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Gerardo Grasso, Ludovico Caracciolo, Giulia Cocco, Chiara Frazzoli, Roberto Dragone
Simazine is an herbicide that is able to contaminate surface waters, ground waters, and milk/dairy products, thus posing concerns in both environmental health and food safety. A yeast-based bioprobe was utilized to detect simazine in spiked real samples of livestock drinking water and raw cow's milk. Yeast aerobic respiration was taken as short-term toxicological endpoint. We carried out comparative measures of yeast oxygen consumption between simazine-spiked samples and blank samples. Percentage interference (%ρ) on yeast aerobic respiration was calculated through the comparison of aerobic respiration of simazine-exposed and non-exposed yeast cells...
November 15, 2018: Biosensors
Nadezhda A Byzova, Svetlana V Vinogradova, Elena V Porotikova, Uliana D Terekhova, Anatoly V Zherdev, Boris B Dzantiev
Grapevine leafroll-associated virus 3 (GLRaV-3) is one of the main pathogens of grapes, causing a significant loss in yield and decrease in quality for this agricultural plant. For efficient widespread control of this infection, rapid and simple analytical techniques of on-site testing are requested as a complementary addition for the currently applied hybridization (PCR) and immunoenzyme (ELISA) approaches. The given paper presents development and approbation of the immunochromatographic assay (ICA) for rapid detection of GLRaV-3...
November 15, 2018: Biosensors
Antonio Aires, Elena Lopez-Martinez, Aitziber L Cortajarena
Among all new nanomaterials, metal nanoclusters (NCs) have attracted special attention due to their interesting optical properties, among others. Metal NCs have been recently studied and used as sensors for different analytes. However, there is a need to explore the potential of these new sensors in a systematic manner and to develop new systems to broaden the possibilities that sensing offers to the industry. In this work, we show the potential use of repeat protein scaffolds as versatile templates for the synthesis and stabilization of various metal NCs, specifically Au, Ag, and CuNCs...
November 15, 2018: Biosensors
Judith Molinari, Laura Florez, Anahí Medrano, Leandro Monsalve, Gabriel Ybarra
In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow's milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes...
November 15, 2018: Biosensors
Cristina Tortolini, Paolo Bollella, Rosaceleste Zumpano, Gabriele Favero, Franco Mazzei, Riccarda Antiochia
A single-use electrochemical screen-printed electrode is reported based on biomimetic properties of nanoceria particles (CeNPs). The developed tool showed an easy approach compared to the classical spectrophotometric methods reported in literature in terms of ease of use, cost, portability, and unnecessary secondary reagents. The sensor allowed the detection of the total antioxidant capacity (TAC) in wine samples. The sensor has been optimized and characterized electrochemically and then tested with antioxidant compounds occurred in wine samples...
November 14, 2018: Biosensors
Ardalan Chaichi, Alisha Prasad, Manas Ranjan Gartia
Noninvasive and label-free vibrational spectroscopy and microscopy methods have shown great potential for clinical diagnosis applications. Raman spectroscopy is based on inelastic light scattering due to rotational and vibrational modes of molecular bonds. It has been shown that Raman spectra provide chemical signatures of changes in biological tissues in different diseases, and this technique can be employed in label-free monitoring and clinical diagnosis of several diseases, including cardiovascular studies...
November 12, 2018: Biosensors
Andrey N Kuzmin, Artem Pliss, Alex Rzhevskii, Adrian Lita, Mioara Larion
Raman microspectroscopy is a rapidly developing technique, which has an unparalleled potential for in situ proteomics, lipidomics, and metabolomics, due to its remarkable capability to analyze the molecular composition of live cells and single cellular organelles. However, the scope of Raman spectroscopy for bio-applications is limited by a lack of software tools for express-analysis of biomolecular composition based on Raman spectra. In this study, we have developed the first software toolbox for immediate analysis of intracellular Raman spectra using a powerful biomolecular component analysis (BCA) algorithm...
November 10, 2018: Biosensors
Heike Bartsch, Ralf Peipmann, Maren Klett, Dana Brauer, Andreas Schober, Jens Müller
Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrodes with high spatial resolution would deliver crucial information. A current limitation is the increasing impedance of microelectrodes with decreasing diameter. This study evaluates the suitability of thick film gold electrodes, pristine and coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT), for the detection of cell adhesion on the electrode surface...
November 2, 2018: Biosensors
Sandra Lara, André Perez-Potti
In biomedical science among several other growing fields, the detection of specific biological agents or biomolecular markers, from biological samples is crucial for early diagnosis and decision-making in terms of appropriate treatment, influencing survival rates. In this regard, immunosensors are based on specific antibody-antigen interactions, forming a stable immune complex. The antigen-specific detection antibodies (i.e., biomolecular recognition element) are generally immobilized on the nanomaterial surfaces and their interaction with the biomolecular markers or antigens produces a physico-chemical response that modulates the signal readout...
November 1, 2018: Biosensors
Anna Shirinskaya, Gilles Horowitz, Jonathan Rivnay, George G Malliaras, Yvan Bonnassieux
We develop a numerical model for the current-voltage characteristics of organic electrochemical transistors (OECTs) based on steady-state Poisson's, Nernst's and Nernst⁻Planck's equations. The model starts with the doping⁻dedoping process depicted as a moving front, when the process at the electrolyte⁻polymer interface and gradually moves across the film. When the polymer reaches its final state, the electrical potential and charge density profiles largely depend on the way the cations behave during the process...
October 31, 2018: Biosensors
Richard B M Schasfoort, Fikri Abali, Ivan Stojanovic, Gestur Vidarsson, Leon W M M Terstappen
SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature...
October 30, 2018: Biosensors
Yongbo Liang, Zhencheng Chen, Rabab Ward, Mohamed Elgendi
Blood pressure is a basic physiological parameter in the cardiovascular circulatory system. Long-term abnormal blood pressure will lead to various cardiovascular diseases, making the early detection and assessment of hypertension profoundly significant for the prevention and treatment of cardiovascular diseases. In this paper, we investigate whether or not deep learning can provide better results for hypertension risk stratification when compared to the classical signal processing and feature extraction methods...
October 26, 2018: Biosensors
Marta Maria Pereira da Silva Neves, Daniel Martín-Yerga
Individual (bio)chemical entities could show a very heterogeneous behaviour under the same conditions that could be relevant in many biological processes of significance in the life sciences. Conventional detection approaches are only able to detect the average response of an ensemble of entities and assume that all entities are identical. From this perspective, important information about the heterogeneities or rare (stochastic) events happening in individual entities would remain unseen. Some nanoscale tools present interesting physicochemical properties that enable the possibility to detect systems at the single-entity level, acquiring richer information than conventional methods...
October 26, 2018: Biosensors
Tory M Schaaf, Ang Li, Benjamin D Grant, Kurt Peterson, Samantha Yuen, Prachi Bawaskar, Evan Kleinboehl, Ji Li, David D Thomas, Gregory D Gillispie
We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green and red FRET pairs (GFP/RFP), FPs that emit at longer wavelengths (orange and maroon, OFP/MFP) increased the FRET efficiency, dynamic range, and signal-to-background of high-throughput screening (HTS). OFP and MFP were fused to specific sites on the human cardiac calcium pump (SERCA2a) for detection of structural changes due to small-molecule effectors...
October 24, 2018: Biosensors
Ngoc-Viet Nguyen, Chun-Hao Yang, Chung-Jung Liu, Chao-Hung Kuo, Deng-Chyang Wu, Chun-Ping Jen
Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths. In this work, an electrical sensing platform is built based on amine-terminated aptamer modified-gold electrodes for the specific, label-free detection of a human lung carcinoma cell line (A549). The microdevice, that includes a coplanar electrodes configuration and a simple microfluidic channel on a glass substrate, is fabricated using standard photolithography and cast molding techniques...
October 20, 2018: Biosensors
Peijun J W He, Ioannis N Katis, Robert W Eason, Collin L Sones
Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution-detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique...
October 20, 2018: Biosensors
Joost Nelis, Christopher Elliott, Katrina Campbell
A human mission to Mars can be viewed as the apex of human technological achievement. However, to make this dream a reality several obstacles need to be overcome. One is devising practical ways to safeguard the crew health during the mission through the development of easy operable and compact sensors. Lately, several smartphone-based sensing devices (SBDs) with the purpose to enable the immediate sensitive detection of chemicals, proteins or pathogens in remote settings have emerged. In this critical review, the potential to piggyback these systems for in situ analysis in space has been investigated on application of a systematic keyword search whereby the most relevant articles were examined comprehensively and existing SBDs were divided into 4 relevant groups for the monitoring of crew health during space missions...
October 19, 2018: Biosensors
Muhammad Imran, Muhammad Ramzan, Ahmad Kaleem Qureshi, Muhammad Azhar Khan, Muhammad Tariq
In recent years, scientific advancements have constantly increased at a significant rate in the field of biomedical science. Keeping this in view, the application of porphyrins and metalloporphyrins in the field of biomedical science is gaining substantial importance. Porphyrins are the most widely studied tetrapyrrole-based compounds because of their important roles in vital biological processes. The cavity of porphyrins containing four pyrrolic nitrogens is well suited for the binding majority of metal ions to form metalloporphyrins...
October 19, 2018: Biosensors
Karolina Dziąbowska, Elżbieta Czaczyk, Dawid Nidzworski
The basic affairs connected to the influenza virus were reviewed in the article, highlighting the newest trends in its diagnostic methods. Awareness of the threat of influenza arises from its ability to spread and cause a pandemic. The undiagnosed and untreated viral infection can have a fatal effect on humans. Thus, the early detection seems pivotal for an accurate treatment, when vaccines and other contemporary prevention methods are not faultless. Public health is being attacked with influenza containing new genes from a genetic assortment between animals and humankind...
October 18, 2018: Biosensors
Odd Martin Staal, Heidi Marie Umbach Hansen, Sverre Christian Christiansen, Anders Lyngvi Fougner, Sven Magnus Carlsen, Øyvind Stavdahl
Freestyle Libre (FL) is a factory calibrated Flash Glucose Monitor (FGM). We investigated Mean Absolute Relative Difference (MARD) between Self Monitoring of Blood Glucose (SMBG) and FL measurements in the first day of sensor wear in 39 subjects with Type 1 diabetes. The overall MARD was 12.3%, while the individual MARDs ranged from 4% to 25%. Five participants had a MARD ≥ 20%. We estimated bias and lag between the FL and SMBG measurements. The estimated biases range from -1.8 mmol / L to 1.4 mmol / L , and lags range from 2 min to 24 min ...
October 17, 2018: Biosensors
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