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International Journal of Bacteriology

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https://www.readbyqxmd.com/read/27819023/genotyping-of-mycobacterium-tuberculosis-isolates-from-hormozgan-province-of-iran-based-on-15-locus-miru-vntr-and-spoligotyping
#1
Samin Zamani, Mehri Haeili, Mohammad Javad Nasiri, Abbas Ali Imani Fooladi, Sedigheh Javadpour, Mohammad Mehdi Feizabadi
Background. Considering that Hormozgan province in Iran (southern part of Iran on the Persian Gulf) is among the areas with high prevalence of MDR-MTB and attracts so many sailors and tourists, genetic diversity of MTB isolates circulating in this part of Iran was evaluated. Pattern of TB transmission was also examined. Methods and Material. A total of 38 isolates of MTB were cultured from TB patients from Hormozgan province of Iran and standard MIRU-VNTR typing and spoligotyping were applied to genotype these isolates...
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/27660816/isolation-identification-and-antibiotic-susceptibility-testing-of-salmonella-from-slaughtered-bovines-and-ovines-in-addis-ababa-abattoir-enterprise-ethiopia-a-cross-sectional-study
#2
Abe Kebede, Jelalu Kemal, Haile Alemayehu, Solomon Habte Mariam
Salmonellae are ubiquitous, found in animals, humans, and the environment, a condition which facilitates transmission and cross contamination. Salmonella enterica serotypes exert huge health and economic impacts due to their virulence or carriage of antibiotic resistance traits. To address this significant issues with regard to public health, availability of adequate information on the prevalence and antibiotic resistance patterns of Salmonella, and establishment of adequate measures to control contamination and infection are needed...
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/27433480/methicillin-resistant-staphylococcus-aureus-recovered-from-healthcare-and-community-associated-infections-in-egypt
#3
Mohamed Abdel-Maksoud, Mona El-Shokry, Ghada Ismail, Soad Hafez, Amani El-Kholy, Ehab Attia, Maha Talaat
Background. Methicillin-resistant Staphylococcus aureus (MRSA) has created significant epidemiological, infection-control, and therapeutic management challenges during the past three decades. Aim. To analyze the pattern of resistance of healthcare- and community-associated MRSA in Egypt and the trend of resistance of HA-MRSA over time (2005-2013). Methods. MRSA isolates were recovered from healthcare-associated (HA) and community-associated (CA) Staphylococcus aureus (S. aureus) infections. They were tested against 11 antimicrobial discs and the minimal inhibitory concentration (MIC) of vancomycin was determined...
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/27299154/occurrence-of-potential-bacterial-pathogens-and-their-antimicrobial-susceptibility-patterns-isolated-from-herbal-medicinal-products-sold-in-different-markets-of-gondar-town-northwest-ethiopia
#4
Abdela Yesuf, Yitayih Wondimeneh, Teklay Gebrecherkos, Feleke Moges
Background. The World Health Organization estimates that about 80% of the world's population uses herbal medicine to treat various illnesses as means of primary healthcare. However, during preparation, herbal plants may be exposed to contamination by potential pathogens, and this may lead to infections. The aim of this study was to determine bacterial contamination of herbal medicinal products and to assess the antibiotic susceptibility pattern of the isolated bacteria. Methods. A cross-sectional study was conducted from January 1 to May 25, 2013, at Gondar Town...
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/27073807/direct-electrical-current-reduces-bacterial-and-yeast-biofilm-formation
#5
Maria Ruiz-Ruigomez, Jon Badiola, Suzannah M Schmidt-Malan, Kerryl Greenwood-Quaintance, Melissa J Karau, Cassandra L Brinkman, Jayawant N Mandrekar, Robin Patel
New strategies are needed for prevention of biofilm formation. We have previously shown that 24 hr of 2,000 µA of direct current (DC) reduces Staphylococcus epidermidis biofilm formation in vitro. Herein, we examined the effect of a lower amount of DC exposure on S. epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Propionibacterium acnes, and Candida albicans biofilm formation. 12 hr of 500 µA DC decreased S. epidermidis, S. aureus, E. coli, and P. aeruginosa biofilm formation on Teflon discs by 2, 1, 1, and 2 log10 cfu/cm(2), respectively (p < 0...
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/27042690/retracted-bacterial-biodegradation-of-crude-oil-using-local-isolates
#6
International Journal Of Bacteriology
[This retracts the article DOI: 10.1155/2014/863272.].
2016: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904750/review-on-the-antimicrobial-resistance-of-pathogens-from-tracheal-and-endotracheal-aspirates-of-patients-with-clinical-manifestations-of-pneumonia-in-bacolod-city-in-2013
#7
Alain C Juayang, Dominador G Maestral, Gemma B de Los Reyes, Michael Angelo D Acosido, Christine T Gallega
Microbiological content specifically bacterial and fungal etiologies from tracheal aspirates in a tertiary hospital in Bacolod City was reviewed for baseline information. A total of 130 tracheal aspirates were subjected for culture to isolate and identify the pathogen and determine their susceptibilities to various antibiotics. Productions of certain enzymes responsible for antibiotic resistance like ESBL (Extended Spectrum Beta-Lactamase), metallo-β-lactamase, and carbapenemase were also studied. Out of 130 specimens, 69...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904749/inhibition-of-quorum-sensing-controlled-virulence-factors-and-biofilm-formation-in-pseudomonas-aeruginosa-by-culture-extract-from-novel-bacterial-species-of-paenibacillus-using-a-rat-model-of-chronic-lung-infection
#8
Saad Musbah Alasil, Rahmat Omar, Salmah Ismail, Mohd Yasim Yusof
Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the culture extract of a taxonomically novel species of Paenibacillus strain 139SI for its inhibitory effects on the QS-controlled virulence factors and biofilm formation of Pseudomonas aeruginosa both in vitro and in vivo...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904748/role-of-burkholderia-pseudomallei-sigma-n2-in-amino-acids-utilization-and-in-regulation-of-catalase-e-expression-at-the-transcriptional-level
#9
Duong Thi Hong Diep, Nguyen Thi Thanh Phuong, Mya Myintzu Hlaing, Potjanee Srimanote, Sumalee Tungpradabkul
Burkholderia pseudomallei is the causative agent of melioidosis. The complete genome sequences of this pathogen have been revealed, which explain some pathogenic mechanisms. In various hostile conditions, for example, during nitrogen and amino acid starvation, bacteria can utilize alternative sigma factors such as RpoS and RpoN to modulate genes expression for their adaptation and survival. In this study, we demonstrate that mutagenesis of rpoN2, which lies on chromosome 2 of B. pseudomallei and encodes a homologue of the sigma factor RpoN, did not alter nitrogen and amino acid utilization of the bacterium...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904747/a-rapid-and-sensitive-diagnostic-screening-assay-for-detection-of-mycobacteria-including-mycobacterium-tuberculosis-directly-from-sputum-without-extraction
#10
Lisa Jane Cross, Catherine Anscombe, Timothy D McHugh, Ibrahim Abubakar, Robert John Shorten, Nicola Thorne, Cath Arnold
We report a novel approach utilising a real-time PCR screening assay targeting a 53 bp tandemly repeated element present at various loci within the Mycobacterium tuberculosis (Mtb) genome. Positive samples were identified within a discriminatory melting curve range of 90-94°C, with results obtained in under one hour directly from decontaminated sputum samples without extraction. A panel of 89 smear-positive sputa were used for analytical validation of the assay with 100% concordance, with sensitivity matching that of culture...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904746/bacterial-contamination-of-medical-doctors-and-students-white-coats-at-kilimanjaro-christian-medical-centre-moshi-tanzania
#11
Josephat Qaday, Margaretha Sariko, Adam Mwakyoma, Emmanuel Kifaro, Dominick Mosha, Richard Tarimo, Balthazar Nyombi, Elichilia Shao
Background. Microbial transmission from patient to patient has been linked to transient colonization of health care workers attires. Contamination of health care workers' clothing including white coats may play a big role in transmission of microbes. Study Objective. This study was conducted to determine the type of bacterial contamination on the white coats of medical doctors and students and associated factors. Methods. A cross-sectional study with purposive sampling of the bacterial contamination of white coats was undertaken...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904745/hyaluronidase-in-clinical-isolates-of-propionibacterium-acnes
#12
Harmony Tyner, Robin Patel
Objectives. We sought to describe the prevalence of a hyaluronidase gene and hyaluronidase production in 197 clinical isolates of P. acnes; we assessed kinetics of hyaluronidase production in a subset of three isolates. Methods. The hyaluronidase gene was detected using polymerase chain reaction. Hyaluronidase production was detected by growing isolates on BHI agar containing 400 μg/mL hyaluronic acid and 1% albumin and flooding plates with 2 N glacial acetic acid to precipitate unbound hyaluronic acid, with a zone of clearing representing a positive phenotype...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904744/antibacterial-effects-of-cissus-welwitschii-and-triumfetta-welwitschii-extracts-against-escherichia-coli-and-bacillus-cereus
#13
Batanai Moyo, Stanley Mukanganyama
Antibiotic resistance has increased sharply, while the pace for the development of new antimicrobials has slowed down. Plants provide an alternative source for new drugs. This study aimed to screen extracts from Cissus welwitschii and Triumfetta welwitschii for antibacterial activity against Escherichia coli and Bacillus cereus. The tests conducted included a susceptibility determination test, analysis of the effect of T. welwitschii on cell wall integrity, and transport across the membrane. It was found that the T...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904743/highly-sensitive-loop-mediated-isothermal-amplification-for-the-detection-of-leptospira
#14
Hua-Wei Chen, Giulia Weissenberger, Erin Atkins, Chien-Chung Chao, Yupin Suputtamongkol, Wei-Mei Ching
Leptospirosis is a worldwide zoonosis caused by an infection with the pathogenic species of Leptospira. We have developed a loop-mediated isothermal amplification (LAMP) assay to detect the DNA of Leptospira spp. Six sets of primers targeting the gene of the subsurface protein, lipL32, were evaluated for their detection sensitivity. The best primer set detected less than 25 copies of lipL32 per reaction of both plasmid DNA template and purified leptospiral genomic DNA. By combining primers targeting lipL32 with the previously published primer set targeting lipL41, the sensitivity of the assay was improved to 12 copies of L...
2015: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26962545/mix-infections-of-helicobacter-pylori-a-major-risk-factor-affecting-genotyping-studies
#15
Amin Talebi Bezmin Abadi
No abstract text is available yet for this article.
2014: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904742/bacterial-biodegradation-of-crude-oil-using-local-isolates
#16
Raed S Al-Wasify, Shimaa R Hamed
An experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At temperature 22°C, the mixed bacterial consortium degraded a maximum of 88...
2014: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904741/phylogenetic-framework-and-biosurfactant-gene-expression-analysis-of-marine-bacillus-spp-of-eastern-coastal-plain-of-tamil-nadu
#17
Sreethar Swaathy, Varadharajan Kavitha, Arockiasamy Sahaya Pravin, Ganesan Sekaran, Asit Baran Mandal, Arumugam Gnanamani
The present study emphasizes the diversity assessment of marine Bacillus species with special reference to biosurfactant production, respective gene expression, and discrimination among Bacillus licheniformis and Bacillus subtilis. Among the 200 individual species of eastern coastal plain of Tamil Nadu screened, five biosurfactant producing potential bacterial species with entirely different morphology were selected. Biochemical and 16S rRNA gene sequence analysis suggested that all the said five species belong to Bacillus genera but differ in species levels...
2014: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904740/antibiofilm-activity-of-manuka-honey-in-combination-with-antibiotics
#18
Michelle E M Campeau, Robin Patel
We assessed the in vitro activity of Manuka honey against biofilm bacteria in combination with antibiotics and visualized the effect of Manuka honey on bacterial biofilms using scanning electron microscopy. The fractional biofilm eradication concentration (∑FBEC) index for vancomycin plus Manuka honey against S. aureus IDRL-4284 biofilms was 0.34, indicating a synergistic interaction. The ∑FBEC index for gentamicin plus Manuka honey against P. aeruginosa PAO1 biofilms was 0.78-0.82, indicating an additive interaction...
2014: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904739/detection-of-q-fever-specific-antibodies-using-recombinant-antigen-in-elisa-with-peroxidase-based-signal-amplification
#19
Hua-Wei Chen, Zhiwen Zhang, Erin Glennon, Wei-Mei Ching
Currently, the accepted method for Q fever serodiagnosis is indirect immunofluorescent antibody assay (IFA) using the whole cell antigen. In this study, we prepared the recombinant antigen of the 27-kDa outer membrane protein (Com1) which has been shown to be recognized by Q fever patient sera. The performance of recombinant Com1 was evaluated in ELISA by IFA confirmed serum samples. Due to the low titers of IgG and IgM in Q fever patients, the standard ELISA signals were further amplified by using biotinylated anti-human IgG or IgM plus streptavidin-HRP polymer...
2014: International Journal of Bacteriology
https://www.readbyqxmd.com/read/26904738/the-frequency-of-methicillin-resistant-staphylococcus-aureus-and-coagulase-gene-polymorphism-in-egypt
#20
Hend M Abdulghany, Rasha M Khairy
The current study aimed to use Coagulase gene polymorphism to identify methicillin-resistant Staphylococcus aureus (MRSA) subtypes isolated from nasal carriers in Minia governorate, Egypt, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. A total of 400 specimens were collected from nasal carriers in Minia governorate, Egypt, between March 2012 and April 2013. Fifty-eight strains (14.5%) were isolated and identified by standard microbiological methods as MRSA...
2014: International Journal of Bacteriology
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