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Veterinary Research Forum

Zahra Nikousefat, Mohammad Hashemnia, Moosa Javdani, Ali Ghashghaii
Feline lower urinary tract diseases are known to be life threatening conditions in cats, especially when they occur as obstructive diseases in males. Early diagnosis and treatment is necessary, otherwise it may lead to death. A 3-year-old male Persian cat was referred to the clinic with a history of anuria, lethargy, loss of appetite and exploratory cystotomy 6 months ago due to urethral obstruction following urolithiasis. Urinary bladder was enlarged and painful on palpation and urine accumulation was observed in ultrasonography...
2018: Veterinary Research Forum
Hiwa Baraz, Hossein Jahani-Azizabadi, Osman Azizi
Two trials were conducted to investigate the effects of disodium fumarate (DSF; 0.00, 8.00, 10.00 and 12.00 mM) and thyme essential oil (TEO; 0.00, 100.00, 200.00, 300.00 and 400.00 µL L-1 ) solely and simultaneously (10.00 mM DSF along with 100.00, 200.00, 300.00 and 400 µL L-1 TEO) on in vitro ruminal fermentation of a 50:50 alfalfa hay to concentrate diet. The DSF and TEO did not affect crude protein disappearance, gas production, microbial crude protein synthesis and hydrogen recovery. The DSF addition linearly increased partitioning factor (PF) and molar proportion of propionate and decreased acetate: propionate ratio and methane production...
2018: Veterinary Research Forum
Gholam Hossein Farjah, Masoumeh Mohammad Pour, Mohammad Hassan Khadem-Ansari, Mojtaba Karimipour, Bagher Pourheidar
Operation on the thoraco-abdominal aorta may lead to paraplegia or paraparesis is after spinal ischemia/reperfusion (I/R) injury. In this study, we investigated the protective effect of the spinach extract on spinal cord I/R injury. Thirty-five male Sprague-Dawley rats were divided into five groups: Intact, sham surgery, normal saline (NS), low dose spinach extract (20 mg kg-1 ), high dose spinach extract (50 mg kg-1 ). Neurological function, biochemical and histological evaluations were performed in 72 hr after ischemia...
2018: Veterinary Research Forum
Mohammadreza Taghdiri, Guiti Karim, Shahabeddin Safi, Abbas Rahimi Foroushani, Abbasali Motalebi
No abstract text is available yet for this article.
2018: Veterinary Research Forum
Soghra Abbaszadeh, Farhad Farrokhi-Ardabili, Hassan Malekinejad, Iraj Bernousi
This study was aimed to investigate the effects of 17
2018: Veterinary Research Forum
Kareem Morsy, Mohammed Shazly, Mahrashan Abdel-Gawad, Nahed Saed
Monogenea is one of the most species-rich groups of parasitic flatworms worldwide with many species described from African freshwater fish. Little is known about the diversity and geographic distribution of monogenean parasites infesting the Red Sea fishes in Egypt. In the present study, a total of 45 specimens of the brushtooth lizardfish Saurida undosquamis (family: Synodontidae) and 35 specimens of the red porgy seabream Pagrus pagrus (family: Sparidae) was examined for monogenean infestation. Samples were collected from water locations at Hurghada coasts along the Red Sea in Egypt...
2018: Veterinary Research Forum
Ali Mojaddar Langroodi, Hossein Tajik, Tooraj Mehdizadeh
Beef is susceptible to rapid spoilage due to its high amount of protein (18.00%) and moisture (72.00%). Food industries have recently found methods to extend beef shelf-life. The influence of beef dipping in hydro-alcoholic extract of sumac (SE) and chitosan (CH) coating incorporated with Zataria multiflora essential oil (ZEO) on microbial, chemical and sensory quality of beef was evaluated during refrigerated storage. Total viable counts (TVC), lactic acid bacteria, Pseudomonas spp., Enterobacteriaceae and yeasts-molds, total volatile nitrogen (TVN), thiobarbituric acid reactive substance values (TBARS) and peroxide value (PV) were founded to be significantly lower in all treatment groups compare to control groups during storage time...
2018: Veterinary Research Forum
Mahboobeh Amoushahi, Mojdeh Salehnia
The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A ( TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were considered as control. All ovaries were cultured for seven days, and their isolated preantral follicles were cultured in three-dimensional culture system...
2018: Veterinary Research Forum
Farzaneh Azizi, Mohammad Nasrollahzadeh Masouleh, Seeyamak Mashhadi Rafie, Ahmad Asghari, Saied Bokaie
The aim of this study was to determine the effects of intramuscular injection of medetomidine on intra-renal arteries resistive and pulsatility indices by duplex Doppler ultrasonography in clinically normal adult domestic shorthair cats. For this purpose, twenty-six neutered adult healthy domestic shorthair cats (13 females and 13 males) were evaluated. B-mode, color Doppler and pulsed wave Doppler ultrasonography of right and left kidneys were performed to record the resistive index (RI) and pulsatility index (PI) of intra-renal arteries...
2018: Veterinary Research Forum
Mahsa Hasanzadeh-Moghadam, Mohammad Hassan Khadem-Ansari, Gholam Hossein Farjah, Yousef Rasmi
Myocardial infarction is commonly considered as a leading cause of cardiovascular disease taking the lives of seven million people annually. Liver dysfunction is associated with cardiac diseases. The profile of abnormal liver functions in heart failure is not clearly defined. This study was designed to investigate the protective effects of betaine on liver injury after myocardial infarction induced by isoprenaline in rats. Forty-eight male rats were divided into four groups: the control group received normal diet and the experimental groups received 50, 150, and 250 mg kg-1 body weight of betaine daily through gastric gavages for 60 days...
2018: Veterinary Research Forum
Mahmood Khaksary-Mahabady, Kaveh Khazaeel, Mahdi Pourmahdi Borujeni, Behnam Yazdanjoo
Immune system plays crucial role in body and lymph nodes are essential parts of this system for combating pathogens. However, no study has ever been conducted on morphometric development of sheep lymph nodes in fetal period. Thus, this study attempted to examine the morphometric characteristics of a number of important lymph nodes of some lymphocenters of sheep during fetal period. To this end, 60 pieces of sheep fetuses collected from Ahvaz slaughterhouse were fixated in 10% formalin and then divided into four categories based on crown-rump length (CRL) following gender and weight determinations...
2018: Veterinary Research Forum
Amin Gholamhosseini, Vahideh Taghadosi, Nima Shiry, Mostafa Akhlaghi, Hassan Sharifiyazdi, Siyavash Soltanian, Nasrollah Ahmadi
The purpose of the present study was to isolate and identify the pathogenic agents in Acipenser stellatus (Pallas, 1771) and Huso huso, (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemia symptoms such as decreasing of appetite, unbalanced swimming, expanded wounds, and petechia on the body surfaces, pectoral fins rot, visceral hemorrhage, bleeding on the spleen, and heart ascites were collected...
2018: Veterinary Research Forum
Alireza Yousefi, Farshid Sarrafzadeh-Rezaei, Siamak Asri-Rezaei, Amir-Abbas Farshid, Mehdi Behfar
Chitosan bears numerous properties, such as biocompatibility, biodegradability and non-toxicity making it suitable for use in different biomedical fields. Zinc (Zn) is required for fibroblasts proliferation and collagen synthesis as essential elements of wound healing. Its nanoparticles are well known for their capability to enhance wound healing by cell adhesion and migration improvement through growth factors-mediated mechanisms. Poor blood supply and unique histological characteristics of tendon make its regeneration always slow...
2018: Veterinary Research Forum
Parinaz Tavasolian, Masoud Rajabioun, Hamideh Salari Sedigh, Mohammad Azizzadeh
Ultrasonography is an accurate and accessible method for detecting polycystic kidney disease (PKD), an inherited autosomal dominant disease, and other urinary tract diseases. The present work is a preliminary study of PKD and urinary tract abnormalities using ultrasonography in Persian and other long hair cats in Iran. This study was conducted on 83 cats including 68 Persian cats and 15 Persian related cats from December 2013 to March 2015. The age of cats ranged 3 to 72 months. Cats were classified as PKD-positive when at least one renal cyst was observed...
2018: Veterinary Research Forum
Pouria Motamed Chaboki, Arash Ghalyanchilangeroudi, Vahid Karimi, Hamed Abdollahi, Hossein Maghsoudloo, Hossein Hosseini, Reza Khaltababdi Farahahni, Seyed Ali Ghafouri, Mohammad Hossein Falah, Hussein Rezaee, Masoumeh Jabbarifakhr, Fatemeh Sadat Mousavi
Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and swollen head syndrome in chickens. Four aMPV subgroups (A-D) have been reported previously based on their genetic and antigenic differences. Evidence suggests that the live bird markets (LBMs) play an important role in the epidemiology of the avian viral diseases. A total number of 450 oropharyngeal samples from eight different species of birds (migratory and local) were collected from LBMs of Gilan province, Iran, from October to December 2016...
2018: Veterinary Research Forum
Farkhondeh Pooyanfar, Tahereh Foroutan, Mojtaba Dashtizad
Although the rate of blastocysts implantation of embryos is higher than previous stages but their survival rate is lower than them, which could be attributed to the completely filled blastocoel cavity with liquid and increased possibility of the formation of ice crystals. This liquid could prevent the penetration of cryoprotecting materials into the embryos. In this study, we reduced the volume of blastocoel before vitrification and compared survival rate and quality of in vitro embryos through klf4 gene expression with control group...
2018: Veterinary Research Forum
Farnaz Malekifard, Minoo Ahmadpour
Giardia duodenalis is one of the most prevalent intestinal protozoa infecting humans and domestic animals. The aim of this study was to identify subspecies of G. duodenalis by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method from fecal samples of naturally infected cattle in the Urmia, West Azerbaijan province, Iran. Overall, 246 fecal specimens were collected from the cattle (diarrheic and healthy) and microscopically examined for G. duodenalis. The PCR-RFLP analysis of glutamate dehydrogenase (gdh) locus was used to identify the genotypes found in cattle...
2018: Veterinary Research Forum
Parviz Hassanzadeh, Mehran Moradi, Nasim Vaezi, Mir-Hassan Moosavy, Razzagh Mahmoudi
In recent years, use of edible coatings as carriers of food additives and antimicrobial compounds has been considered in fishery products. This study was carried out to evaluate the effects of 2.00% chitosan coating singly and combined with 0.10% grape seed extract (GSE) on microbial (mesophils and psychrophils counts), chemical (thiobarbituric acid; TBA), pH and peroxide value (PV) and sensorial properties of rainbow trout fillet stored at 4 ° C over a period of 15 days. The coating had a significant effect in reducing aerobic mesophilic and psychrophilic bacteria counts...
2018: Veterinary Research Forum
Masoud Reza Seyfi Abad Shapouri, Mohammad Rashno, Pezhman Mahmoodi, Mahshid Aria
Monoclonal antibodies (MAbs) are invaluable molecules which have several advantages over polyclonal immunoglobulins (Igs) including consistency and higher specificity and hence can be used in biological researches, diagnosis and treatment of diseases. The present study was conducted to produce monoclonal antibody against chicken IgG. The IgG molecules were purified from chicken serum and used as antigens to immunize several mice. Thereafter, a well-immunized mouse was chosen and used for fusion process. After production of hybridoma cells, several rounds of cloning were carried out and produced MAbs were examined by various immunological assays including enzyme-linked immunosorbent assay (ELISA) and western and dot blotting...
2018: Veterinary Research Forum
Neda Abedpour, Mojdeh Salehnia, Nassim Ghorbanmehr
Lysophosphatidic acid (LPA) known as a serum-derived growth factor, is involved in several cell physiological functions in the female reproductive system including: oocyte maturation, in vitro fertilization and embryo implantation by its transmembrane G protein-coupled receptors. The aim of the present study was to examine the effect of LPA on in vitro follicular development of mouse ovarian tissue. Neonatal mouse ovarian tissues were cultured in five different concentrations of LPA (0, 5, 10, 20 and 40 µM)...
2018: Veterinary Research Forum
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