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Biotechnology Reports

Romana Parveen, Tooba Naz Shamsi, Gurjeet Singh, Teeba Athar, Sadaf Fatima
Triphala, an Indian ayurvedic triherbal formulation, is an equiproportional mixture of fruits of three herbs, amalaki ( Emblica officinalis ), haritaki ( Terminalia chebula ) and bibhitaki ( Terminalia bellerica ). The present study focused on phytocompounds detection and comparative analysis of various biochemical activities in the aqueous and methanolic extracts of triphala and its constituting herbs. Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), super oxide dismutase (SOD), catalase assay...
March 2018: Biotechnology Reports
Poliana M M Carvalho, Cícero A F Macêdo, Tiago F Ribeiro, Andressa A Silva, Renata E R Da Silva, Luís P de Morais, Marta R Kerntopf, Irwin R A Menezes, Roseli Barbosa
The Lippia alba (Mill.) N.E. Brown (Verbenaceae) species, has effects sedative, analgesic and spasmolytic properties. This study had as its main objective to evaluate the essential oil of L. alba (EOLa) effect and that of its main constituents, citral and limonene, over tracheal smooth muscle from Wistar rats. EOLa, citral and limonene promoted relaxation of tracheal smooth muscle in contractions induced by potassium (60 mM K+ ), presenting an EC50 of 148 ± 7 μg/mL for the EOLa, 136 ± 7 μg/mL for citral and 581 ± 7 μg/mL for limonene...
March 2018: Biotechnology Reports
Teresa Lopes da Silva, Paula C Passarinho, Ricardo Galriça, Afonso Zenóglio, Patricia Armshaw, J Tony Pembroke, Con Sheahan, Alberto Reis, Francisco Gírio
Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct production of bioethanol from microalgae through an exclusive autotrophic route. It can be concluded that the recombinant Synechocystis UL 030 containing two copies of the genes per genome was the most tolerant to ethanol...
March 2018: Biotechnology Reports
Ghanshyam Kumar Satyapal, Santosh Kumar Mishra, Amrita Srivastava, Rajesh Kumar Ranjan, Krishna Prakash, Rizwanul Haque, Nitish Kumar
In middle Gangetic plain, high arsenic concentration is present in water, which causes a significant health risk. Total 48 morphologically distinct arsenite resistant bacteria were isolated from middle Gangetic plain. The minimum inhibitory concentration (MIC) values of arsenite varied widely in the range 1-15 mM of the isolates. On the basis of their MIC, two isolates, AK1 (KY569423) and AK9 (KY569424) were selected. The analysis of the 16S rRNA gene sequence of selected isolates revealed that they are belong to the genus Pseudomonas ...
March 2018: Biotechnology Reports
Ingo de Vries, Sarah Schreiber, Daniel Boßmann, Zawadi Hellmann, Jens Kopatz, Harald Neumann, Sascha Beutel
Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked polySia was developed using a disposable bioreactor for cultivation of Escherichia coli K1 and single-use membrane adsorbers for downstream processing (DSP). The cultivation process was optimized to minimize complex media components and a maturation process after cultivation was established...
March 2018: Biotechnology Reports
Adnan B Al-Hawash, Jawadayn T Alkooranee, Hayder A Abbood, Jialong Zhang, Jin Sun, Xiaoyu Zhang, Fuying Ma
Among four crude oil-degrading fungi strains that were isolated from a petroleum-polluted area in the Rumaila oil field, two fungi strains showed high activity in aliphatic hydrocarbon degradation. ITS sequencing and analysis of morphological and biochemical characteristics identified these strains as Penicillium sp. RMA1 and RMA2. Gravimetric and gas chromatography analysis of the crude oil remaining in the culture medium after 14 days of incubation at 30 °C showed that RMA1 and RMA2 degraded the crude oil by 57% and 55%, respectively...
March 2018: Biotechnology Reports
Homa Torabizadeh, Asieh Mahmoudi
Inulinase can produce a high amount of fructose syrup from inulin in a one-step enzymatic process. Inulinase from Aspergillus niger was immobilized covalently on Fe3 O4 magnetic nanoparticles functionalized with wheat gluten hydrolysates (WGHs). Wheat gluten was enzymatically hydrolyzed by two endopeptidases Alcalase and Neutrase and related nanoparticles were prepared by desolvation method. Magnetite nanoparticles were coated with WGHs nanoparticles and then inulinase was immobilized onto it using glutaraldehyde as crosslinking agent...
March 2018: Biotechnology Reports
RaviChand Jonnadula, Md Imran, Preethi B Poduval, Sanjeev C Ghadi
Microbulbifer strain CMC-5 produces agarase, alginate lyase, xylanase, carboxymethyl cellulase and carrageenase. The extracellular production of the above carbohydrases was investigated by growing Microbulbifer strain CMC-5 in a sea water based medium containing homologous/heterologous polysaccharides as a single substrate or as a combination of mixed assorted substrate. Presence of singular homologous polysaccharides in the growth medium induces respective carbohydrase at high levels. Any two polysaccharides in various combinations produced high level of homologous carbohydrase and low level of other heterologous carbohydrase...
March 2018: Biotechnology Reports
Christian E Ogaugwu, Qiuying Cheng, Annabeth Fieck, Ivy Hurwitz, Ravi Durvasula
Constitutively active promoter elements for heterologous protein production in Lactococcus lactis are scarce. Here, the promoter of the PTS-IIC gene cluster from L. lactis NZ3900 is described. This promoter was cloned upstream of an enhanced green fluorescent protein, GFPmut3a, and transformed into L. lactis. Transformants produced up to 13.5 μg of GFPmut3a per milliliter of log phase cells. Addition of cellobiose further increased the production of GFPmut3a by up to two-fold when compared to glucose. Analysis of mutations at two specific positions in the PTS-IIC promoter showed that a 'T' to 'G' mutation within the -35 element resulted in constitutive expression in glucose, while a 'C' at nucleotide 7 in the putative cre site enhanced promoter activity in cellobiose...
March 2018: Biotechnology Reports
Alberto A Neira-Vielma, Cristóbal N Aguilar, Anna Ilyina, Juan C Contreras-Esquivel, María das Graça Carneiro-da-Cunha, Georgina Michelena-Álvarez, José L Martínez-Hernández
In this study, an extracellular phytase produced by Aspergillus niger 7A-1, was biochemically characterized for possible industrial application. The enzyme was purified from a crude extract obtained by solid-state fermentation (SSF) of triticale waste. The extract was obtained by microfiltration, ultrafiltration (300, 100 and 30 kDa) and DEAE-Sepharose column chromatography. The molecular weight of the purified enzyme was estimated to be 89 kDa by SDS-PAGE. The purified enzyme was most active at pH 5.3 and 56 °C, and retained 50% activity over a wide pH range of 4 to 7...
March 2018: Biotechnology Reports
Ramona Faas, Dirk Kiefer, Laura Job, Annelie Pohle, Karin Moß, Marius Henkel, Rudolf Hausmann
Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. The most common among these is the variant MSP1D1, which has been in the focus of research on membrane mimetics in the past. As such, the amphipathic MSP1D1 has the ability to self-assemble in the presence of synthetic phospholipids into discoidal nanoparticles, so called nanodiscs. The recombinant production of MSP is exclusively reported using a standard laboratory expression system of the pET family...
March 2018: Biotechnology Reports
Sofia Bonilla, Zahra Choolaei, Torsten Meyer, Elizabeth A Edwards, Alexander F Yakunin, D Grant Allen
Anaerobic digestion of biosludge has not yet been implemented in pulp mills due to low biogas yields. Enzymatic pretreatment of biosludge has shown improvements in biogas yields but results are varied. A key limitation of previous studies is that they fail to consider the COD contribution from the enzyme solutions. The aim of this study was to systematically investigate the potential for enzymatic pretreatment on the anaerobic digestibility of pulp mill biosludge. Out of the six enzymes tested, four enhanced the anaerobic digestibility of biosludge...
March 2018: Biotechnology Reports
Biswanath Saha, Chaichi Devi, Meena Khwairakpam, Ajay S Kalamdhad
The management of terrestrial weed is of great concern for the scientific community as these weeds cause adverse effect in different ecosystems like forest, agriculture and urban. The widespread of these weeds by their adaptive capability and morphological advancement is difficult to control. Parthenium hysterophorous , Lantana camara , Saccharum spontaneum, Ageratum conyzoides are the weeds that spread all over the world. There are various management practices employed for the control of this weeds. But all of these practices have some drawbacks those are neither environment friendly nor economical...
March 2018: Biotechnology Reports
Ramaswamy Manimekalai, Smita Nair, A Naganeeswaran, Anitha Karun, Suresh Malhotra, V Hubbali
Areca catechu L. belongs to the Arecaceae family which comprises many economically important palms. The palm is a source of alkaloids and carotenoids. The lack of ample genetic information in public databases has been a constraint for the genetic improvement of arecanut. To gain molecular insight into the palm, high throughput RNA sequencing and de novo assembly of arecanut leaf transcriptome was undertaken in the present study. A total 56,321,907 paired end reads of 101 bp length consisting of 11.343 Gb nucleotides were generated...
March 2018: Biotechnology Reports
Hala A Amin, Francesco Secundo, Hassan Amer, Faten A Mostafa, Wafaa A Helmy
Soyasapogenol B (SB) is known to have many biological activities such as hepatoprotective, anti-inflammatory, anti-mutagenic, antiviral and anticancer activities. Enzymatic conversion of soyasaponins to SB was carried out using saponin hydrolase (SH) extracted from Aspergillus flavus . The partially purified enzyme was immobilized on different carriers by physical adsorption, covalent binding or entrapment. Among the investigated carriers, Eupergit C and sugarcane bagasse (SCB) activated by DIC and NHS were the most suitable two carriers for immobilization (the immobilized forms recovered 46...
March 2018: Biotechnology Reports
Açelya Yilmazer
Cancer cells may be more prone to the accumulation of reactive oxygen species (ROS) than normal cells; therefore increased oxidative stress can specifically kill cancer cells including cancer stem cells (CSCs). In order to generate oxidative stress in various cancer cell lines including A549, G361 and MCF-7, cultured cells were exposed to H2 O2 . Incubation of cancer cells with H2 O2 results in concentration-dependent cell death in A549 and G361-7 cells, whereas MCF-7 cells showed higher sensitivity even at a lower H2 O2 concentration...
March 2018: Biotechnology Reports
Alma Toledo-Cervantes, Gloria Garduño Solórzano, Jorge E Campos, Martha Martínez-García, Marcia Morales
Scenedesmus obtusiusculus AT-UAM, isolated from Cuatro Ciénegas wetlands in Mexico was taxonomically, molecularly and biochemically compared to S. obtusiusculus CCAP 276/25 (Culture Collection of Algae and Protozoa, Scotland, UK). Analysis of Internal Transcribed Spacer 2 (ITS2) secondary structures confirmed that the mexican strain belongs to S. obtusiusculus with one change in the ITS2 nucleotide sequence. However, both strains exhibited different biochemical and fatty acid profiles and therefore biotechnological potential, emphasizing the need for deeper studies among strains of the same species...
March 2018: Biotechnology Reports
Maria Beihaghi, Hasan Marashi, Abdolreza Bagheri, Mojtaba Sankian
The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum , the construct of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agro-infiltrated into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was confirmed by quantitative Real-time PCR...
March 2018: Biotechnology Reports
Muhammad Iqbal, Saira Asif, Noshin Ilyas, Fayyaz-Ul-Hassan, Naveed Iqbal Raja, Mubashir Hussain, Muhammad Ejaz, Hafiza Saira
The experimental work presented in this study was carried out with the hypothesis that plant derived smoke enhanced the morphological, physiological and biochemical attributes of a cereal crop, wheat ( Triticum aestivum L.). Furthermore, this study supported the hypothesis that plant derived smoke acts as vegetative growth promoter, inexpensive, rapid and most appropriate eco-friendly bio-fertilizer for sustainable agriculture. Plant derived smoke was generated by burning of plant material (leaf, straws etc) in a specially designed furnace, and seeds were treated with this smoke for different time duration...
March 2018: Biotechnology Reports
Zhili Yu, Shuying Li, Yuanyuan Li, Zhuang Jiang, Jinru Zhou, Qianli An
The bacterial strain M5al is a model strain for studying the molecular genetics of N2 -fixation and molecular engineering of microbial production of platform chemicals 1,3-propanediol and 2,3-butanediol. Here, we present the complete genome sequence of the strain M5al, which belongs to a novel species closely related to Klebsiella michiganensis . M5al secretes plant cell wall-degrading enzymes and colonizes rice roots but does not cause soft rot disease. M5al also produces siderophores and contains the gene clusters for synthesis and transport of yersiniabactin which is a critical virulence factor for Klebsiella pathogens in causing human disease...
March 2018: Biotechnology Reports
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