journal
MENU ▼
Read by QxMD icon Read
search

Stem Cell Reports

journal
https://www.readbyqxmd.com/read/28216145/a-human-neural-crest-stem-cell-derived-dopaminergic-neuronal-model-recapitulates-biochemical-abnormalities-in-gba1-mutation-carriers
#1
Shi-Yu Yang, Michelle Beavan, Kai-Yin Chau, Jan-Willem Taanman, Anthony H V Schapira
Numerically the most important risk factor for the development of Parkinson's disease (PD) is the presence of mutations in the glucocerebrosidase GBA1 gene. In vitro and in vivo studies show that GBA1 mutations reduce glucocerebrosidase (GCase) activity and are associated with increased α-synuclein levels, reflecting similar changes seen in idiopathic PD brain. We have developed a neural crest stem cell-derived dopaminergic neuronal model that recapitulates biochemical abnormalities in GBA1 mutation-associated PD...
February 15, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216148/epigenetic-modification-of-the-ccl5-ccr1-erk-axis-enhances-glioma-targeting-in-dedifferentiation-reprogrammed-bmscs
#2
Rui Chen, Wayne Yuk-Wai Lee, Xiao Hu Zhang, Jie Ting Zhang, Sien Lin, Liang Liang Xu, Biao Huang, Fu Yuan Yang, Hai Long Liu, Bin Wang, Lai Ling Tsang, Sandrine Willaime-Morawek, Gang Li, Hsiao Chang Chan, Xiaohua Jiang
The success of stem cell-mediated gene therapy in cancer treatment largely depends on the specific homing ability of stem cells. We have previously demonstrated that after in vitro induction of neuronal differentiation and dedifferentiation, bone marrow stromal cells (BMSCs) revert to a primitive stem cell population (De-neu-BMSCs) distinct from naive BMSCs. We report here that De-neu-BMSCs express significantly higher levels of chemokines, and display enhanced homing abilities to glioma, the effect of which is mediated by the activated CCL5/CCR1/ERK axis...
February 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216147/differential-responses-of-human-fetal-brain-neural-stem-cells-to-zika-virus-infection
#3
Erica L McGrath, Shannan L Rossi, Junling Gao, Steven G Widen, Auston C Grant, Tiffany J Dunn, Sasha R Azar, Christopher M Roundy, Ying Xiong, Deborah J Prusak, Bradford D Loucas, Thomas G Wood, Yongjia Yu, Ildefonso Fernández-Salas, Scott C Weaver, Nikos Vasilakis, Ping Wu
Zika virus (ZIKV) infection causes microcephaly in a subset of infants born to infected pregnant mothers. It is unknown whether human individual differences contribute to differential susceptibility of ZIKV-related neuropathology. Here, we use an Asian-lineage ZIKV strain, isolated from the 2015 Mexican outbreak (Mex1-7), to infect primary human neural stem cells (hNSCs) originally derived from three individual fetal brains. All three strains of hNSCs exhibited similar rates of Mex1-7 infection and reduced proliferation...
February 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216146/divergent-levels-of-marker-chromosomes-in-an-hipsc-based-model-of%C3%A2-psychosis
#4
Julia Tcw, Claudia M B Carvalho, Bo Yuan, Shen Gu, Alyssa N Altheimer, Shane McCarthy, Dheeraj Malhotra, Jonathan Sebat, Arthur J Siegel, Uwe Rudolph, James R Lupski, Deborah L Levy, Kristen J Brennand
In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures...
February 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28196690/gata6-plays-an-important-role-in-the-induction-of-human-definitive-endoderm-development-of-the-pancreas-and-functionality-of-pancreatic-%C3%AE-%C3%A2-cells
#5
Amita Tiyaboonchai, Fabian L Cardenas-Diaz, Lei Ying, Jean Ann Maguire, Xiuli Sim, Chintan Jobaliya, Alyssa L Gagne, Siddharth Kishore, Diana E Stanescu, Nkecha Hughes, Diva D De Leon, Deborah L French, Paul Gadue
Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expression of several different GATA family members. Using the endodermal progenitor stem cell culture system to bypass the developmental block at the endoderm stage, cell lines with mutations in one or both GATA6 alleles could be differentiated into β-like cells but with reduced efficiency...
February 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28196689/subependymal-zone-derived-oligodendroblasts-respond-to-focal-demyelination-but-fail-to-generate-myelin-in-young-and-aged-mice
#6
Ilias Kazanis, Kimberley A Evans, Evangelia Andreopoulou, Christina Dimitriou, Christos Koutsakis, Ragnhildur Thora Karadottir, Robin J M Franklin
Two populations of oligodendrogenic progenitors co-exist within the corpus callosum (CC) of the adult mouse. Local, parenchymal oligodendrocyte progenitor cells (pOPCs) and progenitors generated in the subependymal zone (SEZ) cytogenic niche. pOPCs are committed perinatally and retain their numbers through self-renewing divisions, while SEZ-derived cells are relatively "young," being constantly born from neural stem cells. We compared the behavior of these populations, labeling SEZ-derived cells using hGFAP:Cre(Ert2) mice, within the homeostatic and regenerating CC of the young-adult and aging brain...
February 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216149/direct-generation-of-human-neuronal-cells-from-adult-astrocytes-by-small-molecules
#7
Longfei Gao, Wuqiang Guan, Min Wang, Huihan Wang, Jiali Yu, Qing Liu, Binlong Qiu, Yongchun Yu, Yifang Ping, Xiuwu Bian, Li Shen, Gang Pei
Astrocytes, due to the proximity to neuronal lineage and capability to proliferate, are ideal starting cells to regenerate neurons. Human fetal astrocytes have been successfully converted into neuronal cells by small molecules, which offered a broader range of further applications than transcription factor-mediated neuronal reprogramming. Here we report that human adult astrocytes could also be converted into neuronal cells by a different set of small molecules. These induced cells exhibited typical neuronal morphologies, expressed neuronal markers, and displayed neuronal electrophysiological properties...
February 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28196692/shisa6-confers-resistance-to-differentiation-promoting-wnt-%C3%AE-catenin-signaling-in-mouse-spermatogenic-stem-cells
#8
Moe Tokue, Kanako Ikami, Seiya Mizuno, Chiyo Takagi, Asuka Miyagi, Ritsuko Takada, Chiyo Noda, Yu Kitadate, Kenshiro Hara, Hiroko Mizuguchi, Takuya Sato, Makoto Mark Taketo, Fumihiro Sugiyama, Takehiko Ogawa, Satoru Kobayashi, Naoto Ueno, Satoru Takahashi, Shinji Takada, Shosei Yoshida
In the seminiferous tubules of mouse testes, a population of glial cell line-derived neurotrophic factor family receptor alpha 1 (GFRα1)-positive spermatogonia harbors the stem cell functionality and supports continual spermatogenesis, likely independent of asymmetric division or definitive niche control. Here, we show that activation of Wnt/β-catenin signaling promotes spermatogonial differentiation and reduces the GFRα1(+) cell pool. We further discovered that SHISA6 is a cell-autonomous Wnt inhibitor that is expressed in a restricted subset of GFRα1(+) cells and confers resistance to the Wnt/β-catenin signaling...
February 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28196691/healing-of-a-large-long-bone-defect-through-serum-free-in%C3%A2-vitro-priming-of%C3%A2-human-periosteum-derived-cells
#9
Johanna Bolander, Wei Ji, Jeroen Leijten, Liliana Moreira Teixeira, Veerle Bloemen, Dennis Lambrechts, Malay Chaklader, Frank P Luyten
Clinical translation of cell-based strategies for regenerative medicine demands predictable in vivo performance where the use of sera during in vitro preparation inherently limits the efficacy and reproducibility. Here, we present a bioinspired approach by serum-free pre-conditioning of human periosteum-derived cells, followed by their assembly into microaggregates simultaneously primed with bone morphogenetic protein 2 (BMP-2). Pre-conditioning resulted in a more potent progenitor cell population, while aggregation induced osteochondrogenic differentiation, further enhanced by BMP-2 stimulation...
February 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216144/patient-ipsc-derived-neurons-for-disease-modeling-of-frontotemporal-dementia-with-mutation-in-chmp2b
#10
Yu Zhang, Benjamin Schmid, Nanett K Nikolaisen, Mikkel A Rasmussen, Blanca I Aldana, Mikkel Agger, Kirstine Calloe, Tina C Stummann, Hjalte M Larsen, Troels T Nielsen, Jinrong Huang, Fengping Xu, Xin Liu, Lars Bolund, Morten Meyer, Lasse K Bak, Helle S Waagepetersen, Yonglun Luo, Jørgen E Nielsen, Bjørn Holst, Christian Clausen, Poul Hyttel, Kristine K Freude
The truncated mutant form of the charged multivesicular body protein 2B (CHMP2B) is causative for frontotemporal dementia linked to chromosome 3 (FTD3). CHMP2B is a constituent of the endosomal sorting complex required for transport (ESCRT) and, when mutated, disrupts endosome-to-lysosome trafficking and substrate degradation. To understand the underlying molecular pathology, FTD3 patient induced pluripotent stem cells (iPSCs) were differentiated into forebrain-type cortical neurons. FTD3 neurons exhibited abnormal endosomes, as previously shown in patients...
February 6, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216143/enhanced-functional-recovery-from-spinal-cord-injury-in-aged-mice-after-stem-cell-transplantation-through-hgf-induction
#11
Morito Takano, Soya Kawabata, Shinsuke Shibata, Akimasa Yasuda, Satoshi Nori, Osahiko Tsuji, Narihito Nagoshi, Akio Iwanami, Hayao Ebise, Keisuke Horiuchi, Hideyuki Okano, Masaya Nakamura
The number of elderly patients with spinal cord injury (SCI) is increasing worldwide, representing a serious burden for both the affected patients and the community. Previous studies have demonstrated that neural stem cell (NSC) transplantation is an effective treatment for SCI in young animals. Here we show that NSC transplantation is as effective in aged mice as it is in young mice, even though aged mice exhibit more severe neurological deficits after SCI. NSCs grafted into aged mice exhibited better survival than those grafted into young mice...
February 6, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28216142/astrocytic-calcium-waves-signal-brain-injury-to-neural-stem-and%C3%A2-progenitor%C3%A2-cells
#12
Anna Kraft, Eduardo Rosales Jubal, Ruth von Laer, Claudia Döring, Adriana Rocha, Moyo Grebbin, Martin Zenke, Helmut Kettenmann, Albrecht Stroh, Stefan Momma
Brain injuries, such as stroke or trauma, induce neural stem cells in the subventricular zone (SVZ) to a neurogenic response. Very little is known about the molecular cues that signal tissue damage, even over large distances, to the SVZ. Based on our analysis of gene expression patterns in the SVZ, 48 hr after an ischemic lesion caused by middle cerebral artery occlusion, we hypothesized that the presence of an injury might be transmitted by an astrocytic traveling calcium wave rather than by diffusible factors or hypoxia...
February 3, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28111279/a-comprehensive-ethnically-diverse-library-of-sickle-cell-disease-specific-induced-pluripotent-stem-cells
#13
Seonmi Park, Andreia Gianotti-Sommer, Francisco Javier Molina-Estevez, Kim Vanuytsel, Nick Skvir, Amy Leung, Sarah S Rozelle, Elmutaz Mohammed Shaikho, Isabelle Weir, Zhihua Jiang, Hong-Yuan Luo, David H K Chui, Maria Stella Figueiredo, Abdulraham Alsultan, Amein Al-Ali, Paola Sebastiani, Martin H Steinberg, Gustavo Mostoslavsky, George J Murphy
Sickle cell anemia affects millions of people worldwide and is an emerging global health burden. As part of a large NIH-funded NextGen Consortium, we generated a diverse, comprehensive, and fully characterized library of sickle-cell-disease-specific induced pluripotent stem cells (iPSCs) from patients of different ethnicities, β-globin gene (HBB) haplotypes, and fetal hemoglobin (HbF) levels. iPSCs stand to revolutionize the way we study human development, model disease, and perhaps eventually, treat patients...
January 18, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28199830/ipsc-derived-retina-transplants-improve-vision-in-rd1-end-stage-retinal-degeneration-mice
#14
Michiko Mandai, Momo Fujii, Tomoyo Hashiguchi, Genshiro A Sunagawa, Shin-Ichiro Ito, Jianan Sun, Jun Kaneko, Junki Sho, Chikako Yamada, Masayo Takahashi
No abstract text is available yet for this article.
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28199829/preclinical-efficacy-failure-of-human-cns-derived-stem-cells-for-use-in-the-pathway-study-of%C3%A2-cervical-spinal-cord-injury
#15
Aileen J Anderson, Katja M Piltti, Mitra J Hooshmand, Rebecca A Nishi, Brian J Cummings
We previously showed the efficacy of multiple research cell lines (RCLs) of human CNS neural stem cells (HuCNS-SCs) in mouse and rat models of thoracic spinal cord injury (SCI), supporting a thoracic SCI clinical trial. Experts recommend in vivo preclinical testing of the intended clinical cell lot/line (CCL) in models with validity for the planned clinical target. We therefore tested the efficacy of two HuCNS-SC lines in cervical SCI: one RCL, and one CCL intended for use in the Pathway Study of cervical SCI in man...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28199828/hucns-sc-human-nscs-fail-to-differentiate-form-ectopic-clusters-and-provide-no-cognitive-benefits-in-a-transgenic-model-of-alzheimer-s-disease
#16
Samuel E Marsh, Stephen T Yeung, Maria Torres, Lydia Lau, Joy L Davis, Edwin S Monuki, Wayne W Poon, Mathew Blurton-Jones
Transplantation of neural stem cells (NSCs) can improve cognition in animal models of Alzheimer's disease (AD). However, AD is a protracted disorder, and prior studies have examined only short-term effects. We therefore used an immune-deficient model of AD (Rag-5xfAD mice) to examine long-term transplantation of human NSCs (StemCells Inc.; HuCNS-SCs). Five months after transplantation, HuCNS-SCs had engrafted and migrated throughout the hippocampus and exhibited no differences in survival or migration in response to β-amyloid pathology...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28199827/response-to-stemcells-inc
#17
LETTER
Edwin S Monuki, Aileen J Anderson, Mathew Blurton-Jones, Brian J Cummings
No abstract text is available yet for this article.
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28199826/reaction-from-stemcells-inc-to-two-papers-in-stem-cell-reports-on-the-efficacy-of-human-nscs-in-mouse-models-of-alzheimer-s-disease-and-spinal-cord-injury
#18
LETTER
(no author information available yet)
No abstract text is available yet for this article.
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28199825/lessons-learned-from-pioneering-neural-stem-cell-studies
#19
Sally Temple, Lorenz Studer
As stem cell products are increasingly entering early stage clinical trials, we are learning from experience about how cell products may be best assessed for safety and efficacy. In two papers published in this issue of Stem Cell Reports, a human neural stem cell product, HuCNS-SC, failed to demonstrate efficacy in central nervous system repair in two different animal models (Anderson et al., 2017; Marsh et al., 2017), although closely related research-grade cell products showed evidence of efficacy. This indicates the need for increased cell characterization to determine comparability of lots proposed for pre-clinical and clinical use...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28162998/common-telomere-changes-during-in%C3%A2-vivo-reprogramming-and-early-stages-of-tumorigenesis
#20
Rosa M Marión, Isabel López de Silanes, Lluc Mosteiro, Benjamin Gamache, María Abad, Carmen Guerra, Diego Megías, Manuel Serrano, Maria A Blasco
Reprogramming of differentiated cells into induced pluripotent stem cells has been recently achieved in vivo in mice. Telomeres are essential for chromosomal stability and determine organismal life span as well as cancer growth. Here, we study whether tissue dedifferentiation induced by in vivo reprogramming involves changes at telomeres. We find telomerase-dependent telomere elongation in the reprogrammed areas. Notably, we found highly upregulated expression of the TRF1 telomere protein in the reprogrammed areas, which was independent of telomere length...
February 14, 2017: Stem Cell Reports
journal
journal
47770
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"