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https://www.readbyqxmd.com/read/28203534/comparison-of-methanol-and-isopropanol-as-wash-solvents-for-determination-of-hair-cortisol-concentration-in-grizzly-bears-and-polar-bears
#1
Thomas Kroshko, Luciene Kapronczai, Marc R L Cattet, Bryan J Macbeth, Gordon B Stenhouse, Martyn E Obbard, David M Janz
Methodological differences among laboratories are recognized as significant sources of variation in quantification of hair cortisol concentration (HCC). An important step in processing hair, particularly when collected from wildlife, is the choice of solvent used to remove or "wash" external hair shaft cortisol prior to quantification of HCC. The present study systematically compared methanol and isopropanol as wash solvents for their efficiency at removing external cortisol without extracting internal hair shaft cortisol in samples collected from free-ranging grizzly bears and polar bears...
2017: MethodsX
https://www.readbyqxmd.com/read/28203533/modification-of-extraction-method-for-community-dna-isolation-from-salt-affected-compact-wasteland-soil-samples
#2
Purvi Zaveri, Rushika Patel, Meghavi Patel, Devki Sarodia, Nasreen S Munshi
To overcome the issue of interferences by salt and compactness in release of bacterial cell required for lysis, method described by Yeates et al. (1998), was optimized for isolation of genomic material (Deoxyribo Nucleic Acid, DNA) from soil microbial community by addition of Al(NH4)SO4. Very low total viable count was observed in the samples tested and hence use of higher amount of soil is required primarily for DNA isolation from wasteland soils. The method proves itself efficient where commercially available bead beating and enzymatic lysis methods could not give isolation of any amount of community genomic DNA due to compact nature and salt concentrations present in soil...
2017: MethodsX
https://www.readbyqxmd.com/read/28138433/organic-matter-from-benthic-foraminifera-ammonia-beccarii-shells-by-ft-ir-spectroscopy-a-study-on-tupilipalem-south-east-coast-of-india
#3
G Sreenivasulu, N Jayaraju, B C Sundara Raja Reddy, T Lakshmi Prasad, K Nagalakshmi, B Lakshmanna
Fourier Transform Infrared Spectroscopy (FTIR) was used to study the variations in organic matters of benthic foraminifera (Ammonia beccarii) from four samples collected from beach environments from brackish environments along Tupilipalem coast (South east coast of India). Common absorption bands were observed as peaks in the range of 3600-3400 cm(-1), 3000-2850 cm(-1), 1750-1740 cm(-1), 1640-1600 cm(-1), 1450-1350 cm(-1), 885-870 cm(-1) and 725-675 cm(-1) in all the shells of Ammonia beccarii. The FTIR spectrum of station-1 represents the presence of alkanes (CH3) and alkyl halide (C-F stretching) with absorptions at the range 1385-1255 and 1350-1150 cm(-1) were observed and ether (C-O stretching) absorption band was observed at stations 1 and 3 with wavenumber of 1115 cm(-1) and 1117 cm(-1) respectively...
2017: MethodsX
https://www.readbyqxmd.com/read/28119824/precision-measurement-of-radioactivity-in-gamma-rays-spectrometry-using-two-hpge-detectors-bege-6530-and-gc0818-7600sl-models-comparison-techniques-application-to-the-soil-measurement
#4
Guembou Shouop Cebastien Joel, Samafou Penabei, Moyo Maurice Ndontchueng, Gregoire Chene, Eric Jilbert Nguelem Mekontso, Alexandre Ngwa Ebongue, Motapon Ousmanou, Strivay David
To obtain high quality of results in gamma spectrometry, it is necessary to select the best HPGe detector for particular measurements, to calibrate energy and efficiency of gamma detector as accurate as possible. To achieve this aim, the convenient detector model and gamma source can be very useful. The purpose of this study was to evaluate the soil specific activity using two HPGe model (BEGe-6530 and GC0818-7600SL) by comparing the results of the two detectors and the technics used according to the detector type...
2017: MethodsX
https://www.readbyqxmd.com/read/28116247/understanding-the-decay-of-proteins-a-method-to-study-time-dependent-response-of-pm-concentration-of-insulin-at-microwave-frequencies
#5
Ritika Verma, K S Daya
Bio-molecule when isolated from its natural ecological condition is subjected to rapid decay. This decay leads to change in polarization and permittivity of molecule. This study presents an experimental analysis of the decay pattern of pM concentration of insulin using whispering gallery mode (WGM) dielectric resonator (DR) method. Analysis is carried out by comparing the permittivity, relaxation time and time delay for three days. It is observed that different pM concentrations of insulin solutions start to decay after 24 h at 5°C...
2017: MethodsX
https://www.readbyqxmd.com/read/28116246/logistic-regression-analysis-of-populations-of-electrophysiological-models-to-assess-proarrythmic-risk
#6
Stefano Morotti, Eleonora Grandi
Population-based computational approaches have been developed in recent years and helped to gain insight into arrhythmia mechanisms, and intra- and inter-patient variability (e.g., in drug responses). Here, we illustrate the use of multivariable logistic regression to analyze the factors that enhance or reduce the susceptibility to cellular arrhythmogenic events. As an example, we generate 1000 model variants by randomly modifying ionic conductances and maximal rates of ion transports in our atrial myocyte model and simulate an arrhythmia-provoking protocol that enhances early afterdepolarization (EAD) proclivity...
2017: MethodsX
https://www.readbyqxmd.com/read/28070500/chimeric-influenza-haemagglutinins-generation-and-use-in-pseudotype-neutralization-assays
#7
Francesca Ferrara, Nigel Temperton
Recently chimeric influenza haemagglutinins (cHAs) have been generated as potential 'universal' vaccination antigens and as tools to identify HA stalk-directed antibodies via their use as antigens in ELISA, and virus or pseudotype-based neutralization assays. The original methods [1], [2] used for their generation require the amplification of regions of interest (head and stalk) using primers containing SapI sites and subsequent cloning into pDZ plasmid. This requires precise primer design, checking for the absence of SapI sites in the sequence of interest, and multi-segment ligation...
2017: MethodsX
https://www.readbyqxmd.com/read/28053880/adapting-axelrod-s-cultural-dissemination-model-for-simulating-peer-effects
#8
Christian Hofer, Gernot Lechner, Thomas Brudermann, Manfred Füllsack
We present a generic method for considering incomplete but gradually expandable sociological data in agent-based modeling based on the classic model of cultural dissemination by Axelrod. Our method extension was inspired by research on the diffusion of citizen photovoltaic initiatives, i.e. by initiatives in which citizens collectively invest in photovoltaic plants and share the profits. Owing to the absence of empirical interaction parameters, the Axelrod model was used as basis for considering peer effects with contrived interaction data that can be updated from empirical surveys later on...
2017: MethodsX
https://www.readbyqxmd.com/read/28003965/a-method-to-implement-the-reservoir-wave-hypothesis-using-phase-contrast-magnetic-resonance-imaging
#9
Robert D M Gray, Kim H Parker, Michael A Quail, Andrew M Taylor, Giovanni Biglino
The reservoir-wave hypothesis states that the blood pressure waveform can be usefully divided into a "reservoir pressure" related to the global compliance and resistance of the arterial system, and an "excess pressure" that depends on local conditions. The formulation of the reservoir-wave hypothesis applied to the area waveform is shown, and the analysis is applied to area and velocity data from high-resolution phase-contrast cardiovascular magnetic resonance (CMR) imaging. A validation study shows the success of the principle, with the method producing largely robust and physically reasonable parameters, and the linear relationship between flow and wave pressure seen in the traditional pressure formulation is retained...
2016: MethodsX
https://www.readbyqxmd.com/read/27942465/longqt-a-cardiac-electrophysiology-simulation-platform
#10
Birce Onal, Daniel Gratz, Thomas Hund
Mathematical modeling has been used for over half a century to advance our understanding of cardiac electrophysiology and arrhythmia mechanisms. Notably, computational studies using mathematical models of the cardiac action potential (AP) have provided important insight into the fundamental nature of cell excitability, mechanisms underlying both acquired and inherited arrhythmia, and potential therapies. Ultimately, an approach that tightly integrates mathematical modeling and experimental techniques has great potential to accelerate discovery...
2016: MethodsX
https://www.readbyqxmd.com/read/27924282/a-computationally-efficient-algorithm-for-fitting-ion-channel-parameters
#11
Zachary R Teed, Jonathan R Silva
Continuous time Markov models have been widely used to describe ion channel kinetics, providing explicit representation of channel states and transitions. Fitting models to experimental data remains a computationally demanding task largely due to the high cost of model evaluation. Here, we propose a method to efficiently optimize model parameters and structure. Voltage clamp channel protocols can be decomposed into a series of fixed steps of constant voltage resulting in a set of linear systems of differential equations...
2016: MethodsX
https://www.readbyqxmd.com/read/27872822/automating-hesc-differentiation-with-3d-printing-and-legacy-liquid-handling-solutions
#12
Eric Zluhan, Kathleen Kelly, Nick LeClair, Danique Wortel, Kesley Moody
Historically, the routine use of laboratory automation solutions has been prohibitively expensive for many laboratories. As legacy hardware has begun to emerge on the secondary market, automation is becoming an increasingly affordable option to augment workflow in virtually any laboratory. To assess the utility of legacy liquid handling in stem cell differentiation, a used liquid handling robot was purchased at auction to automate a stem cell differentiation protocol that gives rise to CD14 + CD45+ mononuclear cells...
2016: MethodsX
https://www.readbyqxmd.com/read/27822451/so-much-data-so-little-time-using-sequential-data-analysis-to-monitor-behavioral-changes
#13
Tywanquila Walker
Twenty-three infants (M = 13.7 months, SD = 3.73) and their primary caregivers were observed and video-taped in three 20-min play sessions. Over the course of a month, changes in infant behaviors and caregiver responsiveness to those behaviors were monitored. Repeated-measures ANOVAs indicated that caregiver responsiveness to infant object-related and dyadic behaviors significantly increased over the course of the sessions. However, the ANOVAs did not specify exactly which caregiver behaviors changed. Sequential data analysis revealed that caregivers specifically increased their use of dyadic vocal behaviors in response to all infant behaviors...
2016: MethodsX
https://www.readbyqxmd.com/read/27822450/a-simple-and-effective-method-for-ultrastructural-analysis-of-mitosis-in-drosophila-s2-cells
#14
Anton Strunov, Lidiya V Boldyreva, Gera A Pavlova, Alexey V Pindyurin, Maurizio Gatti, Elena Kiseleva
The Drosophila S2 tissue culture cells are a widely used system for studies on mitosis. S2 cells are particularly sensitive to gene silencing by RNA interference (RNAi), allowing targeted inactivation of mitotic genes. S2 cells are also well suited for high-resolution light microscopy analysis of mitosis in fixed cells, and can be easily immunostained to detect mitotic components. In addition, S2 cells are amenable to transformation with plasmid encoding fluorescently tagged mitotic proteins, allowing in vivo analysis of their behavior throughout cell division...
2016: MethodsX
https://www.readbyqxmd.com/read/27818948/fabrication-and-characterization-of-carbon-backed-thin-208-pb-targets
#15
Meenu Thakur, R Dubey, S R Abhilash, B R Behera, B P Mohanty, D Kabiraj, Sunil Ojha, Heena Duggal
Thin carbon-backed isotopically enriched (208)Pb targets were required for our experiment aimed to study the reaction dynamics for (48)Ti + (208)Pb system, populating the near super-heavy nucleus (256)Rf, through mass-energy correlation of the fission fragments. Purity and thickness of the targets are of utmost importance in such studies as these factors have strong influence on the measurement accuracy of mass and energy distribution of fission fragments. (208)Pb targets with thickness ranging from 60 μg/cm(2) to 250 μg/cm(2) have been fabricated in high vacuum environment using physical vapor deposition method...
2016: MethodsX
https://www.readbyqxmd.com/read/27774412/100%C3%A2-hz-neutron-radiography-at-the-boa-beamline-using-a-parabolic-focussing-guide
#16
Pavel Trtik, Manuel Morgano, Roman Bentz, Eberhard Lehmann
The recent developments in scientific complementary metal oxide semiconductor (sCMOS) detector technology allow for imaging of relevant processes with very high temporal resolution with practically negligible readout time. However, it is neutron intensity that limits the high temporal resolution neutron imaging. In order to partially overcome the neutron intensity problem for the high temporal resolution imaging, a parabolic neutron focussing guide was utilized in the test arrangement and placed upstream the detector in such a manner that the focal point of the guide was positioned slightly behind the scintillator screen...
2016: MethodsX
https://www.readbyqxmd.com/read/27766212/vacuum-system-upgrade-for-extended-q-range-small-angle-neutron-scattering-diffractometer-eq-sans-at-sns
#17
Christopher Stone, Derrick Williams, Jeremy Price
The Extended Q-range Small-angle Neutron Scattering Diffractometer (EQ-SANS) instrument at the spallation neutron source (SNS), Oak Ridge, Tennessee, incorporates a 69 m(3) detector vessel with a vacuum system which required an upgrade with respect to performance, ease of operation, and maintenance. The upgrade focused on improving pumping performance as well as optimizing system design to minimize opportunity for operational error. This upgrade provided the following practical contributions: •Reduced time required to evacuate from atmospheric pressure to 2 mTorr from 500 to 1000 min to 60-70 min•Provided turn-key automated control with a multi-faceted interlock for personnel and machine safety...
2016: MethodsX
https://www.readbyqxmd.com/read/27709068/bquant-novel-script-for-batch-quantification-of-lcms-data
#18
Marko Rožman, Mira Petrović
Quantitative target analysis by liquid chromatography coupled to mass spectrometry (LCMS) is ubiquitous in environmental, metabolomic and toxicological studies. Targeted LCMS methods are capable of the simultaneous determination of literally hundreds of analytes. Although acquiring of instrumental data is very fast, data post-processing i.e. quantification can be time consuming step (and)or dependent to various commercial software packages. In attempt to facilitate this drawback Wolfram Mathematica script for batch quantification of LCMS data was created...
2016: MethodsX
https://www.readbyqxmd.com/read/27630821/direct-field-method-for-root-biomass-quantification-in-agroecosystems
#19
Ileana Frasier, Elke Noellemeyer, Romina Fernández, Alberto Quiroga
The present article describes a field auger sampling method for row-crop root measurements. In agroecosystems where crops are planted in a specific design (row crops), sampling procedures for root biomass quantification need to consider the spatial variability of the root system. This article explains in detail how to sample and calculate root biomass considering the sampling position in the field and the differential weight of the root biomass in the inter-row compared to the crop row when expressing data per area unit...
2016: MethodsX
https://www.readbyqxmd.com/read/27617231/use-of-cross-reactivity-immunoassay-to-orient-insulin-replacement-in-diabetic-patients-with-high-levels-of-insulin-antibodies
#20
Alejandro Cardoso Landaburu, María Pomares, Alfredo Avalos, Silvia Lapertosa, Gustavo Frechtel, Edgardo Poskus
The prevalence and high levels of anti-insulin antibodies (IA) have frequently been associated with brittle diabetes, lipodystrophy in the areas where the insulin is injected and/or poor metabolic control. When this happens the usual criterion adopted is the empirical change of insulin type and/or formulation intending to diminish the IA level and then to decrease the undesirable side-effects. Here, we present a rational two step radiometric method consisting in: A) a first-line radioligand binding assay (RBA) to assess IA in sera of these patients and detecting those with high levels...
2016: MethodsX
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