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Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis

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https://www.readbyqxmd.com/read/25379012/mutagenesis-in-the-laci-gene-target-of-e-coli-improved-analysis-for-laci-d-and-laco-mutants
#1
Sarah J Swerdlow, Roel M Schaaper
The lacI gene of E. coli has been a highly useful target for studies of mutagenesis, particularly for analysis of the specificity (spectrum) of mutations generated under a variety of conditions and in various genetic backgrounds. The gene encodes the repressor of the lac operon, and lacI-defective mutants displaying constitutive expression of the operon are readily selected. DNA sequencing of the lacI mutants has often been confined to the N-terminal region of the protein, as it presents a conveniently short target with a high density of detectably mutable sites...
December 1, 2014: Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis
https://www.readbyqxmd.com/read/25147477/deletion-of-brca2-exon-27-causes-defects-in-response-to-both-stalled-and-collapsed-replication-forks
#2
Tae Moon Kim, Mi Young Son, Sherry Dodds, Lingchuan Hu, Paul Hasty
BRCA2 is a tumor suppressor that maintains genomic integrity through double strand break (DSB) repair and replication fork protection. The BRC motifs and an exon 27-encoded domain (Ex27) of BRCA2 interact with the recombinase RAD51 to respectively facilitate the formation and stability of a RAD51 filament on single strand DNA. The BRC-RAD51 associations enable DSB repair while the Ex27-RAD51 association protects the nascent replication strand from MRE11-mediated degradation. MRE11 is a nuclease that facilitates the generation of 3' overhangs needed for homologous recombination (HR)-mediated DSB repair...
August 2014: Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis
https://www.readbyqxmd.com/read/25083139/impact-of-abasic-site-orientation-within-nucleosomes-on-human-ape1-endonuclease-activity
#3
John M Hinz
Glycosylases responsible for recognizing DNA lesions and initiating Base Excision Repair (BER) are impeded by the presence of histones, which are essential for compaction of the genetic material in the nucleus. Abasic sites are an abundant mutagenic lesion in the DNA, arising spontaneously and as the product of glycosylase activity, making it a common intermediate in BER. The apurinic/apyrimidinic endonuclease 1 (APE1) recognizes abasic sites and cleaves the DNA backbone adjacent to the lesion, creating the single-strand break essential for the subsequent steps of BER...
August 2014: Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis
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