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Balsaeng'gwa Saengsig

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https://www.readbyqxmd.com/read/29082350/gene-editing-interpretation-of-current-law-and-legal-policy
#1
Na-Kyoung Kim
With the development of the third-generation gene scissors, CRISPR-Cas9, concerns are being raised about ethical and social repercussions of the new gene-editing technology. In this situation, this article explores the legislation and interpretation of the positive laws in South Korea. The BioAct does not specify and regulate 'gene editing' itself. However, assuming that genetic editing is used in the process of research and treatment, we can look to the specific details of the regulations for research on humans as well as gene therapy research in order to see how genetic editing is regulated under the BioAct...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082349/ell3-modulates-the-wound-healing-activity-of-conditioned-medium-of-adipose-derived-stem-cells
#2
Jae-Yong Lee, Nuri Oh, Kyung-Soon Park
While adipose-derived stem cell-conditioned medium (ADSC-CM) has been demonstrated to promote skin wound healing, the mechanism regulating this effect remains unelucidated. In this study, we aimed to investigate the role of Ell3 in the wound healing activity of ADSC-CM. In vitro analysis revealed that Ell3 suppression in ADSCs impairs the promotive activity of ADSC-CM on the proliferation and migration of mouse embryonic fibroblasts (MEF) and normal human dermal fibroblasts (NHDF). Consistently, the expression of MMP family genes, which regulate cell proliferation and migration, was significantly suppressed in MEF and NHDF treated with siEll3-transfected ADSC-CM...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082348/expression-of-luteinizing-hormone-lh-subunit-genes-in-mouse-testis
#3
Hee Soo Kim, Sung-Ho Lee
Gonadotropins are heterodimers consisting an alpha chain (Cgα) and a beta chain. Interestingly, presence of complicated LH-β transcripts in rat testis was accidently found; testicular LH-β transcripts were confined in seminiferous tubules to spermatids, and the translated products were localized in the elongated spermatids. We hypothesized that mouse testis has potential to produce the tissue specific LH-β with similar structure to the rat testicular forms. To verify our hypothesis, we examined the adult mouse (ICR) testis using RT-PCR and immunohistochemistry...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082347/expressional-changes-of-connexin-isoform-genes-in-the-rat-caput-epididymis-exposed-to-flutamide-or-estradiol-benzoate-at-the-early-postnatal-age
#4
Ki-Ho Lee
Direct communication between neighboring cells through connexin (Cx)-based gap junction is a crucial biolo-gical manner to regulate functions of a tissue consisting of multi-cell types. The present research evaluated expressional changes of Cx isoforms in the caput epididymis of adult rat exposed to estradiol benzoate (EB) or flutamide (Flu) at the early postnatal age. A single subcutaneous administration of EB at a low-dose [0.015 µg /kg body weight (BW)] or a high-dose (1.5 µg/kg BW) or Flu at a low-dose (500 µg/kg BW) or a high-dose (5 mg/kg BW) was performed to an animal at 1 week of age...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082346/evaluation-of-newly-developed-chemical-castration-method-changes-in-hormone-gene-expression-of-hypothalamic-pituitary-axis
#5
Byung Kuk Kwak, Sung-Ho Lee
Surgical castration (also known as orchidectomy, ORX) has been frequently performed to avoid uncontrolled breeding. However, it has some serious disadvantages. Several laboratories have developed chemical castration methods, using bilateral intratesticular injection (BITI) of simple chemical solutions. The present study was undertaken to compare the effects of ORX and of hypertonic saline BITI on the androgen-sensitive tissues such as pituitary and hypothalamus. Serum testosterone (T) levels of ORX animals and hypertonic saline BITI animals (SAL) after 4 weeks of the manipulations exhibited significantly drops as compared with the levels of intact animals (Intact:ORX:SAL = 7...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082345/annual-reproductive-cycle-of-acheilognathus-majusculus-a-korean-endemic-species
#6
Jin-Yeong Lim, Won-Kyo Lee
An experiment was conducted to investigate the annual reproductive cycle of a Korean endemic species, Acheilognathus majusculus, from Jeokseong-myeon located in Seomjin River. The reproductive cycle is examined histologically regarding water temperature and day length of the habitat, the gonadosomatic index (GSI), the female ovipositor length index (OLI), monthly variation in egg diameter distribution, and developmental characteristics of female and male gonads. The maximum GSI was found in 19.21±2.32 and 6...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082344/egg-development-and-larvae-and-juveniles-morphology-of-carp-cyprinus-carpio-in-korean
#7
Jae Min Park, Seong Jun Mun, Hu Sun Yim, Kyeong Ho Han
This study was conducted to observe egg and larvae morphological development of carp to obtain basic data for resource conservation and taxonomic research. Brood carp used in the research (total length 67.3-75.5 cm, average 71.0±3.45 cm) were bred in a circular rearing aquarium (600×300×100 cm) using a running water system from January to July, 2015. Breeding water temperature was maintained at 23.0-25.0℃(average 24.0℃). Fertilized carp eggs were translucent and globular, and their size was 1.75-1.89 mm (average 1...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082343/change-of-various-characteristics-between-spawning-and-non-spawning-season-in-diploid-and-induced-triploid-far-eastern-catfish-silurus-asotus
#8
Sun Young Lim, Hyun Woo Gil, In-Seok Park
The purpose of this study is to investigate the differences fatty acids and hormonal parameters in the spawning and non-spawning season between the diploid and induced triploid Far Eastern catfish, Silurus asotus. The measured triploids were produced by cold shock for 50 min at 4℃ in May 2014, the spawning season of diploid was in May, and the non-spawning season was designated in January. Estradiol and testosterone and gonadosomatic index of diploid were higher than those of induced triploid in spawning season (P<0...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082342/euclidean-genetic-distances-of-four-manila-clam-ruditapes-philippinarum-populations-analyzed-by-pcr-research
#9
Jong-Man Yoon
The PCR analysis was performed on DNA samples extracted from a total of 20 individuals using six oligonucleotides primers. The author accomplished clustering analyses to reveal the Euclidean genetic distances among four clam populations from Gochang, Seocheon, Taean and Anmyeon of the Korean peninsula. The oligonucleotides primer OPA-08 generated 5 unique loci to each population, approximately 550 bp and 600 bp, respectively, in the MCS population. Especially, the primer OPA-20 generated 15 unique loci to each population, which were identifying each population, approximately 400 bp, 750 bp and 800 bp, in the MCT population...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082341/anti-diabetic-effects-of-ethanol-extract-from-bitter-melon-in-mice-fed-a-high-fat-diet
#10
Nal Ae Yoon, Juyeong Park, Jiyeon Lee, Joo Yeon Jeong, Hyun-Kyu Kim, Hak Sung Lee, In Guk Hwang, Gu Seob Roh, Hyun Joon Kim, Gyeong Jae Cho, Wan Sung Choi, Dong Hoon Lee, Sang Soo Kang
Present study aimed to determine the effect of 'bitter melon', a popularly used fruit in Bangladesh and several other Asian countries, on high-fat-diet-induced type 2 diabetes. To investigate the effect, ethanol extract from bitter melon (BME) as a dietary supplement with mouse chow was used. BME was found to significantly attenuate the high-fat diet (HFD) -induced body weight and total fat mass. BME also effectively reduced the insulin resistance induced by the HFD. Furthermore, dietary supplementation of BME was highly effective in increasing insulin sensitivity and reducing hepatic fat and obesity...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082340/starvation-induced-physiological-responses-and-rna-dna-ratios-in-rock-bream-oplegnathus-fasciatus-and-olive-flounder-paralichthys-olivaceus
#11
In-Seok Park, Hyun Woo Gil, Bong-Seok Kim, Kwan-Ha Park, Sung-Yong Oh
In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values and those of the fed fish. The RNA contents and RNA/DNA ratios of the fed fish were significantly higher than those of the other groups (P<0.05). The hematocrit, hemoglobin, red blood cells (RBC), and mean corpuscular volume (MCV) of the fed rock bream were significantly higher than at baseline (P<0...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082339/dnajc18-a-novel-type-iii-dnaj-family-protein-is-expressed-specifically-in-rat-male-germ-cells
#12
Cynthia Gomes, Jaemog Soh
Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082338/a-simple-confocal-microscopy-based-method-for-assessing-sperm-movement
#13
Sung Woo Kim, Min Su Ki, Chan-Lan Kim, In-Sul Hwang, Ik Soo Jeon
In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28791335/internalization-of-rat-fsh-and-lh-cg-receptors-by-rec-ecg-in-cho-k1-cells
#14
Jong-Ju Park, Hun-Ki Seong, Jeong-Soo Kim, Byambaragchaa Munkhzaya, Myung-Hwa Kang, Kwan-Sik Min
Equine chorionic gonadotropin (eCG) is a unique molecule that elicits the response characteristics of both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in other species. Previous studies from this laboratory had demonstrated that recombinant eCG (rec-eCG) from Chinese hamster ovary (CHO-K1) cells exhibited both FSH- and LH-like activity in rat granulosa and Leydig cells. In this study, we analyzed receptor internalization through rec-eCGs, wild type eCG (eCGβ/α) and mutant eCG (eCGβ/αΔ56) with an N-linked oligosaccharide at Asn(56) of the α-subunit...
June 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28785743/effects-of-photoperiod-manipulation-on-gonadal-activity-of-the-damselfish-chromis-notata
#15
Chi-Hoon Lee, Young-Ju Park, Young-Don Lee
This study investigated the effect of different photoperiods (14L: 10D, 12L:12D and 10L:14D) on the gonadal development and GtH mRNA expression in the pituitary of damselfish. The results showed that gonadosomatic index (GSI) was significantly lower in shot photoperiod (10L:14D), in comparison with other photoperiodic group during the spawning season. After 60 days treatment, histological analysis of gonad tissue showed that the gonad of 10L:14D and 12L: 12D treatment groups were resting phase with spermatogonia and perinucleolus stage oocytes but the gonad of 14L:10D treatment group was still ripe phase with spermatozoa and mature stage oocyte...
June 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28785742/bitter-melon-seed-extract-does-not-alter-photoperiodic-effects-on-reproduction-of-male-golden-hamsters
#16
Donchan Choi, Su Ji Lee, Min Hyuck Lee, Dong Kyu Lee
Bitter melon (Momordica charantia, MC) has been used in traditional Korean medicine in treating diabetes. In addition, some reports were emerged, showing the antifertility activities of MC in mammals. We investigated the effects of ethanolic MC extract on the reproductive activity of golden hamsters whose spermatogenetic capacity is controlled by their photoperiods. The animals were divided into 4 groups: long photoperiod (LP) control, short photoperiod (SP) control, and LP animals treated with MC. The animals were orally ingested with low (0...
June 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28785741/effect-of-alpha-linolenic-acid-on-oocyte-maturation-and-embryo-development-in-pigs
#17
Ji-Eun Lee, Hwangbo Yong, Hwa-Young Kim, Won-Hee Lee, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
The aim of this study was to determine the effect of additional alpha-linolenic acid (ALA) supplementation during in vitro maturation (IVM) and culture (IVC) on nucleic maturation and embryo development of pigs. Cumulus-oocyte complexes (COCs) were incubated in IVM medium containing different concentration of ALA (25, 50 and 100 μM) for 44 h. After in vitro maturation, nuclear maturation of oocytes were evaluated by aceto-orcein stain. Mature oocytes with 50 μM ALA were fertilized and cultured in IVC medium with ALA (25, 50 and 100 μM) during early-embryogenesis (48 hours after fertilization)...
June 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28785740/comparative-analysis-of-tissue-and-cell-cycle-on-the-far-eastern-catfish-silurus-asotus-between-diploid-and-triploid
#18
Hyun Woo Gil, Tae Ho Lee, Ho Jae Han, In-Seok Park
The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue...
June 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28785739/various-characteristics-of-hybrid-between-river-puffer-takifugu-obscurus-and-tiger-puffer-t-rubripes-and-their-hybrid-triploid
#19
In-Seok Park, Sung Young Lim, Tae Ho Lee, Hyun Woo Gil, Gwang Yeol Yoo
A comparison of the growth, hematological values, fatty acids, and gonadal and growth hormonal changes of river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids (river puffer × tiger puffer) and hybrid triploids was performed during 3 months of their early growth period. Several features were observed during these 3 months: hybrids showed the highest levels of specific growth rate, 1.48%; hybrid triploids showed the smallest change in viscera fat (P<0.05), but GSI was not significantly different among groups (P>0...
June 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28785738/involvement-of-pi3k-and-mmp1-in-pdgf-induced-migration-of-human-adipose-derived-stem-cells
#20
Yoonhwa Lim, Minji Lee, Hyeju Jeong, Haekwon Kim
Human adult stem cells have widely been examined for their clinical application including their wound healing effect in vivo. To function as therapeutic cells, however, cells must represent the ability of directed migration in response to signals. This study aimed to investigate the mechanism of platelet-derived growth factor (PDGF)-induced migration of the human abdominal adipose-derived stem cells (hADSCs) in vitro. A general matrix metalloproteinase (MMP) inhibitor or a MMP2 inhibitor significantly inhibited the PDGF-induced migration...
June 2017: Balsaeng'gwa Saengsig
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