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Journal of Global Antimicrobial Resistance

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https://www.readbyqxmd.com/read/30447337/antibiotic-resistance-of-escherichia-coli-from-outpatient-urinary-tract-infection-in-women-in-six-european-countries-including-russia
#1
Sofia Ny, Petra Edquist, Uga Dumpis, Kirsi Gröndahl-Yli-Hannuksela, Julia Hermes, Anna-Maria Kling, Anja Klingeberg, Roman Kozlov, Owe Källman, Danuta O Lis, Monika Pomorska-Wesołowska, Māra Saule, Karin Tegmark Wisell, Jaana Vuopio, Ivan Palagin
OBJECTIVES: In the Northern Dimension Antibiotic Resistance Study Finland, Germany, Latvia, Poland, Russia and Sweden investigated the resistance levels of Escherichia coli (E. coli) isolated from urine of outpatient women (18-65 years) that had symptoms of uncomplicated UTI by collecting samples in each respective nation. METHODS: In total 775 E. coli isolates from 1278 clinical urinary samples were collected from October 2015-January 2017. Susceptibility testing was performed and results were interpreted according to the EUCAST criteria...
November 14, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30445212/carbapenemase-producing-acinetobacter-spp-from-environmental-sources-in-a-hospital-from-french-polynesia
#2
LETTER
Rémy A Bonnin, Marc Levy, Gaelle Cuzon, Laurent Dortet, Thierry Naas
No abstract text is available yet for this article.
November 13, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30445211/the-frequency-of-dna-gyrase-and-topoisomerase-iv-mutations-and-plasmid-mediated-quinolone-resistance-genes-among-escherichia-coli-and-klebsiella-pneumoniae-isolated-from-urinary-tract-infections-in-azerbaijan-iran
#3
Robab Azargun, Mohammad Hossein Soroush Barhaghi, Hossein Samadi Kafil, Mahin Ahangar Oskouee, Vahid Sadeghi, Mohammad Yousef Memar, Reza Ghotaslou
OBJECTIVES: The aims of the study were to assess genetic alterations in gyrA, gyrB, parC and parE genes and the prevalence of plasmid-mediated quinolone resistance (PMQR) among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections (UTIs) in Azerbaijan, Iran. METHODS: A total of 205 clinical isolates of E. coli (n=177) and K. pneumoniae (n=28) were obtained from UTIs. The antimicrobial susceptibility determined by the disk diffusion agar and the agar dilution assays...
November 13, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30445210/draft-genome-sequence-of-a-oxa-23-oxa-66-adc-25-and-tem-1d-co-producing-acinetobacter-baumannii-st195-isolated-from-a-patient-with-neonatal-pneumonia
#4
Weibiao Lv, Xiufeng Zhang, Mengtian Hou, Dongdong Han, Yun Li, Wenguang Xiong
OBJECTIVES: The rapid spread of multiple drug-resistant Acinetobacter baumannii (A. baumannii) poses a substantial threat tomorbidity and mortality worldwide. Specifically, carbapenem-resistant A. baumannii has brought a severe challenge on human health. In this study, we reported the draft genome sequence of A. baumannii S131434, an OXA-23, OXA-66, ADC-25 and TEM-1D co-producing strain recovered from a patient with neonatal pneumonia in China and belonging to the globally disseminated clonal sequence type 195 (ST195)...
November 13, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30445209/antimicrobial-susceptibility-of-gram-negative-and-gram-positive-bacteria-collected-from-eastern-europe-results-from-the-tigecycline-evaluation-and-surveillance-trial-t-e-s-t-2011-2016
#5
Michael J Dowzicky, Eva Chmelařová
OBJECTIVES: The Tigecycline Evaluation and Surveillance Trial (T.E.S.T.) is a global surveillance program that monitors the in vitro activity of a panel of antimicrobials against clinically important isolates. Data for Gram-positive and Gram-negative isolates collected in Eastern Europe between 2011 and 2016 are presented. METHODS: MICs were determined by broth microdilution using CLSI guidelines. Antimicrobial susceptibility was determined using European Committee on Antimicrobial Susceptibility Testing breakpoints...
November 13, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30445208/characterization-of-chromosomally-located-bla-ctx-m-and-its-surrounding-sequence-in-ctx-m-type-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolates
#6
Kouta Hamamoto, Itaru Hirai
OBJECTIVES: Although it has been regarded that CTX-M type extended-spectrum β-lactamase (ESBL) gene, blaCTX-M , is mainly carried by antibiotic-resistance plasmids, Escherichia coli-possessing chromosomally-located blaCTX-M has been reported in previous studies. This study aimed to characterize the genetic structures of chromosomally-located blaCTX-M transposition unit and its surrounding sequence in E. coli isolates-producing ESBL which were obtained in a Japanese hospital. METHODS: In this study, total of 81 ESBL-producing E...
November 13, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30445207/molecular-epidemiology-and-genetic-characterization-of-carbapenem-resistant-acinetobacter-baumannii-isolates-from-guangdong-province-south-china
#7
Lili Jiang, Youcai Liang, Wei Yao, Juntao Ai, Xin Wang, Zuguo Zhao
OBJECTIVE: Carbapenem-resistant Acinetobacter baumannii (CRAB) infection has become a worldwide issue. This study aimed to characterize the epidemiology and genetic relationships of A. baumannii in Guangdong Province, China. METHODS: CRAB isolates were collected from five municipal hospitals from June to December 2017. 16S-23S rRNA intergenic spacer(ITS) was used for further strain identification. Antimicrobial susceptibility test and CarbAcineto NP test were performed to analyse antibiotic spectrum and carbapenemase production of those isolates, while PCR-based assays were used to detect beta-lactamase genes and related mobile genetic elements...
November 13, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30412782/carbapenem-resistant-acinetobacter-baumannii-current-status-of-the-problem-in-four-bulgarian-university-hospitals-2014-2016
#8
Tanya Strateva, Ivo Sirakov, Temenuga Stoeva, Alexander Stratev, Svetoslav Dimov, Encho Savov, Ivan Mitov
OBJECTIVES: A total of 226 carbapenem-resistant Acinetobacter baumannii (CRAB) isolates was collected during 2014-2016 from in-patients aged 5-88 years in four Bulgarian university hospitals (H1-H4), to assess their antimicrobial susceptibility and to explore carbapenem resistance mechanisms, as well as the molecular epidemiology. METHODS: Antimicrobial susceptibility testing, multiplex polymerase chain reaction (PCR) assay, DNA sequencing, and electrotransformation experiments were performed...
November 6, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30412781/incidence-of-single-drug-multidrug-and-extensively-drug-antimicrobial-resistance-of-escherichia-coli-urinary-tract-infections-an-australian-laboratory-based-retrospective-study
#9
Oyebola Fasugba, Anindita Das, George Mnatzaganian, Brett G Mitchell, Peter Collignon, Anne Gardner
OBJECTIVES: To evaluate incidence of single-drug resistant, multidrug-resistant, extensively drug-resistant and pandrug-resistant E. coli urinary tract infections (UTI) in a sample of Australian Capital Territory (ACT) residents. METHODS: We utilised laboratory-based retrospective data from all ACT residents whose urine samples were processed from January 2009 to December 2013. Multivariate logistic regression models were constructed to determine the associations of age, sex, urine sample source and socioeconomic status with risk of resistant infections...
November 6, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30404045/genomic-features-of-a-highly-virulent-ceftiofur-resistant-ctx-m-8-producing-escherichia-coli-st224-causing-fatal-infection-in-a-domestic-cat
#10
Meire M Silva, Fábio P Sellera, Miriam R Fernandes, Quézia Moura, Felício Garino, Sérgio S Azevedo, Nilton Lincopan
OBJECTIVES: Escherichia coli strains producing extended-spectrum β-lactamases (ESBL), especially of CTX-M type, have been largely described in companion animals; however genomic data are lacking to clarify the clinical impact of ESBL producers in these hosts. The aim of this study was to present genomic features of a highly virulent ceftiofur-resistant CTX-M-8-producing E. coli lineage from a case of pneumonia in cat with fatal outcome. METHODS: Genomic DNA was sequenced using an Illumina NextSeq500 platform and assembled by CLC Genomic Workbench...
November 4, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30404044/genomic-analysis-of-mcr-1-and-ctx-m-8-co-producing-escherichia-coli-st58-isolated-from-a-polluted-mangrove-ecosystem-in-brazil
#11
Andrey G Sacramento, Miriam R Fernandes, Fábio P Sellera, Maria E Muñoz, Roberto Vivas, Silvio S Dolabella, Nilton Lincopan
INTRODUCTION: Genomic surveillance studies monitoring the dissemination of multidrug resistant (MDR) Enterobacteriaceae in polluted aquatic ecosystems are urgently required. OBJECTIVE: To report the draft genome sequence of a MCR-1 and CTX-M-8 co-producing Escherichia coli isolated from a polluted mangrove ecosystem in Northeast Brazil. METHODS: Total genomic DNA was sequenced on an Illumina NextSeq platform and assembled by using CLC Genomics Workbench...
November 4, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30395969/st648-pandemic-escherichia-coli-lineage-harbouring-fosa3-and-bla-ctx-m-8-on-an-inci1-st113-plasmid-a-new-successful-combination-for-the-spread-of-fosfomycin-resistance
#12
Josman Dantas Palmeira, Helena Ferreira, Jean-Yves Madec, Marisa Haenni
OBJECTIVES: The emergence of Enterobacteriaceae isolates resistant to the last-resort fosfomycin antibiotic outside Asia is a public health issue. Here, we report the draft genome of an E. coli presenting both an Extended-Spectrum Beta-Lactamase (ESBL) and the fosA3 gene in a healthy cow in Brazil. METHODS: Whole genomic DNA from E. coli E12 was extracted and 2×150-bp paired-end reads were generated with Illumina sequencing technology. De novo genome assembly was performed using SPAdes v...
November 2, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30391398/lack-of-correlation-between-reduced-outpatient-consumption-of-macrolides-and-resistance-of-invasive-streptococcus-pneumoniae-isolates-in-slovenia-during-1997-to-2017
#13
Tamara Kastrin, Metka Paragi, Vanja Erčulj, Tjaša Žohar Čretnik, Tom Bajec, Milan Čižman
OBJECTIVES: The objectives of our study were to investigate the correlation between decreased national consumption of macrolides and resistance of invasive Streptococcus pneumoniae isolates in Slovenia during the 1997 to 2017. METHODS: A total of 4241 invasive isolates of S. pneumoniae were collected in Slovenia from 1997 to 2017. In 612 erythromycin-resistant isolates the presence of erm(B), mef(E), mef(A) and erm(TR) genes were determined by PCR. Selected isolates carrying the mef(A) gene were further examined by PFGE...
October 31, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30389638/aminoglycoside-modifying-enzymes-and-16s-ribossomal-rna-methyltransferases-encoding-genes-among-a-global-collection-of-gram-negative-isolates
#14
Sarah E Costello, Lalitagauri M Deshpande, Andrew P Davis, Rodrigo E Mendes, Mariana Castanheira
OBJECTIVES: We evaluated the prevalence of genes encoding aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methyltransferases among 200 Gram-negative clinical isolates resistant to different aminoglycosides and collected worldwide during 2013. METHODS: Selected AMEs and 16S rRNA methyltransferases genes were screened by PCR/sequencing among 49 Acinetobacter spp., 52 Pseudomonas aeruginosa, and 99 Enterobacterales. RESULTS: Seventy-two isolates carried aac(6')-lb variants (36...
October 30, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30389637/staphylococcus-aureus-at-an-indian-tertiary-hospital-antimicrobial-susceptibility-and-minimum-inhibitory-concentration-creep-of-antimicrobials
#15
Surbhi Khurana, Purva Mathur, Rajesh Malhotra
BACKGROUND: Staphylococcus aureus causes a variety of manifestations and diseases and has been associated with a high morbidity and mortality rate. A global population drift in the clinical isolates of S. aureus towards reduced antibiotic susceptibility is being reported. Thus, we studied the antimicrobial susceptibility profile and minimum inhibitory concentration (MIC) creep of vancomycin, linezolid, teicoplanin and rifampicin against clinical isolates of Staphylococcus aureus at an Indian tertiary centre from January 2012 to December 2016...
October 30, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30389636/changing-drug-resistance-profile-in-pseudomonas-aeruginosa-infection-among-hiv-patients-from-2010-2017-a-retrospective-study
#16
Chinnambedu Ravichandran Swathirajan, Marimuthu Ragavan Rameshkumar, Sunil Suhas Solomon, Ramachandran Vignesh, Pachamuthu Balakrishnan
OBJECTIVES: Pseudomonas aeruginosa, an important etiological agent causing pneumonia, urinary tract infections, and bacteraemia. High use of antibiotics in nosocomial settings and during immunocompromised conditions results in increasing multidrug resistance. This study analyzes the antibiotic resistance profile of P.aeruginosa in a HIV setting. METHODS: A total of 7386 clinical specimens were collected from HIV patients attending YRG CARE from the year 2010 to 2017...
October 30, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30367993/azole-resistant-aspergillus-fumigatus-in-the-italian-environment
#17
Anna Prigitano, Maria C Esposto, Luisa Romanò, Francesco Auxilia, Anna M Tortorano
OBJECTIVES: Azole resistance in Aspergillus fumigatus environmental and clinical isolates is recognized as an emerging problem worldwide. The development of azole resistance may be environmentally driven because of the massive use of azole fungicides in agriculture and the mechanism of resistance is mostly related to mutations in the cyp51A gene. A. fumigatus azole resistance in the environment was previously documented in Northern Italy. The present study extended the research in the agricultural environment also in central and southern Italy and investigated the differences in the Italian geographical areas and in the different types of crops...
October 24, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30366149/draft-genome-sequence-of-a-methicillin-resistant-staphylococcus-epidermidis-isolate-from-swine
#18
Luria Leslie Founou, Raspail Carrel Founou, Mushal Allam, Arshad Ismail, Sabiha Yusuf Essack
OBJECTIVES: Here we report the draft genome sequence of a methicillin-resistant Staphylococcus epidermidis strain (sequence type 59) isolated from a pooled rectal sample from pigs collected in an abattoir in South Africa. METHODS: Genomic DNA of S. epidermidis PR246B0 was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using CLC Genomics Workbench (QIAGEN). The assembled contigs were annotated and antimicrobial resistance genes, plasmids and the sequence type were identified...
October 23, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30342929/incfii-plasmid-carrying-antimicrobial-resistance-genes-in-shigella-flexneri-vehicle-for-dissemination
#19
Dhiviya Prabaa Muthuirulandi Sethuvel, Shalini Anandan, Naveen Kumar Devanga Ragupathi, Revathi Gajendiran, Makoto Kuroda, Keigo Shibayama, Balaji Veeraraghavan
OBJECTIVES: Plasmids harboring antimicrobial resistance determinants in clinical strains are the significant public health concern worldwide. The present study investigated for such plasmids in clinical Shigella flexneri isolates. METHODS: Totally, 162 Shigella isolates were obtained from stool specimen for the year 2015. Of the 70 multi-drug resistant Shigella spp., 27S. flexneri isolates were randomly selected for further characterization. Antimicrobial resistance genes and incompatibility plasmid types were analysed...
October 18, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/30342928/emerging-metronidazole-resistance-in-bacteroides-spp-and-its-association-with-nim-gene-a-study-from-north-india
#20
Shveta Sethi, Ratnakar Shukla, Kiran Bala, Vikas Gautam, Archana Angrup, Pallab Ray
BACKGROUND: Bacteroides spp., an anaerobic resident intestinal flora is also known to cause severe morbidity. They are resistant to a wide variety of antimicrobial agents including metronidazole. Specific nitroimidazole (nim) resistance genes have been shown to be associated with the same. Worldwide, metronidazole resistance is emerging, though presently remains around 5%. This study was aimed at metronidazole susceptibility and distribution of nim genes within the Bacteroides spp. We also analyzed the relationship among the strains harboring nim genes and their susceptibility to metronidazole...
October 18, 2018: Journal of Global Antimicrobial Resistance
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