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Biomedical Reports

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https://www.readbyqxmd.com/read/28584644/evaluation-of-tumor-necrosis-factor-tnf-%C3%AE-mrna-expression-level-and-the-rs1799964-polymorphism-of-the-tnf-%C3%AE-gene-in-peripheral-mononuclear-cells-of-patients-with-inflammatory-bowel-diseases
#1
Mahyar Nourian, Vahid Chaleshi, Leila Pishkar, Pedram Azimzadeh, Shaghayegh Baradaran Ghavami, Hedieh Balaii, Samaneh Alinaghi, Shabnam Shahrokh, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
Crohn's disease (CD) and ulcerative colitis (UC) are types of chronic inflammatory bowel disease (IBD) of which the actual causes remain unknown. Emerging data indicate that alterations in cytokine synthesis may be involved in IBD pathogenesis. The aim of the present study was to determine whether the tumor necrosis factor (TNF)-α mRNA expression level and rs1799964 polymorphism are the genetic susceptibility component of IBD development. The TNF-α mRNA expression level of peripheral blood mononuclear cells (PBMCs) was measured using comparative reverse-transcription quantitative polymerase chain reaction (PCR)...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584643/suppression-of-inflammation-by-the-rhizome-of-anemarrhena-asphodeloides-via-regulation-of-nuclear-factor-%C3%AE%C2%BAb-and-p38-signal-transduction-pathways-in-macrophages
#2
Ba Reum Kim, Young-Chang Cho, Hien Thi Thu Le, Huong Lan Vuong, Sewoong Lee, Sayeon Cho
The rhizome of Anemarrhena asphodeloides Bunge (A. asphodeloides) has been used as a traditional East Asian medicine for the treatment of various types of inflammatory disease. However, to the best of our knowledge, there have been no systemic studies regarding the molecular mechanisms of action of the A. asphodeloides rhizome anti-inflammatory effects. The aim of the present study was to elucidate the anti-inflammatory effects and underlying mechanism of action of ethanol extracts of the rhizome of A. asphodeloides (EAA) in murine macrophages...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584642/pretreatment-with-intravenous-amiodarone-improves-the-efficacy-of-ibutilide-treatment-on-cardioversion-rate-and-maintenance-time-of-sinus-rhythm-in-patients-with-persistent-atrial-fibrillation
#3
Zengxiang Dong, Hong Yao, Zhuangzhuang Miao, Hao Wang, Rongsheng Xie, Ye Wang, Yingfang Shang, Chunlin Gong, Zhaoguang Liang
The aim of the present study was to assess the efficacy and safety of the pharmacological conversion of persistent atrial fibrillation (AF) using amiodarone or/and ibutilide. Seventy-nine consecutive patients (48 males and 31 females; mean age, 64.6±11.2 years; range, 40-80 years) with non-valvular chronic AF lasting >7 days (range, 7-97 days) that were admitted to hospital for elective pharmacological cardioversion were randomly assigned to receive treatment with intravenous ibutilide (1 mg plus an additional 1 mg if required; n=39) or intravenous amiodarone (300 mg) plus intravenous ibutilide (1 mg; n=40)...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584641/barley-grass-extract-causes-apoptosis-of-cancer-cells-by-increasing-intracellular-reactive-oxygen-species-production
#4
Sang Mi Woo, Sang-Chul Kwon, Seong Gyu Ko, Sung-Gook Cho
Cancer remains a leading cause of mortality worldwide, therefore food products are being investigated for potential prevention or treatment strategies. The ingredient, barley grass extract (Hordeum vulgare L.; Bex) is used to prevent or ameliorate various types of disease. In cancer, Bex has been revealed to inhibit tumor growth. However, its effect on cancer cells is yet to be clearly defined. In the present study, the effect of Bex on cancer cell growth was investigated. Bex inhibited the viabilities of breast and prostate cancer cells according to the results of MTT assays...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584640/chlorpheniramine-and-escitalopram-similar-antidepressant-and-nitric-oxide-lowering-roles-in-a-mouse-model-of-anxiety
#5
Omar Gammoh, Fadia Mayyas, Feras Darwish Elhajji
There is a crosstalk between mood disorders and oxidative stress. Chlorpheniramine (CPA), a first generation antihistamine, is hypothesized to have an anxiolytic role at high doses; however, its antidepressant and antioxidant roles have not previously been investigated. The aim of the current study was to evaluate the antidepressant and anxiolytic effects of CPA treatment in association with nitric oxide (NO) and super oxide dismutase (SOD) activity in a mouse model of anxiety. BALB/c mice were divided into unstressed (naïve), control, and CPA- (0...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584639/the-evaluation-of-fibrotic-effects-of-the-hepatitis-b-virus-pre-core-in-hepatic-stellate-cells
#6
Seyed Younes Hosseini, Kazem Baesi, Negar Azarpira, Ameneh Pakneiat, Seyedeh Akram Hosseini
The role of the hepatitis B virus (HBV) endogenous pre-core protein in liver fibrosis is controversial. Whether the expression of the pre-core induces the activation of human stellate cells (HSCs) has not yet been reported. Plasmids expressing HBx, or pre-core protein were transfected into LX-2 cells. Subsequently, total RNA extracted and reverse transcription-quantitative polymerase chain reaction was performed to measure the fold change of collagen type I, α1 chain, α-smooth muscle actin and TIMP metalloproteinase inhibitor-1...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584638/association-between-angiotensin-converting-enzyme-insertion-deletion-polymorphisms-and-intracranial-aneurysm-susceptibility-a-meta-analysis
#7
Yan-Ping Cun, Cheng-Jie Xiong, Bo Diao, Yu Yang, Li Pan, Lian-Ting Ma
Various studies have evaluated the association between polymorphisms of angiotensin-converting enzyme (ACE) and intracranial aneurysm (IA) risk; however, the results remain inconsistent. The PubMed, Embase, and Wanfang Data databases were systematically searched until January 6th 2016. Case-control studies investigating the association between the ACE polymorphism and IA risk were included. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated with the fixed or random-effects model assuming allele, homozygote comparison of codominant, heterozygote comparison of codominant, dominant, and recessive models...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584637/identification-of-global-transcriptome-abnormalities-and-potential-biomarkers-in-eutopic-endometria-of-women-with-endometriosis-a-preliminary-study
#8
Luyang Zhao, Chenglei Gu, Mingxia Ye, Zhe Zhang, Weidong Han, Wensheng Fan, Yuanguang Meng
The etiology and pathophysiology of endometriosis remain unclear. The aim of the current study was to identify a candidate pathogenic gene, as well as potential biomarkers of endometriosis using messenger RNA (mRNA) sequencing (mRNA-seq). Twenty-three eutopic endometria from women with endometriosis and 20 endometria from control subjects were investigated. Eight eutopic endometria and five normal endometria were selected for mRNA-seq. Differentially expressed genes (DEGs) were identified and functional analysis was conducted...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584636/atp-serves-an-anti-inflammatory-role-by-enhancing-%C3%AE-defensin-2-response-in-acute-pneumonia-of-rat
#9
Zhenwei Shen, Yun Zhou, Le Qu, Han Lei
The aim of the current study was to evaluate the effect of ATP on the expression of rat β-defensin-2 (rBD-2) in a time-dependent manner, as well as its therapeutic value in an acute pneumonia rat model. A total of 30 rats as a treatment group and 30 as a control group were treated with the same dose of ATP and normal saline, respectively, lung tissues were isolated from rat and expression of rBD-2 mRNA was assessed with reverse transcription-quantitative polymerase chain reaction (RT-qPCR) at 12, 24 and 36 h following treatment...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584635/interactions-between-polymorphisms-in-the-3-untranslated-region-of-the-cyclin-dependent-kinase-6-gene-and-the-human-papillomavirus-infection-and-risk-of-cervical-precancerous-lesions
#10
Xingguang Ye, Lipeng Jing, Xingming Zhong, Di Xiao, Meiling Ou, Congcong Guo, Guang Yang, Chunxia Jing, Xiangcai Wei
The human papillomavirus (HPV) infection is essential for the development of cervical cancer and its precursor lesions. However, only certain persistently infected individuals develop cervical cancer. Cyclin-dependent kinase 6 (CDK6) is a critical regulatory cancer-associated gene in the cell cycle and in tumorigenesis. Single nucleotide polymorphisms (SNPs) in microRNA sites in the 3'-untranslated region (UTR) of target genes may result in target gene expression level changes and susceptibility to diseases, including cancer...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584634/analysis-of-the-changes-in-th9-cells-and-related-cytokines-in-the-peripheral-blood-of-spontaneous-urticaria-patients
#11
Renshan Zheng, Lei Qian, Jianxiu Yu, Ming Li, Qing Qian
Spontaneous urticaria (SU) is characterized by immune deregulation of mast cells and T helper (Th) cells. Th9 cells, a subset of Th cells, serve a key role in initiating mast cell accumulation and activation. To understand the role of Th9 cells in the pathogenesis of SU, the authors conducted a control study of 28 patients with acute SU (ASU) and chronic SU (CSU) and 28 healthy controls. The percentage of Th9 cells in peripheral blood was assessed using flow cytometry and levels of Th9 related serum cytokines including interleukin (IL)-4, IL-9, IL-17A, IL-33, IL-1β and transforming growth factor-β1 (TGF-β1) using Luminex 200...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584633/construction-of-a-general-albumin-promoter-reporter-system-for-real-time-monitoring-of-the-differentiation-status-of-functional-hepatocytes-from-stem-cells-in-mouse-rat-and-human
#12
Jing Tang, Qiong Wu, Yi Li, Xiujuan Wu, Yujia Wang, Lihua Zhu, Yujun Shi, Hong Bu, Ji Bao, Mingjun Xie
Genetic constructs with promoters fused to reporter genes for simultaneous monitoring of cellular events have been the focus of attention in recent years. Adenoviral vectors, which have distinctive characteristics, have been used to monitor the differentiation of stem cells in vitro. In the present study, a modified adenoviral vector was constructed, containing a mouse, rat, and human general albumin promoter sequence fused to a ZsGreen reporter gene, and evaluated its efficiency in different cell types. Two hepatocyte cell lines (Hepa1-6 and HepG2), rat primary hepatocytes, rat bone marrow mesenchymal stem cells (BM-MSCs) and rat BM-MSCs-derived hepatocyte-like cells were transduced with this vector, and the transfection efficiency and functional capabilities of the promoter were evaluated by fluorescent microscopy...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584632/biochemical-and-structural-features-of-extracellular-vesicle-binding-rna-aptamers
#13
Kazuyoshi Murakami, Jing Zhao, Kazuhiko Yamasaki, Makoto Miyagishi
Extracellular vesicles are particles in mammalian body fluids that have attracted considerable attention as biomarkers for various diseases. In the present study, the authors isolated RNA aptamers with an affinity for extracellular vesicles from two library pools that encoded randomized sequences of different lengths. After the several rounds of selection, two conserved motifs are identified in the sequences that are obtained by next-generation sequencing. Most of the sequences were predicted to adopt a secondary structure that consisted of a non-conserved stem structure and a conserved loop sequence...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584631/drug-development-lessons-from-nature
#14
Sunil Mathur, Clare Hoskins
Natural products have been acknowledged for numerous years as a vital source of active ingredients in therapeutic agents. In particular, the use of active ingredients derived from plants for use in microbial natural products have long been used before the dawn of modern medicine. From ancient times, the efficacy of natural products has been associated with the chemistry, biochemistry and synthetic activities of natural products. Thus, with scientific advancement in modern molecular and cellular biology, analytical chemistry and pharmacology, the unique properties of these natural products are being harnessed in order to exploit the chemical and structural diversity and biodiversity of these types of products in relation to their therapeutic effect...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584630/connexin-43-key-roles-in-the-skin
#15
Xiao-Fei Zhang, Xiaofeng Cui
Gap junctions are tightly packed intercellular channels that serve a common purpose of allowing the intercellular exchange of small metabolites, second messengers and electrical signals. Connexins (Cxs) are gap junction proteins. Currently, 20 and 21 members of Cxs have been characterized in mice and humans, respectively. Connexin 43 (Cx43) is the most ubiquitously expressed type of Cx in the skin. It is produced by various different types of skin cell, such as keratinocytes, fibroblasts, endothelial and basal cells, melanocytes and dermal papilla cells...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584629/role-of-the-microrna-29-family-in-fibrotic-skin-diseases
#16
Duygu Harmanci, Erdogan Pekcan Erkan, Ayse Kocak, Gul Guner Akdogan
Fibrotic skin diseases are characterized by the accumulation of collagen. The hallmarks of fibrotic skin diseases are unbalanced fibroblast proliferation and differentiation, extracellular matrix production and transforming growth factor-β signalling. Numerous studies have investigated the possibility that microRNAs (miRNAs or miRs) are involved in the pathogenesis of certain fibrotic diseases, including skin, heart, lung and liver diseases. miRNAs are a class of small non-coding RNAs, which modify gene expression by binding to target messenger RNA (mRNA) and blocking the translation or inducing the degradation of target mRNA...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28584628/involvement-of-glycogen-debranching-enzyme-in-bladder-cancer
#17
Benjamin Weinhaus, Sunny Guin
Bladder cancer is the most common malignancy of the urinary system, however the molecular pathways underlying this disease are incompletely understood. To understand new regulators of bladder cancer progression, the authors carried out a functional genomic screen which identified glycogen debranching enzyme (AGL) as a novel regulator of bladder cancer growth. Glycogen debranching enzyme is involved in glycogen breakdown and germline loss of function mutation of this gene leads to glycogen storage disease type III...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28529739/long-term-supplementation-with-young-coconut-juice-does-not-prevent-bone-loss-but-rather-alleviates-body-weight-gain-in-ovariectomized-rats
#18
Hiroshi Matsushita, Akira Minami, Hiroaki Kanazawa, Takashi Suzuki, Sanan Subhadhirasakul, Kazushi Watanabe, Akihiko Wakatsuki
Young coconut (Cocos nucifera Linn.) juice (YCJ) has traditionally been consumed to alleviate symptoms associated with the menopause. Recently, the authors demonstrated that short-term (6-week) YCJ supplementation to ovariectomized rats resulted in increased bone mass and bone formation parameter, suggesting that YCJ consumption has a positive effect on bone metabolism and may represent an intervention to help slow the bone loss during menopause transition. The present study sought to determine how long-term (12-week) YCJ supplementation affects bone metabolism in ovariectomized rats, to investigate whether such supplementation may be helpful to in osteoporosis treatment...
May 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28529738/therapeutic-benefits-of-glutamine-an-umbrella-review-of-meta-analyses
#19
Marc P McRae
Glutamine may be an essential amino acid in patients with catabolic disease, as it has been demonstrated that circulating glutamine levels drop during critical illness and following major surgery; this may result in an increase in secondary infection risk, recovery time and mortality rates. However, there is much discrepancy in the literature with regards to randomized controlled studies, and therefore, the present study is an umbrella review of published meta-analyses, conducted to examine the effectiveness of glutamine's role as a therapeutic agent...
May 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28529737/identifying-the-association-between-interleukin-6-and-lichen-planus-a-meta-analysis
#20
Meng Yin, Guifeng Li, Hui Song, Song Lin
Numerous studies have examined the association between interleukin-6 and the pathogenesis of lichen planus (LP)/oral LP (OLP) in various populations; however, there is a lack of systematic analysis. The aim of the present study was to assess this association more precisely, thus a meta-analysis was performed. Case-control studies, which were published up to December 2015, were obtained from PubMed, Embase and the China National Knowledge Infrastructure databases. Data were extracted and pooled mean differences (MDs) with 95% confidence intervals (CIs) were calculated...
May 2017: Biomedical Reports
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