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International Journal of Stem Cells

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https://www.readbyqxmd.com/read/30595014/perivascular-cells-and-nadph-oxidase-inhibition-partially-restore-hyperglycemia-induced-alterations-in-hematopoietic-stem-cell-and-myeloid-derived-suppressor-cell-populations-in-the-bone-marrow
#1
Ji-Young Kim, Ji Yoon Lee, Kwon-Soo Ha, Eun-Taek Han, Won Sun Park, Chang-Ki Min, Seok-Ho Hong
Background and Objectives: Patients suffer from long-term diabetes can result in severe complications in multiple organs through induction of vascular dysfunctions. However, the effects of chronic hyperglycemic conditions on hematopoiesis and the microenvironment in the bone marrow (BM) are not yet well understood. Methods: BM cells were harvested from femurs of mice and analyzed using flow cytometry. Human PVCs were cultured in serum-free α -MEM. After 24hrs, PVC-CM was collected and filtered through a 0...
December 31, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30595009/perivascular-cells-and-nadph-oxidase-inhibition-partially-restore-hyperglycemia-induced-alterations-in-hematopoietic-stem-cell-and-myeloid-derived-suppressor-cell-populations-in-the-bone-marrow
#2
Ji-Young Kim, Ji Yoon Lee, Kwon-Soo Ha, Eun-Taek Han, Won Sun Park, Chang-Ki Min, Seok-Ho Hong
Background and Objectives: Patients suffer from long-term diabetes can result in severe complications in multiple organs through induction of vascular dysfunctions. However, the effects of chronic hyperglycemic conditions on hematopoiesis and the microenvironment in the bone marrow (BM) are not yet well understood. Methods: BM cells were harvested from femurs of mice and analyzed using flow cytometry. Human PVCs were cultured in serum-free α -MEM. After 24hrs, PVC-CM was collected and filtered through a 0...
December 31, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30595008/predictive-role-of-circulating-immune-cell-subtypes-early-after-allogeneic-hematopoietic-stem-cell-transplantation-in-patients-with-acute-leukemia
#3
Tae Woo Kim, Sung-Soo Park, Ji-Young Lim, Gi June Min, Silvia Park, Young-Woo Jeon, Seung-Ah Yahng, Seung-Hwan Shin, Sung-Eun Lee, Jae-Ho Yoon, Byung-Sik Cho, Ki-Seong Eom, Seok Lee, Hee-Je Kim, Chang-Ki Min
Background and Objectives: Cells of innate immunity normally recover in the first weeks to months after allogenenic hematopoietic stem cell transplantation (allo-HSCT). Their relevance in terms of graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect is largely unknown. The predictive role of early recovery in the immune cells on acute GVHD and GVL effect after allo-HSCT was investigated in patients with acute leukemia who achieved the first complete remission. Methods: Peripheral blood samples were taken at the median of 14 days (range, 12∼29 days) after allo-HSCT...
December 31, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30595007/effects-of-human-placental-amnion-derived-mesenchymal-stem-cells-on-proliferation-and-apoptosis-mechanisms-in-chronic-kidney-disease-in-the-rat
#4
Busra Cetinkaya, Gozde Unek, Dijle Kipmen-Korgun, Sadi Koksoy, Emin Turkay Korgun
Background and Objectives: The feature of chronic kidney failure (CKF) is loss of kidney functions due to erosion of healthy tissue and fibrosis. Recent studies showed that Mesenchymal stem cells (MSCs) differentiated into tubular epithelial cells thus renal function and structures renewed.Furthermore, MSCs protect renal function in CKF. Therefore, we aimed to investigate whether human amnion-derived mesenchymal stem cells (hAMSCs) can repair fibrosis and determine the effects on proliferation and apoptosis mechanisms in chronic kidney failure...
December 31, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30595006/effects-of-tgf-%C3%AE-1-overexpression-on-biological-characteristics-of-human-dental-pulp-derived-mesenchymal-stromal-cells
#5
Hasan Salkın, Zeynep Burçin Gönen, Ergül Ergen, Dilek Bahar, Mustafa Çetin
Objective: The aim of our study was to investigate the effect of Transforming growth factor beta-1 (TGF- β 1) gene therapy on the surface markers, multilineage differentiation, viability, apoptosis, cell cycle, DNA damage and senescence of human Dental Pulp-derived Mesenchymal Stromal Cells (hDPSC). Methods: hDPSCs were isolated from human teeth, and were cultured with 20% Fetal Bovine Serum (FBS) in minimum essential media-alpha ( α -MEM). TGF- β 1 gene transfer into hDPSCs was performed by electroporation method after the plasmid was prepared...
December 31, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30595005/mesenchymal-stem-cells-decrease-tunnel-widening-of-anterior-cruciate-ligament-reconstruction-in-rabbit-model
#6
Chang-Ich Hur, Hyeon-Wook Ahn, Jong-Keun Seon, Eun-Kyoo Song, Ga-Eon Kim
Background and Objectives: The study investigated the effect of mesenchymal stem cells (MSCs) or fibrin glue on tunnel widening after anterior cruciate ligament (ACL) reconstruction compared with biologic free control without any biologic agents in the rabbit model. Methods and Results: ACL reconstructions were performed in 18 New Zealand white rabbits. All animals were divided into 3 groups according to the following reconstruction conditions and euthanized 12 weeks postoperatively for radiologic and histologic analyses...
December 31, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30497130/mbd3-promotes-reprogramming-of-primary-human-fibroblasts
#7
Sajjida Jaffer, Pollyanna Goh, Mahnaz Abbasian, Amit C Nathwani
Mbd3 (Methyl-CpG binding domain protein), a core member of NuRD (nucleosome remodelling and deacetylation) is essential for embryogenesis. However, its role in reprogramming of somatic cells into induced pluripotent stem cells (iPSC) remains controversial. Some reports suggest that Mbd3 inhibits pluripotency, whilst others show that it greatly enhances reprogramming efficiency. Our study is the first to assess the role of Mbd3 on reprogramming of primary human fibroblasts using Yamanaka episomal plasmids (Reprogramming factors (RF) under feeder-free conditions...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30497129/rbmscs-itga5b1-promotes-human-vascular-smooth-muscle-cell-differentiation-via-enhancing-nitric-oxide-production
#8
Yingxin Zhang, Jie Ding, Cong Xu, Hongli Yang, Peng Xia, Shengjun Ma, Haiying Chen
Background and Objectives: Previous studies have shown that integrins alpha5beta1 (ITGA5B1) gene-modified rat bone marrow mesenchymal stem cells (rBMSCs) could prevent cell anoikis and increase the nitric oxide (NO) production. Here we examined the capability of rBMSCs/ITGA5B1 on the phenotype modulation of Human Pulmonary Artery Smooth Muscle Cell (HPASMC) in vitro. Methods and Results: The synthetic (dedifferentiated) phenotype of HPASMC was induced by monocrotaline (MCT, 1 μ M) for 24 h and then co-cultured with rBMSCs/ITGA5B1 in a transwell culture system...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30497128/hypoxic-preconditioned-mesenchymal-stromal-cell-therapy-in-a-rat-model-of-renal-ischemia-reperfusion-injury-development-of-optimal-protocol-to-potentiate-therapeutic-efficacy
#9
Myoung Jin Jang, Dalsan You, Jin Young Park, Kyung Kim, Joomin Aum, Chunwoo Lee, Geehyun Song, Ha Chul Shin, Nayoung Suh, Yong Man Kim, Choung-Soo Kim
Although previous and ongoing clinical studies have used stromal cells during renal ischemia-reperfusion injury (IRI), there is little consensus regarding the optimal protocol. We aimed to optimize the protocol for hypoxic preconditioned human bone marrow-derived mesenchymal stromal cell (HP-hBMSC) therapy in a rat model of renal IRI. We determined the optimal injection route (renal arterial, renal parenchymal, and tail venous injection), dose (low-dose: 1×106 , moderate-dose: 2×106 , and high-dose: 4×106 ), and injection period (pre-, concurrent-, and post-IRI)...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30408408/olig2-expressing-mesenchymal-stem-cells-enhance-functional-recovery-after-contusive-spinal-cord-injury
#10
Hwan-Woo Park, Soonyi Oh, Kyung Hee Lee, Bae Hwan Lee, Mi-Sook Chang
Background and Objectives: Glial scarring and inflammation after spinal cord injury (SCI) interfere with neural regeneration and functional recovery due to the inhibitory microenvironment of the injured spinal cord. Stem cell transplantation can improve functional recovery in experimental models of SCI, but many obstacles to clinical application remain due to concerns regarding the effectiveness and safety of stem cell transplantation for SCI patients. In this study, we investigated the effects of transplantation of human mesenchymal stem cells (hMSCs) that were genetically modified to express Olig2 in a rat model of SCI...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30343551/mir-9-controls-chemotactic-activity-of-cord-blood-cd34%C3%A2-%C2%BA-cells-by-repressing-cxcr4-expression
#11
Tae Won Ha, Hyun Soo Kang, Tae-Hee Kim, Ji Hyun Kwon, Hyun Kyu Kim, Aeli Ryu, Hyeji Jeon, Jaeseok Han, Hal E Broxmeyer, Yongsung Hwang, Yun Kyung Lee, Man Ryul Lee
Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34+ cells as well as in 293T and TF-1 cell lines...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30343550/the-pharmacological-inhibition-of-erk5-enhances-apoptosis-in-acute-myeloid-leukemia-cells
#12
Changhee Kang, Jong Soo Kim, C-Yoon Kim, Eun-Young Kim, Hyung-Min Chung
Acute myeloid leukemia (AML) is a fatal hematological malignancy which is resistant to a variety of chemotherapy drugs. Extracellular signal-regulated kinase 5 (ERK5) plays a novel role in chemoresistance in some cancer cells and this pathway is a central mediator of cell survival and apoptotic regulation. The aim of this study was to investigate the effect of ERK5 inhibitor, XMD8-92, on proliferation and apoptosis in AML cell lines. Findings showed that XMD8-92 inhibited the activation of ERK5 by G-CSF and decreased the expression of c-Myc and Cyclin D1...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30343549/immunomodulatory-effects-of-placenta-derived-mesenchymal-stem-cells-on-t-cells-by-regulation-of-foxp3-expression
#13
Soo-Hwan Kim, Jieun Jung, Kyung Jin Cho, Jong-Ho Choi, Hyeong Seon Lee, Gi Jin Kim, Seung Gwan Lee
The immunomodulatory effects of mesenchymal stem cells (MSCs) are an important mediator of their therapeutic effects in stem cell therapy and regenerative medicine. The regulation mechanism of MSCs is orchestrated by several factors in both intrinsic and extrinsic events. Recent studies have shown that the dynamic expression of cytokines secreted from MSCs control T cell function and maturation by regulating the expression of FoxP3, which figures prominently in T cell differentiation. However, there is no evidence that placenta-derived mesenchymal stem cells (PD-MSCs) have strong immunomodulatory effects on T cell function and maturation via FoxP3 expression...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30173502/improved-transfection-efficiency-and-metabolic-activity-in-human-embryonic-stem-cell-using-non-enzymatic-method
#14
C-Yoon Kim, In-Kyu Hwang, Changhee Kang, Eun-Bin Chung, Cho-Rok Jung, Hanseul Oh, Young-Hoon Jeong, Sung-Hwan Moon, Jong Soo Kim, Ki-Sung Hong, Jae-Hak Park, Hyung-Min Chung
Human embryonic stem cells (hESCs) are pluripotent cells widely used in conventional and regenerative medicine due to their ability to self-renew, proliferate and differentiate. Recently, genetic modification of stem cells using genome editing is the most advanced technique for treating hereditary diseases. Nevertheless, the low transfection efficiency of hESCs using enzymatic methods is still limited in in vitro preclinical research. To overcome these limitations, we have developed transfection methods using non-enzymatic treatments on hESCs...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30049024/comparative-study-on-acellular-dermal-graft-versus-propylene-mesh-both-either-loaded-or-unloaded-with-bm-mscs-in-healing-of-skull-bone-defect-in-rats-histological-and-immunohistochemical-study
#15
Lobna Elkhateb, Adel Zohdy, Suzi Sobhy Atalla, Manal Hassan Moussa, Ghada Galal Hamam, Fatma Abd Elkarim Abu Zahra
Bone defect occurs as a consequence of many conditions. Diseased bones don't heal properly and defects in face area need proper bone reconstruction to avoid psychological and social problems. Tissue engineering is an emerging new modality of treatment. We thought to study different methods to fill skull bone defect in rats in order to find the most safe and effective method. So, this study was designed to evaluate the efficacy of acellular dermal graft (ADM) versus propylene mesh both either loaded or unloaded with bone marrow derived mesenchymal stem cells (BM-MSCs) in healing of skull bone defect of a 5 mm diameter...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30021253/pja2-inhibits-wnt-%C3%AE-catenin-signaling-by-reducing-the-level-of-tcf-lef1
#16
Yonghee Song, Somyung Lee, Jeong-Rae Kim, Eek-Hoon Jho
Ubiquitination of proteins plays an essential role in various cellular processes, including protein degradation, DNA repair, and cell signaling pathways. Previous studies have shown that protein ubiquitination is implicated in regulating pluripotency as well as fate determination of stem cells. To identify how protein ubiquitination affects differentiation of embryonic stem cells, we analyzed microarray data, which are available in the public domain, of E3 ligases and deubiquitinases whose levels changed during stem cell differentiation...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/30021252/effect-of-stem-cells-and-gene-transfected-stem-cells-therapy-on-the-pancreas-of-experimentally-induced-type-1-diabetes
#17
Maha Baligh Zickri, Gihan Ibrahim Aboul-Fotouh, Abeer Ibraheem Omar, Asmaa Ahmed El-Shafei, Ahmed Mahmoud Reda
Background and Objectives: Insulin secretion entirely depends on Ca2+ influx and sequestration into endoplasmic reticulum (ER) of β-cells, performed by Sarco-ER Ca2+ -ATPase 2b (SERCA2b). In diabetes, SERCA2b is decreased in the β-cells leading to impaired intracellular Ca2+ homeostasis and insulin secretion. Adipose mesenchymal stem cells (AMSCs) play a potential role in transplantation in animal models. The present study aimed at investigating and comparing the therapeutic effect of non-transfected AMSCs and SERCA2b gene transfected AMSCs on the pancreas of induced diabetes type 1 in rat...
November 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29843194/the-role-of-microenvironment-in-preserving-the-potency-of-adult-porcine-pulmonary-valve-stem-cells-in-vitro
#18
Fariba Chalajour, Arpi Siyahian, Frank L Hanley
Background and Objective: The potency of tissue resident stem cells is regulated primarily by inputs from the local microenvironment. Isolation of stem cells through enzymatic digestion of tissue may affect epigenetic regulation of cell fate and performance. Here we employ a non-enzymatic method to harvest and investigate tissue resident stem cells from the adult porcine pulmonary valve. Methods and Results: The presence of c-Kit⁺ stem cells within the valve tissue was confirmed by immunohistochemistry...
May 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29843193/large-scale-isolation-expansion-and-characterization-of-human-amniotic-epithelial-cells
#19
Sanjay Gottipamula, K N Sridhar
Background and Objectives: The human Amniotic epithelial cells (AME) derived from amniotic membrane of placenta have been considered as the potential fetal stem cell source with minimal or no ethical concerns and are important therapeutic tool for anti-fibrotic and regenerative therapies. Methods and Results: Here, we evaluated the isolation, media screening, scale-up and characterization of AME cells. The isolation, expansion of AMEs were performed by sequential passaging and growth kinetics studies...
May 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29843192/cd73-cd90-cd105-and-cadherin-11-rt-pcr-screening-for-mesenchymal-stem-cells-from-cryopreserved-human-cord-tissue
#20
Hung Pham, Richard Tonai, Miya Wu, Chiara Birtolo, Monica Chen
Background and Objectives: Mesenchymal stem cells (MSCs) are self-renewing, non-specialized cells used clinically in tissue regeneration and sourced from bone marrow, peripheral blood, umbilical cord blood and umbilical cord tissue (UCT). To demonstrate an alternative method for MSC detection, cryopreserved UCT and expanded MSC were screened for MSC markers CD73, CD90, CD105 and CDH-11 by RT-PCR. Methods and Results: Human UCT were washed, sectioned, cryopreserved with 10% DMSO and stored in the vapor phase of liquid nitrogen...
May 30, 2018: International Journal of Stem Cells
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