journal
MENU ▼
Read by QxMD icon Read
search

Technology

journal
https://www.readbyqxmd.com/read/30057935/automated-end-to-end-blood-testing-at-the-point-of-care-integration-of-robotic-phlebotomy-with-downstream-sample-processing
#1
M L Balter, J M Leipheimer, A I Chen, A Shrirao, T J Maguire, M L Yarmush
Diagnostic blood testing is the most commonly performed clinical procedure in the world, and influences the majority of medical decisions made in hospital and laboratory settings. However, manual blood draw success rates are dependent on clinician skill and patient physiology, and results are generated almost exclusively in centralized labs from large-volume samples using labor-intensive analytical techniques. This paper presents a medical device that enables end-to-end blood testing by performing blood draws and providing diagnostic results in a fully automated fashion at the point-of-care...
June 2018: Technology
https://www.readbyqxmd.com/read/29682599/microfluidic-flow-cytometry-the-role-of-microfabrication-methodologies-performance-and-functional-specification
#2
Anil B Shrirao, Zachary Fritz, Eric M Novik, Gabriel M Yarmush, Rene S Schloss, Jeffrey D Zahn, Martin L Yarmush
Flow cytometry is an invaluable tool utilized in modern biomedical research and clinical applications requiring high throughput, high resolution particle analysis for cytometric characterization and/or sorting of cells and particles as well as for analyzing results from immunocytometric assays. In recent years, research has focused on developing microfluidic flow cytometers with the motivation of creating smaller, less expensive, simpler, and more autonomous alternatives to conventional flow cytometers. These devices could ideally be highly portable, easy to operate without extensive user training, and utilized for research purposes and/or point-of-care diagnostics especially in limited resource facilities or locations requiring on-site analyses...
March 2018: Technology
https://www.readbyqxmd.com/read/29744376/cell-sized-lipid-vesicles-for-cell-cell-synaptic-therapies
#3
D Vallejo, S H Lee, D Lee, C Zhang, C Rapier, S D Chessler, A P Lee
Cell-sized lipid vesicles (CLVs) have shown great promise for therapeutic and artificial cell applications, but their fragility and short shelf life has hindered widespread adoption and commercial viability. We present a method to circumvent the storage limitations of CLVs such as giant unilamellar vesicles (GUVs) and single-compartment multisomes (SCMs) by storing them in a double emulsion precursor form. The double emulsions can be stored for at least 8 months and readily converted into either GUVs or SCMs at any time...
December 2017: Technology
https://www.readbyqxmd.com/read/29541655/a-thermoreversible-photocrosslinkable-collagen-bio-ink-for-free-form-fabrication-of-scaffolds-for-regenerative-medicine
#4
Kathryn E Drzewiecki, Juilee N Malavade, Ijaz Ahmed, Christopher J Lowe, David I Shreiber
As a biomaterial, collagen has been used throughout tissue engineering and regenerative medicine. Collagen is native to the body, is highly biocompatible, and naturally promotes cell adhesion and regeneration. However, collagen fibers and the inherent weak mechanical properties of collagen hydrogels interfere with further development of collagen as a bio-ink. Herein, we demonstrate the use of a modified type-I collagen, collagen methacrylamide (CMA), as a fibril-forming bio-ink for free-form fabrication of scaffolds...
December 2017: Technology
https://www.readbyqxmd.com/read/29780857/multi-omic-network-based-interrogation-of-rat-liver-metabolism-following-gastric-bypass-surgery-featuring-swath-proteomics
#5
Gautham Vivek Sridharan, Matthew D'Alessandro, Shyam Sundhar Bale, Vicky Bhagat, Hugo Gagnon, John M Asara, Korkut Uygun, Martin L Yarmush, Nima Saeidi
Morbidly obese patients often elect for Roux-en-Y gastric bypass (RYGB), a form of bariatric surgery that triggers a remarkable 30% reduction in excess body weight and reversal of insulin resistance for those who are type II diabetic. A more complete understanding of the underlying molecular mechanisms that drive the complex metabolic reprogramming post-RYGB could lead to innovative non-invasive therapeutics that mimic the beneficial effects of the surgery, namely weight loss, achievement of glycemic control, or reversal of non-alcoholic steatohepatitis (NASH)...
September 2017: Technology
https://www.readbyqxmd.com/read/29552603/hypoxia-impairs-mesenchymal-stromal-cell-induced-macrophage-m1-to-m2-transition
#6
Renea A Faulknor, Melissa A Olekson, Emmanuel C Ekwueme, Paulina Krzyszczyk, Joseph W Freeman, François Berthiaume
The transition of macrophages from the pro-inflammatory M1 to the anti-inflammatory M2 phenotype is crucial for the progression of normal wound healing. Persistent M1 macrophages within the injury site may lead to an uncontrolled macrophage-mediated inflammatory response and ultimately a failure of the wound healing cascade, leading to chronic wounds. Mesenchymal stromal cells (MSCs) have been widely reported to promote M1 to M2 macrophage transition; however, it is unclear whether MSCs can drive this transition in the hypoxic environment typically observed in chronic wounds...
June 2017: Technology
https://www.readbyqxmd.com/read/28804751/ultrasound-imaging-and-segmentation-of-bone-surfaces-a-review
#7
Ilker Hacihaliloglu
Due to its real-time, non-radiation based three-dimensional (3D) imaging capabilities, ultrasound (US) has been incorporated into various orthopedic procedures. However, imaging artifacts, low signal-to-noise ratio (SNR) and bone boundaries appearing several mm in thickness make the analysis of US data difficult. This paper provides a review about the state-of-the-art bone segmentation and enhancement methods developed for two-dimensional (2D) and 3D US data. First, an overview for the appearance of bone surface response in B-mode data is presented...
June 2017: Technology
https://www.readbyqxmd.com/read/28781994/rapid-antibiotic-sensitivity-testing-in-microwell-arrays
#8
Fatemeh Jalali, Felix Ellett, Daniel Irimia
The widespread bacterial resistance to a broad range of antibiotics necessitates rapid antibiotic susceptibility testing before effective treatment could start in the clinic. Among resistant bacteria, Staphylococcus aureus is one of the most important, and Methicillin-resistant (MRSA) strains are a common cause of life threatening infections. However, standard susceptibility testing for S. aureus is time consuming and thus the start of effective antibiotic treatment is often delayed. To circumvent the limitations of current susceptibility testing systems, we designed an assay that enables measurements of bacterial growth with higher spatial and temporal resolution than standard techniques...
June 2017: Technology
https://www.readbyqxmd.com/read/28781993/%C3%AE-neurocircuitry-establishing-in-vitro-models-of-neurocircuits-with-human-neurons
#9
Joseph A Fantuzzo, Lidia De Filippis, Heather McGowan, Nan Yang, Yi-Han Ng, Apoorva Halikere, Jing-Jing Liu, Ronald P Hart, Marius Wernig, Jefrey D Zahn, Zhiping P Pang
Neurocircuits in the human brain govern complex behavior and involve connections from many different neuronal subtypes from different brain regions. Recent advances in stem cell biology have enabled the derivation of patient-specific human neuronal cells of various subtypes for the study of neuronal function and disease pathology. Nevertheless, one persistent challenge using these human-derived neurons is the ability to reconstruct models of human brain circuitry. To overcome this obstacle, we have developed a compartmentalized microfluidic device, which allows for spatial separation of cell bodies of different human-derived neuronal subtypes (excitatory, inhibitory and dopaminergic) but is permissive to the spreading of projecting processes...
June 2017: Technology
https://www.readbyqxmd.com/read/28713851/building-an-experimental-model-of-the-human-body-with-non-physiological-parameters
#10
Joseph M Labuz, Christopher Moraes, David R Mertz, Brendan M Leung, Shuichi Takayama
New advances in engineering and biomedical technology have enabled recent efforts to capture essential aspects of human physiology in microscale, in-vitro systems. The application of these advances to experimentally model complex processes in an integrated platform - commonly called a 'human-on-a-chip (HOC)' - requires that relevant compartments and parameters be sized correctly relative to each other and to the system as a whole. Empirical observation, theoretical treatments of resource distribution systems and natural experiments can all be used to inform rational design of such a system, but technical and fundamental challenges (e...
March 2017: Technology
https://www.readbyqxmd.com/read/28695160/recent-advances-in-nonbiofouling-pdms-surface-modification-strategies-applicable-to-microfluidic-technology
#11
Aslihan Gokaltun, Martin L Yarmush, Ayse Asatekin, O Berk Usta
In the last decade microfabrication processes including rapid prototyping techniques have advanced rapidly and achieved a fairly mature stage. These advances have encouraged and enabled the use of microfluidic devices by a wider range of users with applications in biological separations and cell and organoid cultures. Accordingly, a significant current challenge in the field is controlling biomolecular interactions at interfaces and the development of novel biomaterials to satisfy the unique needs of the biomedical applications...
March 2017: Technology
https://www.readbyqxmd.com/read/28674697/cell-free-vascular-grafts-recent-developments-and-clinical-potential
#12
Sindhu Row, Ana Santandreu, Daniel D Swartz, Stelios T Andreadis
Recent advances in vascular tissue engineering have led to the development of cell-free grafts that are available off-the-shelf for on demand surgery. Challenges associated with cell-based technologies including cell sourcing, cell expansion and long-term bioreactor culture motivated the development of completely cell-free vascular grafts. These are based on decellularized arteries, decellularized cultured cell-based tissue engineered grafts or biomaterials functionalized with biological signals that promote in situ tissue regeneration...
March 2017: Technology
https://www.readbyqxmd.com/read/28317005/a-cost-effective-micromilling-platform-for-rapid-prototyping-of-microdevices
#13
Daniel P Yen, Yuta Ando, Keyue Shen
Micromilling has great potential in producing microdevices for lab-on-a-chip and organ-on-a-chip applications, but has remained under-utilized due to the high machinery costs and limited accessibility. In this paper, we assessed the machining capabilities of a low-cost 3-D mill in polycarbonate material, which were showcased by the production of microfluidic devices. The study demonstrates that this particular mill is well suited for the fabrication of multi-scale microdevices with feature sizes from micrometers to centimeters...
December 2016: Technology
https://www.readbyqxmd.com/read/29732384/donor-variability-among-anti-inflammatory-pre-activated-mesenchymal-stromal-cells
#14
Andrea Gray, Rene S Schloss, Martin Yarmush
Therapeutic mesenchymal stromal cells (MSCs) are attractive in part due to their immunomodulatory properties, achieved by their paracrine secretion of factors including prostaglandin E2 (PGE2). Despite promising pre-clinical data, demonstrating clinical efficacy has proven difficult. The current studies were designed to develop approaches to pre-induce desired functions from naïve MSCs and examine MSC donor variability, two factors contributing to this disconnect. MSCs from six human donors were pre-activated with interleukin 1 beta (IL-1β) at a concentration and duration identified as optimal or interferon gamma (IFN-γ) as a comparator...
September 2016: Technology
https://www.readbyqxmd.com/read/28713850/collagen-and-heparan-sulfate-coatings-differentially-alter-cell-proliferation-and-attachment-in-vitro-and-in-vivo
#15
Christopher M Walthers, Chase J Lyall, Alireza K Nazemi, Puneet V Rana, James C Y Dunn
Tissue engineering is an innovative field of research applied to treat intestinal diseases. Engineered smooth muscle requires dense smooth muscle tissue and robust vascularization to support contraction. The purpose of this study was to use heparan sulfate (HS) and collagen coatings to increase the attachment of smooth muscle cells (SMCs) to scaffolds and improve their survival after implantation. SMCs grown on biologically coated scaffolds were evaluated for maturity and cell numbers after 2, 4 and 6 weeks in vitro and both 2 and 6 weeks in vivo ...
September 2016: Technology
https://www.readbyqxmd.com/read/27583301/electropermanent-magnet-actuation-for-droplet-ferromicrofluidics
#16
José I Padovani, Stefanie S Jeffrey, Roger T Howe
Droplet actuation is an essential mechanism for droplet-based microfluidic systems. On-demand electromagnetic actuation is used in a ferrofluid-based microfluidic system for water droplet displacement. Electropermanent magnets (EPMs) are used to induce 50 mT magnetic fields in a ferrofluid filled microchannel with gradients up to 6.4 × 10(4) kA/m(2). Short 50 µs current pulses activate the electropermanent magnets and generate negative magnetophoretic forces that range from 10 to 70 nN on 40 to 80 µm water-in-ferrofluid droplets...
June 2016: Technology
https://www.readbyqxmd.com/read/27437432/dynamic-deformability-of-sickle-red-blood-cells-in-microphysiological-flow
#17
Y Alapan, Y Matsuyama, J A Little, U A Gurkan
In sickle cell disease (SCD), hemoglobin molecules polymerize intracellularly and lead to a cascade of events resulting in decreased deformability and increased adhesion of red blood cells (RBCs). Decreased deformability and increased adhesion of sickle RBCs lead to blood vessel occlusion (vaso-occlusion) in SCD patients. Here, we present a microfluidic approach integrated with a cell dimensioning algorithm to analyze dynamic deformability of adhered RBC at the single-cell level in controlled microphysiological flow...
June 2016: Technology
https://www.readbyqxmd.com/read/27853735/two-stage-nanoparticle-delivery-of-piperlongumine-and-tumor-necrosis-factor-related-apoptosis-inducing-ligand-trail-anti-cancer-therapy
#18
Charles C Sharkey, Jiahe Li, Sweta Roy, Qianhui Wu, Michael R King
This study outlines a drug delivery mechanism that utilizes two independent vehicles, allowing for delivery of chemically and physically distinct agents. The mechanism was utilized to deliver a new anti-cancer combination therapy consisting of piperlongumine (PL) and TRAIL to treat PC3 prostate cancer and HCT116 colon cancer cells. PL, a small-molecule hydrophobic drug, was encapsulated in poly (lactic-co-glycolic acid) (PLGA) nanoparticles. TRAIL was chemically conjugated to the surface of liposomes. PL was first administered to sensitize cancer cells to the effects of TRAIL...
March 2016: Technology
https://www.readbyqxmd.com/read/27785459/signal-transduction-of-the-physical-environment-in-the-neural-differentiation-of-stem-cells
#19
Ryan Thompson, Christina Chan
Neural differentiation is largely dependent on extracellular signals within the cell microenvironment. These extracellular signals are mainly in the form of soluble factors that activate intracellular signaling cascades that drive changes in the cell nucleus. However, it is becoming increasingly apparent that the physical microenvironment provides signals that can also influence lineage commitment and very low modulus surfaces has been repeatedly demonstrated to promote neurogenesis. The molecular mechanisms governing mechano-induced neural differentiation are still largely uncharacterized; however, a growing body of evidence indicates that physical stimuli can regulate known signaling cascades and transcription factors involved in neural differentiation...
March 2016: Technology
https://www.readbyqxmd.com/read/27774502/dextran-functionalization-enhances-nanoparticle-mediated-sirna-delivery-and-silencing
#20
Daniel Vocelle, Olivia M Chesniak, Amanda P Malefyt, Georgina Comiskey, Kwasi Adu-Berchie, Milton R Smith, Christina Chan, S Patrick Walton
Understanding the endocytosis and intracellular trafficking of short interfering RNA (siRNA) delivery vehicle complexes remains a critical bottleneck in designing siRNA delivery vehicles for highly active RNA interference (RNAi)-based therapeutics. In this study, we show that dextran functionalization of silica nanoparticles enhanced uptake and intracellular delivery of siRNAs in cultured cells. Using pharmacological inhibitors for endocytotic pathways, we determined that our complexes are endocytosed via a previously unreported mechanism for siRNA delivery in which dextran initiates scavenger receptor-mediated endocytosis through a clathrin/caveolin-independent process...
March 2016: Technology
journal
journal
48725
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"