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Advanced Pharmaceutical Bulletin

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https://www.readbyqxmd.com/read/28101473/purification-and-characterization-of-bovine-serum-albumin-using-chromatographic-method
#1
Sanaz Balkani, Sara Shamekhi, Ramin Raoufinia, Reza Parvan, Jalal Abdolalizadeh
Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification,the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101472/the-effects-of-hypoxia-on-u937-cell-line-in-mesenchymal-stem-cells-co-culture-system
#2
Mostafa Ejtehadifar, Karim Shamsasenjan, Parvin Akbarzadehlaleh, Sarah Zahedi, Narjes Kazemi
Purpose: Mesenchymal Stem Cells (MSCs) are the most important members of Bone Marrow (BM) milieu. MSCs affect different kinds of cells, particularly malignant cells of hematologic malignancies, but the effects of MSCs are unclear exactly. Here we analyzed the effects of derived Umbilical Cord Blood-MSCs on proliferation, cell death and some surface markers of U937 cell line in a Co-culture system with MSCs. Methods: Here we designed Co-culture systems as a model of BM milieu. We cultured U937 cells on UCB-MSCs and MSCs Conditioned Medium (C...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101471/association-of-vitamin-d-deficiency-and-thyroid-function-in-postmenopausal-women
#3
Mitra Niafar, Leili Pourafkari, Saina Aminmozaffari, Nader D Nader
Pupose: Although there are reports of vitamin D (VitD) insufficiency in immune-mediated hypothyroidism, an association between VitD and thyroid-stimulating hormone (TSH) levels has yet to be shown. We aim to examine VitD and TSH levels among postmenopausal women, as both conditions are more prevalent in elderly women. Methods: The clinic records of postmenopausal women during their routine maintenance visits were reviewed. All patients were examined for the symptoms related to thyroid function and osteoporosis...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101470/protective-roles-of-n-acetyl-cysteine-and-or-taurine-against-sumatriptan-induced-hepatotoxicity
#4
Javad Khalili Fard, Hossein Hamzeiy, Mohammadreza Sattari, Mohammad Ali Eghbal
Purpose: Triptans are the drug category mostly prescribed for abortive treatment of migraine. Most recent cases of liver toxicity induced by triptans have been described, but the mechanisms of liver toxicity of these medications have not been clear. Methods: In the present study, we obtained LC50 using dose-response curve and investigated cell viability, free radical generation, lipid peroxide production, mitochondrial injury, lysosomal membrane damage and the cellular glutathione level as toxicity markers as well as the beneficial effects of taurine and/or N-acetyl cysteine in the sumatriptan-treated rat parenchymal hepatocytes using accelerated method of cytotoxicity mechanism screening...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101469/concurrent-inflammation-augments-antimalarial-drugs-induced-liver-injury-in-rats
#5
Hossein Niknahad, Reza Heidari, Roya Firuzi, Farzaneh Abazari, Maral Ramezani, Negar Azarpira, Massood Hosseinzadeh, Asma Najibi, Arastoo Saeedi
Purpose: Accumulating evidence suggests that drug exposure during a modest inflammation induced by bacterial lipopolysaccharide (LPS) might increase the risk of drug-induced liver injury. The current investigation was designed to test if antimalarial drugs hepatotoxicity is augmented in LPS‑treated animals. Methods: Rats were pre-treated with LPS (100 µg/kg, i.p). Afterward, non-hepatotoxic doses of amodiaquine (25, 50 and 100 mg/kg, oral) and chloroquine (25, 50 and 100 mg/kg, oral) were administered. Results: Interestingly, liver injury was evident only in animals treated with both drug and LPS as estimated by pathological changes in serum biochemistry (ALT, AST, LDH, and TNF-α), and liver tissue (severe hepatitis, endotheliitis, and sinusoidal congestion)...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101468/agglomeration-of-celecoxib-by-quasi-emulsion-solvent-diffusion-method-effect-of-stabilizer
#6
Maryam Maghsoodi, Ali Nokhodchi
Purpose: The quasi-emulsion solvent diffusion (QESD) has evolved into an effective technique to manufacture agglomerates of API crystals. Although, the proposed technique showed benefits, such as cost effectiveness, that is considerably sensitive to the choice of a stabilizer, which agonizes from a absence of systemic understanding in this field. In the present study, the combination of different solvents and stabilizers were compared to investigate any connections between the solvents and stabilizers. Methods: Agglomerates of celecoxib were prepared by QESD method using four different stabilizers (Tween 80, HPMC, PVP and SLS) and three different solvents (methyl acetate, ethyl acetate and isopropyl acetate)...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101467/some-qualitative-and-rheological-properties-of-virgin-olive-oil-apple-vinegar-salad-dressing-stabilized-with-xanthan-gum
#7
Solmaz Abedinzadeh, Mohammadali Torbati, Sodeif Azadmard-Damirchi
Purpose: Lipid oxidation and rheological properties are the main qualitative parameters determined in food emulsions. Salad dressings are food emulsions important in our daily diet, but conventional salad dressings have high amounts of cholesterol and saturated fatty acids because of egg yolk in their formulations. There are many studies on the modification of salad dressing formulations to replace egg yolk and saturated fats. The present study describes new formulation of salad dressing with olive oil and apple vinegar to produce a functional food product...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101466/characterization-of-the-effect-of-drug-drug-interaction-on-protein-binding-in-concurrent-administration-of-sulfamethoxazol-and-diclofenac-sodium-using-bovine-serum-albumin
#8
Md Kamal Hossain, Amina Khatun, Mahmudur Rahman, Md Nahid Akter, Sadia Afreen Chowdhury, Sm Mahbubul Alam
Purpose: This project was aimed to determine the effect of concurrent administration of sulfamethoxazole and diclofenac sodium. Methods: Equilibrium dialysis method was adopted to study different protein binding aspects of sulfamethoxazole and diclofenac sodium. Results: Sulfamethoxazole showed two types of association constants; high affinity constant 29.0±0.20×10(6) M(-1) with lower number of binding sites of 0.7±1 and low affinity constant 1.13±0.20×10(6) M(-1) with higher number of binding sites of 3...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101465/nanostructured-lipid-carrier-for-topical-application-of-n-acetyl-glucosamine
#9
Lavin Aliasgharlou, Saeed Ghanbarzadeh, Hamideh Azimi, Mohammad Hossein Zarrintan, Hamed Hamishehkar
: Purpose: Hyperpigmentation occurs when melanin is overproduced in certain spots on the skin and is one of the most challenging skin conditions to treat. Although it is usually harmless, for cosmetic reasons, it is dreadfully bothersome to those who undergo it. It was reported that N-acetyl-glucosamine (NAGA) prevents melanin synthesis and alters the expression of numerous genes related to pigmentation. In spite of these advantages, NAGA cannot be employed in topical formulations due to its extremely polar characteristics...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101464/anti-inflammatory-and-antibothropic-properties-of-jatropha-elliptica-a-plant-from-brazilian-cerrado-biome
#10
Sára Cósta Ferreira-Rodrigues, Cássio Milhomens Rodrigues, Marcio Galdino Dos Santos, Jean Antonio Abraham Gautuz, Magali Glauzer Silva, José Carlos Cogo, Camila Batista-Silva, Cleiton Pita Dos Santos, Francisco Carlos Groppo, Karina Cogo-Müller, Yoko Oshima-Franco
Purpose: The aim of this study was to evaluate the antibothropic and anti-inflammatory properties of J. elliptica.Methods: Phytochemical screening and thin-layer chromatography (TLC) assays were performed on J. elliptica hydroalcoholic extract (TE) in order to observe its main constituents. The antibothropic activity of TE was evaluated by the in vitro neuromuscular blockade caused by Bothrops jararacussu venom (Bjssu), in a mouse phrenic nerve-diaphragm model (PND). A quantitative histological study was carried out to observe a possible protection of TE against the venom myotoxicity...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101463/development-of-a-novel-human-single-chain-antibody-against-egfrviii-antigen-by-phage-display-technology
#11
Leila Rahbarnia, Safar Farajnia, Hossein Babaei, Jafar Majidi, Bahman Akbari, Shiva Ahdi Khosroshahi
Purpose: EGFRvIII as the most common mutant variant of the epidermal growth factor receptor is resulting from deletion of exons 2-7 in the coding sequence and junction of exons 1 and 8 through a novel glycine residue. EGFRvIII is highly expressed in glioblastoma, carcinoma of the breast, ovary, and lung but not in normal cells. The aim of the present study was identification of a novel single chain antibody against EGFRvIII as a promising target for cancer therapy. Methods: In this study, a synthetic peptide corresponding to EGFRvIII protein was used for screening a naive human scFv phage library...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101462/identification-and-molecular-characterization-of-genes-coding-pharmaceutically-important-enzymes-from-halo-thermo-tolerant-bacillus
#12
Azam Safary, Rezvan Moniri, Maryam Hamzeh-Mivehroud, Siavoush Dastmalchi
Purpose: Robust pharmaceutical and industrial enzymes from extremophile microorganisms are main source of enzymes with tremendous stability under harsh conditions which make them potential tools for commercial and biotechnological applications. Methods: The genome of a Gram-positive halo-thermotolerant Bacillus sp. SL1, new isolate from Saline Lake, was investigated for the presence of genes coding for potentially pharmaceutical enzymes. We determined gene sequences for the enzymes laccase (CotA), l-asparaginase (ansA3, ansA1), glutamate-specific endopeptidase (blaSE), l-arabinose isomerase (araA2), endo-1,4-β mannosidase (gmuG), glutaminase (glsA), pectate lyase (pelA), cellulase (bglC1), aldehyde dehydrogenase (ycbD) and allantoinases (pucH) in the genome of Bacillus sp...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101461/acute-and-subchronic-toxicity-study-of-the-median-septum-of-juglans-regia-in-wistar-rats
#13
Asma Ravanbakhsh, Majid Mahdavi, Ghader Jalilzade-Amin, Shahram Javadi, Masoud Maham, Daryosh Mohammadnejad, Mohammad Reza Rashidi
Purpose: Median septum of Juglans regia L. (Juglandaceae) with anti-diabetic effects has been used in Iranian traditional medicine. The present study estimates both oral acute and subchronic toxicities. Methods: In the oral acute toxicity study, female Wistar rats were treated with doses of 10, 100, 1000, 1600, 2900 and 5000 mg/ kg of the Juglans regia septum of methanol extract (JRSME), and were monitored for 14 days. In subchronic study, JRSME was administered by gavage at dose of 1000 mg/kg daily in Wistar rats for 28 days...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101460/captopril-and-valsartan-may-improve-cognitive-function-through-potentiation-of-the-brain-antioxidant-defense-system-and-attenuation-of-oxidative-nitrosative-damage-in-stz-induced-dementia-in-rat
#14
Yasaman Arjmand Abbassi, Mohammad Taghi Mohammadi, Mahsa Sarami Foroshani, Javad Raouf Sarshoori
Purpose: Previous findings have shown the crucial roles of brain renin-angiotensin system (RAS) in pathogenesis of Alzheimer's disease (AD). Since RAS inhibitors may have beneficial effects on dementia and cognitive function in elderly people, the aim of present study was to examine the neuroprotective actions of captopril and valsartan on memory function and neuronal damage in experimental model of AD. Methods: Adult forty male Wistar rats (220-280g) were randomly divided into 5 groups; Control, Vehicle, Alzheimer and treatment groups...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101459/venlafaxine-induced-cytotoxicity-towards-isolated-rat-hepatocytes-involves-oxidative-stress-and-mitochondrial-lysosomal-dysfunction
#15
Elham Ahmadian, Hossein Babaei, Alireza Mohajjel Nayebi, Aziz Eftekhari, Mohammad Ali Eghbal
Purpose: Depression is a public disorder worldwide. Despite the widespread use of venlafaxine in the treatment of depression, it has been associated with the incidence of toxicities. Hence, the goal of the current investigation was to evaluate the mechanisms of venlafaxine-induced cell death in the model of the freshly isolated rat hepatocytes. Methods: Collagenase-perfused rat hepatocytes were treated with venlafaxine and other agents. Cell damage, reactive oxygen species (ROS) formation, lipid peroxidation, mitochondrial membrane potential decline, lysosomal damage, glutathione (GSH) level were analyzed...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101458/cationic-liposomes-modified-with-polyallylamine-as-a-gene-carrier-preparation-characterization-and-transfection-efficiency-evaluation
#16
Reza Kazemi Oskuee, Asma Mahmoudi, Leila Gholami, Alireza Rahmatkhah, Bizhan Malaekeh-Nikouei
Purpose: Cationic polymers and cationic liposomes have shown to be effective non-viral gene delivery vectors. In this study, we tried to improve the transfection efficiency by employing the advantages of both. Methods: For this purpose, modified polyallylamines (PAAs) were synthesized. These modifications were done through the reaction of PAA (15 KDa) with acrylate and 6-bromoalkanoic acid derivatives. Liposomes comprising of these cationic polymers and cationic lipid were prepared and extruded through polycarbonate filters to obtain desired size...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101457/therapeutic-effects-of-omega-3-fatty-acids-on-chronic-kidney-disease-associated-pruritus-a-literature-review
#17
REVIEW
Yunes Panahi, Simin Dashti-Khavidaki, Farahnoosh Farnood, Hamid Noshad, Mahsa Lotfi, Afshin Gharekhani
Uremic pruritus remains one of the most tormenting, frequent and potentially disabling problem in chronic kidney disease (CKD) patients. However, an area of substantial etiological interest with relation to uremic pruritus is the essential fatty acids deficiency. So we performed a literature review to elucidate the efficacy of omega-3 fatty acids on uremic pruritus. This review evaluated all of the studies published in English language, focusing on the clinical effects of omega-3 fatty acids on uremic pruritus...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101456/overview-of-albumin-and-its-purification-methods
#18
REVIEW
Ramin Raoufinia, Ali Mota, Neda Keyhanvar, Fatemeh Safari, Sara Shamekhi, Jalal Abdolalizadeh
As the most frequent plasma protein, albumin constitutes more than 50% of the serum proteins in healthy individuals. It has a key role in oncotic pressure maintenance and it is known as a versatile protein carrier for transportation of various endogenous and exogenous ligands. Reduced amounts of albumin in the body will lead to different kinds of diseases such as hypovolemia and hypoproteinemia. It also has various indications in shocks, burns, cardiopulmonary bypass, acute liver failure and etc. Further applications in research consist of cell culture supplement, drug delivery carrier and protein/drug stabilizer...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101455/pharmaceutical-cocrystals-regulatory-and-strategic-aspects-design-and-development
#19
REVIEW
Dipak Dilip Gadade, Sanjay Sudhakar Pekamwar
Cocrystal is a concept of the supramolecular chemistry which is gaining the extensive interest of researchers from pharmaceutical and chemical sciences and of drug regulatory agencies. The prominent reason of which is its ability to modify physicochemical properties of active pharmaceutical ingredients. During the development of the pharmaceutical product, formulators have to optimize the physicochemical properties of active pharmaceutical ingredients. Pharmaceutical cocrystals can be employed to improve vital physicochemical characteristics of a drug, including solubility, dissolution, bioavailability and stability of pharmaceutical compounds while maintaining its therapeutic activity...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101454/vitamins-are-they-safe
#20
REVIEW
Hadi Hamishehkar, Farhad Ranjdoost, Parina Asgharian, Ata Mahmoodpoor, Sarvin Sanaie
The consumption of a daily multivitamin among people all over the world is dramatically increasing in recent years. Most of the people believe that if vitamins are not effective, at least they are safe. However, the long term health consequences of vitamins consumption are unknown. This study aimed to assess the side effects and possible harmful and detrimental properties of vitamins and to discuss whether vitamins can be used as safe health products or dietary supplements. We performed a MEDLINE/PubMed, EMBASE, Scopus and Google Scholar search and assessed reference lists of the included studies which were published from 1993 through 2015...
December 2016: Advanced Pharmaceutical Bulletin
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