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Biotechnology Reports

Ruijuan Hao, Zhe Zheng, Xiaodong Du, Qingheng Wang, Junhui Li, Yuewen Deng, Weiyao Chen
Pinctada fucata martensii is cultured for pearl production. Growth improvement has received considerable research interest. Transforming growth factor β type Ⅰ receptor (TβR-I), which is involved in signals transmission of transforming growth factor beta (TGF-β), participates in cell proliferation and growth. In this study, we characterized a Tgfbr1 gene which encoded TβR-I from P. fucata martensii ( Pmtgfbr1 ). Pmtgfbr1 cDNA contains an open reading frame of 1569 bp and encodes a polypeptide of 522 amino acids (aa)...
September 2018: Biotechnology Reports
Priydarshani Shinde, Mustafa Musameh, Yuan Gao, Andrea J Robinson, Ilias Louis Kyratzis
A polyvinyl alcohol (PVA) fibrous carrier has been chemically modified for the immobilization of yeast alcohol dehydrogenase (ADH) with an aim to increase its stability over a wide pH range, prolong its activity upon storage, and enhance its reusability. The strategy for immobilization involved functionalization of the fibrous carrier with chloropropinoyl chloride followed by amination with ethylenediamine. Tethering of the ADH enzyme to the PVA scaffold was achieved with glutaraldehyde. The activity profile of the immobilized enzyme was compared to soluble enzyme as a function of pH, temperature and reusability...
September 2018: Biotechnology Reports
Dary Mendoza, Olmedo Cuaspud, Juan Pablo Arias, Orlando Ruiz, Mario Arias
The objective was to enhance the production of the phenolic compounds in plant cell suspension cultures of T. peruviana at shake flask scale. The effects of salicylic acid (SA), methyl-jasmonate (MeJA) and the combination of both (SA/MeJA) were studied. Elicitor concentration, elicitation time and harvest time of cells were optimized. Phenolic compound content (PCC), flavonoid content (FC) and antioxidant activity (AA) were determined by the folin-ciocalteu method, flavonoid-aluminum complexation method and the ABTS assay, respectively...
September 2018: Biotechnology Reports
Jan Zitzmann, Christine Schreiber, Joel Eichmann, Roberto Otmar Bilz, Denise Salzig, Tobias Weidner, Peter Czermak
The generation of monoclonal cell lines is an important early process development step for recombinant protein production. Although single-cell cloning is an established method in mammalian cell lines, straightforward protocols are not yet available for insect cells. We describe a new method for the generation of monoclonal insect cells without using fetal bovine serum and/or feeder cells pretreated by irradiation or exposure to mitomycin. Highly productive clones of Drosophila melanogaster S2 cells were prepared in a two-step procedure, comprising the establishment of a polyclonal population and subsequent single cell isolation by limiting dilution...
September 2018: Biotechnology Reports
Bukola Adebayo-Tayo, Racheal Ishola, Titiloye Oyewunmi
Characterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant Weissella confusa was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type Weissella confusa (WWCEPS) had the highest EPS production. Eight (8) sugar moieties were present in the EPS. Galactose had the highest EPS composition (34.6 mg/100 g and 33.5 mg/100 g EPS) in Wild type Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS). Wild type Weissella confusa and mutant Weissella confusa EPS had antioxidant capacity...
September 2018: Biotechnology Reports
Yong Hu, Takuro Kobayashi, Guangyin Zhen, Chen Shi, Kai-Qin Xu
To investigate the influence of lipid concentration (of total solids, w/w) on anaerobic treatment of food waste under thermophilic condition, a siphon-driven self-agitated anaerobic reactor was operated for 220 days. The average lipid concentration was changed from 12.8% to 59.3% (w/w) step by step. The gas production rate increased from 1.97 to 2.31 L/L/d with lipid concentration increased from 12.8% to 19.7% (w/w), whereas decreased sharply to 0.78 L/L/d when the concentration further increased to 59...
September 2018: Biotechnology Reports
Nagalingam M, Kalpana V N, Devi Rajeswari V, Panneerselvam A
The objective of the study was to synthesize gold nanoparticles (Au NPs) using leaf extract of Alternanthera bettzickiana (A. bettzickiana ). The biosynthesized Au NPs were characterized using UV-vis spectroscopy, X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Energy dispersive X-ray analysis (EDX), Zeta potential and Transmission electron microscopy (TEM). Morphologically, the Au NPs showed spherical shaped structures. Size distribution of Au NPs calculated using Scherrer's formula, showed an average size of 80-120 nm...
September 2018: Biotechnology Reports
Linda Jansson, Ronnie Eriksson, Johannes Hedman, Moa Lavander
Molecular detection of pathogenic microorganisms in drinking and natural water is often challenged by low concentrations of the sought-after agents. Convenient methods to concentrate bacteria from water samples ranging from 1-10 L are highly warranted. Here we account for the evaluation of a lanthanum-based flocculation method to concentrate bacteria from water samples, applying four different bacterial species in tap water as well as river water. Our results show that the success of lanthanum-based flocculation is determined by both the bacterial species and the nature of the water sample...
September 2018: Biotechnology Reports
Rosa Maria Chura-Chambi, Tatiana Rodrigues Fraga, Ludmila Bezerra da Silva, Bruno Bernardi Yamamoto, Lourdes Isaac, Angela Silva Barbosa, Ligia Morganti
Enzymes from the thermolysin family are crucial factors in the pathogenesis of several diseases caused by bacteria and are potential targets for therapeutic interventions. Thermolysin encoded by the gene LIC13322 of the causative agent of leptospirosis, Leptospira interrogans , was shown to cleave proteins from the Complement System. However, the production of this recombinant protein using traditional refolding processes with high levels of denaturing reagents for thermolysin inclusion bodies (TL-IBs) solubilization results in poor recovery and low proteolytic activity probably due to improper refolding of the protein...
September 2018: Biotechnology Reports
Alexander A Osmolovskiy, Anna A Lukianova, Elena S Zvonareva, Valeriana G Kreyer, Nina A Baranova, Nikolay S Egorov
A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation...
September 2018: Biotechnology Reports
Arvind Kumar, Varun Jaiswal, Vinay Kumar, Amitava Dey, Antresh Kumar
Echinocandin B is a potent antifungal against the majority of fungal pathogens and its biosynthesis occurred by ecd and hty gene clusters in Emericella rugulosa NRRL 11440. We elucidated the functional necessity of in-clustered transcription factor; ecd B in the production of echinocandin B. We deleted the ecd B gene and found that Δ ecd B mutant has no significant effect on echinocandin B production. The expression level of most of the ecd and hty cluster genes was not significantly altered except few of them up-regulated in knockout strain...
September 2018: Biotechnology Reports
Beatriz Gil-Pulido, Emma Tarpey, Eduardo L Almeida, William Finnegan, Xinmin Zhan, Alan D W Dobson, Niall O'Leary
Dairy processing generates large volumes of wastewater that require extensive nutrient remediation prior to discharge. Significant commercial opportunities exist therefore for cost-effective biotechnologies capable of achieving this requirement. In this study the authors evaluated the use of intermittently aerated sequencing batch reactors, (IASBRs), as a single-tank biotreatment system for co-removal of COD, nitrogen and phosphorus from synthetic dairy processing wastewater. Variation of the IASBR aeration rates, (0...
September 2018: Biotechnology Reports
Geizecler Tomazetto, Sarah Hahnke, Daniel Wibberg, Alfred Pühler, Michael Klocke, Andreas Schlüter
Proteiniphilum saccharofermentans str. M3/6T is a recently described species within the family Porphyromonadaceae (phylum Bacteroidetes ), which was isolated from a mesophilic laboratory-scale biogas reactor. The genome of the strain was completely sequenced and manually annotated to reconstruct its metabolic potential regarding biomass degradation and fermentation pathways. The P. saccharofermentans str. M3/6T genome consists of a 4,414,963 bp chromosome featuring an average GC-content of 43.63%. Genome analyses revealed that the strain possesses 3396 protein-coding sequences...
June 2018: Biotechnology Reports
Anna Stuhr, Sarah Hofmann, Michael Schlömann, Michel Oelschlägel
The side-chain oxygenation of styrene is able to yield substituted phenylacetic acids from corresponding styrenes by co-metabolic transformation. This co-metabolization was investigated in Pseudomonas fluorescens ST using 4-chlorostyrene as co-substrate. It was shown that non-substituted styrene is necessary to ensure the co-metabolic process. Furthermore, aspects affecting the co-transformation were studied, e.g. cell density, amount of inducer, pH, effects of co-substrate/co-product. It was demonstrated that 4-chlorophenylacetic acid and 4-chlorostyrene are able to inhibit the reaction...
June 2018: Biotechnology Reports
Jiao Tang, Lei Shi, Lulu Li, Liangkun Long, Shaojun Ding
A 9-cis-epoxycarotenoid dioxygenase gene from Serratia sp. ATCC 39,006 ( SeNCED ) was overexpressed in soluble form in E.coli. SeNCED showed the maximum activity at 30 °C and pH 8.0, and it was stable relatively at range of pH 5-10 and temperature of 20 °C to 30 °C. SeNCED effectively catalyzes the side chain double bond cleavage of isoeugenol and 4-vinylguaiacol to vanillin. The kinetic constant Km values toward isoeugenol and 4-vinylguaiacol were 18.92 mM and 6.31 mM and V max values were 50...
June 2018: Biotechnology Reports
Jyoti Kaushal, Seema, Gursharan Singh, Shailendra Kumar Arya
The immobilization of catalase onto chitosan and chitosan-bentonite was investigated and immobilization yield of 95.91 and 95.26 was obtained respectively. The optimum pH and temperature were found as 7.5 and 8.0 at 40 °C for free and immobilized enzyme. The value of Vmax decreased by 33,000-26,300, 24,500 μmol (min mg protein)-1 and Km increased by 12.5-25 and 20 mM for free and immobilized on chitosan and chitosan-bentonite respectively. The thermal stability, half life, FTIR analyses of the beads was also performed in order to characterise the structural differences...
June 2018: Biotechnology Reports
Marwa Eltarahony, Sahar Zaki, Zeinab Kheiralla, Desouky Abd-El-Haleem
The periplasmic nitrate reductase enzyme (NAP) has become attractive catalyst, whose exploitation has emerged as one of the indispensable strategies toward environmentally benign applications. To achieve them efficiently and overcome the sensitivity of NAP in harsh environmental circumstances, the immobilization for denitrifying bacteria and NAP enzyme for simultaneous bioremediation and bionanoparticles synthesis was studied. NAP catalyzed NO3 - reduction at Vmax of 0.811 μM/min and Km of 14.02 mM. Concurrently, the immobilized MMT cells completely removed NO3 - upon 192 h with AgNPs synthesis ranging from 23...
June 2018: Biotechnology Reports
Amit Kishore Singh, Sandeep Kumar Singh, Prem Pratap Singh, Akhileshwar Kumar Srivastava, Kapil D Pandey, Ajay Kumar, Himanshu Yadav
Ulcer is one of the most common diseases affecting throughout the world population. The allopathic treatment of ulcer adversely affects the health by causing harmful side effects. Currently, many herbal plants and secondary metabolites have been used for the ulcer treatment. In the present review, many herbal plants and their parts (root, rhizome, bark, leaves and fruits) have been listed in the table are currently being used for ulcer treatment. These metabolites are responsible for ulcer-neutralization or anti-inflammatory properties...
June 2018: Biotechnology Reports
Zhiyong Lei, Alain van Mil, Junjie Xiao, Corina H G Metz, Esther C M van Eeuwijk, Pieter A Doevendans, Joost P G Sluijter
To understand and assess the roles of miRNAs, visualization of the expression patterns of specific miRNAs is needed at the cellular level in a wide variety of different tissue types. Although miRNA in situ hybridization techniques have been greatly improved in recent years, they remain difficult to routinely perform due to the complexity of the procedure. In addition, as it is crucial to define which tissues or cells are expressing a particular miRNA in order to elucidate the biological function of the miRNA, incorporation of additional stainings for different cellular markers is necessary...
June 2018: Biotechnology Reports
Heidi Jaksch-Bogensperger, Anna Hammerschmid, Ludwig Aigner, Eugen Trinka, Renate Gehwolf, Yvonne Ebner, Markus Hutterer, Sebastien Couillard-Despres
•The receptor tyrosine kinase (RTK) Axl and its ligand Gas6 are critically involved in the pathogenesis of high-grade glioma (HGG). Both proteins were found to be overexpressed e.g. in tumor cells, mediating cell proliferation and migration as well as tumor angiogenesis and neuroinflammation. The extracellular domain of Axl (sAxl) and Gas6 were found in the peri-tumoral edema and blood of animals as well as in human glioma tissue. Therefore, we monitored the level of sAxl and Gas6 in human blood samples. To increase the sensitivity of protein detection beyond commonly used standard methods we preliminary tested the innovative Proseek Single-Plex Protein Assay Kit System from Olink Bioscience together with new antibodies against the soluble RTK sAxl and its ligand Gas6...
June 2018: Biotechnology Reports
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