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Zhen Wang, Yao Xu, Menglong Wang, Jing Ye, Jianfang Liu, Huimin Jiang, Di Ye, Jun Wan
No abstract text is available yet for this article.
November 9, 2018: EBioMedicine
Dan Guo, Hua Jiang, Yiqiu Chen, Jing Yang, Ziqiang Fu, Jing Li, Xiumei Han, Xian Wu, Yankai Xia, Xinru Wang, Liping Chen, Qiuqin Tang, Wei Wu
BACKGROUND: Several studies have reported microRNAs (miRNAs) could regulate the placental development, though the role and mechanism of miRNAs in the development of non-diabetic macrosomia (NDFMS) remains unclear. METHODS: To identify the aberrantly expressed key miRNAs in placenta of NDFMS, we employed a strategy consisting of initial screening with miRNA microarray and further validation with quantitative RT-PCR assay (qRT-PCR). In vitro cellular model and a mouse pregnancy model were used to delineate the functional effects of key miRNA on proliferation, invasion, and migration...
November 9, 2018: EBioMedicine
Jing-Lu Jin, Di Sun, Ye-Xuan Cao, Hui-Wen Zhang, Yuan-Lin Guo, Na-Qiong Wu, Cheng-Gang Zhu, Ying Gao, Qiu-Ting Dong, Geng Liu, Qian Dong, Jian-Jun Li
BACKGROUND: Severe hypertriglyceridemia (SHTG, TG ≥5·65 mmol/L), a disease, usually resulting from a combination of genetic and environmental factors, may increase the risk of acute pancreatitis (AP). However, previous genetic analysis has been limited by lacking of related observation of gene to AP. METHODS: The expanding genetic sequencing including 15 TG-related genes (LPL, LMF1, APOC2, GPIHBP1, GCKR, ANGPTL3, APOB, APOA1-A4-C3-A5, TRIB1, CETP, APOE, and LIPI) was performed within 103 patients who were diagnosed with primary SHTG and 46 age- and sex-matched normal controls...
November 9, 2018: EBioMedicine
Zhongshi Lyu, Zhaomin Mao, Qianyin Li, Yan Xia, Yamin Liu, Qingling He, Yingchun Wang, Hui Zhao, Zhimin Lu, Qin Zhou
BACKGROUND: The renal tubules, which have distant metabolic features and functions in different segments, reabsorb >99% of approximately 180 l of water and 25,000 mmol of Na +  daily. Defective metabolism in renal tubules is involved in the pathobiology of kidney diseases. However, the mechanisms underlying the metabolic regulation in renal tubules remain to be defined. METHODS: We quantitatively compared the proteomes of the isolated proximal tubules (PT) and distal tubules (DT) from C57BL/6 mouse using tandem mass tag (TMT) labeling-based quantitative mass spectrometry...
November 9, 2018: EBioMedicine
Jiang-Bo Zhang, Shao-Yi Huang, Tong-Min Wang, Si-Qi Dong, Yong-Qiao He, Xiao-Hui Zheng, Xi-Zhao Li, Fang Wang, Mu Jianbing, Wei-Hua Jia
BACKGROUND: Epstein-Barr virus (EBV) infection is a crucial risk factor for nasopharyngeal carcinoma (NPC), but the mechanism for its elevated activation level in NPC endemic areas remains unclear. This study aims to identify the EBV natural variations contributed to the different reactivation potential between NPC endemic and non-endemic areas. METHODS: 1030 subjects were recruited in China, including 303 healthy individuals from two NPC non-endemic areas, 483 healthy people from three endemic areas and 244 NPC patients...
November 9, 2018: EBioMedicine
María José Gosalbes, Joan Compte, Silvia Moriano-Gutierrez, Yvonne Vallès, Nuria Jiménez-Hernández, Xavier Pons, Alejandro Artacho, M Pilar Francino
BACKGROUND: The relationship between the gut microbiome and the human host is dynamic and we may expect adjustments in microbiome function if host physiology changes. Metatranscriptomic approaches should be key in unraveling how such adjustments occur. METHODS: We employ metatranscriptomic sequencing analyses to study gene expression in the gut microbiota of infants through their first year of life, and of their mothers days before delivery and one year afterwards...
November 8, 2018: EBioMedicine
Samane Abbasy, Fazlollah Shahraki, Arvin Haghighatfard, Masoume Ghasemzadeh Qazvini, Sahel Towfigh Rafiei, Elnaz Noshadirad, Mahdi Farhadi, Horie Rezvani Asl, Atena Alsadat Shiryazdi, Rana Ghamari, Zeinab Tabrizi, Rashed Mehrfard, Fereshteh Esmaili Kakroudi, Mahsima Azarnoosh, Faeghe Younesi, Narges Parsamehr, Nooriyeh Garaei, Soroush Abyari, Maede Salehi, Maryam Gholami, Pardis Zolfaghari, Seyede Mahsa Bagheri, Melika Pourmehrabi, Elham Rastegarimogaddam, Elnaz Nobakht, Elmira Nobakht, Rayan Partovi
BACKGROUND: Autism spectrum disorder (ASD) is a pediatric heterogeneous psychiatric and neurodevelopmental disorder with social and communication deficits, language impairment and ritualistic or repetitive behaviors. ASD has significant genetic bases but candidate genes and molecular mechanisms of disorder are not clarified. Neuregulin1 (NRG1) gene, located in 8p12 is involved in development of central nervous system and was indicated as candidate gene in schizophrenia. METHODS: mRNA level of types I, II and III of NRG1 gene were studied in peripheral blood of 1540 ASD patients (IQ > 70) and 1490 control children by quantitative Real Time PCR...
November 8, 2018: EBioMedicine
Rana Raoof, Sebastian Bauer, Hany El Naggar, Niamh M C Connolly, Gary P Brennan, Elizabeth Brindley, Thomas Hill, Hazel McArdle, Elaine Spain, Robert J Forster, Jochen H M Prehn, Hajo Hamer, Norman Delanty, Felix Rosenow, Catherine Mooney, David C Henshall
BACKGROUND: There are no blood-based molecular biomarkers of temporal lobe epilepsy (TLE) to support clinical diagnosis. MicroRNAs are short noncoding RNAs with strong biomarker potential due to their cell-specific expression, mechanistic links to brain excitability, and stable detection in biofluids. Altered levels of circulating microRNAs have been reported in human epilepsy, but most studies collected samples from one clinical site, used a single profiling platform or conducted minimal validation...
November 2, 2018: EBioMedicine
Yuan Qin, Hui-Juan Liu, Meng Li, Deng-Hui Zhai, Yuan-Hao Tang, Lan Yang, Kai-Liang Qiao, Jia-Huan Yang, Wei-Long Zhong, Qiang Zhang, Yan-Rong Liu, Guang Yang, Tao Sun, Cheng Yang
BACKGROUND: Hypoxia commonly occurs in solid tumors. The hypoxia in the center of solid tumors considerably decreases the chemosensitivity of tumor cells and induces epithelial-mesenchymal transition (EMT) as well as drug resistance of antitumor drugs. METHODS: Here, the effects of salidroside (Sal) combined with platinum drugs on human hepatocellular carcinoma were examined in vitro and in vivo. We investigated the antitumor effects of Sal by inhibiting the drug resistance and explained its mechanism in inhibiting tumor growth...
November 2, 2018: EBioMedicine
Martijn M T Vink, Sami M Nahzat, Habiburrahman Rahimi, Cyril Buhler, Bashir A Ahmadi, Mohammad Nader, Fazal R Zazai, Abdul S Yousufzai, Merlin van Loenen, Henk D F H Schallig, Albert Picado, Israel Cruz
BACKGROUND: Kabul (Afghanistan) is a major focus of cutaneous leishmaniasis (CL) caused by Leishmania tropica. Microscopy remains the reference test for diagnosis despite its low performance. We evaluated whether Loopamp™ Leishmania Detection Kit (Loopamp) and CL Detect™ Rapid Test (CL Detect), detecting Leishmania DNA and antigen, respectively could improve CL diagnosis. METHODS: A diagnostic accuracy study with prospective inclusion was conducted in a leishmaniasis reference clinic in Kabul...
November 2, 2018: EBioMedicine
Zi-Ye Li, Lin Yang, Xiao-Jun Liu, Xing-Zhe Wang, Yu-Xia Pan, Jian-Min Luo
No abstract text is available yet for this article.
November 2, 2018: EBioMedicine
Zhiyong Liu, Wei Li, Yanan Pang, Zaixin Zhou, Shupeng Liu, Kai Cheng, Qin Qin, Yin Jia, Shanrong Liu
BACKGROUND: Splicing factor 3b subunit 4 (SF3B4) is a splicing factor and potential oncogene in hepatocellular carcinoma (HCC); however, its regulatory mechanism is yet unclear. We aimed to determine the role of SF3B4 in HCC and the underlying mechanism. METHODS: To investigate the association between alternative splicing events and miRNAs, putative miRNAs were screened using TargetScan. Expression levels of and prognostic information for SF3B4 and miRNAs were determined based on public genomic data and clinical samples...
October 31, 2018: EBioMedicine
Shinya Aoyama, Shuichi Kojima, Keisuke Sasaki, Ryosuke Ishikawa, Mizuho Tanaka, Takeru Shimoda, Yuta Hattori, Natsumi Aoki, Kengo Takahashi, Rina Hirooka, Miku Takizawa, Atsushi Haraguchi, Shigenobu Shibata
BACKGROUND: Atrogin1, which is one of the key genes for the promotion of muscle atrophy, exhibits day-night variation. However, its mechanism and the role of its day-night variation are largely unknown in a muscle atrophic context. METHODS: The mice were induced a muscle atrophy by hindlimb-unloading (HU). To examine a role of circadian clock, Wild-type (WT) and Clock mutant mice were used. To test the effects of a neuronal effects, an unilateral ablation of sciatic nerve was performed in HU mice...
October 31, 2018: EBioMedicine
Cristina Cunha, Agostinho Carvalho
No abstract text is available yet for this article.
October 31, 2018: EBioMedicine
Alexander Pott, Steffen Just
No abstract text is available yet for this article.
October 31, 2018: EBioMedicine
Peng Wu, Shuo Yang, Sandeep Singh, Fujun Qin, Shailesh Kumar, Ling Wang, Ding Ma, Hui Li
BACKGROUND: Gene fusions and fusion products have been proven to be ideal biomarkers and drug targets for cancer. Even though a comprehensive study of cervical cancer has been conducted as part of the Cancer Genome Atlas (TCGA) project, few recurrent gene fusions have been found, and none above 3% of frequency. METHODS: We believe that chimeric fusion RNAs generated by intergenic splicing represent a new repertoire of biomarkers and/or therapeutic targets. However, they would be missed when only genome sequences and fusions at DNA level are considered...
October 31, 2018: EBioMedicine
Daniel L McCartney, Anna J Stevenson, Robert F Hillary, Rosie M Walker, Mairead L Bermingham, Stewart W Morris, Toni-Kim Clarke, Archie Campbell, Alison D Murray, Heather C Whalley, David J Porteous, Peter M Visscher, Andrew M McIntosh, Kathryn L Evans, Ian J Deary, Riccardo E Marioni
BACKGROUND: Multiple studies have made robust associations between differential DNA methylation and exposure to cigarette smoke. But whether a DNA methylation phenotype is established immediately upon exposure, or only after prolonged exposure is less well-established. Here, we assess DNA methylation patterns from peripheral blood samples in current smokers in response to dose and duration of exposure, along with the effects of smoking cessation on DNA methylation in former smokers. METHODS: Dimensionality reduction was applied to DNA methylation data at 90 previously identified smoking-associated CpG sites for over 4900 individuals in the Generation Scotland cohort...
October 30, 2018: EBioMedicine
Takeru Tsujimoto, Hideki Sudo, Masahiro Todoh, Katsuhisa Yamada, Koji Iwasaki, Takashi Ohnishi, Naoki Hirohama, Takayuki Nonoyama, Daisuke Ukeba, Katsuro Ura, Yoichi M Ito, Norimasa Iwasaki
BACKGROUND: The current surgical procedure of choice for lumbar intervertebral disc (IVD) herniation is discectomy. However, defects within IVD produced upon discectomy may impair tissue healing and predispose patients to subsequent IVD degeneration. This study aimed to investigate whether the use of an acellular bioresorbable ultra-purified alginate (UPAL) gel implantation system is safe and effective as a reparative therapeutic strategy after lumbar discectomy. METHODS: Human IVD cells were cultured in a three-dimensional system in UPAL gel...
October 30, 2018: EBioMedicine
Francis Gabriel Moussy, Adriana Velazquez Berumen, Madhukar Pai
No abstract text is available yet for this article.
October 30, 2018: EBioMedicine
Dan Jin, Jiwei Guo, Deqiang Wang, Yan Wu, Xiaohong Wang, Yong Gao, Cuijie Shao, Xin Xu, Shuying Tan
BACKGROUND: Activation of the oncogene YAP has been shown to be related to lung cancer progression and associates with poor prognosis and metastasis. Metformin is a drug commonly used in the treatment of diabetes and with anticancer activity. However, the mechanism through which metformin inhibits tumorigenesis via YAP is poorly understood. METHODS: The mRNA and protein expressions were analyzed by RT-PCR and western blot. The cellular proliferation was detected by CCK8 and MTT...
October 30, 2018: EBioMedicine
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