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Biotechnology Reports

Ratana Chaiklahan, Nattayaporn Chirasuwan, Veara Loha, Suvit Tia, Boosya Bunnag
Arthrospira ( Spirulina ) consists of diverse high-value chemicals, such as phycocyanin, lipids/total fatty acids (TFA), and polysaccharides, which have been used for food, cosmetic and pharmacological applications. This study compared various stepwise extraction processes for these high-value chemicals. Considering the yield and properties of extracts, the most suitable extraction order was phycocyanin, lipid/TFA and polysaccharides. The yield of the main product (food-grade phycocyanin) was 8.66% of the biomass dry weight, whereas the yields of the subsequent lipid/TFA and polysaccharide coproducts were 3...
December 2018: Biotechnology Reports
Silvia Hüttner, Zoraide Granchi, Thanh Thuy Nguyen, Sake van Pelt, Johan Larsbrink, Vu Nguyen Thanh, Lisbeth Olsson
We report here the annotated draft genome sequence of the thermophilic zygomycete Rhizomucor pusillus strain FCH 5.7, isolated from compost soil in Vietnam. The genome assembly contains 25.59 Mb with an overall GC content of 44.95%, and comprises 10,898 protein coding genes. Genes encoding putative cellulose-, xylan- and chitin-degrading proteins were identified, including two putative endoglucanases (EC from glycoside hydrolase family 9, which have so far been mostly assigned to bacteria and plants...
December 2018: Biotechnology Reports
Zahid Ur Rehman, Intikhab Alam, Allan Anthony Kamau, Vladimir B Bajic, TorOve Leiknes
With antimicrobial resistance on the rise, the discovery of new compounds with novel structural scaffolds exhibiting antimicrobial properties has become an important area of research. Such compounds can serve as starting points for the development of new antimicrobials. In this report, we present the draft genome sequence of the Zooshikella ganghwensis strain VG4, isolated from Red Sea sediments, that produces metabolites with antimicrobial properties. A genomic analysis reveals that it carries at least five gene clusters that have the potential to direct biosynthesis of bioactive secondary metabolites such as polyketides and nonribosomal peptides...
September 2018: Biotechnology Reports
A M V N Prathyusha, G Mohana Sheela, P V Bramhachari
A filamentous fungi F. equiseti strain ANP2 with good exopolysaccharide (EPS) yield, apparent viscosity was screened and isolated from Krishna estuarine mangrove sediments. Based on molecular characteristics the selected strain was confirmed as F. equiseti, yielded EPS (4.9 ± 0.25 g/L) and whole cell biomass (3.5 ± 0.2 g/L) in MSM during early stationary phase. The crude EPS was purified by chromatography of Q-Sepharose and Superdex-75, affording Ms and MF-1 fractions were obtained, respectively...
September 2018: Biotechnology Reports
Junyoung Sim, Robertson Reid, Abid Hussain, Junyeong An, Hyung-Sool Lee
A pilot-scale dual-chamber microbial electrolysis cell (MEC) equipped with a carbon gas-diffusion cathode was evaluated for H2 O2 production using acetate medium as the electron donor. To assess the effect of cathodic pH on H2 O2 yield, the MEC was tested with an anion exchange membrane (AEM) and a cation exchange membrane (CEM), respectively. The maximum current density reached 0.94-0.96 A/m2 in the MEC at applied voltage of 0.35-1.9 V, regardless of membranes. The highest H2 O2 conversion efficiency was only 7...
September 2018: Biotechnology Reports
Manar El Samak, Samar M Solyman, Amro Hanora
Marine invertebrates-associated microorganisms were considered to be important sources of marine bioactive products. This study aims to isolate marine invertebrates associated bacteria with antimicrobial activity from the Red Sea and test their biosynthetic potential through the detection of PKS and NRPS gene clusters involved with the production of bioactive secondary metabolites. In this respect, fifty bacterial strains were isolated from eight different Red Sea marine invertebrates and screened for their antimicrobial activity against standard pathogenic bacteria ( Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633) and yeast ( Candida albicans ATCC 10231) using the standard well diffusion assay...
September 2018: Biotechnology Reports
Sudarson Sundarrajan, Sreesada Parambath, Swetha Suresh, Sneha Rao, Sriram Padmanabhan
Using Sso7d from Sulfolobus solfataricus as the DNA binding protein fused to Taq DNA polymerase at its amino terminus, we report the hyper-expression and a novel purification methodology of Sso7d-Taq polymerase (S-Taq) using aqueous two-phase extraction system followed by Ni-affinity chromatography. The utility of such a fusion enzyme in carrying out PCR of human genes from whole blood directly and in detecting hepatitis B virus from clinical samples is demonstrated in this article. We present data on the enhanced thermo-stability of S-Taq DNA polymerase over Taq DNA polymerase and also provide evidence of its higher stability with detergents in comparison to Taq polymerase...
September 2018: Biotechnology Reports
Qiang Yan, Stephen S Fong
Being capable of hydrolyzing chitin, chitinases have various applications such as production of N -acetylchitooligosaccharides (COSs) and N -acetylglucosamine (GlcNAc), degrading chitin as a consolidated bioprocessing, and bio-control of fungal phytopathogens. Here, a putative chitinase in Thermobifida fusca , Tfu_0580, is characterized. Tfu_0580 was purified by homogeneity with a molecular weight of 44.9 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Tfu_0580 displayed a clear activity against colloidal chitin, which is comparable to a commercial Streptomyces griseus chitinase...
September 2018: Biotechnology Reports
Khadijeh Babaei Sheli, Masoud Ghorbani, Azadeh Hekmat, Bita Soltanian, Alireza Mohammadian, Reza Jalalirad
Circular dichroism (CD) in far-UV region was employed to study the extent of changes occurred in the secondary structures of recombinant streptokinase (rSK), solubilized from inclusion bodies (IBs) by different chemicals and refolded/purified by chromatographic techniques. The secondary structure distribution of rSK, obtained following different chemical solubilization systems, was varied and values in the range of 12.4-14.5% α-helices, 40-51% β-sheets and 35.5-48.3% turns plus residual structures were found...
September 2018: Biotechnology Reports
Jianghui Li, Qinxia Han, Tao Zhang, Jing Du, Qianqian Sun, Yilin Pang
At present, approximately 30% of eukaryotic proteins can be expressed in a soluble form in Escherichia coli . In this study, a pCold-SUMOa plasmid was constructed in order to express heterologous proteins fused with SUMO by a cold-shock expression vector. The human cysteine desulfurase NFS1 and a chimeric cysteine desulfurase namely, EH-IscS were successfully expressed in E. coli . The proteins were particularly difficult to be produced functionally, due to their readily sequestered nature. The recombinant cysteine desulfurases that were generated by pCold-SUMOa exhibited higher activity, solubility and stability compared with the well-known plasmid pCold I...
September 2018: Biotechnology Reports
Ruijuan Hao, Zhe Zheng, Xiaodong Du, Qingheng Wang, Junhui Li, Yuewen Deng, Weiyao Chen
Pinctada fucata martensii is cultured for pearl production. Growth improvement has received considerable research interest. Transforming growth factor β type Ⅰ receptor (TβR-I), which is involved in signals transmission of transforming growth factor beta (TGF-β), participates in cell proliferation and growth. In this study, we characterized a Tgfbr1 gene which encoded TβR-I from P. fucata martensii ( Pmtgfbr1 ). Pmtgfbr1 cDNA contains an open reading frame of 1569 bp and encodes a polypeptide of 522 amino acids (aa)...
September 2018: Biotechnology Reports
Priydarshani Shinde, Mustafa Musameh, Yuan Gao, Andrea J Robinson, Ilias Louis Kyratzis
A polyvinyl alcohol (PVA) fibrous carrier has been chemically modified for the immobilization of yeast alcohol dehydrogenase (ADH) with an aim to increase its stability over a wide pH range, prolong its activity upon storage, and enhance its reusability. The strategy for immobilization involved functionalization of the fibrous carrier with chloropropinoyl chloride followed by amination with ethylenediamine. Tethering of the ADH enzyme to the PVA scaffold was achieved with glutaraldehyde. The activity profile of the immobilized enzyme was compared to soluble enzyme as a function of pH, temperature and reusability...
September 2018: Biotechnology Reports
Dary Mendoza, Olmedo Cuaspud, Juan Pablo Arias, Orlando Ruiz, Mario Arias
The objective was to enhance the production of the phenolic compounds in plant cell suspension cultures of T. peruviana at shake flask scale. The effects of salicylic acid (SA), methyl-jasmonate (MeJA) and the combination of both (SA/MeJA) were studied. Elicitor concentration, elicitation time and harvest time of cells were optimized. Phenolic compound content (PCC), flavonoid content (FC) and antioxidant activity (AA) were determined by the folin-ciocalteu method, flavonoid-aluminum complexation method and the ABTS assay, respectively...
September 2018: Biotechnology Reports
Jan Zitzmann, Christine Schreiber, Joel Eichmann, Roberto Otmar Bilz, Denise Salzig, Tobias Weidner, Peter Czermak
The generation of monoclonal cell lines is an important early process development step for recombinant protein production. Although single-cell cloning is an established method in mammalian cell lines, straightforward protocols are not yet available for insect cells. We describe a new method for the generation of monoclonal insect cells without using fetal bovine serum and/or feeder cells pretreated by irradiation or exposure to mitomycin. Highly productive clones of Drosophila melanogaster S2 cells were prepared in a two-step procedure, comprising the establishment of a polyclonal population and subsequent single cell isolation by limiting dilution...
September 2018: Biotechnology Reports
Bukola Adebayo-Tayo, Racheal Ishola, Titiloye Oyewunmi
Characterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant Weissella confusa was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type Weissella confusa (WWCEPS) had the highest EPS production. Eight (8) sugar moieties were present in the EPS. Galactose had the highest EPS composition (34.6 mg/100 g and 33.5 mg/100 g EPS) in Wild type Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS). Wild type Weissella confusa and mutant Weissella confusa EPS had antioxidant capacity...
September 2018: Biotechnology Reports
Yong Hu, Takuro Kobayashi, Guangyin Zhen, Chen Shi, Kai-Qin Xu
To investigate the influence of lipid concentration (of total solids, w/w) on anaerobic treatment of food waste under thermophilic condition, a siphon-driven self-agitated anaerobic reactor was operated for 220 days. The average lipid concentration was changed from 12.8% to 59.3% (w/w) step by step. The gas production rate increased from 1.97 to 2.31 L/L/d with lipid concentration increased from 12.8% to 19.7% (w/w), whereas decreased sharply to 0.78 L/L/d when the concentration further increased to 59...
September 2018: Biotechnology Reports
Nagalingam M, Kalpana V N, Devi Rajeswari V, Panneerselvam A
The objective of the study was to synthesize gold nanoparticles (Au NPs) using leaf extract of Alternanthera bettzickiana (A. bettzickiana ). The biosynthesized Au NPs were characterized using UV-vis spectroscopy, X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Energy dispersive X-ray analysis (EDX), Zeta potential and Transmission electron microscopy (TEM). Morphologically, the Au NPs showed spherical shaped structures. Size distribution of Au NPs calculated using Scherrer's formula, showed an average size of 80-120 nm...
September 2018: Biotechnology Reports
Linda Jansson, Ronnie Eriksson, Johannes Hedman, Moa Lavander
Molecular detection of pathogenic microorganisms in drinking and natural water is often challenged by low concentrations of the sought-after agents. Convenient methods to concentrate bacteria from water samples ranging from 1-10 L are highly warranted. Here we account for the evaluation of a lanthanum-based flocculation method to concentrate bacteria from water samples, applying four different bacterial species in tap water as well as river water. Our results show that the success of lanthanum-based flocculation is determined by both the bacterial species and the nature of the water sample...
September 2018: Biotechnology Reports
Rosa Maria Chura-Chambi, Tatiana Rodrigues Fraga, Ludmila Bezerra da Silva, Bruno Bernardi Yamamoto, Lourdes Isaac, Angela Silva Barbosa, Ligia Morganti
Enzymes from the thermolysin family are crucial factors in the pathogenesis of several diseases caused by bacteria and are potential targets for therapeutic interventions. Thermolysin encoded by the gene LIC13322 of the causative agent of leptospirosis, Leptospira interrogans , was shown to cleave proteins from the Complement System. However, the production of this recombinant protein using traditional refolding processes with high levels of denaturing reagents for thermolysin inclusion bodies (TL-IBs) solubilization results in poor recovery and low proteolytic activity probably due to improper refolding of the protein...
September 2018: Biotechnology Reports
Alexander A Osmolovskiy, Anna A Lukianova, Elena S Zvonareva, Valeriana G Kreyer, Nina A Baranova, Nikolay S Egorov
A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation...
September 2018: Biotechnology Reports
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