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World Journal of Methodology

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https://www.readbyqxmd.com/read/27679782/adjuvant-role-of-pseudomonas-flagellin-for-acinetobacter-baumannii-biofilm-associated-protein
#1
Mozhgan Derakhshan Sefidi, Iraj Rasooli, Parviz Owlia, Daryush Talei, Shakiba Darvish Alipour Astaneh, Shahram Nazarian
AIM: To study immunogenicity of Pseudomonas N terminal flagellin as an adjuvant for Acinetobacter baumannii (A. baumannii) biofilm associated protein (Bap). METHODS: The N terminal flagellin gene was amplified. The pET28a (+) and polymerase chain reaction products were digested with HindIII and EcoR I. The ligation of N terminal flagellin into pET28a (‏+) was performed using T4 DNA ligase and was then transformed into Escherichia coli BL21 (DE3) as a suitable expression host...
September 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27679781/modified-skin-bridge-technique-for-ilio-inguinal-lymph-node-dissection-a-forgotten-technique-revisited
#2
REVIEW
Mukur Dipi Ray, Pankaj K Garg, Ashish Jakhetiya, Sunil Kumar, Durgatosh Pandey
Ilio-inguinal lymph node dissection (IILD) is a commonly performed surgical procedure for a number of malignant conditions involving mainly the male and female genitalia, and the skin; however the postoperative morbidity of IILD, due to high frequency of flap necrosis, wound infection and seroma formation, has always been a major concern for the surgeons. The aim of the study is to highlight a modified skin bridge technique of IILD using two parallel curvilinear incisions to minimize postoperative skin flap necrosis...
September 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27679780/cd4-positive-diffuse-large-b-cell-lymphoma-a-variant-with-aggressive-clinical-potential
#3
REVIEW
Mohammad O Hussaini, Friederike H Kreisel, Anjum Hassan, TuDung T Nguyen, John L Frater
CD4 expression is rare in diffuse large B-cell lymphoma (DLBCL), with 4 previously reported cases. Its significance is uncertain. We report five patients with CD4(+) DLBCL and one CD4(+) primary mediastinal large B-cell lymphoma. Cases were identified by searching the electronic database of the department; each was reviewed. Average age was 56 years. Neoplastic cells expressed CD20 (5/6 tested cases). BCL2/BCL6 expression were seen in 3/3 tested cases, suggesting a germinal center origin. Additionally, expression of T-cell antigens CD2 and CD5 was noted in 2/2 and CD7 in 1/1 tested case...
September 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27376022/footwear-modification-following-hallux-valgus-surgery-the-all-or-none-phenomenon
#4
Cal Robinson, Abhijit Bhosale, Anand Pillai
AIM: To define footwear outcomes following hallux valgus surgery, focusing on patient return to comfortable and heeled footwear and patterns of post-operative footwear selection. METHODS: Surgical intervention is indicated for symptomatic cases of hallux valgus unresponsive to conservative methods, with favourable reported outcomes. The return to various types of footwear post-operatively is reflective of the degree of correction achieved, and corresponds to patient satisfaction...
June 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27376021/mild-oxidative-stress-is-beneficial-for-sperm-telomere-length-maintenance
#5
Swetasmita Mishra, Rajeev Kumar, Neena Malhotra, Neeta Singh, Rima Dada
AIM: To evaluate telomere length in sperm DNA and its correlation with oxidative stress (normal, mild, severe). METHODS: The study included infertile men (n = 112) and age matched fertile controls (n = 102). The average telomere length from the sperm DNA was measured using a quantitative real time PCR based assay. Seminal reactive oxygen species (ROS) and 8-Isoprostane (8-IP) levels were measured by chemiluminescence assay and ELISA respectively. RESULTS: Average sperm telomere length in infertile men and controls was 0...
June 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27376020/rat-model-of-cholelithiasis-with-human-gallstones-implanted-in-cholestasis-induced-virtual-gallbladder
#6
Marlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Yue Li, Jian Zhang, Raymond Oyen, Yicheng Ni
AIM: To facilitate translational research on cholelithiasis, we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this species. METHODS: Under anesthesia, 16 adult rats of equal genders underwent two times of abdominal surgery. First, their common bile duct (CBD) was ligated to cause cholestasis by total biliary obstruction (TBO). On day 0, 1, 3, 7, 14, 21 and 28 after TBO, magnetic resonance imaging (MRI) was conducted to monitor the dilatation of the CBD, and blood was sampled to analyze total serum bilirubin (TSB)...
June 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27376019/methodology-in-improving-antibiotic-implementation-policies
#7
REVIEW
Onur Özgenç
The basic requirements of antibiotic prescribing are components of methodology; knowledge, logical reasoning, and analysis. Antimicrobial drugs are valuable but limited resources, different from other drugs and they are among the most commonly prescribed drugs all over the world. They are the only drugs which do not intentionally affect the patient. They affect the pathogens which invade the host. The emergence and spread of antibiotic-resistant pathogens are accelerated by heavy antibiotic usage. The effective antimicrobial stewardship and infection control program have been shown to limit the emergence of antimicrobial-resistant bacteria...
June 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27376018/methods-units-and-quality-requirements-for-the-analysis-of-haemoglobin-a1c-in-diabetes-mellitus
#8
REVIEW
Ilkka Penttilä, Karri Penttilä, Päivi Holm, Harri Laitinen, Päivi Ranta, Jukka Törrönen, Rainer Rauramaa
The formation of glycohemoglobin, especially the hemoglobin A1c (HbA1c) fraction, occurs when glucose becomes coupled with the amino acid valine in the β-chain of Hb; this reaction is dependent on the plasma concentration of glucose. Since the early 1970s it has been known that diabetics display higher values OF HbA1C because they have elevated blood glucose concentrations. Thus HbA1c has acquired a very important role in the treatment and diagnosis of diabetes mellitus. After the introduction of the first quantitative measurement OF HbA1C, numerous methods for glycohemoglobin have been introduced with different assay principles: From a simple mini-column technique to the very accurate automated high-pressure chromatography and lastly to many automated immunochemical or enzymatic assays...
June 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019804/efficient-management-and-maintenance-of-ultrasonic-nebulizers-to-prevent-microbial-contamination
#9
Yoko Ida, Hiroaki Ohnishi, Kouji Araki, Ryoichi Saito, Shin Kawai, Takashi Watanabe
AIM: To seek the cause of Burkholderia cepacia complex (Bcc) infection outbreak and evaluate the efficacy of new methods for nebulizer maintenance. METHODS: We investigated the annual number of Bcc isolates recovered from clinical samples in our hospital between 1999 and 2013. Swab samples were randomly collected for bacterial culture before patient use from 10 each of the two machine types in August 2001; these included 20 samples from each of the following: Drain tubes, operating water chambers, oscillators, and nebulizing chambers...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019803/profile-and-determinants-of-unsuccessful-tuberculosis-outcome-in-rural-nigeria-implications-for-tuberculosis-control
#10
Kingsley N Ukwaja, Sarah N Oshi, Isaac Alobu, Daniel C Oshi
AIM: To determine the treatment outcomes and predictors for unsuccessful tuberculosis (TB) outcomes in rural Nigeria. METHODS: Adult rural TB patients treated during 2011 and 2012 in two healthcare facilities (one urban public and one rural private) were identified from the TB treatment registers and retrospectively reviewed. Tuberculosis treatment outcomes were assessed according to World Health Organisation guidelines. Determinants of unsuccessful treatment outcomes were identified using a multivariable logistic regression analysis...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019802/potential-effects-of-curcumin-on-peroxisome-proliferator-activated-receptor-%C3%AE-in-vitro-and-in-vivo
#11
REVIEW
Mohsen Mazidi, Ehsan Karimi, Mohsen Meydani, Majid Ghayour-Mobarhan, Gordon A Ferns
Natural peroxisome proliferator-activated receptor-γ (PPAR-γ) agonists are found in food and may be important for health through their anti-inflammatory properties. Curcumin (Cur) is a bright yellow spice, derived from the rhizome of Curcuma longa Linn. It has been shown to have many biological properties that appear to operate through diverse mechanisms. Some of these potentially beneficial effects of Cur are due to activation of the nuclear transcription factor PPAR-γ. It is reported (using in vitro and in vivo models) that Cur plays a potential role against several diseases...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019801/study-design-in-evidence-based-surgery-what-is-the-role-of-case-control-studies
#12
REVIEW
Amy M Cao, Michael R Cox, Guy D Eslick
Randomized controlled trials (RCTs) are the gold standard in terms of study design, however, in the surgical setting conducting RCTs can often be unethical or logistically impossible. Case-control studies should become the major study design used in surgical research when RCTs are unable to be conducted and definitely replacing case series which offer little insight into surgical outcomes and disease processes.
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019800/standardization-in-laboratory-medicine-adoption-of-common-reference-intervals-to-the-croatian-population
#13
REVIEW
Zlata Flegar-Meštrić, Sonja Perkov, Andrea Radeljak
Considering the fact that the results of laboratory tests provide useful information about the state of health of patients, determination of reference value is considered an intrinsic part in the development of laboratory medicine. There are still huge differences in the analytical methods used as well as in the associated reference intervals which could consequently significantly affect the proper assessment of patient health. In a constant effort to increase the quality of patients' care, there are numerous international initiatives for standardization and/or harmonization of laboratory diagnostics in order to achieve maximum comparability of laboratory test results and improve patient safety...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019799/cutaneous-perivascular-epithelioid-cell-tumors-a-review-on-an-infrequent-neoplasm
#14
REVIEW
Mar Llamas-Velasco, Luis Requena, Thomas Mentzel
"Perivascular epithelioid cutaneous" cell tumors (PEComa) are a family of mesenchymal tumors with shared microscopic and immunohistochemical properties: They exhibit both smooth muscle cell and melanocytic differentiation. Non-neoplastic counterpart of PEComa's cells are unknown, as well as the relationship between extracutaneous PEComa and primary cutaneous ones. We will review the clinical setting, histopathologic features, chromosomal abnormalities, differential diagnosis and treatment options for cutaneous PEComa...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019798/vascular-targeted-photochemotherapy-using-padoporfin-and-padeliporfin-as-a-method-of-the-focal-treatment-of-localised-prostate-cancer-clinician-s-insight
#15
REVIEW
Andrzej M Bugaj
Vascular targeted photochemotherapy (VTP) holds promise as a novel strategy of the focal treatment of localised prostate cancer (LPCa). It is convenient to perform, minimally invasive and can be conduct in ambulatory conditions. In this review, methodologic aspects of padoporfin- and padeliporfin-mediated VTP and its clinical application in focal treatment of LPCa as well as future perspective of this method were presented. Physicochemical and pharmacokinetic parameters of padoporphin and padeliporfin using as VTP photosensitizers were described, as well as methodologic question of radiation delivery and dosimetry, and oxygen monitoring in cancer tissue in context of VTP safety and efficiency of LPCa focal therapy were discussed...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019797/quo-vadis-motor-neuron-disease
#16
REVIEW
Rubika Balendra, Rickie Patani
Motor neuron disease (MND), also known as amyotrophic lateral sclerosis, is a relentlessly progressive neurodegenerative condition that is invariably fatal, usually within 3 to 5 years of diagnosis. The aetio-pathogenesis of MND remains unresolved and no effective treatments exist. The only Food and Drug Administration approved disease modifying therapy is riluzole, a glutamate antagonist, which prolongs survival by up to 3 mo. Current management is largely symptomatic/supportive. There is therefore a desperate and unmet clinical need for discovery of disease mechanisms to guide novel therapeutic strategy...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019796/oncogenic-role-of-leptin-and-notch-interleukin-1-leptin-crosstalk-outcome-in-cancer
#17
REVIEW
Crystal C Lipsey, Adriana Harbuzariu, Danielle Daley-Brown, Ruben R Gonzalez-Perez
Obesity is a global pandemic characterized by high levels of body fat (adiposity) and derived-cytokines (i.e., leptin). Research shows that adiposity and leptin provide insight on the link between obesity and cancer progression. Leptin's main function is to regulate energy balance. However, obese individuals routinely develop leptin resistance, which is the consequence of the breakdown in the signaling mechanism controlling satiety resulting in the accumulation of leptin. Therefore, leptin levels are often chronically elevated in human obesity...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019795/clinical-development-of-reovirus-for-cancer-therapy-an-oncolytic-virus-with-immune-mediated-antitumor-activity
#18
REVIEW
Jun Gong, Esha Sachdev, Alain C Mita, Monica M Mita
Reovirus is a double-stranded RNA virus with demonstrated oncolysis or preferential replication in cancer cells. The oncolytic properties of reovirus appear to be dependent, in part, on activated Ras signaling. In addition, Ras-transformation promotes reovirus oncolysis by affecting several steps of the viral life cycle. Reovirus-mediated immune responses can present barriers to tumor targeting, serve protective functions against reovirus systemic toxicity, and contribute to therapeutic efficacy through antitumor immune-mediated effects via innate and adaptive responses...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019794/cation-exchange-high-performance-liquid-chromatography-for-variant-hemoglobins-and-hbf-a2-what-must-hematopathologists-know-about-methodology
#19
Prashant Sharma, Reena Das
Cation-exchange high-performance liquid chromatography (CE-HPLC) is a widely used laboratory test to detect variant hemoglobins as well as quantify hemoglobins F and A2 for the diagnosis of thalassemia syndromes. It's versatility, speed, reproducibility and convenience have made CE-HPLC the method of choice to initially screen for hemoglobin disorders. Despite its popularity, several methodological aspects of the technology remain obscure to pathologists and this may have consequences in specific situations...
March 26, 2016: World Journal of Methodology
https://www.readbyqxmd.com/read/27019793/new-developments-and-controversies-in-iron-metabolism-and-iron-chelation-therapy
#20
Christina N Kontoghiorghe, George J Kontoghiorghes
Iron is essential for all organisms including microbial, cancer and human cells. More than a quarter of the human population is affected by abnormalities of iron metabolism, mainly from iron deficiency and iron overload. Iron also plays an important role in free radical pathology and oxidative damage which is observed in almost all major diseases, cancer and ageing. New developments include the complete treatment of iron overload and reduction of morbidity and mortality in thalassaemia using deferiprone and selected deferiprone/deferoxamine combinations and also the use of the maltol iron complex in the treatment of iron deficiency anaemia...
March 26, 2016: World Journal of Methodology
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