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Methods and Applications in Fluorescence

Alizé Proust, Julie-Christine Lévesque, Corinne Barat, Sachiko Sato, Michel J Tremblay
Extracellular traps ejected by various immune cells (neutrophils, macrophages, eosinophils and mast cells) have several immune functions, either protective against pathogens or deleterious in some autoimmune or inflammatory disorders. Since their first description in 2004, the mechanisms of extracellular traps formation have been extensively investigated though still not fully understood. We describe here a new tool for the detection of extracellular traps by fluorescence microscopy in a single-step staining protocol, which does not require any wash...
May 16, 2018: Methods and Applications in Fluorescence
Rukiye Ayranci, Fatma Ozturk Kirbay, Dilek Odaci Demirkol, Metin Ak, Suna Timur
Simple, rapid and effective fluorescence sensing platform have been fabricated using fluorescent conducting polymer surface. For this purpose, a rhodamine based electroactive monomer (RDC) and a functional group containing monomer (SNS) have been copolymerized to develop a conducting polymer based sensor platform having a fluorescence and enzyme-binding surface on ITO electrode. The proposed fluorescence sensing mechanism for detection glucose is related to the consumption of dissolved oxygen at double layer of the electrode which is fluorescence quenching agent by glucose-GOx reaction...
May 16, 2018: Methods and Applications in Fluorescence
Kyusik Yun, Linlin Zhong
A novel fluorescence "Switch on" for the detection of heparin based on the RhB-COL/GO system was achieved. A strong fluorescence dye, Rhodamine B, was modified by chitosan oligosaccharide lactate (COL), which plays a major role in the formation of a positively charged RhB-COL complex. RhB-COL was soluble and stable in solution, which was characterized by using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. GO sheets quenched the fluorescence intensity of RhB-COL due to electron transfer from RhB to the GO surface...
May 16, 2018: Methods and Applications in Fluorescence
James Manton, Yao Xiao, Robert Turner, Graham Christie, Eric Rees
It is often necessary to precisely quantify the size of specimens in biological studies. When measuring feature size in fluorescence microscopy, significant biases can arise due to blurring of its edges if the feature is smaller than the diffraction limit of resolution. This problem is avoided if an equation describing the feature's entire image is fitted to its image data. In this paper we present open-source software, ELM, which uses this approach to measure the size of spheroidal or cylindrical fluorescent shells with a precision of around 10 nm...
May 4, 2018: Methods and Applications in Fluorescence
Xiaohui Wang, Hongshang Peng, Kun Cheng, Xiaoming Liu, Yuanan Liu, Wei Yang
Ratiometric fluorescent nanoparticles (NPs) under two-photon excitation are successfully developed for sensing dissolved oxygen. The NPs comprise the oxygen probe Pt(II)-porphyrins (PtTFPP) and fluorescent organic semiconducting polymer (PFO). PFO polymer acts as both a two-photon antenna and a reference dye, while PtTFPP absorbs the photonic energy transferred by the PFO under two-photon excitation at 740 nm to sense oxygen. The red fluorescence of PtTFPP is sensitive to oxygen with a quenching response of 88% from nitrogen saturation to oxygen saturation, and PFO gives oxygen-insensitive referenced blue fluorescence...
April 13, 2018: Methods and Applications in Fluorescence
Kirti Kumari Sharma, Gavash Harsha Kannikanti, Tulsida R Baggi, Jayathirtha Rao Vaidya
Present work is conducted to demonstrate the use of pyrene for the development of latent fingermarks. Pyrene formulation with binders can be efficiently used for developing latent fingermarks on porous, non-porous and semi-porous surfaces. The effectiveness of pyrene formulation for the detection of latent fingermarks present on a large variety of objects was systematically and comparatively carried out. To optimize the working formulation, studies were carried out using different pyrene concentrations followed by various substrate study, time dependent study, temperature study, depleted fingermark development and the stability of the proposed formulation...
April 13, 2018: Methods and Applications in Fluorescence
Anita R Warrier, R Gandhimathi
In this article, we report on enhancement in photoluminescence and Raman line intensity of SnS quantum dots embedded in a mesh of Sn nanostructures. SnS nanoparticles synthesized by homogenous precipitation method show strong quantum confinement with a band gap of ~ 2.7 eV (blue shift of ~ 1 eV compared to bulk SnS particles). The optical band gap of SnS quantum dots is controlled by varying the pH (~ 0 to 2.25), ageing time 
 (24 to 144 hours) and molarity (0 to 2 M) of the precursors. These SnS nanoparticles are embedded in a mesh of Sn nanostructures which are synthesized from tin chloride by using sodium borohydride as reducing agent...
April 10, 2018: Methods and Applications in Fluorescence
Ming Jun Cen, Tao Chang, Yao Bing Yin, Xiao Liang Li, Yong Wu He
A novel ratiometric fluorescent probe <b>BCB</b> was reported for the first time for the sensitive and selective analysis of Ag<sup>+</sup> based on two Boradiazaindacene (BODIPY) as both the reference fluorophore and recognition part. Upon the addition of Ag<sup>+</sup> ions, a strong fluorescence emission at 570 nm from recognition BODIPY would be appeared, whereas the fluorescence of the other BODIPY at 535 nm would be a constant reference fluorescence signal...
April 10, 2018: Methods and Applications in Fluorescence
Aurimas Vysniauskas, Ismael Lopez Duarte, Alex J Thompson, James A Bull, Marina Kuimova
Surface functionalisation with viscosity sensitive dyes termed 'molecular rotors' can potentially open up new opportunities in sensing, for example for non-invasive biological viscosity imaging, in studying the effect of shear stress on lipid membranes and in cells, and in imaging contacts between surfaces upon applied pressure. We have functionalised microscope slides with BODIPY-based molecular rotor capable of viscosity sensing via its fluorescence lifetime. We have optimised functionalisation conditions and prepared the slides with the BODIPY rotor attached directly to the surface of glass slides and through linkers of ∼40 nm and ∼300 nm in length...
April 3, 2018: Methods and Applications in Fluorescence
Ratneshwar Kumar Ratnesh, Mohan Singh Mehata
We have prepared stable colloidal CdTe and CdTe/ZnS core-shell quantum dots (QDs) using hot injection chemical route. The developed CdTe QDs emit tunable single and dual photoluminescence (PL) bands, originating from the direct band edge and the surface state of QDs, as evident by the steady-state and time-resolved spectroscopy. The developed CdTe and CdTe/ZnS QDs act as optical sensors for the detection of metal ions (e.g., Fe2+ and Pb2+) in the feed water. The PL quenching in the presence of analytes has been examined by both the steady-state and time-resolved PL spectroscopy...
March 29, 2018: Methods and Applications in Fluorescence
Christine Guzman, Xue Han, Eiichi Shoguchi, Sile Nic Chormaic
The partnership between coral and its algal symbionts, Symbiodinium, is crucial to the global environment. Yet, the regulatory process within the photosynthetic machinery of Symbiodinium is still not clearly understood. Here, we studied the influence of light stress from focused red and blue lasers on single Symbiodinium cells. Fluorescence signals were measured to show cell response. Increasing the incident laser power or the exposure time resulted in an increase followed by a decline in fluorescence intensity...
March 29, 2018: Methods and Applications in Fluorescence
Vladimir Tomin
Alternative interpretations of some results obtained in the paper of A. Szalai et al.: Temperature dependent spectroscopic and excited state dynamics of 3-hydroxychromones with electron donor and acceptor substituents, in Methods and applications in Fluorescence. 5(2017) 024011, were given.
 The main points of the criticism are listed below. 
 Physical characteristics of the experimental set up using excitation pulses shorter than 0.15 ns impose certain constrains on accuracy of measurements...
March 23, 2018: Methods and Applications in Fluorescence
Ying Lian, Long-Jiang Ding, Wei Zhang, Xiao-Ai Zhang, Ying-Lu Zhang, Zhen-Zhen Lin, Xu-Dong Wang
Cyanine dyes are widely used in biological labeling and imaging because of their narrow near infrared emission, good brightness, and high flexibility in functionalization, which not only enables multiplex analysis and multi-color imaging, but also greatly reduces autofluorescence from biological matter and increases signal-to-noise ratio. Unfortunately, their poor chemical- and photo-stability strongly limits their applications. The incorporation of cyanine dyes in silica nanoparticles provides a solution to the problem...
March 23, 2018: Methods and Applications in Fluorescence
Dimitri Guala, Kristoffer Bernhem, Hammou Ait Blal, Daniel Jans, Emma Lundberg, Hjalmar Brismar, Erik L L Sonnhammer
Huge amounts of data are generated in genome wide experiments, designed to investigate diseases with complex genetic causes. Follow up of all potential leads produced by such experiments is currently cost prohibitive and time consuming. Gene prioritization tools alleviate these constraints by directing further experimental efforts towards the most promising candidate targets. Recently a gene prioritization tool called MaxLink was shown to outperform other widely used state-of-the-art prioritization tools in a large scale in silico benchmark...
March 23, 2018: Methods and Applications in Fluorescence
Nicholas Nunn, Marta d'Amora, Neeraj Prabhakar, Alexander M Panich, Natalya Froumin, Marco D Torelli, Igor Vlasov, Philipp Reineck, Brant Gibson, Jessica M Rosenholm, Silvia Giordani, Olga Shenderova
Detonation nanodiamonds (DNDs) have emerged as promising candidates for a variety of biomedical applications, thanks to different physicochemical and biological properties, such as small size and reactive surfaces. In this study, we propose carbon dot decorated single digit (4-5 nm diameter) primary particles of detonation nanodiamond as promising fluorescent probes. Due to their intrinsic fluorescence originating from tiny (1-2 atomic layer thickness) carbonaceous structures on their surfaces, they exhibit brightness suitable for in vitro imaging...
May 9, 2018: Methods and Applications in Fluorescence
A Dwivedi, K Mishra, S B Rai
This work presents the spectral conversion characteristics [upconversion (UC), downshifting (DS) and quantum-cutting (QC) optical processes] of Eu3+ , Yb3+ and Bi3+ co-doped LaNbO4 (LBO) phosphor samples synthesized by solid state reaction technique. The crystal structure and the pure phase formation have been confirmed by x-ray diffraction (XRD) measurements. The surface morphology and particle size are studied by scanning electron microscopy (SEM). The rarely observed intense red UC emission from Eu3+ ion has been successfully obtained in Eu3+ /Yb3+ co-doped LaNbO4 phosphor (on excitation with 980 nm) by optimizing the concentrations of Eu3+ and Yb3+ ions...
April 4, 2018: Methods and Applications in Fluorescence
Shambhavi Katyayan, Sadhana Agrawal
This paper reports structural investigations of rare earth doped BaZrO3 phosphors synthesized by Solid state reaction technique with varying concentrations of Eu3+ and Tb3+ from 0 mol% to 2 mol%. The synthesized phosphors show enhanced variable emissions in the visible region corresponding to different hypersensitive electronic transitions of Eu3+ and Tb3+ ions. With cubic structure confirmed in XRD analysis, the FESEM images show uniform grain connectivity and homogeneity of prepared samples. The TEM micrographs of the synthesized phosphors show agglomerated irregular structures...
April 4, 2018: Methods and Applications in Fluorescence
J Vangindertael, R Camacho, W Sempels, H Mizuno, P Dedecker, K P F Janssen
Ever since the inception of light microscopy, the laws of physics have seemingly thwarted every attempt to visualize the processes of life at its most fundamental, sub-cellular, level. The diffraction limit has restricted our view to length scales well above 250 nm and in doing so, severely compromised our ability to gain true insights into many biological systems. Fortunately, continuous advancements in optics, electronics and mathematics have since provided the means to once again make physics work to our advantage...
March 16, 2018: Methods and Applications in Fluorescence
Jiajia Shan, Xue Wang, Hao Zhou, Shuqing Han, Dimas Firmanda Al Riza, Naoshi Kondo
Synchronous fluorescence spectra, combined with multivariate analysis were used to predict flavonoids content in green tea rapidly and nondestructively. This paper presented a new and efficient spectral intervals selection method called clustering based partial least square (CL-PLS), which selected informative wavelengths by combining clustering concept and partial least square (PLS) methods to improve models' performance by synchronous fluorescence spectra. The fluorescence spectra of tea samples were obtained and k-means and kohonen-self organizing map clustering algorithms were carried out to cluster full spectra into several clusters, and sub-PLS regression model was developed on each cluster...
March 13, 2018: Methods and Applications in Fluorescence
Enrico Rampazzo, Damiano Genovese, Francesco Palomba, Luca Prodi, Nelsi Zaccheroni
The development of nanostructures devoted to in vivo imaging and theranostic applications is one of the frontier fields of research worldwide. In this context, silica nanoparticles (SiO2 -NPs) offer unquestionable positive properties: silica is intrinsically non-toxic, several versatile and accessible synthetic methods are available and many variations are possible, both in terms of porosity and functionalization for delivery and targeting purposes, respectively. Moreover, the accumulation of several dyes within a single nanostructure offers remarkable possibilities to produce very bright and photostable luminescent nanosystems...
March 13, 2018: Methods and Applications in Fluorescence
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