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Asian-Australasian Journal of Animal Sciences

Janine Donaldson, Michael Taurai Madziva, Kennedy Honey Erlwanger
Objective: The current study aimed to investigate the impact of high-fat diets composed of different animal and vegetable fat sources on serum metabolic health markers in Japanese quail, as well as the overall lipid content and fatty acid profiles of the edible bird tissues following significantly increased dietary lipid supplementation. Methods: Fifty seven male quail were divided into six groups and fed either a standard diet or a diet enriched with one of five different fats (22 % coconut oil, lard, palm oil, soybean oil or sunflower oil) for 12 weeks...
October 20, 2016: Asian-Australasian Journal of Animal Sciences
Jae Gyu Yoo, Byeong-Woo Kim, Mi-Rung Park, Deug-Nam Kwon, Yun-Jung Choi, Teak-Soon Shin, Byung-Wook Cho, Jakyeom Seo, Jin-Hoi Kim, Seong-Keun Cho
Objective: The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts. Methods: Male and female fetal fibroblasts were transferred to in vitro-matured enucleated oocytes and in vitro and in vivo developmental competence of reconstructed embryos was investigated. And, a total of 6,789 female fibroblast nuclear-transferred embryos were surgically transferred into 41 surrogate gilts and 4,746 male fibroblast nuclear-transferred embryos were surgically transferred into 25 surrogate gilts...
October 20, 2016: Asian-Australasian Journal of Animal Sciences
Tae Sub Park, Si Won Kim, Jeong Hyo Lee
Objective: Transgenic technology is widely used for industrial applications and basic research. Systems that allow for genetic modification play a crucial role in biotechnology for a number of purposes, including the functional analysis of specific genes and the production of exogenous proteins. In this study, we examined and verified the cumate-inducible transgene expression system in chicken DF1 and quail QM7 cells, as well as loxP element-mediated transgene recombination using Cre recombinase in DF1 cells...
October 20, 2016: Asian-Australasian Journal of Animal Sciences
Wei Liu, Chang Hua Lin, Zheng Ke Wu, Guo Hua Liu, Hai Jie Yan, Hua Ming Yang, Hui Yi Cai
Objective: The net energy requirement for the maintenance (NEm) of broilers was determined using regression models by the indirect calorimetry method (ICM) or the comparative slaughter method (CSM). Methods: A 2×4 factorial arrangement of treatments including the evaluation method (ICM or CSM) and feed intake (25, 50, 75, or 100% of ad libitum recommended) was employed in this experiment. In the ICM, 96 male Arbor Acres (AA) birds aged d 15 were used with 4 birds per replicate and 6 replicates in each treatment...
October 19, 2016: Asian-Australasian Journal of Animal Sciences
Hwanhee Jang, Yohan Choi, Inkyu Yoo, Jisoo Han, Minjeong Kim, Hakhyun Ka
Objective: Prostaglandins (PGs) function in various reproductive processes, including luteolysis, maternal pregnancy recognition, conceptus development, and parturition. Our earlier study has shown that PG transporters ABCC4 and SLCO2A1 are expressed in the uterine endometrium in pigs. Since several other PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 are known to be present in the uterine endometrium, this study investigated the expression of these PG transporters in the porcine uterine endometrium and placenta...
October 19, 2016: Asian-Australasian Journal of Animal Sciences
Jeong Hyo Lee, Si Won Kim, Tae Sub Park
Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase...
October 19, 2016: Asian-Australasian Journal of Animal Sciences
Siran Wang, Xianjun Yuan, Zhihao Dong, Junfeng Li, Gang Guo, Yunfeng Bai, Junyu Zhang, Tao Shao
Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods: The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests...
October 19, 2016: Asian-Australasian Journal of Animal Sciences
Ruey-Chee Weng
The objective of this experiment was to evaluate the interplay of dietary lipid sources and feeding regime in the transition from sow milk to solid food of abruptly weaned piglets. 144 weaned piglets were trained over a 15 day period to experience gradually reducing dietary fat content from 12% to 6% for lard (L), soybean oil (S) and coconut oil (C) and their feeding behavior and diet preference then tested in a behavior observation experiment. Another 324 weaned piglets were used in three consecutive feeding experiments to measure the effect of different dietary fats on performance and feed choice in the four weeks after abrupt weaning...
October 5, 2016: Asian-Australasian Journal of Animal Sciences
Liang-Kun Lin, Fa-Jui Tan
The yield and quality of the broiler skin fat rendered through high and lower energy microwave rendering (3.6 W/g for 10 min and 2.4 W/g for 10 min for HEMR and LEMR, respectively), oven baking (OB, at 180 °C for 40 min), and water cooking (WC, boiling for 40 min) were compared. Microwave-rendered skin exhibited the highest yields and fat recovery rates, followed by OB, and WC fats (p<0.05). HEMR fat had the highest L*, a*, and b* values, whereas WC fat had the highest moisture content, acid values, and thiobarbituric acid (TBA) values (p<0...
October 5, 2016: Asian-Australasian Journal of Animal Sciences
Jeeyeon Lee, Eunji Gwak, Heeyoung Lee, Jimyeong Ha, Soomin Lee, Sejeong Kim, Mi-Hwa Oh, Beom-Young Park, Kyoung-Hee Choi, Yohan Yoon
Objective: The objective of this study was to evaluate the effect of combinations of NaNO2 and NaCl concentrations on Listeria monocytogenes growth in emulsion-type sausage. Methods: Sausages formulated with different combinations of NaNO2 (0 and 10 ppm) and NaCl (1.0%, 1.25% and 1.5%) were inoculated with a five-strain L. monocytogenes mixture, and stored at 4oC, 10oC or 15oC, under aerobic or vacuum conditions. L. monocytogenes cell counts were measured at appropriate intervals, and kinetic parameters such as growth rate and lag phase duration (LPD) were calculated using the modified Gompertz model...
October 5, 2016: Asian-Australasian Journal of Animal Sciences
Ashraf A Biswas, Sung Sill Lee, Lovelia Leal Mamuad, Seon Ho Kim, Yeon Jae Choi, Gui-Seck Bae, Kichoon Lee, Ha-Guyn Sung, Sang Suk Lee
This study was conducted to determine the effect of lysozyme addition on in vitro rumen fermentation and to identify the lysozyme inclusion rate for abating methane (CH4) production. An in vitro ruminal fermentation technique was done using a commercial concentrate to rice straw ratio of 8:2 as substrate. The following treatments were applied wherein lysozyme was added into 1 gm dry matter (DM) substrate at different levels of inclusion: without lysozyme, 2000, 4000, and 8000 U lysozyme. Results revealed that lysozyme addition had a significant effect on pH after 24 h of incubation with the highest pH (p<0...
September 28, 2016: Asian-Australasian Journal of Animal Sciences
Meiling Hou, Gentu Ge, Tingyu Liu, Yushan Jia, Yimin Cai
Silage preparation and fermentation quality of natural grasses in meadow steppe (MS) and typical steppe (TS) were studied. MS and TS contained thirty-three and nine species of natural grasses, respectively. Stipa Baicalensis in MS and Stipa grandi in TS were the dominant grasses with the highest dry matter (DM) yield. The crude protein (CP), neutral detergent fiber (NDF) and water-soluble carbohydrate (WSC) of the mixed natural grasses in both steppes were 8.02 to 9.03, 66.75 to 69.47 and 2.02 to 2.20% on a DM basis, respectively...
September 27, 2016: Asian-Australasian Journal of Animal Sciences
Hamid Reza Taheri, Kayvan Shirzadegan
A trial was conducted from 11 to 42 d post-hatch to investigate the effectiveness of the supplementation of a multiple-enzyme preparation (Natuzyme Plus) in a wheat-based diet on digesta viscosity, pH and microbial population, villus morphology, feed passage time, nutrient retention, carcass characteristics, blood lipid parameters and growth performance of broiler chickens. Three hundreds 10-d-old male Ross 308 chicks were allocated to three diets with five replicates of 20 birds per replicate. Dietary treatments were 1) a wheat-based diet (W), 2) W + Natuzyme Plus (WN; 500 mg/kg of the diet) and 3) a corn-based diet (C)...
September 27, 2016: Asian-Australasian Journal of Animal Sciences
Jianhui Li, Jianmin Yuan, Zhiqiang Miao, Yuming Guo
The objective of this experiment was to characterize the mRNA expression profile of type IIb sodium-inorganic phosphate cotransporter (NaPi-IIb) and the biochemical values of serum alkaline phosphatase (AKP), calcium, inorganic phosphorus, tibial ash and minerals of broiler chickens with aging. A total of 56 one-day-old Arbor Acres male broiler chickens were used. Broiler chickens were weighed and samples were collected weekly from day 1. The result showed that before the growth inflection point, ash, calcium, and phosphorus content in the tibia of broiler chickens increased with growth (before 3 weeks of age), although there were no significant differences in chicks at different ages in the later period of the experiment and weight gain rate was relatively slow at this stage (4 to 6 weeks)...
September 27, 2016: Asian-Australasian Journal of Animal Sciences
Guobin Luo, Wenbin Xu, Jinshan Yang, Yang Li, Liyang Zhang, Yizhen Wang, Cong Lin, Yonggen Zhang
This trial was performed to examine the effects of ruminally degradable starch levels in total mixed ration (TMR) with low corn-based starch on the milk production, whole-tract nutrient digestibility and nitrogen balance in dairy cows. Eight multiparous Holstein cows (BW: 717±63 kg; DIM: 169±29) were assigned to a crossover design with two dietary treatments: a diet containing 62.3% ruminally degradable starch (% of total starch, low RDS) or 72.1% ruminally degradable starch (% of total starch, high RDS)...
September 19, 2016: Asian-Australasian Journal of Animal Sciences
Saowalak Saree, Chaiyapoom Bunchasak, Choawit Rakangthong, Nuttawut Krutthai, Jessada Sakdee, Theerawit Poeikhampha
This experiment was conducted to evaluate the effects of a corn- or cassava- based diet on the production of meat-type ducks. Four hundred day-old ducks were used in this experiment. They were divided into five groups with each group replicated eight times. The ducks fed the corn-based diets served as the control group. The four other groups comprised different treatments, with each one given the cassava-based diet based on phase-feeding. Three treatments were fed the cassava-based diet from 16, 28 and 35 d; respectively up to 42 d of age and the other group was fed the cassava-based diet from 1 to 42 d of age...
September 19, 2016: Asian-Australasian Journal of Animal Sciences
Jinbiao Zhao, Zhongchao Li, Mingbin Lyu, Ling Liu, Xiangshu Piao, Defa Li
The objective of this study was to determine the effect of inclusion level on the digestible energy (DE), metabolizable energy (ME) and total tract digestibility of acid-hydrolyzed ether extract (AEE) for cottonseed oil when fed to growing pigs. Forty-two barrows (initial BW = 35.51 ± 2.01 kg) were randomly allotted to a completely randomized design with a corn-soybean meal basal diet, five levels of cottonseed oil (2%, 4%, 6%, 8% and 10%) and a 10% soybean oil diet. Each diet was replicated six times with one pig per replicate...
September 19, 2016: Asian-Australasian Journal of Animal Sciences
Young-Tae Heo, Woo-Tae Ha, Ran Lee, Won-Young Lee, Ha-Youn Jung, Kyu-Chan Hwang, Hyuk Song
Glucose is essential fuel in energy metabolism and synthesis pathways of all mammalian cells. In lactating animals, glucose is the major precursor for lactose and is a substrate for the synthesis of milk proteins and fat in mammary secretory (alveolar) epithelial cells. However, clear utilization of glucose in mammary cell during lactogenesis is still unknown due to lack of in vitro analyzing model. Therefore, objective of this study is to test reliability of mammary alveolar (MAC-T) cell as in vitro study model for glucose metabolism and lactating system...
September 19, 2016: Asian-Australasian Journal of Animal Sciences
M T Lee, W C Lin, B Yu, Tzu-Tai Lee
Oxidative stress suppresses animal health, performance and production, subsequently impact economic feasibility; hence, maintaining and improving oxidative status especially through natural nutrition strategy are essential for normal physiological process in animals. Phytochemicals are naturally occurring antioxidants that could be considered as one of the most promising material used in animal diet in various forms. In this review, their antioxidant effects on animals are discussed as reflected by improved apparent performance, productivity, and the internal physiological changes...
September 16, 2016: Asian-Australasian Journal of Animal Sciences
Jun Luo, Junying Liu, Hehe Liu, Tao Zhang, Jiwen Wang, Hua He, Chunchun Han
The bursa of Fabricius (BF) is a central humoral immune organ belonging to avian specifically. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Using Illumina next-generation sequencing, the data showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability...
September 16, 2016: Asian-Australasian Journal of Animal Sciences
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