journal
https://read.qxmd.com/read/38193375/a-beginner-s-guide-to-study-extracellular-vesicles-in-human-blood-plasma-and-serum
#21
REVIEW
Rienk Nieuwland, Pia R-M Siljander
Blood is the most commonly used body fluid for obtaining and studying extracellular vesicles (EVs). While blood is a standard choice for clinical analysis, using blood as a source of EVs introduces multiple layers of complexity. At the Blood Extracellular Vesicle Workshop organized by the International Society for Extracellular Vesicles in Helsinki (2022), it became evident that beginner researchers lack trustworthy information on how to initiate their research and avoid common pitfalls. This educational guide explains the composition and frequently used terminology of blood, provides guidelines for blood collection, and the preparation of plasma and serum...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38191961/novel-method-for-collecting-hippocampal-interstitial-fluid-extracellular-vesicles-ev-isf-reveals-sex-dependent-changes-in-microglial-ev-proteome-in-response-to-a%C3%AE-pathology
#22
JOURNAL ARTICLE
Morgan C Pait, Sarah D Kaye, Yixin Su, Ashish Kumar, Sangeeta Singh, Stephen C Gironda, Samantha Vincent, Maria Anwar, Caitlin M Carroll, James Andy Snipes, Jingyun Lee, Cristina M Furdui, Gagan Deep, Shannon L Macauley
Brain-derived extracellular vesicles (EVs) play an active role in Alzheimer's disease (AD), relaying important physiological information about their host tissues. The internal cargo of EVs is protected from degradation, making EVs attractive AD biomarkers. However, it is unclear how circulating EVs relate to EVs isolated from disease-vulnerable brain regions. We developed a novel method for collecting EVs from the hippocampal interstitial fluid (ISF) of live mice. EVs (EVISF ) were isolated via ultracentrifugation and characterized by nanoparticle tracking analysis, immunogold labelling, and flow cytometry...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38191764/extracellular-vesicle-mediated-delivery-of-crispr-cas9-ribonucleoprotein-complex-targeting-proprotein-convertase-subtilisin-kexin-type-9-pcsk9-in-primary-mouse-hepatocytes
#23
JOURNAL ARTICLE
Nazma F Ilahibaks, Thomas A Kluiver, Olivier G de Jong, Saskia C A de Jager, Raymond M Schiffelers, Pieter Vader, Weng Chuan Peng, Zhiyong Lei, Joost P G Sluijter
The loss-of-function of the proprotein convertase subtilisin-kexin type 9 (Pcsk9) gene has been associated with significant reductions in plasma serum low-density lipoprotein cholesterol (LDL-C) levels. Both CRISPR/Cas9 and CRISPR-based editor-mediated Pcsk9 inactivation have successfully lowered plasma LDL-C and PCSK9 levels in preclinical models. Despite the promising preclinical results, these studies did not report how vehicle-mediated CRISPR delivery inactivating Pcsk9 affected low-density lipoprotein receptor recycling in vitro or ex vivo...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38179654/size-matters-functional-differences-of-small-extracellular-vesicle-subpopulations-in-cardiac-repair-responses
#24
JOURNAL ARTICLE
Simonides Immanuel van de Wakker, Julia Bauzá-Martinez, Carla Ríos Arceo, Herak Manjikian, Christian Jamie Bernard Snijders Blok, Marieke Theodora Roefs, Eduard Willms, Renee Goverdina Catharina Maas, Matti Feije Pronker, Olivier Gerrit de Jong, Wei Wu, André Görgens, Samir El Andaloussi, Joost Petrus Gerardus Sluijter, Pieter Vader
Cardiac progenitor cell (CPC)-derived small extracellular vesicles (sEVs) exhibit great potential to stimulate cardiac repair. However, the multifaceted nature of sEV heterogeneity presents a challenge in understanding the distinct mechanisms underlying their regenerative abilities. Here, a dual-step multimodal flowthrough and size-exclusion chromatography method was applied to isolate and separate CPC-derived sEV subpopulations to study the functional differences related to cardiac repair responses. Three distinct sEV subpopulations were identified with unique protein profiles...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38173127/dysregulation-of-extracellular-vesicle-protein-cargo-in-female-myalgic-encephalomyelitis-chronic-fatigue-syndrome-cases-and-sedentary-controls-in-response-to-maximal-exercise
#25
JOURNAL ARTICLE
Ludovic Giloteaux, Katherine A Glass, Arnaud Germain, Carl J Franconi, Sheng Zhang, Maureen R Hanson
In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue and reduces the risk of chronic diseases. Although exercise is suggested as a lifestyle intervention to manage various chronic illnesses, it negatively affects people with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), who suffer from exercise intolerance. We hypothesized that altered extracellular vesicle (EV) signalling in ME/CFS patients after an exercise challenge may contribute to their prolonged and exacerbated negative response to exertion (post-exertional malaise)...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38158550/recommendations-for-reproducibility-of-cerebrospinal-fluid-extracellular-vesicle-studies
#26
JOURNAL ARTICLE
Ursula S Sandau, Setty M Magaña, Júlia Costa, John P Nolan, Tsuneya Ikezu, Laura J Vella, Hannah K Jackson, Lissette Retana Moreira, Paola Loreto Palacio, Andrew F Hill, Joseph F Quinn, Kendall R Van Keuren-Jensen, Trevor J McFarland, Joanna Palade, Eric A Sribnick, Huaqi Su, Kostas Vekrellis, Beth Coyle, You Yang, Juan M Falcón-Perez, Rienk Nieuwland, Julie A Saugstad
Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38151475/high-fat-diets-you-are-what-you-eat%C3%A2-your-extracellular-vesicles-too
#27
REVIEW
Sophie Rome, Stefano Tacconi
Recent works indicate that the lipid composition of extracellular vesicles (EVs) can modify their biological functions and their incorporation into recipient cells. In particular high-fat diets affect EV biogenesis, EV lipid composition, EV targeting and consequently the cross-talk between tissues. This review connects different research topics to show that a vicious circle is established during the development of high-fat diet-induced obesity, connecting the alteration of lipid metabolism, the composition of extracellular vesicles and the spread of deleterious lipids between tissues, which participates in NAFLD/NASH and diabetes development...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38151470/extracellular-vesicles-from-organoid-derived-human-retinal-progenitor-cells-prevent-lipid-overload-induced-retinal-pigment-epithelium-injury-by-regulating-fatty-acid-metabolism
#28
JOURNAL ARTICLE
Hui Gao, Yuxiao Zeng, Xiaona Huang, Luodan A, Qingle Liang, Jing Xie, Xi Lin, Jing Gong, Xiaotang Fan, Ting Zou, Haiwei Xu
Retinal degeneration (RD), a group of diseases leading to irreversible vision loss, is characterised by retinal pigment epithelium (RPE) or retinal neuron damage and loss. With fewer risks of immune rejection and tumorigenesis, stem cell-secreted extracellular vesicles (EVs) offer a new cell-free therapeutic paradigm for RD, which remains to be investigated. Human retinal organoid-derived retinal progenitor cells (hERO-RPCs) are an easily accessible and advanced cell source for RD treatment. However, hERO-RPCs-derived EVs require further characterisation...
January 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38124271/uptake-of-extracellular-vesicles-into-immune-cells-is-enhanced-by-the-protein-corona
#29
JOURNAL ARTICLE
Laura Dietz, Jennifer Oberländer, Ana Mateos-Maroto, Jenny Schunke, Michael Fichter, Eva-Maria Krämer-Albers, Katharina Landfester, Volker Mailänder
The influence of a protein corona on the uptake of nanoparticles in cells has been demonstrated in various publications over the last years. Extracellular vesicles (EVs), can be seen as natural nanoparticles. However, EVs are produced under different cell culture conditions and little is known about the protein corona forming on EVs and its influence on their uptake by target cells. Here, we use a proteomic approach in order to analyze the protein composition of the EVs themselves and the protein composition of a human blood plasma protein corona around EVs...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38124258/extracellular-vesicle-associated-cholesterol-supports-the-regenerative-functions-of-macrophages-in-the-brain
#30
JOURNAL ARTICLE
Sam Vanherle, Jeroen Guns, Melanie Loix, Fleur Mingneau, Tess Dierckx, Flore Wouters, Koen Kuipers, Tim Vangansewinkel, Esther Wolfs, Paula Pincela Lins, Annelies Bronckaers, Ivo Lambrichts, Jonas Dehairs, Johannes V Swinnen, Sanne G S Verberk, Mansour Haidar, Jerome J A Hendriks, Jeroen F J Bogie
Macrophages play major roles in the pathophysiology of various neurological disorders, being involved in seemingly opposing processes such as lesion progression and resolution. Yet, the molecular mechanisms that drive their harmful and benign effector functions remain poorly understood. Here, we demonstrate that extracellular vesicles (EVs) secreted by repair-associated macrophages (RAMs) enhance remyelination ex vivo and in vivo by promoting the differentiation of oligodendrocyte precursor cells (OPCs). Guided by lipidomic analysis and applying cholesterol depletion and enrichment strategies, we find that EVs released by RAMs show markedly elevated cholesterol levels and that cholesterol abundance controls their reparative impact on OPC maturation and remyelination...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38117000/extracellular-vesicles-derived-from-nasopharyngeal-carcinoma-induce-the-emergence-of-mature-regulatory-dendritic-cells-using-a-galectin-9-dependent-mechanism
#31
JOURNAL ARTICLE
Anthony Lefebvre, Camille Trioën, Sarah Renaud, William Laine, Benjamin Hennart, Clément Bouchez, Bertrand Leroux, Delphine Allorge, Jérôme Kluza, Elisabeth Werkmeister, Guillaume Paul Grolez, Nadira Delhem, Olivier Moralès
Nasopharyngeal carcinoma-derived small extracellular vesicles (NPCSEVs) have an immunosuppressive impact on the tumour microenvironment. In this study, we investigated their influence on the generation of tolerogenic dendritic cells and the potential involvement of the galectin-9 (Gal9) they carry in this process. We analysed the phenotype and immunosuppressive properties of NPCSEVs and explored the ability of DCs exposed to NPCSEVs (NPCSEV-DCs) to regulate T cell proliferation. To assess their impact at the pathophysiological level, we performed real-time fluorescent chemoattraction assays...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38082562/multiomic-integration-reveals-neuronal-extracellular-vesicle-coordination-of-gliotic-responses-in-degeneration
#32
JOURNAL ARTICLE
Adrian V Cioanca, Yvette Wooff, Riemke Aggio-Bruce, Rakshanya Sekar, Catherine Dietrich, Riccardo Natoli
In the central nervous system (CNS), including in the retina, neuronal-to-glial communication is critical for maintaining tissue homeostasis including signal transmission, transfer of trophic factors, and in the modulation of inflammation. Extracellular vesicle (EV)-mediated transport of molecular messages to regulate these processes has been suggested as a mechanism by which bidirectional communication between neuronal and glial cells can occur. In this work we employed multiomics integration to investigate the role of EV communication pathways from neurons to glial cells within the CNS, using the mouse retina as a readily accessible representative CNS tissue...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38082559/proteomic-comparison-between-non-purified-cerebrospinal-fluid-and-cerebrospinal-fluid-derived-extracellular-vesicles-from-patients-with-alzheimer-s-parkinson-s-and-lewy-body-dementia
#33
JOURNAL ARTICLE
Yael Hirschberg, Natalia Valle-Tamayo, Oriol Dols-Icardo, Sebastiaan Engelborghs, Bart Buelens, Roosmarijn E Vandenbroucke, Yannick Vermeiren, Kurt Boonen, Inge Mertens
Dementia is a leading cause of death worldwide, with increasing prevalence as global life expectancy increases. The most common neurodegenerative disorders are Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD). With this study, we took an in-depth look at the proteome of the (non-purified) cerebrospinal fluid (CSF) and the CSF-derived extracellular vesicles (EVs) of AD, PD, PD-MCI (Parkinson's disease with mild cognitive impairment), PDD and DLB patients analysed by label-free mass spectrometry...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38072803/metabolic-labelling-of-a-subpopulation-of-small-extracellular-vesicles-using-a-fluorescent-palmitic-acid-analogue
#34
JOURNAL ARTICLE
Valeria Barreca, Zaira Boussadia, Deborah Polignano, Lorenzo Galli, Valentina Tirelli, Massimo Sanchez, Mario Falchi, Lucia Bertuccini, Francesca Iosi, Massimo Tatti, Massimo Sargiacomo, Maria Luisa Fiani
Exosomes are among the most puzzling vehicles of intercellular communication, but several crucial aspects of their biogenesis remain elusive, primarily due to the difficulty in purifying vesicles with similar sizes and densities. Here we report an effective methodology for labelling small extracellular vesicles (sEV) using Bodipy FL C16, a fluorescent palmitic acid analogue. In this study, we present compelling evidence that the fluorescent sEV population derived from Bodipy C16-labelled cells represents a discrete subpopulation of small exosomes following an intracellular pathway...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38063210/miblood-ev-minimal-information-to-enhance-the-quality-and-reproducibility-of-blood-extracellular-vesicle-research
#35
JOURNAL ARTICLE
Fabrice Lucien, Dakota Gustafson, Metka Lenassi, Bo Li, Jacob J Teske, Eric Boilard, Katharina Clemm von Hohenberg, Juan Manual Falcón-Perez, Alice Gualerzi, Antonia Reale, Jennifer C Jones, Cecilia Lässer, Charlotte Lawson, Irina Nazarenko, Lorraine O'Driscoll, Ryan Pink, Pia R-M Siljander, Carolina Soekmadji, An Hendrix, Joshua A Welsh, Kenneth W Witwer, Rienk Nieuwland
Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38054534/a-clinically-relevant-large-scale-biomanufacturing-workflow-to-produce-natural-killer-cells-and-natural-killer-cell-derived-extracellular-vesicles-for-cancer-immunotherapy
#36
JOURNAL ARTICLE
Frederic St-Denis-Bissonnette, Sarah E Cummings, Shirley Qiu, Andrew Stalker, Gauri Muradia, Jelica Mehic, Karan Mediratta, Shelby Kaczmarek, Dylan Burger, Seung-Hwan Lee, Lisheng Wang, Jessie R Lavoie
Natural killer cell-derived extracellular vesicles (NK-EVs) have shown promising potential as biotherapeutics for cancer due to their unique attributes as cytotoxic nanovesicles against cancer cells and immune-modulatory activity towards immune cells. However, a biomanufacturing workflow is needed to produce clinical-grade NK-EVs for pre-clinical and clinical applications. This study established a novel biomanufacturing workflow using a closed-loop hollow-fibre bioreactor to continuously produce NK-EVs from the clinically relevant NK92-MI cell line under serum-free, Xeno-free and feeder-free conditions following GMP-compliant conditions...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38050834/single-particle-analysis-of-circulating-bacterial-extracellular-vesicles-reveals-their-biogenesis-changes-in-blood-and-links-to-intestinal-barrier
#37
JOURNAL ARTICLE
Zihao Ou, Bo Situ, Xinyue Huang, Yicong Xue, Xiaojing He, Qianbei Li, Dejin Ou, Bairong He, Jing Chen, Yiyao Huang, Lulu Deng, Minying Zhang, Qian Wang, Lei Zheng
Bacterial extracellular vesicles (BEVs) are nano-size particles secreted by bacteria that carry various bioactive components. These vesicles are thought to provide a new window into the mechanisms by which bacteria affect their hosts, but their fundamental proprieties within human remain poorly understood. Here, we developed a single-vesicle analytical platform that enabled BEV detection in complex biological samples of host. Using this platform, we found the presence of BEVs in the host circulation and they were mainly derived from gut microbes...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38050832/exploring-the-role-of-polymers-to-overcome-ongoing-challenges-in-the-field-of-extracellular-vesicles
#38
REVIEW
Laurianne Simon, Vincent Lapinte, Marie Morille
Extracellular vesicles (EVs) are naturally occurring nanoparticles released from all eucaryotic and procaryotic cells. While their role was formerly largely underestimated, EVs are now clearly established as key mediators of intercellular communication. Therefore, these vesicles constitute an attractive topic of study for both basic and applied research with great potential, for example, as a new class of biomarkers, as cell-free therapeutics or as drug delivery systems. However, the complexity and biological origin of EVs sometimes complicate their identification and therapeutic use...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38050829/correlation-between-membrane-proteins-and-sizes-of-extracellular-vesicles-and-particles-a-potential-signature-for-cancer-diagnosis
#39
JOURNAL ARTICLE
Chunhui Zhai, Feng Xie, Jiaying Xu, Yuting Yang, Weiqiang Zheng, Haiyan Hu, Xianting Ding, Hui Yu
Extracellular vesicles and particles (EVPs) are recognized as ideal liquid biopsy tools for cancer detection, and membrane proteins are commonly used EVP biomarkers. However, bulk analysis of EVP membrane protein biomarkers typically fails to meet the clinical requirement for diagnostic accuracy. We investigated the correlation between the membrane protein expression level, the binding kinetics to aptamers and the sizes of EVPs with interferometric plasmonic microscopy (iPM), and demonstrated the implementation of the correlative signature to determine cancer types...
December 2023: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38047476/an-ex-vivo-model-of-interactions-between-extracellular-vesicles-and-peripheral-mononuclear-blood-cells-in-whole-blood
#40
JOURNAL ARTICLE
Blanca V Rodriguez, Yi Wen, Erin N Shirk, Samuel Vazquez, Olesia Gololobova, Amanda Maxwell, Jessica Plunkard, Natalie Castell, Bess Carlson, Suzanne E Queen, Jessica M Izzi, Tom A P Driedonks, Kenneth W Witwer
Extracellular vesicles (EVs) can be loaded with therapeutic cargo and engineered for retention by specific body sites; therefore, they have great potential for targeted delivery of biomolecules to treat diseases. However, the pharmacokinetics and biodistribution of EVs in large animals remain relatively unknown, especially in primates. We recently reported that when cell culture-derived EVs are administered intravenously to Macaca nemestrina (pig-tailed macaques), they differentially associate with specific subsets of peripheral blood mononuclear cells (PBMCs)...
December 2023: Journal of Extracellular Vesicles
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