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Plant Pathology Journal

Nam-Yeon Kim, Jonghee Oh, Su-Heon Lee, Hongsup Kim, Jae Sun Moon, Rae-Dong Jeong
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C...
December 2018: Plant Pathology Journal
Min-Jeong Kim, Chang-Ki Shim, Yong-Ki Kim, Byong-Gu Ko, Jong-Ho Park, Soo-Gen Hwang, Baeg-Ho Kim
This study was conducted to investigate the efficacy of freshwater alga, Chlorella fusca on the improvement of growth and qualities in organic spinach and Chinese chives farm. The average height of Chinese chives treated with the chlorella was 3.7 cm smaller than that of the untreated. The leaf width and fresh weight of Chinese chives treated with the chlorella was 0.5 mm wider and 30.3 g heavier than that of the untreated. The commercialization and yield of Chinese chives treated with the chlorella was 11...
December 2018: Plant Pathology Journal
Young Hee Lee, Su Jeong Jang, Joon-Hee Han, Jin Su Bae, Hyunsuk Shin, Hee Jin Park, Mee Kyung Sang, Song Hee Han, Kyoung Su Kim, Sang-Wook Han, Jeum Kyu Hong
Two rhizobacteria Bacillus aryabhattai H26-2 and B. siamensis H30-3 were evaluated whether they are involved in stress tolerance against drought and high temperature as well as fungal infections in Chinese cabbage plants. Chinese cabbage seedlings cv. Ryeokgwang (spring cultivar) has shown better growth compared to cv. Buram-3-ho (autumn cultivar) under high temperature conditions in a greenhouse, whilst there was no difference in drought stress tolerance of the two cultivars. In vitro growth of B. aryabhattai H26-2 and B...
December 2018: Plant Pathology Journal
Seloame Tatu Nyaku, Hanif Lutuf, Eric Cornelius
Tomato ( Solanum lycopersicum ) production in Ghana is limited by the root-knot nematode ( Meloidogyne incognita , and yield losses over 70% have been experienced in farmer fields. Major management strategies of the root-knot nematode (RKN), such as rotation and nematicide application, and crop rotation are either little efficient and harmful to environments, with high control cost, respectively. Therefore, this study aims to examine morphometric variations of RKN populations in Ghana, using principal component analysis (PCA), of which the information can be utilized for the development of tomato cultivars resistant to RKN...
December 2018: Plant Pathology Journal
Hae-Ryun Kwak, Hee Ju Lee, Eun-A Kim, Jang-Kyun Seo, Chang-Seok Kim, Sang Gyu Lee, Jeong-Soo Kim, Hong-Soo Choi, Mikyeong Kim
Complete genome sequences of 22 isolates of Cucurbit aphid-borne yellows virus (CABYV), collected from melon plants showing yellowing symptom in Korea during the years 2013-2014, were determined and compared with previously reported CABYV genome sequences. The complete genomes were found to be 5,680-5,684 nucleotides in length and to encode six open reading frames (ORFs) that are separated into two regions by a non-coding internal region (IR) of 199 nucleotides. Their genomic organization is typical of the genus Polerovirus ...
December 2018: Plant Pathology Journal
Mahsa Abadkhah, Davoud Koolivand, Omid Eini
Tomato spotted wilt virus (TSWV; Genus Orthotospovirus : Family Tospoviridae ) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato ( Solanum lycopersicum ) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively...
December 2018: Plant Pathology Journal
Ji-Yun Jeong, Arif Hasan Khan Robin, Sathishkumar Natarajan, Rawnak Laila, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou
Clubroot is one of the most economically important diseases of the Brassicaceae family. Clubroot disease is caused by the obligate parasite Plasmodiophora brassicae , which is difficult to study because it is non-culturable in the laboratory and its races are genetically variable worldwide. In Korea, there are at least five races that belongs to four pathotype groups. A recent study conducted in Korea attempted to develop molecular markers based on ribosomal DNA polymorphism to detect P. brassicae isolates, but none of those markers was either race-specific or pathotype-specific...
December 2018: Plant Pathology Journal
Cheol Woo Choi, Jae Wook Hyun, Rok Yeon Hwang, Charles A Powell
Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting gene contained tandem repeat for more rapid and sensitive detection of Candidatus Liberibacter asiaticus ( C Las), putative causal agent of the citrus huanglongbing...
December 2018: Plant Pathology Journal
Chaeyeong Lee, Hyun-Hee Lee, Mohamed Mannaa, Namgyu Kim, Jungwook Park, Juyun Kim, Young-Su Seo
Panicle blight and seed rot disease caused mainly by Burkholderia glumae and Burkholderia gladioli is threatening rice cultivation worldwide. The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli . The designed primers were specific in detecting the target species with no apparent crossreactions with other related Burkholderia species at the expected product size...
December 2018: Plant Pathology Journal
May Moe Oo, Ha-Yeon Yoon, Hyun A Jang, Sang-Keun Oh
Bitter rot caused by Colletotrichum species is a common fruit rotting disease of apple and one of the economically important disease in worldwide. In 2015 and 2016, distinct symptoms of bitter rot disease were observed in apple orchards in five regions of South Korea. In the present study, infected apples from these regions were utilized to obtain eighteen isolates of Colletotrichum spp. These isolates were identified and characterized according to their morphological characteristics and nucleotide sequence data of internal transcribed spacer regions and glyceraldehyde-3-phosphate-dehydrogenase...
December 2018: Plant Pathology Journal
Teng Fu, Joon-Oh Kim, Joon-Hee Han, Adiyantara Gumilang, Yong-Hwan Lee, Kyoung Su Kim
The rice blast pathogen Magnaporthe oryzae is a global threat to rice production. Here we characterized RHO2 gene ( MGG_02457 ) that belongs to the Rho GTPase family, using a deletion mutant. This mutant ΔMorho2 exhibited no defects in conidiation and germination but developed only 6% of appressoria in response to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the mutant led to appressoria that exhibited abnormal morphology on both hydrophobic and hydrophilic surfaces...
December 2018: Plant Pathology Journal
Rouhallah Sharifi, Choong-Min Ryu
Plants and microorganisms (microbes) use information from chemicals such as volatile compounds to understand their environments. Proficiency in sensing and responding to these infochemicals increases an organism's ecological competence and ability to survive in competitive environments, particularly with regard to plant-pathogen interactions. Plants and microbes acquired the ability to sense and respond to biogenic volatiles during their evolutionary history. However, these signals can only be interpreted by humans through the use of state-of the-art technologies...
December 2018: Plant Pathology Journal
Hae-Ryun Kwak, Jaedeok Kim, Mikyeong Kim, Jang-Kyun Seo, Jeong-Soo Kim, Hong-Soo Choi
The Sweet potato chlorotic fleck virus (SPCFV), of the genus Carlavirus (family Betaflexiviridae), was first detected as one of several viruses infecting sweet potatoes ( Ipomea batatas L.) in Korea. Out of 154 sweet potato samples collected in 2012 that were showing virus-like symptoms, 47 (31%) were infected with SPCFV, along with other viruses. The complete genome sequences of four SPCFV isolates were determined and analyzed using previously reported genome sequences. The complete genomes were found to contain 9,104-9,108 nucleotides, excluding the poly-A tail, containing six putative open reading frames (ORFs)...
October 2018: Plant Pathology Journal
Jungkum Park, Gyu Min Lee, Donghyuk Kim, Duck Hwan Park, Chang-Sik Oh
Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. Erwinia amylovora and E. pyrifoliae cause fire blight and black shoot blight disease in apple and pear, respectively. In this study, the bacteriophage phiEaP-8 was isolated from apple orchard soil and could efficiently and specifically kill both E. amylovora and E. pyrifoliae . This bacteriophage belongs to the Podoviridae family. Whole genome analysis revealed that phiEaP-8 carries a 75,929 bp genomic DNA with 78 coding sequences and 5 tRNA genes...
October 2018: Plant Pathology Journal
Won-Hee Kang, Seon-In Yeom
Receptor-like proteins (RLPs) are involved in plant development and disease resistance. Only some of the RLPs in tomato ( Solanum lycopersicum L.) have been functionally characterized though 176 genes encoding RLPs, which have been identified in the tomato genome. To further understand the role of RLPs in tomato, we performed genome-guided classification and transcriptome analysis of these genes. Phylogenic comparisons revealed that the tomato RLP members could be divided into eight subgroups and that the genes evolved independently compared to similar genes in Arabidopsis...
October 2018: Plant Pathology Journal
M H Ahmad, M T Shakeel, I M Al-Shahwan, M A Al-Saleh, M A Amer
During the spring season of 2014, a total of 148 melon and watermelon leaf samples were collected from symptomatic and asymptomatic plants in the western and southwestern regions of Saudi Arabia and were tested for the presence of Watermelon chlorotic stunt virus (WmCSV) and other suspected cucurbit viruses by double antibody sandwich enzyme-linked immunosorbent assays. Ninety-eight samples were found to be positive for the presence of WmCSV, nine samples were positive for the presence of Cucurbit yellow stunting disorder virus (CYSDV), and 22 showed a mixed infection with both WmCSV and CYSDV...
October 2018: Plant Pathology Journal
Jieun Kim, Mohamed Mannaa, Namgyu Kim, Chaeyeong Lee, Juyun Kim, Jungwook Park, Hyun-Hee Lee, Young-Su Seo
The Hfq protein is a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was conducted to elucidate the functions of two hfq genes in Burkholderia glumae , a causal agent of rice grain rot. Therefore, mutant strains of the rice-pathogenic B. glumae BGR1, targeting each of the two hfq genes, as well as the double defective mutant were constructed and tested for several phenotypic characteristics...
October 2018: Plant Pathology Journal
Xiaoran Wang, Junfeng Shi, Rufu Wang
This study takes strawberry-fruits as the test material and discusses the effect of Burkholderia contaminans B-1 on preventing postharvest diseases and inducing resistance-related substances in strawberry-fruits. Soaking and wound inoculating is performed to analyze the inhibitory effects of different treatment solutions on the gray mold of postharvest strawberry-fruits. The count of antagonistic bacteria colonies in the wound is found, and the dynamic growth of antagonistic bacteria and the pathogenic fungus is observed by electron microscopy...
October 2018: Plant Pathology Journal
Luz Adriana Pedraza, Jessica Bautista, Daniel Uribe-Vélez
Rice world production is affected due to the growing impact of diseases such as bacterial panicle blight, produced by Burkholderia glumae . The pathogen-induced symptoms include seedling rot, grain rot and leaf-sheath browning in rice plants. It is currently recognized the entrance of this pathogen to the plant, from infected seeds and from environmental sources of the microorganism. However, it is still not fully elucidated the dynamics and permanence of the pathogen in the plant, from its entry until the development of disease symptoms in seedlings or panicles...
October 2018: Plant Pathology Journal
Moisés Roberto Vallejo Pérez, Hugo Ricardo Navarro Contreras, Jesús A Sosa Herrera, José Pablo Lara Ávila, Hugo Magdaleno Ramírez Tobías, Fernando Díaz-Barriga Martínez, Rogelio Flores Ramírez, Ángel Gabriel Rodríguez Vázquez
Clavibacter michiganensis subsp. michiganesis ( Cmm ) is a quarantine-worthy pest in México. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm , the disease epidemiology was monitored. Micro-Raman spectroscopy (532 nm λ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint...
October 2018: Plant Pathology Journal
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