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Veterinary Research Forum

Pouria Motamed Chaboki, Arash Ghalyanchilangeroudi, Vahid Karimi, Hamed Abdollahi, Hossein Maghsoudloo, Hossein Hosseini, Reza Khaltababdi Farahahni, Seyed Ali Ghafouri, Mohammad Hossein Falah, Hussein Rezaee, Masoumeh Jabbarifakhr, Fatemeh Sadat Mousavi
Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and swollen head syndrome in chickens. Four aMPV subgroups (A-D) have been reported previously based on their genetic and antigenic differences. Evidence suggests that the live bird markets (LBMs) play an important role in the epidemiology of the avian viral diseases. A total number of 450 oropharyngeal samples from eight different species of birds (migratory and local) were collected from LBMs of Gilan province, Iran, from October to December 2016...
2018: Veterinary Research Forum
Farkhondeh Pooyanfar, Tahereh Foroutan, Mojtaba Dashtizad
Although the rate of blastocysts implantation of embryos is higher than previous stages but their survival rate is lower than them, which could be attributed to the completely filled blastocoel cavity with liquid and increased possibility of the formation of ice crystals. This liquid could prevent the penetration of cryoprotecting materials into the embryos. In this study, we reduced the volume of blastocoel before vitrification and compared survival rate and quality of in vitro embryos through klf4 gene expression with control group...
2018: Veterinary Research Forum
Farnaz Malekifard, Minoo Ahmadpour
Giardia duodenalis is one of the most prevalent intestinal protozoa infecting humans and domestic animals. The aim of this study was to identify subspecies of G. duodenalis by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method from fecal samples of naturally infected cattle in the Urmia, West Azerbaijan province, Iran. Overall, 246 fecal specimens were collected from the cattle (diarrheic and healthy) and microscopically examined for G. duodenalis. The PCR-RFLP analysis of glutamate dehydrogenase (gdh) locus was used to identify the genotypes found in cattle...
2018: Veterinary Research Forum
Parviz Hassanzadeh, Mehran Moradi, Nasim Vaezi, Mir-Hassan Moosavy, Razzagh Mahmoudi
In recent years, use of edible coatings as carriers of food additives and antimicrobial compounds has been considered in fishery products. This study was carried out to evaluate the effects of 2.00% chitosan coating singly and combined with 0.10% grape seed extract (GSE) on microbial (mesophils and psychrophils counts), chemical (thiobarbituric acid; TBA), pH and peroxide value (PV) and sensorial properties of rainbow trout fillet stored at 4 ° C over a period of 15 days. The coating had a significant effect in reducing aerobic mesophilic and psychrophilic bacteria counts...
2018: Veterinary Research Forum
Masoud Reza Seyfi Abad Shapouri, Mohammad Rashno, Pezhman Mahmoodi, Mahshid Aria
Monoclonal antibodies (MAbs) are invaluable molecules which have several advantages over polyclonal immunoglobulins (Igs) including consistency and higher specificity and hence can be used in biological researches, diagnosis and treatment of diseases. The present study was conducted to produce monoclonal antibody against chicken IgG. The IgG molecules were purified from chicken serum and used as antigens to immunize several mice. Thereafter, a well-immunized mouse was chosen and used for fusion process. After production of hybridoma cells, several rounds of cloning were carried out and produced MAbs were examined by various immunological assays including enzyme-linked immunosorbent assay (ELISA) and western and dot blotting...
2018: Veterinary Research Forum
Neda Abedpour, Mojdeh Salehnia, Nassim Ghorbanmehr
Lysophosphatidic acid (LPA) known as a serum-derived growth factor, is involved in several cell physiological functions in the female reproductive system including: oocyte maturation, in vitro fertilization and embryo implantation by its transmembrane G protein-coupled receptors. The aim of the present study was to examine the effect of LPA on in vitro follicular development of mouse ovarian tissue. Neonatal mouse ovarian tissues were cultured in five different concentrations of LPA (0, 5, 10, 20 and 40 µM)...
2018: Veterinary Research Forum
Mostafa Soufizadeh, Rasoul Pirmohammadi, Yunes Alijoo, Hamed Khalilvandi Behroozyar
Indigestible neutral detergent fibers (iNDF) accurately predict forage digestibility when measured in situ . The objective of this study was to determine the effects of rumen incubation times on the estimated concentrations of iNDF for four forages (alfalfa hay, corn silage, wheat straw and orchard grass), four concentrates (barley grain, soybean meal, beet pulp and wheat bran) and two total mixed ration samples in dairy cows. The iNDF contents of the samples were evaluated in 10 feeds using three ruminally cannulated Holstein cows in a completely randomized design...
2018: Veterinary Research Forum
Mohammad Amin Gholami, Masihollah Forouzmand, Mokhtar Khajavi, Shima Hossienifar, Reza Naghiha
The purpose of this study was to investigate the effect of different corn processing methods on rumen microbial flora, histomorphometry and fermentation in fattening male lambs. Twenty male lambs (average age and weight of 90 days and 25.00 ± 1.10 kg, respectively) were used in a completely randomized design including four treatments and five replicates each over 80 days long period: 1) Lambs fed ground corn seeds; 2) Lambs fed steam-rolled corn; 3) Lambs fed soaked corn seeds (24 hr) and 4) Lambs fed soaked corn seeds (48 hr)...
2018: Veterinary Research Forum
Mohammad Afkhami-Ardakani, Shapour Hasanzadeh, Rasoul Shahrooz, Norouz Delirezh, Hassan Malekinejad
Cyclophosphamide (CP) is known to reduce fertility. The protective effects of Spirulina plantesis (SP) against CP-induced testicular toxicity were investigated. Male Wistar rats were categorized into eight groups (n = 7). Four groups of rats were administered CP at a dose of 5 mg in 5 mL distilled water kg-1 per day orally. Two of these groups were received SP (500 and 1000 mg kg-1 per day) orally after CP administration. One of these groups was also received vitamin E (100 mg kg-1 per day) intraperitoneally...
2018: Veterinary Research Forum
Akbar Anaeigoudari, Fatemeh Norouzi, Azam Abareshi, Farimah Beheshti, Azita Aaghaei, Mohammad Naser Shafei, Zahra Gholamnezhad, Mahmoud Hosseini
In the present study the protective effect of Nigella sativa ( N. sativa ) on synaptic plasticity impairment induced by lipopolysaccharide (LPS) in rats was investigated. Fifty-eight rats were grouped and treated as follows: 1) control (saline), 2) LPS, 3) LPS -N. sativa , and 4) N. sativa. In a Morris water maze test, the escape latency and traveled path to find the platform as well as time spent and the traveled distance in target quadrant (Q1 ) were measured. Long term potentiation (LTP) from CA1 area of hippocampus followed by high frequency stimulation to Schafer collateral was studied and slope, slope 10-90% and amplitude of field excitatory field potential (fEPSP) were calculated...
2018: Veterinary Research Forum
Vahid Fathipour, Zohreh Khaki, Seyyed Mehdi Nassiri
Recently, matrix metalloproteinases (MMP), in particular the gelatinases MMP-2 and MMP-9, generally considered as tumor markers for clinical applications. A longitudinal 2-years follow-up survey was performed on dogs with cutaneous tumor. Serum samples were obtained from 22 dogs with different cutaneous tumors and 22 health dogs at the time of surgery and one month, three months and one year after surgery. Gelatin zymography, hematological and biochemical assessment were performed for all serum samples. The serum alkaline phosphatase activity in dogs with malignant tumors was significantly higher than that in dogs with benign tumors and control cases...
2018: Veterinary Research Forum
Somayeh Sadeghi, Leili Aghebati-Maleki, Samira Nozari, Jafar Majidi
Antibodies are a class of biomolecules that has an important role in the immune system and lots of applications in biotechnological methods and in pharmaceutics. Production and purification of antibodies in laboratory animals is one of the first ways to manufacture of these prominent tools. The obtained antibodies from these process could be used in various types of bioassay techniques such as enzyme linked immunosorbent assay (ELISA), radioimmunoassay, etc. Also, antibodies employed in diagnostics applications in humans and other animals in order to detect specific antigens...
2018: Veterinary Research Forum
Seyedeh Missagh Jalali, Masood Ghorbanpour, Mohammad Razi Jalali, Aria Rasooli, Pegah Safaie, Farideh Norvej, Imaneh Delavari
The main objectives of this study were to determine the occurrence and potential causative factors of Immune-mediated hemolytic anemia (IMHA) in native cattle and water buffaloes from southwest of Iran. Fifty-three anemic animals (37 cattle and 16 buffaloes) were studied. A full clinical history and physical examinations were undertaken for all animals. Four clinically healthy cattle and four healthy buffaloes were also used as control animals. Blood samples were subjected to a complete blood count, Coombs' test, erythrocyte osmotic fragility test and serum biochemical analysis...
2018: Veterinary Research Forum
Alireza Talebi, Manoochehr Alimehr, Mohammad Hossein Alavi, Gholamreza Najafi, Naeimeh Simaei
Fertility reduction due to sub-fertile males is a major concern in breeder flocks. Phenotypic traits of broiler breeder males and their relationships with fertility can be used as reliable indicators for identification and removal of sub-fertile males from the breeder flocks. This study was conducted to investigate semen traits (semen volume, sperm motility, sperm viability and sperm count) and testes histomorphometric features including tubule differentiation index (TDI), spermiation index (SPI), Sertoli cell index (SCI) and mitotic index (MI) of broiler breeder males with the same age but different phenotypic traits...
2018: Veterinary Research Forum
Mohammad Farajli Abbasi, Bahador Shojaei, Omid Azari
Congenital agenesis of lumbar vertebrae was diagnosed in a day-old female lamb based on radiology and clinical examinations. There was no neurological deficit in hindlimb and forelimb associated with standing disability. Radiography of the abdominal region revealed absence of lumbar vertebrae. Necropsy confirmed clinical and radiographic results. No other anomaly or agenesis was seen macroscopically in the abdominal and thoracic regions as well as vertebral column. Partial absence of vertebral column has been reported in human and different animal species, as an independent occurrence or associated with other organs anomalies...
2017: Veterinary Research Forum
Parvin Abedi, Saleh Tabatabaei Vakili, Morteza Mamouei, Ali Aghaei
The aim of this study was to evaluate the effects of dietary vitamin E on reproductive and productive parameters in Japanese quails. A total number of 240 female and 80 male Japanese quail were divided into five treatments with four replications in a completely randomized design. Experimental diets were zero control, 30, 60, 120 and 240 mg kg-1 of vitamin E. Fertility and total hatchability were not affected by treatments. But, the lowest hatch of fertile eggs and the highest embryonic death were observed in control group ( p < 0...
2017: Veterinary Research Forum
Noushin Derakhshandeh, Hassan Sharifiyazdi, Mohammad Abbaszadeh Hasiri
Ehrlichiosis is a zoonotic disease which has been reported from some regions of Iran. This study was aimed to determine the presence and prevalence of ehrlichiosis in suspected dogs referred to the Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran using polymerase chain reaction (PCR). Blood samples were collected from 98 suspected dogs with at least one of the five following findings: thrombocytopenia, anemia (hematocrit < 37.00%), gastrointestinal signs and respiratory and/or central nervous system diseases...
2017: Veterinary Research Forum
Alireza Nourian, Ali Soleimanzadeh, Ali Shalizar Jalali, Gholamreza Najafi
Bisphenol-S (BPS) is a new bisphenol-A substitute widely used in many plastic products. Bisphenol-A as a main member of bisphenol family has been known as an endocrine system disrupter chemical compound. Like other members of bisphenol family, there is public health concern about the toxic effects of BPS on reproductive system, thus, we examined BPS effects on in vitro fertilization (IVF) potential and oxidative stress status in a murine model. Adult female mice (n = 70) were randomly divided into control and BPS-treated groups...
2017: Veterinary Research Forum
Somayeh Namroodi, Annalisa Zaccaroni, Hassan Rezaei, Seyyedeh Malihe Hosseini
Anthropogenic environmental changes are hypothesized as main reasons for animal species population declines. Heavy metals contamination is one of the worst threats to animals among human-caused threats. As most of the heavy metals bioaccumulate in organisms, analyzing concentrations of heavy metals in long living animals, such as turtles, would be very useful for biomonitoring of environmental quality. The European pond turtle is classified as a Near Threatened in the red list of International Union for Conservation of Nature...
2017: Veterinary Research Forum
Abdolhossein Jangaran Nejad, Rahim Peyghan, Hossein Najafzadeh Varzi, Ali Shahriyari
The aim of this study was to evaluate pharmacokinetic profiles of florfenicol after a single dose of intravenous (5.00 mg kg-1 body weight) and oral (40.00 mg kg-1 body weight) administrations in common carp ( Cyprinus carpio ). The residue depletion of florfenicol was also investigated after oral administration (10.00 mg kg-1 body weight) and bath treatment (5.00 mg L-1 ) for 10 consecutive days. Pharmacokinetics of florfenicol in plasma after a single dose administration, at 10 time points (0.50, 1, 2, 4, 8, 12, 24, 72, 120 and 168 hr) and florfenicol concentrations in tissues (plasma, liver and muscle) at three time points (1, 7 and 14 days) after 10 consecutive days, were analyzed by high performance liquid chromatography...
2017: Veterinary Research Forum
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