Ashley N Slaviero, Nipun Gorantla, Jacob Simkins, Emmanuel L Crespo, Ebenezer C Ikefuama, Maya O Tree, Mansi Prakash, Andreas Björefeldt, Lauren M Barnett, Gerard G Lambert, Diane Lipscombe, Christopher I Moore, Nathan C Shaner, Ute Hochgeschwender
SIGNIFICANCE: Luminopsins (LMOs) are bioluminescent-optogenetic tools with a luciferase fused to an opsin that allow bimodal control of neurons by providing both optogenetic and chemogenetic access. Determining which design features contribute to the efficacy of LMOs will be beneficial for further improving LMOs for use in research. AIM: We investigated the relative impact of luciferase brightness, opsin sensitivity, pairing of emission and absorption wavelength, and arrangement of moieties on the function of LMOs...
April 2024: Neurophotonics