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Jundishapur Journal of Microbiology

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https://www.readbyqxmd.com/read/27942365/isolation-antimicrobial-susceptibility-profile-and-detection-of-sul1-blatem-and-blashv-in-amoxicillin-clavulanate-resistant-bacteria-isolated-from-retail-sausages-in-kampar-malaysia
#1
Lih-Shin Tew, Li-Yen She, Choy-Hoong Chew
BACKGROUND: Due to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern. OBJECTIVES: In this study, we aimed to detect the presence and discover the molecular determinants of foodborne bacteria in retail sausages resistant towards the antibacterial agent amoxicillin-clavulanate. METHODS: Two grams of sausages were chopped into small pieces and transferred into sterile Luria-Bertani (LB) enrichment broths overnight before they were plated on MacConkey agar petri dishes...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942364/a-low-prevalence-of-inducible-macrolide-lincosamide-and-streptogramin-b-resistance-phenotype-among-methicillin-susceptible-staphylococcus-aureus-isolated-from-malaysian-patients-and-healthy-individuals
#2
Asma Mesbah Elkammoshi, Hamed Ghasemzadeh-Moghaddam, Syafinaz Amin Nordin, Niazlin Mohd Taib, Suresh Kumar Subbiah, Vasanthakumari Neela, Rukman Awang Hamat
BACKGROUND: Antibiotic resistance among Staphylococcus aureus is of great concern worldwide. This resistance is further complicated by the ability of S. aureus to confer cross-resistance to other antibiotics due to the presence of resistance genes, such as erythromycin resistance methylase (erm) genes, which render the bacterium resistant to macrolide-lincosamide-streptogramin B (MLSB) antibiotics. Resistance to these antibiotics can lead to therapeutic failure, resulting in significant morbidity and mortality in patients with S...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942363/design-of-indigenous-elisa-using-tachyzoites-from-the-rh-strain-of-toxoplasma-gondii-and-comparison-with-commercial-kits-in-ahvaz-southwest-of-iran-2015
#3
Niloofar Mohammadpour, Jasem Saki, Abdollah Rafiei, Ali Khodadadi, Mehdi Tavalla, Bahman Cheraghian
BACKGROUND: Toxoplasma gondii is one of the most common causes of latent infections in humans worldwide. Detecting anti-Toxoplasma antibodies in serum using serological tests is a common method to diagnose toxoplasmosis. OBJECTIVES: In the present study, an indigenous ELISA kit was prepared using tachyzoites from the RH strain of T. gondii, and its sensitivity and specificity were compared with those of commercial kits. METHODS: To produce antigens, 0...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942362/immunization-with-protein-d-from-non-typeable-haemophilus-influenzae-nthi-induced-cytokine-responses-and-bioactive-antibody-production
#4
Atefeh Davoudi Vijeh Motlagh, Seyed Davar Siadat, Saeid Abedian Kenari, Mehdi Mahdavi, Ava Behrouzi, Hossein Asgarian-Omran
BACKGROUND: Outer membrane protein D (PD) is a highly conserved and stable protein in the outer membrane of both encapsulated (typeable) and non-capsulated (non-typeable) strains of Haemophilus influenzae. As an immunogen, PD is a potential candidate vaccine against non-typeable H. influenzae (NTHi) strains. OBJECTIVES: The aim of this study was to determine the cytokine pattern and the opsonic antibody response in a BALB/c mouse model versus PD from NTHi as a vaccine candidate...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942361/isolation-characterization-and-antibacterial-activity-of-bacteriophages-against-methicillin-resistant-staphylococcus-aureus-in-pakistan
#5
Muhammad Hidayat Rasool, Rukhsana Yousaf, Abu Baker Siddique, Muhammad Saqalein, Mohsin Khurshid
BACKGROUND: In recent years, antibiotic resistance has been indicated as a paramount threat to public health. The use of bacteriophages appears to be a safer alternative for the control of bacterial infections. OBJECTIVES: The present study aims to explore sewage water for the presence of indigenous bacteriophages, and to investigate their antibacterial potential against Methicillin-resistant Staphylococcus aureus (MRSA). METHODS: Bacterial isolates were first collected and identified from pus samples taken from the surgical and burn units using standard microbiological procedures...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942360/antibacterial-activity-of-silver-nanoparticles-and-their-combination-with-zataria-multiflora-essential-oil-and-methanol-extract
#6
Shirin Sheikholeslami, Seyyedeh Elaheh Mousavi, Hamid Reza Ahmadi Ashtiani, Seyed Reza Hosseini Doust, Seyed Mahdi Rezayat
BACKGROUND: Against a variety of antimicrobial resistant pathogens, the scientists attempted substitution of antimicrobial medicine with various nanoparticles and plant-based antibacterial substances. OBJECTIVES: The aim of this study was to assess the antibacterial effects of silver nanoparticles solely and in combination with Zataria multiflora essential oil and methanolic extract on some photogenic bacteria. METHODS: Minimum inhibitory concentrations (MICs) and fractional inhibitory concentrations (FICs) of plant essential oil, methanolic extract, and silver nanoparticles against bacteria were evaluated using the broth microdilution method and check board microtiter assays...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942359/cytolethal-distending-toxin-from-campylobacter-jejuni-requires-the-cytoskeleton-for-toxic-activity
#7
Estela T Méndez-Olvera, Jaime A Bustos-Martínez, Yolanda López-Vidal, Antonio Verdugo-Rodríguez, Daniel Martínez-Gómez
BACKGROUND: Campylobacter jejuni is one of the major causes of infectious diarrhea worldwide. The distending cytolethal toxin (CDT) of Campylobacter spp. interferes with normal cell cycle progression. This toxic effect is considered a result of DNase activity that produces chromosomal DNA damage. To perform this event, the toxin must be endocytosed and translocated to the nucleus. OBJECTIVES: The aim of this study was to evaluate the role of the cytoskeleton in the translocation of CDT to the nucleus...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942358/ndm-1-and-rmtc-producing-klebsiella-pneumoniae-isolates-in-turkey
#8
Tulin Guven Gokmen, Togrul Nagiyev, Melda Meral, Cansu Onlen, Farzad Heydari, Fatih Koksal
BACKGROUND: The resistance of aminoglycosides in strains that produce beta-lactamase can be developed through the multidrug resistant encoding genes carried by common plasmids. Recently, the association between 16S rRNA methyltransferase resistance and beta-lactamase enzymes carried by the same plasmids has drawn increased attention from researchers, particularly the association in aminoglycoside-resistant strains with a minimum inhibitory concentration (MIC) of ≥ 256 µg/mL. OBJECTIVES: We aimed to investigate the co-existence of 16S rRNA methyltransferase and beta-lactamase genes in multidrug resistant (MDR) Klebsiella pneumoniae strains isolated from clinical samples...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942357/the-effect-of-ferula-assa-foetida-l-and-carum-copticum-hydroalcoholic-extract-on-the-expression-levels-of-staphylococcus-aureus-genes-involved-in-quorum-sensing
#9
Najmeh Jomehpour, Gilda Eslami, Mohammad Bagher Khalili
BACKGROUND: Quorum sensing is a microbial cell-to-cell communication process. Quorum sensing bacteria produce and release extracellular messenger molecules called autoinducers. Gram-positive and Gram-negative, homoserine lactones, and oligopeptides are autoinducers used to communicate and regulate gene expression. OBJECTIVES: The goal of this study was to assess the impact of subinhibitory concentrations of Ferula assa-foetida l oleo-gum resin and Carum copticum fruit on the expression of tst and hld genes of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942356/rapid-detection-of-rifampicin-and-isoniazid-resistant-mycobacterium-tuberculosis-using-real-time-pcr
#10
Leyla Sahebi, Khalil Ansarin, Amir Monfaredan, Safar Farajnia, Seiran Nili, Majid Khalili
BACKGROUND: Accurate and rapid detection of drug-resistant Mycobacterium tuberculosis is fundamental for the successful treatment of tuberculosis (TB). OBJECTIVES: The aim of this study was to determine the frequency of common mutations leading to isoniazid (INH) and rifampicin (RMP) resistance. PATIENTS AND METHODS: In a cross-sectional study carried out in 2014, 90 patients with M. tuberculosis from five border provinces of Iran were selected...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942355/role-of-toll-like-receptors-in-tuberculosis-infection
#11
Oguz Oben Biyikli, Aysegul Baysak, Gulfem Ece, Adnan Tolga Oz, Mustafa Hikmet Ozhan, Afig Berdeli
BACKGROUND: One-third of the world's population is infected with Mycobacterium tuberculosis. Investigation of Toll-like receptors (TLRs) has revealed new information regarding the immunopathogenesis of this disease. Toll-like receptors can recognize various ligands with a lipoprotein structure in the bacilli. Toll-like receptor 2 and TLR-4 have been identified in association with tuberculosis infection. OBJECTIVES: The aim of our study was to investigate the relationship between TLR polymorphism and infection progress...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27942366/surveillance-of-antibiotic-resistant-bacteria-in-king-khalid-hospital-hafr-al-batin-saudi-arabia-during-2013
#12
Sulaiman Ali Al Yousef
BACKGROUND: Research to understand and control the emergence and spread of antimicrobial resistance has become a public health priority. OBJECTIVES: This study was conducted to study epidemiology and resistant pattern of bacteria causing infection in different King Khalid hospital units. PATIENTS AND METHODS: All samples were sent to the lab and routinely processed according to the standard microbiological procedures. Then, the cultures yielding growth were selected for the study...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800149/the-effects-of-sugars-on-the-biofilm-formation-of-escherichia-coli-185p-on-stainless-steel-and-polyethylene-terephthalate-surfaces-in-a-laboratory-model
#13
Mahdi Khangholi, Ailar Jamalli
BACKGROUND: Bacteria utilize various methods in order to live in protection from adverse environmental conditions. One such method involves biofilm formation; however, this formation is dependent on many factors. The type and concentration of substances such as sugars that are present in an environment can be effective facilitators of biofilm formation. METHODS: First, the physico-chemical properties of the bacteria and the target surface were studied via the MATS and contact angle measurement methods...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800148/prevalence-and-seasonal-distribution-of-respiratory-viruses-during-the-2014-2015-season-in-istanbul
#14
Safak Goktas, Mumtaz Cem Sirin
BACKGROUND: Acute respiratory tract infection (ARTI) is one of the most common infections worldwide, causing significant morbidity and mortality. OBJECTIVES: This study was conducted to determine the prevalence and seasonal distribution of respiratory viruses in our region, in children and adults with a pre-diagnosis of ARTI. METHODS: A total of 845 nasopharyngeal swab specimens were analyzed with the RespiFinder Smart 22 kit (PathoFinder BV, Netherlands) and the Rotor-Gene 6000 real-time PCR system...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800147/the-role-of-isocitrate-lyase-icl1-in-the-metabolic-adaptation-of-candida-albicans-biofilms
#15
Oluwaseun Ayodeji Ishola, Seng Yeat Ting, Yasser M Tabana, Mowaffaq Adam Ahmed, Muhammad Amir Yunus, Rafeezul Mohamed, Leslie Thian Lung Than, Doblin Sandai
BACKGROUND: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric substances, morphogenetic switching, and metabolic flexibility. OBJECTIVES: This study evaluated the roles of metabolic processes (in particular the glyoxylate cycle) on biofilm formation, antifungal drug resistance, morphology, and cell wall components...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800146/antimicrobial-activity-of-biocompatible-microemulsions-against-aspergillus-niger-and-herpes-simplex-virus-type-2
#16
Mayson H Alkhatib, Magda M Aly, Rajaa A Rahbeni, Khadijah S Balamash
BACKGROUND: Microemulsions (MEs), which consist of oil, water, surfactants, and cosurfactants, have recently generated considerable interest as antimicrobial agents. OBJECTIVES: To determine the antifungal and antiviral activities of three ME formulations (MEa, MEb, and MEc) that differ in their hydrophilicity. METHODS: The ME formulas were produced by mixing different fractions of Tween 80, Span 20, ethanol, oil, isopropyl myristate, and distilled water...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800145/molecular-diversity-of-mycobacterium-tuberculosis-strains-in-northwestern-iran
#17
Mahya Pourostadi, Jalil Rashedi, Behroz Mahdavi Poor, Hossein Samadi Kafil, Samaneh Shirazi, Mohammad Asgharzadeh
BACKGROUND: Years after the development of antituberculosis (TB) drugs, many people continue to suffer from this disease. To control the spread of TB, strains of the Mycobacterium tuberculosis complex need to be determined, and sources of infection must be identified. Such steps should help to prevent transmission of the infection. OBJECTIVES: The aim of this study was to perform molecular genotyping of isolates of the M. tuberculosis complex obtained from patients in northwestern Iran...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800144/seroprevalence-and-molecular-evaluation-of-toxoplasmosis-in-patients-undergoing-chemotherapy-for-malignancies-in-the-bushehr-province-southwest-iran
#18
Afshin Barazesh, Bahador Sarkari, Farhad Mehrabi Sisakht, Samaneh Abdolahi Khabisi, Reza Nikbakht, Mohammad Reza Ravanbod
BACKGROUND: Toxoplasmosis is a life-threatening infection in organ transplant recipients, people receiving corticosteroid or radiation therapy, people with malignancies, and AIDS patients. OBJECTIVES: The current study aimed to determine the prevalence of toxoplasmosis in patients receiving chemotherapy for malignancies in the Bushehr province of southwest Iran. METHODS: Blood samples were taken from 86 patients who were continuously referred to the chemotherapy center in Bushehr province and evaluated by ELISA to determine anti-Toxoplasma IgG and IgM antibodies...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800143/antimicrobial-activity-of-chitosan-films-with-essential-oils-against-listeria-monocytogenes-on-cabbage
#19
Gordana D Jovanovic, Anita S Klaus, Miomir P Niksic
BACKGROUND: The highest incidence of listeriosis, due to consumption of ready-to-eat foods and fresh, shredded, minimally processed vegetables, occurs among pregnant women and the elderly. In order to reduce the prevalence of listeriosis among consumers, better protective measures are recommended. Chitosan films, with or without added essential oils, represent a modern, safe method of preserving the quality of such vegetables and significantly reducing the incidence of Listeria monocytogenes in these foods...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27800142/antibacterial-efficacy-of-super-oxidized-water-on-enterococcus-faecalis-biofilms-in-root-canal
#20
Recai Zan, Tayfun Alacam, Ihsan Hubbezoglu, Tutku Tunc, Zeynep Sumer, Oguzhan Alici
BACKGROUND: The success of endodontic treatment depends on a few crucial factors. One of these factors is the complete chemomechanic preparation of root canal against various bacteria. In particular, the effect of resistant bacteria may cause intense pain with flare-up and formation of periapical lesions. Therefore, the strong effect of irrigants plays an important role in terms of the complete elimination of these bacteria to achieve long-term successful treatment. OBJECTIVES: The aim of this study was to investigate the antibacterial effects of super-oxidized water (SPO) in root canals infected with Enterococcus faecalis biofilms...
September 2016: Jundishapur Journal of Microbiology
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