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https://www.readbyqxmd.com/read/29911157/molecular-evidence-and-phylogenetic-analysis-of-orf-virus-isolates-from-outbreaks-in-tripura-state-of-north-east-india
#1
Gnanavel Venkatesan, Ankan De, Sargam Arya, Amit Kumar, D Muthuchelvan, Bikas Ch Debnath, Tapan Kumar Dutta, Divakar Hemadri, A B Pandey
This study describes the first confirmed report of contagious ecthyma in Black Bengal goats from Tripura state, a North-Eastern state of India situated at the Indo-Bangladesh border. Outbreaks were characterized by the high rates of morbidity (58-67%), low mortality (8-10%) and case fatality (11-15%). The etiology of the outbreaks was confirmed as orf virus (ORFV) by standard virological/serological and molecular techniques including sequence analysis of B2L, a major envelop protein gene of genus Parapoxvirus ...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911156/complete-genome-sequencing-of-banana-bract-mosaic-virus-isolate-infecting-cardamom-revealed-its-closeness-to-banana-infecting-isolate-from-india
#2
A I Bhat, N S Pamitha, A Gopika, C N Biju
The complete genome of banana bract mosaic virus (BBrMV), a Potyvirus belonging to the family Potyviridae causing chlorotic streak disease of cardamom ( Elettaria cardamomum ) in India was determined for the first time from a naturally infected cardamom var. Njallani Green Gold through reverse transcription PCR using nine sets of primers designed to different overlapping regions of the genome. The complete genome has 9708 nucleotides excluding poly (A) tail and has the genome organization similar to that of BBrMV isolates infecting banana and flowering ginger ( Alpinia purpurata )...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911155/rna-based-vaccination-of-bhut-jolokia-pepper-capsicum-chinense-jacq-against-cucumber-mosaic-virus
#3
Munmi Borah, Margarita Berbati, Chrysavgi Reppa, Maria Holeva, Palash Deb Nath, Andreas Voloudakis
Cucumber mosaic virus (CMV) causes great losses in Bhut Jolokia pepper ( Capsicum chinense Jacq.) plantations in Assam, India. To investigate possible means to induce plant resistance against this virus, the crude extract of bacterially-expressed double-stranded (ds) RNA, derived from CMV-2b gene (dsRNA_CMV-2b), was exogenously applied along with CMV-G strain onto Bhut Jolokia plants. In this 'RNA-vaccination' bioassay, disease incidence, assessed by testing the plants at 21 days post inoculation by DAS-ELISA, ranged from 0 to 29% in case of dsRNA-treated plants, and from 55 to 92% when only CMV was applied...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911154/molecular-evidence-for-the-occurrence-of-tylcv-on-mentha-longifolia-in-jeddah-saudi-arabia
#4
Sayed Sartaj Sohrab, Ihsanullah Daur
Begomoviruses are whiteflies transmitted virus causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. Mentha is an important crop being grown here in Saudi Arabia for various purposes. The begomovirus associated disease was observed on Mentha crops during field survey which were growing near to tomato field. There is no published report available about the association of begomovirus on Mentha from this region. So, this work was conducted to identify the causative agent associated with yellow vein mosaic disease...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911153/comparison-of-a-rapid-immuno-chromatography-assay-with-a-standard-elisa-for-the-detection-of-igm-and-igg-antibodies-against-dengue-viruses
#5
Kalamathy Murugananthan, Pethirupillai A D Coonghe, Natkunam Ketheesan, Faseeha Noordeen
A total of 765 blood samples collected from dengue suspected patients admitted to a Teaching Hospital in Sri Lanka were used to compare a rapid ICT assay with a standard ELISA for the detection of anti-dengue virus (DENV) IgM and IgG. Detection accuracy indices including sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), Chi square and Cohen's kappa values were determined for the ICT assay using the ELISA as a comparator for the detection of anti-DENV IgM and IgG. The rapid ICT assay showed a sensitivity of 64%, specificity of 75%, NPV of 68% and PPV of 72% for anti-DENV IgM detection...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911152/development-of-a-rapid-test-for-detection-of-foot-and-mouth-disease-virus-specific-antibodies-using-gold-nanoparticles
#6
Beenu Jain, Upendra Lambe, Anuj Tewari, Surender Kumar Kadian, Minakshi Prasad
Foot and mouth disease is an economically important transboundary disease of wildlife and cloven hoofed animals including ruminants. In the absence of vaccination, detection of antibodies against structural proteins (SPs) of foot-and-mouth disease virus is an indicator of infection. In the present study, a rapid dot blot assay using gold nanoparticlees was developed for the detection of antibodies against SPs of FMDV. Commercially available FMD vaccine was used as a source of FMD antigen. After the synthesis of gold nanoparticles (GNPs), the GNP-dot blot assay was tested and was found very sensitive, as the detection of antibody was up to 10-4 of serum dilution...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911151/pathological-and-molecular-investigation-of-velogenic-viscerotropic-newcastle-disease-outbreak-in-a-vaccinated-chicken-flocks
#7
Asok Kumar Mariappan, Palanivelu Munusamy, Deepak Kumar, Shyma K Latheef, Shambu Dayal Singh, Rajendra Singh, Kuldeep Dhama
Newcastle disease virus (NDV) belongs to genus Avulavirus and family Paramyxoviridae. There are thirteen serotypes named APMV-I (Avian Paramyxovirus-I) to APMV-13 of which NDV has been designated as APMV-1. The disease has been reported worldwide affecting both domestic and wild avian species. Morbidity and mortality rates up to 100% have been reported in cases of unvaccinated flocks. Stringent vaccination schedule is practiced in endemic/disease prone areas in order to prevent the disease. Despite this, NDV outbreaks have been reported even in cases of vaccinated populations...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911150/cross-sectional-sero-prevalence-study-of-peste-des-petits-ruminants-ppr-in-goats-of-syangja-and-kaski-districts-of-nepal
#8
Narayan Acharya, Shankar Prasad Poudel, Krishna Prasad Acharya
A cross-sectional study was conducted to determine sero-prevalence of Peste des Petits Ruminants (PPR) among goat population of outbreak suspected areas; Kaski and Syangja districts of Nepal. A total of 460 goat serum samples were tested by using c-ELISA for the presence of antibodies against PPR. Out of the 460 samples tested, 380 samples were found positive, giving overall sero-positivity of 82.60%. Significantly higher sero-prevalence was found (* P  < 0.05) in females (87.50%) compared to males (70...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911149/characterization-of-marek-s-disease-virus-and-phylogenetic-analyses-of-meq-gene-from-an-outbreak-in-poultry-in-meghalaya-of-northeast-india
#9
Kekungu-U Puro, Uttaran Bhattacharjee, Samprity Baruah, Arnab Sen, Samir Das, Sandeep Ghatak, Sunil Doley, Rajkumari Sanjukta, Ingudam Shakuntala
The aim of the present study was to characterize the virus from the lesions and histopathology of organs associated with mortality in Kuroiler (dual purpose variety of poultry developed and marketed by Keggfarms Pvt. Ltd, India) birds suspected of Marek's disease. Among 1047 birds from two farms of different location with 5.5 and 34% mortality, two types of lesion were observed in post mortem examination; tumors in vital organs-liver, spleen, kidney, lung and ovaries and generalized small nodular tumour in the abdominal cavity...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911148/distribution-molecular-characterization-and-diversity-of-banana-bunchy-top-virus-in-tripura-india
#10
Tanmoy Das, Amrita Banerjee
Banana bunchy top virus (BBTV) is a serious threat to banana ( Musa spp.) production in India. Generally, BBTV isolates within the country share very low genetic diversity. However, in India, relatively greater diversity has been observed between isolates from north-eastern (NE) region (Meghalaya) and rest of India. Tripura is situated in the south-west corner of NE India and shares international border with Bangladesh. During 2014-2015, diagnostic surveys were conducted in seven districts of Tripura and polymerase chain reaction based detection established that BBTD is widely prevalent in all parts of Tripura showing an average incidence of 22...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911147/in-silico-targeting-of-non-structural-4b-protein-from-dengue-virus-4-with-spiropyrazolopyridone-study-of-molecular-dynamics-simulation-admet-and-virtual-screening
#11
Waqar Hussain, Iqra Qaddir, Sajid Mahmood, Nouman Rasool
Dengue fever is one of the most prevalent disease in tropical and sub-tropical regions of the world. According to the World Health Organisation (WHO), approximately 3.5 billion people have been affected with dengue fever. Four serotypes of dengue virus (DENV) i.e. DENV1, DENV2, DENV3 and DENV4 have up to 65% genetic variations among themselves. dengue virus 4 (DENV4) was first reported from Amazonas, Brazil and is spreading perilously due to lack of awareness of preventive measures, as it is the least targeted serotype...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911146/evaluation-of-dried-blood-spot-as-an-alternative-sample-collection-method-for-hepatitis-c-virus-rna-quantitation-and-genotyping-using-a-commercial-system
#12
Supriya Mahajan, Manish Chandra Choudhary, Guresh Kumar, Ekta Gupta
Dried blood spot (DBS) is a minimally invasive sampling method suitable for sample collection, storage and transportation in resource limited areas. Aim of this study was to compare the diagnostic utility of DBS with plasma sample for HCV RNA quantitation and genotyping using commercial systems. Plasma and DBS card spotted samples were collected from 95 HCV seropositive patients. Both types of samples were subjected to HCV RNA by real-time PCR (Abbott m2000rt, USA). Genotyping was performed using Abbott HCV genotype II kit (Abbott diagnostics, USA) in samples with viral load > 3 log10  IU/ml...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911145/mycovirus-associated-hypovirulence-a-potential-method-for-biological-control-of-fusarium-species
#13
REVIEW
Mohit Sharma, Shiwani Guleria, Kirti Singh, Anjali Chauhan, Saurabh Kulshrestha
Fusarium is a large genus of filamentous fungi belongs to the division Ascomycota and was first described as Fusisporium . Innumerable members of this genus act as pathogens, endophytes and saprophytes and can be recovered from plants and soils worldwide. Many of these members are known to be phytopathogens. It is among the most diverse and widely dispersed phyto-pathogenic fungi which cause economically important blights, rots, wilts and cankers of many ornamental, field, horticultural and forest crops both in agricultural commodities and natural ecosystems...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29911144/emergence-of-dengue-in-nepal
#14
REVIEW
Birendra Prasad Gupta, Mahesh Lamsal, Sudhikshya Chaulagain, Ramanuj Rauniyar, Rajani Malla, Smita Shrestha, Roshan Kurmi, Krishna Das Manandhar
Dengue virus is a major health problem in Nepal. The endogenous dengue appeared in 2006 in the country with reported outbreaks in 2010, 2013 and 2016. Eleven years vertical data show there were sporadic cases in all the years and mostly adults between 25 and 40 years of age were infected with dengue virus. Compared with primary infections, secondary infections were observed in relatively larger numbers during the period of 2008-2016. Most of the cases had symptoms of dengue fever; while 7 and 19 cases demonstrated dengue hemorrhagic fever/dengue shock syndrome in 2010 and 2013 respectively...
June 2018: Virusdisease
https://www.readbyqxmd.com/read/29608205/correction-to-efficacy-of-h120-and-ma5-avian-infectious-bronchitis-vaccines-in-early-challenge-against-qx-strain
#15
Vahid Karimi, Arash Ghalyanchilangeroudi, Masoud Hashemzadeh, Forough Rahimi, Mohammad Taha Zabihi Petroudi, Reza Kh Farahani, Hossein Maghsoudloo, Hamed Abdollahi
[This corrects the article DOI: 10.1007/s13337-017-0414-4.].
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29608185/correction-to-lack-of-relationship-between-pten-32-bp-and-tp53-16-bp-ins-del-polymorphisms-and-chronic-hepatitis-b-virus-infection
#16
Ebrahim Eskandari, Tayebeh Dahmardeh, Fatemeh Dahmardeh, Elham Pahlevani, Malihe Metanat
[This corrects the article DOI: 10.1007/s13337-017-0391-7.].
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29607371/efficacy-of-h120-and-ma5-avian-infectious-bronchitis-vaccines-in-early-challenge-against-qx-strain
#17
Vahid Karimi, Arash Ghalyanchilangeroudi, Masoud Hashemzadeh, Forough Rahimi, Mohammad Taha Zabihi Petroudi, Reza Kh Farahani, Hossein Maghsoudloo, Hamed Abdollahi
Infectious bronchitis (IB) is a highly infectious avian pathogen, which affects the respiratory tract, gut, reproductive system, and kidney of chicks of all ages. Many different serotypes of IB virus (IBV) are recognized which cause different clinical manifestations. According to the antigenic differences, different serotypes of the virus do not cross-protect. Massachusetts serotype induces the best cross-protection against other serotypes. Recently, the IBV QX strain has been detected in Iran. QX strain causes permanent damage to the oviduct if it occurs in the early life cycle and is a significant factor in layer and breeder chicken flocks...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29607370/the-evidence-of-occurrence-of-porcine-circovirus-2-isolation-and-characterization-in-kazakhstan
#18
M Mambetaliyev, N B Yesimbekova, V M Strochkov, K K Tabynov, K T Sultankulova, Ye O Abduraimov
This report describes the first isolation and characterization of porcine circovirus 2 (PCV2) in the Republic of Kazakhstan. The virus was isolated from a dead piglet that did not exhibit any typical clinical symptoms of porcine circovirus disease at a pig factory in North Kazakhstan oblast (region). The isolated virus belongs to genotype 2 (PCV2) and shares 96.6% sequence homology with one isolate and two strains from China and two French strains in group 1, cluster 1ab and 96.4% homology with two strains isolated in China and one strain from Hungary...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29607369/molecular-epidemiological-survey-of-canine-parvovirus-in-domestic-dogs-in-four-provinces-china
#19
Hongchao Wu, Xiangdong Li, Lingxiao Wang, Yuxiu Liu, Kegong Tian
Canine parvovirus (CPV) can cause severe disease in animals, especially in dogs, and continuously generates new variants. In this study, the complete VP2 genes of 59 CPV isolates from clinical diseased dogs between 2015 and 2016 in 4 Chinese provinces were sequenced and analyzed. The results showed that new CPV-2a was still the prominent CPV genotype, followed by CPV-2c and new CPV-2b. CPV-2c with Ala5Gly and Gln370Arg mutations was first detected in Henan, Guangxi and Jiangsu provinces in China. A phylogenetic tree based on VP2 sequences showed that all isolates in this study formed a close lineage which separated from foreign isolates...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29607368/canine-parvovirus-types-2a-and-2c-detection-from-dogs-with-suspected-parvoviral-enteritis-in-peru
#20
Raquel Quino Quispe, Luis Luna Espinoza, Rocío Rímac Beltrán, Raúl Rosadio Alcántara, Lenin Maturrano Hernández
Canine parvovirus type 2 (CPV-2) has been reported worldwide as the main agent related to acute hemorrhagic enteritis of high morbidity and variable mortality in puppies. The detection and characterization of this virus is essential to understand the etiology of the disease and to develop control measures. To characterize the virus circulating in Peruvian dogs and to provide new insights into the local diversity of CPV-2, rectal swabs from 39 puppies with clinical symptoms and with no history of previous vaccinations were analyzed...
March 2018: Virusdisease
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