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MicroRNA

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https://www.readbyqxmd.com/read/29984665/the-influence-of-pre-analytical-factors-on-the-analysis-of-circulating-microrna
#1
Hiromichi Shiotsu, Kazuhiro Okada, Tatsuki Shibuta, Yuki Kobayashi, Saki Shirahama, Chieri Kuroki, Saori Ueda, Masanori Ohkuma, Katsuyoshi Ikeda, Yukio Ando, Hirotaka Matsui, Yuzo Kayamori, Tsukuru Umemura
1) Background: MicroRNAs (miRNA) are expected as useful biomarkers for various diseases. We studied the pre-analytical factors causing variation in the analysis of miRNA. 2) Material and Methods: Blood samples were collected from 25 healthy subjects. Plasma and serum were obtained from the same samples. The levels of miR-451, -16, -126, and -223 were analyzed using RT-qPCR. Cel-miR-39 was added as a spiked-in control in each sample. 3) Results: With the exception of miR-451, the levels of the miRNAs in plasma were higher than in serum...
July 9, 2018: MicroRNA
https://www.readbyqxmd.com/read/29962353/functional-role-of-microrna-23b-3p-in-cancer-biology
#2
Ilaria Grossi, Alessandro Salvi, Gianluca Baiocchi, Nazario Portolani, Giuseppina De Petro
MicroRNAs (miRNAs) constitute a class of short non coding RNAs that have crucial biological roles by acting mainly as negative regulators of gene expression. The alteration of miRNAs expression has been frequently demonstrated in cancer. Furthermore, miRNAs expression data clearly revealed their possible use as diagnostic, prognostic and predictive biomarkers. In this review, we focus on the biological role of human miR-23b-3p in cancer. Several data demonstrated that miR-23b-3p targeted different genes involved in cancer aggressive properties such as proliferation, migration, invasion, and metastasis...
June 29, 2018: MicroRNA
https://www.readbyqxmd.com/read/29943706/microrna-223-regulates-septin-2-and-septin-6-in-stored-platelets
#3
Maitreyi Chattopadhyay, Neetu Dahiya, Chintamani Atreya
BACKGROUND: Septins have been identified to play important roles in platelets, but their regulation in platelets is unknown. Human platelet being an enucleated and terminally differentiated cell, mRNA downregulation by miRs is one of the posttranscriptional mechanisms operative in platelets. OBJECTIVE: Since platelets are known to have miR-223 in abundance, the objective of this study is to test whether a) platelet septins have miR-223 interacting target sites in their mRNA 3'UTRs, b) septin mRNAs and miR-223 form complexes with Argonaute 2 (AGO2) protein in platelets, which is the catalytic component of an RNA Induced Silencing Complex (RISC), c) a reporter gene with septin mRNA 3' untranslated region (UTR) is subjected to downregulation by miR-223 and d) anti-miR-223 can suppress miR-223 activity and enhance septin-2 expression in platelets...
June 26, 2018: MicroRNA
https://www.readbyqxmd.com/read/29848284/serum-mir-29a-and-mir-122-as-potential-biomarkers-for-non-alcoholic-fatty-liver-disease-nafld
#4
Kanisa Jampoka, Puth Muangpaisarn, Kritsada Khongnomnan, Sombat Treeprasertsuk, Pisit Tangkijvanich, Sunchai Payungporn
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is an over accumulation of triglyceride in the liver without alcohol consumption which its major cause is from insulin resistance. Patients with NAFLD can develop to be liver fibrosis, cirrhosis and hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) are non-coding RNAs that regulate post-transcriptional gene silencing. Previous research reported that miR-29 family (a, b and c) and miR-122 have an important role in regulating insulin resistance related to NAFLD...
May 30, 2018: MicroRNA
https://www.readbyqxmd.com/read/29793420/update-on-the-regulation-of-hipk1-hipk2-and-hipk3-protein-kinases-by-micrornas
#5
Andrea Conte, Giovanna Maria Pierantoni
The homeodomain-interacting protein kinases (HIPKs) HIPK1, HIPK2 and HIPK3 are Ser/Thr kinases which interact with homeobox proteins and other transcription factors, acting as transcriptional coactivators or corepressors. HIPKs contribute to regulating several biological processes, such as signal transduction, apoptosis, embryonic development, DNA-damage response, and cellular proliferation, in response to various extracellular stimuli. Recently it has emerged that, in addition to their role in cancer, fibrosis and diabetes, HIPKs may also be involved in other human diseases, including amyotrophic lateral sclerosis (ALS), Rett syndrome, cerebellar diseases, and retinal vascular dysfunction...
May 24, 2018: MicroRNA
https://www.readbyqxmd.com/read/29793419/role-of-microrna-in-the-diagnosis-and-therapy-of-hepatic-metastases-from-colorectal-cancer
#6
Angeliki Chorti, Petros Bangeas, Theodossis S Papavramidis, Georgios Tsoulfas
Colorectal cancer is one of the most common malignancies in both genders and liver metastasis appear in more than 50% of patients with colorectal cancer, worsening its morbidity and mortality rates. The existing methods for the diagnosis and prognosis of colorectal cancer seem to be insufficient to predict its aggressiveness, leading to poor outcomes for the patient. MicroRNAs are small non-coding RNAs, which interact with mRNAs in a post-transcriptional stage, and have been found to be involved in pathogenesis of cancer and its metastases...
May 24, 2018: MicroRNA
https://www.readbyqxmd.com/read/29779489/rap1-downregulation-by-mir-320c-reduces-platelet-activation-in-ex-vivo-storage
#7
Chintamani Atreya, Neetu Dahiya
BACKGROUND: A small GTPase protein, the Ras-related protein 1 (RAP1), abundant in platelets is known to be activated following agonist induced platelet activation, suggesting that RAP1 downregulation could in turn reduce platelet activation in storage. Our objective of this study is to identify RAP1 regulating miRNAs and their role in platelet activation during storage. METHODS: We applied MS2-TRAP (tagged RNA affinity purification) methodology to enrich miRNAs that target the 3' untranslated region (3'UTR) of RAP1 mRNA in two mammalian cell lines followed by miRNA identification by microarray of total RNA samples enriched for miRNAs...
May 20, 2018: MicroRNA
https://www.readbyqxmd.com/read/29766833/human-micrornas-expression-profiles-in-influenza-b-virus-infected-cells-based-on-illumina-miseq-platform
#8
Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Suthat Sangchuwong, Trairak Pisitkun, Yong Poovorawan, Sunchai Payungporn
BACKGROUND: Influenza B virus causes influenza-like illness in humans. MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression through mRNA degradation or translational repression. MiRNAs have evolved to regulate many cellular processes including the viral infection response. OBJECTIVE: This study aims to investigate the miRNA profiles of human cells infected with influenza B virus. METHODS: A549 cells were infected with influenza B viruses (MOI = 0...
May 14, 2018: MicroRNA
https://www.readbyqxmd.com/read/29714155/investigating-the-isolation-and-amplification-of-micrornas-for-forensic-body-fluid-identification
#9
Claire L Glynn, Kelsie R O Leary
BACKGROUND: The discovery of forensic DNA typing evolved molecular biology far beyond what could have been expected in terms of its forensic application, and now there exists other developments in molecular biology which are ready for application to forensic challenges. One such challenge is the identification of the body fluid source of stains recovered from evidence items and crime scenes. Currently there are significant efforts in the research field to develop novel methods for the molecular identification of body fluids, with microRNAs (miRNAs) revealing great potential...
April 30, 2018: MicroRNA
https://www.readbyqxmd.com/read/29701140/expression-of-plasma-hsa-mir122-in-hbv-related-hepatocellular-carcinoma-hcc-in-vietnamese-patients
#10
Nguyen Bao Quoc, Nguyen Doan Nguyen Phuong, Tang Kim Ngan, Nguyen Thi Minh Linh, Pham Hung Cuong, Nguyen Ngoc Bao Chau
BACKGROUND: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related death in the world and considered as one of the most susceptible cancers in humans. The microRNA molecule, hsa-miR122, considered as a potential biological marker linked with the injury of hepatocellular tissue, is the most common microRNA in human liver cancer. Understanding the expression profile of hsa-miR122 plays an important role in the diagnosis of HCC Objective: Identification and comparison of cut-off values of plasma hsa-miR122 expression were conducted in blood samples of healthy control, HBV infected and HBV-related HCC Vietnamese patients Method and result: Fifty-two blood samples of healthy control and HBV-related HCC cases, collected between 2015 and 2017 were obtained from Ho Chi Minh City Oncology Hospital, Vietnam...
April 27, 2018: MicroRNA
https://www.readbyqxmd.com/read/29658445/improved-detection-of-circulating-mirnas-in-serum-and-plasma-following-rapid-heat-freeze-cycling
#11
Peter D Mariner, Armin Korst, Anis Karimpour-Fard, Brian L Stauffer, Shelley D Miyamoto, Carmen C Sucharov
BACKGROUND: The measurement of circulating miRNAs has proven to be a powerful biomarker tool for several disease processes. Current protocols for the detection of miRNAs usually involve an RNA extraction step, requiring a substantial volume of patient serum or plasma to obtain sufficient input material. OBJECTIVE: Here, we describe a novel methodology that allows detection of a large number of miRNAs from a small volume of serum or plasma without the need for RNA extraction...
April 16, 2018: MicroRNA
https://www.readbyqxmd.com/read/29607782/influence-of-overweight-and-obesity-on-circulating-inflammation-related-microrna
#12
Jamie G Hijmans, Kyle J Diehl, Tyler D Bammert, Philip J Kavlich, Grace M Lincenberg, Jared J Greiner, Brian L Stauffer, Christopher A DeSouza
BACKGROUND: Increased cardiovascular disease risk and prevalence associated with overweight and obesity is due, in part, to heightened inflammatory burden. The mechanisms underlying adiposity-related amplification of inflammation are not fully understood. Alterations in regulators of inflammatory processes such as microRNAs (miRs), however, is thought to play a pivotal role. OBJECTIVE: The aim of this study was to determine the influence of overweight and obesity, independent of other cardiovascular risk factors, on circulating expression of miR-34a, miR-126, miR-146a, miR-150 and miR-181b...
April 2, 2018: MicroRNA
https://www.readbyqxmd.com/read/29595121/the-expansion-segments-of-human-28s-rrna-match-micrornas-much-above-18s-rrna-or-core-segments
#13
Michael S Parker, Ambikaipakan Balasubramaniam, Steven L Parker
The size of eukaryotic 25-28S rRNAs shows a progressive phylogenetically linked increase which is pronounced in mammals, and especially in hominids. The increase is confined to specific expansion segments, inserted at points that are highly conserved from yeast to man. These segments also show a progressive increase in nucleotide bias, mostly the GC bias. Substantial parts of the large expansion segments 7, 15 and 27 of 28S rRNA are known to be exposed at the ribosome surface, with no clear association with ribosomal proteins...
March 28, 2018: MicroRNA
https://www.readbyqxmd.com/read/29595120/state-of-the-art-on-viral-micrornas-in-hpv-infection-and-cancer-development
#14
Palmiro Poltronieri, Binlian Sun, Kai-Yao Huang, Tzu-Hao Chang, Tzong-Yi Lee
BACKGROUND: high-risk HPV subtypes are driving forces for human cancer development: HPV-16 and HPV-18 are responsible for most HPV-caused cancers. OBJECTIVE: This review describes the present knowledge on HR-HPV genomes coding potential for viral miRNAs. METHOD: HPV subtypes miRNA database, VIRmiRtar, has been constructed applying bioinformatics and a computational method, ViralMir, exploiting structural features, presence of hairpins, and validation by comparison with RNA sequencing datasets...
March 27, 2018: MicroRNA
https://www.readbyqxmd.com/read/29564990/impact-of-delayed-whole-blood-processing-time-on-plasma-levels-of-mir-1-and-mir-423-5p-up-to-24-hours
#15
Danielle Pazzotti Borges, Edecio Cunha-Neto, Edimar A Bocchi, Vagner Oliveira Carvalho Rigaud
BACKGROUND: Circulating cell-free miRNAs hold great promise as a new class of biomarkers due to their high stability in body fluids and association with disease stages. However, even using sensitive and specific methods, technical challenges are associated with miRNA analysis in body fluids. A major source of variation in plasma and serum is the potential cell-derived miRNA contamination from hemolysis. OBJECTIVES: To evaluate the effect of the delayed whole blood processing time on the concentrations of miR-1 and -423-5p...
March 21, 2018: MicroRNA
https://www.readbyqxmd.com/read/29437031/underexpression-of-mir-486-5p-but-not-overexpression-of-mir-155-is-associated-with-lung-cancer-stages
#16
Marwa A Mohamed, Ehab I Mohamed, Samir A Abd El-Kaream, Mohamed I Badawi, Samy H Darwish
BACKGROUND: Evidence is increasing that microRNAs (miR) are particularly important in lung homeostasis and development and have been shown to be involved in many pulmonary diseases such as asthma, chronic obstructive pulmonary disease, sarcoidosis, lung cancer (LC) and other smoking-related diseases. OBJECTIVE: The objective of this study was to investigate the expression of miR-155 and miR-486-5p in tissues from LC patients and healthy endobronchial mucosa as prognostic biomarkers for diagnosing LC...
February 12, 2018: MicroRNA
https://www.readbyqxmd.com/read/29412128/variation-in-immune-related-micrornas-profile-in-human-milk-amongst-lactating-women
#17
Mariarita Perri, Maria Lucente, Roberto Cannataro, Ida Francesca De Luca, Luca Gallelli, Guido Moro, Giovambattista De Sarro, Maria Cristina Caroleo, Erika Cione
BACKGROUND: Human milk (HM) is a biological fluid representing the first nutriment for newborns. It directly impacts the development of the infant's immune system. In this concern, specific microRNAs (miRNAs) such as hsa-miR-21, hsa-miR-181a, hsa-miR-150 and hsa-miR-223 are known to be involved in the innate and acquired immune response. OBJECTIVE: Herein, these miRNAs were evaluated in frozen and pasteurized samples of human colostrum and HM in order to elucidate the distribution and the expression profile of these biological mediators in both biological fluids...
February 6, 2018: MicroRNA
https://www.readbyqxmd.com/read/29412127/investigating-the-association-between-mir-608-rs4919510-and-mir-149-rs2292832-with-colorectal-cancer-in-iranian-population
#18
Reza Ranjbar, Vahid Chaleshi, Hamid Asadzadeh Aghdaei, Saeid Morovvati
BACKGROUND: Single nucleotide polymorphisms (SNPs) in microRNA (miRNA) networks may serve as diagnostic and prognostic biomarkers of a variety of diseases such as cancer. Some studies have been performed to examine associations between miR-149 and miR-608 polymorphisms and susceptibility to colorectal cancer, but the results remain controversial and race-dependent. OBJECTIVE: The aim of our study was to investigate the association of miR-608 (rs4919510) and miR-149 (rs2292832) with colorectal cancer and its clinical features in a sample of Iranian population...
February 6, 2018: MicroRNA
https://www.readbyqxmd.com/read/29380708/technology-of-rna-interference-in-advanced-medicine
#19
Fatemeh Abedini, Mohammad Ebrahimi, Hossein Hosseinkhani
RNA interference (RNAi) and related pathways involving small interfering RNAs (siRNAs), microRNAs (miRNAs), and PIWI-interacting RNAs (piRNAs) regulate processes such as anti-viral defense, genome surveillance, heterochromatin formation, and gene expression in animals, plants, and fungi. Studies on RNAi have revealed a two-step mechanism: (i) Degradation of dsRNA into small interfering RNAs (siRNAs), 21 to 25 nucleotides long, by an RNase III-like activity. (ii) The siRNAs join an RNase complex, RISC (RNA-induced silencing complex), which acts on the mRNA and degrades it...
January 29, 2018: MicroRNA
https://www.readbyqxmd.com/read/29648524/preface-the-wealth-of-microrna-kingdom-from-plant-defense-to-cancer-prevention
#20
Alberto Izzotti
No abstract text is available yet for this article.
2018: MicroRNA
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