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Asian-Australasian Journal of Animal Sciences

Zhijin Liu, Cunyuan Li, Xiaoyue Li, Yang Yao, Wei Ni, Xiangyu Zhang, Yang Cao, Wureli Hazi, Dawei Wang, Renzhe Quan, Shuting Yu, Yuyu Wu, Songmin Niu, Yulong Cui, Shengwei Hu
Objectve: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative Real-time PCR...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Renata Miltko, Małgorzata Paulina Majewska, Grzegorz Bełżecki, Katarzyna Kula, Barbara Kowalik
Objectve: The purpose of this study was to evaluate the effect of rapeseed and linseed oil supplementations on performance and meat quality of lambs. Methods: The experiment was conducted on 18 growing (100-day-old) lambs of 19.7 ± 1.9 kg live weight, assigned to 3 groups of 6 animals each. Control lambs were fed meadow hay and concentrate alone. Experimental animals additionally received rapeseed or linseed oils at a dose of 50 g/d. The lambs were slaughtered at an average body weight of 35...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Seong Shin Lee, Hyuk Jun Lee, Dimas Hand Vidya Paradhipta, Young Ho Joo, Sang Bum Kim, Dong Hyeon Kim, Sam Churl Kim
Objectve: This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure. Methods: Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3-5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (L. buchneri; LB) fermentative inoculants at 1.2 × 105 cfu/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a 2 x 2 (hybrid × inoculant) treatment arrangement for 100 days...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Jie Zhao, Zhihao Dong, Junfeng Li, Lei Chen, Yunfeng Bai, Yushan Jia, Tao Shao
Objectve: This study was to evaluate the fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro gas production of rice straw ensiled with lactic acid bacteria (LAB) and molasses. Methods: Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (C), Lactobacillus plantarum (L), molasses (M) and molasses + L. plantarum (ML) for 6, 15, 30 and 60 days. After storage, the silages were subjected to microbial and chemical analyses as well as the further in vitro fermentation trial...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Hyeok Joong Kang, Min Yu Piao, Seung Ju Park, Sang Weon Na, Hyun Jin Kim, Myunggi Baik
Objectve: This study was performed to evaluate whether ambient temperature and dietary rumen-protected fat (RPF) supplementation affect growth performance, rumen fermentation, and blood parameters in Korean cattle steers. Methods: Twenty Korean cattle steers [BW: 550.6 ± 9.14 kg, age: 19.7 ± 0.13 months] were divided into a conventional control diet group (n = 10) and a 0.5% RPF supplementation group (n = 10). Steers were fed a concentrate diet (1.6% BW) and a rice straw diet (1 kg/day) for 16 weeks [January 9 to February 5 (P1), February 6 to March 5 (P2), March 6 to April 3 (P3), and April 4 to May 2 (P4)]...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Hanbeen Kim, Eunsang Jung, Hyogun Lee, Byeongwoo Kim, Seongkeun Cho, Seyoung Lee, Inhyuk Kwon, Jakyeom Seo
Objectve: The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods: Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Lin Sun, Zhijun Wang, Ge Gentu, Yushan Jia, Meiling Hou, Yimin Cai
Objectve: To effectively use corn stover as animal feed, we studied the changes in microbial population and chemical composition of three cultivar corn stover during field exposure and their effects on silage fermentation and in vitro digestibility. Methods: The corn stover silages were prepared from stovers exposed in the field for 0, 7, 15, 30, 60, 90, and 180 d. The microbial composition, chemical composition, fermentation quality, and in vitro digestibility were analyzed...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Eduardo Felipe Colerauz de Oliveira Lazzarotto, Luís Fernando Glasenapp de Menezes, Wagner Paris, Marcos Luis Molinete, Gean Rodrigo Schmitz, José Henrique Ignacio Baviera, Roberta Farenzena, Adalberto Luiz de Paula
Objectves: The aim was to evaluate backgrounding beef steers on oat + ryegrass pastures mixed with vetch and/or using energy supplementation. Methods: A randomized block design with three treatments and three replications was used. The treatments were: grass+supplement (oat + ryegrass + supplementation), legume+supplement (oat + ryegrass + vetch + supplementation) and grass+legume (oat + ryegrass + vetch). A continuous grazing system with a variable stocking rate was used...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Xuezhi Ding, Chao Yang, Pengjia Bao, Xiaoyun Wu, Jie Pei, Ping Yan, Xian Guo
Objective: As an iconic symbol of Qinghai-Tibetan Plateau and of high altitude, yak is subjected to hypoxic conditions that challenge aerobic metabolism. Matrix metalloproteinases-3 (MMP3) is assumed to be a key target gene of hypoxia-inducible factor-1α (HIF-1α) that function as a master regulator of the cellular response to hypoxia. Therefore, the aim of this investigation was to identify the DNA polymorphism of MMP3 gene in domestic yak and to explore its possible association with high-altitude adaptation...
October 29, 2018: Asian-Australasian Journal of Animal Sciences
Guofeng Han, Hui Yang, Yunhao Wang, Shogo Haraguchi, Takuro Miyazaki, Takashi Bungo, Kosuke Tashiro, Mitsuhiro Furuse, Vishwajit S Chowdhury
Objective: Heat stress poses an increasing threat for poultry production. Some amino acids have been found to play critical roles in affording thermotolerance. Recently, it was found that in ovo administration of L-leucine (L-Leu) altered amino acid metabolism and afforded thermotolerance in heat-exposed broiler chicks. Methods: In this study, two doses (35 and 70 μmol/egg) of L-Leu were administered in ovo on embryonic day 7 to determine their effect on rectal temperature (RT), body weight (BW) and thyroid hormones at hatching...
October 29, 2018: Asian-Australasian Journal of Animal Sciences
Jakyeom Seo, Minho Song, Namchul Jo, Woonsu Kim, Sinyong Jeong, Jongnam Kim, Seyoung Lee, Seongwon Seo
Objective: This study was conducted to evaluate whether the co-injection of antioxidants together with foot-and-mouth disease (FMD) vaccination has the potential to attenuate the negative effects caused by vaccination in Holstein finishing steers. Methods: A total of 36 finishing Holstein steers (BW: 608 ± 45.6 kg, 17 months old) were randomly allocated to one of three treatments: 1) control (CON, only FMD vaccination without any co-injection), 2) co-injection of commercial non-steroidal anti-inflammatory drugs (NSAID) with FMD vaccination at a ratio of 10:1 (NSAID vol/ FMD vaccine vol) as a positive control (PCON), 3) co-injection of commercial mixture of vitamin E and selenium with FMD vaccination (VITESEL) (1 mL of FMD vaccine + 1 mL of antioxidants per 90 kg of BW)...
October 29, 2018: Asian-Australasian Journal of Animal Sciences
Miao Li, Shenfei Long, Qianqian Wang, Lianhua Zhang, Jiangxu Hu, Jie Yang, Zhibin Cheng, Xiangshu Piao
Objective: The objective of this study was to investigate effects of mixed organic acids (MOA) on nutrient digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high wheat bran diet. Methods: Six crossbred barrows (Duroc × Landrace × Yorkshire), with an average body weight 78.8 ± 4.21 kg, fitted with T-cannulas at the distal ileum, were allotted to a double 3 × 3 Latin square design with 3 periods and 3 diets...
October 29, 2018: Asian-Australasian Journal of Animal Sciences
Yeojin Hong, Anh Duc Truong, Janggeun Lee, Kyungbaek Lee, Geun-Bae Kim, Kang-Nyeong Heo, Hyun S Lillehoj, Yeong Ho Hong
Objective: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. Methods: Tissue samples were collected from 6-8-week-old Pekin duck (Anas platyrhynchos domesticus), total RNA was extracted and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, qRT-PCR was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity...
October 29, 2018: Asian-Australasian Journal of Animal Sciences
J S Yoo, K H Cho, J S Hong, H S Jang, Y H Chung, G T Kwon, D G Shin, Y Y Kim
Objective: This study was intended to investigate the nutrient ileal digestibility of dried mealworm (Tenebrio molitor) larvae and compare with those of three animal protein by-products in growing pigs. Methods: Dietary treatments included the following:1)Fish meal, corn-vegetable by-product basal diet + 9.95 % fish meal; 2)Meat meal, corn-vegetable by-product basal diet + 9.95 % meat meal; 3)Poultry meal, corn-vegetable by-product basal diet + 9.95 % poultry meal; 4) Tenebrio molitor, corn-vegetable by-product basal diet + 9...
October 29, 2018: Asian-Australasian Journal of Animal Sciences
Lubna Rafiq, Tahir Zahoor, Ambreen Sagheer, Nazia Khalid, Ubaid Ur Rahman, Atif Liaqat
Objective: The present work was undertaken to determine the possibility of using xanthan and guar gums as stabilizers to enhance the yogurt quality. Methods: Purposely, yogurt was manufactured from standardized milk (3.5% fat, 8.5% solid-not-fat contents) with the addition of 2-3% starter culture. Enzyme-hydrolyzed xanthan gum (0.1%, 0.5%, 1.0%) and guar gum (0.1%, 0.5%, 1.0%) were added to the yogurt as stabilizers. Prepared yogurt samples were kept at refrigeration temperature (4±2°C) for 21 days and various quality and sensory parameters were studied at regular intervals (7 days)...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
Siti Nadirah Ismail, Elmutaz Atta Awad, Idrus Zulkifli, Yong Meng Goh, Awis Qurni Sazili
Objective: The study was designed to investigate the effects of restraint method, restraint duration, and body weight on stress-linked hormones (corticosterone, adrenaline, and noradrenaline), blood biochemical (namely glucose and lactate), and the meat quality in broiler chickens. Methods: A total of 120 male broiler chickens (Cobb 500) were assigned to a 2 x 3 x 2 factorial arrangement in a completely randomized design using two restraint methods (shackle and cone), three durations of restraint (10, 30, and 60 s), and two categories of live body weight (1...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
Feng Xing, Chaoyang Zhang, Zhengquan Kong
Objective: Recent studies have demonstrated that LIN28B/let-7 plays a role in the regulation of pubertal onset in mammals. However, the role of LIN28B/ let-7 in the onset of ovine puberty remains unknown. We cloned the Duolang sheep Lin28B cDNA sequence, detected the expression change of LIN28B, let-7a and let-7g in hypothalamus, pituitary and ovary tissues at three different pubertal stages. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of LIN28B gene from Duolang sheep and the bioinformatics methods were applied to analyze the amino acid sequence of LIN28B protein...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
Shihei Touma, Hirotoshi Shimabukuro, Aisaku Arakawa, Takuro Oikawa
Objective: The Agu is the only native pig breed in Japan, which is reared in Okinawa prefecture, the southernmost region in Japan. Its origins are considered to be of Asian lineage; however, the genetic background of the Agu is still unclear. The objective of this study was to elucidate the maternal lineage of Okinawa indigenous Agu pig with the use of the mitochondrial DNA (mtDNA) control region. Methods: The mtDNA control regions of Agu pigs were sequenced and the phylogenetic relationship among Agu, East Asian and European pigs was investigated with the use of 78 Agu individuals...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
JiaYing Wang, Xiaolong Yuan, Shaopan Ye, Shuwen Huang, Yingting He, Hao Zhang, Jiaqi Li, Xiquan Zhang, Zhe Zhang
Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genome-wide association study (GWAS) using sequence data imputed from SNP panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K single nucleotide polymorphism (SNP) genotyping array...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
Thiet Nguyen, Somchai Chanpongsang, Narongsak Chaiyabutr, Sumpun Thammacharoen
Objective: The present study was carried out to evaluate the effect of high dietary cation and anion difference (DCAD) rations on diurnal variations in eating and meal patterns, water intake and urination patterns in dairy goats fed under high ambient temperature (HTa). Methods: Ten crossbred dairy goats during peri-parturition period were selected and divided into two groups of five animals each. Experimental diets were control DCAD (control, 22.8 mEq/100 g DM) and high DCAD (DCAD, 39...
October 26, 2018: Asian-Australasian Journal of Animal Sciences
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