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Applications in Plant Sciences

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https://www.readbyqxmd.com/read/28224061/isolation-and-characterization-of-microsatellite-loci-in-sorbus-porrigentiformis-and-cross-amplification-in-s-aria-and-s-rupicola-rosaceae
#1
Rosalía Piñeiro, Freja Karrman-Bailey, Robyn S Cowan, Michael F Fay
PREMISE OF THE STUDY: Southwestern Britain is an emblematic hotspot of polyploid diversity of whitebeams (Sorbus aria agg.; Rosaceae) with ca. 30 polyploid endemic species. The tetraploid S. porrigentiformis is postulated as one of the parents of most of these endemics, along with the sexual diploid S. aria s. str. and the tetraploid S. rupicola. METHODS AND RESULTS: We isolated 16 nuclear microsatellite loci from S. porrigentiformis and characterized them on 45 trees representing the three putative parental species...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28224060/twenty-microsatellite-markers-for-the-endangered-vatica-mangachapoi-dipterocarpaceae
#2
Jun-Jie Guo, Shuai-Bin Shang, Chun-Sheng Wang, Zhi-Gang Zhao, Jie Zeng
PREMISE OF THE STUDY: Microsatellite markers were developed for Vatica mangachapoi (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China. METHODS AND RESULTS: Twenty microsatellite markers, including 12 polymorphic markers, were identified from V. mangachapoi using high-throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28224059/identification-of-downy-mildew-resistance-gene-candidates-by-positional-cloning-in-maize-zea-mays-subsp-mays-poaceae
#3
Jae Yoon Kim, Jun-Cheol Moon, Hyo Chul Kim, Seungho Shin, Kitae Song, Kyung-Hee Kim, Byung-Moo Lee
PREMISE OF THE STUDY: Positional cloning in combination with phenotyping is a general approach to identify disease-resistance gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combined strategy to improve the identification of disease-resistance gene candidates. METHODS AND RESULTS: Downy mildew (DM)-resistant maize was selected from five cultivars using a spreader row technique...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28224058/characterization-of-microsatellite-markers-in-two-exploited-african-trees-entandrophragma-candollei-and-e-utile-meliaceae
#4
Franck S Monthe, Jérôme Duminil, Félicien Tosso, Jérémy Migliore, Olivier J Hardy
PREMISE OF THE STUDY: Multiplexes of nuclear microsatellite primers were developed to investigate population genetic structure and diversity in two exploited African rainforest trees: Entandrophragma candollei and E. utile (Meliaceae). METHODS AND RESULTS: Microsatellite isolation was performed simultaneously on two nonenriched genomic libraries after next-generation sequencing. We developed 16 and 22 polymorphic markers for E. candollei and E. utile in three and four multiplexes, respectively...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28224057/identification-and-characterization-of-microsatellite-markers-in-pinus-kesiya-var-langbianensis-pinaceae
#5
Nian-Hui Cai, Yu-Lan Xu, Da-Wei Wang, Shi Chen, Gen-Qian Li
PREMISE OF THE STUDY: Microsatellite primers were developed in Pinus kesiya var. langbianensis (Pinaceae), a species native to southwestern China, to investigate its genetic diversity and population structure in order to provide information for the conservation and management of this species. METHODS AND RESULTS: Using next-generation sequencing, a total of 2349 putative simple sequence repeat primer pairs were designed. Eighteen polymorphic markers in 60 individuals belonging to four populations of P...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28224056/evaluation-and-comparison-of-fta-card-and-ctab-dna-extraction-methods-for-non-agricultural-taxa
#6
Chloe S Siegel, Florence O Stevenson, Elizabeth A Zimmer
PREMISE OF THE STUDY: An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS: DNA was extracted using FTA cards according to the manufacturer's protocol. In parallel, CTAB-based extractions were done using the automated AutoGen DNA isolation system...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28224055/is-biomass-a-reliable-estimate-of-plant-fitness
#7
Brett S Younginger, Dagmara Sirová, Mitchell B Cruzan, Daniel J Ballhorn
The measurement of fitness is critical to biological research. Although the determination of fitness for some organisms may be relatively straightforward under controlled conditions, it is often a difficult or nearly impossible task in nature. Plants are no exception. The potential for long-distance pollen dispersal, likelihood of multiple reproductive events per inflorescence, varying degrees of reproductive growth in perennials, and asexual reproduction all confound accurate fitness measurements. For these reasons, biomass is frequently used as a proxy for plant fitness...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090413/development-of-microsatellite-markers-in-garcinia-paucinervis-clusiaceae-an-endangered-species-of-karst-habitats
#8
Gang Hu, Zhong-Hua Zhang, Ping Yang, Qi-Wei Zhang, Chang-An Yuan
PREMISE OF THE STUDY: Microsatellite markers were developed for Garcinia paucinervis (Clusiaceae), an endangered and endemic tree species of karst habitats, to analyze its genetic diversity and genetic structure. METHODS AND RESULTS: Using shotgun sequencing on an Illumina MiSeq platform, a total of 22 microsatellite primer sets were characterized, of which 17 were identified as polymorphic. For these polymorphic loci, the total number of alleles per locus ranged from two to 12 across 54 individuals from three populations...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090412/development-characterization-and-cross-amplification-of-microsatellite-markers-in-the-understudied-african-genus-anthonotha-fabaceae
#9
Boris B Demenou, Olivier J Hardy
PREMISE OF THE STUDY: Anthonotha macrophylla (Fabaceae) is a common tree species throughout the Guineo-Congolian forest that is sometimes confounded with other congeneric species; it is expected to be an interesting phylogeographical model to infer the history of the African dense forests. We developed 18 microsatellite markers from this species and tested their transferability in 15 congeneric species. METHODS AND RESULTS: A genomic library was obtained using the Illumina platform, and 18 polymorphic microsatellite loci were developed...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090411/development-of-ssr-markers-by-454-sequencing-in-the-endemic-species-gentianella-praecox-subsp-bohemica-gentianaceae
#10
Mária Šurinová, Jiří Brabec, Zuzana Münzbergová
PREMISE OF THE STUDY: Polymorphic microsatellite loci were developed and used to genotype individuals of Gentianella praecox subsp. bohemica (Gentianaceae), a highly protected taxon in Europe, to study the genetic structure of the remaining populations. METHODS AND RESULTS: Thirty-eight primer pairs were successfully amplified; of these, 12 polymorphic microsatellite loci were developed using a 454 sequencing approach and used to genotype 180 individuals of G. praecox subsp...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090410/retrospective-analysis-of-heavy-metal-contamination-in-rhode-island-based-on-old-and-new-herbarium-specimens
#11
Sofia M Rudin, David W Murray, Timothy J S Whitfeld
PREMISE OF THE STUDY: Herbarium specimens may provide a record of past environmental conditions, including heavy metal pollution. To explore this potential, we compared concentrations of copper, lead, and zinc in historical and new collections from four sites in Rhode Island, USA. METHODS: We compared historical specimens (1846 to 1916) to congener specimens collected in 2015 at three former industrial sites in Providence, Rhode Island, and one nonindustrial site on Block Island...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090409/development-and-characterization-of-23-microsatellite-loci-for-rhododendron-ovatum-ericaceae
#12
De-Chen Liu, Yang Zhang, Si-Si Wang, Meng-Yu Liao, Xin-Yu Fan, Yuan-Yuan Li, Rong Wang
PREMISE OF THE STUDY: To estimate the genetic variation of Rhododendron ovatum (Ericaceae), a monoecious evergreen shrub, 23 microsatellite markers were identified from its nuclear genome. METHODS AND RESULTS: We developed 16 polymorphic and seven monomorphic microsatellite primers using the biotin-streptavidin capture method. The 16 polymorphic loci were investigated further using 89 individuals sampled from three populations in China. The number of alleles per locus ranged from four to 30, indicating a high level of polymorphism...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090408/development-of-microsatellite-markers-for-viscum-coloratum-santalaceae-and-their-application-to-wild-populations
#13
Bo-Yun Kim, Han-Sol Park, Soonok Kim, Young-Dong Kim
PREMISE OF THE STUDY: Microsatellite primers were developed for Viscum coloratum (Santalaceae), a semiparasitic medicinal plant that is known for its anticancer properties. Due to excessive human harvesting and loss of suitable habitat of its populations, it has become a potentially threatened species requiring immediate conservation efforts. METHODS AND RESULTS: Based on transcriptome data for V. coloratum, 124 primer pairs were randomly selected for initial validation, of which 19 yielded polymorphic microsatellite loci, with two to six alleles per locus...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090407/development-of-15-polymorphic-microsatellite-markers-for-ficus-virens-moraceae
#14
Rong-Hua Fu, Yun-Xiang Li, Mei Liu, Qiu-Mei Quan
PREMISE OF THE STUDY: Ficus virens (Moraceae) is distributed widely in South and Southeast Asia, Melanesia, and northern Australia, and it is also cultivated outside its original northern range limit in southwestern China. Therefore, the species is well suited to explore the mechanism of range limits of Ficus species. However, little is known about its genetic background. METHODS AND RESULTS: Fifteen polymorphic microsatellite markers were developed using the biotin-streptavidin capture method...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28090406/leaf-disc-grafting-for-the-transmission-of-candidatus-liberibacter-asiaticus-in-citrus-citrus-sinensis-rutaceae-seedlings
#15
Flavia Tabay Zambon, Karen Plant, Ed Etxeberria
PREMISE OF THE STUDY: The search for resistance/tolerance to the devastating citrus huanglongbing disease (syn. HLB or citrus greening) is generating an increasing number of new plants of diverse genetic makeup. As the increasing number of new plants require more space, resources, and time, the need for faster and more efficient HLB screening tests becomes crucial. METHODS AND RESULTS: The leaf-disc grafting system described here consists in replacing a disc of leaf tissue with a similar disc from an infected plant...
January 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28101439/development-of-highly-variable-microsatellite-markers-for-the-tetraploid-silene-stellata-caryophyllaceae
#16
Juannan Zhou, Michele R Dudash, Charles B Fenster, Elizabeth A Zimmer
PREMISE OF THE STUDY: We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success. METHODS AND RESULTS: A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di- or trinucleotide repeats...
December 2016: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28101438/development-and-characterization-of-est-ssr-markers-for-artocarpus-hypargyreus-moraceae
#17
Haijun Liu, Weizheng Tan, Hongbin Sun, Yu Liu, Kaikai Meng, Wenbo Liao
PREMISE OF THE STUDY: Polymorphic microsatellite markers were developed for Artocarpus hypargyreus (Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species. METHODS AND RESULTS: Based on the transcriptome data of A. hypargyreus, 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displayed clear polymorphisms across the 67 individuals from four populations of A...
December 2016: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28101437/characterization-of-16-microsatellite-markers-for-the-oreinotinus-clade-of-viburnum-adoxaceae
#18
Syndi Barish, Mónica Arakaki, Erika J Edwards, Michael J Donoghue, Wendy L Clement
PREMISE OF THE STUDY: Microsatellite loci were isolated from four species of Viburnum (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American Viburnum species of the Oreinotinus clade. METHODS AND RESULTS: Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of V. triphyllum and one population of V...
December 2016: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28101436/development-and-characterization-of-microsatellite-primers-in-the-endangered-mediterranean-shrub-ziziphus-lotus-rhamnaceae
#19
Ana González-Robles, Antonio J Manzaneda, Jesús M Bastida, Nick Harvey, Rafael Jaime, Teresa Salido, Luisa M Martínez, Ana Fernández-Ocaña, Julio M Alcántara, Pedro J Rey
PREMISE OF THE STUDY: Microsatellite primers were developed to characterize and evaluate patterns of genetic diversity and structure in the endangered Mediterranean shrub Ziziphus lotus (Rhamnaceae). METHODS AND RESULTS: Twenty microsatellite primers were developed for Z. lotus, of which 14 were polymorphic. We evaluated microsatellite polymorphism in 97 specimens from 18 Spanish and seven Moroccan populations. Between two and eight alleles were found per locus, and the average number of alleles was 5...
December 2016: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28101435/microsatellite-markers-for-nuphar-japonica-nymphaeaceae-an-aquatic-plant-in-the-agricultural-ecosystem-of-japan
#20
Toshiaki Kondo, Sonoko Watanabe, Takashi Shiga, Yuji Isagi
PREMISE OF THE STUDY: Nuphar species (Nymphaeaceae) are representative aquatic plants in irrigation ponds in Japanese agricultural ecosystems. We developed 15 polymorphic microsatellite markers for N. japonica and confirmed their utility for its close relatives N. oguraensis var. akiensis and N. ×saijoensis, which originated from natural hybridization between N. japonica and N. oguraensis. METHODS AND RESULTS: Genetic variation was characterized in 15 polymorphic loci in three populations of N...
December 2016: Applications in Plant Sciences
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