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New Microbes and New Infections

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https://www.readbyqxmd.com/read/29234497/insertional-inactivation-of-oprd-in-carbapenem-resistant-pseudomonas-aeruginosa-strains-isolated-from-burn-patients-in-tehran-iran
#1
A Shariati, T Azimi, A Ardebili, A S Chirani, A Bahramian, A Pormohammad, M Sadredinamin, S Erfanimanesh, N Bostanghadiri, S Shams, A Hashemi
In this study, we report the insertion sequence ISPpu21 in the oprD porin gene of carbapenem-resistant Pseudomonas aeruginosa isolates from burn patients in Tehran, Iran. Antibiotic susceptibility tests for P. aeruginosa isolates were determined. Production of metallo-β-lactamases (MBLs) and carbapenemase was evaluated and the β-lactamase-encoding and aminoglycoside-modifying enzyme genes were investigated by PCR and sequencing methods. The mRNA transcription level of oprD and mex efflux pump genes were evaluated by real-time PCR...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29234496/persistent-diarrhoea-in-a-5-month-old-baby-carrying-vibrio-cholerae-nono1-nono139-producing-haitian-cholera-toxin
#2
P Kumar, S Karmakar, R Prasad, R Chopra, S Khandelwal, S Gupta, A C Dhariwal, P Yadav, P K Yadava
Cholera toxin (CT) is the principal virulence factor of Vibrio cholerae for fatal cholera diarrhoea. Serogroups O1 and O139 harbour CT and are known to be epidemic strains. The remaining serogroups (nonO1/nonO139) are non-toxigenic and may be associated with mild disease. O1 serogroup emerged with a variant of CT known as Haitian cholera toxin (HCT). The HCT strains are hypervirulent and have been associated with severe cholera outbreaks in India, Western Africa and Haiti. Here, we report the presence of HCT (ctxB7) in a nonO1/nonO139 isolate causing persistent diarrhoea...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204287/libanicoccus-massiliensis-gen-nov-sp-nov-a-new-bacterium-isolated-from-human-stool
#3
M Bilen, F Cadoret, M Richez, E Tomei, Z Daoud, D Raoult, P-E Fournier
Strain Marseille-P3237 was isolated from a stool sample of a healthy 35-year-old Congolese pygmy female. This anaerobic, Gram-negative, non-spore-forming and non-motile coccus-shaped bacterium is a member of the order Coriobacteriales. It exhibits a 2 009 306-bp genome with a 65.46 mol% G+C content and is closely related to, but distinct from, members of the Olsenella genus. We propose the creation of the new genus Libanicoccus gen. nov. and of the new species Libanicoccus massiliensis sp. nov.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204286/accurate-differentiation-of-escherichia-coli-and-shigella-serogroups-challenges-and-strategies
#4
REVIEW
N K Devanga Ragupathi, D P Muthuirulandi Sethuvel, F Y Inbanathan, B Veeraraghavan
Shigella spp. and Escherichia coli are closely related; both belong to the family Enterobacteriaceae. Phenotypically, Shigella spp. and E. coli share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant E. coli from Shigella spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204285/molecular-characterization-of-acinetobacter-baumannii-isolated-from-iraqi-hospital-environment
#5
I M S Al-Kadmy, A N M Ali, I M A Salman, S S Khazaal
Healthcare-associated items are a common source of acquired infections, and hospital-acquired infections cause significant mortality and morbidity worldwide. Acinetobacter baumannii is the most prevalent infection-causing organism in the hospital environment. Hospital articles and objects are the main sources of infection with the ability to transmit some of the pathogenic microorganisms such as A. baumannii, which is considered a serious problem in therapeutic treatments. In the current study, we isolated A...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204284/-brevibacterium-ihuae-sp-nov-isolated-from-a-stool-sample-of-a-healthy-25-year-old-woman
#6
C Valles, P-E Fournier, D Raoult, F Cadoret
We report the main characteristics of 'Brevibacterium ihuae' strain cv3T isolated from a stool sample of a healthy 25-year-old woman.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204283/genome-sequence-of-leucobacter-massiliensis-sp-nov-isolated-from-human-pharynx-after-travel-to-the-2014-hajj
#7
T Leangapichart, P Gautret, T T Nguyen, N Armstrong, J-M Rolain
"Leucobacter massiliensis" strain 122RC15T sp. nov. is a new species within the genus Leucobacter. The genome of this strain is described here. It was isolated from the pharynx of a 76-year-old Algerian female after travelling from the 2014 Hajj. "Leucobacter massiliensis" is a Gram-positive, aerobic bacillus. Here we describe the features including complete genome and annotation of this strain. The 3 136 406-bp long genome contains 2797 protein-coding genes and 49 RNA genes.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204282/in%C3%A2-vivo-selection-of-a-multidrug-resistant-aeromonas-salmonicida-during-medicinal-leech-therapy
#8
E Ruppé, A Cherkaoui, N Wagner, G C La Scala, J-Y Beaulieu, M Girard, J Frey, V Lazarevic, J Schrenzel
We report the selection in a 15-year-old boy of a multidrug-resistant, extended-spectrum β-lactamase (ESBL)-producing Aeromonas salmonicida after medicinal leech therapy that required an antibiotic prophylaxis based on piperacillin/tazobactam and cotrimoxazole. Whole genome sequencing of the strain indeed revealed 13 antibiotic resistance genes, including the ESBL CTX-M-3 and the unusual β-lactamase SCO-1.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29204281/-lactobacillus-raoultii-sp-nov-a-new-bacterium-isolated-from-the-vaginal-flora-of-a-woman-with-bacterial-vaginosis
#9
B Nicaise, M Bilen, F Cadoret, F Bretelle, F Fenollar
We report the isolation of a new bacterium species, 'Lactobacillus raoultii' strain Marseille P4006 (CSUR P4006), isolated from a vaginal sample of a 45-year-old woman with bacterial vaginosis.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29201381/culture-independent-genome-sequencing-of-coxiella-burnetii-from-a-native-heart-valve-of-a-tunisian-patient-with-severe-infective-endocarditis
#10
J Delaloye, T Pillonel, M Smaoui, A Znazen, L Abid, G Greub
We report draft genome of a Coxiella burnetii strain sequenced from the native valve of a patient presenting with severe endocarditis in Tunisia. The genome could be sequenced without a cellular or axenic culture step. The MST5 strain was demonstrated to be closely related to the published reference genome of C. burnetii CbuK_Q154.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29201380/first-case-of-bacteraemia-due-to-acinetobacter-schindleri-harbouring-blandm-1-in-an-immunocompromised-patient
#11
S Montaña, S Palombarani, M Carulla, A Kunst, C H Rodriguez, M Nastro, C Vay, M S Ramirez, M Almuzara
Clinically significant NDM-1-producing Acinetobacter schindleri has not yet been described in the literature. We report the first case of bacteraemia due to an A. schindleri strain harbouring blaNDM-1 recovered from an immunocompromised patient. Our report reinforces the fact that NDM-1 can easily be acquired by Acinetobacter species.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29188064/-streptomyces-massilialgeriensis-sp-nov-a-new-bacterial-species-isolated-from-an-extremely-saline-soil-collected-from-the-dry-lake-of-ank-el-djamel-in-algeria
#12
C E Djaballah, M Kitouni, D Raoult, S Khelaifia
We report here the main characteristics of "Streptomyces massilialgeriensis" strain S35T (CSUR = P3927), a new bacterial species within the Streptomyces genus, isolated from an extremely saline soil sample collected from the site of Garaet Ank Djemel in the Wilaya of Oum El Bouaghi, Algeria.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29188063/high-rates-of-nontuberculous-mycobacteria-isolation-from-patients-with-presumptive-tuberculosis-in-iran
#13
M J Nasiri, H Dabiri, A A I Fooladi, S Amini, G Hamzehloo, M M Feizabadi
Nontuberculous mycobacteria (NTM) can cause disease which can be indistinguishable from tuberculosis (TB), posing a diagnostic and therapeutic challenge, particularly in low- and middle-income settings. We aimed to investigate the mycobacterial agents associated with presumptive clinical pulmonary TB in Iran. A total of 410 mycobacterial isolates, obtained between March 2014 and January 2016, from 7600 clinical samples taken from consecutive cases of presumptive diagnosis of TB were identified. Phenotypic and molecular tests were used to identify the isolated organisms to the species level...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158909/first-complete-genome-sequence-of-circulating-dengue-virus-serotype-3-in-jeddah-saudi-arabia
#14
A M Hashem, S S Sohrab, S A El-Kafrawy, S A El-Ela, A M M Abd-Alla, S A Farraj, N A Othman, A M Hassan, M M El-Daly, R N Charrel, T A Madani, E I Azhar
Here we report the first full-length genome sequence of dengue virus serotype 3 (DENV-3) from a strain isolated from a patient in Jeddah, Saudi Arabia, in 2014. The genome consists of 10 635 bp and shows close similarity to circulating genotype III isolates from Singapore, suggesting possible importation, most probably during religious pilgrimages to Saudi Arabia.
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29071070/-massilibactero%C3%A3-des-vaginae-gen-nov-sp-nov-a-new-genus-within-the-family-of-bactero%C3%A3-daceae-isolated-from-a-patient-with-vaginosis
#15
J Jneid, J Y Bou Khalil, S Aherfi, C Blanc-Tailleur, D Raoult, B La Scola, I Pagnier
'Massilibacteroïdes vaginae' sp. nov. strain MV12(T) is the type strain of 'Massilibacteroïdes' gen. nov., a new genus within the family of Porphyromonadaceae. This strain was isolated in Marseille from a patient with vaginosis. 'Massilibacteroïdes vaginae' is a Gram-negative, aero-anaerobic bipolar bacillus. Here we describe the phenotypic characteristics and complete genome sequence of this bacterium. The EMBL accession number is FXDJ00000000 (FXDJ01000001-FXDJ01000009), and its genome consists of nine scaffolds...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158908/dairy-production-practices-and-associated-risks-for-bovine-vaccinia-exposure-in-cattle-brazil
#16
I A Borges, A M McCollum, J M Mehal, D Haberling, L A L Dutra, F N Vieira, L A O Andrade, E G Kroon, R C Holman, M G Reynolds, G S Trindade
A cross-sectional serosurvey was performed to identify environmental features or practices of dairy farms associated with risk for exposure to vaccinia-like viruses in dairy cattle in Brazil. Sera from 103 cows from 18 farms in Minas Gerais state were examined for Orthopoxvirus-neutralizing antibodies. A database of 243 binary or multiple-selection categorical variables regarding the physical features and surrounding ecology of each property was obtained. Thirteen of 46 presumptive predictor variables were found to be significantly associated with Orthopoxvirus serostatus by univariate logistic regression methods...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158907/chikungunya-outbreak-in-delhi-india-2016-report-on-coinfection-status-and-comorbid-conditions-in-patients
#17
N Kaur, J Jain, A Kumar, M Narang, M K Zakaria, A Marcello, D Kumar, R Gaind, S Sunil
Chikungunya fever is a major public health issue in India affecting billions. After 2010, the infection was in a decline until in 2016, when a massive outbreak affected the country. In this report, we present serologic and molecular investigations of 600 patient samples for chikungunya and dengue viruses along with clinical and comorbidity features. We recruited 600 patients during this outbreak and evaluated them for chikungunya and dengue virus antibodies and virus RNA through IgM, NS1 antigen and quantitative real-time PCR (qPCR)...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158906/molecular-characterization-of-panton-valentine-leukocidin-pvl-toxin-encoding-phages-from-south-india
#18
V Balaji, D B Yamuna, Y I Francis, G Priya Doss
A total of 19 methicillin-resistant Staphylococcus aureus (MRSA) isolates were investigated for Panton-Valentine leukocidin (PVL) toxin, PVL gene sequence variation and PVL-encoding phages. Whole genome sequencing was performed for all isolates. Analysis of MRSA isolates (n = 19) confirmed that most MRSA (n = 11) were positive for the PVL gene and were multidrug resistant. ST772-MRSA-V was the predominant PVL-positive MRSA clone, although all of them were found to carry the ΦIND772PVL phage in the genome...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158905/genome-sequence-and-description-of-mobilicoccus-massiliensis-sp-nov-isolated-from-the-stool-of-a-nigerian-boy-with-kwashiorkor
#19
N Mathlouthi, S I Traore, T Cimmino, S Khelaifia, T T Nguyen, F Cadoret, C Couderc, D Raoult, J-M Rolain
Mobilicoccus massiliensis strain SIT2 (= CSUR P1306 = DSM 29065) is a new type strain of Mobilicoccus sp. nov. isolated from the stool of a 2-year-old Nigerian boy with kwashiorkor. M. massiliensis is Gram positive, facultatively anaerobic, nonsporulating and motile. The 3 842 438 bp long genome contains 3362 protein-coding and 49 RNA genes, including one 5S rRNA gene, one 16S rRNA gene, one 23S rRNA gene and 46 tRNA genes.
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158904/follow-up-and-report-on-active-trachoma-in-zabol-iran-prompted-by-sharifi-rad-and-fallah-s-observations-published-in-may-2016
#20
S-F Mohammadi, A Bazi-Shad, J Akbarizadeh, E Ashrafi, Z Moinfar, E Shirdel, M Nabavi, S Samiei, S Salehi-Rad, A W Solomon
No abstract text is available yet for this article.
November 2017: New Microbes and New Infections
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