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Journal of Global Antimicrobial Resistance

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https://www.readbyqxmd.com/read/30217548/first-report-of-blaoxa-24-carbapenemase-encoding-gene-arma-methyltransferase-and-aac-6-ib-cr-producing-multidrug-resistant-clinical-isolates-of-proteus-mirabilis-in-algeria
#1
Zineb Leulmi, Chouaib Kandouli, Ilhem Mihoubi, Kaddour Benlabed, Abdeslam Lezzar, Jean-Marc Rolain
BACKGROUND: Carbapenemase-producing, or carbapenem-resistant Enterobacteriaceae, are an emerging threat to human and animal health because they are resistant to many of the last-line antimicrobials available for disease treatment. The aim of this study was to analyze antimicrobial resistance patterns and their encoding genes of P. mirabilis isolated in Constantine, Algeria. METHODS: A total of 106 PMP (Proteus- Morganella- Providencia) strains were isolated from a large variety of clinical specimens, at University Hospital of Constantine in Algeria, and identified by the API 20E system and Bruker MALDI Biotyper 2...
September 11, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30213719/genomic-analysis-of-the-first-isolate-of-klebsiella-pneumoniae-bla-kpc-2-producing-from-uruguay
#2
Verónica E Álvarez, Josefina Campos, Antonio Galiana, Graciela Borthagaray, Daniela Centrón, Carolina Márquez Villalba
OBJECTIVES: Since blaKPC-2 -producing Klebsiella pneumoniae (KPC-Kp) are related to the successful dissemination of a major clone defined as sequence type 258 (ST258), our goal was to perform whole-genome sequencing (WGS) of the first colistin-resistant K. pneumoniae strain (Kpn666) carrying blaKPC-2 identified in Uruguay in 2011 in order to identify genomic and phylogenetic traits. METHODS: WGS of Kpn666 strain isolated from an asymptomatic urinary infection was performed using Illumina MiSeq and de novo assembly was done using SPADES version 3...
September 10, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30213718/antimicrobial-resistance-virulence-gene-profiles-and-molecular-typing-of-staphylococcus-aureus-isolates-from-dairy-cows-in-xinjiang-northwest-of-china
#3
Meng Dan, Wu Yehui, Meng Qingling, Qiao Jun, Zhang Xingxing, Ma Shuai, Cai Kuojun, Zhang Jinsheng, Cheng Zibing, Zhang Zaichao, Cai Xuepeng
BACKGROUND: Staphylococcus aureus (SA) is one of the major pathogens causing dairy cow mastitis and endometritis. Recently, animal-derived SA strains have posed a serious threat to public health. However, little is known about antimicrobial resistance and virulence factors of SA strains isolated from dairy cows in Xinjiang China. Here, we characterized antimicrobial resistance, virulence gene profiles and genotypes of SA isolates from clinical mastitis and endometritis in dairy cows. METHODS: A total of 337 clinical samples (including 186 milk samples from cases of clinical mastitis and 151 swab samples of endometritis) were collected from 15 large-scale dairy farms in Xinjiang China and screened for SA...
September 10, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29908916/detection-and-molecular-characterisation-of-extended-spectrum-%C3%AE-lactamase-producing-enteric-bacteria-from-pigs-and-chickens-in-nsukka-nigeria
#4
Kennedy F Chah, Ifeoma C Ugwu, Angela Okpala, Kwabugge Y Adamu, Carla Andrea Alonso, Sara Ceballos, John N Nwanta, Carmen Torres
OBJECTIVES: This study screened chickens and pigs slaughtered for human consumption for the presence and characteristics of extended-spectrum β-lactamase (ESBL)- and plasmid-encoded AmpC (pAmpC) β-lactamase-producing enteric bacteria. METHODS: Faecal samples from 410 broiler chickens and 100 pigs were cultured on MacConkey agar supplemented with 2μg/mL cefotaxime. Antimicrobial resistance phenotypes of the recovered isolates were determined by disk diffusion...
September 5, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30172833/draft-genome-sequence-of-kpc-2-producing-pseudomonas-aeruginosa-recovered-from-a-bloodstream-infection-sample-in-brazil
#5
Ivson Cassiano de Oliveira Santos, Rodolpho Mattos Albano, Marise Dutra Asensi, Ana Paula D'Alincourt Carvalho-Assef
OBJECTIVES: KPC is the most widespread carbapenemase in Enterobacteriaceae in Brazil. Despite its presence is not common in Pseudomonas aeruginosa it has been increasingly reported. Here we report a draft genome sequence of a KPC-producing P. aeruginosa recovered from a bloodstream infection sample in Brazil. METHODS: The antimicrobial susceptibility of KPC-producing P. aeruginosa CCBH17348 isolate was evaluated by disc diSffusion, E-test and broth microdilution...
August 30, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30172832/emergence-of-ctx-m-1-producing-salmonella-enterica-serovar-napoli-a-novel-enzyme-pathogen-association-in-the-italian-esbl-endemic-context
#6
LETTER
Luigi Principe, Valentina Viaggi, Mathieu Clément, Elisa Meroni, Beatrice Pini, Andrea Endimiani, Francesco Luzzaro
No abstract text is available yet for this article.
August 30, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30172831/genetic-characterization-of-methicillin-resistant-staphylococcus-aureus-and-staphylococcus-pseudintermedius-in-pets-and-veterinary-personnel-s-in-iran-new-insights-into-emerging-mrsp
#7
Saeid Tabatabaei, Arash Najafifar, Mahdi Askari Badouei, Taghi Zahraei Salehi, Iradj Ashrafi Tamai, Ehsan Khaksar, Mohamed Salah Abbassi, Fereshteh Ghazisaeedi
INTRODUCTION: Methicillin resistant staphylocci including Staphylococcus aureus (MRSA) and S. pseudintermedius (MRSP) pose threats to animal and human health worldwide. Veterinary staffs and pets may play a role in spreading of resistant clones. METHODS: A total number of 125 samples from veterinary staff (n=50), dogs (n=49) and cats (n=26) were investigated. The obtained isolates were tested for the presence of mecA gene conferring resistance to methicillin, and then the isolates were subjected to a multiplex-PCR differentiating the coagulase-positive species...
August 30, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30172045/use-of-whole-genome-sequencing-to-predict-mycobacterium-tuberculosis-drug-resistance-in-indonesia
#8
Lidya Chaidir, Carolien Ruesen, Bas E Dutilh, Ahmad R Ganiem, Anggriani Andryani, Lika Apriani, Martijn A Huynen, Rovina Ruslami, Philip C Hill, Reinout van Crevel, Bachti Alisjahbana
BACKGROUND: Whole genome sequencing (WGS) is rarely used for drug-resistance testing of Mycobacterium tuberculosis in high-endemic settings. We present the first study from Indonesia, which has the second highest tuberculosis (TB) burden worldwide, with less than 50% of drug-resistant cases currently detected. METHODS: We applied WGS in strains from 322 adult HIV-negative TB patients. Phenotypic DST was done for a portion of patients. RESULTS: Fifty-one isolates (15...
August 29, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30172044/isaba36-inserted-into-the-outer-membrane-protein-gene-caro-and-associated-with-the-carbapenemase-gene-bla-oxa-24-like-in-acinetobacter-baumannii
#9
LETTER
Khadidja Khorsi, Yamina Messai, Houria Ammari, Moufida Hamidi, Rabah Bakour
No abstract text is available yet for this article.
August 29, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30149195/complete-and-assembled-genome-sequence-of-an-ndm-5-and-ctx-m-15-producing-escherichia-coli-sequence-type-617-isolated-from-wastewater-in-switzerland
#10
Zurfluh Katrin, Marc J A Stevens, Roger Stephan, Magdalena Nüesch-Inderbinen
OBJECTIVES: Carbapenem-resistant Escherichia coli have emerged worldwide and represent a major challenge to effective health care management. Here, we report the genome sequence of an NDM-5 and CTX-M-15 producing E. coli belonging to sequence type 617, isolated from wastewater treatment plant effluent in Switzerland. METHODS: Whole genome sequencing of E. coli 657SK2 was performed using Pacific Biosciences (PacBio) single-molecule real-time (SMRT) technology RS2 reads (C4/P6 chemistry)...
August 24, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30149194/changes-in-resistance-to-and-antimicrobial-activity-of-antibiotics-during-in-vitro-human-digestion
#11
Seung Yun Lee, On You Kim, Sung Yeoul Yoon, Da Young Lee, Sun Jin Hur
OBJECTIVES: This study was conducted to determine the changes in antimicrobial activity of antibiotics (tetracycline, ofloxacin, and penicillin) and in resistance of Staphylococcus aureus RN 4220 (SA RN4220) and methicillin-resistant Staphylococcus aureus (MRSA) to these antibiotics during in vitro human digestion. METHODS: A human gastrointestinal digestion model that simulates the conditions of the mouth, stomach, small intestine, and large intestine (with enterobacteria application) was used in this study...
August 24, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30149193/antimicrobial-resistant-cc17-enterococcus-faecium-the-past-the-present-and-the-future
#12
REVIEW
Terence Lee, Stanley Pang, Sam Abraham, Geoffrey W Coombs
Enterococcus faecium are robust opportunistic pathogens that are most commonly found as commensals of the human and animal gut but can also survive in the environment. Since the introduction and use of antimicrobials, E. faecium have been found to rapidly acquire resistance genes which when expressed can effectively circumvent the effects of most antimicrobials. The rapid acquisition of multiple antimicrobial resistances has led to the adaptation of specific E. faecium clones in the hospital environment collectively known as clonal complex (CC)17...
August 24, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30145271/determinants-of-mrsa-prevalence-in-the-asia-pacific-region-a-systematic-review-and-meta-analysis
#13
REVIEW
Wey Wen Lim, Peng Wu, Helen S Bond, Jessica Y Wong, Kaiwen Ni, Wing Hong Seto, Mark Jit, Benjamin J Cowling
OBJECTIVE: We aimed to review published literature on methicillin resistant S. aureus (MRSA) in the Asia Pacific Region to document MRSA prevalence in the Region and examine the impact of variability in study design on the reported data of MRSA prevalence. METHODS: We included studies that reported MRSA prevalence between 2000 and 2016 and excluded studies if they did not contain complete information on antibiotic susceptibility testing (AST) methods Our primary outcomes were the proportion of MRSA isolates among S...
August 23, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30144638/extensively-drug-resistant-and-pan-drug-resistant-gram-negative-bacteria-in-a-tertiary-care-hospital-in-eastern-india-a-4-year-retrospective-study
#14
Debi Prasad Mohapatra, Nagen Kumar Debata, Santosh Kumar Singh
BACKGROUND: Bacteria resistant to different classes of antimicrobial agents are a major threat to humanity and risk leading the world towards the return of the pre-antimicrobial era. This study was undertaken to detect the incidence of extensively drug-resistant (XDR), and pan-drug-resistant (PDR) bacteria isolated in a tertiary care hospital, Bhubaneswar, Odisha. METHODS: Positive bacterial cultures from different clinical samples were identified by VITEK® 2 compact system and antibiotic susceptibility profile of different gram-negative bacteria was analyzed...
August 22, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30144637/prevalence-and-characterization-of-third-generation-cephalosporin-resistant-shigella-flexneri-isolates-from-jiangsu-of-china-2013-2015
#15
Guoye Liu, Huimin Qian, Bo Tang, Yin Chen, Haiquan Kang, Fei Jiang, Ping Ma, Bing Gu, Xiang Huo
OBJECTIVES: To assess the prevalence of Shigella flexneri resistance to third-generation cephalosporins and characterize the underlying mechanism. METHODS: A total of 282S. flexneri strains isolated between 2013 and 2015 in Jiangsu Province of China were bacterially identified, serologically typed and analyzed for their susceptibility to third-generation cephalosporins. The TEM, SHV, OXA-1-like and CTX-M-type ESBL genes were amplified and sequenced. RESULTS: Of the 282S...
August 22, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30144636/acinetobacter-in-veterinary-medicine-with-emphasis-on-a-baumannii
#16
REVIEW
J H van der Kolk, A Endimiani, C Graubner, V Gerber, V Perreten
Acinetobacter spp. are aerobic, rod-shaped Gram-negative bacteria belonging to the Moraxellaceae family of the class Gammaproteobacteria and are considered ubiquitous organisms. Among them, Acinetobacter baumannii is the most clinically significant species with an extraordinary ability to accumulate antimicrobial resistance and survive in the hospital environment. Recent reports indicate that A. baumannii has also evolved into a veterinary nosocomial pathogen. Although Acinetobacter spp. can be identified to species level by the use of the matrix-assisted laser ionization time-of-flight mass spectrometry (MALDI-TOF MS) coupled with an updated database, molecular techniques are still necessary for genotyping and determination of clonal lineages...
August 22, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30144635/prevalence-of-methicillin-resistant-staphylococcus-aureus-colonization-among-surgical-health-care-workers-at-a-tertiary-care-hospital-in-southeastern-china
#17
Yi Kong, Jiaxin Ye, Wanqing Zhou, Yihong Jiang, Hongyi Lin, Xianpin Zhang, Jing Qian, Yaying Zhang, Hai Ge, Yang Li
OBJECTIVES: The aim of this study was to investigate the carriage rates of Staphylococcus aureus and MRSA among surgical HCWs at a tertiary care hospital in southeastern China and analyze the epidemiological relatedness of S. aureus isolates. METHODS: Two hundred and forty-two pharynx swabs were collected between March and April 2016 from 242 HCWs working in seven different wards and departments. The isolates were identified as S. aureus based on morphology, coagulase test, and VITEK test...
August 22, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30130640/whole-genome-sequencing-and-single-nucleotide-polymorphisms-in-multi-drug-resistant-clinical-isolates-of-mycobacterium-tuberculosis-from-the-philippines
#18
Marylette B Roa, Francis A Tablizo, El King D Morado, Lovette F Cunanan, Iris Diana C Uy, Kamela Charmaine S Ng, Karen G Manalastas-Cantos, Joeriggo M Reyes, Sharie Keanne C Ganchua, Concepcion F Ang, Midori Kato-Maeda, Adithya Cattamanchi, Ulas Karaoz, Raul V Destura, Arturo O Lluisma
OBJECTIVES: Thousands of cases of multi-drug resistant Mycobacteria tuberculosis (MTB) have been observed in the Philippines but studies on the genotypes that underlie the observed drug resistance profiles have been lacking. This study aimed to analyse whole genomes of clinical isolates of MTB representing varying resistance profiles to identify single nucleotide polymorphisms (SNPs) in resistance-associated genes. METHODS: The genomes of ten MTB isolates cultured from banked sputum sources were sequenced...
August 18, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30121345/high-prevalence-of-plasmodium-falciparum-antimalarial-drug-resistance-markers-in-isolates-from-asymptomatic-patients-from-the-republic-of-the-congo-between-2010-and-2015
#19
Dominique Fatima Voumbo-Matoumona, Jean Akiana, Marylin Madamet, Lady Charlène Kouna, Jean Bernard Lekana-Douki, Bruno Pradines
OBJECTIVES: This study investigated the prevalence of haplotypes of the Pfdhps, Pfdhfr, Pfcrt, Pfmdr1 and PfK13 resistance markers in isolates from asymptomatic patients from the Republic of the Congo following implementation of artemisinin based-combination therapy (ACT). METHODS: Peripheral blood was collected from asymptomatic children in 2010 and 2015 from Brazzaville in the south and in 2013 in the north of the Congo. Genotypes of Pfmdr1, Pfcrt, Pfdhps, Pfdhfr and PfK13 were assessed by PCR...
August 16, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30121344/antimicrobial-resistance-and-outcomes-of-community-onset-bacterial-bloodstream-infection-in-patients-with-type-2-diabetes
#20
Chung-Huei Huang, Cheng-Hsun Chiu, I-Wen Chen, Shih-Yuan Hung, Cheng-Wei Lin, Brend Ray-Sea Hsu, Yu-Yao Huang
OBJECTIVES: Antimicrobial resistance of bacterial pathogens in diabetic population with infection is seldom investigated. This study evaluated the antimicrobial-resistant bacterial pathogens and the outcomes of community-onset bloodstream infections (COBSIs) in patients with diabetes. METHODS: From year 2007 to 2014, 1271 monomicrobial COBSIs and 178 polymicrobial COBSIs were identified for patients with type 2 diabetes from three teaching hospitals located in Taiwan...
August 16, 2018: Journal of Global Antimicrobial Resistance
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