Mohammad Hamed Shahsavari, Gholamali Moghaddam, Kele Amaral Alves, Benner Geraldo Alves, Laritza Ferreira de Lima, Golshan Azimi, Deysi Juana Dipaz Berrocal, Luciana Mascena Silva, Yago Pinto da Silva, Diego Alberto Montano Vizcarra, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
Although cryopreservation of ovarian tissue has advanced greatly, it remains a challenge, and protocols should be optimized to handle the heterogeneous nature of ovarian samples. In an effort to address this factor, the present study evaluated the effects of corpus luteum (CL) and side of ovaries (right versus left) on cellular morphology and viability of vitrified bovine ovarian fragments in a closed system. The ovaries were categorized according to whether they had a CL and which side they were on, and then divided into six groups: 1) CL+ (with CL) group; 2) CL- (without CL) group; 3) right ovaries group; 4) left ovaries group; 5) fresh control group (ovaries without vitrification or culture that were not selected for CL or ovarian side) and 6) In vitro culture medium control group (non-vitrified ovaries that were not selected for the presence or absence of CL or side of the ovaries)...
2023: Veterinary Research Forum