Biomaterials Science & Engineering
Yu-Chieh Chiu, Joshua M Gammon, James I Andorko, Lisa H Tostanoski, Christopher M Jewell
New vaccine adjuvants that direct immune cells toward specific fates could support more potent and selective options for diseases spanning infection to cancer. However, the empirical nature of vaccines and the complexity of many formulations has hindered design of well-defined and easily characterized vaccines. We hypothesized that nanostructured capsules assembled entirely from polyionic immune signals might support a platform for simple, modular vaccines. These immune-polyelectrolyte (iPEM) capsules offer a high signal density, selectively expand T cells in mice, and drive functional responses during tumor challenge...
December 14, 2015: ACS Biomaterials Science & Engineering
Amy M Wen, Nga Le, Xin Zhou, Nicole F Steinmetz, Daniel L Popkin
Chronic viral infections (e.g., HIV, HBV, HCV) represent a significant source of morbidity and mortality with over 500 million people infected worldwide. Dendritic cells (DCs) and macrophages are key cell types for productive viral replication and persistent systemic infection. We demonstrate that the plant virus cowpea mosaic virus (CPMV) displays tropism for such antigen presenting cells in both mice and humans, thus making it an ideal candidate for targeted drug delivery toward viral infections. Furthermore, we show inhibition of a key host protein for viral infection, site-1 protease (S1P), using the small molecule PF-429242 in the model pathogen arenavirus lymphocytic choriomeningitis virus (LCMV) limits viral growth...
November 9, 2015: ACS Biomaterials Science & Engineering
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