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Journal of Biological Research

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https://www.readbyqxmd.com/read/29568733/new-marine-gastropod-records-for-the-hellenic-waters
#1
Thanasis Manousis, Constantinos Kontadakis, Georgios Polyzoulis, George Mbazios, Sofia Galinou-Mitsoudi
Background: The Hellenic Seas are influenced by on-going environmental changes and the introduction of alien species, which are expected to have an impact on their biodiversity. This study contributes to the knowledge of the Hellenic marine gastropod biodiversity, expanding data over the entire Greek territory, during the period from October 2008 to March 2017. Results: This work presents 45 species of gastropods not previously reported from Greece or reported only once, belonging to 19 families...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29556481/cadmium-uptake-kinetics-in-parts-of-the-seagrass-cymodocea-nodosa-at-high-exposure-concentrations
#2
Paraskevi Malea, Theodoros Kevrekidis, Konstantina-Roxani Chatzipanagiotou, Athanasios Mogias
Background: Seagrass species have been recommended as biomonitors of environmental condition and as tools for phytoremediation, due to their ability to concentrate anthropogenic chemicals. This study aims to provide novel information on metal accumulation in seagrasses under laboratory conditions to support their use as a tool in the evaluation and abatement of contamination in the field. We investigated the kinetics of cadmium uptake into adult leaf blades, leaf sheaths, rhizomes and roots of Cymodocea nodosa in exposure concentrations within the range of cadmium levels in industrial wastewater (0...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29456971/molecular-biochemical-and-kinetic-analysis-of-a-novel-thermostable-lipase-lipsm-from-stenotrophomonas-maltophilia-psi-1-the-first-member-of-a-new-bacterial-lipase-family-xviii
#3
Maria Parapouli, Athanasios Foukis, Panagiota-Yiolanda Stergiou, Maria Koukouritaki, Panagiotis Magklaras, Olga A Gkini, Emmanuel M Papamichael, Amalia-Sofia Afendra, Efstathios Hatziloukas
Background: Microbial lipases catalyze a broad spectrum of reactions and are enzymes of considerable biotechnological interest. The focus of this study was the isolation of new lipase genes, intending to discover novel lipases whose products bear interesting biochemical and structural features and may have a potential to act as valuable biocatalysts in industrial applications. Results: A novel lipase gene ( lipSm ), from a new environmental Stenotrophomonas maltophilia strain, Psi-1, originating from a sludge sample from Psittaleia (Greece), was cloned and sequenced...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29445623/bone-marrow-stem-cells-to-destroy-circulating-hiv-a-hypothetical-therapeutic-strategy
#4
Umesh Chandra Halder
Human immunodeficiency virus (HIV) still poses enigmatic threats to human life. This virus has mastered in bypassing anti retroviral therapy leading to patients' death. Circulating viruses are phenomenal for the disease outcome. This hypothesis proposes a therapeutic strategy utilizing receptor-integrated hematopoietic, erythroid and red blood cells. Here, HIV specific receptors trap circulating viruses that enter erythrocyte cytoplasm and form inactive integration complex. This model depicts easy, effective removal of circulating HIV without any adverse effect...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29404283/genetic-diversity-analysis-of-cultivated-korarima-aframomum-corrorima-braun-p-c-m-jansen-populations-from-southwestern-ethiopia-using-inter-simple-sequence-repeats-issr-marker
#5
Dagmawit Chombe, Endashaw Bekele
Background: Korarima (Aframomum corrorima) is a perennial and aromatic herb native and widely distributed in southwestern Ethiopia. It is known for its fine flavor as a spice in various Ethiopian traditional dishes. Few molecular studies have been performed on this species so far. In the present paper, the ISSR technique was employed to study the genetic diversity in populations of cultivated A. corrorima. Results: Seven ISSR primers produced a total of 86 clearly scorable DNA bands...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29299441/effects-of-partial-dietary-supplementation-of-fish-meal-with-soymeal-on-the-stress-and-apoptosis-response-in-the-digestive-system-of-common-dentex-dentex-dentex
#6
Efthimia Antonopoulou, Eleni Chouri, Konstantinos Feidantsis, Antigone Lazou, Stavros Chatzifotis
Background: Soybean is a common alternative protein source of plant origin in aquafeeds as it has a reasonably balanced amino acid profile and is widely available. This study aimed to investigate the influence of partial substitution of fish meal with soy meal on cytoprotective pathways and apoptosis in the digestive system of common dentex ( Dentex dentex ), using the activation of Hsp70, p38 MAPK, Bcl-2 and caspase-3. The experimental approach involved feeding of common dentex with three isoprotein and isoenergetic diets that contained fish meal as a protein source (FM), partial replacement of fish meal by soy meal 25% (SM25) and 40% (SM40) for 3 months...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/29299440/host-species-and-pathogenicity-effects-in-the-evolution-of-the-mitochondrial-genomes-of-eimeria-species-apicomplexa-coccidia-eimeriidae
#7
Asma Awadi
Background: Mitochondria are fundamental organelles responsible for cellular metabolism and energy production in eukaryotes via the oxidative phosphorylation pathway. Mitochondrial DNA is often used in population and species studies with the assumption of neutral evolution. However, evidence of positive selection in mitochondrial coding genes of various animal species has accumulated suggesting that amino acid changes in mtDNA might be adaptive. The functional and physiological implications of the inferred positively selected sites are usually unknown and are only evaluated based on available structural and functional models...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/29209596/aflp-reveals-low-genetic-diversity-of-the-bryozoan-pectinatella-magnifica-leidy-1851-in-the-czech-republic
#8
Vendula Moravcová, Jana Moravcová, Vladislav Čurn, Zuzana Balounová, Josef Rajchard, Lenka Havlíčková
Background: Non-native species have aroused scientific interest because of their ability to successfully colonise areas to which they have been introduced, despite their sometimes limited genetic variation compared to their native range. These species establish themselves with the aid of some pre-existing features favouring them in the new environment. Pectinatella magnifica (Leidy, 1851), the freshwater magnificent bryozoan, is non-native in Europe and Asia. This study was designed to determine the genetic diversity and population structure of P...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28975085/evaluation-of-a-simple-method-for-storage-of-blood-samples-that-enables-isolation-of-circulating-tumor-cells-96%C3%A2-h-after-sample-collection
#9
Panagiotis Apostolou, Dimitrios-Athanasios Ntanovasilis, Ioannis Papasotiriou
BACKGROUND: Minimizing the effects of transportation on the properties of biological material is a major challenge for the scientific community. The viability of cells is important in cases where their study is urgent for evaluation of treatment response or for the study of cancer progression. Circulating tumor cells (CTCs) constitute a cell subpopulation with great importance for oncologists, because of their prognostic value. Detection and isolation of CTCs from blood samples is a routine activity in many laboratories, but concerns exist with regard to the maintenance of the cells during transportation...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28795052/microrna-454-may-function-as-an-oncogene-via-targeting-akt-in-triple-negative-breast-cancer
#10
Qun Li, Jia Liu, Xianying Meng, Renzhu Pang, Jie Li
BACKGROUND: Altered microRNAs expression mediates tumor development and progression in many type cancers including triple negative breast cancer (TNBC). Here we detected the effect of miR-454 on cell proliferation, migration and invasion of triple negative breast cancer cells. RESULTS: miR-454 promoted the proliferation of TNBC, and enhanced migration and invasion in TNBC cells. Meanwhile, miR-454 improved the survival of TNBC cells after ironizing radiation. miR-454 inhibited radiation-induced apoptosis in TNBC cells by regulation of caspase 3/7 and Bcl-2 expression...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28523251/bone-marker-gene-expression-in-calvarial-bones-different-bone-microenvironments
#11
Osama Al-Amer
BACKGROUND: In calvarial mice, mesenchymal stem cells (MSCs) differentiate into osteoprogenitor cells and then differentiate into osteoblasts that differentiate into osteocytes, which become embedded within the bone matrix. In this case, the cells participating in bone formation include MSCs, osteoprogenitor cells, osteoblasts and osteocytes. The calvariae of C57BL/KaLwRijHsD mice consist of the following five bones: two frontal bones, two parietal bones and one interparietal bone. This study aimed to analyse some bone marker genes and bone related genes to determine whether these calvarial bones have different bone microenvironments...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28413783/two-single-nucleotide-polymorphisms-sites-in-%C3%AE-1-at-gene-and-their-association-with-somatic-cell-score-in-chinese-holstein-cows
#12
Xiao-Fei Guo, Wen-Ping Hu, Xian-Zheng Lang, Qiu-Ling Li, Xiang-Yu Wang, Ran Di, Qiu-Yue Liu, Xiao-Lin Liu, Yong-Fu An, Ming-Xing Chu
BACKGROUND: Alpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of α1-AT gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in α1-AT gene...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28405579/a-survey-of-methods-and-tools-to-detect-recent-and-strong-positive-selection
#13
REVIEW
Pavlos Pavlidis, Nikolaos Alachiotis
Positive selection occurs when an allele is favored by natural selection. The frequency of the favored allele increases in the population and due to genetic hitchhiking the neighboring linked variation diminishes, creating so-called selective sweeps. Detecting traces of positive selection in genomes is achieved by searching for signatures introduced by selective sweeps, such as regions of reduced variation, a specific shift of the site frequency spectrum, and particular LD patterns in the region. A variety of methods and tools can be used for detecting sweeps, ranging from simple implementations that compute summary statistics such as Tajima's D, to more advanced statistical approaches that use combinations of statistics, maximum likelihood, machine learning etc...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28316956/sudden-infant-death-syndrome-due-to-long-qt-syndrome-a-brief-review-of-the-genetic-substrate-and-prevalence
#14
REVIEW
Nikolaos S Ioakeimidis, Theodora Papamitsou, Soultana Meditskou, Zafiroula Iakovidou-Kritsi
The pathophysiological mechanisms which lead to sudden infant death syndrome (SIDS) are not completely understood. Cardiac channelopathies are a well-established causative factor with long QT syndrome (LQTS) being the most frequent one, accounting for approximately 12% of SIDS cases. The genetic substrate of the above arrhythmogenic syndrome has been thoroughly described but only specific gene mutations or polymorphisms have been identified as SIDS causative. The review will focus on the prevalence of LQTS-induced SIDS or near-SIDS cases and the mutations held responsible...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28265554/protective-role-of-hesperidin-against-%C3%AE-radiation-induced-oxidative-stress-and-apoptosis-in-rat-testis
#15
Nadia Z Shaban, Ahmed M Ahmed Zahran, Fatma H El-Rashidy, Ahmad S Abdo Kodous
BACKGROUND: Gamma (γ) ray, an electromagnetic radiation, is occasionally accompanying the emission of an alpha or beta particle. Exposure to such radiation can cause cellular changes such as mutations, chromosome aberration and cellular damage which depend upon the total amount of energy, duration of exposure and the dose. Ionizing radiation can impair spermatogenesis and can cause mutations in germ cells. In general, type B spermatogonia are sensitive to this type of radiation. The current study was carried out to evaluate the protective role of hesperidin (H), as a polyphenolic compound, on rat testis injury induced by γ-radiation...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28251115/the-aegean-archipelago-a-natural-laboratory-of-evolution-ecology-and-civilisations
#16
REVIEW
Spyros Sfenthourakis, Kostas A Triantis
The Aegean archipelago, comprising numerous islands and islets with great heterogeneity in topographic, geological, historical and environmental properties, offers an ideal natural laboratory for ecological and evolutionary research, and has been the stage for a very long interaction between human civilizations and local ecosystems. This work presents insights that have been gained from past and current relevant research in the area, highlighting also the importance of the Aegean archipelago as a useful model to address many major questions in biogeography, ecology and evolutionary processes...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28239596/genetic-diversity-of-atlantic-bluefin-tuna-in-the-mediterranean-sea-insights-from-genome-wide-snps-and-microsatellites
#17
Aglaia Antoniou, Panagiotis Kasapidis, Georgios Kotoulas, Constantinos C Mylonas, Antonios Magoulas
BACKGROUND: Elucidating the patterns of the Atlantic Bluefin tuna [ABFT, Thunnus thynnus (Linnaeus, 1758)] population structure constitutes a challenging task of great importance. Most of the unique challenges stem from its biology, as well as the attributes of the marine realm in which it disperses. Accurate information is urgently needed for stock assessment, and the identification of critical features to the persistence and adaptation of populations in order to formulate and adopt effective strategies for ABFT conservation and management...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28164041/evolution-and-inheritance-of-animal-mitochondrial-dna-rules-and-exceptions
#18
REVIEW
Emmanuel D Ladoukakis, Eleftherios Zouros
Mitochondrial DNA (mtDNA) has been studied intensely for "its own" merit. Its role for the function of the cell and the organism remains a fertile field, its origin and evolution is an indispensable part of the evolution of life and its interaction with the nuclear DNA is among the most important cases of genome synergism and co-evolution. Also, mtDNA was proven one of the most useful tools in population genetics and molecular phylogenetics. In this article we focus on animal mtDNA and discuss briefly how our views about its structure, function and transmission have changed, how these changes affect the information we have accumulated through its use in the fields of phylogeny and population structure and what are the most important questions that remain open for future research...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/28105407/campanula-lingulata-populations-on-mt-olympus-greece-where-s-the-abundant-centre
#19
Anastasia E Tzortzaki, Despoina Vokou, John M Halley
BACKGROUND: The abundant-centre hypothesis (ACH) assumes that a species becomes more abundant at the centre of its range, where the environmental conditions are most favorable. As we move away from this centre, abundance and occupancy decline. Although this is obvious intuitively, efforts to confirm the hypothesis have often failed. We investigated the abundance patterns of Campanula lingulata across its altitudinal range on Mt. Olympus, Greece, in order to evaluate the "abundant centre" hypothesis along an elevation gradient...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/27981039/effects-of-cell-seeding-density-on-real-time-monitoring-of-anti-proliferative-effects-of-transient-gene-silencing
#20
Cigdem Selli, Yasemin Erac, Metiner Tosun
BACKGROUND: Real-time cellular analysis systems enable impedance-based label-free and dynamic monitoring of various cellular events such as proliferation. In this study, we describe the effects of initial cell seeding density on the anti-proliferative effects of transient gene silencing monitored via real-time cellular analysis. We monitored the real-time changes in proliferation of Huh7 hepatocellular carcinoma and A7r5 vascular smooth muscle cells with different initial seeding densities following transient receptor potential canonical 1 (TRPC1) silencing using xCELLigence system...
December 2016: Journal of Biological Research
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