journal
MENU ▼
Read by QxMD icon Read
search

Mass Spectrometry

journal
https://www.readbyqxmd.com/read/27900236/identification-of-two-sulfated-cholesterol-metabolites-found-in-the-urine-of-a-patient-with-niemann-pick-disease-type-c-as-novel-candidate-diagnostic-markers
#1
Masamitsu Maekawa, Kaoru Omura, Shoutaro Sekiguchi, Takashi Iida, Daisuke Saigusa, Hiroaki Yamaguchi, Nariyasu Mano
In the urine of a Niemann-Pick disease type C (NPC) patient, we have identified three characteristic intense peaks that have not been observed in the urine of a 3β-hydroxysteroid-Δ(5)-C27-steroid dehydrogenase deficiency patient or a healthy infant and adult. Based on accurate masses of the protonated molecules, we focused on two of them as candidate NPC diagnostic markers. Two synthesized authentic preparations agreed with the two compounds found in NPC patient urine in regard to both chromatographic behavior and accurate masses of the deprotonated molecules...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27900235/technical-challenges-in-mass-spectrometry-based-metabolomics
#2
Fumio Matsuda
Metabolomics is a strategy for analysis, and quantification of the complete collection of metabolites present in biological samples. Metabolomics is an emerging area of scientific research because there are many application areas including clinical, agricultural, and medical researches for the biomarker discovery and the metabolic system analysis by employing widely targeted analysis of a few hundred preselected metabolites from 10-100 biological samples. Further improvement in technologies of mass spectrometry in terms of experimental design for larger scale analysis, computational methods for tandem mass spectrometry-based elucidation of metabolites, and specific instrumentation for advanced bioanalysis will enable more comprehensive metabolome analysis for exploring the hidden secrets of metabolism...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27900234/n-terminal-derivatization-with-structures-having-high-proton-affinity-for-discrimination-between-leu-and-ile-residues-in-peptides-by-high-energy-collision-induced-dissociation
#3
Atsushi Kitanaka, Masahiro Miyashita, Ayumi Kubo, Takaya Satoh, Michisato Toyoda, Hisashi Miyagawa
De novo sequencing is still essential in the identification of peptides and proteins from unexplored organisms whose sequence information is not available. One of the remaining problems in de novo sequencing is discrimination between Leu and Ile residues. The discrimination is possible based on differences in side chain fragmentation between Leu and Ile under high-energy collision-induced dissociation (HE-CID) conditions. However, this is observed only when basic residues, such as Arg and Lys, are present near the N- or C-terminal end...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27843740/verification-of-ribosomal-proteins-of-aspergillus-fumigatus-for-use-as-biomarkers-in-maldi-tof-ms-identification
#4
Sayaka Nakamura, Hiroaki Sato, Reiko Tanaka, Takashi Yaguchi
We have previously proposed a rapid identification method for bacterial strains based on the profiles of their ribosomal subunit proteins (RSPs), observed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method can perform phylogenetic characterization based on the mass of housekeeping RSP biomarkers, ideally calculated from amino acid sequence information registered in public protein databases. With the aim of extending its field of application to medical mycology, this study investigates the actual state of information of RSPs of eukaryotic fungi registered in public protein databases through the characterization of ribosomal protein fractions extracted from genome-sequenced Aspergillus fumigatus strains Af293 and A1163 as a model...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27800293/multi-stage-mass-spectrometry-of-poly-vinyl-pyrrolidone-and-its-vinyl-succinimide-copolymer-formed-upon-exposure-to-sodium-hypochlorite
#5
Thierry Fouquet, Masaki Torimura, Hiroaki Sato
The degradation routes of poly(vinyl pyrrolidone) (PVP) exposed to sodium hypochlorite (bleach) have been previously investigated using chemical analyses such as infrared spectroscopy. So far, no reports have proposed mass spectrometry (MS) as an alternative tool despite its capability to provide molecular and structural information using its single stage electrospray (ESI) or matrix assisted laser desorption ionization (MALDI) and multi stage (MS (n) ) configurations, respectively. The present study thus reports on the characterization of PVP after its exposure to bleach by high resolution MALDI spiralTOF-MS and Kendrick mass defect analysis providing clues as to the formation of a vinyl pyrrolidone/vinyl succinimide copolymeric degradation product...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27656337/a-method-for-simultaneous-determination-of-25-hydroxyvitamin-d3-and-its-3-sulfate-in-newborn-plasma-by-lc-esi-ms-ms-after-derivatization-with-a-proton-affinitive-cookson-type-reagent
#6
Tatsuya Higashi, Mai Yokota, Ayaka Goto, Kenji Komatsu, Takahiro Sugiura, Shoujiro Ogawa, Mamoru Satoh, Fumio Nomura
A method for the simultaneous determination of 25-hydroxyvitamin D3 [25(OH)D3] and its 3-sulfate [25(OH)D3S] in newborn plasma, which is expected to be helpful in the assessment of the vitamin D status, using stable isotope-dilution liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) has been developed and validated. The plasma was pretreated based on the deproteinization and solid-phase extraction, then subjected to derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD)...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27563510/photochemical-reactions-of-aminonaphthols-caused-by-laser-desorption-ionization
#7
Keishiro Nagoshi, Kazuma Inatomi, Issey Osaka, Mitsuo Takayama
The formation of monomeric and dimeric ions of seven different aminonaphthols (ANLs) has been studied by using laser desorption/ionization (LDI) with a nitrogen laser. The positive-ion data of all the ANLs merely showed molecular ion M(·+) without protonated molecule [M+H](+), while 1-amino-2-naphthol (1,2-ANL) and 2-amino-1-naphthol (2,1-ANL) showed an intense dimeric ion [2 M-2H2O+H](+). The negative-ion data showed deprotonated molecule [M-H](-) in common, while the spectra of 1,2-ANL, 2,1-ANL and 8-amino-2-naphthol (8,2-ANL) accompanied an intense peak corresponding to negative molecular ion M(·-) and the 8,2-ANL and 4-amino-1-naphthol (4,1-ANL) accompanied dehydrogenated anion [M-2H](·-)...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27446770/metabolomic-analysis-of-gingival-crevicular-fluid-using-gas-chromatography-mass-spectrometry
#8
Miho Ozeki, Takenori Nozaki, Jun Aoki, Takeshi Bamba, Kirk R Jensen, Shinya Murakami, Michisato Toyoda
Periodontitis is one of the most prevalent threats to oral health as the most common cause of tooth loss. In order to perform effective treatment, a clinical test that detect sites where disease activity is high and predicts periodontal tissue destruction is strongly desired, however, it is still difficult to prognose the periodontal tissue breakdown on the basis of conventional methods. The aim of this study is to examine the usefulness of gas chromatography/mass spectrometry (GC/MS), which could eventually be used for on-site analysis of metabolites in gingival crevicular fluid (GCF) in order to objectively diagnose periodontitis at a molecular level...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27313979/rapid-and-accurate-identification-of-animal-species-in-natural-leather-goods-by-liquid-chromatography-mass-spectrometry
#9
Yukari Izuchi, Tsuneo Takashima, Naoya Hatano
The demand for leather goods has grown globally in recent years. Industry revenue is forecast to reach $91.2 billion by 2018. There is an ongoing labelling problem in the leather items market, in that it is currently impossible to identify the species that a given piece of leather is derived from. To address this issue, we developed a rapid and simple method for the specific identification of leather derived from cattle, horses, pigs, sheep, goats, and deer by analysing peptides produced by the trypsin-digestion of proteins contained in leather goods using liquid chromatography/mass spectrometry...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27313978/identification-of-anthropogenic-compounds-in-urban-environments-and-evaluation-of-automated-methods-for-reading-fragmentation-a-case-of-river-water
#10
Atsushi Yamamoto, Naoko Matsumoto, Hideya Kawasaki, Ryuichi Arakawa
A workflow based on liquid chromatography/high-resolution mass spectrometry (LC/HR-MS) was applied for the identification of compounds in urban environments. Substances extracted by solid-phase extraction from river water were wholly analyzed by LC/HR-MS without any purification. Fragmentation in collision-induced dissociation was manually studied for the 20 most intense ions in positive- and negative-ion electrospray ionization with accurate mass determination at a resolution of 100,000. Sixteen anthropogenic compounds in the extract were identified and confirmed using standard reference reagents...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27162707/maldi-in-source-decay-of-protein-the-mechanism-of-c-ion-formation
#11
REVIEW
Mitsuo Takayama
The in-source decay (ISD) phenomenon, the fragmentation at an N-Cα bond of a peptide backbone that occurs within several tens of nanoseconds in the ion-source in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), is discussed from the standpoints of the discovery and early publications dealing with MALDI-ISD, the formation of c-ions in energy-sudden desorption/ionization methods, the formation of radical species in a MALDI, model construction for ISD, and matrix materials that are suitable for use in MALDI-ISD...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27162706/determination-of-detection-limits-and-quantitation-limits-for-compounds-in-a-database-of-gc-ms-by-fumi-theory
#12
Shinya Nakashima, Yuzuru Hayashi
The aim of this paper is to propose a stochastic method for estimating the detection limits (DLs) and quantitation limits (QLs) of compounds registered in a database of a GC/MS system and prove its validity with experiments. The approach described in ISO 11843 Part 7 is adopted here as an estimation means of DL and QL, and the decafluorotriphenylphosphine (DFTPP) tuning and retention time locking are carried out for adjusting the system. Coupled with the data obtained from the system adjustment experiments, the information (noise and signal of chromatograms and calibration curves) stored in the database is used for the stochastic estimation, dispensing with the repetition measurements...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/26819913/conifer-derived-monoterpenes-and-forest-walking
#13
Kazuhiro Sumitomo, Hiroaki Akutsu, Syusei Fukuyama, Akiho Minoshima, Shin Kukita, Yuji Yamamura, Yoshiaki Sato, Taiki Hayasaka, Shinobu Osanai, Hiroshi Funakoshi, Naoyuki Hasebe, Masao Nakamura
Conifer and broadleaf trees emit volatile organic compounds in the summer. The major components of these emissions are volatile monoterpenes. Using solid phase microextraction fiber as the adsorbant, monoterpenes were successfully detected and identified in forest air samples. Gas chromatography/mass chromatogram of monoterpenes in the atmosphere of a conifer forest and that of serum from subjects who were walking in a forest were found to be similar each other. The amounts of α-pinene in the subjects became several folds higher after forest walking...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819912/when-api-mass-spectrometry-meets-super-atmospheric-pressure-ion-sources
#14
Lee Chuin Chen
In a tutorial paper on the application of free-jet technique for API-MS, John Fenn mentioned that "…for a number of years and a number of reasons, it has been found advantageous in many situations to carry out the ionization process in gas at pressures up to 1000 Torr or more" (Int. J. Mass Spectrom. 200: 459-478, 2000). In fact, the first ESI mass spectrometer constructed by Yamashita and Fenn had a counter-flow curtain gas source at 1050 Torr (ca. 1.4 atm) to sweep away the neutral (J. Phys. Chem. 88: 4451-4459, 1984)...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819911/development-of-an-imaging-mass-spectrometry-technique-for-visualizing-localized-cellular-signaling-mediators-in-tissues
#15
Yuki Sugiura, Kurara Honda, Makoto Suematsu
In vivo concentrations of cellular signaling mediators such as inflammatory mediators are normally maintained at very low levels due to their strong ability to induce a biological response. The production, diffusion, and decomposition of such mediators are spatio-temporally regulated. Therefore, in order to understand biochemical basis of disease progression and develop new therapeutic strategies, it is important to understand the spatiotemporal dynamics of the signaling mediators in vivo, during the progression of disorders, e...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819910/middle-down-and-chemical-proteomic-approaches-to-reveal-histone-h4-modification-dynamics-in-cell-cycle-label-free-semi-quantification-of-histone-tail-peptide-modifications-including-phosphorylation-and-highly-sensitive-capture-of-histone-ptm-binding-proteins
#16
Kazuki Yamamoto, Yoko Chikaoka, Gosuke Hayashi, Ryosuke Sakamoto, Ryuji Yamamoto, Akira Sugiyama, Tatsuhiko Kodama, Akimitsu Okamoto, Takeshi Kawamura
Mass spectrometric proteomics is an effective approach for identifying and quantifying histone post-translational modifications (PTMs) and their binding proteins, especially in the cases of methylation and acetylation. However, another vital PTM, phosphorylation, tends to be poorly quantified because it is easily lost and inefficiently ionized. In addition, PTM binding proteins for phosphorylation are sometimes resistant to identification because of their variable binding affinities. Here, we present our efforts to improve the sensitivity of detection of histone H4 tail peptide phosphorylated at serine 1 (H4S1ph) and our successful identification of an H4S1ph binder candidate by means of a chemical proteomics approach...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819909/probe-heating-method-for-the-analysis-of-solid-samples-using-a-portable-mass-spectrometer
#17
Shun Kumano, Masuyuki Sugiyama, Masuyoshi Yamada, Kazushige Nishimura, Hideki Hasegawa, Hidetoshi Morokuma, Hiroyuki Inoue, Yuichiro Hashimoto
We previously reported on the development of a portable mass spectrometer for the onsite screening of illicit drugs, but our previous sampling system could only be used for liquid samples. In this study, we report on an attempt to develop a probe heating method that also permits solid samples to be analyzed using a portable mass spectrometer. An aluminum rod is used as the sampling probe. The powdered sample is affixed to the sampling probe or a droplet of sample solution is placed on the tip of the probe and dried...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819908/maldi-matrix-research-for-biopolymers
#18
Yuko Fukuyama
Matrices are necessary materials for ionizing analytes in matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). The choice of a matrix appropriate for each analyte controls the analyses. Thus, in some cases, development or improvement of matrices can become a tool for solving problems. This paper reviews MALDI matrix research that the author has conducted in the recent decade. It describes glycopeptide, carbohydrate, or phosphopeptide analyses using 2,5-dihydroxybenzoic acid (2,5-DHB), 1,1,3,3-tetramethylguanidinium (TMG) salts of p-coumaric acid (CA) (G3CA), 3-aminoquinoline (3-AQ)/α-cyano-4-hydroxycinnamic acid (CHCA) (3-AQ/CHCA) or 3-AQ/CA and gengeral peptide, peptide containing disulfide bonds or hydrophobic peptide analyses using butylamine salt of CHCA (CHCAB), 1,5-diaminonaphthalene (1,5-DAN), octyl 2,5-dihydroxybenzoate (alkylated dihydroxybenzoate, ADHB), or 1-(2,4,6-trihydroxyphenyl)octan-1-one (alkylated trihydroxyacetophenone, ATHAP)...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819907/method-for-the-compound-annotation-of-conjugates-in-nontargeted-metabolomics-using-accurate-mass-spectrometry-multistage-product-ion-spectra-and-compound-database-searching
#19
Tairo Ogura, Takeshi Bamba, Akihiro Tai, Eiichiro Fukusaki
Owing to biotransformation, xenobiotics are often found in conjugated form in biological samples such as urine and plasma. Liquid chromatography coupled with accurate mass spectrometry with multistage collision-induced dissociation provides spectral information concerning these metabolites in complex materials. Unfortunately, compound databases typically do not contain a sufficient number of records for such conjugates. We report here on the development of a novel protocol, referred to as ChemProphet, to annotate compounds, including conjugates, using compound databases such as PubChem and ChemSpider...
2015: Mass Spectrometry
https://www.readbyqxmd.com/read/26819906/characterization-of-mycolic-acids-in-total-fatty-acid-methyl-ester-fractions-from-mycobacterium-species-by-high-resolution-maldi-tofms
#20
Kanae Teramoto, Mitsuo Suga, Takafumi Sato, Takayuki Wada, Atsushi Yamamoto, Nagatoshi Fujiwara
Mycolic acids (MAs) are characteristic components of bacteria in the suborder Corynebacterineae, such as Mycobacterium. MAs are categorized into subclasses based on their functional bases (cyclopropane ring, methoxy, keto, and epoxy group). Since MAs have heterogeneity among bacterial species, analyzing of MAs are required in the chemotaxonomic field. However, their structural analysis is not easy because of their long carbon-chain lengths and several functional groups. In this study, total fatty acid (FA) methyl ester (ME) fraction of M...
2015: Mass Spectrometry
journal
journal
47862
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"