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Balsaeng'gwa Saengsig

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https://www.readbyqxmd.com/read/30023470/prenatally-diagnosed-rare-trisomy-16-mosaicism-in-human-amniotic-fluid-cells-in-the-second-trimester-a-case-report
#1
Sook Ryung Kim, Eun Jung Choi, Young Joo Kim, Tae Yoon Kim, Young Jin Lee
Although trisomy 16 is commonly detected in spontaneous abortions and accounts for over 30% of cases of autosomal trisomy detected after spontaneous abortion, trisomy 16 mosaicism is rarely detected by amniocentesis in the second trimester. Here, we report a case of level III trisomy 16 mosaicism (47,XX,+16[8]/46,XX[31]) diagnosed by cytogenetic analysis of independently cultured amniotic fluid cells. The female baby was delivered at full term with low birth weight and intrauterine growth retardation, and interestingly, her karyotype was normal (46,XX)...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023469/genetic-variations-of-intra-and-between-razor-clam-solen-corneus-population-identified-by-pcr-analysis
#2
Jong-Man Yoon
The author undertook PCR-founded genetic platform to investigate the hierarchical dendrogram of Euclidean genetic distances of one razor clam population, particularly for Solen corneus , which was further associated with those of the other clam population, by engaging with the precisely designed oligonucleotide primer sets. Seven oligonucleotides primers were used producing a total of 639 counted bands in population A and 595 in population B, respectively, ranging in size of DNA fragments from larger than approximately 50 bp to less than 1,100 bp...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023468/morphometric-characteristics-of-diploid-and-triploid-marine-medaka-oryzias-dancena
#3
In-Seok Park, Hyun Woo Gil, Dong Soo Kim
The morphometric truss characteristics and classical dimensions of the marine medaka, Oryzias dancena , that might distinguish diploid and triploid fish were examined. Significant differences in all the classical and truss dimensions of the diploid and triploid fish were observed in both sexes ( p <0.01). All the dimensions of the triploid fish were greater than those of the diploid fish. The triploid marine medaka shows sexual dimorphism in these characters, and the sexual dimorphism of the triploid marine medaka is similar to that of the diploid marine medaka...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023467/effects-of-different-light-spectra-on-the-oocyte-maturation-in-grass-puffer-takifugu-niphobles
#4
Song-Hee Choi, Byeong-Hoon Kim, Sung-Pyo Hur, Chi-Hoon Lee, Young-Don Lee
In order to examine the effects of four different light spectra (white, red, green, and blue) on the oocyte maturation, the change of reproductive parameters, via brain-pituitary-gonad (BPG) axis in grass puffer, were investigated. After exposure four different light spectra for 7 weeks, the abundance of gonadotropin-releasing hormone ( GnRH ) mRNA which is a type of seabream ( sbGnRH ) and two different subunit of gonadotropin hormones mRNAs, follicle-stimulating hormone ( fshβ ) mRNA and luteinizing hormone ( lhβ ) mRNA, were analyzed in the brain and pituitary...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023466/pysiological-responses-of-diploid-and-triploid-far-eastern-catfish-silurus-asotus-to-water-temperature-stress
#5
Hyo Bin Lee, Dong Soo Kim, Hyun Woo Gil, In-Seok Park
The aim of study is to contribute to this knowledge-base by investigating the respiratory function, the metabolic rate and the difference of physiological responses un-der low water temperature (20℃→15℃) stress be-tween diploid and triploid far eastern catfish, Silurus asotus . During the 48 hrs of water temperature stress exposure time, the respiratory frequencies, CO2 and NH4 + concentrations of diploid had higher values than those of triploid ( p <0.05). However, pH of triploid was higher than those of diploid ( p <0...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023465/effects-of-17%C3%AE-estradiol-interleukin-1%C3%AE-and-human-chorionic-gonadotropin-on-activity-and-mrna-expression-of-plasminogen-activators-in-porcine-endometrial-cells
#6
Yong Hwangbo, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
This study aimed to investigate changes in the activity and mRNA expression of plasminogen activators (PAs) induced by 17β-estradiol (E2 ), human chorionic gonadotropin (hCG), and interleukin-1β (IL-1β) in porcine endometrial cells. Endometrial cells were isolated from the epithelium and cultured to 80% confluence. They were then treated for 24 h with E2 (0.2, 2, 20, and 200 ng/mL), IL-1β (0.1, 1, 10, and 100 ng/mL), and hCG (0.5, 1, 1.5 and 2 IU/mL). mRNA expressions of urokinase-type (uPA) and tissue-type (tPA) PAs were analyzed using reverse transcription PCR, and activities were measured using a PA activity assay...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023464/the-c-terminal-phosphorylation-sites-of-eel-follicle-stimulating-hormone-receptor-are-important-role-in-the-signal-transduction
#7
Jeong-Min Kim, Munkhzaya Byambaragchaa, Myung-Hwa Kang, Kwan-Sik Min
The large extracellular domain of glycoprotein hormone receptors is a unique feature within the G protein-coupled receptors (GPCRs) family. After interaction with the hormone, the receptor becomes coupled to Gs, which, in turn stimulates adenylyl cyclase and the production of cAMP. Potential phosphorylation sites exist in the C-terminal region of GPCRs. The experiments described herein represent attempts to determine the functions of the eel follicle-stimulating hormone receptor (eelFSHR). We constructed a mutant of eelFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 614 (eelFSHR-t614)...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023463/combination-of-bez235-and-metformin-has-synergistic-effect-on-cell-viability-in-colorectal-cancer-cells
#8
Taewan Kim, Taehyung Kim, Soonyoung Choi, Hyeran Ko, Deokbae Park, Youngki Lee
Patients with type II diabetes mellitus are more susceptible to colorectal cancer (CRC) incidence than non-diabetics. The anti-diabetic drug metformin is most commonly prescribed for the treatment of this disease and has recently shown antitumor effect in preclinical studies. The aberrant mutational activation in the components of RAS/RAF/MEK/ERK and PI3K/AKT/mTOR signaling pathway is very frequently observed in CRC. We previously reported that metformin inhibits the phosphorylation of ERK and BEZ235, a dual inhibitor of PI3K and mTOR, has anti-tumor activity against HCT15 CRC cells harboring mutations of KRAS and PIK3CA...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/30023462/evolutionary-viewpoint-on-gnrh-gonadotropin-releasing-hormone-in-chordata-amino-acid-and-nucleic-acid-sequences
#9
REVIEW
Donchan Choi
GnRH (gonadotropin-releasing hormone) is a supreme hormone regulating reproductive activity in most animals. The sequences of amino acid and nucleic acid of GnRH reported up to now are examined from the evolutionary framework of Chordata. All identified GnRH are classified into GnRH1, GnRH2, or GnRH3. In all three forms of GnRH both N-terminal and C-terminal are conserved, which allows for effective binding to their receptors. The three amino acids in the middle of GnRH1 sequence have altered diversely from the primitive Chordata, which is indicative of the adaptation process to the ambient environment...
June 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707690/temperature-dependent-of-mitotic-interval-for-grass-puffer-takifugu-niphobles
#10
Min Gyun Ko, Hyo Bin Lee, Hyun Woo Gil, Shin Beom Kang, In-Seok Park, Dong Soo Kim
The objective of this study was to determine the mitotic intervals (τ0 ) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and 24℃). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was 0.09±0.002 mg. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at 20℃ ( p <0.05). The size of the fertilized egg of 18℃ water temperature group at the blastodisc stage was the smallest ( p <0...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707689/new-ambulatory-hysteroscopic-septoplasty-using-ballooning-in-a-woman-with-complete-septate-uterus-a-case-report
#11
Jung Hyun Cho, Hyung Jae Won, Mi Kyoung Kim, Ju Hee Park, Ju Youn Hwang
A 40-year-old G1 P0 L0 A1 woman was referred to our clinic with 6-year history of infertility. Before visiting the clinic, she had 3 cycles of In-Vitro Fertilization (IVF) procedures (2 cycles of Controlled Ovarian Stimulation-IVF and 1 cycle of frozen-thawed Embryo Transfer (ET)) at other clinic. She had medical history of abortion at early gestation following FET (frozen-thawed-ET). The patient had complete type of septate uterus, double cervix and longitudinal vaginal septum. Vaginal septotomy was done first and 1 month later, hysteroscopic septoplasty was followed using ballooning filled with dye...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707688/regulation-of-cyclic-amp-response-element-binding-protein-zhangfei-crebzf-expression-by-estrogen-in-mouse-uterus
#12
Hoon Jang
CREBZF (cAMP-response element binding protein zhangfei) is a member of ATF/CREB family, and which regulates various cellular functions by suppressing major factors with direct interaction. In this study, we have examined the expression of CREBZF on mouse endometrium during uterus estrous cycles and estrogen (E2) treatment. In uterus, CREBZF mRNA expression was higher than other organs and mRNA and protein of CREBZF was increased in proestrus phase and decreased in estrus phase. The expression of CREBZF in 3-weeks old mouse uterus was reduced by E2 injection in endometrium...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707687/nonmonotonic-effects-of-chronic-low-dose-di-2-ethylhexyl-phthalate-on-gonadal-weight-and-reproductive
#13
Sunyeong Cha, Kayeon Jung, Min Young Lee, Yeon Jeong Hwang, Eunhyeok Yang, Sung-Ho Lee, Hyo-Il Jung, Yong-Pil Cheon
Endocrine disruptors have been concerned in toxicology but now challenged as physiological point especially concerned with exposing dose and period. In this study the low-dose chronic administration of di(2-ethylhexyl) phthaltae (DEHP) during reproductive period was examined to evaluate the possible roles. Adult male and female CD-1 mice were exposed to DEHP with drinking water containing 133 1g/L and 1,330 /g/L DEHP in water according to OECD 433 guide line and sacrificed just after weaning. The weights of uterus and ovary were decreased by drinking of 1,330 /g/L DEHP water...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707686/analysis-of-endoplasmic-reticulum-er-stress-induced-during-somatic-cell-nuclear-transfer-scnt-process-in-porcine-scnt-embryos
#14
Hwa-Yeon Lee, Hyo-Kyung Bae, Bae-Dong Jung, Seunghyung Lee, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
This study investigates the endoplasmic reticulum (ER) stress and subsequent apoptosis in duced during somatic cell nuclear transfer (SCNT) process of porcine SCNT embryos. Porcine SCNT and in vitro fertilization (IVF) embryos were sampled at 3 h and 20 h after SCNT or IVF and at the blastocyst stage for mRNA extraction. The x-box binding protein 1 (Xbp1) mRNA and the expressions of ER stress-associated genes were confirmed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. Before commencing SCNT, somatic cells treated with tunicamycin (TM), an ER stress inducer, confirmed the splicing of Xbp1 mRNA and increased expressions of ER stress-associated genes...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707685/differential-expression-of-cyclic-amp-response-element-binding-protein-zhangfei-crebzf-in-the-mouse-testis-during-postnatal-development
#15
Hoon Jang
Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating transcription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707684/signal-transduction-of-eel-luteinizing-hormone-receptor-eellhr-and-follicle-stimulating-hormone-receptor-eelfshr-by-recombinant-equine-chorionic-gonadotropin-rec-ecg-and-native-ecg
#16
Munkhzaya Byambaragchaa, So-Yun Lee, Dae-Jung Kim, Myung-Hwa Kang, Kwan-Sik Min
Previous studies showed that recombinant equine chorionic gonadotropin (rec-eCGβ/α) exhibits both follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-like activities in rat LHR- and FSHR-expressing cells. In this study, we analyzed signal transduction by eelFSHR and eelLHR upon stimulation with rec-eCGβ/α and native eCG. The cyclic adenosine monophosphate (cAMP) stimulation in CHO-K1 cells expressing eelLHR was determined upon exposure to different doses (0-1,450 ng/mL) of rec-eCGβ/α and native eCG...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707683/early-life-history-of-rhodeus-fish-r-uyekii-and-r-ocellatus-in-the-nakdong-river-water-system
#17
Jae-Min Park, Kyeong-Ho Han
The purpose of this study is to investigate the early life history of the Rhodeus fish, Rhodeus uyekii and R. ocellatus , in the Nakdong River to use as the preliminary data for the systematic study. The embryos used in the study were fertilized eggs (embryo) and larvae after artificial fertilization. The long diameter of the eggs of the R. uyekii was 3.39-3.97 mm (average 3.68±0.41 mm, n=30) and the short diameter was 1.36-1.55 mm (average 1.45±0.13 mm, n=30). The long diameter of the eggs of the R. ocellatus was 2...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707682/expressional-patterns-of-connexin-isoforms-in-the-rat-epididymal-fat-during-postnatal-development
#18
Ki-Ho Lee, Nan Hee Kim
In the multicellular tissue, cell-cell interaction is important for a precise control of its function. The exchange of signaling molecules between adjacent cells via connexon allows the functional harmony of cells in the tissue. The present research was to determine the presence and expressional patterns of connexin ( Cx ) isoforms in the rat epididymal fat during postnatal development using quantitative real-time polymerase chain reaction (PCR) analysis. Of 13 Cx isoforms examined, expression of 11 Cx isoforms in the epididymal fat during postnatal development was detected...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707681/hershberger-assays-for-di-2-ethylhexyl-phthalate-and-its-substitute-candidates
#19
Hee-Su Kim, Yong-Pil Cheon, Sung-Ho Lee
In the present study, we employed Hershberger assay to determine possible androgenic or antiandrogenic activities of three di-2-ethylhexyl phthalate (DEHP) substitute candidates. The assay was carried out using immature castrated Sprague-Dawley male rats. After 7 days of the surgery, testosterone propionate (TP, 0.4 mg/kg/day) and test materials (low dose, 40 mg/kg/day; high dose, 400 mg/kg/day) were administered for 10 consecutive days by subcutaneous (s.c.) injection and oral gavage, respectively. Test materials were DEHP, 2-ethylhexyl oleate (IOO), 2-ethylhexyl stearate (IOS) and triethyl 2-acetylcitrate (ATEC)...
March 2018: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29707680/osteological-development-of-the-larvae-and-juvenile-of-bullhead-torrent-catfish-liobagrus-obesus
#20
Won-Il Seo, Jae-Min Park, Sung-Hun Lee, Seong Min Yoon, Seon-Yeong Hwang, Kyeong-Ho Han
This study was conducted to investigate the skeletal development of bullhead torrent catfish, Liobagrus obesus larvae and to utilize them as basic data for the taxonomic study of Liobagrus larvae. Skeletal development was observed by being divided into cranium, visceral skeleton, shoulder girdle bone, pelvic girdle bone and vertebra. On the first day after hatching, the pre-larvae had an average total length of 7.92 mm, and a line-shaped parasphenoid ossified in the cranium. In the jaw bone, the dentary supporting the lower jaw and the maxillary supporting the upper jaw were ossified...
March 2018: Balsaeng'gwa Saengsig
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