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ACS Biomaterials Science & Engineering

Rongquan Duan, Davide Barbieri, Florence de Groot, Joost D de Bruijn, Huipin Yuan
Tricalcium phosphate (TCP) ceramics are used as bone void fillers because of their bioactivity and resorbability, while their performance in bone regeneration and material resorption vary with their physical properties (e.g., the dimension of the crystal grain). Herein, three TCP ceramic bone substitutes (TCP-S, TCP-M, and TCP-L) with gradient crystal grain size (0.77 ± 0.21 μm for TCP-S, 1.21 ± 0.35 μm for TCP-M and 4.87 ± 1.90 μm for TCP-L), were evaluated in a well-established rabbit lateral condylar defect model (validated with sham) with respect to bone formation and material resorption up to 26 weeks...
September 10, 2018: ACS Biomaterials Science & Engineering
Marije Sloff, Heinz P Janke, Paul K J D de Jonge, Dorien M Tiemessen, Barbara B M Kortmann, Silvia M Mihaila, Paul J Geutjes, Wout F J Feitz, Egbert Oosterwijk
Clinical implementation of novel products for tissue engineering and regenerative medicine requires a validated sterilization method. In this study, we investigated the effect of γ-irradiation and EtO degassing on material characteristics in vitro and the effect on template remodeling of hybrid tubular constructs in a large animal model. Hybrid tubular templates were prepared from type I collagen and Vicryl polymers and sterilized by 25 kGray of γ-irradiation or EtO degassing. The in vitro characteristics were extensively studied, including tensile strength analysis and degradation studies...
September 10, 2018: ACS Biomaterials Science & Engineering
Dongfei Liu, Katriina Lipponen, Peng Quan, Xiaocao Wan, Hongbo Zhang, Ermei Mäkilä, Jarno Salonen, Risto Kostiainen, Jouni Hirvonen, Tapio Kotiaho, Hélder A Santos
By exploiting its porous structure and high loading capacity, porous silicon (PSi) is a promising biomaterial to fabricate protocells and biomimetic reactors. Here, we have evaluated the impact of physicochemical properties of PSi particles [thermally oxidized PSi, TOPSi; annealed TOPSi, AnnTOPSi; (3-aminopropyl) triethoxysilane functionalized thermally carbonized PSi, APTES-TCPSi; and thermally hydrocarbonized PSi, THCPSi] on their surface interactions with different phospholipids. All of the four phospholipids were similarly adsorbed by the surface of PSi particles, except for TOPSi...
July 9, 2018: ACS Biomaterials Science & Engineering
Tania T Emi, Tanner Barnes, Emma Orton, Anne Reisch, Anita E Tolouei, S Zahra M Madani, Stephen M Kennedy
Pulsatile chemotherapeutic delivery profiles may provide a number advantages by maximizing the anticancer toxicity of chemotherapeutics, reducing off-target side effects, and combating adaptive resistance. While these temporally dynamic deliveries have shown some promise, they have yet to be clinically deployed from implantable hydrogels, whose localized deliveries could further enhance therapeutic outcomes. Here, several pulsatile chemotherapeutic delivery profiles were tested on melanoma cell survival in vitro and compared to constant (flatline) delivery profiles of the same integrated dose...
July 9, 2018: ACS Biomaterials Science & Engineering
Tadas Kasputis, Daniel Clough, Fallon Noto, Kevin Rychel, Briana Dye, Lonnie D Shea
Type I diabetes mellitus, which affects an estimated 1.5 million Americans, is caused by autoimmune destruction of the pancreatic beta cells that results in the need for life-long insulin therapy. Allogeneic islet transplantation for the treatment of type I diabetes is a therapy in which donor islets are infused intrahepatically, which has led to the transient reversal of diabetes. However, therapeutic limitations of allogeneic transplantation, which include a shortage of donor islets, long-term immunosuppression, and high risk of tissue rejection, have led to the investigation of embryonic or induced pluripotent stem cells as an unlimited source of functional beta-cells...
May 14, 2018: ACS Biomaterials Science & Engineering
Jekaterina Kazantseva, Roman Ivanov, Michael Gasik, Toomas Neuman, Irina Hussainova
Three-dimensional (3D) customized scaffolds are anticipated to provide new frontiers in cell manipulation and advanced therapy methods. Here, we demonstrate the application of hybrid 3D porous scaffolds, representing networks of highly aligned self-assembled ceramic nanofibers, for culturing four types of cancer cells. Ultrahigh aspect ratio (∼107 ) of graphene augmented fibers of tailored nanotopology is shown as an alternative tool to substantially affect cancerous gene expression, eventually due to differences in local biomechanical features of the cell-matrix interactions...
May 14, 2018: ACS Biomaterials Science & Engineering
Mohammed Al-Jarsha, Vladimíra Moulisová, Aldo Leal-Egaña, Andrew Connell, Kurt B Naudi, Ashraf F Ayoub, Matthew J Dalby, Manuel Salmerón-Sánchez
The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level...
May 14, 2018: ACS Biomaterials Science & Engineering
Kristin P O'Grady, Taylor E Kavanaugh, Hongsik Cho, Hanrong Ye, Mukesh K Gupta, Megan C Madonna, Jinjoo Lee, Christine M O'Brien, Melissa C Skala, Karen A Hasty, Craig L Duvall
The inherent antioxidant function of poly(propylene sulfide) (PPS) microspheres (MS) was dissected for different reactive oxygen species (ROS), and therapeutic benefits of PPS-MS were explored in models of diabetic peripheral arterial disease (PAD) and mechanically induced post-traumatic osteoarthritis (PTOA). PPS-MS (∼1 μ m diameter) significantly scavenged hydrogen peroxide (H2 O2 ), hypochlorite, and peroxynitrite but not superoxide in vitro in cell-free and cell-based assays. Elevated ROS levels (specifically H2 O2 ) were confirmed in both a mouse model of diabetic PAD and in a mouse model of PTOA, with greater than 5- and 2-fold increases in H2 O2 , respectively...
April 9, 2018: ACS Biomaterials Science & Engineering
Vimalkumar Balasubramanian, Andrea Poillucci, Alexandra Correia, Hongbo Zhang, Christian Celia, Hélder A Santos
Organelles of eukaryotic cells are structures made up of membranes, which carry out a majority of functions necessary for the surviving of the cell itself. Organelles also differentiate the prokaryotic and eukaryotic cells, and are arranged to form different compartments guaranteeing the activities for which eukaryotic cells are programmed. Cell membranes, containing organelles, are isolated from cancer cells and erythrocytes and used to form biocompatible and long-circulating ghost nanoparticles delivering payloads or catalyzing enzymatic reactions as nanoreactors...
April 9, 2018: ACS Biomaterials Science & Engineering
J Kent Leach, Jacklyn Whitehead
Cell-based therapies are a promising alternative to grafts and organ transplantation for treating tissue loss or damage due to trauma, malfunction, or disease. Over the past two decades, mesenchymal stem cells (MSCs) have attracted much attention as a potential cell population for use in regenerative medicine. While the proliferative capacity and multilineage potential of MSCs provide an opportunity to generate clinically relevant numbers of transplantable cells, their use in tissue regenerative applications has met with relatively limited success to date apart from secreting paracrine-acting factors to modulate the defect microenvironment...
April 9, 2018: ACS Biomaterials Science & Engineering
Baskaran Ganesh Kumar, Rustamzhon Melikov, Mohammad Mohammadi Aria, Aybike Ural Yalcin, Efe Begar, Sadra Sadeghi, Kaan Guven, Sedat Nizamoglu
Lithography, the transfer of patterns to a film or substrate, is the basis by which many modern technological devices and components are produced. However, established lithographic approaches generally use complex techniques, expensive equipment, and advanced materials. Here, we introduce a water-based microcontact printing method using silk that is simple, inexpensive, ecofriendly, and recyclable. Whereas the traditional microcontact printing technique facilitates only negative lithography, the synergetic interaction of the silk, water, and common chemicals in our technique enables both positive and negative patterning using a single stamp...
April 9, 2018: ACS Biomaterials Science & Engineering
Molly E Ogle, Jack R Krieger, Liane E Tellier, Jennifer McFaline-Figueroa, Johnna S Temenoff, Edward A Botchwey
The immune response to biomaterial implants critically regulates functional outcomes such as vascularization, transplant integration/survival, and fibrosis. To create "immunologically smart" materials, the host-material response may be engineered to optimize the recruitment of pro-regenerative leukocyte subsets which mature into corresponding wound-healing macrophages. We have recently identified a unique feature of pro-regenerative Ly6Clow monocytes that is a higher expression of both the bioactive lipid receptor sphingosine-1-phosphate receptor 3 (S1PR3) and the stromal derived factor-1α (SDF-1α) receptor CXCR4...
April 9, 2018: ACS Biomaterials Science & Engineering
Mehrdad T Kiani, Claire A Higgins, Benjamin D Almquist
The hair follicle is one of only two structures within the adult body that selectively degenerates and regenerates, making it an intriguing organ to study and use for regenerative medicine. Hair follicles have been shown to influence wound healing, angiogenesis, neurogenesis, and harbor distinct populations of stem cells; this has led to cells from the follicle being used in clinical trials for tendinosis and chronic ulcers. In addition, keratin produced by the follicle in the form of a hair fiber provides an abundant source of biomaterials for regenerative medicine...
April 9, 2018: ACS Biomaterials Science & Engineering
Lisa A Sawicki, Leila H Choe, Katherine L Wiley, Kelvin H Lee, April M Kloxin
Cells interact with and remodel their microenvironment, degrading large extracellular matrix (ECM) proteins (e.g., fibronectin, collagens) and secreting new ECM proteins and small soluble factors (e.g., growth factors, cytokines). Synthetic mimics of the ECM have been developed as controlled cell culture platforms for use in both fundamental and applied studies. However, how cells broadly remodel these initially well-defined matrices remains poorly understood and difficult to probe. In this work, we have established methods for widely examining both large and small proteins that are secreted by cells within synthetic matrices...
March 12, 2018: ACS Biomaterials Science & Engineering
Maryam Daviran, Hugo S Caram, Kelly M Schultz
Human mesenchymal stem cells (hMSCs) are encapsulated in synthetic matrix metalloproteinase (MMP) degradable poly(ethylene glycol)-peptide hydrogels to characterize cell-mediated degradation of the pericellular region using multiple particle tracking microrheology. The hydrogel scaffold is degraded by cell-secreted enzymes and cytoskeletal tension. We determine that cell-secreted enzymatic degradation is the main contributor to changes in the pericellular region, with cytoskeletal tension playing a minimal role...
February 12, 2018: ACS Biomaterials Science & Engineering
Carolyn R Shurer, Marshall J Colville, Vivek K Gupta, Shelby E Head, FuiBoon Kai, Jonathon N Lakins, Matthew J Paszek
The glycocalyx is a coating of protein and sugar on the surface of all living cells. Dramatic perturbations to the composition and structure of the glycocalyx are frequently observed in aggressive cancers. However, tools to experimentally mimic and model the cancer-specific glycocalyx remain limited. Here, we develop a genetically encoded toolkit to engineer the chemical and physical structure of the cellular glycocalyx. By manipulating the glycocalyx structure, we are able to switch the adhesive state of cells from strongly adherent to fully detached...
February 12, 2018: ACS Biomaterials Science & Engineering
Anna M Sitarski, Heather Fairfield, Carolyne Falank, Michaela R Reagan
Biological models are necessary tools for gaining insight into underlying mechanisms governing complex pathologies such as cancer in the bone. Models range from in vitro tissue culture systems to in vivo models and can be used with corresponding epidemiological and clinical data to understand disease etiology, progression, driver mutations, and signaling pathways. In bone cancer, as with many other cancers, in vivo models are often too complex to study specific cell-cell interactions or protein roles, and 2D models are often too simple to accurately represent disease processes...
February 12, 2018: ACS Biomaterials Science & Engineering
Shengqiang Xu, Seyedmehdi Hossaini Nasr, Daoyang Chen, Xiaoxian Zhang, Liangliang Sun, Xuefei Huang, Chunqi Qian
MicroRNA (miRNA) in urine has been considered as a potential biomarker for early-stage diagnosis of multiple diseases like urinary system cancer, kidney injury and diabetes, owing to their many demonstrated advantages including long-term stability and noninvasiveness. However, the traditional enrichment and extraction processes of miRNAs from urine are cumbersome and tedious due to the low concentration and multiple carriers of miRNAs. Herein, we present a novel method to collect low concentrations of miRNAs from dilute solutions such as urine and cell culture medium...
February 12, 2018: ACS Biomaterials Science & Engineering
Maria F Gencoglu, Lauren E Barney, Christopher L Hall, Elizabeth A Brooks, Alyssa D Schwartz, Daniel C Corbett, Kelly R Stevens, Shelly R Peyton
Improved in vitro models are needed to better understand cancer progression and bridge the gap between in vitro proof-of-concept studies, in vivo validation, and clinical application. Multicellular tumor spheroids (MCTS) are a popular method for three-dimensional (3D) cell culture, because they capture some aspects of the dimensionality, cell-cell contact, and cell-matrix interactions seen in vivo. Many approaches exist to create MCTS from cell lines, and they have been used to study tumor cell invasion, growth, and how cells respond to drugs in physiologically relevant 3D microenvironments...
February 12, 2018: ACS Biomaterials Science & Engineering
Andrea Malandrino, Roger D Kamm, Emad Moeendarbary
In addition to a multitude of genetic and biochemical alterations, abnormal morphological, structural, and mechanical changes in cells and their extracellular environment are key features of tumor invasion and metastasis. Furthermore, it is now evident that mechanical cues alongside biochemical signals contribute to critical steps of cancer initiation, progression, and spread. Despite its importance, it is very challenging to study mechanics of different steps of metastasis in the clinic or even in animal models...
February 12, 2018: ACS Biomaterials Science & Engineering
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