journal
MENU ▼
Read by QxMD icon Read
search

Biosensors

journal
https://www.readbyqxmd.com/read/29149074/central-cns-and-peripheral-gastric-tissue-selective-monitoring-of-somatostatin-srif-with-micro-sensor-and-voltammetry-in-rats-influence-of-growth-factors-gh-egf
#1
Francesco Crespi
Somatostatin (SRIF) is widely distributed throughout the body, and regulates the endocrine system via interactions with various hormones, including the pituitary growth hormone, the thyroid stimulating hormone and the majority of the hormones of the gastrointestinal tract. SRIF is present in the central nervous system (CNS), where it affects rates of neurotransmission, and is also reported to be active in the intestinal tract, with evidence that stressed rats present a significant decrease in antral somatostatin-like immunoreactivity (SLI)...
November 17, 2017: Biosensors
https://www.readbyqxmd.com/read/29140259/ramanomics-new-omics-disciplines-using-micro-raman-spectrometry-with-biomolecular-component-analysis-for-molecular-profiling-of-biological-structures
#2
REVIEW
Andrey N Kuzmin, Artem Pliss, Paras N Prasad
Modern instrumentation for Raman microspectroscopy and current techniques in analysis of spectral data provide new opportunities to study molecular interactions and dynamics at subcellular levels in biological systems. Implementation of biomolecular component analysis (BCA) to microRaman spectrometry provides basis for the emergence of Ramanomics, a new biosensing discipline with unprecedented capabilities to measure concentrations of distinct biomolecular groups in live cells and organelles. Here we review the combined use of microRaman-BCA techniques to probe absolute concentrations of proteins, DNA, RNA and lipids in single organelles of live cells...
November 15, 2017: Biosensors
https://www.readbyqxmd.com/read/29137201/review-on-sers-of-bacteria
#3
REVIEW
Pamela A Mosier-Boss
Surface enhanced Raman spectroscopy (SERS) has been widely used for chemical detection. Moreover, the inherent richness of the spectral data has made SERS attractive for use in detecting biological materials, including bacteria. This review discusses methods that have been used to obtain SERS spectra of bacteria. The kinds of SERS substrates employed to obtain SERS spectra are discussed as well as how bacteria interact with silver and gold nanoparticles. The roll of capping agents on Ag/Au NPs in obtaining SERS spectra is examined as well as the interpretation of the spectral data...
November 13, 2017: Biosensors
https://www.readbyqxmd.com/read/29137135/an-electrochemical-enzyme-biosensor-for-3-hydroxybutyrate-detection-using-screen-printed-electrodes-modified-by-reduced-graphene-oxide-and-thionine
#4
Gonzalo Martínez-García, Elena Pérez-Julián, Lourdes Agüí, Naomí Cabré, Jorge Joven, Paloma Yáñez-Sedeño, José Manuel Pingarrón
A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD⁺ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0...
November 11, 2017: Biosensors
https://www.readbyqxmd.com/read/29088096/strategies-using-bio-layer-interferometry-biosensor-technology-for-vaccine-research-and-development
#5
REVIEW
Rejane L Petersen
Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer high-throughput, ease of use, reliability, and high precision analysis when compared with common labeling techniques. Many different strategies have been used to immobilize the pathogen or host molecules on BLI biosensors for real-time kinetics and affinity analysis, quantification, or high-throughput titer. These strategies can be used in multiple applications and shed light onto the structural and functional aspects molecules play during pathogen-host interactions...
October 31, 2017: Biosensors
https://www.readbyqxmd.com/read/29057816/encapsulation-stabilized-europium-containing-nanoparticle-as-a-probe-for-time-resolved-luminescence-detection-of-cardiac-troponin-i
#6
Ka Ram Kim, Yong Duk Han, Hyeong Jin Chun, Kyung Won Lee, Dong-Ki Hong, Kook-Nyung Lee, Hyun C Yoon
The use of a robust optical signaling probe with a high signal-to-noise ratio is important in the development of immunoassays. Lanthanide chelates are a promising material for this purpose, which provide time-resolved luminescence (TRL) due to their large Stokes shift and long luminescence lifetime. From this, they have attracted considerable interest in the in vitro diagnostics field. However, the direct use of lanthanide chelates is limited because their luminescent signal can be easily affected by various quenchers...
October 18, 2017: Biosensors
https://www.readbyqxmd.com/read/29036932/electrospun-chitosan-gelatin-biopolymer-composite-nanofibers-for-horseradish-peroxidase-immobilization-in-a-hydrogen-peroxide-biosensor
#7
Siriwan Teepoo, Phanphruk Dawan, Naris Barnthip
A biosensor based on chitosan-gelatin composite biopolymers nanofibers is found to be effective for the immobilization of horseradish peroxidase to detect hydrogen peroxide. The biopolymer nanofibers were fabricated by an electrospining technique. Upon optimization of synthesis parameters, biopolymers nanofibers, an average of 80 nm in diameter, were obtained and were then modified on the working electrode surface. The effects of the concentration of enzyme, pH, and concentration of the buffer and the working potential on the current response of the nanofibers-modified electrode toward hydrogen peroxide were optimized to obtain the maximal current response...
October 15, 2017: Biosensors
https://www.readbyqxmd.com/read/29027976/development-and-bioanalytical-applications-of-a-white-light-reflectance-spectroscopy-label-free-sensing-platform
#8
REVIEW
Georgios Koukouvinos, Panagiota Petrou, Dimitrios Goustouridis, Konstantinos Misiakos, Sotirios Kakabakos, Ioannis Raptis
The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determination of either high (e.g., proteins) or low molecular weight analytes (e.g., pesticides), is described. At the same time, the passage from single to multi-analyte determinations, and from a laboratory prototype set-up to a compact device appropriate for on-site determination, is outlined...
October 13, 2017: Biosensors
https://www.readbyqxmd.com/read/28972568/cfd-modeling-of-chamber-filling-in-a-micro-biosensor-for-protein-detection
#9
Meiirbek Islamov, Marzhan Sypabekova, Damira Kanayeva, Luis Rojas-Solórzano
Tuberculosis (TB) remains one of the main causes of human death around the globe. The mortality rate for patients infected with active TB goes beyond 50% when not diagnosed. Rapid and accurate diagnostics coupled with further prompt treatment of the disease is the cornerstone for controlling TB outbreaks. To reduce this burden, the existing gap between detection and treatment must be addressed, and dedicated diagnostic tools such as biosensors should be developed. A biosensor is a sensing micro-device that consists of a biological sensing element and a transducer part to produce signals in proportion to quantitative information about the binding event...
October 3, 2017: Biosensors
https://www.readbyqxmd.com/read/28974001/comparison-of-sensitivity-and-quantitation-between-microbead-dielectrophoresis-based-dna-detection-and-real-time-pcr
#10
Michihiko Nakano, Zhenhao Ding, Junya Suehiro
In this study, we describe a microbead-based method using dielectrophoresis (DEP) for the fast detection of DNA amplified by polymerase chain reaction (PCR). This electrical method measures the change in impedance caused by DEP-trapped microbeads to which biotinylated target DNA molecules are chemically attached. Using this method, measurements can be obtained within 20 min. Currently, real-time PCR is among the most sensitive methods available for the detection of target DNA, and is often used in the diagnosis of infectious diseases...
September 30, 2017: Biosensors
https://www.readbyqxmd.com/read/28956820/raman-computational-and-experimental-studies-of-dopamine-detection
#11
John D Ciubuc, Kevin E Bennet, Chao Qiu, Matthew Alonzo, William G Durrer, Felicia S Manciu
A combined theoretical and experimental analysis of dopamine (DA) is presented in this work with the objective of achieving more accurate detection and monitoring of this neurotransmitter at very low concentrations, specific to physiological levels. Surface-enhanced Raman spectroscopy on silver nanoparticles was employed for recording DA concentrations as low as 10(-11) molar. Quantum chemical density functional calculations were carried out using Gaussian-09 analytical suite software. Relatively good agreement between the simulated and experimentally determined results indicates the presence of different DA molecular forms, such as uncharged DA(±), anionic DA(-), and dopaminequinone...
September 28, 2017: Biosensors
https://www.readbyqxmd.com/read/28946617/a-review-on-passive-and-integrated-near-field-microwave-biosensors
#12
REVIEW
Subhajit Guha, Farabi Ibne Jamal, Christian Wenger
In this paper we review the advancement of passive and integrated microwave biosensors. The interaction of microwave with biological material is discussed in this paper. Passive microwave biosensors are microwave structures, which are fabricated on a substrate and are used for sensing biological materials. On the other hand, integrated biosensors are microwave structures fabricated in standard semiconductor technology platform (CMOS or BiCMOS). The CMOS or BiCMOS sensor technology offers a more compact sensing approach which has the potential in the future for point of care testing systems...
September 23, 2017: Biosensors
https://www.readbyqxmd.com/read/28934106/biosensor-technology-reveals-the-disruption-of-the-endothelial-barrier-function-and-the-subsequent-death-of-blood-brain-barrier-endothelial-cells-to-sodium-azide-and-its-gaseous-products
#13
Dan T Kho, Rebecca H Johnson, Simon J O'Carroll, Catherine E Angel, E Scott Graham
Herein we demonstrate the sensitive nature of human blood-brain barrier (BBB) endothelial cells to sodium azide and its gaseous product. Sodium azide is known to be acutely cytotoxic at low millimolar concentrations, hence its use as a biological preservative (e.g., in antibodies). Loss of barrier integrity was noticed in experiments using Electric Cell-substrate Impedance Sensing (ECIS) biosensor technology, to measure endothelial barrier integrity continuously in real-time. Initially the effect of sodium azide was observed as an artefact where it was present in antibodies being employed in neutralisation experiments...
September 21, 2017: Biosensors
https://www.readbyqxmd.com/read/28930184/use-of-wavelet-transform-to-detect-compensated-and-decompensated-stages-in-the-congestive-heart-failure-patient
#14
Pratibha Sharma, Kimberly Newman, Carlin S Long, A J Gasiewski, Frank Barnes
This research work is aimed at improving health care, reducing cost, and the occurrence of emergency hospitalization in patients with Congestive Heart Failure (CHF) by analyzing heart and lung sounds to distinguish between the compensated and decompensated states. Compensated state defines stable state of the patient but with lack of retention of fluids in lungs, whereas decompensated state leads to unstable state of the patient with lots of fluid retention in the lungs, where the patient needs medication. Acoustic signals from the heart and the lung were analyzed using wavelet transforms to measure changes in the CHF patient's status from the decompensated to compensated and vice versa...
September 20, 2017: Biosensors
https://www.readbyqxmd.com/read/28930183/substituting-sodium-hydrosulfite-with-sodium-metabisulfite-improves-long-term-stability-of-a-distributable-paper-based-test-kit-for-point-of-care-screening-for-sickle-cell-anemia
#15
Kian Torabian, Dalia Lezzar, Nathaniel Z Piety, Alex George, Sergey S Shevkoplyas
Sickle cell anemia (SCA) is a genetic blood disorder that is particularly lethal in early childhood. Universal newborn screening programs and subsequent early treatment are known to drastically reduce under-five SCA mortality. However, in resource-limited settings, cost and infrastructure constraints limit the effectiveness of laboratory-based SCA screening programs. To address this limitation our laboratory previously developed a low-cost, equipment-free, point-of-care, paper-based SCA test. Here, we improved the stability and performance of the test by replacing sodium hydrosulfite (HS), a key reducing agent in the hemoglobin solubility buffer which is not stable in aqueous solutions, with sodium metabisulfite (MS)...
September 20, 2017: Biosensors
https://www.readbyqxmd.com/read/28927005/mediatorless-impedance-studies-with-titanium-dioxide-conjugated-gold-nanoparticles-for-hydrogen-peroxide-detection
#16
Nur Hamidah Abdul Halim, Yook Heng Lee, Radha Swathe Priya Malon Marugan, Uda Hashim
An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO₂) particles for hydrogen peroxide (H₂O₂) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO₂, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-like activity, was used as the bioreceptor in the biosensor to detect H₂O₂ in solution...
September 18, 2017: Biosensors
https://www.readbyqxmd.com/read/28906469/silicon-integrated-dual-mode-interferometer-with-differential-outputs
#17
Niklas Hoppe, Pascal Scheck, Rami Sweidan, Philipp Diersing, Lotte Rathgeber, Wolfgang Vogel, Benjamin Riegger, Alexander Southan, Manfred Berroth
The dual-mode interferometer (DMI) is an attractive alternative to Mach-Zehnder interferometers for sensor purposes, achieving sensitivities to refractive index changes close to state-of-the-art. Modern designs on silicon-on-insulator (SOI) platforms offer thermally stable and compact devices with insertion losses of less than 1 dB and high extinction ratios. Compact arrays of multiple DMIs in parallel are easy to fabricate due to the simple structure of the DMI. In this work, the principle of operation of an integrated DMI with differential outputs is presented which allows the unambiguous phase shift detection with a single wavelength measurement, rather than using a wavelength sweep and evaluating the optical output power spectrum...
September 14, 2017: Biosensors
https://www.readbyqxmd.com/read/28878194/a-rationally-designed-reversible-turn-off-sensor-for-glutathione
#18
Sabrina Heng, Xiaozhou Zhang, Jinxin Pei, Andrew D Abell
γ-Glutamyl-cysteinyl-glycine (GSH) plays a critical role in maintaining redox homeostasis in biological systems and a decrease in its cellular levels is associated with diseases. Existing fluorescence-based chemosensors for GSH acts as irreversible reaction-based probes that exhibit a maximum fluorescence ('turn-on') once the reaction is complete, regardless of the actual concentration of GSH. A reversible, reaction-based 'turn-off' probe ( 1 ) is reported here to sense the decreasing levels of GSH, a situation known to occur at the onset of various diseases...
September 6, 2017: Biosensors
https://www.readbyqxmd.com/read/28858219/a-novel-impedance-biosensor-for-measurement-of-trans-epithelial-resistance-in-cells-cultured-on-nanofiber-scaffolds
#19
Robert A Schramm, Matthew H Koslow, Deirdre A Nelson, Melinda Larsen, James Castracane
Nanofibrous scaffolds provide high surface area for cell attachment, and resemble the structure of the collagen fibers which naturally occur in the basement membrane and extracellular matrix. A label free and non-destructive method of assessing the interaction of cell tissue and scaffolds aids in the ability to discern the effective quality and magnitude of any scaffold modifications. Impedance cell spectroscopy is a biosensing method that employs a functional approach to assessing the cell monolayer. The electrical impedance barrier function of a cell monolayer represents the level of restriction to diffusion of charged species between all adjacent cells across an entire contiguous cellular monolayer...
August 31, 2017: Biosensors
https://www.readbyqxmd.com/read/28820461/thermal-response-analysis-of-phospholipid-bilayers-using-ellipsometric-techniques
#20
Carmen M González-Henríquez, Vanessa A Villegas-Opazo, Dallits H Sagredo-Oyarce, Mauricio A Sarabia-Vallejos, Claudio A Terraza
Biomimetic planar artificial membranes have been widely studied due to their multiple applications in several research fields. Their humectation and thermal response are crucial for reaching stability; these characteristics are related to the molecular organization inside the bilayer, which is affected by the aliphatic chain length, saturations, and molecule polarity, among others. Bilayer stability becomes a fundamental factor when technological devices are developed-like biosensors-based on those systems...
August 18, 2017: Biosensors
journal
journal
48211
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"