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Central-European Journal of Immunology

Halyna D Koval, Valentyna V Chopyak, Oleksandr M Kamyshnyi, Maciej K Kurpisz
Endometriosis is a disease of epidemiological gravity of unknown primary reason. A complex of constitutional factors including the immune system has been considered as its background. The aim of the study was to identify Th1 and Th2 cells as well as the T-regulatory subset in the endometrium of women with endometriosis associated with infertility upon transcription factors expression. Expression of T-bet, GATA3, and Foxp3 genes was examined using a method of polymerase chain reaction (PCR) in the eutopic endometrial samples of 20 women with endometriosis associated with infertility and 20 women with infertility of tubal origin...
2018: Central-European Journal of Immunology
Ismail Koyuncu, Abdurrahman Kocyigit, Ahmet Ozer, Sahabettin Selek, Adnan Kirmit, Hasan Karsen
Serologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector...
2018: Central-European Journal of Immunology
Rokhsareh Sadeghi, Mandana Sattari, Fatemeh Dehghan, Solmaz Akbari
Introduction: Interleukin-17 is a pro-inflammatory cytokine with a wide range of protective and destructive effects in periodontitis. The role of IL-23 is stabilisation and expansion of Th-17. The aim of this study was to assess whether patients with aggressive and chronic periodontitis exhibit different gingival crevicular fluid (GCF) concentrations of IL-17 and IL-23 compared with clinically healthy subjects. Material and methods: GCF samples were obtained from 32 patients: 10 with chronic periodontitis (CP), 12 with aggressive periodontitis (AgP), and 10 healthy controls (HC)...
2018: Central-European Journal of Immunology
Ewelina Witkowska-Sędek, Dominika Labochka, Anna Majcher, Beata Pyrżak
The aim of the study: was to investigate the coincidence of growth hormone deficiency (GHD) and celiac disease (CD) or inflammatory bowel disease (IBD) in patients referred for short stature, and to evaluate the baseline anthropometric parameters and the effectiveness of recombinant human growth hormone (rhGH) therapy in the first year in those patients (GHD+CD/IBD subgroup) in comparison to patients with GHD without CD or IBD (GHD-CD/IBD subgroup). Material and methods: The study was retrospective and included 2196 short patients (height SDS [Standard Deviation Score] ≤ -1...
2018: Central-European Journal of Immunology
Wahid Ali Khan
Aim of the study: Interferon alpha-induced arthritis and activation of the type 1 interferon pathway during rheumatoid arthritis (RA) has been well documented but the underlying mechanism remains unclear. This study addressed the binding specificity of antibodies with recombinant interferon alpha 2b (rIFN α -2b) in sera from different RA patients. Utilization of anti-hrIFN α -2b antibodies as a probe for estimation of interferon α -2b concentration in RA patients' synovial fluid (SF) was also investigated...
2018: Central-European Journal of Immunology
Aslihan Gulel, Huseyin Serhat Inaloz, Ayse Feyda Nursal, Tugce Sever, Sacide Pehlivan
Aim of the study: The aim of this study was to investigate the role TNF- α , IL-2, and IL-2RB variants in psoriasis (Ps) and to evaluate the association between these variants and clinical features. Material and methods: A total of 74 psoriatic patients and 74 healthy individuals were genotyped for these variants by PCR and/or RFLP. Results: The AA genotype of TNF- α (-308) was significantly more common in the patients (p = 0.013). TNF- α (-238) AA genotype was significantly increased in the patients (p = 0...
2018: Central-European Journal of Immunology
Natalia V Yunusova, Marina N Stakheyeva, Sergey V Molchanov, Sergey G Afanas'ev, Anastasia A Tsydenova, Larisa A Kolomiets, Nadejda V Cherdyntseva
Aim of the study: To compare the functional activity of natural killer cells depending on the presence of a malignant process and its dissemination. Material and methods: The study included 20 patients with Stage IIIB, C (FIGO, 2009) ovarian cancer, 10 patients with benign ovarian tumours (BOT), and 20 patients with colorectal cancer (T2-4N0-2M0). The control group consisted of 9 healthy donors. To evaluate the number and functional activity of NK cells, multicolour flow cytometry was performed...
2018: Central-European Journal of Immunology
Katarzyna Walkiewicz, Ewa Nowakowska-Zajdel, Paweł Kozieł, Małgorzata Muc-Wierzgoń
Colorectal cancer (CRC) is the third most common malignant neoplasm worldwide. In Poland, colorectal cancer ranks second in tumor incidence regardless of sex; moreover, there has been a steady increase in the incidence of CRC. CRC results from complex interactions between inherited susceptibility, clinical conditions and environmental/lifestyle-related risk factors such as physical inactivity, smoking, alcohol consumption, high-fat/low-fiber diet, and obesity/overweight. The activation of pathways associated with insulin resistance and insulin-like growth factors (IGF) appears to be the epidemiological link between the metabolic syndrome and the development of CRC, which is of particular importance...
2018: Central-European Journal of Immunology
Hamid Reza Mozaffari, Roohollah Sharifi, Masoud Sadeghi
Interleukin-6 (IL-6) is a cytokine that contributes to the pathogenesis of oral lichen planus (OLP). The aim of this meta-analysis study is the evaluation of IL-6 levels in the serum and saliva of patients with OLP compared with healthy controls. We searched the studies in 5 databases: PubMed/Medline, Scopus, ScienceDirect, Web of Science, and Cochrane Library, from 1983 to Oct 31, 2016. Eleven studies were analysed for the meta-analysis study. The reviewers independently evaluated the quality of each included study using the Newcastle-Ottawa Quality Assessment Scale (NOS)...
2018: Central-European Journal of Immunology
Małgorzata Litwiniuk, Małgorzata Radowicka, Alicja Krejner, Anna Śladowska, Tomasz Grzela
Aim of the study: The amniotic membrane is used as a dressing material, e.g. in ocular surgery or treatment of non-healing wounds. Of note, results of previous studies differ significantly, presumably due to the biological properties of amnion. Some authors suggest that these properties may depend on inter-donor variations, as well as the method of delivery. The aim of our study was to analyse the content of selected factors important for tissue regeneration in various areas of amnion samples originating from elective caesarean sections and on-term natural deliveries...
2018: Central-European Journal of Immunology
Katarzyna Gołąbek-Dropiewska, Justyna Pawłowska, Jacek Witkowski, Jerzy Lasek, Wojciech Marks, Mariusz Stasiak, Dawid Jaskólski, Aleksandra Kawecka, Piotr Łuczkiewicz, Bogusław Baczkowski
Introduction: Severe trauma causes damage to the protective barriers of the organism, and thus activates immunological reaction. Among substances secreted during this process pro-inflammatory cytokines are of high importance. The aim of the study: Severe trauma causing multiple injuries is more likely to lead to particularly intensive inflammatory reaction, which can sometimes lead to serious complications, even life-threatening. The aim of the study is to determine those parameters which may serve as predictors of infectious complications and to enable estimation of the patient's immunological status before the decision to introduce elective procedures...
2018: Central-European Journal of Immunology
Aneta M Binkowska, Grzegorz Michalak, Sławomir Pilip, Maria Kopacz, Robert Słotwiński
This study was aimed to give a better understanding of the mechanisms of early immune response to trauma by assessing the concentration of cytokines in peripheral blood. The study group comprised 32 patients admitted to the Emergency Department due to injury. Depending on the magnitude of the Injury Severity Score (ISS) trauma patients were divided into two groups. In group A (ISS ≥ 20), 13 patients had complications, and five died, while in group B (ISS < 20) only three patients had complications (e.g...
2018: Central-European Journal of Immunology
Akram Khan, Isfahan Tauseef, Bibi Aalia, Muhammad Azam Khan, Sadia Akbar, Nighat Sultana, Kashif S Haleem
The human serum is a vital component of the innate immunity of the host that acts as the first line of defence against invading pathogens. A key player in serum-mediated innate immune defence is a system of more than 35 proteins, collectively named as the complement system. After exposure of the pathogen, these proteins are activated in a cascade manner, ultimately forming a membrane attack complex (MAC) on the surface of the pathogen that directly lyses the bacterial cell. Formation of the MAC can be demonstrated in vitro by using serum bactericidal assay (SBA) that works in the absence of cellular components of blood after incubating the serum along with bacteria...
2018: Central-European Journal of Immunology
Sanaz Afraei, Reza Sedaghat, Farzaneh Tofighi Zavareh, Zahra Aghazadeh, Parvin Ekhtiari, Gholamreza Azizi, Abbas Mirshafiey
Introduction: Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS). EAE is mainly mediated by adaptive and innate immune responses that lead to an inflammatory demyelination and axonal damage. The aim of the present research was to examine the therapeutic efficacy of Peg interferon alpha 2a (Peg-IFN α-2a) as a serine protease inhibitor on EAE model. Material and methods: EAE induction was performed in female C57BL/6 mice by myelin oligodendrocyte glycoprotein (35-55) (MOG35-55 ) in Complete Freund's Adjuvant (CFA) emulsion, and Peg-IFN α-2a was used for the treatment of EAE...
2018: Central-European Journal of Immunology
Zhifang Ma, Liang Yue, Zhaoliang Xu, Sheng Zeng, Yukun Ma, Zhuoping Li, Wei Li, Dongwen Wang
Aim of the study: To investigate the effects of mast cells on the proliferation, invasion, and metastasis of prostate cancer cells. Material and methods: The mast cell P815 and prostate cancer LNCaP cells were chosen using a Transwell chamber to construct a two-cell cocultured in vitro model to observe the migration of mast cells to prostate cancer cells. Results: In the migration experiment, the migration rate of mast cells from the experimental group (%) was 10...
2018: Central-European Journal of Immunology
Saeed Bazregari, Gholamreza Azizi, Marzieh Tavakol, Mohammad Hosein Asgardoon, Fatemeh Kiaee, Naeimeh Tavakolinia, Amir Valizadeh, Hassan Abolhassani, Asghar Aghamohammadi
Introduction: Primary immunodeficiency diseases (PIDs) are a heterogeneous group of genetic immune disorders. PID patients suffer from a variety of complications. The aim of this study was to determine the infectious and non-infectious complications among PID patients. Material and methods: This retrospective cohort study was performed on recorded data of 202 PID patients who were diagnosed with eight major categories: common variable immunodeficiency (CVID), X-linked agammaglobulinemia, hyper-IgM syndrome, hyper IgE syndrome, chronic granulomatous disease (CGD), ataxia telangiectasia, hereditary angioedema and leukocyte adhesion deficiency...
2017: Central-European Journal of Immunology
Oksana Boyarchuk, Liubov Volyanska, Liubov Dmytrash
22q11.2 deletion syndrome (22q11.2 DS) is a disorder that has multiple symptoms and affects various organs and systems. Despite the great variability of clinical manifestations, common 22q11.2 DS includes congenital heart defect, immunodeficiency, characteristic facial features, palatal defects, developmental and/or learning disabilities, and hypocalcaemia. We present the cases of three patients with 22q11.2 DS that we have observed. Heart defects were revealed in all cases, and tetralogy of Fallot in one of them...
2017: Central-European Journal of Immunology
Hulya Anil, Koray Harmanci, Rahmi T Tekin, Abdulkadir Kocak
Drug rash with eosinophilia and systemic symptoms (DRESS) syndrome is a rare, potentially life-threatening, drug-induced hypersensitivity reaction that includes rash, hematologic abnormalities, lymphadenopathy, and internal organ involvement. The pathogenesis of DRESS syndrome is partially understood. Various medications have been described as the cause of DRESS syndrome. Phenytoin and allopurinol are the most commonly reported culprit drugs, although more than 50 drugs can induce DRESS syndrome. Members of the cytochrome P450 (CYP) superfamily are the most commonly involved enzymes in metabolism of drugs such as phenytoin...
2017: Central-European Journal of Immunology
Hanna Szymanik-Grzelak, Joanna Przychodzeń, Anna Stelmaszczyk-Emmel, MaŁgorzata Pańczyk-Tomaszewska
Systemic lupus erythematosus (SLE) concomitant with thrombotic thrombocytopenic purpura (TTP) in children is rarely diagnosed. We report a case of a 15-year-old girl with butterfly patch, generalized edema, leg pain, anemia (Hb 74 g/l), thrombocytopenia (5 x 109 /l), schistocytes in peripheral blood smear, acute kidney injury (eGFR 27 ml/min/1.73 m2 ), proteinuria, and erythrocyturia. The direct Coombs test was positive, and warm antibodies (IgG) were detected on red blood cells. ANA in titer 1 : 2560, low serum C3 and C4 complement level, ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) activity < 4% and the presence of ADAMTS13 inhibitor were detected...
2017: Central-European Journal of Immunology
Maria Daniel, Anna Adamowicz-Salach, Hanna Szymanik-Grzelak
Paroxysmal cold haemoglobinuria (PCH) is a form of autoimmune haemolytic anaemia (AIHA) characterised by a sudden onset of haemoglobinuria, either spontaneously or following exposure to cold. In children, it is commonly seen following a viral illness or after immunisation. Diagnosis of PCH is confirmed by a positive Donath Landsteiner (DL) test in which biphasic haemolysins are detected. However, in a real clinical setting, the serological diagnosis of PCH is not always easy. PCH can cause tubular renal injury, which in turn can lead to renal impairment...
2017: Central-European Journal of Immunology
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