Eunnara Cho, Carol D Swartz, Andrew Williams, Miriam V Rivas, Leslie Recio, Kristine L Witt, Elizabeth K Schmidt, Jeffry Yaplee, Thomas H Smith, Phu Van, Fang Yin Lo, Charles C Valentine, Jesse J Salk, Francesco Marchetti, Stephanie L Smith-Roe, Carole L Yauk
Error-corrected duplex sequencing (DS) enables direct quantification of low-frequency mutations and offers tremendous potential for chemical mutagenicity assessment. We investigated the utility of DS to quantify induced mutation frequency (MF) and spectrum in human lymphoblastoid TK6 cells exposed to a prototypical DNA alkylating agent, N-ethyl-N-nitrosourea (ENU). Furthermore, we explored appropriate experimental parameters for this application, and assessed inter-laboratory reproducibility. In two independent experiments in two laboratories, TK6 cells were exposed to ENU (25-200 µM) and DNA was sequenced 48, 72, and 96 h post-exposure...
July 2023: Mutation Research. Genetic Toxicology and Environmental Mutagenesis