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Bone Research

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https://www.readbyqxmd.com/read/29644115/shp2-regulates-skeletal-cell-fate-by-modifying-sox9-expression-and-transcriptional-activity
#1
Chunlin Zuo, Lijun Wang, Raghavendra M Kamalesh, Margot E Bowen, Douglas C Moore, Mark S Dooner, Anthony M Reginato, Qian Wu, Christoph Schorl, Yueming Song, Matthew L Warman, Benjamin G Neel, Michael G Ehrlich, Wentian Yang
Chondrocytes and osteoblasts differentiate from a common mesenchymal precursor, the osteochondroprogenitor (OCP), and help build the vertebrate skeleton. The signaling pathways that control lineage commitment for OCPs are incompletely understood. We asked whether the ubiquitously expressed protein-tyrosine phosphatase SHP2 (encoded by Ptpn11 ) affects skeletal lineage commitment by conditionally deleting Ptpn11 in mouse limb and head mesenchyme using "Cre-loxP"-mediated gene excision. SHP2-deficient mice have increased cartilage mass and deficient ossification, suggesting that SHP2-deficient OCPs become chondrocytes and not osteoblasts...
2018: Bone Research
https://www.readbyqxmd.com/read/29644114/super-enhancer-inhibitors-suppress-myc-driven-transcriptional-amplification-and-tumor-progression-in-osteosarcoma
#2
Demeng Chen, Zhiqiang Zhao, Zixin Huang, Du-Chu Chen, Xin-Xing Zhu, Yi-Ze Wang, Ya-Wei Yan, Shaojun Tang, Subha Madhavan, Weiyi Ni, Zhan-Peng Huang, Wen Li, Weidong Ji, Huangxuan Shen, Shuibin Lin, Yi-Zhou Jiang
Osteosarcoma is the most common primary bone sarcoma that mostly occurs in young adults. The causes of osteosarcoma are heterogeneous and still not fully understood. Identification of novel, important oncogenic factors in osteosarcoma and development of better, effective therapeutic approaches are in urgent need for better treatment of osteosarcoma patients. In this study, we uncovered that the oncogene MYC is significantly upregulated in metastastic osteosarcoma samples. In addition, high MYC expression is associated with poor survival of osteosarcoma patients...
2018: Bone Research
https://www.readbyqxmd.com/read/29619270/chip-regulates-bone-mass-by-targeting-multiple-traf-family-members-in-bone-marrow-stromal-cells
#3
Tingyu Wang, Shan Li, Dan Yi, Guang-Qian Zhou, Zhijie Chang, Peter X Ma, Guozhi Xiao, Di Chen
Carboxyl terminus of Hsp70-interacting protein (CHIP or STUB1) is an E3 ligase and regulates the stability of several proteins which are involved in different cellular functions. Our previous studies demonstrated that Chip deficient mice display bone loss phenotype due to increased osteoclast formation through enhancing TRAF6 activity in osteoclasts. In this study we provide novel evidence about the function of CHIP. We found that osteoblast differentiation and bone formation were also decreased in Chip KO mice...
2018: Bone Research
https://www.readbyqxmd.com/read/29619269/omentin-1-prevents-inflammation-induced-osteoporosis-by-downregulating-the-pro-inflammatory-cytokines
#4
Shan-Shan Rao, Yin Hu, Ping-Li Xie, Jia Cao, Zhen-Xing Wang, Jiang-Hua Liu, Hao Yin, Jie Huang, Yi-Juan Tan, Juan Luo, Ming-Jie Luo, Si-Yuan Tang, Tuan-Hui Chen, Ling-Qing Yuan, Er-Yuan Liao, Ran Xu, Zheng-Zhao Liu, Chun-Yuan Chen, Hui Xie
Osteoporosis is a frequent complication of chronic inflammatory diseases and increases in the pro-inflammatory cytokines make an important contribution to bone loss by promoting bone resorption and impairing bone formation. Omentin-1 is a newly identified adipocytokine that has anti-inflammatory effects, but little is known about the role of omentin-1 in inflammatory osteoporosis. Here we generated global omentin-1 knockout ( omentin-1 -/- ) mice and demonstrated that depletion of omentin-1 induces inflammatory bone loss-like phenotypes in mice, as defined by abnormally elevated pro-inflammatory cytokines, increased osteoclast formation and bone tissue destruction, as well as impaired osteogenic activities...
2018: Bone Research
https://www.readbyqxmd.com/read/29619268/enhancer-variants-reveal-a-conserved-transcription-factor-network-governed-by-pu-1-during-osteoclast-differentiation
#5
Heather A Carey, Blake E Hildreth, Jennifer A Geisler, Mara C Nickel, Jennifer Cabrera, Sankha Ghosh, Yue Jiang, Jing Yan, James Lee, Sandeep Makam, Nicholas A Young, Giancarlo R Valiente, Wael N Jarjour, Kun Huang, Thomas J Rosol, Ramiro E Toribio, Julia F Charles, Michael C Ostrowski, Sudarshana M Sharma
Genome-wide association studies (GWASs) have been instrumental in understanding complex phenotypic traits. However, they have rarely been used to understand lineage-specific pathways and functions that contribute to the trait. In this study, by integrating lineage-specific enhancers from mesenchymal and myeloid compartments with bone mineral density loci, we were able to segregate osteoblast- and osteoclast (OC)-specific functions. Specifically, in OCs, a PU.1-dependent transcription factor (TF) network was revealed...
2018: Bone Research
https://www.readbyqxmd.com/read/29581909/mechanically-induced-ca-2-oscillations-in-osteocytes-release-extracellular-vesicles-and-enhance-bone-formation
#6
Andrea E Morrell, Genevieve N Brown, Samuel T Robinson, Rachel L Sattler, Andrew D Baik, Gehua Zhen, Xu Cao, Lynda F Bonewald, Weiyang Jin, Lance C Kam, X Edward Guo
The vast osteocytic network is believed to orchestrate bone metabolic activity in response to mechanical stimuli through production of sclerostin, RANKL, and osteoprotegerin (OPG). However, the mechanisms of osteocyte mechanotransduction remain poorly understood. We've previously shown that osteocyte mechanosensitivity is encoded through unique intracellular calcium (Ca2+ ) dynamics. Here, by simultaneously monitoring Ca2+ and actin dynamics in single cells exposed to fluid shear flow, we detected actin network contractions immediately upon onset of flow-induced Ca2+ transients, which were facilitated by smooth muscle myosin and further confirmed in native osteocytes ex vivo...
2018: Bone Research
https://www.readbyqxmd.com/read/29507819/igf-i-induced-phosphorylation-of-pth-receptor-enhances-osteoblast-to-osteocyte-transition
#7
Tao Qiu, Janet L Crane, Liang Xie, Lingling Xian, Hui Xie, Xu Cao
Parathyroid hormone (PTH) regulates bone remodeling by activating PTH type 1 receptor (PTH1R) in osteoblasts/osteocytes. Insulin-like growth factor type 1 (IGF-1) stimulates mesenchymal stem cell differentiation to osteoblasts. However, little is known about the signaling mechanisms that regulates the osteoblast-to-osteocyte transition. Here we report that PTH and IGF-I synergistically enhance osteoblast-to-osteocyte differentiation. We identified that a specific tyrosine residue, Y494, on the cytoplasmic domain of PTH1R can be phosphorylated by insulin-like growth factor type I receptor (IGF1R) in vitro...
2018: Bone Research
https://www.readbyqxmd.com/read/29507818/lrp1-in-osteoblasts-controls-osteoclast-activity-and-protects-against-osteoporosis-by-limiting-pdgf-rankl-signaling
#8
Alexander Bartelt, Friederike Behler-Janbeck, F Timo Beil, Till Koehne, Brigitte Müller, Tobias Schmidt, Markus Heine, Laura Ochs, Tayfun Yilmaz, Martin Dietrich, Jan P Tuckermann, Michael Amling, Joachim Herz, Thorsten Schinke, Joerg Heeren, Andreas Niemeier
Skeletal health relies on architectural integrity and sufficient bone mass, which are maintained through a tightly regulated equilibrium of bone resorption by osteoclasts and bone formation by osteoblasts. Genetic studies have linked the gene coding for low-density lipoprotein receptor-related protein1 (Lrp1) to bone traits but whether these associations are based on a causal molecular relationship is unknown. Here, we show that Lrp1 in osteoblasts is a novel regulator of osteoclast activity and bone mass. Mice lacking Lrp1 specifically in the osteoblast lineage displayed normal osteoblast function but severe osteoporosis due to highly increased osteoclast numbers and bone resorption...
2018: Bone Research
https://www.readbyqxmd.com/read/29507817/engineering-3d-approaches-to-model-the-dynamic-microenvironments-of-cancer-bone-metastasis
#9
REVIEW
Han Qiao, Tingting Tang
Cancer metastasis to bone is a three-dimensional (3D), multistep, dynamic process that requires the sequential involvement of three microenvironments, namely, the primary tumour microenvironment, the circulation microenvironment and the bone microenvironment. Engineered 3D approaches allow for a vivid recapitulation of in vivo cancerous microenvironments in vitro, in which the biological behaviours of cancer cells can be assessed under different metastatic conditions. Therefore, modelling bone metastasis microenvironments with 3D cultures is imperative for advancing cancer research and anti-cancer treatment strategies...
2018: Bone Research
https://www.readbyqxmd.com/read/29423331/transforming-growth-factor-%C3%AE-in-stem-cells-and-tissue-homeostasis
#10
Xin Xu, Liwei Zheng, Quan Yuan, Gehua Zhen, Janet L Crane, Xuedong Zhou, Xu Cao
TGF-β 1-3 are unique multi-functional growth factors that are only expressed in mammals, and mainly secreted and stored as a latent complex in the extracellular matrix (ECM). The biological functions of TGF-β in adults can only be delivered after ligand activation, mostly in response to environmental perturbations. Although involved in multiple biological and pathological processes of the human body, the exact roles of TGF-β in maintaining stem cells and tissue homeostasis have not been well-documented until recent advances, which delineate their functions in a given context...
2018: Bone Research
https://www.readbyqxmd.com/read/29423330/mtor-signaling-in-skeletal-development-and-disease
#11
Jianquan Chen, Fanxin Long
The mammalian/mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase that integrates inputs from nutrients and growth factors to control many fundamental cellular processes through two distinct protein complexes mTORC1 and mTORC2. Recent mouse genetic studies have established that mTOR pathways play important roles in regulating multiple aspects of skeletal development and homeostasis. In addition, mTORC1 has emerged as a common effector mediating the bone anabolic effect of Igf1, Wnt and Bmp...
2018: Bone Research
https://www.readbyqxmd.com/read/29354304/calcium-phosphate-cements-for-bone-engineering-and-their-biological-properties
#12
REVIEW
Hockin Hk Xu, Ping Wang, Lin Wang, Chongyun Bao, Qianming Chen, Michael D Weir, Laurence C Chow, Liang Zhao, Xuedong Zhou, Mark A Reynolds
Calcium phosphate cements (CPCs) are frequently used to repair bone defects. Since their discovery in the 1980s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. Much effort has been made to enhance the biological performance of CPCs, including their biocompatibility, osteoconductivity, osteoinductivity, biodegradability, bioactivity, and interactions with cells. This review article focuses on the major recent developments in CPCs, including 3D printing, injectability, stem cell delivery, growth factor and drug delivery, and pre-vascularization of CPC scaffolds via co-culture and tri-culture techniques to enhance angiogenesis and osteogenesis...
2017: Bone Research
https://www.readbyqxmd.com/read/29285402/bone-biomaterials-and-interactions-with-stem-cells
#13
REVIEW
Chengde Gao, Shuping Peng, Pei Feng, Cijun Shuai
Bone biomaterials play a vital role in bone repair by providing the necessary substrate for cell adhesion, proliferation, and differentiation and by modulating cell activity and function. In past decades, extensive efforts have been devoted to developing bone biomaterials with a focus on the following issues: (1) developing ideal biomaterials with a combination of suitable biological and mechanical properties; (2) constructing a cell microenvironment with pores ranging in size from nanoscale to submicro- and microscale; and (3) inducing the oriented differentiation of stem cells for artificial-to-biological transformation...
2017: Bone Research
https://www.readbyqxmd.com/read/29285401/multifunctional-stimuli-responsive-polymer-gated-iron-and-gold-embedded-silica-nano-golf-balls-nanoshuttles-for-targeted-on-demand-theranostics
#14
Liping Wang, Grace Jang, Deependra Kumar Ban, Vrinda Sant, Jay Seth, Sami Kazmi, Nirav Patel, Qingqing Yang, Joon Lee, Woraphong Janetanakit, Shanshan Wang, Brian P Head, Gennadi Glinsky, Ratneshwar Lal
Multi-functional nanoshuttles for remotely targeted and on-demand delivery of therapeutic molecules and imaging to defined tissues and organs hold great potentials in personalized medicine, including precise early diagnosis, efficient prevention and therapy without toxicity. Yet, in spite of 25 years of research, there are still no such shuttles available. To this end, we have designed magnetic and gold nanoparticles (NP)-embedded silica nanoshuttles (MGNSs) with nanopores on their surface. Fluorescently labeled Doxorubicin (DOX), a cancer drug, was loaded in the MGNSs as a payload...
2017: Bone Research
https://www.readbyqxmd.com/read/29263938/angiogenesis-in-a-3d-model-containing-adipose-tissue-stem-cells-and-endothelial-cells-is-mediated-by-canonical-wnt-signaling
#15
Xiaoxiao Cai, Jing Xie, Yang Yao, Xiangzhu Cun, Shiyu Lin, Taoran Tian, Bofeng Zhu, Yunfeng Lin
Adipose-derived stromal cells (ASCs) have gained great attention in regenerative medicine. Progress in our understanding of adult neovascularization further suggests the potential of ASCs in promoting vascular regeneration, although the specific cues that stimulate their angiogenic behavior remain controversial. In this study, we established a three-dimensional (3D) angiogenesis model by co-culturing ASCs and endothelial cells (ECs) in collagen gel and found that ASC-EC-instructed angiogenesis was regulated by the canonical Wnt pathway...
2017: Bone Research
https://www.readbyqxmd.com/read/29263937/experimental-repetitive-mild-traumatic-brain-injury-induces-deficits-in-trabecular-bone-microarchitecture-and-strength-in-mice
#16
Chandrasekhar Kesavan, Nikita M Bajwa, Heather Watt, Subburaman Mohan
To evaluate the long-term consequence of repetitive mild traumatic brain injury (mTBI) on bone, mTBI was induced in 10-week-old female C57BL/6J mice using a weight drop model, once per day for 4 consecutive days at different drop heights (0.5, 1 and 1.5 m) and the skeletal phenotype was evaluated at different time points after the impact. In vivo micro-CT (μ-CT) analysis of the tibial metaphysis at 2, 8 and 12 weeks after the impact revealed a 5%-32% reduction in trabecular bone mass. Histomorphometric analyses showed a reduced bone formation rate in the secondary spongiosa of 1...
2017: Bone Research
https://www.readbyqxmd.com/read/29263936/rankl-induced-m1-macrophages-are-involved-in-bone-formation
#17
Rong Huang, Xin Wang, Yinghong Zhou, Yin Xiao
The activation of M1 macrophages can be achieved by stimulating them with lipopolysaccharide (LPS) and interferon-γ (IFN-γ). However, M1 can be found under physiological conditions without any pathological stimuli. This study aimed to understand the involvement of RANKL-induced M1 macrophages in bone formation compared with pathologically induced macrophages. Fischer rats were used to investigate macrophage distribution in normal and injured femoral condyles in vivo . Bone marrow-derived macrophages (BMDMs) were activated with LPS+IFN-γ and RANKL to achieve M1 activation in vitro ...
2017: Bone Research
https://www.readbyqxmd.com/read/29167750/dysregulation-of-the-mir-146a-smad4-axis-impairs-osteogenesis-of-bone-mesenchymal-stem-cells-under-inflammation
#18
Wei Kuang, Liwei Zheng, Xin Xu, Yao Lin, Jiong Lin, Jiahua Wu, Jiali Tan
Osteoporosis is a common disease that affects patient quality of life, especially among the elderly population. Although inflammation contributes significantly to osteoporosis, the underlying mechanism is unclear. In this study, we found that tumor necrosis factor (TNF)-α, an inflammatory environment mimic, inhibits osteogenesis of bone mesenchymal stem cells (BMSCs), induces miR-146a and decreases Smad4. Moreover, overexpression of miR-146a inhibited the osteogenic ability of BMSCs, whereas blocking miR-146a partially rescued the osteogenesis deficiency under TNF - α treatment...
2017: Bone Research
https://www.readbyqxmd.com/read/29152381/selective-deletion-of-the-soluble-colony-stimulating-factor-1-isoform-in-vivo-prevents-estrogen-deficiency-bone-loss-in-mice
#19
Gang-Qing Yao, Nancy Troiano, Christine A Simpson, Karl L Insogna
Neutralizing CSF1 in vivo completely prevents ovariectomy (OVX)-induced bone loss in mice. There are two isoforms of CSF1, soluble (sCSF1), and membrane-bound (mCSF1), but their individual biological functions are unclear. It had been previously reported that mCSF1 knockout (K/O) and wild type (Wt) female mice experience the same degree of bone loss following OVX. In Wt mice the expression of sCSF1 was elevated fourfold in skeletal tissue following OVX while expression of mCSF1 was unchanged. To examine the role of sCSF1 in OVX-induced bone loss, mice were engineered in which sCSF1 was not expressed but expression of mCSF1 was unaffected (sCSF1 K/O)...
2017: Bone Research
https://www.readbyqxmd.com/read/29134132/abnormal-subchondral-bone-remodeling-and-its-association-with-articular-cartilage-degradation-in-knees-of-type-2-diabetes-patients
#20
Yan Chen, Yong-Can Huang, Chun Hoi Yan, Kwong Yuen Chiu, Qingjun Wei, Jingmin Zhao, X Edward Guo, Frankie Leung, William W Lu
Type 2 diabetes (T2D) is associated with systemic abnormal bone remodeling and bone loss. Meanwhile, abnormal subchondral bone remodeling induces cartilage degradation, resulting in osteoarthritis (OA). Accordingly, we investigated alterations in subchondral bone remodeling, microstructure and strength in knees from T2D patients and their association with cartilage degradation. Tibial plateaus were collected from knee OA patients undergoing total knee arthroplasty and divided into non-diabetic ( n =70) and diabetes ( n =51) groups...
2017: Bone Research
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